scholarly journals Pengaruh Ekstrak Etanolik Atau Ekstrak Dekok Ganoderma Lucidum (ß Glucan) Terhadap Hormon Testosteron, Sel Leidiq Testis Rattus Norvegicus Jantan.

2019 ◽  
Vol 5 (1) ◽  
pp. 13-23
Author(s):  
Bambang Wasito Tjipto
Keyword(s):  
Rattus Norvegicus ◽  
Ganoderma Lucidum ◽  
Aging Process ◽  
Late Onset ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Total Testosterone ◽  
Anabolic Hormone ◽  
Testosterone Levels

The process of "aging" causes a decline in physical and mental strength. In general, more and more bodily organs are complained of by elderly people, and suffer from chrnic diseases. Older men experience various changes including a decrease in endocrine, cardiovascular, neurological, eye, skin, reproductive and so on. The Late Onset Hypogonadism (LOH) is a syndrome that decreases sexual, physical, and psychological abilities associated with a decrease in testosterone levels in the blood. In middle age, the age of 45-59 years begins to decrease body function, including decreased reproductive function and the function of sexuality, hormones such as testosterone. which is known as aging process. Testosterone is an anabolic hormone. Giving Ganoderma Lucidum in a preliminary study of normal one-year-old male Rattus norvegicus rats, water extract (dekok) and ethanol extract with different doses, 10 mg / kg, 20 mg / kg bw, and 30 mg kg / bb proved to increase total testosterone hormone serum significantly at a dose of 30 mg / kg bw, then researchers increase the dose to 50 mg / kg bw. Purpose of the study: Analyzing the effect of dekok extract or Ganoderma lucidum ethanolic extract on the hormone testosterone, testicular Leidiq cells that are undergoing the aging process. Results: Test profile of testosterone, after administration between controls, extract of dekok and male ethanol extract of 18 months old male Leydig Rattus norvegicus testis. In different tests on testosterone, there were significant differences between controls, dekok extract and ethanol extract. The results of testosterone levels in ethanol extract were higher. Conclusion: Dekok and Ganoderm lucidum ethanol extract can increase testosterone hormone

Serum total testosterone levels in a patient with late onset 21-hydroxylase deficiency and a twin gestation

2007 ◽  
Vol 87 (5) ◽  
pp. 1212.e5-1212.e8 ◽  
Author(s):  
Lindsay M. Mains ◽  
Ruth B. Lathi ◽  
Richard O. Burney ◽  
Michael H. Dahan
Keyword(s):  
Late Onset ◽  
Total Testosterone ◽  
Hydroxylase Deficiency ◽  
Testosterone Levels ◽  
21 Hydroxylase Deficiency

scholarly journals Uji Efektivitas Kombinasi Ekstrak Etanol Jamur Kayu (Ganoderma lucidum) dengan Daun Sirsak (Annona muricata) terhadap Tikus Putih Galur WIstar (Rattus norvegicus) Hiperglikemia (Effectivity Test of Wood Mushroom (Ganoderma lucidum) with Soursop Leaves (Annona muricata) Ethanol Extract Combination to Hyperglicemic Wistar Strain White Mouse (Rattus norvegicus)

2020 ◽  
Vol 10 (1) ◽  
pp. 14
Author(s):  
Jesica Christy Naibaho ◽  
Rooije Rumende ◽  
Hanny Pontororing
Keyword(s):  
Rattus Norvegicus ◽  
Ganoderma Lucidum ◽  
Ethanol Extract ◽  
Pancreatic Disease ◽  
White Mouse ◽  
Annona Muricata ◽  
Treatment Groups ◽  
Wistar Strain ◽  
Friedman's Test ◽  
Ideal Number

