scholarly journals Effects of alfaprostol and luprostiol on the embryo production within MOET technique in Black Bengal goats

1970 ◽  
Vol 2 (2) ◽  
pp. 147-150 ◽  
Author(s):  
MI Faruk ◽  
BZ Fatema ◽  
FY Bari ◽  
MGS Alam
Keyword(s):  
Treatment Group ◽  
Embryo Transfer ◽  
Treated Group ◽  
Embryo Production ◽  
Recovery Method ◽  
Treatment Groups ◽  
Multiple Ovulation ◽  
Significant Difference ◽  
Surgical Recovery ◽  
The Mean

The effects of Alfaprostol and Luprostiol on embryo production within multiple ovulation and embryo transfer (MOET) technique were studied on 16 Black Bengal goats during the period from January 2002 to June 2003. These 16 goats were randomly divided into two equal groups (A & B), each consisting of 8 goats. Each of the 16 goats was flushed in different times within MOET technique to determine the effects of alfaprostol and luprostiol on embryo production. Each group consisting of 8 donors was synchronized with alfaprostol (Gabbrostim®, VETEM, Italy) or luprostiol (Prosolvin®, Intervet International, Netherlands) @ 2 mg and 7.5 mg, equivalent to 1 ml / donor respectively. The donor goats were hand mated following the onset of oestrus, 1-2 times at 6 h interval depending on the duration of oestrus. The embryos were collected at Day 7 of mating using surgical recovery method. The mean number of ovulation in alfaprostol and luprostiol group was 8.50 ± 0.90 and 8.1 ± 0.76, respectively, where in both cases 900 iu PMSG was used for induction. There was no significant (p > 0.05) difference between this two groups on superovulation rate. The mean numbers of recovered, fertilized and transferable embryos were 5.4 ± 0.80 and 5.1 ± 0.61; 3.9 ± 0.52 and 2.6 ± 0.37 and 3.6 ± 1.6 and 2.4 ± 1.0, respectively, in alfaprostol and luprostiol treatment group. Like superovulation, there was no difference between the two treatment groups on recovered, fertilized and transferable embryos. The percentage of recovered, fertilized and transferable embryos were 63 ± 7.7 and 63 ± 3.74, 72 ± 4.55 and 51 ± 7.0 and 93.6 ± 1.6 and 90.48 ± 1.0 in alfaprostol and luprostiol treated groups, respectively. The significant difference was only existed in the percentages of fertilized embryos between the two treatment groups, where alfaprostol treated group had the significantly (p < 0.01) higher percentage of fertilized embryos.Key words: Alfaprostol; luprostiol; effect; embryo transfer; ovulation; Black Bengal goatsdoi: 10.3329/bjvm.v2i2.2558Bangl. J. Vet. Med. (2004). 2 (2): 147-150

scholarly journals Efficacy of flumethrin pour on against Haematobia exigua infesting cattle under field conditions

2021 ◽  
Author(s):  
Periyasamy Anbarasi ◽  
Gurusamy Ponnudurai ◽  
Kandasamy Senthilvel ◽  
Kuppannan Sukumar ◽  
Palani Sriniva
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Tamil Nadu ◽  
Treatment Intensity ◽  
Treatment Groups ◽  
Significant Difference ◽  
The Mean ◽  
Pre Treatment ◽  
And Control ◽  
Cattle Farms

Abstract The efficacy of flumethrin 1% pour-on (1% w/v, Flumitas) was evaluated against Haematobia exigua on cattle farms in Namakkal district, Tamil Nadu from November 2019 to February 2020. In this study, five farms, which had fly menace, selected randomly were divided as treatment (F1, F2, F3 and F4) and control (Fc) groups. Flies collected from the farms were identified as Haematobia exigua and a mean pre-treatment intensity was 195.56 ± 14.07. In the treatment groups (F1,F2,F3 and F4) flumethrin 1% pour on was applied to the back of cattle at the rate of 1 ml/10 kg body weight and fly intensity was recorded for a period of 2 months. The fly intensity reduced to zero within 30 minutes of application and the same trend continued till 28 DPT in F2 and F3, while it was effective 35 DPT in F1 and F4. A highly significant difference in the mean H. exigua fly counts between control and treatment group (P < 0.0001) was observed.

