scholarly journals Prevalence of antibiotic resistant enteric bacteria in the hands of street food vendors in Dhaka city

2018 ◽  
Vol 34 (1) ◽  
pp. 33-38
Author(s):  
Md Mahade Hassan ◽  
Ram Prosad Chakrabarty ◽  
Mohammad Anwar Siddique ◽  
Md Mizanur Rahaman
Keyword(s):  
Public Health ◽  
Foodborne Pathogens ◽  
Food Contamination ◽  
Enteric Bacteria ◽  
Fecal Coliforms ◽  
Personal Hygiene ◽  
Biochemical Tests ◽  
Antibiotic Resistant ◽  
Street Food ◽  
Rdna Sequencing

Foodborne pathogens are affecting public health globally. Street foods being an important part in daily life of urban people are considered as a major source of foodborne pathogens. The major sources of street food contamination are water and the unhygienic condition of the vendors and food preparing places. This study was carried out to assess the load of antibiotic resistant enteric bacteria in the hands of street food vendors. A total of 20 street food vendors were selected from various locations in the Dhaka University campus and their hands were rinsed with 500 mL normal saline. Most of the samples contained high total viable counts, total coliforms count and total fecal coliforms count. A total of 25 selective colonies were identified as enteric bacteria including E. coli, Klebsiella spp. and Acinetobacter spp. by biochemical tests and 16S rDNA sequencing. All these isolates showed high antibacterial resistance against Amoxacilin (64%) and most of them showed sensitivity against Ciprofloxacin (76%) and Azithromycin (72%). This study revealed that the hand hygiene of street vendors is very poor and may have serious implications for public health due to possible contamination of food. This study, therefore, suggests proper training and education of street food vendors in order to improve their knowledge of personal hygiene and sanitation. Bangladesh J Microbiol, Volume 34 Number 1 June 2017, pp 33-38

scholarly journals Microbiological evaluation in street food outside the Universidad del Atlántico North Campus

2019 ◽  
Author(s):  
Nuris Morales Pinto ◽  
Roberto Herrera Acosta ◽  
Diego Llerena Calvo
Keyword(s):  
Food Contamination ◽  
Total Coliform ◽  
Control Measures ◽  
Fecal Coliforms ◽  
Food Handling ◽  
Basic Principles ◽  
Street Food ◽  
Good Manufacturing Practices ◽  
The World ◽  
The University

Background: The street foods are distributed in all the countries of the world, representing a part of the culture and gastronomy of each country, they are ready for consumption and easily acquired. They are exposed to unfavorable conditions that lead to rapid deterioration and increased microbial load. The study consisted of microbiologically evaluating a street food outside the Universidad del Atlántico North Campus.Methods: A survey was applied to know the most consumed food in the university community. In addition, it structured a randomized 3x2 factorial arrangement, where two sampling points will analyze design: Black and Fritos Fritos Juanca, of which 3 samples of the same food were extracted for evaluation of their quality and microbiological safety. Results: Counting microorganisms yielded 16.67% of the samples contaminated by fecal coliforms with 25 CFU / g, total coliform count was present in 50% of the samples with 27.5 and 75.0 results between CFU/ g, whereas 100% of the samples had presence of aerobic mesophiles, and in the total of these there was no presence of sulfite-reducing spores and Staphylococcus aureus.Conclusion: Therefore, all quality control measures must be taken to reduce the growth of microorganisms and prevent food contamination through hygiene techniques, training in health education, basic principles of Good Manufacturing Practices and hygienic practices in food handling.

scholarly journals Detection of enteric bacteria in the popular street food chotpoti in Dhaka, Bangladesh

2017 ◽  
Vol 2 (4) ◽  
pp. 596-602 ◽  
Author(s):  
Md Zakiul Hassan ◽  
Md Saiful Islam ◽  
Md Salauddin ◽  
Abu Hena Abid Zafor ◽  
Meghan L Scott ◽  
...  
Keyword(s):  
Enteric Bacteria ◽  
Test Tube ◽  
Capital City ◽  
Sample Collection ◽  
Biochemical Tests ◽  
Street Food ◽  
Food Borne ◽  
Food Borne Illness ◽  
Colonial Morphology ◽  
Study Sites

