scholarly journals Laboratory diagnosis of the Tropicamide non-drug consumption

2021 ◽  
Vol 10 (4) ◽  
pp. 188-196
Author(s):  
O. Yu. Strelova ◽  
Yu. V. Slustovskaya ◽  
A. N. Grebenyuk
Keyword(s):  
Enzymatic Hydrolysis ◽  
Bioanalytical Methods ◽  
Degradation Products ◽  
Laboratory Diagnosis ◽  
Laboratory Animals ◽  
Laboratory Diagnostics ◽  
Laboratory Practice ◽  
Hair Dye ◽  
Hair Samples ◽  
Validation Parameters

Introduction. Lately, medical services have reported a lot of cases caused by taking Tropicamide alone or with other drugs together. Moreover, it has been declared that the increase in the number of resistance cases to Tropicamide consumption has. Due to those facts, Tropicamide was included in the List of Drugs for Medical Use that should be served by the prescriptions in 2015. However, nowadays in Russia there are many combinations of medicines, for instance, Tropicamide and α-adrenergic agonist (phenylephrine) (Midrimax, Fenikamid, Appamide plus) that are not under that regulation. As a result, those medicines are served in pharmacies without any prescriptions. Thus, method developing for Tropicamide determination in the hair samples to establish his consumption period has become a perspective one.Aim. The research aimed to develop a method for the isolation and determination of Tropicamide in the hair samples.Materials and method. Reference standard of Tropicamide was used in this research. The following enzymes – papain, chymopsin, chymotrypsin, and hyaluronidase – were applied in the experiment. To design the long-term consumption of Tropicamide, laboratory animals (Guinea pigs, average masses about 200 – 250 g) with fair and brown nature colour hair were used in this research. The hair of laboratory animals was dyed by professional hair-dye "Estel Professional De Luxe". The following equipment was applied: balance "Sartorius СР224S", pH-meter " FiveEasy ", ball mill Retsch MM-200. The hair samples extracts were analyzed by gas chromatography with mass selective detection (Gas chromatograph model 7890А with mass selective detector model 5977 and MassHunter GC/MS software by Agilent Technologies).Results and discussion. All developed methods of enzymatic hydrolysis (by papain, chymopsin, chymotrypsin, and hyaluronidase) revealed comparable results for the Tropicamide determination in the hair samples. The research showed that the amount of the analyte isolated from the pigmented hair was a bit higher in comparison with the other hair samples (fair hair), despite the melanin gives chemical steadiness property to hair stuff. Moreover, the amount of Tropicamide extracted from the dyed hair samples increased by 30 %. The degradation products of the analyte of interest were not found in the extracts obtained for the dyed hair samples. Thus, the colorant does not destroy the xenobiotic during the hair dying procedure and does not impact the enzymatic hydrolysis process. The values of the validation parameters (precision and accuracy) met the required criteria for bioanalytical methods. Therefore, the enzymatic hydrolysis method can be recommended for application in laboratory practice.Conclusion. In the course of the study, a method for laboratory diagnostics of non-drug use of tropicamide was developed, the reproducibility of which meets the acceptance criteria for bioanalytical methods, which makes it possible to recommend it for work in laboratory practice.

RISTOMYCIN (AGGRISTIN) PRECIPITATION TEST: A NEW, RAPID AND RELIABLE METHOD FOR THE DETECTION OF INTRAVASCULAR CLOTTING

1987 ◽  
Author(s):  
G Pfliegler ◽  
J Arnout ◽  
J Vermylen
Keyword(s):  
Reliable Method ◽  
Degradation Products ◽  
Laboratory Diagnosis ◽  
Blood Collection ◽  
Vein Thrombosis ◽  
Fibrin Degradation Products ◽  
Acid Citrate Dextrose ◽  
Citrate Dextrose ◽  
Precipitation Test

