scholarly journals Milk From Women Diagnosed With COVID-19 Does Not Contain SARS-CoV-2 RNA but Has Persistent Levels of SARS-CoV-2-Specific IgA Antibodies

2021 ◽  
Vol 12 ◽  
Author(s):  
Ryan M. Pace ◽  
Janet E. Williams ◽  
Kirsi M. Järvinen ◽  
Courtney L. Meehan ◽  
Melanie A. Martin ◽  
...  
Keyword(s):  
Longitudinal Study ◽  
Passive Immunity ◽  
Receptor Binding Domain ◽  
Limited Data ◽  
Maternal Infection ◽  
Lactating Women ◽  
Milk Samples ◽  
Risks And Benefits ◽  
Iga Antibodies ◽  
Longitudinal Study Design

BackgroundLimited data are available regarding the balance of risks and benefits from human milk and/or breastfeeding during and following maternal infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).ObjectiveTo investigate whether SARS-CoV-2 can be detected in milk and on the breast after maternal coronavirus disease 2019 (COVID-19) diagnosis; and characterize concentrations of milk immunoglobulin (Ig) A specific to the SARS-CoV-2 spike glycoprotein receptor binding domain (RBD) during the 2 months after onset of symptoms or positive diagnostic test.MethodsUsing a longitudinal study design, we collected milk and breast skin swabs one to seven times from 64 lactating women with COVID-19 over a 2-month period, beginning as early as the week of diagnosis. Milk and breast swabs were analyzed for SARS-CoV-2 RNA, and milk was tested for anti-RBD IgA.ResultsSARS-CoV-2 was not detected in any milk sample or on 71% of breast swabs. Twenty-seven out of 29 (93%) breast swabs collected after breast washing tested negative for SARS-CoV-2. Detection of SARS-CoV-2 on the breast was associated with maternal coughing and other household COVID-19. Most (75%; 95% CI, 70-79%; n=316) milk samples contained anti-RBD IgA, and concentrations increased (P=.02) during the first two weeks following onset of COVID-19 symptoms or positive test. Milk-borne anti-RBD IgA persisted for at least two months in 77% of women.ConclusionMilk produced by women with COVID-19 does not contain SARS-CoV-2 and is likely a lasting source of passive immunity via anti-RBD IgA. These results support recommendations encouraging lactating women to continue breastfeeding during and after COVID-19 illness.

scholarly journals Rapid seroconversion and persistent functional IgG antibodies in severe COVID-19 patients correlates with an IL-12p70 and IL-33 signature

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ariel Munitz ◽  
L. Edry-Botzer ◽  
M. Itan ◽  
R. Tur-Kaspa ◽  
D. Dicker ◽  
...  
Keyword(s):  
Nucleocapsid Protein ◽  
Neutralizing Antibodies ◽  
Host Response ◽  
Humoral Response ◽  
Rapid Onset ◽  
Response Analysis ◽  
Receptor Binding Domain ◽  
Igg Antibodies ◽  
Viral Receptor ◽  
Iga Antibodies

AbstractDespite ongoing efforts to characterize the host response toward SARS-CoV-2, a major gap in our knowledge still exists regarding the magnitude and duration of the humoral response. Analysis of the antibody response in mild versus moderate/severe patients, using our new developed quantitative electrochemiluminescent assay for detecting IgM/IgA/IgG antibodies toward SARS-CoV-2 antigens, revealed a rapid onset of IgG/IgA antibodies, specifically in moderate/severe patients. IgM antibodies against the viral receptor binding domain, but not against nucleocapsid protein, were detected at early stages of the disease. Furthermore, we observed a marked reduction in IgM/IgA antibodies over-time. Adapting our assay for ACE2 binding-competition, demonstrated that the presence of potentially neutralizing antibodies is corelated with IgG/IgA. Finally, analysis of the cytokine profile in COVID-19 patients revealed unique correlation of an IL-12p70/IL33 and IgG seroconversion, which correlated with disease severity. In summary, our comprehensive analysis has major implications on the understanding and monitoring of SARS-CoV-2 infections.

scholarly journals Probiotic Supplementation and Human Milk Cytokine Profiles in Japanese Women: A Retrospective Study from an Open-Label Pilot Study

