scholarly journals Chemical assessment, antioxidant and antimicrobial of leafs extracts of Virola sebifera, an Amazonian medicinal plant

2021 ◽  
Vol 10 (15) ◽  
pp. e578101523068
Author(s):  
Claudiane Lima Ribeiro ◽  
Rayele Moraes Silva ◽  
Rachel de Moura Nunes Fernandes ◽  
Mirelle Ribeiro Araújo ◽  
Ilsamar Mendes Soares ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antimicrobial Properties ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Amazon Forest ◽  
Important Species ◽  
E Coli ◽  
Soxhlet Apparatus ◽  
Ultrasound Bath ◽  
Promising Source

The high biodiversity of Amazon forest implies in a great number of plants with ethnopharmacological utilization. V. sebifera is one of the most important species of Virola genus, used in treatment of rheumatism, arthritis, dyspepsia, malaria, muscle pain and erysipelas. This study aimed to investigate its chemical composition, antioxidant and antimicrobial properties. For this, leafs extracts i) 70% ethanol in ultrasound bath (CEU); ii) 70% ethanol in Soxhlet (CES); and iii) sequential extraction in Soxhlet apparatus, starting with hexane (HE), followed by methanol (ME), and 70% ethanol extract (EE). Phenolic concentration, total flavonoid and antioxidant activity were assessed. The highest phenolic and total flavonoid contents were found in CEU and EE showed the best antioxidant activity. The most relevant substances identified by GC-MS analysis were the Kusunokinin, Hinokinin and catechol, among others first time related in V. sebifera. The antimicrobial activity was tested against Staphylococcus aureus, S. epidermidis, Salmonella typhimurium, Escherichia coli, and Candida albicans. The CEU, CES, EM, and EE obtained positive results against S. aureus and S. epidermidis. CES and EM also inhibited S. typhimurium and E. coli. Based on these results, V. sebifera can be recognized as a promising source of antioxidant and antimicrobial compounds.

scholarly journals In vitro antibacterial and antioxidant activity of Rosmarinus officinalis

2021 ◽  
Vol 285 ◽  
pp. 05012
Author(s):  
A. K. Milyuhinа ◽  
L. A. Zabodalova ◽  
U. Kyzdarbek ◽  
I. R. Romazyaeva ◽  
N. Yu. Klyuchko
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Ethanol Extract ◽  
Rosmarinus Officinalis ◽  
E Coli ◽  
Rosmarinus Officinalis L ◽  
Antibacterial And Antioxidant Activity

Rosmarinus officinalis L. is known for its antioxidant and antibacterial properties against a variety of microorganisms. In this work, research has been carried out on pharmacy rosemary. Its antioxidant and antimicrobial properties have been determined. This study showed that the ethanol extract of R. officinalis L. has a pronounced antimicrobial activity against strains of both gram-positive and gram-negative bacteria. The extract also showed good antioxidant activity. Rosemary showed significantly more potent antibacterial activity against E. coli. The diameter of the zone of oppression was 17 mm. Thus, Rosemary Pharmaceutical has demonstrated potential as a dietary supplement due to its stronger antibacterial activity.

scholarly journals Analysis of antioxidant activity, total phenolic and flavonoid contents of ethanol extract of Litsea cubeba Lour. Bark

2021 ◽  
Vol 332 ◽  
pp. 08005
Author(s):  
Aminah Dalimunthe ◽  
Dewi Pertiwi ◽  
Mahatir Muhammad ◽  
Denny Satria
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanol Extract ◽  
The Body ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Litsea Cubeba ◽  
Ferric Reducing Antioxidant Power ◽  
Phenolic And Flavonoid

Free radicals also play a role in the pathology of various degenerative diseases such as cancer, rheumatism, coronary heart disease, cataracts, and others. Free radicals can come from within the body (endogenous) and outside the body (exogenous). Litsea cubeba (Lour,) is a Lauraceae family plant which have contents volatile oils which used as antimicrobial, anticancer on breast cancer, pesticide, antideppressants, antiinflammation, antioxidant, and neuro pharmacology. The extract was prepared using water with the soxhletation method. The antioxidant activity was determined with the 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-Azinobis [3-ethylbenzothiazoline-6)-sulfonic acid] -diammonium salt (ABTS) and Ferric Reducing Antioxidant Power (FRAP) methods. Total flavonoid and total phenolic content were determined with colorimetric methods. Antioxidant activity measured as IC50 was 23.37 ± 0.42 µg/mL; 111.21 ± 0.42 and 109.01 ± 0.28 respectively. The extract was found to contain high levels of total phenolic (282.93 ± 0.33 mg GAE/g) and total flavonoid 7.49 ± 0.51 mg QE/g). The results reveal that ethanol extract of Litsea cubeba Lour. Bark has antioxidant potential. The further analysis is to isolation antioxidant compound.

