scholarly journals “Lemon grass oil mouth wash- A natural alternative”

2019 ◽  
Author(s):  
Rohit Shah ◽  
Rutuja Donde ◽  
Dipika Mitra
Keyword(s):  
Porphyromonas Gingivalis ◽  
Antibacterial Properties ◽  
Prevotella Intermedia ◽  
In Vitro Test ◽  
Bacterial Strains ◽  
Lemon Grass ◽  
Significant Difference ◽  
Lemongrass Oil ◽  
Mouth Wash

Aim and Objective: The objective of the study was to evaluate the anti-plaque efficacy of lemongrass oil mouthwash. It also assessed the antibacterial properties of lemon grass oil against Prevotella intermedia (Pi), Porphyromonas gingivalis (Pg), and Aggregatibacter actinomycetemcomitans (Aa) in vitro. Materials and Methods: Forty-five periodontally healthy subjects between the age group of 18 and22 years were enrolled in a 4-day plaque re-growth study. Subjects were divided into three groups, i.e., 15 in each group. Mouthwashes were labelled as A (0.25% lemongrass oil mouthwash), B (0.2% Chlorhexidine mouthwash) and C (placebo). Subjects were advised to refrain from any kind of mechanical oral hygiene procedure for four days. Plaque index (PI) was evaluated at baseline and on the fifth day. In vitro testing of lemon grass oil mouthwash was done against strains of Prevotella intermedia, Porphyromonas gingivalis, and Aggregatibactor actinomycetemcomitans, to evaluate the antimicrobial concentration of lemon grass oil mouthwash. Results: PI significantly increased from day 1 to day 5 (P < 0.05) in Groups A, B, and C. In inter-group comparison, there was a statistically significant difference between the three groups. However, chlorhexidine showed superior antiplaque activity. Lemon grass also demonstrated antiplaque activity, however, not as superior as chlorhexidine. In vitro test, lemon grass showed effective inhibition against all three bacterial strains Pi, Pg, and Aa at 0.01% concentration. Conclusion: Lemongrass oil mouthwash potently inhibits plaque formation and hence can be used as a natural herbal alternative. Key Words: lemongrass oil, antiplaque, antibacterial, chlorhexidine.

scholarly journals Rinse Smart: Nutmeg Mouthwash

2019 ◽  
pp. 20-24
Author(s):  
Gaurav Shetty ◽  
Samhita Dabak ◽  
Dipika K. Mitra ◽  
Rohit Shah ◽  
Silvia Rodrigues ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Antibacterial Properties ◽  
Good Alternative ◽  
Prevotella Intermedia ◽  
In Vitro Test ◽  
Age Group ◽  
Growth Study ◽  
Cross Sectional ◽  
Vitro Test

Brief Background This study was undertaken to assess the anti-plaque efficacy of Nutmeg mouthwash. The antibacterial properties of nutmeg mouthwash against Prevotella intermedia (Pi), Porphyromonas gingivalis (Pg), and Aggregatibacter actinomycetemcomitans (Aa) in vitro were also assessed. Materials and Methods In vitro testing of Nutmeg mouthwash against Pi, Pg, and Aa was carried out. Thirty periodontally healthy subjects between the age group of 21- 23 years (mean age 22 years) were enrolled in the cross sectional 4-day plaque re-growth study and were divided into two groups and assigned one mouthwash for each group. Mouthwashes were labelled as A and B. Mouthwash A was a Nutmeg mouthwash; Mouthwash B was commercially available 0.2% Chlorhexidine mouthwash. Subjects were advised to refrain from any kind of mechanical oral hygiene techniques. Plaque index (PI) was checked on day 1 and 5. After a washout period of two weeks, the mouthwashes were switched and cross-over clinical trial was again carried out and plaque scores were taken on day 1 and 5. Results Pi significantly increased from day 1 to day 5 (P < 0.01) in Groups A and B during all the trails. In inter-group comparison, Nutmeg mouthwash was almost comparable to Chlorhexidine mouthwash. However, chlorhexidine showed superior anti-plaque activity. In vitro test, showed Nutmeg mouthwash (0.01%) was effective against Pi, Pg, and Aa. Conclusion: Nutmeg mouthwash was found to be potent plaque inhibitor although less efficacous than Chlorhexidine mouthwash. It can serve as a good alternative for the patients who prefer natural products. Key words Anti-plaque, mouthwash, Nutmeg

scholarly journals Sex-dependent liver cancer xenograft models for predicting clinical data in the evaluation of anticancer drugs

