scholarly journals Comparative Nephroprotective Effects of Extracts of Buchholzia coriacea on some Biochemical Parameters in Carbon Tetrachloride-induced Toxicity in Rattus novergicus

2018 ◽  
Vol 17 (2) ◽  
pp. 227-235
Author(s):  
Godwin C. Ugwu ◽  
Chinagorom L. Okanya ◽  
Jude V. Egbuji ◽  
Jude I. Okwo ◽  
Emmanuel I. Nnamonu ◽  
...  
Keyword(s):  
Adverse Effect ◽  
Uric Acid ◽  
Carbon Tetrachloride ◽  
Medicinal Plant ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Methanolic Extract ◽  
Rat Kidney ◽  
Aqueous Extracts ◽  
Methanolic Extracts

Buchholzia coriacea (wonderful kola) is a medicinal plant that has been used worldwide as an alternative medication to promote human health. Comparative nephroprotective effects of crude seed powder, aqueous and methanolic extracts of B. coriacea in hepatotoxic rats was investigated for 56 days. The crude powder (BCCP), aqueous extract (BCAE) and methanolic extract (BCME) significantly reduced (p<0.05) the levels of creatinine and uric acid and decreased minimally throughout the treatment periods. Total protein increased significantly (p<0.05). Significant decrease (p<0.05) were observed with 200 mg/kg BCAE and BCCP respectively in urea and BUN levels. Noticeable nephroprotective effects may be attributed to the presence of phytochemicals like flavonoids and tannins which act as antioxidants. This study has demonstrated that B. coriacea crude seed powder, methanolic and aqueous extracts caused no adverse effect on the rat kidney and may be recommended for the management of nephrotoxicity Dhaka Univ. J. Pharm. Sci. 17(2): 227-235, 2018 (December)

Hepatoprotective effect of methanolic extracts of Phyllanthus virgatus against carbon tetrachloride-induced liver injury in rats

2016 ◽  
Vol 2 (1) ◽  
pp. 1-7
Author(s):  
Chattu Maheswara Rao
Keyword(s):  
Carbon Tetrachloride ◽  
Serum Level ◽  
Total Protein ◽  
Liver Enzymes ◽  
Methanolic Extract ◽  
Hepatocellular Injury ◽  
Significant Extent ◽  
Methanolic Extracts ◽  
Hepatic Cells ◽  
Glutamate Pyruvate

In this study, the methanolic extract of Phyllanthus virgatus was evaluated against carbon tetrachloride (CCl4) induced hepatotoxicity in rats. The results of this study indicated that Phyllanthus virgatus exhibited moderate protective effect at a dose of 100-200 mg/kg by lowering serum level of liver enzymes such as alanine amino transferees (ALT), glutamate pyruvate transaminases (SGPT), aspartate aminotransferase (AST), glutamate oxaloacetate transaminases (SGOT), and total protein to a significant extent. Further, no significant effects were seen on blood serum level at a dose of 100-200 mg/kg body weight. The highest activity was observed at a dose of 200 mg/kg with a reduction of serum concentration of ALT, AST, total bilirubin and total protein. The methanolic extract of P. virgatus showed significant decrease in the levels of liver enzymes, indicating the protection of hepatic cells thereby protecting against CCl4 induced hepatocellular injury.

PRELIMINARY PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF UDHARA VAYUHARA CHURNA

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (06) ◽  
pp. 64-67
Author(s):  
Maruthi T. Ekbote ◽  
◽  
Rajashekar K. V ◽  
Shankarappa L ◽  
Bharathi D. R. ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Acorus Calamus ◽  
Aqueous Extracts ◽  
Piper Longum ◽  
Polyherbal Formulation ◽  
Methanolic Extracts ◽  
Phytochemical Investigation