scholarly journals EVALUATION OF INVITRO ANTI-OXIDANT ACTIVITY OF MAGNOLIA CHAMPACA

2021 ◽  
Vol 6 (8) ◽  
pp. 73-77
Author(s):  
G.SAI SRUTHI ◽  
K. SPANDANA ◽  
RAMANJANEYULU K ◽  
HIMABINDHU J
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Soxhlet Apparatus ◽  
Dpph Method ◽  
Anti Oxidant

The aim of this article is to evaluate antioxidant activity of leaf extract of Magnolia champaca by using in vitro assay. Extraction was carried out with ethanol by using Soxhlet apparatus. The invitro antioxidant activity of ethanol extract has been investigated by 1, 1-diphenyl, 2-picryl–hydrazyl free radical (DPPH) method. The ethanol extract exhibited maximum antioxidant activity. The results have been compared with the standard ascorbic acid.

scholarly journals THE QUALITY PARAMETERS, TOTAL FLAVONOIDS DETERMINATION AND ANTIOXIDANT ACTIVITY COMPOUND OF ANDALIMAN FRUIT ANDALIMAN FRUIT (ZANTHOXYLUM ACANTHOPODIUM DC.) EXTRACT

2021 ◽  
pp. 34-40
Author(s):  
YUNAHARA FARIDA ◽  
WIDI AZELA ◽  
MEGA ELOK LESTAR ◽  
DIAH KARTIKA PRATAMI
Keyword(s):  
Antioxidant Activity ◽  
Methyl Palmitate ◽  
Ethanol Extract ◽  
Quality Parameters ◽  
Total Flavonoid ◽  
Gas Chromatography Mass Spectrometry ◽  
Uv Spectrophotometry ◽  
Benzenedicarboxylic Acid ◽  
Ic50 Value ◽  
Dpph Method

Objective: The aim of this study was to investigate the chemical compound of the antioxidant activity of the extract from andaliman fruit (Zanthoxylum acanthopodium DC.) and the quality parameters and the total flavonoid determination of the highest antioxidant activity. Methods: The andaliman fruits were extracted using a solvent such as n-hexane, ethyl acetate and 70% ethanol obtained extracts, follow the antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The quality parameters and total flavonoid determination were performed from highest antioxidant activity. Fractionated was done using vacuum liquid chromatography (VLC) with the mobile phase chloroform-methanol (9:1 ~1:9). Purification with Thin Layer Chromatography (TLC) preparative, identification using UV-spectrophotometry, FTIR, Gas chromatography Mass Spectrometry (GCMS) analysis. Results: The 70% of ethanol has the highest antioxidant activity with IC50 value 84.11±0.47 ppm; the quality parameters in this study meet the quality requirements, both specific and nonspecific parameters. Total flavonoid content of the 70% ethanol extract was 2.37±0.02%. The results of the GCMS studies showed the presence of 1,2-benzenedicarboxylic acid, mono (2-ethylhexyl) ester; squalene, methyl palmitate, methyl isopalmitate, n-tetracosanol-1 and 4-isopropyl chalcone. Conclusion: The results indicate that the 70% ethanol extracts of andaliman fruits contains 1,2-benzenedicarboxylic acid, mono (2-ethylhexyl) ester; squalene, methyl palmitate, methyl isopalmitate, n-tetracosanol-1 and 4-isopropyl chalcone have antioxidant activity with IC50 value 51.35 ppm.

scholarly journals Antimicrobial, antioxidant activities and toxicity on Cavia porcellus of Dialium angolense Welw. Ex Oliv, a traditional medicinal plant from Bagira in Eastern of DR Congo

2020 ◽  
Vol 13 (2) ◽  
pp. 166-180
Author(s):  
Bashige Chiribagula V ◽  
Bakari Amuri S ◽  
Okusa Ndjolo Philippe ◽  
Kahumba Byanga J ◽  
Duez P ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Acute Toxicity ◽  
Antioxidant Activities ◽  
Antimicrobial Properties ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Cavia Porcellus ◽  
E Coli