Contaminated street food has been linked to food-borne illness and food-borne outbreak. Chotpoti is a popular street food in Bangladesh. This study was conducted to identify enteric bacteria in chotpoti sold in different parts of the capital city Dhaka. From July to October, 2012 we purposively selected 18 sites where chotpoti was sold in Dhaka city. From each site we randomly selected six chotpoti stalls for sample collection. At each stall we aseptically collected approximately 100 gm of a combined sample of chotpoti, chili sauce and water used for cleaning utensils. The combined sample was homogenized and was serially diluted in a sterile test tube with normal saline to obtain a dilution up to 10-5. From each test tube 0.1 ml of dilution was plated on to MacConkey and Blood agar plates under aerobic conditions at 37?C for 24 to 48 hours. Bacterial growth was identified by the colonial morphology, Gram stain, and biochemical tests. Of the 108 samples we analyzed, 84 (78%) were contaminated with bacterial pathogens. Among isolated organisms Acinetobacter (66%) was the most dominant followed by Klebsiella spp. (54%), E. coli (3%) and Proteus spp. (0.9%). Two different organisms were present in 47 (44%) samples. In half of the study sites, every sample was contaminated; in only one site bacterial contamination was absent in all samples. Contaminated street food like chotpoti may cause food borne illness and pose risk of food borne epidemics. Further studies are required for quantitative assessment of pathogens to detect potential sources of contamination and to develop interventions that can effectively reduce street food contamination.Asian J. Med. Biol. Res. December 2016, 2(4): 596-602

scholarly journals Prevalence of Intestinal Parasites and Gastrointestinal Carriage of Pathogenic Gram Negative Enteric Bacteria among Apparently Healthy Food Handlers of Public Hospitals, Addis Ababa, Ethiopia

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Tegegn Belhu ◽  
Kinfe Fissehatsion ◽  
Abraham Tesfaye ◽  
Dr Yohannes Woldekidan ◽  
Kassu Desta
Keyword(s):  
Intestinal Parasites ◽  
Addis Ababa ◽  
Shigella Flexneri ◽  
Enteric Bacteria ◽  
Public Hospitals ◽  
Personal Hygiene ◽  
Diffusion Method ◽  
Food Handlers ◽  
Biochemical Tests ◽  
Disk Diffusion Method

Background. Foodborne diseases are major public health problems in developing countries like Ethiopia. Food handlers with poor personal hygiene working in hospitals could be infected with different intestinal parasites and pathogenic enteric bacteria. Therefore, they could pose a potential risk of foodborne infection to patients and the community. Methods. An institutional based cross-sectional study conducted from March to June 2017. Besides, sociodemographic data were collected using a structured questionnaire, freshly passed stool specimens for direct wet mount smear examination, and formalin ether concentration techniques performed for the detection of parasites. For bacterial identification culture, biochemical tests and antimicrobial sensitivity (Kirby-Baure disk diffusion method) have been performed. Finally, validated data were analyzed using statistical package for social science version 20 (SPSS). Results. From 368 food handlers who participated in the study, 81% were females. 119 (32.34%) were positive for at least one intestinal parasite. The most prevalent parasite was Entamoeba histolytica/dispar 48 (13%), followed by Giardia lamblia 36 (9.78%), Taenia Species 21 (5.7%), Ascaris lumbricoide 8 (2.2%), Trichuris trichiura 5 (1.4%), and Hook worm 1 (0.3%). Regarding the prevalence of enteric bacteria 17(4.6%), food handlers were positive for Salmonella 14 (3.8%) and Shigella flexneri 3 (0.8%). No E. coli O157 : H7 was isolated. All 100% (n = 14) Salmonella isolates were resistant to ampicillin (10 µg) and erythromycin (15 µg). Similarly, 100% (n = 3) of Shigella flexneri isolates were resistant to ampicillin (10 µg) and tetracycline (30 µg). 14.3% (n = 2) Salmonella and 66.7% (n = 2) Shigella flexneri isolates were MDR. Conclusion. The study showed significant carriage of pathogenic microorganisms among food handlers. Therefore, hospital administrators and other stake holders should put measures in place to break chain of transmission routes from silent carrier to other peoples particularly patients at hospital and the community at large.