Ayurvedic medicines are being used increasingly to fight or prevent common diseases. Udhara vayuhara churna is a polyherbal formulation containing Piper longum (Piperceae) Zingiber officinalis (Zingiberaceae), Elettaria cardamom (Zingiberaceae), Plumbago zeylanica (Plumbaginaceae), Carum carvi (Umbelliferae), Acorus calamus (Araceae) and Embilica officinalis (Euphorbiaceae). Methanolic and aqueous extracts of Udhara vayuhara churna were screened for their antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Aspergillus niger and Candida albicans. The results of antimicrobial activity of aqueous and methanolic extracts of the Udhara vayuhara churna indicated that methanolic extract inhibited the growth of one or more test pathogens than aqueous extract. Udhara vayuhara churna formulation extracts showed a broad spectrum of antimicrobial activity. Phytochemical investigation revealed the presence of tannins, saponins, alkaloids, glycosides, flavonoids and essential oils. This may be due to the multifunctional effect of all the seven plant ingredients of Udhara Vayuhara churn.

Preliminary Phytochemical screening of Persicaria sagittata: Future prospective for kidney stone

2021 ◽  
pp. 4367-4370
Author(s):  
Ankita Sharma ◽  
Ishita Ishita ◽  
Inder Kumar ◽  
Kritika Verma ◽  
Bhumika Thakur ◽  
...  
Keyword(s):  
Uric Acid ◽  
Medicinal Plant ◽  
Kidney Stone ◽  
Methanolic Extract ◽  
Biologically Active ◽  
Phytochemical Screening ◽  
Secondary Product ◽  
Standard Methods ◽  
Active Constituents ◽  
Uric Acid Stones

Medicinal plants have bioactive compounds, which are used to treatments of various diseases. In the present study, Persicaria sagittata medicinal plant was used for the preliminary phytochemical screening. Methanol and petroleum ether were used as a solvent for obtaining the extraction of the plant. Both the extracts were further study for qualitative phytochemical screening using standard methods. Phytochemical screening shows that methanolic extract possesses the presence of tannins, flavonoids, and vitamin A as tannins and flavonoids are helpful for the uric acid stones. The study reveals that medicinal plant provides a basis of its use in medicine, improve to further drugs in the pharmaceutical area, and contains different biologically active constituents, and the secondary product is valuable of further analysis.

scholarly journals Study of some biochemical parameters of vitiligo patients in Bangladesh

2012 ◽  
Vol 47 (2) ◽  
pp. 173-186
Author(s):  
Md Ziaul Amin ◽  
Mohd Hafizur Rahman ◽  
Mohammed A Satter
Keyword(s):  
Uric Acid ◽  
Blood Sugar ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Age Groups ◽  
Age Group ◽  
Random Blood Sugar ◽  
Biochemical Studies ◽  
Average Serum

This study is focus on the biochemical analysis to investigate the condition of different biochemical parameters for the Vitiligo patients. This biochemical analysis deals with the blood serum level of random blood sugar, bilirubin, urea, total protein, SGOT, SGPT, alkaline phosphatase, total cholesterol and uric acids. The increasing some of the biochemical parameters were statistically significant with the age groups. Random blood sugar analysis of the 125 patient's age group 31- 40, 41- 50 and above 50 significantly higher than the control group. No significant changes were observed in serum bilirubin, serum urea, serum creatinine, serum alkaline phosphatase (ALP) and serum asparate amino transferase (AST) level in different age group of Vitiligo patients. In this study, the serum total proteins were slightly decreased in the age group patients below 10 and 11-20 as compared to healthy controls. The average serum total protein level of the below 10 and 11-20 age group patients were 5.31 g/dl and 6.50 g/dl respectively, whereas the controls were 5.95 g/dl and 6.92 g/dl respectively. Serum cholesterol, serum triglycerides and serum uric acid levels were significantly changed in the age group 11-20 and 31-40 compared with the healthy control. The average serum cholesterol level, triglyceride (TG) level and uric acid level were 152.17 mg/dl, 90.27 mg/dl, 4.75 mg/dl and 173.40 mg/dl, 149.95 mg/dl, 5.54 mg/dl respectively for the patients of 11-20 and 31-40 years age group. The controls of these parameters for the 11-20 years and 31-40 years age groups were 150.57 mg/dl, 94.41 mg/dl, 4.28 mg/dl and 156.75 mg/dl, 101mg/dl, 4.77mg/dl respectively. These biochemical studies on Vitiligo indicated that there was no significant changed observed in some biochemical parameters in the different age group of Vitiligo patients but some biochemical parameters like total protein, total cholesterol, triglycerides (TG), uric acid and random blood sugar level were significantly changed in different age group of Vitiligo patients specially among the 11-20 and 31-40 years age group. This biochemical studies would be helpful to know details about the pathological condition of the Vitiligo patients associated with other disease. DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11449 Bangladesh J. Sci. Ind. Res. 47(2), 173-186, 2012  

