scholarly journals Effect of rhizobium isolates and nitrogen on nodulation, growth and yield in lentil

2021 ◽  
Vol 6 (2) ◽  
pp. 5
Author(s):  
Muhammad Tarek ◽  
Rubaiyat Binte Billah ◽  
Saiyera Chowdhury
Keyword(s):  
Adverse Effect ◽  
Biomass Production ◽  
Seed Yield ◽  
Bacterial Isolate ◽  
Growth And Yield ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Straw Yield ◽  
Nodule Number ◽  
Superior Effect

The Experiments were setup in three locations, Magura (23°3´ N 89°2´ E), Rangpur (25°7´ N 89°3´ E) and Mymensingh (24°8´ N 90°0´ E), Bangladesh to know the effect of two bacterial strains and 3 nitrogen doses on nodulation, yield and biomass production. The experimental material consists of one lentil popular variety viz., Binamasur-5 which was inoculated with two promising rhizobial starins/isolates namely LB-21 and LB-40 and three levels of nitrogen viz., 25, 50 and 75 kg N ha-1. The inoculant treatments were un-inoculated control (I0), LB-21 (I1), LB-40 (I2) and mixture of LB-21 and LB-40 (I1+I2). Results indicated that nodule number, nodule weight, straw yield and seed yield at the three locations were greater in bacterial isolates treated plants than in control plants. Results further indicated that mixture of two bacterial isolates had no superior effect against any single bacterial isolate. The two isolates (LB-21 and LB-40) had almost similar greater effect on nodule number, nodule weight, physiological parameters and seed yield with being the highest in LB-40. Application of 25 kg N ha-1 under both bacterial isolates performed the best in seed yield. Application of nitrogen at the rate of 75 kg ha-1 had adverse effect on nodulation, growth and seed yield in lentil. Therefore, for getting higher seed yield in lentil, should apply N as basal dose at the rate of 25 kg N ha-1 and the bacterial isolate, LB-40 may be registered as biofertilizer after few more trials in the different lentil growing areas of Bangladesh.

Physiological attributes of mungbean (Vigna radiata L.) influenced by different sources of nutrients (NPK) in Madhupur tract region of Bangladesh

2021 ◽  
Vol 11 (01) ◽  
pp. 736-748
Author(s):  
M. M. Hossain ◽  
◽  
S. Yesmin ◽  
M. Z. Islam ◽  
M. A. Hossain ◽  
...  
Keyword(s):  
Plant Growth ◽  
Seed Yield ◽  
Block Design ◽  
Growth And Yield ◽  
Higher Plant ◽  
Npk Fertilizers ◽  
Straw Yield ◽  
Combined Application ◽  
Randomized Complete Block Design ◽  
Different Sources

An experiment was conducted at the Sher-e-Bangla Agricultural University farm from April to June 2017 in Kharif season to evaluate the performance of two mungbean varieties under different NPK fertilizers doses in field conditions. The experiment was laid out in Randomized Complete Block Design (RCBD), comprising six treatments with three replications. In the experiment, varieties used were BARI mung-6 and BARI mung-5 and the combination of the treatment were T0 = (control), T1 (10-40-25 NPK kg ha-1), T2 (10-60-45 NPK kg ha-1), T3 (20-50-35 NPK kg ha-1), T4 (30-40-25 NPK kg ha-1) and T5 (30-40-45 NPK kg ha-1), respectively. Mungbean cultivars responded noticeably to the supplementary NPK fertilizers as the crop characters were significantly influenced by different levels of NPK fertilizers. Data were recorded on plant height, leaves plant-1, branches plant-1, pods plant-1, seeds pod-1, 1000-seed weight, seed, and straw yield at different days after sowing. Of the two varieties, BARI mung-6 gave the highest seed yield (1.72 t ha-1) next to the higher plant growth and straw yield On the contrary, BARI mung-5 cultivar produced the lowest seed yield (1.57t ha-1), plant growth, and straw yield. The results showed that T3 (20-50-35 NPK kg ha-1) treatment was the best treatment as regards plant growth and yield parameters. The highest seed yield was produced by treated plot T3 (20-50-35 NPK kg ha-1) over the untreated control plot, T1, T2, T4, and T5, respectively. As for the combined effect, V1T3 (BARI mung-6 and T3 = 20kg N + 50kg P + 35kg K ha-1) performed the best results in all growth and development characters. Therefore, the combined application of 20-50-35 kg NPK ha-1 might be considered to be found optimal to get a considerable seed yield of mungbean variety BARI mung-6.

scholarly journals Potential of carbonic anhydrase and urease bacteria for sequestration of CO2 into aerated concrete