scholarly journals Hygiene practices among street food vendors in Tamale Metropolis

2016 ◽  
Vol 4 (3) ◽  
pp. 25-30
Author(s):  
F.M. Danikuu ◽  
F.B. Baguo ◽  
O. Azipala
Keyword(s):  
Formal Education ◽  
Food Contamination ◽  
Personal Hygiene ◽  
Food Hygiene ◽  
Biomedical Sciences ◽  
Environmental Sanitation ◽  
Street Food ◽  
Hygiene Practices ◽  
Educational Standard ◽  
Tamale Metropolis

The transmission of food borne infections is on the increase in developing countries and has been linked to the environmental sanitation, personal and food hygiene level and practices in a community. The activities of food vendors can increase the chances of food contamination with pathogenic microorganisms and mycotoxins. The study was conducted to assess the food hygiene practices among street food vendors in the Tamale Metropolis. Structured questionnaires, interviews and field observations were used to assess the age, educational standard, knowledge, food hygiene practices and environmental sanitation of 150 randomly selected street food vendors in Tamale Metropolis from those who agreed to participate. The study noted that street food business in the Tamale Metropolis was women dominated (76%). Majority of vendors (78%) were aged 20-39 years. Public toilets (pit latrines) were accessible to all vending sites. Though high number of street food vendors had some form of formal education (66%) and knowledge of food hygiene (62.7%) certain practices associated with the services, such as not washing hands with soap (12%), serving cooked food with bare hands (38%) and low participation in medical examination (46.7%) are unacceptable. Street food vendors in Tamale Metropolis need regular health education on standard environmental, personal and food hygiene practices and strict enforcement of environmental and food hygiene regulations to keep street food save for consumers.Journal of Medical and Biomedical Sciences (2015) 4(3), 25-30Keywords: Street food, food hygiene practices, personal hygiene, food contamination

scholarly journals Assessment of bacterial contamination of irrigation water and market gardening products at Parakou (A city in northern Benin)

2021 ◽  
Vol 15 (1) ◽  
pp. 241-250
Author(s):  
Eric Bio Nikki Sare ◽  
Armelle Sabine Yélignan Hounkpatin ◽  
Vidédji Naéssé Adjahossou ◽  
Abdel Fawaz Bagoudou ◽  
Anicette Bio Sourou
Keyword(s):  
Escherichia Coli ◽  
Urban Agriculture ◽  
Health Risks ◽  
Irrigation Water ◽  
Bacterial Contamination ◽  
Bacterial Load ◽  
Food Contamination ◽  
Fecal Coliforms ◽  
Biochemical Tests ◽  
Market Gardening

In many districts of Benin, the use of wastewater in urban agriculture is becoming more and more widespread. This activity around wastewater discharges potentially poses health risks to populations. As water is one of the main sources of food contamination in developing countries, the main objective of this study, oriented towards the assessment of the bacterial load, was to search for Salmonella which are pathogenic to humans in irrigation water as well as in some market gardening products consumed in Parakou district. The study was carried out on the market gardening perimeter of the slaughterhouse site located near the international marketArzèkè, where market gardeners exclusively use surface water from the mixture of groundwater and runoff from installed collectors. At the end of this study, the results from the observation of the different colonies, followed by biochemical tests for the detection and differentiation of Salmonella, allowed us to detect the presence of Salmonella in the different samples ranging from 50% to 80%. The presence of Fecal coliforms and Escherichia coli not only in water but also in market garden products was also confirmed. These results might partly explain the frequency of salmonellosis in the study area. Keywords: Salmonella, microbiological tests, biochemical tests.

scholarly journals Bacteriological Status of Street Foods in Different University Premises of Dhaka City, Bangladesh

2019 ◽  
Vol 36 (1) ◽  
pp. 45-47
Author(s):  
Nafisa Tanjia ◽  
Nahida Akhter ◽  
Ajoy Roy ◽  
Mir Shefaly Akhter ◽  
Muniruddin Ahmed ◽  
...  
Keyword(s):  
Food Contamination ◽  
Enteric Bacteria ◽  
Food Samples ◽  
Dhaka City ◽  
Biochemical Tests ◽  
Potential Health ◽  
E Coli ◽  
Food Borne ◽  
The World ◽  
Reasonable Cost