195 Effect of feeding a licorice extract to Japanese Black cows on embryo production performance after superovulation treatment

2019 ◽  
Vol 31 (1) ◽  
pp. 222
Author(s):  
Y. Aoyagi ◽  
M. Takeuchi ◽  
Y. Oono ◽  
M. Urakawa ◽  
M. Koiwa
Keyword(s):  
Treatment Group ◽  
Glycyrrhizic Acid ◽  
Embryo Quality ◽  
Production Performance ◽  
Control Group ◽  
Embryo Production ◽  
Significant Difference ◽  
Licorice Extract ◽  
The Mean ◽  
Effect Of Feeding

The objective was to determine the effect of feeding a licorice extract that contains glycyrrhizic acid, which is known to have a liver function-enhancing effect, on embryo production performance after superovulation treatment in Japanese Black cows. Japanese Black breed cows (n=136) that had calved 1 to 4 times and had normal uteri as seen by ultrasonography after at least 40 days from the last calving were used as test animals. Animals in the treatment group (n=90) were continuously fed 20g head−1 d−1 of a licorice extract (Kanzou, Fabric Onishi Co. Ltd., Osaka, Japan), with at least 13% glycyrrhizic acid content) once a day, mixed with the formula feed, for 60 to 90 days starting from around the day of calving until ova/embryos were collected. The control group (n=46) received no Kanzou from the day of the last calving until ova/embryo collection. A total of 20 Armour units (AU) of FSH (Antorin R, Kyoritsu Seiyaku Corporation, Kanagawa, Japan) from porcine pituitary was given intramuscularly (IM) twice a day, morning and evening, for 3 consecutive days (decreasing dose schedule: 5 AU×2, 3 AU×2, and 2AU×2) as the superovulation treatment to the cows at 8 to 11 days post-oestrus in both groups. Prostaglandin F2α 25mg and 15mg (IM) were administered in the morning and evening, respectively, on the third day of FSH administration. Artificial insemination was done at 12 and 24h after the start of oestrus, and ova/embryos were collected using a uterine reflux method on Day 7 post-oestrus. The mean numbers of ova/embryos and transferable embryos collected and the mean embryo quality scores (according to the IETS guidelines) in the treatment and control groups were compared by t-test. A chi-squared test was used to compare the proportion of transferable embryos in the 2 groups. There was no significant difference in the mean number of ova/embryos retrieved between the treatment group (21.7±11.8) and the control group (15.8±11.3), although the former tended (P=0.07) to yield more ova/embryos. There was significant difference (P&lt;0.05) in the mean number of transferable embryos collected in the treatment group (13.1±8.3) than in the control group (8.1±5.3). The mean embryo quality score was significantly greater (P&lt;0.01) in the treatment group (1.6±0.4) than in the control group (2.2±0.6). The proportion of transferable embryos in the treatment group (60.3±26.4%) was also significantly higher (P&lt;0.05) than in the control group (51.2±26.0%). In conclusion, feeding a licorice extract to Japanese Black cows for 60 days or longer improves the quality of embryos obtained after superovulation treatment and leads to an increase in the mean number of transferable embryos per cow.

Effect of Furosemide on the Clinical Course of Transient Tachypnea of the Newborn

PEDIATRICS ◽  
1985 ◽  
Vol 75 (5) ◽  
pp. 908-910
Author(s):  
Thomas E. Wiswell ◽  
James S. Rawlings ◽  
Franklin R. Smith ◽  
Elwin D. Goo
Keyword(s):  
Treatment Group ◽  
Prospective Study ◽  
Clinical Course ◽  
Treated Group ◽  
Control Group ◽  
Treatment Groups ◽  
Significant Difference ◽  
Length Of Hospitalization

The effect of furosemide on the course of transient tachypnea of the newborn was evaluated in a controlled, prospective study. Fifty infants with transient tachypnea of the newborn were randomly assigned to control or treatment groups. Those in the treatment group were given furosemide, 2 mg/kg orally, at the time of diagnosis followed by 1 mg/kg 12 hours later if the symptoms persisted. Infants in the control group received a placebo. Compared with infants in the control group, the furosemide-treated group demonstrated no significant difference in the duration of tachypnea nor in the length of hospitalization. It is concluded that oral furosemide, at the doses used in this study, does not significantly affect the clinical course of transient tachypnea of the newborn.