2018 ◽  
Vol 250 ◽  
pp. 03004 ◽  
Author(s):  
A Faisal Alshalif ◽  
JM Irwan ◽  
N Othman ◽  
Adel Al-Gheethi ◽  
Algaifi Hassan ◽  
...  
Keyword(s):  
Carbonic Anhydrase ◽  
Bacterial Isolate ◽  
Cement Kiln ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Jar Test ◽  
Facultative Anaerobic ◽  
Aerated Concrete ◽  
Kiln Dust ◽  
Freeze Dryer

The present study aimed to investigate the potential of bacterial strains from cement kiln dust (CKD) to sequestrate atmospheric CO2 into aerated concrete as a functional for carbonic anhydrase (CA) and urease enzymes. Five samples of CKD was collected from Cement Industries of Malaysia Berhad (CIMA). The most potent bacterial isolates were selected and adapted to grow in 5% of CO2 and in bio-aerated concrete medium. CA enzyme was detected by using a solution of 1.8 g of p-NPA (p-nitrophenyl acetate) and 25 mg of ampicillin at 7-pH. The results of thioglycolate broth medium assay indicated that the bacterial isolates were facultative anaerobic. Furthermore, the results of candle jar test reflected that the bacterial isolates have the ability to survive with 5% of CO2 concentrations. Two bacterial isolates distinctly grow in bio-aerated concrete simulation medium, while only one bacterial isolate was the most potent and has produced in a powder form using freeze dryer to be ready to apply in bio-aerated concrete.

scholarly journals Biological Transformation of Zearalenone by Some Bacterial Isolates Associated with Ruminant and Food Samples

Toxins ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 712
Author(s):  
Sharif Zada ◽  
Sadia Alam ◽  
Samha Al Ayoubi ◽  
Qismat Shakeela ◽  
Sobia Nisa ◽  
...  
Keyword(s):  
Bacterial Isolate ◽  
Carbon Sources ◽  
Preventive Measure ◽  
Food Samples ◽  
Maximum Activity ◽  
Growth Media ◽  
Rrna Gene ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Mrs Broth

Zearalenone (ZEA) is a secondary metabolite produced by Fusarium spp., the filamentous fungi. Food and feed contamination with zearalenone has adverse effects on health and economy. ZEA degradation through microorganisms is providing a promising preventive measure. The current study includes isolation of 47 bacterial strains from 100 different food and rumen samples. Seventeen isolates showed maximum activity of ZEA reduction. A bacterial isolate, RS-5, reduced ZEA concentration up to 78.3% through ELISA analysis and 74.3% as determined through HPLC. Ten of the most efficient strains were further selected for comparison of their biodegradation activity in different conditions such as incubation period, and different growth media. The samples were analyzed after 24 hrs, 48 hrs, and 72 hrs of incubation. De Man Rogosa Sharp (MRS) broth, Tryptic soy broth, and nutrient broth were used as different carbon sources for comparison of activity through ELISA. The mean degradation % ± SD through ELISA and HPLC were 70.77% ± 3.935 and 69.11% ± 2.768, respectively. Optimum reducing activity was detected at 72 hrs of incubation, and MRS broth is a suitable medium. Phylogenetic analysis based on 16S rRNA gene nucleotide sequences confirmed that one of the bacterial isolate RS-5 bacterial isolates with higher mycotoxin degradation is identified as Bacillus subtilis isolated from rumen sample. B05 (FSL-8) bacterial isolate of yogurt belongs to the genus Lactobacillus with 99.66% similarity with Lactobacillus delbrukii. Similarly, three other bacterial isolates, D05, H05 and F04 (FS-17, FSL-2 and FS-20), were found to be the sub-species/strains Pseudomonas gessardii of genus Pseudomonas based on their similarity level of (99.2%, 96% and 96.88%) and positioning in the phylogenetic tree. Promising detoxification results were revealed through GC-MS analysis of RS-5 and FSL-8 activity.

scholarly journals Effect of Bradyrhizobium Inoculation on Nodulation, Biomass Production and Yield of Mungbean

1970 ◽  
Vol 24 (2) ◽  
pp. 95-99
Author(s):  
M Asadul Haque Bhuiyan ◽  
Mosharraf Hossain Mian
Keyword(s):  
Biomass Production ◽  
Seed Yield ◽  
Dry Matter ◽  
Dry Weight ◽  
Nodule Number ◽  
Shoot Dry Weight ◽  
Seed Inoculation ◽  
Matter Production ◽  
Stover Yield ◽  
Shoot Weight