The rapid and specific detection of fibrin monomers (fm) and fibrin degradation products (fdp) is of major importance in the laboratory diagnosis of disseminated intravascular coagulation, deep vein thrombosis or pulmonary embolism. Most methods in use are either time-consuming, needing special techniques, or insensitive and poorly specific. Some time ago, Watanabe and Tullis described a simple and rapid, semiquantitative test to detect fm and fdp in plasma, based on the finding that ristocetin in low concentrations (1.0-1.5 mg/ml) can specifically precipitate fm and fdp. To 0.4 ml ACD plasma, 0.1 ml ristocetin (2.5 mg/ml) is added and vortexed. The mixture is then incubated for 30 min at 20°C and centrifuged at 50xg for 5 min. The test is considered to be positive when fibrin-like strands or small or large pellets are observed on the bottom of the tube. More recently, Pfliegler et al. reported that ristomycin (AGGRISTIN), a structural analogue of ristocetin, can replace ristocetin in this test.Here we report on further results with the ristomycin (AGGRISTIN) precipitation test in 138 patients with various intravascular thrombotic events. The results of this test, performed on ACD plasma, were compared to the serum fdp values detected by immunoelectrophoresis (IEF) and by the haemagglutina-tion inhibition test (HIT). In all 30 cases with serum fdp above 30 ug/ml (HIT) or 28 pg/ml (IEF), the precipitation test was positive; at lower fdp concentrations, as detected by HIT or IEF, the test still was positive in 70 per cent of these thrombosis patients, suggesting a superior sensitivity. In 16 patients with elevated fibrinogen levels (but no evidence of thrombosis), the test was positive in only 3. No false positive results were detected in 16 healthy controls. Preliminary results show that the minor disadvantage of the test (blood collection on acid citrate dextrose) may readily be overcome by the in vitro adjustment of the pH of citrate plasma, commonly used for other haemostatic tests, to between 7.0 and 7.4.On the basis of our results we suggest that the AGGRISTIN (ristomycin) precipitation test is a simple, rapid and reliable method for the laboratory diagnosis of intravascular clotting.

Possibilities of cytological diagnostics in gynecology. Human papillomavirus as an interdisciplinary problem

2022 ◽  
pp. 15-21
Author(s):  
Oksana Anatolievna Gizinger ◽  
◽  
Irina Yurievna Lepina ◽  
Marina Nikolaevna Bagdasaryan ◽  
◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Screening Test ◽  
Molecular Genetic ◽  
Laboratory Diagnosis ◽  
Diagnostic Methods ◽  
Laboratory Diagnostics ◽  
Cytological Examination ◽  
Stage Of Development ◽  
Current Stage ◽  
Cytological Diagnostics

The article presents an analysis of current information on the etiology, pathogenesis, laboratory diagnosis of human papillomavirus. It is shown that at the current stage of development of laboratory diagnostics there is a reliable screening test — cytological examination of smears taken from the ecto- and endocervix. To diagnose HPV, a combination of microscopic (cytological studies) and molecular genetic (PCR) diagnostic methods is used.

scholarly journals Confronting the Moscow Healthcare System with the Shocks of COVID-19

2021 ◽  
Vol 2 (1) ◽  
pp. 41-46
Author(s):  
Aleksandr N. Tsibin ◽  
Munira F. Latypova ◽  
Olga I. Ivanushkina
Keyword(s):  
Public Health ◽  
Large Scale ◽  
Virus Disease ◽  
Laboratory Diagnosis ◽  
Capital City ◽  
Laboratory Diagnostics ◽  
Successful Implementation ◽  
Laboratory Service ◽  
The Government ◽  
Challenging Situation