scholarly journals Peningkatan Pengetahuan Ibu-Ibu Rumah Tangga Dalam Perilaku Pengobatan Sendiri Untuk Penatalaksanaan Demam Dengan Metode Cara Belajar Ibu Aktif

2018 ◽  
Vol 1 (1) ◽  
pp. 27-30
Author(s):  
Rezqi Handayani
Keyword(s):  
Treatment Group ◽  
Mean Value ◽  
Pharmacological Therapy ◽  
Control Group ◽  
Test Results ◽  
Self Medication ◽  
Treatment Groups ◽  
Significant Difference ◽  
Level Of Knowledge ◽  
The Mean

Pharmacological therapy in fever patients is to provide Antipyretic drugs, can be done by using a doctor's prescription or by self-treatment. The condition that occurs in the community is self-medication conducted in the category of irrational treatment therapy because it is not in accordance with the condition of the patient. So, it is necessary to do education using Active Mother�s Ways of Learning Method to the housewives about rational fever self-treatment. The sample in this study were 40 housewives in the Flamboyant Bawah, divided into two major groups namely the control and treatment groups. Each group was given a pretest and posttest in a questionnaire of fever management. In the treatment group, fever drug education given before posttest by used CBIA method. The results showed an increase in knowledge of housewives from before education and after education was seen from the acquisition of grades. The mean pretest value in the control group was 74.38 and the treatment group was 69.69. Statistical test results show there was no significant difference in the mean value between the control group and the treatment group. The mean posttest value in the control group was 77.81 and the treatment group was 87.50. There was a significant difference between the mean value of the control group and treatment group. From the results of the statistical analysis showed that the level of knowledge of the treatment group given education by used the CBIA method produced a higher value than the control group that was not given education.

scholarly journals Inhibitory effect of jengkol leaf (Pithecellobium jiringa) extract to inhibit Candida albicans biofilm

2016 ◽  
Vol 49 (3) ◽  
pp. 148
Author(s):  
Muhammad Luthfi ◽  
Ira Arundina ◽  
Nizamiar Hamni
Keyword(s):  
Candida Albicans ◽  
Treatment Group ◽  
Optical Density ◽  
Leaf Extract ◽  
Mean Value ◽  
Optimum Dose ◽  
Control Group ◽  
Treatment Groups ◽  
Significant Difference ◽  
The Mean

Background: Candida albicans (C. albicans) are dimorphic fungi in oral cavity, considered not only as normal flora, but also as pathogens. C. albicans have an ability to grow biofilm, which has a thick layer of outer skin structure, called as extracellular matrix. Jengkol leaves (Pithecellobium jiringa) contain alkaloids, flavonoids, terpenoids, and lectins, which have an ability as antifungal agent Purpose: This study aimed to analyze the optimum dose of jengkol leaf extract as antibiofilm against C. albicans biofilms. Method: C. albicans were cultured on yeast peptone dextrosa (YPD) media in 96-well microtiter plate flat bottom plates. There were one control group (without treatment) and three treatment groups. The first treatment group was given jengkol leaf extract at a dose of 100 mg/ ml. The second treatment group was given jengkol leaf extract at a dose of 200 mg/ ml. And, the third treatment group was given jengkol leaf extract at a dose of 400 mg/ ml. Semi quantitative method was applied to determine C. albicans biofilmsis using Crystal Violet staining technique. The absorbance of the cells then was calculated using a spectrophotometer with a wavelength of 570 nm. Result: The mean value of optical density in the control group was 1.23. The mean value of optical density in the treatment group with a dose of 100 mg/ ml was 0.2. Meanwhile, the mean value of optical density in the treatment group with a dose of 200 mg/ ml was 0.2, and 0.21 in the treatment group with a dose of 400 mg/ ml. The results also showed that there were significant differences between the control group and all of the treatment groups (p<0.05), but there was no significant difference between the treatment groups (p>0.05). Conclusion: The optimum dose of jengkol leaf extract used as antibiofilm against C. albicans biofilms is 100 mg /ml with an inhibitory percentage of 83.7%.