Experiments with or without Bradyrhizobium was carried out with five mungbean varieties at Bangladesh Agricultural University Farm during kharif-I 2001 and kharif-I 2002 seasons to observe nodulation, biomass production and yield of mungbean. Significant influences of the mungbean varieties were observed on nodulation, biomass production and yield. BARI Mung-2 produced the highest nodule number, nodule weight, shoot weight, seed yield (1.03 t/ha in 2001 and 0.78 t/ha in 2002) and stover yield (2.24 t/ha in 2001 and 2.01 t/ha in 2002). Application of Bradyrhizobium inoculant produced significant effect on nodulation, shoot dry weight, seed and stover yields in both trials conducted in two consecutive years. Seed inoculation significantly increased seed (0.98 t/ha in 2001, 27% increase over control and 0.75 t/ha in 2002, 29% increase over control) and stover (2.31 t/ha in 2001 and 2.04 t/ha in 2002) yields of mungbean. Inoculated BARI Mung-2 produced the highest nodulation, dry matter production, seed and stover yields. Considering nodulation, biomass production and seed and yields, BARI Mung-2 was found as the best variety among the five. BARI Mung-5 produced the second highest seed yield followed by BARI Mung-4 and BINA Mung-2, and the lowest seed yield was observed in Barisal local. Keywords: Mungbean, Nodulation, Bradyrhizobium DOI: http://dx.doi.org/10.3329/bjm.v24i2.1251 Bangladesh J Microbiol, Volume 24, Number 2, December 2007, pp 95-99

scholarly journals Integrated application of biochar and bio-fertilizer improves yield and yield components of Cowpea under water-deficient stress

2020 ◽  
Vol 15 (2) ◽  
pp. 94-101
Author(s):  
Seyed Afshin Mousavi ◽  
AliReza Shokuhfar ◽  
Shahram Lak ◽  
Mani Mojaddam ◽  
Mojtaba Alavifazel
Keyword(s):  
Organic Matter ◽  
Biomass Production ◽  
Seed Yield ◽  
Yield Components ◽  
Crop Production ◽  
Growth And Yield ◽  
Yield Production ◽  
Yield And Yield Components ◽  
Phosphorus And Potassium ◽  
Evaporation Pan

A low amount of organic matter and insufficient irrigation are two main challenges facing successful crop production in arid and semiarid regions. Application of biochar as an organic amendment to soil not only can help increase organic matter in soil, but also may alleviate adverse effects of water deficit on plant growth and yield production. To test this hypothesis, a two-year field experiment was conducted to assess the effect of sugarcane biochar on yield and yield components of cowpea in water-deficient soil. Treatments consisted of two levels of seed treatment with nitroxin, three levels of biochar application (0, 4, and 8 ton/ha), and a threelevel irrigation regime (60, 90, and 120 mm from evaporation pan class A), laid out in a split-factorial design. Results showed that the seed number per plant was significantly higher in cowpea when grown with biochar, possibly due to the relief of water-deficient stress and higher phosphorus and potassium content. Biomass production of cowpea declined under a severe waterdeficit condition (ir3) compared to normal irrigation (ir1) in 2018 and 2019, decreasing by 39% and 42%, respectively. The maximum biomass obtained from application of 8 ton/ha biochar reached 617.43 and 664.92 g/m2 in 2018 and 2019, respectively. Seeds treated with nitroxin exhibited 10% and 8% greater biomass production in 2018 and 2019 as compared with control treatments. Seed yield increased with the addition of biochar to soil under all irrigation regimes; however, the maximum seed yield of 266.46 and 275.36 g/m2 was observed when there was no water-deficient stress condition and application of 8 ton/ha biochar in 2018 and 2019, respectively.

scholarly journals Physiological and biochemical responses of soybean (Glycine max L.) to cysteine application under sea salt stress

2019 ◽  
Vol 44 (1) ◽  
Author(s):  
Mervat Sh. Sadak ◽  
Asmaa R. Abd El-Hameid ◽  
Faten S. A. Zaki ◽  
Mona G. Dawood ◽  
Mohamed E. El-Awadi
Keyword(s):  
Adverse Effect ◽  
Superoxide Dismutase ◽  
Salinity Stress ◽  
Seed Yield ◽  
Photosynthetic Pigments ◽  
Saline Water ◽  
Growth And Yield ◽  
Soybean Plant ◽  
Plant Weight ◽  
Beneficial Role

