Effects of Ocean Acidification, Hypoxia, and Warming on the Gut Microbiota of the Thick Shell Mussel Mytilus coruscus Through 16S rRNA Gene Sequencing

Gut microbiota play a very important role in the health of the host, such as protecting from pathogens and maintaining homeostasis. However, environmental stressors, such as ocean acidification, hypoxia, and warming can affect microbial communities by causing alteration in their structure and relative abundance and by destroying their network. The study aimed to evaluate the combined effects of low pH, low dissolved oxygen (DO) levels, and warming on gut microbiota of the mussel Mytilus coruscus. Mussels were exposed to two pH levels (8.1, 7.7), two DO levels (6, 2 mg L−1), and two temperature levels (20, 30°C) for a total of eight treatments for 30 days. The experiment results showed that ocean acidification, hypoxia, and warming affected the community structure, species richness, and diversity of gut microbiota. The most abundant phyla noted were Proteobacteria, Bacteroidetes, and Firmicutes. Principal coordinate analysis (PCoA) revealed that ocean acidification, hypoxia, and warming change microbial community structure. Low pH, low DO, and increased temperature can cause shifting of microbial communities toward pathogen dominated microbial communities. Linear discriminant analysis effect size (LEfSe) showed that the significantly enriched biomarkers in each group are significantly different at the genus level. Phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) analysis revealed that the gut microbiome of the mussels is associated with many important functions, such as amino acid transport and metabolism, transcription, energy production and conservation, cell wall, membrane and envelope biogenesis, and other functions. This study highlights the complexity of interaction among pH, DO, and temperature in marine organisms and their effects on the gut microbiota and health of marine mussels.

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The Devils Hole pupfish gut microbiome and its potential role in paradoxical anaerobism

10.21203/rs.2.19358/v1 ◽

2019 ◽

Author(s):

Shrikant S Bhute ◽

Brisa Escobedo ◽

Mina Haider ◽

Yididya Mekonen ◽

Dafhner Ferrer ◽

...

Keyword(s):

Gut Microbiota ◽

Microbial Communities ◽

Critical Role ◽

16S Rrna Gene Sequencing ◽

Wide Distribution ◽

Control Group ◽

Rrna Gene ◽

Dependent Manner ◽

Bacterial Phyla ◽

Devils Hole

Abstract Background The Devils Hole Pupfish ( Cyprinodon diabolis ) frequently enters paradoxical anaerobism in response to endogenously produced or exogenously supplied ethanol in a dose-dependent manner. To decipher the role of the gut microbiota in ethanol-associated paradoxical anaerobism, gut microbial communities were depleted using a cocktail of antibiotics and profiled using 16S rRNA gene sequencing. Results Compared to the control group (n=12), microbiota-depleted fish (n=11) spent more time in paradoxical anaerobism. Our analysis indicated that the bacterial phyla Proteobacteria , Fusobacteria , Bacteroidetes , Firmicutes , Actinobacteria , Patescibacteria , and Dependentiae dominated the pupfish gut, which is consistent with other fish gut microbiota. Although the gut microbial communities with and without antibiotic treatment were similarly diverse, they were distinct and the greatest contribution to the dissimilarity (27.38%) was the common fish commensal Cetobacterium . We speculate that Cetobacterium , a primary fermenter, also consumes ethanol through secondary fermentation via an alcohol dehydrogenase and therefore regulates the transition from paradoxical anaerobism to aerobic respiration in fish. Conclusions This study reports the first characterization of gut microbial communities of critically endangered pupfish and suggests the microbiome may play a critical role in regulating metabolic strategies that are critical for survival in extremes of temperature and oxygen concentration. Given the wide distribution and abundance of Cetobacterium in warm-water fishes, this process may be of broad importance, and suggests that the microbiome be carefully considered for both conservation and aquaculture.

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Differences in the gut microbiomes of distinct ethnicities within the same geographic area are linked to host metabolic health

10.1101/2020.10.23.352807 ◽

2020 ◽

Author(s):

Qi Yan Ang ◽

Diana L. Alba ◽

Vaibhav Upadhyay ◽

Jordan E. Bisanz ◽

Jingwei Cai ◽

...