2021 ◽  
Vol 37 (1) ◽  
Author(s):  
Sungryong Oh ◽  
Joohee Jung
Keyword(s):  
Liver Cancer ◽  
Adverse Effects ◽  
Anticancer Drugs ◽  
Clinical Data ◽  
Xenograft Model ◽  
In Vitro Test ◽  
Incidence And Mortality ◽  
Significant Difference ◽  
Xenograft Models

Abstract Background The incidence and mortality of liver cancer show a great difference between the sexes. We established sex-dependent liver cancer xenograft models and investigated whether such sex-dependent models could be used to simultaneously evaluate the therapeutic and adverse effects of anticancer drugs for drug screening. Results In the in-vitro test, the cytotoxicity of anticancer drugs (cisplatin, 5-fluorouracil, and doxorubicin) was compared between male- and female-derived liver cancer cell lines. Cisplatin and 5-fluorouracil exhibited cytotoxicity without sex-difference, but doxorubicin showed dose-dependently significant cytotoxicity only in male-derived cells. Our results showed a strong correlation between preclinical and clinical data with the use of sex-dependent liver cancer xenograft models. Moreover, the male-derived Hep3B-derived xenograft model was more sensitive than the female-derived SNU-387-derived xenograft model against doxorubicin treatment. Doxorubicin showed more severe cardiotoxicity in the male xenograft model than in the female model. We investigated the occurrence frequency of doxorubicin-related cardiotoxicity using data obtained from the Korea Institute of Drug Safety & Risk Management Database, but no significant difference was observed between the sexes. Conclusions Our results suggest that sex-dependent xenograft models are useful tools for evaluating the therapeutic and adverse effects of anticancer drugs, because sex is an important consideration in drug development.

scholarly journals Effect of Gold Nanoparticles and Silicon on the Bioactivity and Antibacterial Properties of Hydroxyapatite/Chitosan/Tricalcium Phosphate-Based Biomicroconcretes

Materials ◽  
2021 ◽  
Vol 14 (14) ◽  
pp. 3854
Author(s):  
Joanna Czechowska ◽  
Ewelina Cichoń ◽  
Anna Belcarz ◽  
Anna Ślósarczyk ◽  
Aneta Zima
Keyword(s):  
Gold Nanoparticles ◽  
Antibacterial Activity ◽  
Tricalcium Phosphate ◽  
Setting Time ◽  
Antibacterial Properties ◽  
Bone Substitutes ◽  
Bacterial Strains ◽  
Setting Times ◽  
Biological Studies

Bioactive, chemically bonded bone substitutes with antibacterial properties are highly recommended for medical applications. In this study, biomicroconcretes, composed of silicon modified (Si-αTCP) or non-modified α-tricalcium phosphate (αTCP), as well as hybrid hydroxyapatite/chitosan granules non-modified and modified with gold nanoparticles (AuNPs), were designed. The developed biomicroconcretes were supposed to combine the dual functions of antibacterial activity and bone defect repair. The chemical and phase composition, microstructure, setting times, mechanical strength, and in vitro bioactive potential of the composites were examined. Furthermore, on the basis of the American Association of Textile Chemists and Colorists test (AATCC 100), adapted for chemically bonded materials, the antibacterial activity of the biomicroconcretes against S. epidermidis, E. coli, and S. aureus was evaluated. All biomicroconcretes were surgically handy and revealed good adhesion between the hybrid granules and calcium phosphate-based matrix. Furthermore, they possessed acceptable setting times and mechanical properties. It has been stated that materials containing AuNPs set faster and possess a slightly higher compressive strength (3.4 ± 0.7 MPa). The modification of αTCP with silicon led to a favorable decrease of the final setting time to 10 min. Furthermore, it has been shown that materials modified with AuNPs and silicon possessed an enhanced bioactivity. The antibacterial properties of all of the developed biomicroconcretes against the tested bacterial strains due to the presence of both chitosan and Au were confirmed. The material modified simultaneously with AuNPs and silicon seems to be the most promising candidate for further biological studies.

scholarly journals Synthesis, Characterization, Antibacterial Properties, and In Vitro Studies of Selenium and Strontium Co-Substituted Hydroxyapatite

