scholarly journals Distribution and Diversity of Sand Fly Species (Diptera: Psychodidae, Phlebotominae) in Two Geoclimatic Zones of Chad

2022 ◽  
Vol 2 ◽  
Author(s):  
Demba Kodindo Israël ◽  
Cheick Amadou Coulibaly ◽  
Ibrahim Moussa Sissoko ◽  
Bourama Traoré ◽  
André B. B. Wilke ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Vector Competence ◽  
Abundant Species ◽  
Morphological Characters ◽  
Sand Fly ◽  
Canine Leishmaniasis ◽  
Phlebotomus Duboscqi ◽  
Vector Borne ◽  
Study Sites ◽  
Health Districts

Leishmaniasis transmitted by sandflies is an important vector-borne disease. In Chad, information on sandflies is outdated, and so this study was designed to update the sandfly fauna. Sandflies were collected in five health districts representing two geoclimatic zones using sticky traps and pyrethrum sprays in indoor and peridomestic habitats between September 2019 and December 2020. All collected sandfly specimens were identified based on species-level morphological characters. A total of 2,015 specimens belonging to 13 species of sandflies (one Phlebotomus and twelve Sergentomyia) were collected and identified. The vector of human cutaneous leishmaniasis, Phlebotomus duboscqi, represents 0.65% of all collected sandflies and is the only representative of the Phlebotomus genus to be collected predominantly inside human dwellings. Phlebotomus orientalis, the vector of visceral leishmaniasis previously collected in Chad in 1976 was not found in this study. Sergentomyia clydei, Sergentomyia schwetzi, Sergentomyia antennata and Sergentomyia africana were the most abundant species collected with 44.71%; 22.73%; 16.03% and 11.17%, respectively. Sergentomyia schwetzi and Sergentomyia dubia, the two species involved in the transmission of canine leishmaniasis, were found in five and four study sites respectively. According to our results, the sandfly fauna of the two geoclimatic zones of Chad is comprised of 13 species. Our data showed that, unlike P. orientalis which was not found, P. duboscqi is present in four of the five sites surveyed. Therefore, these areas are at risk and remain potential foci of cutaneous leishmaniasis. However, the need for further studies such as vector species detection, their seasonal fluctuations and their vector competence.

scholarly journals Distribution and Diversity of Sand Fly Species (Diptera: Psychodidae, Phlebotominae) in Two Geoclimatic Zones of Chad

2021 ◽  
Author(s):  
Israel DEMBA KODINDO ◽  
Demba Kodindo Israël ◽  
Cheick Amadou Coulibaly ◽  
Ibrahim Moussa Sissoko ◽  
Bourama Traoré ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Vector Competence ◽  
Abundant Species ◽  
Morphological Characters ◽  
Sand Fly ◽  
Canine Leishmaniasis ◽  
Phlebotomus Duboscqi ◽  
Vector Borne ◽  
Study Sites ◽  
Health Districts

Abstract Introduction: Leishmaniasis transmitted by sandflies is an important vector-borne disease. In Chad, information on sandflies is outdated, and so this study was designed to update the sandfly fauna. Methods: Sandflies were collected in five health districts representing two geoclimatic zones using sticky traps and pyrethrum sprays in indoor and peridomestic habitats between September 2019 and December 2020. All collected sandfly specimens were identified based on species-level morphological characters. Results: A total of 2,015 specimens belonging to 13 species of sandflies (one Phlebotomus and twelve Sergentomyia) were collected and identified. The vector of human cutaneous leishmaniasis, Phlebotomus duboscqi, represents 0.65% of all collected sandflies and is the only representative of the Phlebotomus genus to be collected predominantly inside human dwellings. Phlebotomus orientalis, the vector of visceral leishmaniasis previously collected in Chad in 1976 was not found in this study. Sergentomyia clydei, Sergentomyia schwetzi, Sergentomyia antennata and Sergentomyia africana were the most abundant species collected with 44.71%; 22.73%; 16.03% and 11.17%, respectively. Sergentomyia schwetzi and Sergentomyia dubia, the two species involved in the transmission of canine leishmaniasis, were found in five and four study sites respectively. Conclusion: According to our results, the sandfly fauna of the two geoclimatic zones of Chad is comprised of 13 species. Our data showed that, unlike P. orientalis which was not found, P. duboscqi is present in four of the five sites surveyed. Therefore, these areas are at risk and remain potential foci of cutaneous leishmaniasis. However, the need for further studies such as vector species detection, their seasonal fluctuations and their vector competence.