Uji Efektivitas Kombinasi Ekstrak Etanol Jamur Kayu (Ganoderma lucidum) dengan Daun Sirsak (Annona muricata) terhadap Tikus Putih Galur WIstar (Rattus norvegicus) Hiperglikemia(Effectivity Test of Wood Mushroom (Ganoderma lucidum) with Soursop Leaves (Annona muricata) Ethanol Extract Combination to Hyperglicemic Wistar Strain White Mouse (Rattus norvegicus) Jesica Christy Naibaho*, Rooije R.H. Rumende, Hanny PontororingProgram Studi Biologi FMIPA Universitas Sam RatulangiJl. Kampus Unsrat, Manado 95115*Email korespondensi: [email protected], (Article History: Received 31-12-2019; Revised 15-01-2020; Accepted 24-01-2020) Abstrak Diabetes merupakan penyakit dengan indikasi pankreas tidak dapat memproduksi hormon insulin dalam jumlah cukup ataupun ketidakmampuan tubuh mempergunakan produksi insulin dengan baik. Penelitian ini bertujuan untuk menguji efektifitas dua konsentrasi ekstrak kombinasi jamur kayu (G. lucidum) dengan daun sirsak (A. muricata) dibandingkan dengan obat diabetes jenis glibenklamid.  Penelitian menggunakan metode eksperimental toksisitas akut dengan lima kelompok perlakuan dan lima ulangan. Analisis data yang digunakan adalah uji Friedman dengan uji lanjut Mann-Whitney. Hasil penelitian menunjukkan jumlah konsentrasi ideal kombinasi ekstrak jamur kayu (G. lucidum) dengan daun sirsak (A. muricata) yang dapat diinduksi terhadap tikus galur wistar hiperglikemia adalah 150 mg/kgBB dan 300 mg/kgBB tikus. Kombinasi ekstrak berpengaruh terhadap kontrol gula darah tikus galur wistar. Konsentrasi optimum kombinasi ekstrak di antara kedua dosis uji ekstrak adalah 300 mg/kgBB. Dosis 300 mg/kgBB kombinasi ekstrak dibandingkan dengan glibenklamid.Kata Kunci: Diabetes, pankreas,hormon insulin,glibenklamid   Abstract Diabetes is a pancreatic disease that can not produce enough insulin hormone or body’s inability to employ insulin hormones properly.The purpose of this study was to tested the effectiveness of two concentrations of wood mushrooms (G. lucidum) with soursop leaves (A. muricata) extract compared to glibenclamide as one of diabetes remedy. Research used acute toxicity experimental methods with five treatment groups and five repetitions. Data were analyzed using Friedman’s test and Mann-Whitney’s test. The results showed that the ideal number of wood mushrooms (G. lucidum) with soursop leaves (A. muricata) extract which can induced to wistar hyperglycemic mouse were 150 mg/kgBM and 300 mg/kgBM. Extract combination has an effect on mice glucose control. Optimum concentration of Extract Combination between the two extract test doses was 300 mg/kgBM. 300 mg/kgBM dose of wood mushrooms (G. lucidum) and soursop leaves (A. muricata)  extract avowed more effective than glibenclamide.Key words: Diabetes, pancreas, insulin hormone, glibenklamide

scholarly journals Edible mycorrhizal species Lactarius controversus Pers. 1800 as a source of antioxidant and cytotoxic agents

2016 ◽  
Vol 70 (2) ◽  
pp. 113-122 ◽  
Author(s):  
Aleksandra Novakovic ◽  
Maja Karaman ◽  
Ivan Milovanovic ◽  
Miona Belovic ◽  
Milena Raseta ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Water Extract ◽  
Chemical Characterization ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Cytotoxic Agents ◽  
Breast Cancer Cell Lines ◽  
Cytotoxic Activities ◽  
Mushroom Species ◽  
Reducing Ability

