EFEKTIVITAS STIMULANSIA KOMBINASI SARI UMBI BIT DAN BUAH APEL TERHADAP KETAHANAN FISIK DAN KADAR Hb TIKUS PUTIH Sprague Dawley

EKOLOGIA ◽  
2020 ◽  
Vol 19 (1) ◽  
pp. 20-26
Author(s):  
Moerfiah . ◽  
Yulianita . ◽  
Deni Setiawan
Keyword(s):  
Physical Exercise ◽  
Effective Dose ◽  
Apple Juice ◽  
Positive Control ◽  
Negative Control ◽  
Sprague Dawley ◽  
Endurance Test ◽  
Experimental Animals ◽  
Treatment Groups ◽  
White Rats

Human physical exercise is influenced by hemoglobin levels in the blood, stimulant testing of underlying fatigue should be tested for Hb levels. This study aims to determine the increase in stimulation and hemoglobin levels of white rats using beetroot and apple juice. The method used for stimulant testing was the endurance test using a rotarod and for measuring Hb levels using the sahli method with a hemometer. The data observed were the duration of resistance on rotarod stick which were rotated, the experimental animals used were 25 animals divided into 5 treatment groups with each group of 5 rats namely: group of beetroot and apple (1: 1) (2.8g: 0.63mg / 200gBB), comparison (2: 1) of (5.6g: 0.63mg / 200gBB), comparison (1: 2) of (2.8g: 1.26mg / 200gBB), negative control (-) (CMC-Na 0.5%) and positive  control (+) caffeine 20 mg / 200g BB. Dosage treatment is carried out in one day.The results showed that the combination of beetroot and apple juice had an increase in stimulant and hemoglobin levels of white rats with the most effective dose for stimulant enhancement and Hb levels were found in the second dose compared to beetroot and apple juice (2: 1) 5.6 g / 200gBB: 0.63 g / 200gBB.

scholarly journals Formula of Yellow Kepok Banana (Musa acuminata x Musa balbisiana) Corm Extracts As Antiinflamation

2020 ◽  
Vol 30 (2) ◽  
pp. 100
Author(s):  
Desy Muliana Wenas ◽  
Lisana Sidqi Aliya ◽  
Wisma Merry Anjani
Keyword(s):  
Wound Healing ◽  
Positive Control ◽  
Negative Control ◽  
Inflammatory Activity ◽  
Sprague Dawley ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
White Rats ◽  
Anti Inflammatory ◽  
Post Hoc

<p><em><em>Bananas have many types and benefits. One of them is the Yellow Kepok Banana. Banana stem, leaf midrib, and corm contain phytochemicals compound as medicinal properties. The study aimed to determine the effect of the yellow kepok banana corm extract formula as an anti-inflammatory. Banana corm was cut into 0.3 x 1 x 3 cm pieces, dried in the oven with temperature 60 °C for 6 hours, then made 40 mesh powder. The powder extracted using 70 % ethanol by maceration technique for 24 hours, repeated three times. Anti-inflammatory testing was carried out on male </em>Sprague Dawley<em> white rats. A total of 24 white rats were divided into six treatment groups, consists of </em><em>different concentrations of the extract (10, 15, and 20 %), positive control (commercial ointment), negative control, and a normal control (giving a wound without being given any formula). The experiment was designed as a randomized complete, with four replications, data were analyzed using ANOVA and the </em>Post Hoc Games Howell Test<em>. The results showed that the yellow kepok banana extract formula had the potential for wound healing. The 20 % extract formula can accelerate wound healing in a day than the 10 and 15 % formulas. The  speed  is  shown  by  wound  healing  without  scars  from  the   20 %</em> <em>extract formula, which  occurs  earlier  than other formulas. The anti-inflammatory activity of the 20 % formula was comparable to the positive control of commercial ointment. The anti-inflammatory activity of the yellow kepok banana ointment formula is related to the compound of saponins, flavonoids, and tannins. The results indicate that the yellow kepok banana corm extract has the potential to be developed as an anti-inflammatory drug.</em><br /></em></p>

scholarly journals PENGUJIAN EFEKTIVITAS DIURETIK KOMBINASI EKSTRAK ETANOL DAUN AFRIKA (Gymnanthemum amygdalinum Del.) DAN DAUN KELAPA SAWIT (Elaeis guineensis Jacq.) PADA TIKUS JANTAN