Abstract Background Shortage of water is a major problem facing Egypt; thus, it becomes necessary to use non-traditional sources of water such as saline water in irrigation. Overcoming the adverse effects of saline water and enhancing plant tolerance to salinity stress is the main challenge for increasing plant growth and productivity. Using a natural compound such as cysteine amino acid has an important effect in alleviating the adverse effect of salinity stress on different plant crops. Materials and methods Two pot experiments were carried out during two successive summer seasons to study the beneficial role of cysteine (0, 20, and 40 mg/l) in enhancing growth, some metabolic process, and seed yield quality and quantity of soybean plant grown under salinity stress (0, 3000, and 6000 mg/l). Results Salinity stress (3000 and 6000 mg/l) caused decreases in soybean growth criteria (plant height, number of branches and leaves/plant, dry weight of leaves and stem/plant), photosynthetic pigments and some element contents (nitrogen (N), phosphorus (P), and potassium (K)), and seed yield and yield components (number of pods/plant, weight of pods/plant and number of seeds/plant) as well as oil%. Meanwhile, proline, H2O2 and MDA contents, and superoxide dismutase activity were gradually increased by increasing salinity level. On the other hand, cysteine treatments improved growth and yield of soybean plant either irrigated with tap water or saline water. Cysteine treatments could alleviate the adverse effect of salinity stress on growth and yield of soybean plant through increasing photosynthetic pigments; proline content; N, P, and K contents; superoxide dismutase and catalase activities; and oil% accompanied by decreases in H2O2 and MDA contents as compared with their corresponding controls. In addition, cysteine treatments and/or salinity stress exhibited differences in protein pattern from 112 to 19 kD molecular weight. The appearance of new protein bands reflected the expression of cysteine treatments and salinity stress. Conclusion Cysteine treatments had a beneficial role in alleviating the adverse effect of salinity stress on soybean plant. Forty milligrams per liter of cysteine was the most effective treatment in enhancing salinity tolerance of soybean plant.

scholarly journals Evaluation of New Sesame Varieties for Growth and Yield Performance during the Deyr Season in Afgoye, Somalia

2021 ◽  
pp. 1-7
Author(s):  
Shuaib Abdullahi Siad ◽  
Hassan Nuur Ismaan ◽  
Mohamud Mohamed Isse ◽  
Abdullahi Omar Mohamud ◽  
Mohammad Sohidul Islam ◽  
...  
Keyword(s):  
Seed Yield ◽  
Growth Parameters ◽  
Block Design ◽  
Growth And Yield ◽  
Alternative Methods ◽  
Yield Performance ◽  
Straw Yield ◽  
Local Variety ◽  
Biological Yield ◽  
New Varieties

Aims: The field experiment was conducted to evaluate new sesame varieties for growth and yield performance. Study Design: A randomized completely block design (RCBD) with four replications was used for the experiment. Place and Duration of Study: The study was conducted during the Deyr season (September-November) 2016 at the experimental farm of Somali Agriculture Technical Group (SATG), Afgoye, Somalia. Methodology: Growth and yield characters of the varieties were measured during the trial. A complete of six sesame varieties was used for the trial. The new varieties were Setit, Yemeni, Nigerian, Humera and Indian while a local variety, Dunyar, was used as a control. Results: Results from the experiment revealed that growth parameters such capsule length, number of branches per ∙plant, number of capsules per plant and number of seeds per capsule were significantly different among the varieties. Yield performance of the sesame varieties was also significantly different. It was observed that Humera variety showed the highest seed yield of 2.82 ton per hectare while the Indian variety has given the lowest seed yield of 0.67 ton per hectare. The highest straw yield of 3.45 ton per hectare was recorded for Local variety while the lowest straw yield of 2.76 ton per hectare was observed in Setit variety. Likewise the highest biological yield of 6.18 ton per hectare was observed at Dunyar variety while the lowest biological yield of 3.61 ton per hectare was recorded for Indian variety. Conclusion: The growth and yield of these particular varieties did not outperform the local variety, thus, future studies would be essential to look for other alternative methods and cultivars that would help increase the productivity.

STUDIES ON SOME BACTERIAL DISEASES IN OREOCHROMIS NILOTICUS WITH SPECIAL REFERENCES TO TREATMENT

2018 ◽  
pp. 39-47
Author(s):  
Viola Zaki ◽  
Ahmed EL-gamal ◽  
Yasmin Reyad
Keyword(s):  
Oreochromis Niloticus ◽  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Histopathological Examination ◽  
Klebsiella Oxytoca ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Tissue Samples ◽  
Hydropic Degeneration ◽  
Total Prevalence