Nutrients ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 2285
Author(s):  
Tomoki Takahashi ◽  
Hirofumi Fukudome ◽  
Hiroshi M. Ueno ◽  
Shiomi Watanabe-Matsuhashi ◽  
Taku Nakano ◽  
...  
Keyword(s):  
Human Milk ◽  
Regression Models ◽  
Bifidobacterium Longum ◽  
Pilot Trial ◽  
Bacillus Coagulans ◽  
Open Label ◽  
Probiotic Supplementation ◽  
Lactating Women ◽  
Milk Samples ◽  
Cytokine Levels

The benefits of probiotic supplementation to lactating mothers on human milk cytokines are inconclusive. Thus, we performed a comprehensive open-label pilot trial analysis of 27 human milk cytokines in lactating women with allergies (one to three months postpartum) to determine the effect of supplementation with a mixture of new probiotic strains. Participants voluntarily joined the probiotic (n = 41) or no supplementation control (n = 19) groups. The probiotic group took three probiotic tablets (Lactobacillus casei LC5, Bifidobacterium longum BG7, and Bacillus coagulans SANK70258) daily for one to three months postpartum. Milk samples were collected at one, two, and three months postpartum, and cytokine levels were measured using multiplex assays. The effects were analyzed using multivariate regression models. Eleven cytokines showed a positive rate of over 50% in the milk samples throughout testing in both groups. The positive rates of IL-1 receptor antagonist and IL-7 changed significantly with lactation progression in logistic regression models after adjusting for time and supplementation, whereas rates of other cytokines showed no significant differences. The lactational change patterns of IL-10 concentrations differed significantly between the two groups. A short-term supplementation of probiotics affects human milk cytokine levels in lactating women with a possible placebo effect still existing. Future placebo-controlled studies are needed to support these results, based on the estimated sample sizes in this study.

Longitudinal Study of Methylmercury and Inorganic Mercury in Blood and Urine of Pregnant and Lactating Women, as Well as in Umbilical Cord Blood

2000 ◽  
Vol 84 (2) ◽  
pp. 186-194 ◽  
Author(s):  
Marie Vahter ◽  
Agneta Åkesson ◽  
Birger Lind ◽  
Ulla Björs ◽  
Andrejs Schütz ◽  
...  
Keyword(s):  
Longitudinal Study ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Inorganic Mercury ◽  
Lactating Women ◽  
Mercury In Blood

scholarly journals Investigating whether adverse prenatal and perinatal events are associated with non-clinical psychotic symptoms at age 12 years in the ALSPAC birth cohort

2009 ◽  
Vol 39 (9) ◽  
pp. 1457-1467 ◽  
Author(s):  
S. Zammit ◽  
D. Odd ◽  
J. Horwood ◽  
A. Thompson ◽  
K. Thomas ◽  
...  
Keyword(s):  
Longitudinal Study ◽  
Adverse Events ◽  
Birth Cohort ◽  
Early Development ◽  
Gestational Age ◽  
Apgar Score ◽  
Psychotic Symptoms ◽  
Maternal Infection ◽  
Increased Risk ◽  
Clinical Disorders

BackgroundNon-clinical psychosis-like symptoms (PLIKS) occur in about 15% of the population. It is not clear whether adverse events during early development alter the risk of developing PLIKS. We aimed to examine whether maternal infection, diabetes or pre-eclampsia during pregnancy, gestational age, perinatal cardiopulmonary resuscitation or 5-min Apgar score were associated with development of psychotic symptoms during early adolescence.MethodA longitudinal study of 6356 12-year-old adolescents who completed a semi-structured interview for psychotic symptoms in the Avon Longitudinal Study of Parents and Children (ALSPAC) birth cohort. Prenatal and perinatal data were obtained from obstetric records and maternal questionnaires completed during pregnancy.ResultsThe presence of definite psychotic symptoms was associated with maternal infection during pregnancy [adjusted odds ratio (OR) 1.44, 95% confidence interval (CI) 1.11–1.86, p=0.006], maternal diabetes (adjusted OR 3.43, 95% CI 1.14–10.36, p=0.029), need for resuscitation (adjusted OR 1.50, 95% CI 0.97–2.31, p=0.065) and 5-min Apgar score (adjusted OR per unit decrease 1.30, 95% CI 1.12–1.50, p<0.001). None of these associations were mediated by childhood IQ score. Most associations persisted, but were less strong, when including suspected symptoms as part of the outcome. There was no association between PLIKS and gestational age or pre-eclampsia.ConclusionsAdverse events during early development may lead to an increased risk of developing PLIKS. Although the status of PLIKS in relation to clinical disorders such as schizophrenia is not clear, the similarity between these results and findings reported for schizophrenia indicates that future studies of PLIKS may help us to understand how psychotic experiences and clinical disorders develop throughout the life-course.