scholarly journals Effects of imidacloprid and thiamethoxam on the development and reproduction of the soybean aphid Aphis glycines

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0250311
Author(s):  
Aonan Zhang ◽  
Lin Zhu ◽  
Zhenghao Shi ◽  
Tianying Liu ◽  
Lanlan Han ◽  
...  
Keyword(s):  
Treatment Group ◽  
Reproductive Rate ◽  
Soybean Aphid ◽  
Aphis Glycines ◽  
Intrinsic Rate Of Increase ◽  
Control Group ◽  
Net Reproductive Rate ◽  
Treatment Groups ◽  
Rate Of Increase ◽  
The Mean

The soybean aphid Aphis glycines Matsumura (Hemiptera: Aphididae) is a primary pest of soybeans and poses a serious threat to soybean production. Our studies were conducted to understand the effects of different concentrations of insecticides (imidacloprid and thiamethoxam) on A. glycines and provided critical information for its effective management. Here, we found that the mean generation time and adult and total pre-nymphiposition periods of the LC50 imidacloprid- and thiamethoxam-treatment groups were significantly longer than those of the control group, although the adult pre-nymphiposition period in LC30 imidacloprid and thiamethoxam treatment groups was significantly shorter than that of the control group. Additionally, the mean fecundity per female adult, net reproductive rate, intrinsic rate of increase, and finite rate of increase of the LC30 imidacloprid-treatment group were significantly lower than those of the control group and higher than those of the LC50 imidacloprid-treatment group (P < 0.05). Moreover, both insecticides exerted stress effects on A. glycines, and specimens treated with the two insecticides at the LC50 showed a significant decrease in their growth rates relative to those treated with the insecticides at LC30. These results provide a reference for exploring the effects of imidacloprid and thiamethoxam on A. glycines population dynamics in the field and offer insight to agricultural producers on the potential of low-lethal concentrations of insecticides to stimulate insect reproduction during insecticide application.

scholarly journals Topiramate Versus Propranolol in the Prophylaxis of Migraine in Bangladeshi Population

2020 ◽  
Vol 18 (2) ◽  
pp. 12-17
Author(s):  
Muhammad Tayeb ◽  
Md Hasanuzzaman ◽  
Abul Mansur Md Rezaul Karim ◽  
Mohammad Sanaullah ◽  
Md Ashraful Islam
Keyword(s):  
Migraine Attack ◽  
Divided Dose ◽  
Low Dose ◽  
Primary Headache ◽  
Headache Disorder ◽  
Treatment Groups ◽  
Primary Headache Disorder ◽  
Significant Difference ◽  
The Mean ◽  
Bangladeshi Population

Background : Migraine is primary headache disorder characterized by recurring attacks of pain and associated symptoms. The management modality is still unsatisfactory due to poor understanding of its cause and pathogenesis. To assess the efficacy and safety of low dose Topiramate vs Propranolol in migraine prophylaxis. Materials and methods : A randomized clinical trial including 130 patients with frequent migraine headache >5 attacks per month was performed in the out patients Department of Medicine and Neurology, CMCH for a period of 12 weeks. The patients were randomly divided into two treatment groups – treated by Topiramate 50mg/day and Propranolol 80mg/day respectively. Topiramate started with 25mg/day for 7 days then increased up to 50mg/day in two divided dose. Propranolol started with 40mg/day for 7 days then increased up to 80mg/day in two divided dose. The patients were assessed at 0, 8 and 12 weeks of the study. Results: The Topiramate group showed a reduction in the mean (±SD) of frequency of migraine attack from 6.95(±2.88) to 1.75(±1.08) episode per month, headache days per month from 7.62(±4.14) to 1.83(±1.10), intensity of headache per attack from 8.98(±1.05) to 6.10(±2.50) based on VAS scale, duration of headache per episode from 11.56(±9.16) to 5.40(±2.97) per hour and MIDAS score from 16.19(±3.91) to 8.14(±3.93). In patient treated with Propranolol, the mean (±SD) of monthly frequency of migraine attack declined from 7.09(±2.87) to 1.92(±0.98) episode per month, headache days per month from 8.17(±4.52) to 1.86(±o.83), intensity of headache per attack from 8.47(±1.10) to 6.03(±2.05) based on VAS scale, duration of headache per episode from 11.16(±8.08) to 5.97(±3.45), MIDAS score from 15.48(±3.55) to7.77(±3.49). Pre- and post-treatment values were significantly different for individual groups but no significant difference observed between groups. Conclusion: This study demonstrated that both low dose Topiramate and propranolol could significantly reduce migraine frequency, intensity and duration. Chatt Maa Shi Hosp Med Coll J; Vol.18 (2); July 2019; Page 12-17