scholarly journals Morphological Characterization of the Antennal Sensilla of the Afrotropical Sand Fly, Phlebotomus duboscqi (Diptera: Psychodidae)

2020 ◽  
Author(s):  
Ana Cristina Bahia ◽  
Ana Beatriz F Barletta ◽  
Luciana Conceição Pinto ◽  
Alessandra S Orfanó ◽  
Rafael Nacif-Pimenta ◽  
...  
Keyword(s):  
Morphological Characters ◽  
Morphological Characterization ◽  
Sand Fly ◽  
Antennal Sensilla ◽  
Olfactory Sensilla ◽  
Zoonotic Cutaneous Leishmaniasis ◽  
Electrophysiological Studies ◽  
Phlebotomus Duboscqi ◽  
Well Differentiated ◽  
Function Number

Abstract We investigated by scanning electron microscopy the morphology, distribution, and abundance of antennal sensilla of females Phlebotomus duboscqi sand fly, an important vector of zoonotic cutaneous leishmaniasis at Afrotropical region. Thirteen well-differentiated sensilla were identified, among six types of cuticular sensilla. The probable function of these sensillary types is discussed in relation to their external structure and distribution. Five sensillary types were classified as olfactory sensilla, as they have specific morphological characters of sensilla with this function. Number and distribution of sensilla significantly differed between antennal segments. The results of the present work, besides corroborating in the expansion of the morphological and ultrastructural knowledge of P. duboscqi, can foment future electrophysiological studies for the development of volatile semiochemicals, to be used as attractants in traps for monitoring and selective vector control of this sand fly.

scholarly journals A sand fly salivary protein vaccine shows efficacy against vector-transmitted cutaneous leishmaniasis in nonhuman primates

2015 ◽  
Vol 7 (290) ◽  
pp. 290ra90-290ra90 ◽  
Author(s):  
Fabiano Oliveira ◽  
Edgar Rowton ◽  
Hamide Aslan ◽  
Regis Gomes ◽  
Philip A. Castrovinci ◽  
...  
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Nonhuman Primates ◽  
Mononuclear Cells ◽  
Rhesus Macaques ◽  
Parasite Burden ◽  
Salivary Protein ◽  
Sand Fly ◽  
Protein Vaccine ◽  
Peripheral Blood Mononuclear ◽  
Phlebotomus Duboscqi

Currently, there are no commercially available human vaccines against leishmaniasis. In rodents, cellular immunity to salivary proteins of sand fly vectors is associated to protection against leishmaniasis, making them worthy targets for further exploration as vaccines. We demonstrate that nonhuman primates (NHP) exposed to Phlebotomus duboscqi uninfected sand fly bites or immunized with salivary protein PdSP15 are protected against cutaneous leishmaniasis initiated by infected bites. Uninfected sand fly–exposed and 7 of 10 PdSP15-immunized rhesus macaques displayed a significant reduction in disease and parasite burden compared to controls. Protection correlated to the early appearance of Leishmania-specific CD4+IFN-γ+ lymphocytes, suggesting that immunity to saliva or PdSP15 augments the host immune response to the parasites while maintaining minimal pathology. Notably, the 30% unprotected PdSP15-immunized NHP developed neither immunity to PdSP15 nor an accelerated Leishmania-specific immunity. Sera and peripheral blood mononuclear cells from individuals naturally exposed to P. duboscqi bites recognized PdSP15, demonstrating its immunogenicity in humans. PdSP15 sequence and structure show no homology to mammalian proteins, further demonstrating its potential as a component of a vaccine for human leishmaniasis.

scholarly journals Cutaneous Leishmaniasis in Pakistan: a neglected disease needing one health strategy

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Behzad Kayani ◽  
Shakera Sadiq ◽  
Hamad Bin Rashid ◽  
Naseer Ahmed ◽  
Altaf Mahmood ◽  
...  
Keyword(s):  
Risk Factors ◽  
Cutaneous Leishmaniasis ◽  
One Health ◽  
Protective Factor ◽  
Sand Fly ◽  
Canine Leishmaniasis ◽  
Sanitary Condition ◽  
Health Strategy ◽  
Potential Risk Factors ◽  
A Prospective Study