The aim of this work was to study chemical profile and antioxidant and cytotoxic activities of ethanol and water extracts of Lactarius controversus (Pers.) 1800 mushroom species growing in eastern Serbia. The chemical characterization of phenolic compounds performed by HPLC-MS/MS demonstrated the presence of quinic acid among others. Determination of antioxidant activity, including radical scavenging effects on DPPH?, NO?, OH? and SOA radicals and ferric reducing ability was investigated. The highest DPPH radical scavenging effect was obtained for water extract (LcAq) while ethanol extract (LcEtOH) demonstrated the highest FRAP activity. Hexane extract applied in antibacterial assay against three pathogenic strains demonstrated antibacterial effect only against S. aureus ATCC25922. Anti-proliferative properties against estrogen dependent MCF 7 breast cancer cell lines using MTT showed higher activity for ethanolic extract.

scholarly journals AKTIVITAS ANTIOKSIDAN DAN IDENTIFIKASI SENYAWA EKSTRAK JAMUR LINGZHI (Ganoderma lucidum) DENGAN LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY (LC-MS)

EKOLOGIA ◽  
2019 ◽  
Vol 19 (2) ◽  
pp. 65-72
Author(s):  
Farida Nuraeni ◽  
Septi Bernadetha Br Sembiring
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Time Variation ◽  
Ganoderma Lucidum ◽  
Water Extract ◽  
Ethanol Extract ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Dpph Method

Lingzhi mushroom is widely used as an alternative treatment to reduce blood pressure and blood sugar levels, to maximize the potential of the Lingzhi fungus antioxidant tests are carried out. This study aims to determine the potential antioxidant activity of water extract and 70% ethanol extract of Lingzhi mushroom with maceration extraction time variation and identification of compounds with Liquid Chromatography Mass Spectrometry (LC-MS). This research begins with the determination of fresh lingzhi mushrooms then made simplicia and extracted by maceration with time variation with soaking time of 24 hours, 48 hours and 72 hours each with 2 solvents namely water and 70% ethanol. The extract was tested by phytochemistry followed by testing the antioxidant activity of Lingzhi mushroom extract (ganoderma lucidum) by DPPH method. Then the compounds were identified by Liquid Chromatography-Mass Spectrometry (LC-MS). Based on the results of research that maceration for 1 hour (1 day) Lingzhi mushroom with 70% ethanol extraction has the potential as an antioxidant with an IC50 value of 94.83 ppm. The results of identification with LC-MS in 70% ethanol extract as a compound that has potential as an antioxidant are Bisphenol M compounds, and 1- - [[2- (3,4-Dimethoxyphenyl) amino]} -3-methyl-2-octylpyrido [1 , 2-a] benzimidazole-4-carbonitrile.

Resource Utilization Residue of Pleurotus ostreatus

2013 ◽  
Vol 807-809 ◽  
pp. 2048-2053
Author(s):  
Yong Xiong ◽  
Chun Yan Zhao ◽  
Yi Jian Chen ◽  
Wan Song Yue
Keyword(s):  
Pleurotus Ostreatus ◽  
Ganoderma Lucidum ◽  
Water Extract ◽  
Ethanol Extract ◽  
Flammulina Velutipes ◽  
Edible Fungi ◽  
Mycelium Growth ◽  
Extract Concentration ◽  
Ethanol Extracts ◽  
Better Than

The allelopathy of spent substrate extracts including water extract and ethanol extract ofPleurotus ostreatuson the mycelium growth of six edible fungi, i.e.Flammulina velutipes,Ganoderma lucidum Karst,Pleurotus ostreatusandCordyceps (three species), were investigated using Petri dishes approach. The results indicated that the spent substrate extracts have different effects on their mycelium growth. The mycelium ofFlammulina velutipesgrows better than control check with increasing water extract concentration. The mycelia ofGanoderma lucidum Karstgrow first promotion after inhibition with increasing water extract concentration. The mycelia ofFlammulina velutipes, Ganoderma lucidum Karst, Pleurotus ostreatusare promoted by ethanol extracts. The mycelia of Cordyceps (three species) grow first promotion after inhibition with ethanol extracts. The results can provide reference values for rational utilization of the spent mushroom substrate.