Keyword(s):

Community Structure ◽

Gut Microbiota ◽

Dietary Intake ◽

Ethnic Groups ◽

San Francisco ◽

Geographical Location ◽

16S Rrna Gene Sequencing ◽

Geographic Area ◽

Rrna Gene ◽

Starting Point

ABSTRACTBackgroundThe human gut microbiota exhibits marked variation around the world, which has been attributed to dietary intake and other environmental factors. However, the degree to which ethnicity-associated differences in gut microbial community structure and function are maintained following immigration or in the context of metabolic disease is poorly understood.ResultsWe conducted a multi-omic study of 46 lean and obese East Asian (EA) and White (W) participants living in the San Francisco Bay Area. 16S rRNA gene sequencing revealed significant differences between ethnic groups in bacterial richness and community structure. W individuals were enriched for the mucin-degrading Akkermansia muciniphila. EA participants had increased levels of multiple Bacteroidetes species, fermentative pathways detected by metagenomics, and the short-chain fatty acid end products acetate, propionate, and isobutyrate. Differences in the gut microbiota between the EA and W groups could not be explained by reported dietary intake, were more pronounced for lean individuals, and were associated with current geographical location. Microbiome transplantations into germ-free mice confirmed that the differences in the gut microbiota of the EA and W individuals we analyzed are indeed independent of diet and that they differentially impact host body weight and adiposity in genetically identical mouse recipients.ConclusionsThe reported findings emphasize the utility of studying diverse ethnic groups within a defined geographical location and provide a starting point for dissecting the mechanisms contributing to the complex interactions between the gut microbiome and ethnicity-associated lifestyle, demographic, metabolic, and genetic factors.

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Effects of Chronic Exposure to Microcystin-LR on the Gut Microbiota of Male Mice

International Journal of Toxicology ◽

10.1177/1091581820972311 ◽

2020 ◽

pp. 109158182097231

Author(s):

Lilei Zhuang ◽

Zhonghai Jin ◽

Hongguang Li ◽

Shenbao Wu ◽

Xiuping Tong ◽

...

Keyword(s):

Gut Microbiota ◽

Microbial Communities ◽

Chronic Exposure ◽

Environmental Pollutants ◽

16S Rrna Gene Sequencing ◽

Oral Route ◽

Rrna Gene ◽

Sequencing Analysis ◽

Male Mice ◽

Rrna Gene Sequencing

Increasing evidence indicates that environmental pollutants can change human gut microbiota. Microcystin–leucine arginine (MC-LR), considered a major hazard to mammals, is one of the important contaminants. However, little is known about the long-term influence of MC-LR on gut microbial communities. We aimed to investigate the effect of MC-LR on gut microbiota composition and functions by conducting a chronic exposure of male mice to MC-LR via the oral route. Using 16S rRNA gene sequencing analysis on cecum samples of mice, our results showed that significant changes of species diversity were observed in the gut microbiota of MC-LR-exposed mice. In addition, comparative analysis of the microbial communities showed that the reduction of the Actinobacteria and Saccharibacteria populations was detected in MC-LR-exposed mice. Collectively, our study highlighted the significant effects of MC-LR on the shift of gut microbial communities which could contribute to the development of metabolic syndromes.

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The gut microbiome and its potential role in paradoxical anaerobism in pupfishes of the Mojave Desert

10.21203/rs.2.19358/v2 ◽

2020 ◽

Author(s):

Shrikant S Bhute ◽

Brisa Escobedo ◽

Mina Haider ◽

Yididya Mekonen ◽

Dafhney Ferrer ◽

...

Keyword(s):