scholarly journals Effect of Methanolic Extract of Caryota no Seed on Geotactic Behaviour and Fecundity in Drosophila melanogaster

2020 ◽  
pp. 38-48
Author(s):  
Chinonye A. Maduagwuna ◽  
Simeon Omale ◽  
Monday A. Etuh ◽  
Steven S. Gyang
Keyword(s):  
Drosophila Melanogaster ◽  
Total Protein ◽  
Methanolic Extract ◽  
Negative Geotaxis ◽  
Protein Levels ◽  
Methanolic Extracts ◽  
Plateau State ◽  
Minute Duration ◽  
Rate Of Emergence

Aims: To investigate the effect of the methanolic extracts of Caryota no (CN) seeds negative geotaxis, fecundity and acetylcholinesterase (AChE) using Drosophila melanogaster (DM).                                                                                                      Study Design: Experimental design. Place and Duration: Sample: African Centre of Excellence for Phytomedicine Research and Development, University of Jos, Jos Plateau State Nigeria between June 2018 and February 2019                                                                                                  Methodology: 50 flies were exposed in each vial to the following concentrations: 300 mg, 350 mg, 400 mg, 500 mg and 600 mg of methanolic extracts in 5 replicates for 7 days with daily recording of mortality. Total protein assays were carried out by Randox method from the supernatant from homogenized whole flies. In vivo antioxidant activity study was conducted by measuring level of acetylcholinesterase (AChE) enzyme activity from supernatants of whole fly homogenates using a spectrophotometer at specific wavelengths over a 2 minute duration. The values were derived as part of the total protein value. Negative geotaxis was done by the climbing assay and fecundity was examined by rate of emergence of larva after exposure of the flies to treatment. The statistical difference among test groups was presumed at P < .05. Results: The methanolic extract of CN caused nonsignificant (P = .33) decrease in total protein levels compared to the control. There were also nonsignificant decreases in AChE (P = .30) activity, negative geotactic (P = .80) behaviour and nonsignificant increase in fecundity (P = .17) in the methanolic extract-treated flies compared to the controls. Conclusion: It can therefore be concluded that the methanolic extract of Caryota no nonsignificantly improved fertitity and reduced negative geotaxis and AChE activity in DM.

Reversible Nematostatic Effect of Peganum harmala L (Nitrariaceae) on Meloidogyne javanica

2019 ◽  
Vol 6 (1) ◽  
Author(s):  
EL HASSAN MAYAD ◽  
KHADIJA BASAID ◽  
JAMES NICHOLAS FURZE ◽  
NIAMA HEIMEUR ◽  
BTISSAM SENHAJI ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Total Content ◽  
Meloidogyne Javanica ◽  
Aqueous Extracts ◽  
In Vitro Experiments ◽  
Peganum Harmala ◽  
Root Knot Nematode ◽  
Second Stage ◽  
Methanolic Extracts

