scholarly journals Bacterial contamination of street-vended spicy puffed-rice sold at Bangladesh Agricultural University campus

2015 ◽  
Vol 31 (1) ◽  
pp. 20-26 ◽  
Author(s):  
A Khandoker ◽  
MA Islam ◽  
MM Rahman ◽  
AA Husna ◽  
S Das ◽  
...  
Keyword(s):  
Bacterial Contamination ◽  
Health Hazard ◽  
Antibiotic Sensitivity ◽  
Botanical Garden ◽  
Total Coliform ◽  
Viable Count ◽  
Street Vendors ◽  
E Coli ◽  
Puffed Rice ◽  
Staphylococcus Spp

This study was undertaken to investigate the bacterial contamination of spicy puffed rice (Jhalmuri) sold by the street vendors at Bangladesh Agricultural University (BAU) campus. Fifteen spicy puffed rice samples were collected from street vendors at the Botanical Garden, Library premises, Riverside, Krishi Bishhobiddaloy (KB) High School and Veterinary Teaching Hospital compound at BAU campus. Microbial quality was assessed by total viable count (TVC), total coliform count (TCC) and total staphylococcal count (TSC). Samples were inoculated into selective media Eosin Methylene Blue (EMB) agar, Salmonella Shigella (SS) agar, Thiosulphate Citrate Bile Salts Sucrose (TCBS) agar and Mannitol Salt (MS) agar. E. coli and Staphylococcus spp. were identified from the samples. The TVC in spicy puffed rice sample ranged from log 4.5 cfu/g to log 5.4 cfu/g, TSC ranged from log 4.4 cfu/g to log 5.2 cfu/g and TCC ranged from log 1.4 cfu/g to log 4.3 cfu/g. Antibiotic sensitivity test showed that the isolates were sensitive to ciprofloxacin and gentamicin. E. coli were resistant to ampicillin, chloramphenicol and cephalexin and Staphylococcus spp. were resistant to ampicillin, cephalexin and vancomycin. Spicy puffed rice sold by the street vendors at BAU campus harboured multidrug resistant food borne bacteria which may cause public health hazard. DOI: http://dx.doi.org/10.3329/bvet.v31i1.22839 Bangl. vet. 2014. Vol. 31, No. 1, 20-26

Food Safety Knowledge of Consumers and the Microbiological and Temperature Status of Their Refrigerators

2005 ◽  
Vol 68 (7) ◽  
pp. 1421-1430 ◽  
Author(s):  
J. KENNEDY ◽  
V. JACKSON ◽  
I. S. BLAIR ◽  
D. A. McDOWELL ◽  
C. COWAN ◽  
...  
Keyword(s):  
Food Safety ◽  
Bacterial Contamination ◽  
Total Coliform ◽  
Safety Education ◽  
Food Safety Education ◽  
E Coli ◽  
Knowledge Questionnaire ◽  
Survey Results ◽  
Food Safety Risk ◽  
Safety Knowledge

The objectives of this study were to examine domestic food safety knowledge levels of consumers, establish the levels and incidence of bacterial contamination and operational temperatures in domestic refrigerators, and identify areas in which consumer food safety education is necessary in Ireland. A food safety knowledge questionnaire applied to a representative sample of households (n = 1,020) throughout the island of Ireland found the gaps in consumer food safety knowledge. Analysis of swab samples (n = 900) recovered from the domestic refrigerators in these households showed average total viable counts of 7.1 log CFU/cm2 and average total coliform counts of 4.0 log CFU/cm2. Analysis of swab samples also detected the incidence of Staphylococcus aureus (41%), Escherichia coli (6%), Salmonella enterica (7%), Listeria monocytogenes (6%), and Yersinia enterocolitica (2%). Campylobacter jejuni and E. coli O157:H7 were not detected in domestic refrigerators. The temperature profiles of a subset of the sampled refrigerators (100) were monitored for 72 h, and 59% were found to operate, on average, at temperatures above the recommended 5°C. Knowledge and temperature survey results varied considerably, but consumers who scored better in terms of basic food safety knowledge had reduced levels of bacterial contamination in their refrigerators and reported a reduced incidence of food-associated illnesses. This study confirms the effect of basic food hygiene knowledge on hygienic practice and identifies specific areas for emphasis in the development and delivery of effective food safety risk communication messages to consumers.