scholarly journals Uji Efektivitas Kombinasi Ekstrak Etanol Jamur Kayu (Ganoderma lucidum) dengan Daun Sirsak (Annona muricata) terhadap Tikus Putih Galur WIstar (Rattus norvegicus) Hiperglikemia

2020 ◽  
Vol 10 (1) ◽  
pp. 13
Author(s):  
Jesica Christy Naibaho ◽  
Rooije R.H. Rumende ◽  
Hanny Pontororing
Keyword(s):  
Rattus Norvegicus ◽  
Ganoderma Lucidum ◽  
Ethanol Extract ◽  
Pancreatic Disease ◽  
White Mouse ◽  
Annona Muricata ◽  
Treatment Groups ◽  
Wistar Strain ◽  
Friedman's Test ◽  
Ideal Number

Uji Efektivitas Kombinasi Ekstrak Etanol Jamur Kayu (Ganoderma lucidum) dengan Daun Sirsak (Annona muricata) terhadap Tikus Putih Galur WIstar (Rattus norvegicus) Hiperglikemia(Effectivity Test of Wood Mushroom (Ganoderma lucidum) with Soursop Leaves (Annona muricata) Ethanol Extract Combination to Hyperglicemic Wistar Strain White Mouse (Rattus norvegicus) Jesica Christy Naibaho*, Rooije R.H. Rumende, Hanny PontororingProgram Studi Biologi FMIPA Universitas Sam RatulangiJl. Kampus Unsrat, Manado 95115*Email korespondensi: [email protected], (Article History: Received 31-12-2019; Revised 15-01-2020; Accepted 24-01-2020) Abstrak Diabetes merupakan penyakit dengan indikasi pankreas tidak dapat memproduksi hormon insulin dalam jumlah cukup ataupun ketidakmampuan tubuh mempergunakan produksi insulin dengan baik. Penelitian ini bertujuan untuk menguji efektifitas dua konsentrasi ekstrak kombinasi jamur kayu (G. lucidum) dengan daun sirsak (A. muricata) dibandingkan dengan obat diabetes jenis glibenklamid.  Penelitian menggunakan metode eksperimental toksisitas akut dengan lima kelompok perlakuan dan lima ulangan. Analisis data yang digunakan adalah uji Friedman dengan uji lanjut Mann-Whitney. Hasil penelitian menunjukkan jumlah konsentrasi ideal kombinasi ekstrak jamur kayu (G. lucidum) dengan daun sirsak (A. muricata) yang dapat diinduksi terhadap tikus galur wistar hiperglikemia adalah 150 mg/kgBB dan 300 mg/kgBB tikus. Kombinasi ekstrak berpengaruh terhadap kontrol gula darah tikus galur wistar. Konsentrasi optimum kombinasi ekstrak di antara kedua dosis uji ekstrak adalah 300 mg/kgBB. Dosis 300 mg/kgBB kombinasi ekstrak dibandingkan dengan glibenklamid.Kata Kunci: Diabetes, pankreas, hormon insulin, glibenklamid   Abstract Diabetes is a pancreatic disease that can not produce enough insulin hormone or body’s inability to employ insulin hormones properly.The purpose of this study was to tested the effectiveness of two concentrations of wood mushrooms (G. lucidum) with soursop leaves (A. muricata) extract compared to glibenclamide as one of diabetes remedy. Research used acute toxicity experimental methods with five treatment groups and five repetitions. Data were analyzed using Friedman’s test and Mann-Whitney’s test. The results showed that the ideal number of wood mushrooms (G. lucidum) with soursop leaves (A. muricata) extract which can induced to wistar hyperglycemic mouse were 150 mg/kgBM and 300 mg/kgBM. Extract combination has an effect on mice glucose control. Optimum concentration of Extract Combination between the two extract test doses was 300 mg/kgBM. 300 mg/kgBM dose of wood mushrooms (G. lucidum) and soursop leaves (A. muricata)  extract avowed more effective than glibenclamide.Key words: Diabetes, pancreas, insulin hormone, glibenklamide