2020 ◽  
Vol 3 (1) ◽  
pp. 106-111
Author(s):  
Delisma Simorangkir
Keyword(s):  
Leaf Extract ◽  
Elaeis Guineensis ◽  
Urine Volume ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Treatment Groups ◽  
White Rats ◽  
Group 2 ◽  
Palm Leaf

African Leaf and palm leaf crops have a secondary metabolite content such as alkaloids and flavonoids that are efficacious as diuretics. The purpose of this study is to know the effectiveness of diuretics from the combination  African leaf ethanol extract and palm leaf ethanol extract. Methods used Eksperiemental. The test of diuretic activity is conducted divided into 6 treatment groups. Each group consists of 3 tails of white rats. Group 1 (positive control) administered Furosemide 3.6 mg/kgBB, Group 2 (negative control) was given suspension Na-CMC 0.5%, group 3 without treatment as well as groups of 4.5, and 6 in a row administered a combination of African leaf extract doses and extracts Oil palm leaf ethanol in a row of "50 mg/kgBB + 67, 5mg/kgBB"; "100mg/kgBB + 135 mg/kgBB"; and "200 mg/kgBB + 270 mg/kgBB". Each mouse is then given a 20ml NaCl/kgBB as an oral loading dose. Measured urine volume is recorded every hour for 6 hours after it continues to measure urine volume. The results showed that the combination of African leaf ethanol extract dosage and palm leaf ethanol extract had a diuretic effect if it was compared with negative control. The conclusion of the study was the results of the study showed that the combination of African leaf extract dosage and palm leaf extract is the most effective dose of Group 6 (African leaf extract 200 mg/kgBB + palm leaves 270 mg/kgBB ). Because it produces the highest urine volume of 23.01 ml.

scholarly journals Aktivitas Ekstrak Etanol Kulit Batang Kayu Jawa (Lannea coromandelica) terhadap Penyembuhan Luka Sayat pada Tikus Putih (Rattus Norvegicus L.)

2018 ◽  
Vol 4 (2) ◽  
pp. 113-118
Author(s):  
Umi Calsum ◽  
Akhmad Khumaidi ◽  
Khildah Khaerati
Keyword(s):  
Effective Dose ◽  
Rattus Norvegicus ◽  
Povidone Iodine ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Healing Time ◽  
White Rats ◽  
Lannea Coromandelica ◽  
Healing Wound

Jawa bark (Lannea coromandelica) is a part of the plant that can be used empirically for handling wounds. This study aims to determine the activity of ethanol extract of Jawa bark on healing wound and determine the effective dose compared with povidone iodine. The test animals used were white rats (Rattus norvegicus L.) consisting of 5 groups, namely negative control (vaseline without extract), positive control (povidone iodine), dose of 250 mg/kg BW, dose of 500 mg/kg BW, dose of 750 mg/kg BW. Each rat made an incision in the area parallel to the spine with 2 cm long and 2 mm deep. Measuring the length of the wound was done every day for 14 days. The data obtained were analyzed statistically using One Way Anova and followed by Post Hoc Duncan test. Statistical results showed that the ethanol extract of Jawa bark has an activity in curing slice where the effective dose is a dose of 500 mg/kg BW with a wound healing time of 12 days.

scholarly journals The bone strengthening effects of propolis in ovariectomized female white rats as models for postmenopause

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Dian Ayu Juwita ◽  
Almahdy Almahdy ◽  
Rahmad Abdillah ◽  
Fiony Syahputri
Keyword(s):  
Bone Strength ◽  
Testing Machine ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Impact Testing ◽  
Control Group ◽  
Test Results ◽  
Treatment Groups ◽  
White Rats

Abstract Osteoporosis is a bone disease characterized by decreased quality and strength of bones so that it becomes porous and fracture. Propolis is known to have many pharmacological activity, including an anti-osteoporosis effect. This study aims to determine the effect of propolis administration and the effects of propolis dosage variation in preventing osteoporosis based on the strength value of femur bone impact in female white rats in the form of an ovariectomy postmenopausal model. The rats were divided into 5 groups: positive control group (subjected to ovariectomy), negative control group (not subjected to ovariectomy, and treatment groups that were subjected to ovariectomy and given propolis at a dose of 180 mg/kg BW, dose 360 mg/kg BW and dose 720 mg/kg BW. Propolis was administered orally for 30 days. Bone impact strength testing was undertaken after 30 days using an impact testing machine. Research data were analyzed via one-way ANOVA and continued with the Duncan’s Multiple Range Test. From the test results, we noted that propolis administration had an effect on the value of bone strength, with the dose of 720 mg/kg BW and 360 mg/kg BW having a significant effect, compared with others. With an increase in dose, propolis can provide an increase in the value of bone strength in rat bones.