he present research carried out to study the common bacterial infections in Oreochromis niloticus (Nile tilapia) in Manzala area at Dakahlia governorate and possible antimicrobial agents used for treatment. A total number of 400 fish were randomly collected from Manzala private farms at Dakahlia governorate and subjected to the clinical, bacteriological and histopathological examination. The highest prevalence of bacterial isolates during the whole period of examination of naturally infected O.niloticus was recorded for A.hydrophila (22.66%), followed by V.alginolyticus (19.01%), V.parahemolyticus (13.80%), Streptococcus spp. (12.24%), A.caviae (11.72%), V.cholera (10.16%), A.salmonicida (7.55%), while the lowest prevalence was recorded for Klebsiella oxytoca (2.86%). The seasonal highest total prevalence of bacterial isolates from examined naturally infected O. niloticus was recorded in spring (30.21%), followed by autumn (28.39%), then summer (22.40%) and the lowest prevalence was recorded in winter (19.01%). Histopathological findings of the tissue samples which collected from different organs of naturally infected O.niloticus revealed that spleen show marked hemosiderosis and sever hemorrhage, gills showsever congestion of lamellar capillaries with marked aneurysm, necrosis and hemorrhage of lamellar epithelium and liver show sever hydropic degeneration and necrosis of hepatocytes, Ciprofloxacin was the most effective antibiotic against all isolated bacterial strains

Biodegradation of organophosphorus Pollutants by Soil Bacteria: Biochemical Aspects and Unsolved Problems

2020 ◽  
Vol 36 (4) ◽  
pp. 126-135
Author(s):  
T.V. Shushkova ◽  
D.O. Epiktetov ◽  
S.V. Tarlachkov ◽  
I.T. Ermakova ◽  
A.A. Leontievskii
Keyword(s):  
Genome Sequencing ◽  
Soil Bacteria ◽  
Activity Regulation ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Russian Science ◽  
Degrading Bacteria ◽  
Phosphorus Source ◽  
Glyphosate Herbicide ◽  
Enzymatic Pathways

The degradation of persistent organophosphorus pollutants have been studied in 6 soil bacterial isolates and in 3 bacterial strains adapted for utilization of glyphosate herbicide (GP) under laboratory conditions. Significant differences in the uptake of organophosphonates were found in taxonomically close strains possessing similar enzymatic pathways of catabolism of these compounds, which indicates the existence of unknown mechanisms of activity regulation of these enzymes. The effect of adaptation for GP utilization as a sole phosphorus source on assimilation rates of several other phosphonates was observed in studied bacteria. The newly found efficient stains provided up to 56% of GP decomposition after application to the soil in the laboratory. The unresolved problems of microbial GP metabolism and the trends for further research on the creation of reliable biologicals capable of decomposing organophosphonates in the environment are discussed. organophosphonates, glyphosate, biodegradation, bioremediation, C-P lyase, phosphonatase, degrading bacteria Investigation of phosphonatase and genome sequencing were supported by Russian Science Foundation Grant no. 18-074-00021.

scholarly journals Effects of Biofertilizer Produced from Bradyrhizobium and Streptomyces griseoflavus on Plant Growth, Nodulation, Nitrogen Fixation, Nutrient Uptake, and Seed Yield of Mung Bean, Cowpea, and Soybean

Agronomy ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 77 ◽  
Author(s):  
Aung Zaw Htwe ◽  
Seinn Moh Moh ◽  
Khin Myat Soe ◽  
Kyi Moe ◽  
Takeo Yamakawa
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Seed Yield ◽  
Mung Bean ◽  
Growth And Yield ◽  
Vegetable Crops ◽  
N Application ◽  
Npk Uptake ◽  
Phosphorus And Potassium ◽  
Leguminous Crops

The use of biofertilizers is important for sustainable agriculture, and the use of nodule bacteria and endophytic actinomycetes is an attractive way to enhance plant growth and yield. This study tested the effects of a biofertilizer produced from Bradyrhizobium strains and Streptomyces griseoflavus on leguminous, cereal, and vegetable crops. Nitrogen fixation was measured using the acetylene reduction assay. Under N-limited or N-supplemented conditions, the biofertilizer significantly promoted the shoot and root growth of mung bean, cowpea, and soybean compared with the control. Therefore, the biofertilizer used in this study was effective in mung bean, cowpea, and soybean regardless of N application. In this study, significant increments in plant growth, nodulation, nitrogen fixation, nitrogen, phosphorus, and potassium (NPK) uptake, and seed yield were found in mung beans and soybeans. Therefore, Bradyrhizobium japonicum SAY3-7 plus Bradyrhizobium elkanii BLY3-8 and Streptomyces griseoflavus are effective bacteria that can be used together as biofertilizer for the production of economically important leguminous crops, especially soybean and mung bean. The biofertilizer produced from Bradyrhizobium and S. griseoflavus P4 will be useful for both soybean and mung bean production.

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