Uji Aktivitas Antijamur Ekstrak Gambir (UncariagambirRoxb) terhadap Candida albicans secara in Vitro

2015 ◽  
Vol 5 (2) ◽  
pp. 62
Author(s):  
Suraini Suraini ◽  
Chairani Chairani ◽  
Enlita Enlita
Keyword(s):  
Candida Albicans ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Dilution Method ◽  
Antifungal Effect ◽  
Ofcandida Albicans ◽  
Close Relationship ◽  
Tube Dilution Method

Effective treatment is being sought for the discovery of antifungal compounds.One of Plants that are suspected as a potential antifungal is Gambir (Uncaria gambierRoxb.). Gambir contains high antioxidants. Gambier containing catechin, katekutannat, quercetin, gallic acid, and catechol elagat acid, has been studied to determine its antifungal activity. This study was done by using experimental in vitro tube dilution method. The general objective of this study was to investigate the inhibition of ethanolic extract and water extract of gambier towards the growth of Candida albicans. Specific objectives were to determine Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC)of ethanolic extract and water extract of gambier towards the growth of Candida albicans. The concentration used were 50% , 60% , 70% , 80% , 90% and 100% . The results showed thatMICof the ethanolic extract of gambier could not be determined, while the MBCwas 100%. One Way ANOVA statistical analysis results showed p= 0.004 ( p< 0.05 ), indicating significant differences in changes of the concentration of ethanol extract gambier to the number of colonies ofCandida albicans. Correlation test showed a close relationship between the concentration of gambier extract and the number of colonies ( r = -9.900 : p < 0.05 ). Regression test yielda linear regression equation Y = 5,034-0,048x with R Square coefficient ( r2 ) of 0.802. Based on the research results it can be concluded that the ethanolic extract of gambier has antifungal effect against Candida albicans.

scholarly journals Analisis Metabolit Sekunder dan Aktivitas Ekstrak Etanol Kulit Buah Salak (Salacca zalacca (Gaertn.) Voss) Terhadap Kadar Glukosa dan Ureum Kreatinin Tikus Putih Jantan (Rattus norvegicus)

KOVALEN ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 161-168
Author(s):  
Tien Wahyu Handayani ◽  
Agustinus Widodo ◽  
Risna Yanti ◽  
Erdy Prasetyo ◽  
Zulfaidah ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Rattus Norvegicus ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Phytochemical Screening ◽  
Fruit Peel ◽  
Treatment Groups ◽  
White Rats

The snake fruit peel (Salacca zalacca (Gaertn.) Voss) traditionally has several health benefits, one of which is to treat diabetes mellitus. This study aimed to determine the content of secondary metabolites in the ethanolic extract of snake fruit peel and its activity on glucose and urea creatinine levels in male white rats (Rattus norvegicus). Phytochemical screening of extracts was carried out qualitatively and quantitatively using UV-Vis Spectrophotometry. This study is a laboratory experimental study using 30 test animals divided into 6 treatment groups (normal control, negative control, positive control, 70 mg/kg BW, 140 mg/kg BW, and 280 mg/kg BW). The results showed that the ethanol extract of the bark of the salak fruit contained 7.61 %w/w alkaloids, flavonoids 0.041% w/w, tannins 1.18% w/w, and saponins 2% w/w. Ethanol extract of salak fruit peel dose of 140 mg/kg BW affected decreasing blood glucose and urea creatinine levels. The skin of the salak fruit has the potential to be further investigated as an antidiabetic.

scholarly journals Diagnose the Bioactive Compounds in Flaxseed Extract and Its Oil and Use Their Mixture as an Antioxidant

2020 ◽  
Vol 33 (1) ◽  
pp. 172-188
Author(s):  
Wasan K. A. Al-Temimi ◽  
Najla H.S. Al- Garory ◽  
Ali A. Khalaf
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Flaxseed Oil ◽  
Deionized Water ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Flaxseed Extract

