scholarly journals Antibacterial Properties and Cytotoxicity of 100% Waste Derived Alkali Activated Materials: Slags and Stone Wool-Based Binders

2021 ◽  
Vol 8 ◽  
Author(s):  
Caterina Sgarlata ◽  
Giovanni Dal Poggetto ◽  
Federica Piccolo ◽  
Michelina Catauro ◽  
Katja Traven ◽  
...  
Keyword(s):  
Ecological Impact ◽  
Antibacterial Properties ◽  
Mouse Embryonic Fibroblast ◽  
Bacterial Strains ◽  
Bacterial Colony ◽  
E Coli ◽  
Heavy Metal Cations ◽  
Stone Wool ◽  
Nih 3T3 ◽  
Alkali Activated

In this study we compare the leaching behavior and the antibacterial and cytotoxic properties of 100% slag or stone wool derived alkali activated materials. The antibacterial activity was measured as the inhibiting capacity against two Gram-negative bacterial strains, Escherichia coli and Pseudomonas aeruginosa and one Gram-positive bacterial strain: Enterococcus faecalis. The cytotoxicity properties were tested on mouse embryonic fibroblast NIH-3T3 cell-line. It was proved that the high quality of the 3D aluminosilicate network of the consolidated materials obtained from powders of CaO or MgO-rich slags or stone wool, opportunely activated with NaO and/or Na-silicate, was capable of stabilizing heavy metal cations. The concentrations of leachate heavy cations were lower than the European law limit when tested in water. The effect of additives in the composites, basal fibers or nanocellulose, did not reduce the chemical stability and slightly influenced the compressive strength. Weight loss in water increased by 20% with basalt fibers addition, while it remained almost constant when nanocellulose was added. All the consolidated materials, cement-like in appearance, exhibited limited antibacterial properties (viability from 50 to 80% depending on the bacterial colony and the amount of sample) and absence of cytotoxicity, envisaging good acceptance from part of the final consumer and zero ecological impact. CaO-rich formulations can replace ordinary Portland cement (showing bacterial viability at 100%) with a certain capability for preventing the reproduction of the E. coli and S. aureus bacteria with health and environmental protection results.

scholarly journals Decoding Antibacterial and Antibiofilm Properties of Cinnamon and Cardamom Essential Oils: A Combined Molecular Docking and Experimental Study

2021 ◽  
Author(s):  
Elahe Pourkhosravani ◽  
fatemeh dehghan nayeri ◽  
Mitra Mohammadi Bazargani
Keyword(s):  
Cell Attachment ◽  
Antibacterial Properties ◽  
Binding Mode ◽  
Dilution Method ◽  
Antibiofilm Activity ◽  
Bacterial Strains ◽  
Biofilm Growth ◽  
Computational Docking ◽  
E Coli ◽  
Industrial Strains

Abstract This study sets out to compare the antibacterial and antibiofilm profiles of Ci/Ca EOs alone and in combination together against infectious bacterial strains. MIC assay was carried out to survey the effectiveness of prepared EOs by two-fold serial dilution method and MTT evaluation. Synergic antibacterial properties of EOs against target strains were studied by using checkerboard titration method. Biofilm growth and development were evaluated using CV and XTT reduction assays. Antibacterial activity was observed for EOs against both bacterial strains with stronger activity for CiEO against both bacteria. The synergistic antibacterial effect was observed only against B. subtilis. Based on the FIC index, combinations could not inhibit the growth of E. coli. The pure EOs and their combination inhibited cell attachment for both studied bacteria with stronger effect on E. coli. CV and XTT reduction assays results showed that Ci EO and its combination with CaEO had the highest antibiofilm activity at lowest MIC value 0.08% and 0.04/0.02% against biofilm formed by E. coli and B. subtilis respectively, indicating a high antibiofilm potential. Computational docking analyses also postulated that the active constituents of evaluated EOs have the potential to interact with different bacterial targets, suggested binding mode of action of EOs metabolites. By and large, synergistic anti-biofilm properties of EOs may provide further options for developing novel formula to inhibit a variety of infectious clinical and industrial strains without (or less) toxicity effects on human body.