scholarly journals Anti-Inflammatory Effect of Musa acuminata Stem

2020 ◽  
Vol 14 (02) ◽  
pp. 294-298
Author(s):  
Maharani Laillyza Apriasari ◽  
Selviana Rizky Pramitha ◽  
Dewi Puspitasari ◽  
Diah Savitri Ernawati
Keyword(s):  
Treatment Group ◽  
Musa Acuminata ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Tnf Α ◽  
Significant Difference ◽  
Anti Inflammatory ◽  
Mucosal Wound ◽  
Inflammatory Effect

Abstract Objective This study was designed to assess the anti-inflammatory effect of Musa acuminata through the expression of tumor necrosis factor-α (TNF-α) and nuclear factor kappa β (NF-κB) after 3 days of application of Musa acuminata stem extract (MASE) gel on oral mucosal wound. Materials and Methods An experimental study with post-test only control group design was conducted. Twenty male Rattus norvegicus (Wistar) were injured on their left buccal mucosa and treated three times a day with MASE gel of varying concentrations: 0% (as control), MASE 25%, MASE 37.5%, and MASE 50%. On day 3, a biopsy was performed on each mucosal wound for later immunohistochemical analysis for the expressions of TNF-α and NF-κB. Results The highest expression of TNF-α was observed in the control group (13.20 ± 1.79), while the lowest was in the treatment group using 50% MASE (6.40 ± 1.14). Meanwhile the comparison between treatment groups did not highlight any significant difference (p > 0.05). The highest expression of NF-κB was observed in the control group (13.20 ± 1.30), whereas the lowest was in the treatment group using MASE 50% (6.40 ± 1.14). NF-κB was significantly lower in the treatment group using MASE 50% when compared with other treatment groups (p < 0.05). Conclusion Application of MASE on mucosal wound reduces the expression of TNF-α and NF-κB at all concentrations. The anti-inflammatory effect of MASE 50% was the strongest one.

scholarly journals Dentofacial Changes after Orthodontic Intervention with Eruption Guidance Appliance in the Early Mixed Dentition

2008 ◽  
Vol 78 (2) ◽  
pp. 324-331 ◽  
Author(s):  
Katri Keski-Nisula ◽  
Leo Keski-Nisula ◽  
Hannu Salo ◽  
Kati Voipio ◽  
Juha Varrela
Keyword(s):  
Treatment Group ◽  
Mixed Dentition ◽  
Class I ◽  
Control Group ◽  
Significant Shift ◽  
Facial Height ◽  
Significant Difference ◽  
The Mean ◽  
Maxillary Incisors ◽  
Clinically Significant

Abstract Objective: To evaluate skeletal and dentoalveolar changes induced by the eruption guidance appliance in the early mixed dentition. Materials and Methods: Pre- and posttreatment cephalometric radiographs of 115 consecutively treated children, 62 boys and 53 girls, were compared with those obtained from a control group of 104 children, 52 boys and 52 girls. Pretreatment radiographs were taken at the deciduous-mixed dentition interphase (T1) and after full eruption of all permanent incisors and first molars (T2). The mean age of the children in both groups was 5.1 years at T1 and 8.4 years at T2. Results: A significant difference between the groups at T2 was found in the mandibular length, midfacial length, and maxillomandibular differential. The increase in mandibular length was 11.1 mm in the treatment group and 7.2 mm in the control group. No differences were found in measurements of maxillary position or size. There was a significant shift toward a Class I relationship in the treatment group. Labial tipping and linear protrusion of the mandibular incisors was evident in the treatment group at T2. There was no effect on the inclination or position of the maxillary incisors. Conclusions: Occlusal correction was achieved mainly through changes in the dentoalveolar region of the mandible. In addition, the appliance enhanced condylar growth resulting in a clinically significant increase in mandibular length. No effect was observed on maxillary position, maxillary size, inclination or protrusion of the maxillary incisors, or facial height.