Meloidogyne javanica is considered as the most damaging nematode of vegetables in Morocco. Eco-friendly bionematicides are urgently required for its control. In vitro experiments were carried out to assess the direct effect of bioproducts of Peganum harmala against M. javanica. The bioassay showed extracts to be nematotoxic. Aqueous extracts of P. harmala exhibited reversible nematostatic activity. The estimated ID50 of the most active product in methanolic extracts was 368ppm. HPLC-MS of the methanolic extract revealed that total content of major alkaloids of P. harmala was approximately 12.162±0.637mg/g. Harmine (8.514±0.521mg/g) is the dominant alkaloid. In conclusion, Peganum harmala has a reversible nematostatic activity on second stage juveniles of M. javanica. The effect of P. harmala is due to its possession of a high content of β-carboline alkaloids, which warrant further experimentation. Bioproducts from P. harmala should be exploited through formulations for management of the root knot nematode.

scholarly journals Hepatoprotective Effect of Corrigiola telephiifolia Pourr. Root Methanolic Extracts against CCl4-Induced Hepatic Damage in Mice

2021 ◽  
Vol 12 (2) ◽  
pp. 2489-2502
Keyword(s):  
Biochemical Parameters ◽  
Methanolic Extract ◽  
Hepatoprotective Activity ◽  
Hepatic Damage ◽  
Hepatoprotective Effect ◽  
Histopathological Analysis ◽  
Medicinal And Aromatic Plants ◽  
Bioactive Substances ◽  
Methanolic Extracts ◽  
Hepatic Cells

Liver disease is a dysfunction that affects all or part of the liver and can lead to death. While the use of medicinal and aromatic plants has been a source of bioactive substances with hepatoprotective properties. This study aims to evaluate the hepatoprotective effect of the methanolic extract of Corrigiola telephiifolia roots on hepatic damage induced by carbon tetrachloride (CCl4) in mice. Animals were treated daily with C. telephiifolia methanolic extract (CTME) at doses of 5 and 10 mg/kg b.w for 60 days. In addition, CCl4 was injected (1 mL/kg, i.p.) for its hepatotoxic effects. At the end of the experiment, the blood of all animals was collected to evaluate biochemical parameters and the liver for histopathological analysis. The administration of CTME showed significant hepatoprotective activity by improving the biochemical parameters and the histological appearance of hepatic cells induced by CCl4. Consequently, C. telephiifolia could be used in the prevention and/or treatment of liver intoxication.

scholarly journals EFFECT OF DIETARY ASPIRIN ON SOME BLOOD BIOCHEMICAL PARAMETERS OF BROILERS REARED IN HIGH DENSITY

2009 ◽  
Vol 33 (1) ◽  
pp. 38-43
Author(s):  
Motthena Mahmoud Shaker
Keyword(s):  
Uric Acid ◽  
Blood Serum ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Serum Glucose ◽  
High Density ◽  
Cholesterol Concentration ◽  
Uric Acid Concentration ◽  
Blood Biochemical Parameters ◽  
Blood Biochemical

The objective of this study was to determine the effect of adding aspirin to the diet on somebiochemical parameters such as blood serum glucose , cholesterol , uric acid , total protein ,phosphorus and calcium concentrations of broilers reared in high density (15 birds / m ) . A 90 chicks 7days old were distributed into two treatments (45 chicks per treatment ) , T1 chicks fed a diet withoutaspirin (control) , chicks in T2 fed a diet supplemented with 0.2 % aspirin (2 mg aspirin / kg feed) .Each treatment subdivided into three replicates and each replicate reared in 1 X 1 m pen . The dataobtained revealed the following:Blood serum glucose concentration were 189 mg /100 ml , cholesterol concentration were 207mg /100 ml , uric acid concentration were 4.6 mg /100 mll of T1 chicks at 7 weeks of age , adding 0.2% of aspirin to the diet (T2) decreased blood serum glucose , cholesterol and uric acid of broiler chicksto 178 , 182 and 4.2 mg /100 ml respectively , in the same time adding aspirin to the diet increasedsignificantly (P<0.01) total protein concentration which were 5.1 gm /100 ml compaired with 4.6gm/100 ml of control chicks (T1) at 7 weeks of age . Adding aspirin to the diet increased significantly(P<0.01) blood serum phosphorus and calcium concentrations which indicated the importance ofadding aspirin in broiler diet to rebalance blood biochemical parameters and reducing crowding stressduring high density production