Abstract Background Cutaneous Leishmaniasis (CL) is a neglected tropical disease, which mainly affects poor communities. It is one of the major vector-borne disease and endemic in Pakistan. Methods A case-control study to evaluate potential risk factors of human-CL was conducted in Khewra region, District Jhelum, Pakistan from January–April 2014. Case data about 90 cases registered during October 2012 to November 2013 was retrieved from Municipal Hospital. Controls were matched (1,1 ratio) on the date of registration with cases from same hospital. Both cases and controls were invited to participate and data was collected in a face-to-face interview. A prospective study of canine leishmaniasis (canine-CL) was also conducted at Civil Veterinary Hospital in the same area. Suspected dogs with skin ulceration signs were included in the study and blood samples were collected. Statistical analyses were conducted to determine association between various parameters and outcome of interest. Results The ages of cases ranged from 1 to 76 years (median = 15 years) and proved to be protective factor i.e. increase in each year in age reduced the likelihood of being infected with human-CL [Odds Ratio (OR) = 0.4, 95% Confidence Interval (CI) = 0.25–0.76]. People sleeping outsides in an open area were more likely to become a case (OR = 8.7, 95% CI = 2.90–26.37) than a control. Poor sanitary condition inside the house (OR = 3.3, 95% CI 1.03–10.56) and presence of other animals in house (livestock, poultry) (OR = 3.6, 95% CI = 1.07–12.12) also identified as risk factors of high significance. The proportion of positive dogs with canine-CL was 21.05% and was significantly associated with human-CL cases in the same area (p < 0.05). Conclusions We concluded that adopting self-protections measures against sand-fly, and maintaining good hygiene may lower the risk of human-CL. One-Health Strategy is suggested to control leishmaniasis in human and dog population.

scholarly journals The sand fly (Diptera: Psychodidae) fauna of the urban area of Lassance, Northeast Minas Gerais, Brazil

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0257043
Author(s):  
Gabriel Barbosa Tonelli ◽  
Camila Binder ◽  
Victoria Laporte Carneiro Nogueira ◽  
Marina Henriques Prado ◽  
Gabriela Gonçalves Theobaldo ◽  
...  
Keyword(s):  
Leishmania Infantum ◽  
Abundant Species ◽  
Minas Gerais ◽  
Sand Fly ◽  
Canine Leishmaniasis ◽  
Lutzomyia Longipalpis ◽  
Scaling Analysis ◽  
Sand Flies ◽  
Risk Area ◽  
Risk Areas

The present study aimed to check the sand flies’ fauna on the municipality of Lassance, Minas Gerais, Brazil and detect the presence of Leishmania DNA on the female captured and determine the risk areas of the municipality. Sand flies were collected monthly from May 2018 to April 2019 using automatic light traps for 3 consecutive nights. Eight houses were selected as sample points due its previous reports of tegumentary leishmaniasis and/or canine leishmaniasis. The sand fly’s fauna found on the present study it’s represented by several medical importance species and the most abundant species found were Lutzomyia longipalpis (77.09%) and Nyssomyia intermedia (10.06%). Leishmania infantum DNA was detected in a pool of Lu. longipalpis resulting on a 2.81% of infection rate. By the frequency of the two most abundant species on this study, we developed a risk area map and it draws attention to sample point 6 due to disparate abundance of sand flies at this site (81.81%). Statistical overview shows Lu. longipalpis as dominant species and, still, Non-Metric Multidimensional Scaling analysis reveal high similarity on fauna’s diversity on the study area. Our findings suggest that the diversity of sand flies from the municipality of Lassance may promote the circulation of Leishmania infantum parasites putting in risk the habitants and other mammal’s species. Still, our study reinforces the necessity of specific studies focused on breed sites of phlebotomine and its’ ecology to expand the knowledge about the behaviour of this group of insects applying directly to leishmaniases’ epidemiology.

scholarly journals Combating Leishmaniasis through Awareness Campaigning: A Mathematical Study on Media Efficiency

2016 ◽  
Vol 1 (3) ◽  
pp. 139-149 ◽  
Author(s):  
Dibyendu Biswas ◽  
Abhirup Datta ◽  
Priti Kumar Roy
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Disease Prevalence ◽  
Sand Fly ◽  
Mathematical Study ◽  
Complex Vector ◽  
Awareness Campaign ◽  
Infected Female ◽  
Vector Borne ◽  
Awareness Programme ◽  
Genus Leishmania

Leishmaniasis is a complex vector-borne disease that is originated by protozoa of the genus Leishmania. The disease is transmitted to human or animals through bites of infected female Phlebotominae sand-fly. Awareness campaign plays a significant role to restrict the spreading of disease cutaneous leishmaniasis. Here, we have considered a mathematical model of cutaneous leishmaniasis (CL) consisting susceptible and infected population of human and vector. Increase in growth of aware population impulsively leading to reduction of incompetence for environmental factors. Our analytical and numerical results reveal that circulating of the campaigning periodically minimizes the disease prevalence. The efficiency of the awareness programme contributes a better regime on the system moving towards healthy and hygienic environment.