scholarly journals Bacterial Analysis and Survey of the Street Food of Kathmandu in Relation to Child Health

2015 ◽  
Vol 26 ◽  
pp. 1-9
Author(s):  
R Tuladhar ◽  
Anjana Singh
Keyword(s):  
Bacterial Contamination ◽  
Enterobacter Aerogenes ◽  
Food Samples ◽  
Hygiene Practice ◽  
Street Vendors ◽  
Street Food ◽  
E Coli ◽  
Food Borne ◽  
Food Borne Illness ◽  
Dominant Bacteria

Analysis of street foods of Kathmandu for bacterial contamination was performed in 12 different street foods. The surveillance study was carried in 200 children of primary grade from public school and 12 street vendors for the health hygiene and hazards associated with street food. Poor hygiene practice in preparation and handling of street food has been observed in the vendors. The lack of the knowledge in vendors about the source of bacterial contamination and absence of surveillance on street food has subjected street food to the high potential for food borne illness. The inadequate safety measure adopted by the targeted consumers of street food, the children, has augmented the risk associated with street food. All the food samples analyzed were contaminated with bacteria. The mesophilic count was recorded highest in Panipuri while as coliform count was highest in Chana tarkari. The least count of both was observed in Aaloo chop . Highest number of Staphylococccus aureus was found in Kerau (1.5X103cfu/g) and lowest in Momo (8.3 cfu/g). The dominant bacteria contaminating the food was S. aureus followed by Bacillus alvei, Escherichia coli, Enterobacter aerogenes, Bacillus subtilis, Serratia sp., S. saprophyticus. The contaminated hand and clothing of the person who prepare food are the major source of S. aureus. Highest percentage of E. coli found in Panipuri must be due to the use of contaminated water. Chana chatpate and Chana tarkari were the foods found to be contaminated with Salmonella sp. The type of food and the degree of hygiene practice adopted by vendor refl ects the type and magnitude of bacterial contamination. Implementation of hygienic practices in vendors may reduce the contamination of street food and health education of the school children will curtail the incidences of food borne illness. Periodical monitoring of quality of street food will avoid any future outbreaks of bacterial pathogen.J. Nat. Hist. Mus. Vol. 26, 2012: 1-9

scholarly journals Is it Possible to Sanitize Athletes' Shoes?

2015 ◽  
Vol 50 (2) ◽  
pp. 126-132 ◽  
Author(s):  
Gabriele Messina ◽  
Sandra Burgassi ◽  
Carmela Russo ◽  
Emma Ceriale ◽  
Cecilia Quercioli ◽  
...  
Keyword(s):  
Fungal Infections ◽  
Bacterial Count ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Total Bacterial Count ◽  
E Coli ◽  
Enterococcus Spp ◽  
Before And After ◽  
Unpleasant Odor ◽  
Staphylococcus Spp

Context: Footwear should be designed to avoid trauma and injury to the skin of the feet that can favor bacterial and fungal infections. Procedures and substances for sanitizing the interior of shoes are uncommon but are important aspects of primary prevention against foot infections and unpleasant odor. Objective: To evaluate the efficacy of a sanitizing technique for reducing bacterial and fungal contamination of footwear. Design: Crossover study. Setting: Mens Sana basketball team. Patients or Other Participants: Twenty-seven male athletes and 4 coaches (62 shoes). Intervention(s): The experimental protocol required a first sample (swab), 1/shoe, at time 0 from inside the shoes of all athletes before the sanitizing technique began and a second sample at time 1, after about 4 weeks, April 2012 to May 2012, of daily use of the sanitizing technique. Main Outcome Measure(s): The differences before and after use of the sanitizing technique for total bacterial count at 36°C and 22°C for Staphylococcus spp, yeasts, molds, Enterococcus spp, Pseudomonas spp, Escherichia coli, and total coliform bacteria were evaluated. Results: Before use of the sanitizing technique, the total bacterial counts at 36°C and 22°C and for Staphylococcus spp were greater by a factor of 5.8 (95% confidence interval [CI] = 3.42, 9.84), 5.84 (95% CI = 3.45, 9.78), and 4.78 (95% CI = 2.84, 8.03), respectively. All the other comparisons showed a reduction in microbial loads, whereas E coli and coliforms were no longer detected. No statistically significant decrease in yeasts (P = .0841) or molds (P = .6913) was recorded probably because of low contamination. Conclusions: The sanitizing technique significantly reduced the bacterial presence in athletes' shoes.