Gut Microbiota ◽

Microbial Communities ◽

Mojave Desert ◽

Critical Role ◽

16S Rrna Gene Sequencing ◽

Wide Distribution ◽

Control Group ◽

Rrna Gene ◽

Dependent Manner ◽

Bacterial Phyla

Abstract Background Pupfish frequently enter paradoxical anaerobism in response to endogenously produced or exogenously supplied ethanol in a dose-dependent manner. To decipher the role of the gut microbiota in ethanol-associated paradoxical anaerobism, gut microbial communities were depleted using a cocktail of antibiotics and profiled using 16S rRNA gene sequencing. Results Compared to the control group (n=12), microbiota-depleted fish (n=12) spent more time in paradoxical anaerobism. Our analysis indicated that the bacterial phyla Proteobacteria, Fusobacteria, Bacteroidetes, Firmicutes, Actinobacteria, Patescibacteria, and Dependentiae dominated the pupfish gut, which is consistent with other fish gut microbiota. Although the gut microbial communities with and without antibiotic treatment were similarly diverse, they were distinct and the greatest contribution to the dissimilarity (27.38%) was the common fish commensal Cetobacterium. Conclusions This study reports the first characterization of gut microbial communities of pupfish and suggests the microbiome may play a critical role in regulating metabolic strategies that are critical for survival in extremes of temperature and oxygen concentration. We speculate that Cetobacterium, a primary fermenter, also consumes ethanol through secondary fermentation via an alcohol dehydrogenase and therefore regulates the transition from paradoxical anaerobism to aerobic respiration in fish. Given the wide distribution and abundance of Cetobacterium in warm-water fishes, this process may be of broad importance, and suggests that the microbiome be carefully considered for both conservation and aquaculture.

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Effects of Alcohol Binge Drinking and Oleoylethanolamide Pretreatment in the Gut Microbiota

Frontiers in Cellular and Infection Microbiology ◽

10.3389/fcimb.2021.731910 ◽

2021 ◽

Vol 11 ◽

Author(s):

Alicia Rodríguez-González ◽

Francesco Vitali ◽

Marta Moya ◽

Carlotta De Filippo ◽

Maria Beatrice Passani ◽

...

Keyword(s):

Community Structure ◽

Gut Microbiota ◽

Binge Drinking ◽

Biological Significance ◽

16S Rrna Gene Sequencing ◽

Microbial Interactions ◽

Rrna Gene ◽

Microbiota Composition ◽

Rrna Gene Sequencing ◽

Water Administration

IntroductionChronic alcohol consumption is known to cause gut dysbiosis (changes in microbiota composition and/or function, disruptive of the normal host–microbiota interactions). However, little is known about the changes that alcohol binge drinking induces in the gut microbiota. Here, we have tested the hypothesis that a protocol of alcohol binge drinking, known to induce neuroinflammation in previous studies, also promotes intestinal dysbiosis, and we explored how oleoylethanolamide (OEA, an acylethanolamide proven to counteract alcohol binge drinking-induced neuroinflammation) pretreatment modulates alcohol-induced dysbiosis.MethodsAlcohol binges were forced by gavage three times per day during 4 consecutive days; OEA pretreatment (intraperitoneal or intragastric) was administered before each alcohol gavage. Stool microbiota composition was assessed by next-generation 16S rRNA gene sequencing, prior and after the 4-day alcohol binge protocol.ResultsAlcohol binge drinking reduced the richness of the gut microbiota and changed the microbial community, reducing Lactobacillus among other genera. Pretreatment with OEA in the alcohol-administered rats decreased the richness, evenness, and Shannon indices to a greater extent with respect to alcohol alone, also changing the community structure. Microbial interactions in the association network were further decreased following OEA administration in the alcohol group, with respect to the water administration. The synergistic interaction between alcohol binge and OEA was affected by the route of administration of OEA, since oral and i.p. administrations differently changed the community structure.ConclusionResults suggest that alcohol binge drinking produces a clear dysbiosis in animals; we observed that the well-known protective actions of OEA in the context of alcohol abuse might not be related to OEA-induced changes in alcohol-induced dysbiosis. These are observational results, and thus, further research will be needed for a complete understanding of the biological significance of the observed changes.

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Implications of Tributyrin on Gut Microbiota Shifts Related to Performances of Weaning Piglets

Microorganisms ◽

10.3390/microorganisms9030584 ◽

2021 ◽

Vol 9 (3) ◽

pp. 584

Author(s):

Francesco Miragoli ◽

Vania Patrone ◽

Aldo Prandini ◽

Samantha Sigolo ◽

Matteo Dell’Anno ◽

...

Keyword(s):