Dialium angolense is used in Bagira for its various medicinal properties particularly in the management of infectious diseases. In this study, the methanol and aqueous extracts of leaves and fruits were evaluated for their in vitro antioxidant and antimicrobial properties and their in vivo toxicity on Cavia porcellus. The major phytochemical classes of extracts were screened using standard in-tube reactions. The antimicrobial study was tested on Candida albicans, Escherichia coli, Salmonella typhi, Staphylococcus aureus and Streptococcus pneumoniae using agar well diffusion and dilution methods, while the antioxidant activity was evaluated by a DPPH assay. For the acute toxicity study, animals (6/group) were orally given in a single dose 5000, 1000 or 15000 mg of extract/kg body weight (BW) then observed for 14 days. In sub-acute toxicity assays, 150 or 300 mg/kg BW/day were orally given, and animals observed for 28 days. Total phenolics and total flavonoids contents ranged 1.19 to 1.61 mg GAE.g-1 and 0.45 to 1.01 mg QEg-1, respectively. The extracts presented antioxidant activity with IC50 ranging 4.9 to 6.9 µg/mL. The minimal inhibitory concentration (MIC) on tested strains ranged from 1.9 to 500 µg/mL with the aqueous extract of fruits as a most active extract: MIC=1.9 µg/mL on E. coli and C. albicans. No signs of toxicity were noted during the acute and sub-acute toxicity assessments, suggesting a maximal tolerate doses (MDT) and LD50 > 15000 mg/kg BW. This study highlights the antioxidant and antimicrobial activities of Dialium angolense and suggests that further studies be directed towards the isolation of active compounds.

scholarly journals ANTIBACTERIAL ACTIVITY OF Samanea saman LEAF ETHANOL EXTRACT AGAINST Escherichia coli AND Staphylococcus aureus AND ITS TOTAL FLAVONOID AND PHENOLIC CONTENTS

Jurnal Kimia ◽  
2018 ◽  
pp. 121
Author(s):  
W. S. Rita ◽  
I M. D. Swantara ◽  
I. A. R. Astiti Asih ◽  
N.K. Sinarsih
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
E Coli ◽  
Phenolic Contents ◽  
Agar Disc ◽  
Samanea Saman ◽  
And Staphylococcus Aureus

Antibacterial activity of Samanea saman usually shows a positif correlation to the flavonoid and phenolic contents.  The aim of this study was to evaluate the antibacterial activity of the ethanol extract of Samanea saman against Escherechia coli and Staphylococcus aureus and determine the total flavonoid and phenolic contents of the extract. The extraction was done by ethanol 96% at room temperature. The antibacterial assay was conducted by agar disc diffusion method. The total flavonoid and phenolic contents were determined by UV-Vis Spectrofotometer with the standard of quersetin and galic acid, respectively. The extraction of 250 g of Samanea saman leaves resulted in 24.5 g of ethanol extracts. The ethanol extract showed a moderate inhibition of 8.33 mm towards E. coli and a strong inhibition of 13.6 mm towards S. aureus at the concentration of 4%. The Minimum Inhibitory Concentration (MIC) of the extract against E. coli and S.aureus were of 3% and 0.3%”, respectively. The total flavonoid and phenolic contents were successively 1233.2991 mg QE/100g and 2544.6154 mg GAE/100g. Keywords: Escherechia coli, flavonoid and phenolic content,  Samanea saman, Staphylococcus aureus

scholarly journals Valorization of Agri-Food Waste from Pistachio Hard Shells: Extraction of Polyphenols as Natural Antioxidants

Resources ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 45
Author(s):  
Nunzio Cardullo ◽  
Melania Leanza ◽  
Vera Muccilli ◽  
Corrado Tringali
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Industrial Processing ◽  
Phenolic Constituents ◽  
Potential Applications ◽  
Hard Shell ◽  
Promising Source ◽  
By Products

The agricultural processing industry usually generates a remarkable amount of by-products rich in bioactive compounds, which can be exploited for agri-food or nutraceutical applications. Pistachio’s hard shell is one of the major by-products from pistachio industrial processing. The aim of this work was the evaluation of pistachio shells as a potential source of natural antioxidants. We evaluated different extraction procedures by measuring total phenolic content, total flavonoid content and antioxidative activity (DPPH•, TEAC and ORAC). The microwave-assisted ethanol extract turned out to be the most promising and was fractionated by XAD-16 column chromatography, affording six fractions analyzed through HPLC/ESI-MS/MS and 1H-NMR to identify the main antioxidative constituents. Fractions Fr4–Fr6 demonstrated the highest antioxidant activity. Gallic acid and a monogalloylglusose isomer are the main phenolic constituents of Fr4. Both simple and complex phenolics, such as flavonoids and hydrolysable tannins, were identified in fractions Fr5 and Fr6; pentagalloylglucose and kaempferol, well-known for their antioxidant activity, are the most abundant constituents. The results highlighted that the proposed methodology can be an effective way to recover bioactive phenolic compounds from pistachio hard shell, making this by-product a promising source of compounds with potential applications in food and healthcare sectors.

scholarly journals Penentuan Kadar Flavonoid Total dan Uji Antioksidan Ekstrak Etanol Daun Kasturi (Mangifera casturi Kosterm.) dengan Metode DPPH