scholarly journals Quality Analysis of Dhaka WASA Drinking Water: Detection and

2012 ◽  
Vol 4 (2) ◽  
pp. 41-49 ◽  
Author(s):  
KR Mahbub ◽  
A Nahar ◽  
MM Ahmed ◽  
A Chakraborty
Keyword(s):  
Drinking Water ◽  
Supply Chain ◽  
Tap Water ◽  
Microbiological Quality ◽  
Antibiotic Sensitivity ◽  
Viable Count ◽  
Quality Analysis ◽  
E Coli ◽  
Water Detection ◽  
Sensitivity Pattern

KR Mahbub added as the First author on 08/08/2012 and corrected PDF loaded on 08/08/2012.The aim of the study was to assess the microbiological quality of Dhaka WASA drinking water. A total of 45 samples were collected from different outlets of WASA water supply chain. Among the these samples 29 samples were collected from house tap, 5 samples from street pipe line tap and 11 samples from WASA source pump. The results of the Total Viable Count (TVC) showed that 62 % samples of house tap water, 60 % pipeline water and 45.45 % WASA pump water were exceeded the BDS standard (1240:2001) and WHO Guideline for drinking. The highest count was 2 × 106 cfu/ml in the house tap water of Gandaria. Total coliform and E. coli count ranged from <1.8 (MPN) /100 ml to >1600 (MPN)/100 ml. Among all the tested samples, 57.78 % water samples were positive for coliform and 51.11 % samples were positive for E. coli bacteria. Out of twenty three E. coli isolates, 8 isolates were subjected to biochemical and microscopic examination for confirmation. All 8 isolates were detected as E. coli based on biochemical parameters. The antibiotic sensitivity pattern of those isolates was determined. Most of them were found resistant to Ampicillin, Amoxicillin, kanamycin, Penicillin, Sulphomethoxazole antibiotics. Nearly all of them were found sensitive to Gentamycin and Nalidixic acid. The samples collected from different house tap water and road side tap water were more contaminated than WASA source pump water. It may therefore be concluded that distribution lines of Dhaka WASA supply chain might be the main source of microbiological contamination of drinking water. In this regard further investigations with more representatively drawn samples are required.DOI: http://dx.doi.org/10.3329/jesnr.v4i2.10133J. Environ. Sci. & Natural Resources, 4(2): 41-49, 2011

scholarly journals Microbiological analysis of pasteurized and fresh fruit juice sold in Rawalpindi of Pakistan

2013 ◽  
Vol 48 (3) ◽  
pp. 185-192 ◽  
Author(s):  
SA Batool ◽  
SS Tahir ◽  
N Rauf ◽  
R Kalsoom
Keyword(s):  
Fruit Juice ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Viable Count ◽  
Fruit Juices ◽  
Local Market ◽  
Microbiological Analysis ◽  
E Coli ◽  
Bacteria And Fungi

Freshly prepared and pasteurized fruit juices sold by vendors in local market of Rawalpindi city from 10 locations were analyzed for the microbiological quality. Total viable count (TVC), total coliform, faecal coliform, molds and the presence of pathogenic microorganisms such as E. coli, Pseudomonas, Staphylococcus aureus, Salmonella, and fungi like Aspergillus, Pencillum, Rhizopus were determined .In open fruit juices available in city were highly contaminated with bacteria and fungi. E. coli, Salmonella, Staphylococcus, and Pseudomonas were isolated with different frequency. Aspergillus, Pencillum and Rhizopus were also found in juices especially Aspergillus was with high percentages. The pasteurized juices have less contamination as compared to the fresh juices samples. The number and type of microorganisms recovered from the freshly squeezed fruit juices made them unsafe for drinking. The results of this study demonstrate the unhygienic quality of popular types of market vended freshly squeezed fruit juices and their risk to the consumers. DOI: http://dx.doi.org/10.3329/bjsir.v48i3.17329 Bangladesh J. Sci. Ind. Res. 48(3), 185-192, 2013

scholarly journals ANALISA ORGANOLEPTIK DAN MIKROBIOLOGI AMDK DAN AMIU YANG DIJUAL SEKITAR KAMPUS UMKU

2021 ◽  
Vol 2 (1) ◽  
pp. 7
Author(s):  
Yunita Rusidah ◽  
Lailatul Farikhah
Keyword(s):  
Drinking Water ◽  
Normal Condition ◽  
Bacterial Contamination ◽  
Total Coliform ◽  
Water Requirements ◽  
E Coli ◽  
Bottled Drinking Water ◽  
Microbiological Parameters ◽  
The Difference