Excretion of Iohexol and Metrizoate in Human Breast Milk

1987 ◽  
Vol 28 (5) ◽  
pp. 523-526 ◽  
Author(s):  
S. T. Nielsen ◽  
I. Matheson ◽  
J. N. Rasmussen ◽  
K. Skinnemoen ◽  
E. Andrew ◽  
...  
Keyword(s):  
Breast Milk ◽  
Low Dose ◽  
Decay Curve ◽  
Human Breast Milk ◽  
Human Breast ◽  
Blood Samples ◽  
Lactating Women ◽  
Milk Samples ◽  
Slow Decay ◽  
Contrast Medium Injection

Six lactating women undergoing contrast media examination had milk and blood taken to determine the rate and extent of excretion of iohexol (Omnipaque) (four mothers) and metrizoate (Isopaque) (two mothers). Blood samples were taken up to 45 minutes and milk samples up to 48 hours after the contrast medium injection. The excretion was low, reaching a maximum at 3 to 6 hours and showing a slow decay curve (t 1/2 = 15 to 108 hours). One mother, who was weaning her baby, showed a different excretion pattern. The amount excreted during 24 hours was about 0.5 per cent of the weight adjusted maternal dose for both iohexol and metrizoate. It is not likely, that such a low dose of poorly absorbed drug would cause any adverse effects in the infant, unless it is hypersensitive to the drug already. The authors consider breast feeding to be acceptable for mothers receiving iohexol or metrizoate.

scholarly journals Detection of cross-reactive IgA against SARS-CoV-2 spike 1 subunit in saliva

2021 ◽  
Author(s):  
Keiichi Tsukinoki ◽  
Tatsuo Yamamoto ◽  
Keisuke Handa ◽  
Mariko Iwamiya ◽  
Juri Saruta ◽  
...  
Keyword(s):  
Breast Milk ◽  
Recombinant Protein ◽  
University Hospital ◽  
Spike Protein ◽  
Secretory Iga ◽  
Enzyme Linked Immunosorbent Assay ◽  
Receptor Binding Domain ◽  
Blood Samples ◽  
Milk Samples ◽  
Mucosal Surfaces

AbstractAbundant secretory IgA (sIgA) in mucus, breast milk, and saliva provides immunity that prevents infection of mucosal surfaces. sIgA in pre-pandemic breast milk samples have been reported to cross-react with SARS-CoV-2, but whether it also occurs in saliva and, if so, whether it cross-reacts with SARS-CoV-2, has remained unknown. We aimed to clarify whether sIgA in saliva cross-reacts with SARS-CoV-2 spike 1 subunit in individuals who have not been infected with this virus. The study included 137 (male, n = 101; female, n = 36; mean age, 38.7 [24–65] years) of dentists and doctors in the Kanagawa Dental University Hospital. Saliva and blood samples were analyzed by PCR and immunochromatography for IgG and IgM, respectively. We then identified patients with saliva samples that were confirmed as PCR- and IgM-negative for COVID-19. Proportions of SARS-CoV-2 cross-reactive IgA-positive individuals were determined by enzyme-linked immunosorbent assay using a biotin-labeled spike S1-mFc recombinant protein covering the receptor-binding domain of SARS-CoV-2. The proportion of SARS-CoV-2 cross-reactive IgA-positive individuals was 46.7%, and this correlated negatively with age (r = −0.218, p = 0.01). The proportion of IgA-positive individuals ≥ 50 y was significantly lower than that of patients aged ≤ 49 y (p = 0.008). sIgA was purified from the saliva of all patients, and the salivary sIgA was found to suppress the binding of SARS-CoV-2 spike protein to the ACE-2 receptor. We found SARS-CoV-2 cross-reactive sIgA in the saliva of some participants who had never been infected with the virus, suggesting that sIgA helps prevent SARS-CoV-2 infection.