Introduction. Transmissible coronavirus SARS-CoV-2I is the seventh known coronavirus that causes an acute infectious disease predominantly affecting the lungs (Corona Virus Disease 2019, COVID-19). The COVID-19 pandemic exposed serious gaps in health systems preparedness. The epidemic urgently required priority organizational measures to contain and reduce the spread of COVID-19. Public health authorities had to make decisions in a challenging situation where there was a lack of knowledge, experience, and great confidence, and the number of infected was steadily increasing. Purpose. The purpose of this article is to present the unique experience of Moscow in organizing a large-scale laboratory examination of the population of a metropolis with about 12.6 million inhabitants to meet the needs of the capital in testing for SARS-CoV-2 virus and combating its circulation in conditions of the COVID-19 pandemic. Materials and Methods. The decisions made and the measures taken by the Government of Moscow, the Moscow Operational Staff, the DZM and the DZM Laboratory Service to slow the growth of the COVID-19 epidemic among the population of the capital are listed step-by-step. Results. In the course of organizational activities, sufficient capacity to maintain the public health infrastructure in terms of laboratory diagnosis of the new coronavirus infection was ensured by the joint efforts. Safe laboratory diagnostics for detecting, treating, and isolating COVID-19 cases and contacts have been established in the capital city. Thanks to the successful implementation of timely decisions, the spread of infection in the city of Moscow has been slowed. The Moscow government has reported a steady decline in cases of the new coronavirus disease and most hospitals have switched to a safe treatment regimen for patients requiring hospitalization. Centralized laboratories with readiness to perform screening and referral studies for COVID-19 outbreaks have been established within the structure of the DZM.

scholarly journals Lymphocytes blast-transformation reaction: modification for allergological practice

2018 ◽  
Vol 17 (2) ◽  
pp. 88-92
Author(s):  
I. V. Manina ◽  
V. Yu. Sergeev ◽  
N. V. Golubtsova ◽  
A. Yu. Sergeev
Keyword(s):  
Video Camera ◽  
Laboratory Diagnostics ◽  
Blast Transformation ◽  
Routine Laboratory ◽  
Laboratory Practice ◽  
Digital Cmos ◽  
Transformation Reaction

Introduction. Lymphocytes blast-transformation reaction (RBLT) is necessary for patient’s inspection with immunologic infringements. It is applied in the different fields of medicine to identification of a sensitization to antigens (allergens).Research objective – to modify and automate RBTL for application in routine laboratory practice.Materials and methods. RBTL, antigens (allergens). Software: Windows 7; Intel Pentium G4500 CPU. Digital CMOS video camera, Mikmed-6 microscope.Results. We analyzed difficulties when using RBTL in laboratory allergological practice. We created program files for automation of RBTL.Conclusion. We adapted and automated RBTL for laboratory diagnostics using.

Concurrent Determination of Daclatasvir and Sofosbuvir in Pure Binary Mixture and Their Combined Film Coated Tablets by a Simple Stability Indicating RP-HPLC Method

2021 ◽  
pp. 5913-5918
Author(s):  
Ramreddy Godela ◽  
Sowjanya G
Keyword(s):  
Degradation Products ◽  
Hplc Method ◽  
Forced Degradation ◽  
Good Resolution ◽  
Stability Indicating ◽  
Extreme Stress ◽  
Rp Hplc ◽  
Highly Sensitive ◽  
Validation Parameters ◽  
Acid And Base

A trouble-free, simple, specific and highly sensitive stability indicating phase HPLC method was developed for concurrent assessment of Daclatasvir and Sofosbuvir in pure and in their combined tablet formulation. An effectual separation was accomplished by using XDB Phenyl (250 x 4.6mm, 5µ,100 A0) column, mobile phase composition of Acetonitrile: buffer(0.1%v/v Trifluoroaceticacid in water) (50:50 v/v) and isocratic elution at a flow rate of 1ml/min and detection wavelength of 275nm. The extreme stress conditions like hydrolysis with acid and base, peroxide oxidation, thermal decomposition were used as per ICH specifications to assess the stability of the analytes in bulk and dosage forms. The retention times of Daclatasvir and Sofosbuvir were found at 2.8 and 3.7min respectively. The proposed method has linear response in the concentration ranges from 12 to 36µg/ml and 80 to 240 µg/ml for Daclatasvir and Sofosbuvir respectively. The detection and quantification limits calculated as 2.5μg/ml and 7.8μg/ml for DCL, 5.2μg/ml and 15.8μg/ml SOF respectively. All the method validation parameters were met the acceptance limits of Q2 specifications of ICH procedures. The degradation products produced by forced degradation studies were have good resolution from Daclatasir and Sofosbuvir peaks, which represents the methods stability. The proposed RP-HPLC method was highly sensitive, precise, stability indicating and economical. That’s why the method has the capacity to employ in the pharmaceutical manufacturing of Daclatasvir and Sofosbuvir and routine analysis in quality control department.