Report on Antidiabetic, Diuretic and Analgesic Activities of Methanolic Extract of Leaves of Strychnos colubrina L.an Endangered Medicinal Plant

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Indira Priyadarsini A. ◽  
S K M Basha2 ◽  
Chakrapani I S ◽  
Nagalakshmi Devamma M
Keyword(s):  
Blood Glucose ◽  
Medicinal Plant ◽  
Analgesic Activity ◽  
Methanolic Extract ◽  
Tail Flick ◽  
Methanolic Extracts ◽  
Endangered Medicinal Plant ◽  
Sugar Levels ◽  
Analgesic Activities ◽  
Dose Dependent

Strychnos colubrina L. belongs to family Loganiaceae was known as snake wood tree.The methanolic extracts from Strychnos colubrina L. leave collected from different provinces in Penchalakona of Nellore district were prepared by decoction and maceration with methanol and evaluated for their antidiabetic, diuretic and analgesic activities. Methanolic extracts from Strychnos colubrina L. leaves (SCM) were evaluated for anti diabetic effect in Streptozocin (STZ) induced diabetes in rats. The blood sugar levels were analysed as indices of diabetes. 200 mg/kg b.w. of the extract showed a greater reduction in blood glucose level which was comparable to glibenclamide. To find out diuretic efficacy, SCM of leaves were administered to experimental rats orally at doses of 100 and 200 mg/kg and compared with Furosemide (20 mg/kg,o.p) as the standard. The rats treated with SCM of leaves in a dose of 200 mg/kg shown near similar urine output and electrolytes excretion when compared to the respective control. The analgesic activity of SCM of leaves is estimated using tail flick in mice. Results demonstrated that SCM of leaves exhibited a potent dose-dependent analgesic activity in all tested models for analgesia. This report could be used for medicinal and pharmaceutical exploration in the future.

scholarly journals Cytotoxic Effects of Bangladeshi Medicinal Plant Extracts

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Shaikh J. Uddin ◽  
I. Darren Grice ◽  
Evelin Tiralongo
Keyword(s):  
Medicinal Plant ◽  
Cell Lines ◽  
Cancer Cell ◽  
Plant Extracts ◽  
Aqueous Extracts ◽  
Healthy Mouse ◽  
Selective Cytotoxicity ◽  
Mouse Fibroblasts ◽  
Methanolic Extracts ◽  
Medicinal Plant Extracts

To investigate the cytotoxic effect of some Bangladeshi medicinal plant extracts, 16 Bangladeshi medicinal plants were successively extracted withn-hexane, dichloromethane, methanol and water. The methanolic and aqueous extracts were screened for cytotoxic activity against healthy mouse fibroblasts (NIH3T3) and three human cancer-cell lines (gastric: AGS; colon: HT-29; and breast: MDA-MB-435S) using the MTT assay. Two methanolic extracts (Hygrophila auriculataandHibiscus tiliaceous) and one aqueous extract (Limnophila indica) showed no toxicity against healthy mouse fibroblasts, but selective cytotoxicity against breast cancer cells (IC501.1–1.6 mg mL−1). Seven methanolic extracts fromL. indica,Clerodendron inerme,Cynometra ramiflora,Xylocarpus moluccensis,Argemone mexicana,Ammannia bacciferaandAcrostichum aureumand four aqueous extracts fromHygrophila auriculata,Bruguiera gymnorrhiza,X. moluccensisandAegiceras corniculatumshowed low toxicity (IC50> 2.5 mg mL−1) against mouse fibroblasts but selective cytotoxicity (IC500.2–2.3 mg mL−1) against different cancer cell lines. The methanolic extract ofBlumea lacerashowed the highest cytotoxicity (IC500.01–0.08 mg mL−1) against all tested cell lines among all extracts tested in this study. For some of the plants their traditional use as anticancer treatments correlates with the cytotoxic results, whereas for others so far unknown cytotoxic activities were identified.

Sign in / Sign up

Export Citation Format

Share Document