scholarly journals Risk taking, perceived risks, and perceived benefits across adolescence: A domain-specific risk-return approach

2021 ◽  
Author(s):  
Neeltje Blankenstein ◽  
Jorien van Hoorn ◽  
Tycho Dekkers ◽  
Arne Popma ◽  
Brenda Jansen ◽  
...  
Keyword(s):  
Longitudinal Study ◽  
Risk Taking ◽  
Control Group ◽  
Perceived Benefits ◽  
Perceived Risks ◽  
Domain Specific ◽  
Risk Return ◽  
Risks And Benefits ◽  
Specific Risk ◽  
Adolescent Risk

Adolescence is a phase of heightened risk taking compared to childhood and adulthood, which is even more prominent for specific adolescent populations, such as youth with attention-deficit/hyperactivity disorder (ADHD). Until now little is known about how perceived risks and benefits relate to adolescent risk taking. Here, we used the adolescent version of the Domain-Specific Risk-Taking (DoSpeRT) scale to investigate the likelihood of risk taking, perceived risks, perceived benefits, and their tradeoff in two studies. In the first longitudinal study, 375 11-to-23-year-olds completed the DOSPERT one up to three times. A second biannual longitudinal study included 180 11-to-20-year old boys diagnosed with ADHD (N=81), and an IQ and age-matched control group (N=99). Using mixed-effects models, we found a peak in likelihood of risk taking in mid-to-late adolescence, but only in the health/safety, ethical, and social domains of risk taking, with similar curvilinear patterns in perceived benefits (peaks) and perceived risks (dips). In both cohorts, perceived risks and benefits were significant predictors of risk taking in all domains, and perceived benefits related more strongly to risk taking than perceived risks. Moreover, perceived benefits increasingly related to risk taking across adolescence, a pattern that was found in recreational risk taking in both studies. Generally, we observed little differences in risk taking, and perceived risks and benefits between the ADHD and control group. However, risk-return models indicated that adolescents with ADHD displayed a heightened likelihood of risk-taking behavior in the social domain, and their perceived risks related less strongly to risk taking, relative to typically developing adolescents. Taken together, our results are consistent with the developmental peak in risk taking observed in real life and highlight the role of perceived risks and benefits in risk taking. These findings provide tentative entry points for possible prevention and intervention.

scholarly journals The pitch of babies’ cries predicts their voice pitch at age 5

2018 ◽  
Vol 14 (7) ◽  
pp. 20180065 ◽  
Author(s):  
Florence Levrero ◽  
Nicolas Mathevon ◽  
Katarzyna Pisanski ◽  
Erik Gustafsson ◽  
David Reby
Keyword(s):  
Longitudinal Study ◽  
Individual Differences ◽  
Fundamental Frequency ◽  
Ring Finger ◽  
Small Sample ◽  
Voice Pitch ◽  
Same Sex ◽  
Before And After ◽  
Longitudinal Study Design ◽  
The Right

Voice pitch (fundamental frequency, F 0 ) is a key dimension of our voice that varies between sexes after puberty, and also among individuals of the same sex both before and after puberty. While a recent longitudinal study indicates that inter-individual differences in voice pitch remain stable in men during adulthood and may even be determined before puberty (Fouquet et al. 2016 R. Soc. open sci. 3 , 160395. ( doi:10.1098/rsos.160395 )), whether these differences emerge in infancy remains unknown. Here, using a longitudinal study design, we investigate the hypothesis that inter-individual differences in F 0 are already present in the cries of pre-verbal babies. While based on a small sample ( n = 15), our results indicate that the F 0 of babies' cries at 4 months of age may predict the F 0 of their speech utterances at 5 years of age, explaining 41% of the inter-individual variance in voice pitch at that age in our sample. We also found that the right-hand ratio of the length of their index to ring finger (2D : 4D digit ratio), which has been proposed to constitute an index of prenatal testosterone exposure, was positively correlated with F 0 at both 4 months and 5 years of age. These findings suggest that a substantial proportion of between-individual differences in voice pitch, which convey important biosocial information about speakers, may partly originate in utero and thus already be present soon after birth.

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