Ecology of Sand Flies (Psychodidae: Phlebotominae) and Natural Infection of Pintomyia townsendi With Leishmania amazonensis in a Cutaneous Leishmaniasis Focus in Colombia

2020 ◽  
Vol 57 (5) ◽  
pp. 1653-1658
Author(s):  
Juliana Hoyos ◽  
Ranulfo González ◽  
Maria E Cuellar ◽  
Cielo León
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Natural Infection ◽  
Weather Conditions ◽  
Abundant Species ◽  
Epidemiological Studies ◽  
Leishmania Amazonensis ◽  
Sand Fly ◽  
Sand Flies ◽  
First Time ◽  
Positive Effect

Abstract Humans have influenced the epidemiological patterns of American cutaneous leishmaniasis by habitat disturbance, which has led to the emergence of new transmission foci. In these transmission areas, detecting natural infection of sand fly species with Leishmania parasites is of prime importance in epidemiological studies. In this study, we examined the species composition, spatial distribution, seasonality, and natural infection with Leishmania of the sand fly fauna in an emergent leishmaniasis focus located in Colombia. Sand flies were collected from September 2014 to June 2015 using CDC light traps located in indoor, peridomestic, and outdoor habitats within areas with confirmed clinical patients. In total, 13,488 sand flies of 12 species and seven genera were collected. Among these, Pintomyia townsendi (Ortiz) was the most abundant species comprising 76.3% of total flies collected. The sand fly richness and abundance were influenced by habitat and weather conditions. Outdoor areas harbored the greatest diversity of sand flies. Rainfall negatively affected abundance, whereas increased temperature showed a low positive effect. We detected Leishmania amazonensis parasites in Pi. townsendi for the first time.

scholarly journals Studies on the feeding habits of Lutzomyia (N.) intermedia (Diptera, Psychodidae), vector of cutaneous leishmaniasis in Brazil

2005 ◽  
Vol 21 (6) ◽  
pp. 1816-1820 ◽  
Author(s):  
Margarete Martins dos Santos Afonso ◽  
Almério Castro Gomes ◽  
Cláudio Roberto Valente Meneses ◽  
Elizabeth Ferreira Rangel
Keyword(s):  
Cutaneous Leishmaniasis ◽  
Blood Meal ◽  
Feeding Habits ◽  
Vector Competence ◽  
Domestic Animals ◽  
Sand Fly ◽  
Specific Antisera ◽  
Transmission Area ◽  
Precipitin Test ◽  
Janeiro State

The precipitin test was applied to identify the blood meal sources of Lutzomyia intermedia, collected in two distinct habitats: inside houses and in the peridomicile, in Mesquita, Rio de Janeiro State, Brazil, a transmission area of Leishmania (V.) braziliensis. The following antisera were tested: human, avian, equine, rodent, and opossum. From a total of 370 females analyzed, 128 specimens from the domicile and 59 from the peridomicile reacted with specific antisera. The anthropophily of L. intermedia was confirmed in both habitats; likewise, the feeding of this sand fly species on domestic animals, observed in previous entomological surveys, was confirmed by the strong reactivity with avian, canine, and equine antisera. However, feeding on rodents, mammals frequently found inside and around houses, represents further evidence related to the vector competence of L. intermedia, since synanthropic and sylvatic rodents have been considered a putative reservoir of L. (V.) braziliensis.

scholarly journals Clinical and laboratory profiles of visceral leishmaniasis among adult patients admitted to Felege Hiwot Hospital, Bahir Dar, Ethiopia

2021 ◽  
Vol 9 ◽  
pp. 205031212110367
Author(s):  
Berhanu Tarekegn ◽  
Ayanaw Tamene
Keyword(s):  
Visceral Leishmaniasis ◽  
Leishmania Donovani ◽  
Clinical Presentation ◽  
Winter Season ◽  
Adult Patients ◽  
Sand Fly ◽  
Timely Initiation ◽  
Vector Borne Disease ◽  
Vector Borne ◽  
Endemic Areas