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Alifni Adha Bakti ◽  
Liling Triyasmono ◽  
Muhammad Ikhwan Rizki
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Natural Resources ◽  
Experimental Research ◽  
Ethanol Extract ◽  
Total Flavonoid ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Ic50 Value ◽  
Anti Oxidant

ABSTRAK Bahan alam dapat dijadikan bahan obat baru karena mengadung metabolit sekunder. Di Indonesia terdapat lebih kurang 30.000 jenis tumbuh-tumbuhan yang mengandung metabolit sekunder, lebih kurang 7.500 jenis diantaranya termasuk tanaman berkhasiat obat. Salah satunya adalah tanaman kasturi (Mangifera casturi Kosterm.). Tanaman M. casturi merupakan tumbuhan khas Kalimantan Selatan yang hanya dimanfaatkan oleh masyarakat untuk dikonsumsi, tidak untuk pengobatan. Penelitian ini bertujuan untuk menentukan kadar flavonoid total dan aktivitas antioksidan dari ekstrak etanol daun M. casturi. Penelitian ini bersifat non-eksperimental. Sampel yang digunakan adalah daun M. casturi yang berasal dari Kabupaten Banjar, Kalimantan Selatan. Penentuan Kadar flavonoid total dilakukan secara spektrofotometri UV-Vis dengan pereaksi kompleks AlCl3 sedangkan aktivitas antioksidan ditentukan dengan metode DPPH (2,2-difenil-1-pikrilhidrazil). Hasil penelitian diperoleh kadar flavonoid total ekstrak etanol daun M. casturi sebesar 9,31 ± 0,08 %b/b dan aktivitas antioksidan dengan nilai IC50 sebesar 34,558 ppm sehingga termasuk dalam kategori antioksidan yang sangat aktif. Kata kunci: Antioksidan, DPPH, Flavonoid Total, Mangifera casturi. ABSTRACT Natural resources can be used as the new medicine ingridients because it has second metabolite. In Indonesia, there are more than 30.000 kinds of plants that contain second metabolite, more or less than 7.500 kinds of those are medicinal plants. One of those plants is Kasturi (Mangifera casturi Kosterm.). M. casturi is a typical plant from South Kalimantan that be used as a food not as a medicine. The purpose of this research is to determine the total of flavonoid content and anti-oxidant activity from ethanol extract of M. casturi leaves. This study is a non-experimental research. Sample which used in this research is M. casturi Leaves from Banjar Region, South Kalimantan. The research for total Flavonoid content is done by UV-Vis spectrophotometric with AlCl3 reagent complex while the anti-oxidant activity is determined by DPPH (2,2-difenil-1-pikrilhidrazil) method. The result of this research are extract of M. casturi leaves obtains 9,31 ± 0,08 %b/b of total flavonoid and the antioxidant activity result with IC50 value is 34,558 ppm, so it can be categorized as a very active anti-oxidant. Keywords: Antioxidant, DPPH, Total Flavonoid, Mangifera casturi.

scholarly journals Determination of Total Phenolic, Total Flavonoid and Antioxidant Activity of Karamunting Leaves (Rhodomyrtus tomentosa (Aiton) Hassk) Extract and Fraction

2021 ◽  
Vol 9 (2) ◽  
pp. 72-78
Author(s):  
Rahma Dona
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Total Flavonoid ◽  
Hexane Fraction ◽  
Total Phenolic ◽  
Water Fraction ◽  
Ic50 Value

Karamunting (Rhodomyrtus tomentosa (Aiton) Hassk) is a family of Myrtaceae which has been used by the community as traditional medicine. The purpose of this research is determine total phenolic and total flavonoid level and test antioxidant activity of Karamunting Leaves. Determination of total phenolic using gallic acid standard by the Folin Ciocalteu method. The results obtained total phenolic in water fraction of 99 mgGAE/g, ethanol extract 94,1 mgGAE/g, ethyl acetate fraction 83,3 mgGAE/g and n-hexane fraction 41,4 mgGAE/g. Determination of total flavonoid using standard quersetin with AlCL3 complex formation method, the results obtained total flavonoid in water fraction of 156,8 mgQE/g, ethanol extract 192,6 mgQE/g, ethyl acetate fraction 89,4 mgQE/g and n-hexane fraction 31,3 mgQE/g. Determination of antioxidant activity was determined by DPPH radical capture test (1,1-diphenyl-2-picrylhydrazyl), the results obtained in water fraction with IC50 value 15,02 µg/mL, ethanol extract 14,06 µg/mL, ethyl acetate fraction 14,48 µg/mL with very strong activity of antioxidant, whereas in the n-hexane fraction was categorized as weak antioxidant activity with an IC50 value of 264,02 µg/mL.

Sign in / Sign up

Export Citation Format

Share Document