The aim of this study is to extract the important antioxidants components of flaxseed in different ways. The Determination of total phenol content, antioxidant activity and percentage of linoleic acid inhibition by DPPH for the components (Lignan, oil, 80 and 100% ethanolic extract and the deionized water extract). The components of flaxseed oil were identified using GC/MS. The efficiency of the mixture (80% ethanolic extract and oil)  was determined by inhibiting the process of oxidation of linoleic acid during storage periods (0, 7, 14 and 21 days) at laboratory temperature, and the following results were obtained:  The yield of lignan, oil, ethanol extract (80 and 100)% and deionized  water were (0.12,40.52,10.9,9.6 and 13 )g.100g-1 seed respectively, while the total phenolic content of the above components was (700,1165,3315,2098 and 483)mgGAE.100g-1,respectively. Flaxseed oil gave the highest antioxidant activity 79.3% with an inhibition percentage 73.19%, compared with ethanolic extract, lignan and water extract. The diagnosis of GC/MS flaxseed oil showed that  the oil was contained in compounds with antioxidant activity, including mono, di and tri-terpens such as Copaene, Monoterpene, ?- Sesquiphellandrene, Squalene, diethyl phthalate ,?-Sitosterol and Hexadecanoic acid, as well as, the presence of ascorbic acid and gamma tocopherol with good concentrations (8.68 and 2.62)% respectively. The mixture of 80% ethanol and oil extract showed an effect in decreasing of peroxide values with an increase of concentration of the added mixture (0.1, 0.4 and 0.10 mg.mg-1). The maximum reduction of the peroxide value at the concentration was 0.6 mg. mg-1, compared with the standard sample during the different durations of storage.

scholarly journals Effectiveness of Sargassum sp. ethanolic extract on traumatic ulcers healing in the labial mucosa of Wistar strain (Rattus norvegicus)

2019 ◽  
Vol 31 (1) ◽  
pp. 73
Author(s):  
Annisya Ristie Arwidasari ◽  
Twi Agnita Cevanti ◽  
Isidora Karsini Soewondo
Keyword(s):  
Wound Healing ◽  
Rattus Norvegicus ◽  
Economic Value ◽  
Methyl Cellulose ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Test Results ◽  
Wistar Strain ◽  
Acid Gel ◽  
Iron And Calcium

Introduction: Oral ulcer lesions are one of the most common lesions in the oral cavity. The prevalence of traumatic ulcers is quite high compared to other oral lesions. Brown algae, Sargassum sp. is one of the algae contains flavonoids, vitamin A, vitamin C, iron, and calcium, which play a role in wound healing and has economic value. This study was aimed to determine the effectiveness of Sargassum sp. ethanolic extract on traumatic ulcers healing in the labial mucosa of Wistar strain (Rattus norvegicus). Methods: 28 Wistar strain (Rattus norvegicus) weighed 150-200 grams. The samples were divided into 4 groups (K1, K2, P1, P2); each group consisted of 7 mice. K1 group was administered with Hydroxy Propyl Methyl Cellulose (HPMC), K2 was administered with 0.2% hyaluronic acid gel, P1 was administered with 50% Sargassum sp. extract gel, and P2 was administered with 75% Sargassum sp. extract gel. In each group, the ulcer was made on the mice labial mucosa; then the ulcer diameter was measured and observed on day 1 and day 7. Results: The mean diameter ± standard deviation of traumatic ulcer calculations were K1 (1.09 ± 0.40), K2 (1.81 ± 0.67), P1 (1.39 ± 0.43) and P2 (2.16 ± 0.49) respectively. One way ANOVA test results showed significant differences in K1, K2, P1, and P2 groups. Conclusion: Ethanol extract of Sargassum sp. is effective in accelerating the traumatic ulcers healing at the concentration of 75%.Keywords: Traumatic ulcer, Sargassum sp., wound healing.

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