This study is intended to find the quality of bottled drinking water (AMDK) and drinking water (AMIU) sold around University of Muhammadiyah Kudus, in terms of organoleptic and microbiological parameters in drinking water according to SNI 01-3554-2006 and PMK No. 492 of  2010. The test is carried out using 10 sample of drinking water. They are 7 sample of AMDK (Ades, Airmu, Aqua, Cleo, Crystalin, Le mineral and Vit) and 3 sample of AMIU (Pasuruhan, Prambatan and Purwosari depots). The research consists of organoleptic and  mikrobiologi (TPC, presence of E. coli and total coliform). The results of research shows that the organoleptik of all samples is in normal condition. Whereas, microbiologically, the TPC results before dilution were only Cleo and Ades which were suitable for consumption because they were absent from bacterial contamination. While the TPC results after dilution, testing the difference between E. Coli and Total coliform resulted in 7 samples of bottled drinking water which were safe and feasible as drinking water according to SNI 01-3553-2006 and 3 samples AMIU does not fulfil PMK No. 492/2010, as drinking water requirements.

scholarly journals Isolation and Characterization of Microflora from Human Urine

2014 ◽  
Vol 2 (1) ◽  
pp. 15-18
Author(s):  
Kohinoor Parvin ◽  
Nahid Rahman ◽  
M Mostafizur Rahman ◽  
M Alimul Islam
Keyword(s):  
Escherichia Coli ◽  
Human Urine ◽  
Bacterial Flora ◽  
Research Work ◽  
Antibiotic Sensitivity ◽  
Diabetic Patients ◽  
E Coli ◽  
Isolation And Characterization ◽  
Bacillus Spp ◽  
Staphylococcus Spp

The research work was conducted to isolate & characterized the bacteria isolated from mid-stream urine of human. A total of 30 samples were collected from patients with urinary tract infection (n=10samples); diabetic patients (n=10samples) & apparently healthy individuals (n=10samples). Escherichia coli, Staphylococcus spp. and Bacillus spp. was the predominant bacterial flora of human urine. Among the isolates, the prevalence of Escherichia coli was highest (80%) compared to Staphylococcus spp.(14%) and Bacillus spp.(6%). E. coli isolated from 6 different sources were found to be highly virulent, moderately virulent, less virulent and avirulent categories as observed in day-old suckling mice. Antibiotic sensitivity profiles suggest that nalidixic acid will be the first drugs of choice to treat the UTI caused by E. coli and arythromycin, ampicillin and azithromycin will be the second drugs of choice to treat the UTI caused by Staphylococcus spp and Bacillus spp. respectively. Microbes and Health, June 2013, 2(1): 15-18DOI: http://dx.doi.org/10.3329/mh.v2i1.17257

scholarly journals Seasonal variation of bacterial load in river and lake waters of Dhaka city, Bangladesh

2019 ◽  
Vol 35 (2) ◽  
pp. 116-121
Author(s):  
Md Suprio Sadat Sikdar ◽  
Maruf Abony ◽  
Tamanna Zerin ◽  
Avijit Banik ◽  
Suvamoy Datta
Keyword(s):  
Bacterial Load ◽  
Alcaligenes Faecalis ◽  
Total Coliform ◽  
Viable Count ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
E Coli ◽  
Coliform Count ◽  
Lake Waters ◽  
Analysis Of Waters

Surface water is often contaminated due to human excreta and urban and industrial pollution. The increased population growth and industrialization are likely to exacerbate the situation. Microbiological analysis of waters from rivers (Turag and Buriganga) and lakes (Banani and Dhanmondi) around Dhaka city were conducted for Spring, Monsoon and Fall seasons of the year, 2016 and 2017. Total Viable Count (TVC), Total Coliform Count (TCC), Total Fecal Coliform Count (TFCC) and Total Salmonella Shigella Count (TSSC) were conducted to obtain bacterial load in both enriched and without enrichment water samples. All the Isolates were identified by microscopy, cultural characteristics and biochemical methods. Most of the water reservoirs have a decline in the bacterial load in monsoon where the microbial load of pathogens is highest during spring. Without enrichment, E. coli, Enterobacter sp., Klebsiella sp. and S. aureus were abundant, where others like Shigella sp., Proteus sp., Serratia sp., Pseudomonas sp., Acinetobacter sp., and Alcaligenes faecalis mostly exhibited growth following enrichment. Results indicate that pathogenic environmental isolates can cause serious health issue if water is left untreated or poorly treated from reservoirs within and around Dhaka city. Bangladesh J Microbiol, Volume 35 Number 2 December 2018, pp 116-121