scholarly journals Phytochemical Study and Antibacterial and Antibiotic Modulation Activity of Punica granatum (Pomegranate) Leaves

Scientifica ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Amine Trabelsi ◽  
Mohamed Amine El Kaibi ◽  
Aïmen Abbassi ◽  
Amira Horchani ◽  
Leila Chekir-Ghedira ◽  
...  
Keyword(s):  
Antibacterial Properties ◽  
Punica Granatum ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Phytochemical Study ◽  
E Coli ◽  
Microdilution Method ◽  
Resistant Strains

This study aimed to determine phytochemical contents, antibacterial properties, and antibiotic modulating potential of Punica granatum leaf extracts: hexane, chloroform, ethyl acetate, ethanol, and aqueous extracts as well as an extract enriched with total oligomer flavonoids (TOFs). The TOF extract contained the highest value of phenols and flavonoids. Rutin, luteolin, gallic acid, and ellagic acid were determined by HPLC analysis of this extract. The antibacterial activity was assayed by the disc diffusion method and microdilution method against Staphylococcus aureus and Escherichia coli standard ATCC strains and clinical isolates resistant strains. The TOF extract was the most active against all tested strains. The checkerboard method was used for the determination of synergy between two antibiotics (amoxicillin and cefotaxime) and P. granatum leaf extracts. The best synergistic interaction was found with TOF extract combined with amoxicillin for penicillin-resistant E. coli and penicillin-resistant S. aureus. These results can be assigned to tannins, flavonoids, and phenolic acids found in P. granatum leaf extracts. Pomegranate leaf extracts or active compounds isolated from these extracts could be used to fight the emergence and spread of resistant bacterial strains.

scholarly journals Chitosan & Conductive PANI/Chitosan Composite Nanofibers - Evaluation of Antibacterial Properties

2019 ◽  
Vol 4 (1) ◽  
pp. 6-20 ◽  
Author(s):  
Panagiota Moutsatsou ◽  
Karen Coopman ◽  
Stella Georgiadou
Keyword(s):  
Chronic Wounds ◽  
Antibacterial Properties ◽  
Wound Dressings ◽  
Bacterial Strains ◽  
Healthcare Applications ◽  
E Coli ◽  
Jet Stability ◽  
Electrospun Membranes ◽  
New Treatments ◽  
Chitosan Composite

Background: Within the healthcare industry, including the care of chronic wounds, the challenge of antimicrobial resistance continues to grow. As such, there is a need to develop new treatments that can reduce the bioburden in wounds. Objective: The present study is focused on the development of polyaniline (PANI) / chitosan (CH) nanofibrous electrospun membranes and evaluates their antibacterial properties. Methods: To this end, experimental design was used to determine the electrospinning windows of both pure chitosan and PANI/CH blends of different ratios (1:3, 3:5, 1:1). The effect of key environmental and process parameters (relative humidity and applied voltage) was determined, as well as the effect of the PANI/CH ratio in the blend and the molecular interactions between PANI and chitosan that led to jet stability. Results: The nanofibrous mats were evaluated regarding their morphology and antibacterial effect against model gram positive and gram negative bacterial strains, namely B. subtilis and E. coli. High PANI content mats show increased bactericidal activity against both bacterial strains. Conclusion: The blend fibre membranes combine the materials’ respective properties, namely electrical conductivity, biocompatibility and antibacterial activity. This study suggests that electrospun PANI/CH membranes are promising candidates for healthcare applications, such as wound dressings.

scholarly journals Antibacterial effect of TiO2 modified with poly-amidoamine dendrimer – G3 on S. aureus and E. coli in aqueous solutions

2022 ◽  
Author(s):  
Soudabeh Alizadeh Matboo ◽  
Shahram Nazari ◽  
Ali Niapour ◽  
Mehdi Vosoughi Niri ◽  
Esrafil Asgari ◽  
...  
Keyword(s):  
Inhibitory Concentration ◽  
Antibacterial Properties ◽  
Plate Count ◽  
Bacterial Strains ◽  
Solution Ph ◽  
E Coli ◽  
Bacterial Removal ◽  
Mic Minimum Inhibitory Concentration ◽  
Paper Disc ◽  
Surface Plate