scholarly journals Production of Carbohydrates from Cardoon Pre-Treated by Acid-Catalyzed Steam Explosion and Enzymatic Hydrolysis

Energies ◽  
2019 ◽  
Vol 12 (22) ◽  
pp. 4288 ◽  
Author(s):  
Alessandro Bertini ◽  
Mattia Gelosia ◽  
Gianluca Cavalaglio ◽  
Marco Barbanera ◽  
Tommaso Giannoni ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Lignocellulosic Biomass ◽  
Steam Explosion ◽  
Degradation Products ◽  
Liquid Fraction ◽  
Raw Material ◽  
Total Carbohydrate ◽  
Carbohydrate Degradation ◽  
Acid Catalyzed ◽  
Pre Treatment

Cardoon (Cynara cardunculus) is a promising crop from which to obtain oilseeds and lignocellulosic biomass. Acid-catalyzed steam explosion is a thermochemical process that can efficiently pre-treat lignocellulosic biomass. The drawback is the production of a high number of carbohydrate degradation products in the liquid fraction that could inhibit microbial growth. In this work, the lignocellulosic biomass of cardoon, gathered from a dedicated field, were used as the raw material for the production of fermentable monosaccharides by employing acid-catalyzed steam explosion. The raw material was pre-soaked with a dilute 1% (w/w) sulfuric acid solution and then subjected to steam explosion under three different severity conditions. The recovered slurry was separated into solid and liquid fractions, which were individually characterized to determine total carbohydrate and inhibitor concentrations. The slurry and the washed solid fraction underwent enzymatic hydrolysis to release glucose and pentose monosaccharides. By conducting the pre-treatment at 175 °C for 35 min and hydrolyzing the obtained slurry, a yield of 33.17 g of monosaccharides/100 g of cardoon was achieved. At the same conditions, 4.39 g of inhibitors/100 g of cardoon were produced.

scholarly journals Screening for anti-rubella IgM ad libitum

1983 ◽  
Vol 90 (1) ◽  
pp. 1-5 ◽  
Author(s):  
P. P. Mortimer ◽  
R. S. Tedder
Keyword(s):  
Clinical Medicine ◽  
Laboratory Diagnosis ◽  
The State ◽  
Laboratory Practice ◽  
Ad Libitum

There are few applications of virology to clinical medicine that have received more attention than the laboratory diagnosis of rubella. Many laboratories attempt it and most readers of this journal will be familiar with the techniques that have been used. It would therefore be superfluous to review the ‘state of the artm’ had there not recently been an innovation that is likely to alter laboratory practice in this field.

scholarly journals BIOLOGICAL PROPERTIES OF FUSOBACTERIUM NECROPHORUM ISOLATED FROM INFECTED CATTLE TISSUES IN ALMATY REGION

REPORTS ◽  
2020 ◽  
Vol 5 (333) ◽  
pp. 63-72
Author(s):  
N.P. Ivanov ◽  
◽  
N.N. Egorova ◽  
S.N. Sarimbekova ◽  
V.Yu Sushi ◽  
...  
Keyword(s):  
Causative Agent ◽  
Pure Culture ◽  
Biological Material ◽  
Sampling Site ◽  
Small Ruminants ◽  
Fusobacterium Necrophorum ◽  
Biological Properties ◽  
Laboratory Animals ◽  
Laboratory Research ◽  
Laboratory Diagnostics