Gut Microbiota ◽

Microbial Communities ◽

Average Daily Gain ◽

Basal Diet ◽

16S Rrna Gene Sequencing ◽

Rrna Gene ◽

Microbiota Composition ◽

Growth Promoters ◽

Weaning Piglets ◽

Gut Microbiota Composition

Alternatives to antibiotic treatments are required owing to the ban on the use of these drugs as growth promoters in food animal production. Tributyrin appears to play a role in improving growth performance in pigs, albeit with varying degrees of effectiveness. So far, very little is known about its effects on gut microbiota composition. In this study, we investigated the gut microbiota changes of piglets receiving, at weaning, 0.2% tributyrin added to their basal diet. Microbiota composition was assessed through 16S-rRNA gene sequencing on stools collected from tributyrin and control groups. The functional profiles of microbial communities were predicted from amplicon abundance data. A comparison between dietary groups revealed that tributyrin strongly modulated gut microbiota composition in piglets, increasing the relative abundance of a number of bacterial genera such as Oscillospira, Oscillibacter, Mucispirillum and Butyrivibrio. These genera were positively correlated to animal average daily gain (ADG) and/or body weight (BW). Based on the function profile prediction, the gut microbiome of the tributyrin group possessed an enhanced potential for energy metabolism and a reduced potential for carbohydrate metabolism. In conclusion, our results indicated that tributyrin can promote changes to gut microbial communities, which could contribute to improving animal performance after weaning.

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Characteristic gut microbiota and predicted metabolic functions in women with PCOS

Endocrine Connections ◽

10.1530/ec-19-0522 ◽

2020 ◽

Vol 9 (1) ◽

pp. 63-73 ◽

Cited By ~ 9

Author(s):

Ling Zhou ◽

Zhexin Ni ◽

Wen Cheng ◽

Jin Yu ◽

Shuai Sun ◽

...

Keyword(s):

Gut Microbiota ◽

Polycystic Ovary ◽

16S Rrna Gene Sequencing ◽

Cross Sectional Study ◽

Rrna Gene ◽

Obese Women ◽

Cross Sectional ◽

Linear Discriminant ◽

Metabolic Functions ◽

And Function

Polycystic ovary syndrome (PCOS) is a chronic endocrine and metabolic disease. Gut microbiota is closely related to many chronic diseases. In this study, we conducted a cross-sectional study and recruited 30 obese (OG) and 30 non-obese (NG) women with PCOS, 30 healthy women (NC) and 11 healthy but obese women (OC) as controls to investigate the characteristic gut microbiota and its metabolic functions in obese and non-obese patients with PCOS. The blood and non-menstrual faecal samples of all the participants were collected and analysed. As a result, the Hirsutism score, LH/FSH and serum T level in NG and OG both increased significantly compared with their controls (P < 0.05). High-throughput 16S rRNA gene sequencing revealed that the abundance and diversity of the gut microbiota changed in patients with PCOS. The linear discriminant analysis (LDA) indicated that Lactococcus was the characteristic gut microbiota in NG, while Coprococcus_2 in OG. Correlation heatmap analysis revealed that the sex hormones and insulin levels in human serum were closely related to the changes in the gut microbiota of NG and OG. Functional prediction analysis demonstrated that the citrate cycle pathway enriched both in NG and OG, and other 12 gut bacterial metabolic pathways enriched in NG. This study highlighted significant differences in the gut microbiota and predictive functions of obese and non-obese women with PCOS, thereby providing insights into the role and function of the gut microbiota that may contribute to the occurrence and development of PCOS in obese and non-obese women.

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Differences in the Gut Microbiomes of Distinct Ethnicities Within the Same Geographic Area Are Linked to Host Metabolic Health

10.21203/rs.3.rs-100084/v1 ◽

2020 ◽

Author(s):

Qi Yan Ang ◽

Diana L Alba ◽

Vaibhav Upadhyay ◽

Jordan E. Bisanz ◽

Jingwei Cai ◽

...

Keyword(s):

Community Structure ◽

Gut Microbiota ◽

Dietary Intake ◽

Ethnic Groups ◽

San Francisco ◽

Geographical Location ◽

16S Rrna Gene Sequencing ◽

Geographic Area ◽

Rrna Gene ◽

Starting Point

Abstract BackgroundThe human gut microbiota exhibits marked variation around the world, which has been attributed to dietary intake and other environmental factors. However, the degree to which ethnicity-associated differences in gut microbial community structure and function are maintained following immigration or in the context of metabolic disease is poorly understood.ResultsWe conducted a multi-omic study of 46 lean and obese East Asian (EA) and White (W) participants living in the San Francisco Bay Area. 16S rRNA gene sequencing revealed significant differences between ethnic groups in bacterial richness and community structure. W individuals were enriched for the mucin-degrading Akkermansia muciniphila. EA participants had increased levels of multiple Bacteroidetes species, fermentative pathways detected by metagenomics, and the short-chain fatty acid end products acetate, propionate, and isobutyrate. Differences in the gut microbiota between the EA and W groups could not be explained by reported dietary intake, were more pronounced for lean individuals, and were associated with current geographical location. Microbiome transplantations into germ-free mice confirmed that the differences in the gut microbiota of the EA and W individuals we analyzed are indeed independent of diet and that they differentially impact host body weight and adiposity in genetically identical mouse recipients.ConclusionsThe reported findings emphasize the utility of studying diverse ethnic groups within a defined geographical location and provide a starting point for dissecting the mechanisms contributing to the complex interactions between the gut microbiome and ethnicity-associated lifestyle, demographic, metabolic, and genetic factors.