Street food contamination is common and has a potential health hazards throughout the world. These categories of foods are very popular among the students of Academic Institutions (Universities) of Dhaka city, Bangladesh for their appealing look and reasonable cost. This study was conducted to determine the presence of E. coli, Shigella sp. and Vibrio sp. in the street foods. Two hundred and forty-two food samples were collected from 20 University premises of Dhaka city. Biochemical tests were performed on suspected colonies for the identification of the relevant bacteria obtained from the samples. E. coli, Vibrio sp., and Shigella sp. were identified in 18% samples, where E. coli was found in 12%, Vibrio sp. was identified in 5%, and Shigella sp. in <1.0% food sample studied. The samples from which E. coli and food borne pathogens were obtained were considered unsatisfactory for human consumption.Presence of enteric bacteria in street foods indicates that the students of different Universities in Dhaka city might be at high risk of food borne disease Bangladesh J Microbiol, Volume 36 Number 1 June 2019, pp 45-47

scholarly journals Isolation and identification of bacteria in different street vended foods collected from selected areas of Bangladesh

2018 ◽  
Vol 2 (2) ◽  
pp. 65-70
Author(s):  
Torun Kumar Paul ◽  
Snigdha Rani Roy ◽  
Pankaz Roy Sarkar ◽  
Moniruzzaman Tarafder ◽  
Tapu Kumar Saha
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Personal Hygiene ◽  
Public Health Issue ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Street Vendor ◽  
Street Food ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Street food vending has become an important public health issue and a great concern to everybody. This is due to widespread food borne diseases, due to the mushrooming of wayside food vendors who lack an adequate understanding of the basic food safety issues. Major sources contributing to microbial contamination are the place of preparation, utensils for cooking and serving, raw materials, time and temperature abuse of cooked foods and the personal hygiene of vendors. The objective of the study was to explore the microbiological quality of different street food; Chotpoti, Chanachur, Amra (Spondias mombin) and Jolpai (Elaeocarpus serratus (Veralu / Ceylon Olive) sold by various street vendor at Khulna city, Bangladesh. A total of 20 samples were collected randomly from street vendors and tested for the presence of bacteria following standard microbiological method used for isolation, enumaration and identification of bacreria. All the examined samples were contaminated by various types of bacteria. The total viable count (TVC) in different street food samples was ranged from 9.6 x 10 CFU/g to 5.9x10 CFU/g. Among samples Escherichia coli 8(40%), Staphylococcus spp. 5(25%), Klebsiella spp. 4(20%), Salmonella spp. 1(5%) and Shigella spp. 2 (10%) were isolated. Escherichia coli were found highest 3(50%) Jolpai samples, then another organisms Staphylococcus spp. was found highest 2(50%) in Chotpoti and then Klebsiella spp. was found one in each sample. Therefore, application of sound risk analysis policies is being advocated to provide a scientific base to the host of risk management option which Bangladesh may need to explore to ensure public health and safety. Asian Australas. J. Food Saf. Secur. 2018, 2(2), 65-70

scholarly journals Vibrio parahaemolyticus: The protagonist of foodborne diseases

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Vengadesh Letchumanan ◽  
Ke-Yan Loo ◽  
Jodi Woan-Fei Law ◽  
Sunny Hei Wong ◽  
Bey-Hing Goh ◽  
...  
Keyword(s):  
Food Safety ◽  
Foodborne Pathogens ◽  
Vibrio Parahaemolyticus ◽  
Food Contamination ◽  
Control Measures ◽  
Human Consumption ◽  
Antibiotic Resistant ◽  
The People ◽  
Global Health Issue ◽  
Antibiotic Resistant Bacterium