314 RELATIONSHIP BETWEEN SERUM ANTI-MULLERIAN HORMONE (AMH), OVARIAN RESERVE, AND EMBRYO PRODUCTION IN SUPEROVULATED HOLSTEIN COWS

2013 ◽  
Vol 25 (1) ◽  
pp. 304 ◽  
Author(s):  
A. Rozner ◽  
J. Verstegen
Keyword(s):  
Embryo Production ◽  
Blood Samples ◽  
Positive Correlation ◽  
Embryo Recovery ◽  
Multiple Ovulation ◽  
Oocyte Collection ◽  
Significant Difference ◽  
Collection Data ◽  
Over Time

The relations between serum anti-Mullerian hormone (AMH), oocyte numbers, and in vivo embryo production in Holstein heifers were evaluated. The AMH levels of 15 unstimulated cows were followed at weekly intervals during their oestrous cycles and monthly for 4 months. Forty-one superovulated heifers were evaluated at ovum pick-up (OPU) performed 20 h after cystorelin administration, and 125 others were evaluated at embryo recovery. Animals were followed over 3 consecutive cycles induced using a modified Ovsynch protocol with 4 days of FSH (Pluset H, Minitube of America, Verona, WI, USA). Blood samples were collected in serum tubes and spun within 2 h. The samples were stored at –20°C until evaluation using the Minitube of America AMH-bovine specific immunoassay (AMH Fertility Assay™). The statistical analyses (ANOVA and data correlation) were performed using Statview 5 with P < 0.05. Serum AMH ranged from 43 to 960 pg mL–1. The average AMH level of all cows was stable during the oestrous cycle and for each of the 4 monthly measurements. There was a high correlation between all values per animal (r2 = 0.9077; P < 0.01), suggesting that AMH levels are consistent during the cycle and for at least 4 consecutive months. Animals that were repeatedly stimulated showed decreasing AMH levels (509 ± 295, 299 ± 210, 211 ± 119) and a decrease in recovered embryos (7.4 ± 4, 5.6 ± 3.8, 4.2 ± 3.2; P = 0.02). The number of oocytes was not altered by multiple stimulations (10.4 ± 9.8, 11.3 ± 6.2, 8.5 ± 7.6; P = 0.75). As AMH and embryo numbers decreased after multiple stimulations, only the first AMH value and results of the first OPU or flush were used to establish following correlation. Serum AMH showed a positive correlation to the number of oocytes (r2 = 0.245) and embryos collected (r2 = 0.27).When separated into AMH categories, low (<100), normal (100–400), and high (>400 pg mL–1), high-AMH OPU animals yielded significantly higher numbers of oocytes than the animals in the normal or low AMH groups (13.8 ± 9.2 v. 9.2 ± 5.2 and 5.6 ± 3.9; P = 0.001). Flushed animals with high AMH levels had significantly higher numbers of embryos than those with low AMH (10.9 ± 7.9 v. 5.7 ± 5; P = 0.002). Comparison of the first AMH value to the average number of oocytes or embryos collected over the course of 3 collections/animal showed a positive correlation to the average number of oocytes/collection from individual OPU donors (r2 = 0.436) and a positive correlation to the average number of embryos/collection from individual donors (r2 = 0.176). When separated into AMH groups, high-AMH flushed animals had significantly higher numbers of embryos than the normal- or low-AMH animals (9.3 ± 3.1 v. 5.7 ± 3.4 and 4.5 ± 2; P = 0.0001). As OPU animals with low AMH were used only once, average oocyte/collection data was not available for this category. A significant difference was observed between the high- and normal-AMH categories (12 ± 3.6 v. 7 ± 2; P = 0.0001). Circulating AMH is stable over time in unstimulated animals but decreases in repetitively stimulated animals. Anti-Mullerian hormone is highly associated with superovulation response and oocyte and embryo production, and its use should improve animal selection to achieve improve efficiency of multiple-ovulation embryo transfer.

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