scholarly journals Utilization of Honey Apis dorsata as Antiosteoporosis on Requirements of Bone Calcium Ash Density on Ovariohysterectomized White Rat (Ratus norvegicus)

2017 ◽  
Vol 3 (6) ◽  
pp. 627 ◽  
Author(s):  
Muhammad Huda Ramadhan Ibrahim ◽  
Abdullah Hasib Hasib ◽  
Siti Nur Rohmah ◽  
Salsabilla Abani ◽  
Samsi Yordan ◽  
...  
Keyword(s):  
Lumbar Vertebrae ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Ash Content ◽  
Control Group ◽  
Apis Dorsata ◽  
Treatment Groups ◽  
White Rats ◽  
Bone Calcium

The purpose of this study was to determine the effect of honey bee Apis dorsata as anti-osteoporosis in calcium ash density (CAD) of bone in osteoporotic-induced rats. The target of this study was to know bone calcium levels after being given honey bees Apis dorsata. In this study, 35 female white rats (Ratus norvegicus) mature was used with weight 200gr. Divided into 5 groups, 2 control groups and 3 treatment groups. The negative control group (SH) was not induced by osteoporosis and was given the only aquadest of 1.5 ml/day. Whereas the positive control group was induced by osteoporosis (OH)  and was given only aquadest 1.5 ml/day. T1, T2 and T3 treatment groups were given bee honey with various doses including 1g / kg ad 1.5 ml aquadest, 2g / kg BB ad 1.5 ml aquadest and 3g / kg BB ad 1.5 ml aquadest. Then after 12 weeks, white rats were sacrificed for lumbar vertebrae. Furthermore, the sixth lumbar spine os vertebrae will be examined for calcium bone ash content. The data were obtained was analyzed using statistical analysis of variance (ANOVA) for the results of the calcium ash content data Keywords: Honey; Osteoporosis; CAD

scholarly journals UJI EFEKTIVITAS SALEP EKSTRAK DAUN JARAK PAGAR (Jatropha curcas L.) TERHADAP PENYEMBUHAN LUKA SAYAT PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus)

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 284
Author(s):  
Repatri A Bawotong ◽  
Edwin De Queljoe ◽  
Deby A Mpila
Keyword(s):  
Wound Healing ◽  
Jatropha Curcas ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Steroid Glycoside ◽  
Treatment Groups ◽  
Jatropha Curcas L ◽  
White Rats ◽  
Wistar Strain

ABSTRACTJatropha leaves (Jatropha curcas L.) contains alkaloid, saponin, tannin, terpenoid, steroid, glycoside, phenol compound and flavonoid through ethanol extract. Until today the community still used the plant as a wound healing medicine. The aim of this study is to examine the effectiveness of jatropha curcas L. leaf extract ointment for healing in male white rats wistar strain.  Jatropha leaves extract ointment was tested on 5 which consists of 3 rats in each treatment groups, that is base ointment (negative control), ointment betadine (positive control), ointment extract of Jatropha leaves 10%, 20% and 40%. Observations has made until the 14th day. And data were tested using ANOVA (Analysis of Variants) and continued with LSD (Least Significant Different) test.  Statistical test results showed a difference in the duration of wound healing in male white rats wistar strain between the ointment extract of jatropha leaf with ointment betadine and vaseline ointment. Keywords: Ointment, Jatropha extract (Jatropha curcas L.), Galur wistar, Wound.ABSTRAK Daun jarak pagar (Jatropha curcas L.) mengandung zat-zat alkaloid, saponin, tannin, terpenoid, steroid, glikosida, senyawa fenol dan flavonoid melalui ekstrak etanol. Hingga saat ini masyarakat masih menggunakan tanaman tersebut sebagai obat penyembuh luka. Penelitian ini bertujuan untuk menguji efektivitas salep ekstrak etanol daun jarak pagar terhadap penyembuhan luka sayat pada tikus putih jantan galur wistar. Salep ekstrak daun jarak pagar  diujikan pada hewan uji tikus sebanyak 5 yang terdiri dari 3 ekor tikus disetiap kelompok perlakuan, yaitu  basis salep (kontrol negatif), salep betadine (kontrol positif), salep ekstrak etanol daun jarak pagar 10%, 20% dan 40%. Pengamatan dilakukan sampai hari ke 14. Data diuji dengan menggunakan ANOVA (Analisis Of Variant) dan dilanjutkan dengan uji LSD (Least Significant Different). Hasil uji statistik menunjukan adanya perbedaan terhadap lamanya penyembuhan luka pada tikus putih jantan galur wistar antara sediaan salep ekstrak daun jarak pagar dengan salep salep betadine dan basis salep. Kata Kunci: Salep, Daun jarak pagar (Jatropha curcas L.), Galur wistar, Luka sayat.