Food contamination is a worrying condition faced by us today. We often discuss on the food safety aspect and how to manage contamination. Food products can be tainted by bacteria at any level of food production to human consumption, subsequently developing gastroenteritis. The people from developed and developing countries are at high risk from harmful effects of unsafe food. Of all the foodborne pathogens, Vibrio parahaemolyticus has been accounted for many outbreaks globally and still at rise even with proper management methods. V. parahaemolyticus infection occurs as a result of improper food handling and preparation, ability of the bacterium to withstand human gut to launch virulence, antibiotic resistant bacterium, and failure of regulatory bodies to safe-guard food quality. This scenario poses a global health issue that warrants rapid control measures to ensure food safety from production to consumption by consumers. For that reason, this review aims to provide an overview of the epidemiology of V. parahaemolyticus as well as discuss the challenges faced to encounter this bacterium.

scholarly journals Molecular Characterization of Enterobacter and Escherichia coli Pathotypes Prevalent in the Popular Street Foods of Dhaka City and their Multidrug Resistance

2019 ◽  
Vol 34 (2) ◽  
pp. 67-72
Author(s):  
Md Belal Hossain ◽  
Nur Dhakirah Binti Mahbub ◽  
Md Miraj Kobad Chowdhury ◽  
Md Mizanur Rahaman
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
16S Rdna ◽  
Restriction Analysis ◽  
Enteric Bacteria ◽  
Global Public Health ◽  
Biochemical Tests ◽  
E Coli ◽  
Food Borne ◽  
Rdna Sequencing

Food borne pathogenic enteric bacteria are of great concern for global public health. Among them, Escherichia coli and Enterobacter spp. are the most prevalent in the street food. In this study, 23 strains of such enteric bacteria were isolated from multiple food samples by conventional cultural technique. Isolated strains were characterized molecularly into different genotypes using RAPD, amplified ribosomal DNA restriction analysis, and partial sequencing of 16S rDNA. RAPD represents 10 different types of strains whereas ARDRA clusters them into two separate groups. 16 out of the 23 isolates were identified as E. coli and the rest were as Enterobacter spp. by biochemical tests and were further confirmed by partial 16S rDNA sequencing. Significant level of virulence traits including stx1, stx2 and escV genes were identified in E. coli strains. Also, most of the isolates were found resistant to azithromycin and amoxicillin. This study revealed the presence of various pathogenic enteric bacteria in various street foods with multidrug resistance. Therefore, this study suggests that people consuming such street foods are at major risk of food borne illness. Bangladesh J Microbiol, Volume 34 Number 2 December 2017, pp 67-72

scholarly journals Is Caretta Caretta a Carrier of Antibiotic Resistance in the Mediterranean Sea?

Antibiotics ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 116 ◽  
Author(s):  
Rosa Alduina ◽  
Delia Gambino ◽  
Alessandro Presentato ◽  
Antonino Gentile ◽  
Arianna Sucato ◽  
...  
Keyword(s):  
Mediterranean Sea ◽  
Sea Turtles ◽  
Caretta Caretta ◽  
Metal Resistance ◽  
Resistant Bacteria ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
Class 1 Integron ◽  
Antibiotic Resistant ◽  
Rdna Sequencing

Sea turtles can be considered a sentinel species for monitoring the health of marine ecosystems, acting, at the same time, as a carrier of microorganisms. Indeed, sea turtles can acquire the microbiota from their reproductive sites and feeding, contributing to the diffusion of antibiotic-resistant strains to uncontaminated environments. This study aims to unveil the presence of antibiotic-resistant bacteria in (i) loggerhead sea turtles stranded along the coast of Sicily (Mediterranean Sea), (ii) unhatched and/or hatched eggs, (iii) sand from the turtles’ nest and (iv) seawater. Forty-four bacterial strains were isolated and identified by conventional biochemical tests and 16S rDNA sequencing. The Gram-negative Aeromonas and Vibrio species were mainly found in sea turtles and seawater samples, respectively. Conversely, the Gram-positive Bacillus, Streptococcus, and Staphylococcus strains were mostly isolated from eggs and sand. The antimicrobial resistance profile of the isolates revealed that these strains were resistant to cefazolin (95.5%), streptomycin (43.2%), colistin and amoxicillin/clavulanic acid (34.1%). Moreover, metagenome analysis unveiled the presence of both antibiotic and heavy metal resistance genes, as well as the mobile element class 1 integron at an alarming percentage rate. Our results suggest that Caretta caretta could be considered a carrier of antibiotic-resistant genes.

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