2021 ◽  
Vol 22 (8) ◽  
pp. 4246
Author(s):  
Muhammad Maqbool ◽  
Qaisar Nawaz ◽  
Muhammad Atiq Ur Atiq Ur Rehman ◽  
Mark Cresswell ◽  
Phil Jackson ◽  
...  
Keyword(s):  
Negative Impact ◽  
Antibacterial Properties ◽  
Biological Properties ◽  
Ion Concentration ◽  
Charge Composition ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
Molar Ratios ◽  
Human Osteosarcoma Cells

In this study, as a measure to enhance the antimicrobial activity of biomaterials, the selenium ions have been substituted into hydroxyapatite (HA) at different concentration levels. To balance the potential cytotoxic effects of selenite ions (SeO32−) in HA, strontium (Sr2+) was co-substituted at the same concentration. Selenium and strontium-substituted hydroxyapatites (Se-Sr-HA) at equal molar ratios of x Se/(Se + P) and x Sr/(Sr + Ca) at (x = 0, 0.01, 0.03, 0.05, 0.1, and 0.2) were synthesized via the wet precipitation route and sintered at 900 °C. The effect of the two-ion concentration on morphology, surface charge, composition, antibacterial ability, and cell viability were studied. X-ray diffraction verified the phase purity and confirmed the substitution of selenium and strontium ions. Acellular in vitro bioactivity tests revealed that Se-Sr-HA was highly bioactive compared to pure HA. Se-Sr-HA samples showed excellent antibacterial activity against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus carnosus) bacterial strains. In vitro cell–material interaction, using human osteosarcoma cells MG-63 studied by WST-8 assay, showed that Se-HA has a cytotoxic effect; however, the co-substitution of strontium in Se-HA offsets the negative impact of selenium and enhanced the biological properties of HA. Hence, the prepared samples are a suitable choice for antibacterial coatings and bone filler applications.

scholarly journals Antibacterial Activity of Leukocyte- and Platelet-Rich Plasma: AnIn VitroStudy

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Agata Cieślik-Bielecka ◽  
Tadeusz Bold ◽  
Grzegorz Ziółkowski ◽  
Marcin Pierchała ◽  
Aleksandra Królikowska ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enterococcus Faecalis ◽  
Platelet Rich Plasma ◽  
Antibacterial Properties ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Bovine Thrombin ◽  
Zones Of Inhibition

The aim of the study was to investigate the leukocyte- and platelet-rich plasma (L-PRP) antimicrobial activity. The studied sample comprised 20 healthy males. The L-PRP gel, liquid L-PRP, and thrombin samples were testedin vitrofor their antibacterial properties against selected bacterial strains using the Kirby-Bauer disc diffusion method. Two types of thrombin were used (autologous and bovine). Zones of inhibition produced by L-PRP ranged between 6 and 18 mm in diameter. L-PRP inhibited the growth ofStaphylococcus aureus(MRSA and MSSA strains) and was also active againstEnterococcus faecalisandPseudomonas aeruginosa. There was no activity againstEscherichia coliandKlebsiella pneumoniae. The statistically significant increase of L-PRP antimicrobial effect was noted with the use of major volume of thrombin as an activator. Additionally, in groups where a bovine thrombin mixture was added to L-PRP the zones of inhibition concerning MRSA,Enterococcus faecalis, andPseudomonas aeruginosawere larger than in the groups with autologous thrombin. Based on the conducted studies, it can be determined that L-PRP can evokein vitroantimicrobial effects and might be used to treat selected infections in the clinical field. The major volume of thrombin as an activator increases the strength of the L-PRP antimicrobial effect.

In vitro Resistance Testing of Porphyromonas gingivalis, Prevotella intermedia, and Tannerella forsythia to Triclosan

2016 ◽  
Vol 17 (4) ◽  
pp. 282-285 ◽  
Author(s):  
Deema J Farsi ◽  
Anne Tanner
Keyword(s):  
Escherichia Coli ◽  
Porphyromonas Gingivalis ◽  
Vitamin K ◽  
Prolonged Exposure ◽  
Resistance Testing ◽  
Prevotella Intermedia ◽  
Periodontal Pathogens ◽  
Tannerella Forsythia ◽  
Prolonged Incubation