scholarly journals Analisis Metabolit Sekunder dan Aktivitas Ekstrak Etanol Kulit Buah Salak (Salacca zalacca (Gaertn.) Voss) Terhadap Kadar Glukosa dan Ureum Kreatinin Tikus Putih Jantan (Rattus norvegicus)

KOVALEN ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 161-168
Author(s):  
Tien Wahyu Handayani ◽  
Agustinus Widodo ◽  
Risna Yanti ◽  
Erdy Prasetyo ◽  
Zulfaidah ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Rattus Norvegicus ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Phytochemical Screening ◽  
Fruit Peel ◽  
Treatment Groups ◽  
White Rats

The snake fruit peel (Salacca zalacca (Gaertn.) Voss) traditionally has several health benefits, one of which is to treat diabetes mellitus. This study aimed to determine the content of secondary metabolites in the ethanolic extract of snake fruit peel and its activity on glucose and urea creatinine levels in male white rats (Rattus norvegicus). Phytochemical screening of extracts was carried out qualitatively and quantitatively using UV-Vis Spectrophotometry. This study is a laboratory experimental study using 30 test animals divided into 6 treatment groups (normal control, negative control, positive control, 70 mg/kg BW, 140 mg/kg BW, and 280 mg/kg BW). The results showed that the ethanol extract of the bark of the salak fruit contained 7.61 %w/w alkaloids, flavonoids 0.041% w/w, tannins 1.18% w/w, and saponins 2% w/w. Ethanol extract of salak fruit peel dose of 140 mg/kg BW affected decreasing blood glucose and urea creatinine levels. The skin of the salak fruit has the potential to be further investigated as an antidiabetic.

scholarly journals The Influence of Green Tea Infusion (Camellia sinensis) on Renal Histopathological Overview of Male White Rats (Rattus Norvegicus) Sprague Dawley Strain Induced by Ethanol

2020 ◽  
Vol 10 (3-s) ◽  
pp. 111-115
Author(s):  
Muhartono Muhartono ◽  
Katya Rizqita Fitriana
Keyword(s):  
Green Tea ◽  
Camellia Sinensis ◽  
Rattus Norvegicus ◽  
Positive Control ◽  
Negative Control ◽  
Sprague Dawley ◽  
Tea Infusion ◽  
White Rats ◽  
Renal Histopathology ◽  
Keywords Alcohol

Aims: This sudy aims to determine the effect infusion  of green tea (Camellia sinensis) to the renal histopathology picture of male white rats (Rattus norvegicus) Sprague dawley strain ethanol-induced. Methods: This study used 30 rats divided into 5 groups. The negative control (K-) was given distilled water 2 mL/day, the positive control (K +) was given ethanol 2 mL/day, the treatment group (P1, P2, P3) was given ethanol 2 mL and green tea infusion with respective doses of 0.375, 0.75 and 1.5 grams orally for 14 days. Then the renal retrieval is done to be examined microscopically. Results: The mean kidney damage score obtained was K- = 0.36; K + = 3.24; P1 = 2.52; P2 = 2,4; and P3 = 2.2. Data analysis with One Way Anova Test and continued with Pos Hoc and found significant differences in K- to K+, P1, P2, P3; K+ is significant to K-, P1, P2, P3; and P1, P2, P3 are significant to K- & K+. Conclusion: There is an effect of infusion of green tea (Camellia sinensis) on the renal histopathological picture of male white rats (Rattus norvegicus) ethanol-induced Sprague dawley strain. Keywords: alcohol, antioxidant, ethanol, green tea, renal histopathology

scholarly journals AKTIVITAS ANTI-INFLAMASI EKSTRAK ETANOL TEPUNG PELEPAH AREN (Arenga pinnanta)