Cytological and Microbiological Analysis of Canine Otitis Externa

2020 ◽  
Author(s):  
V V Karnad ◽  
K Jeyaraja ◽  
K Vijayarani ◽  
S Vairamuthu ◽  
S Subapriya ◽  
...  
Keyword(s):  
Antibiotic Sensitivity ◽  
Inflammatory Cells ◽  
Microbiological Analysis ◽  
Sensitivity Testing ◽  
Universal Primer ◽  
Malassezia Pachydermatis ◽  
Effective Response ◽  
E Coli ◽  
Bacillus Spp ◽  
Staphylococcus Spp

The ear discharge of fifteen dogs was examined for cytological and microbiological evaluation along with antibiotic sensitivity testing. The ear discharge of ten healthy dogs was also analyzed for comparative data. Cytological analysis showed that inflammatory cells and rod shaped organisms in highly infected cases. Otic microbiota in healthy cases revealed Bacillus spp. and Staphylococcus spp., while in clinical cases Pseudomonas spp., E coli, Klebsiella spp. and Alcaligenes spp., were also identified by using universal primer 16S rRNA PCR. The yeast Malassezia pachydermatis was isolated from ear discharge of all dogs. Among the anti – microbial drugs tested, Gentamicin had the most effective response against the otic microbes, followed by ciprofloxacin while neomycin had the least

scholarly journals Hygiene management in newborn individually housed dairy calves focusing on housing and feeding practices

2020 ◽  
Author(s):  
Céline Heinemann ◽  
Caroline D Leubner ◽  
Jason J Hayer ◽  
Julia Steinhoff-Wagner
Keyword(s):  
Pathogenic Bacteria ◽  
Total Coliform ◽  
Viable Count ◽  
Dairy Calves ◽  
Salmonella Spp ◽  
Protein Residues ◽  
E Coli ◽  
Hygiene Management ◽  
Hygiene Measures ◽  
Calf Rearing

Abstract In calf rearing, the first weeks of life are critical and associated with the highest mortality due to enteric and respiratory diseases. A well-implemented hygiene management can help to protect calves´ health preventively by reducing the load of pathogenic bacteria and interrupting infection chains. The aim of this study was to identify deficiencies in hygiene management of individually housed dairy calves by surveying current practice and examining feeding and housing equipment with different hygiene indicators. On 11 farms, different locations in 2 pens or hutches for individual calf rearing prepared for restocking and 2 feeding buckets per farm, including the inner and outer surfaces of artificial teats, were visually scored for cleanliness and sampled with swabs (housing equipment: n = 167; feeding equipment: n = 120). The sanitation of floors was tested with sock samples (n = 41). A total of 328 samples were analyzed for adenosine triphosphate (ATP) and protein residues, aerobic total viable count (TVC), total coliform count (TCC), Escherichia coli, methicillin-resistant Staphylococcus aureus (MRSA), extended spectrum β-lactamase-producing bacteria (ESBL) and Salmonella spp. After evaluation of these results, the farmers were informed about the findings and trained on improvement in hygiene management personally. The sampling was repeated after one year to detect possible changes in hygiene management. The highest bacterial loads (TVC, TCC and E. coli) were observed in feeding equipment, especially the inner teat of milk feeding buckets. Environmental samples, primarily the sidewalls and back walls of tested pens and hutches, exhibited the lowest bacterial counts and ATP and protein residues. All samples were negative for MRSA and Salmonella spp. In 10.5% of all samples, ESBL was detected, and in 6.8%, ESBL E. coli was detected, predominately in sock samples, followed by feeding equipment samples. Training in hygiene management showed only limited effects. In conclusion, there is still great potential to improve the implementation of hygiene measures in individual calf housing. In particular, more attention should be paid to the cleaning of feeding buckets and artificial teats, as this is a simple means of interrupting the possible spread of pathogens among calves.

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