ABSTRACT Aim To determine the sensitivity of Porphyromonas gingivalis, Prevotella intermedia, and Tannerella forsythia to triclosan, and determine if these bacteria develop resistance to triclosan upon prolonged exposure. Materials and methods Susceptibility to triclosan was tested against three periodontal pathogens P. gingivalis, P. intermedia, and T. forsythia. Escherichia coli strains sensitive and resistant to triclosan were used as biological controls to confirm the efficacy of triclosan in the assays. Agar plates were prepared locally with vitamin K and hemin-supplemented medium. Results Porphyromonas gingivalis and P. intermedia did not grow on plates containing ≥2 μg/ml triclosan, while T. forsythia did not grow on ≥1.66 μg/ml. Colonies of P. intermedia resistant to triclosan developed after prolonged incubation at 2 μg/ml, but this resistance disappeared during subculture in the absence of triclosan. Conclusion No significant resistance to triclosan was detected for these species. Clinical significance Dental products containing triclosan can be beneficial in controlling periodontal disease. How to cite this article Farsi D, Tanner A. In vitro Resistance Testing of Porphyromonas gingivalis, Prevotella intermedia, and Tannerella forsythia to Triclosan. J Contemp Dent Pract 2016;17(4):282-285.

scholarly journals Inhibitory Effects of Lactoferrin on Growth and Biofilm Formation of Porphyromonas gingivalis and Prevotella intermedia

2009 ◽  
Vol 53 (8) ◽  
pp. 3308-3316 ◽  
Author(s):  
Hiroyuki Wakabayashi ◽  
Koji Yamauchi ◽  
Tetsuo Kobayashi ◽  
Tomoko Yaeshima ◽  
Keiji Iwatsuki ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Biofilm Formation ◽  
Periodontal Diseases ◽  
Antimicrobial Protein ◽  
Antibiofilm Activity ◽  
Prevotella Intermedia ◽  
Periodontopathic Bacteria ◽  
Oral Pathogens ◽  
In Vitro Effects

ABSTRACT Lactoferrin (LF) is an iron-binding antimicrobial protein present in saliva and gingival crevicular fluids, and it is possibly associated with host defense against oral pathogens, including periodontopathic bacteria. In the present study, we evaluated the in vitro effects of LF-related agents on the growth and biofilm formation of two periodontopathic bacteria, Porphyromonas gingivalis and Prevotella intermedia, which reside as biofilms in the subgingival plaque. The planktonic growth of P. gingivalis and P. intermedia was suppressed for up to 5 h by incubation with ≥130 μg/ml of human LF (hLF), iron-free and iron-saturated bovine LF (apo-bLF and holo-bLF, respectively), and ≥6 μg/ml of bLF-derived antimicrobial peptide lactoferricin B (LFcin B); but those effects were weak after 8 h. The biofilm formation of P. gingivalis and P. intermedia over 24 h was effectively inhibited by lower concentrations (≥8 μg/ml) of various iron-bound forms (the apo, native, and holo forms) of bLF and hLF but not LFcin B. A preformed biofilm of P. gingivalis and P. intermedia was also reduced by incubation with various iron-bound bLFs, hLF, and LFcin B for 5 h. In an examination of the effectiveness of native bLF when it was used in combination with four antibiotics, it was found that treatment with ciprofloxacin, clarithromycin, and minocycline in combination with native bLF for 24 h reduced the amount of a preformed biofilm of P. gingivalis compared with the level of reduction achieved with each agent alone. These results demonstrate the antibiofilm activity of LF with lower iron dependency against P. gingivalis and P. intermedia and the potential usefulness of LF for the prevention and treatment of periodontal diseases and as adjunct therapy for periodontal diseases.

The effect of condensed tannins fromLotus pedunculatusandLotus corniculatuson the growth of proteolytic rumen bacteria in vitro and their possible mode of action

2001 ◽  
Vol 47 (7) ◽  
pp. 626-633 ◽  
Author(s):  
A L Molan ◽  
G T Attwood ◽  
B R Min ◽  
W C McNabb
Keyword(s):  
Mode Of Action ◽  
Condensed Tannins ◽  
Large Subunit ◽  
Rumen Bacteria ◽  
Bacterial Cells ◽  
Bacterial Strains ◽  
Bisphosphate Carboxylase ◽  
Butyrivibrio Fibrisolvens ◽  
Significant Difference