2020 ◽  
Vol 13 (2) ◽  
Author(s):  
Devy Angreani M ◽  
Meiske Sangi ◽  
Feti Fatimah
Keyword(s):  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Inflammatory Activity ◽  
Control Group ◽  
Treatment Groups ◽  
White Rats ◽  
Wistar Strain ◽  
Anti Inflammatory

Tepung pelepah aren secara tradisional digunakan sebagai obat untuk menghilangkan rasa gatal dan luka bakar pada kulit. Penelitian ini bertujuan untuk menguji aktivitas anti-inflamasi ekstrak etanol tepung pelepah aren (Arenga pinnanta) menggunakan metode induksi karagenan. Ekstrak etanol tepung pelepah aren yang digunakan didapatkan dari hasil maserasi tepung pelepah aren menggunakan etanol hasil redestilasi cap tikus. Metode pengujian aktivitas anti-inflamasi menggunakan 15 ekor tikus putih jantan galur wistar dengan berat 150-200 gram yang dibagi dalam 5 kelompok perlakuan, Kelompok Kontrol negative, Kontrol Positif dan kelompok dosis ekstrak etanol 20%, 25% dan 30%. Hasil penelitian ini menunjukkan kelompok ekstrak etanol tepung pelepah aren dosis 30% memiliki kemampuan inhibisi udem yang sama dengan kontrol positif (Na Diklofenak) dan memiliki kemampuan inhibisi yang lebih besar dibandingkan dosis 20% dan 25%.ABSTRACTPalm stem flour is traditionally used as a remedy for the relief of itching and burns on the skin. This research aims to test the anti-inflammatory activity of palm-flour (Arenga pinnanta) ethanol extract using Caragenan induction method. Palm-dried stem flour extract used from the maceration of palm flour, using ethanol redestilation of rat seals. The method of testing anti-inflammatory activity using 15 male white rats with Wistar strain with a weight of 150-200 grams divided into 5 treatment groups, negative control group, positive control and group dose of ethanol extract 20%, 25% and 30%. The results of this study showed that the group of dried palm flour extract dose 30% have the same inhibition capability with positive control (Na Diklofenak) and have greater inhibition capability compared to the dose 20% and 25%.

scholarly journals THE ROLE OF VITAMIN E (α-TOCOPHEROL) ON TESTOSTERONE LEVEL IN SPRAGUE DAWLEY RATS FOLLOWING CISPLATIN TREATMENT

2019 ◽  
Vol 26 (1) ◽  
Author(s):  
Dian Kartika Rezia ◽  
Lukman Hakim ◽  
Wahjoe Djatisoesanto
Keyword(s):  
Vitamin E ◽  
Adult Male ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Testosterone Levels ◽  
White Rats ◽  
Sprague Dawley Rats

Objective: To observe the difference of testosterone levels in adult male Sprague Dawley rats treated with combination of cisplatin and vitamin E compared to those treated with cisplatin only. Material & Methods: We used 24 adult male Sprague Dawley rats weight 200–300 grams and randomly assigned into 4 groups (n=6). Rats in negative control group (NC) were given intraperitoneal normal saline injection, while the positive control (PC) group were injected with cisplatin 5 mg/kgBW at the end of the 3rd week. Two other groups, P1 and P2, were injected with cisplatin 5 mg/kgBW and given vitamin E orally 50 mg/kgBW and 200 mg/kgBW, respectively. Cardiac blood was aspirated at the end of the 7th week and processed for analysis of testosterone levels. Results: We recorded a significantly lower testosterone levels in rats treated only with cisplatin 5 mg/kgBW (CP) compared to those in CN group (p=0.006), and those receiving combination of cisplatin and vitamin E 50 mg/kgBW (p=0.003) and 200 mg/kgBW (p=0.001). Though not significant, testosterone levels were higher in P2 group than in P1 group (p=0.702). Conclusion: Exposure to cisplatin can lower testosterone levels in white rats, and the administration of vitamin E gives protection against such effect. 

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