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Diversity, Composition and Functional Inference of Gut Microbiota in Indian Cabbage white Pieris canidia (Lepidoptera: Pieridae)

Life ◽

10.3390/life10110254 ◽

2020 ◽

Vol 10 (11) ◽

pp. 254

Author(s):

Ying Wang ◽

Jianqing Zhu ◽

Jie Fang ◽

Li Shen ◽

Shuojia Ma ◽

...

Keyword(s):

Gut Microbiota ◽

16S Rrna Gene Sequencing ◽

Adult Survival ◽

Rrna Gene ◽

Life Stages ◽

Microbial Composition ◽

Significant Difference ◽

Functional Inference ◽

Rrna Gene Sequencing ◽

Insect Fitness

We characterized the gut microbial composition and relative abundance of gut bacteria in the larvae and adults of Pieris canidia by 16S rRNA gene sequencing. The gut microbiota structure was similar across the life stages and sexes. The comparative functional analysis on P. canidia bacterial communities with PICRUSt showed the enrichment of several pathways including those for energy metabolism, immune system, digestive system, xenobiotics biodegradation, transport, cell growth and death. The parameters often used as a proxy of insect fitness (development time, pupation rate, emergence rate, adult survival rate and weight of 5th instars larvae) showed a significant difference between treatment group and untreated group and point to potential fitness advantages with the gut microbiomes in P. canidia. These data provide an overall view of the bacterial community across the life stages and sexes in P. canidia.

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Gut microbiota markers associated with obesity and overweight in Italian adults

Scientific Reports ◽

10.1038/s41598-021-84928-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Vanessa Palmas ◽

Silvia Pisanu ◽

Veronica Madau ◽

Emanuela Casula ◽

Andrea Deledda ◽

...

Keyword(s):

Gut Microbiota ◽

Relative Abundance ◽

Smoking Status ◽

16S Rrna Gene Sequencing ◽

Normal Weight ◽

Activity Level ◽

Rrna Gene ◽

Illumina Platform ◽

Obesity And Overweight ◽

Rrna Gene Sequencing

AbstractIn the present study, we characterized the distinctive signatures of the gut microbiota (GM) from overweight/obese patients (OB), and normal-weight controls (NW), both of Sardinian origin. Fecal bacterial composition of 46 OB patients (BMI = 36.6 ± 6.0; F/M = 40/6) was analyzed and compared to that of 46 NW subjects (BMI = 21.6 ± 2.1; F/M = 41/5), matched for sex, age and smoking status, by using 16S rRNA gene sequencing on MiSeq Illumina platform. The gut microbial community of OB patients exhibited a significant decrease in the relative abundance of several Bacteroidetes taxa (i.e. Flavobacteriaceae, Porphyromonadaceae, Sphingobacteriaceae, Flavobacterium, Rikenella spp., Pedobacter spp., Parabacteroides spp., Bacteroides spp.) when compared to NW; instead, several Firmicutes taxa were significantly increased in the same subjects (Lachnospiraceae, Gemellaceae, Paenibacillaceae, Streptococcaceae, Thermicanaceae, Gemella, Mitsuokella, Streptococcus, Acidaminococcus spp., Eubacterium spp., Ruminococcus spp., Megamonas spp., Streptococcus, Thermicanus, Megasphaera spp. and Veillonella spp.). Correlation analysis indicated that body fatness and waist circumference negatively correlated with Bacteroidetes taxa, while Firmicutes taxa positively correlated with body fat and negatively with muscle mass and/or physical activity level. Furthermore, the relative abundance of several bacterial taxa belonging to Enterobacteriaceae family, known to exhibit endotoxic activity, was increased in the OB group compared to NW. The results extend our knowledge on the GM profiles in Italian OB, identifying novel taxa linking obesity and intestine.

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