Five strains of proteolytic rumen bacteria were treated with condensed tannins (CT) purified from Lotus pedunculatus and Lotus corniculatus to investigate their effect on the growth of these bacteria in vitro. Streptococcus bovis NCFB 2476, Eubacterium sp. C124b, Prevotella bryantii B14, Butyrivibrio fibrisolvens H17c, and Clostridium proteoclasticum B316Twere tested against 200, 400, and 600 µg CT·mL–1extracted from L. pedunculatus and L. corniculatus. In the absence of CT, all bacterial strains showed typical growth and reached maximum optical density (OD) after 6–8 h of incubation in a plant protein medium. Growth of Eubacterium sp., P. bryantii, and B. fibrisolvens was inhibited (P < 0.01–0.001) more by the CT from L. pedunculatus than by the CT from L. corniculatus. All strains continued to grow in the presence of 200 µg·mL–1of the CT from L. pedunculatus, but attained significantly (P < 0.05–0.01) lower maximum OD600values than (minus CT) controls, except for S. bovis. At 400 and 600 µg·mL–1, the addition of CT from L. pedunculatus inhibited (P < 0.05–0.001) the growth of all bacterial strains tested compared with controls. The growth of Eubacterium sp. and P. bryantii was stimulated for the first 4–6 h of incubation (P < 0.001) by 200 µg·mL–1of CT from L. corniculatus, but then declined leading to a significant difference in OD values compared with the controls. At 400 µg·mL–1, the CT from L. corniculatus reduced (P < 0.05–0.01) the growth of all strains except S. bovis, while 600 µg·mL–1inhibited (P < 0.01–0.001) the growth of all strains. To study the mechanism of CT action, the degradation of the large subunit (LSU) of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco; Fraction 1 Leaf protein) was followed after bacterial cells or Rubisco were preincubated with CT extracted from L. corniculatus and L. pedunculatus. Both preincubations decreased LSU degradation, but they differed in their response to polyethylene glycol (PEG) addition. Addition of PEG to CT–Rubisco preincubations negated the effects of CT, while PEG addition to CT–bacteria preincubations did not. This implies that the CT–bacterial interaction is stronger than the CT–Rubisco interaction or the interaction is of a different type. Also, L. pedunculatus CT reduced the degradation of the LSU to a greater extent than the CT from L. corniculatus when preincubated with bacteria.Key words: condensed tannins, growth, in vitro, proteolytic rumen bacteria, mode of action, Rubisco.

scholarly journals In vitro Evaluationof the Antimi- crobial Activity of a Topical Skin Preparation Containing 0.1% Polyhexanide vs a Topical Skin Prepa-ration Containing 1% Silver Sulfadiazine

2021 ◽  
Vol 6 (2) ◽  
pp. 1-7
Author(s):  
Barbara Maglione ◽  
Keyword(s):  
Antibacterial Activity ◽  
Positive Control ◽  
Negative Control ◽  
Silver Sulfadiazine ◽  
Bacterial Strains ◽  
Skin Preparation ◽  
E Coli ◽  
Topical Cream ◽  
Significant Difference

Aim: The effective in vitro antibacterial activity on Staphylococcus aureus (S.aureus), Pseudomonas aeruginosa (P.aeruginosa), Klebsiella pneumoniae (K.pneumoniae),Escherichia coli (E.Coli) and the combination of S.aureus and K. pneumonia of a topical cream based on 0.1% polyhexanidewas compared to a topical cream based on 1% silver sulfadiazine.A topical cream containing 0,1% gentamicin was used as a positive control and a white blank topical cream was used as negative control. Materials and Methods: The in vitro antibacterial activities were determined by agar well-diffusion assay. Two-way Analysis of Variance (ANOVA) was used to test, by calculation of P-values, for significant antiseptic activity in bacteria treated with 0.1% polyhexanide topical cream compared to 1% silver sulfadiazine and to the negative and positive controls. Results: Among the derivatives tested, all the active topical creams analyzed were able to reduce microbial strains. The topical cream based on 0.1% polyhexanide showed a significantly higher antibacterial efficacy in comparison to the topical cream based on 1% silver sulfadiazine on S. aureus and K. pneumonia and on the combination of S. aureus and K. pneumoniae,while no significant difference was detected between the antibacterial activity of the two topical creams against P. aeruginosa and E. coli. Conclusion: These results provide a further insight into the antibacterial activity of polyhexanide and its non-inferiority compared to silver sulfadiazine towards certain bacterial strains (P. aeruginosa and E. coli) and superiority towards other (S. aureus and K. pneumoniae)and support the use of 0.1% Polyhexanide topical preparation for the treatment of wounds that are infected or at risk of infection.

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