Necrobacteriosis (necrobacteriosis) is established as an infectious disease characterized by purulent-necrotic lesions of tissues mainly of the lower parts of the extremities, especially in the area of the corolla, and in some cases in the oral cavity, on the udder, in the genitals, liver, lungs and other tissues and organs. Many animal species are affected by necrobacteriosis. The most susceptible and sensitive to Fusobacterium necrophorum are reindeer, cattle and small ruminants, pigs, and rabbits. A constant carrier of the causative agent of necrobacteriosis in the rumen and intestines of ruminants has been established, it is found in food particles during chewing, in feces, in objects of the external environment. The disease is especially often observed in animals kept in damp places with poor zoohygienic conditions. Infection of animals occurs when the pathogen enters the injured skin areas or when the mucous membranes are macerated. The work was carried out in the laboratory and production conditions of KazSRVI LLC and at the MTF of the Arkabay settlement of the Talgar district of the Almaty region, where stall keeping of animals is practiced. Material for laboratory research (sections from the horny tissue of the hoof on the border with the healthy one) were taken fresh and inoculated on a nutrient medium for anaerobes (Kitt-Tarozzi medium). Samples of the selected biological material were plated on Kitt-Tarozzi medium at the sampling site on the farm. The biological material taken from sick animals was examined within several hours after taking in accordance with the guidelines for laboratory diagnostics of necrobacteriosis (YEAR INDICATION METHOD). To get rid of the numerous accompanying microflora and obtain a pure culture of F. necrophorum, a bioassay was set up on laboratory animals - rabbits. On the 14-15th day after infection, the experimental rabbits died, which is evidence of the high pathogenicity of the isolated cultures. A pure culture of F. necrophorum from rabbit’s internal organs, not contaminated with extraneous microflora, was cultured. It was found that rabbits are the optimal biomodel for purification of the F. necrophorum culture. The results of cultivation of the causative agent of presented necrobacteriosis on solid and liquid nutrient media are. The biochemical properties of the isolated cultures have been studied. It was found that epizootic cultures of the causative agent of necrobacteriosis in cattle emitted hydrogen sulfide, formed ammonia, and had hemolytic properties. In experiments in vitro and in vivo, it was found that the isolated cultures of F. necrophorum showed hyaluronidase activity. Cultures of F. necrophorum had high catalase activity, i.e. split hydrogen peroxide with the release of gas bubbles. Four cultures of F. necrophorum, isolated from biological material from cattle, were identical in biological properties.

scholarly journals COVID-19 clinical and laboratory diagnosis overview

2021 ◽  
Vol 96 (1) ◽  
Author(s):  
Rania A. Zayed ◽  
Dalia Omran ◽  
Abeer A. Zayed
Keyword(s):  
False Negative ◽  
Clinical Decision ◽  
Laboratory Diagnosis ◽  
Clinical Suspicion ◽  
Laboratory Diagnostics ◽  
Main Body ◽  
Rt Pcr ◽  
Serological Tests ◽  
Negative Results ◽  
Important Approach

Abstract Background COVID-19 was identified in Wuhan, China, in December 2019, and rapidly spread worldwide, being declared global pandemic on the 11th of March 2020. Since its emergence, COVID-19 has raised global concerns associated with drastic measures that were never adopted in any previous outbreak, to contain the situation as early as possible. Main body The 2019 novel corona virus (2019-nCoV) or SARS-CoV-2 is the causative agent of COVID-19. 2019-nCoV genetic sequence was rapidly identified within few days since the first reported cases and RT-PCR kits became available for COVID-19 diagnosis. However, RT-PCR diagnosis carries a risk of false-negative results; therefore, additional serologic tests are needed. In this review, we summarize the clinical scenario that raises suspicion of COVID-19 and available laboratory diagnostics. Conclusion The most important approach in the battle against COVID-19 is rapid diagnosis of suspicious cases, timely therapeutic intervention and isolation to avoid community spread. Diagnosis depends mainly on PCR testing and serological tests. However, even in the context of negative lab test results and clinical suspicion of COVID-19 infection, clinical decision should be based on clinical suspicion.

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