Background: Visceral leishmaniasis is a vector-borne disease caused by Leishmania donovani transmitted by sand fly species. It is the third most common vector-borne disease globally. Visceral leishmaniasis is endemic in Ethiopia with an estimated annual incidence ranging from 3700 to 7400 cases. This research aimed to assess the clinical presentations and laboratory profiles of visceral leishmaniasis for early diagnosis and timely initiation of management. Objective: To describe the clinical and laboratory manifestation and diagnostic modalities of visceral leishmaniasis among adult patients admitted to Felege Hiwot Hospital, from 1 September 2016 to 30 August 2019. Method: Institution-based retrospective cross-sectional study was conducted among 141 patients admitted to Felege Hiwot Hospital from 1 September 2016 to 30 August 2019. Descriptive statistics were used to describe the clinical presentation and laboratory profiles of patients with visceral leishmaniasis. Results: Among a total of 141 enrolled patients in the study, males were affected 13-fold. Most of them were travelers to endemic areas during the winter season for labor work. The mean duration of illness was 48 days. Common symptoms were fever (96.5%), weightless (82.5%), jaundice (18.4%), vomiting/diarrhea (13.5%), and bleeding episodes (11.3%). Splenomegaly was seen in 98.6%, ascites in 35.5%, and lymphadenopathy in 9.9%. Lymphadenopathy was seen significantly in HIV patients (40%). Anemia was seen in 95%, thrombocytopenia in 90.2%, leukopenia in 86.4%, and pancytopenia in 79.4%. Half of the patients had coinfection. Neutropenic sepsis was seen in 21.3%. The diagnosis was made by tissue aspiration in 65% of patients. Conclusion: The majority of patients who were diagnosed to have visceral leishmaniasis were young male adults who traveled to the endemic areas seasonally. Fever and splenomegaly were seen as the commonest clinical presentation. Lymphadenopathy occurred in high frequency among HIV co-infected patients. Anemia was the commonest hematologic finding.

scholarly journals Phyllostomidae assemblage (Chiroptera: Mammalia) in altitudinal forests at the Parque Estadual do Ibitipoca, Southeast of Minas Gerais, Brazil

2016 ◽  
Vol 106 (0) ◽  
Author(s):  
Rodrigo M. Mello ◽  
Pedro H. Nobre ◽  
Marco A. Manhães ◽  
Alexmar S. Rodrigues
Keyword(s):  
Species Composition ◽  
Floristic Composition ◽  
Abundant Species ◽  
Minas Gerais ◽  
Forest Types ◽  
Mountainous Areas ◽  
Ecological Relevance ◽  
Forest Region ◽  
Study Sites ◽  
Artibeus Lituratus

ABSTRACT Many studies have demonstrated the ecological relevance and great biodiversity of bats in Brazil. However, mountainous areas have been disproportionately less sampled, mainly in the Southeast. The aim of this study was to identify and compare the richness and diversity of Phyllostomidae, the most diverse bat family, in different forest types in Parque Estadual do Ibitipoca, trying to understand the causes of possible differences. The Parque Estadual do Ibitipoca is inserted in the Serra da Mantiqueira's domain, in an Atlantic Forest region known as "Zona da Mata", state of Minas Gerais, with an altitudinal range between 1200-1784 meters. The study was conducted in two forest types, classified as "Nanofloresta Nebular" and "Floresta Nebular", whose respective data on richness and diversity were compared. The bats were captured with 8-10 mist nets for 14 months (April 2011 to May 2012) and four nights per month totaling 62,171.25 m2h of capture effort. A total of 392 captures (12 species) belonging to the Phyllostomidae family were obtained. The most abundant species were Sturnira lilium (59.9%), Platyrrhinus lineatus (11.3%), Artibeus lituratus (8.7%) and Carollia perspicillata (7.6%). The two sampled areas presented differences in bat richness, diversity and species composition, and this difference was predominantly influenced by S. lilium. It is likely that the observed difference in the assembly of bats between the two study sites depends on the variation in floristic composition. The records of A. lituratus and P. lineatus in a few months of the year and close to Ficus mexiae bearing ripe fruits suggests that at least these species move to the park for a few periods of the year in search of food resources, possibly moving through the altitudinal landscapes.

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