Abstract This study investigated the bacterial removal using TiO2 nanoparticles (NPs) modified with poly-amidoamine dendrimer macromolecule (PAMAM, G3). The PAMAM G3/TiO2 (nanohybrid) was used to specify antibacterial properties via broth microdilution (MBC-Minimum Bactericidal Concentration and MIC-Minimum Inhibitory Concentration- determination), paper disc diffusion, and surface plate count methods. The nanohybrid was characterized via the different techniques. The effects of different factors including initial bacteria count, run time, solution pH, and the nanohybrid concentration were studied. The nanohybrid cytotoxicity was studied on AGS and MKN45 cells line by MTT assay. It was revealed that the nanohybrid was effective in intercepting both bacterial strains growth. The MIC value for S. aureus and E. coli were determined to be 4 and 2 μg/mL, respectively. The MBC value for both strains were calculated to be 32 μg/mL. The results showed removal efficiency of 100% for S. aureus and E. coli bacteria in optimum situation. The decrease in cell viability in the dosage of 32 μg/mL after 72 h treatment for AGS and MKN45 cells line were shown to be 6.2 and 4.6%, respectively. The nanohybrid was able to decrease the S. aureus and E. coli count in solution, which meets the drinking water criterions aligned with WHO guidelines.

scholarly journals Atmospheric Pressure Microplasma for Antibacterial Silver Nanoparticle/Chitosan Nanocomposites With Tailored Properties

2019 ◽  
Author(s):  
Daye Sun ◽  
Jonathan Turner ◽  
Nan Jiang ◽  
Songsong Zhu ◽  
Li Zhang ◽  
...  
Keyword(s):  
Silver Nanoparticle ◽  
Atmospheric Pressure ◽  
Room Temperature ◽  
Polymer Network ◽  
Antibacterial Properties ◽  
Bacterial Strains ◽  
E Coli ◽  
Modified Chitosan ◽  
First Time

<p>Room temperature atmospheric pressure microplasma (APM) was deployed for the first time for the in situ synthesis of anti-bacterial silver nanoparticle / chitosan (AgNP/CS) nanocomposites. The plasma induced liquid chemistry plays a role in the in situ formation of AgNP, the size distribution of which depends on the silver salt precursor concentration. The microplasma process has also simultaneously tailored the physical properties of the composites, rendering more crosslinked chitosan polymer network with shorter molecular chains. The formation of AgNP within the <i>in situ</i> modified chitosan has led to nanocomposites with overall improved mechanical properties and better stability in simulated body fluid. Our plasma synthesized AgNP/CS nanocomposites also demonstrate effective antibacterial properties against <i>E. Coli</i> and <i>S. Aureus</i> bacterial strains, showing their promise in potential antimicrobial applications.</p>

Evaluation of Biological Activities of Chemically Synthesized Cobalt Oxide Nanoparticles in Concentration and Time Dependent Manner

2020 ◽  
Vol 13 (6) ◽  
pp. 5243-5249
Author(s):  
Vijayta Gupta ◽  
Vinay Kant ◽  
Meena Sharma
Keyword(s):  
Free Radicals ◽  
Cobalt Oxide ◽  
Biological Activities ◽  
Antibacterial Properties ◽  
Co3o4 Nanoparticles ◽  
Dependent Manner ◽  
Bacterial Strains ◽  
Concentration Dependent Manner ◽  
E Coli

The promising results of metal oxides nanoparticles in different areas including the biological system lead us to investigate the antioxidant and antimicrobial actions of chemically synthesized cobalt oxide (Co3O4) nanoparticles. The different concentrations of synthesized Co3O4 nanoparticles were prepared and evaluated for different parameters at different time intervals i.e.  on day 1, 30 and 60 after preparations.  Co3O4 nanoparticles synthesized in this study were of 52.2 nm average size with a polydispersity index of 0.465. We observed that Co3O4 nanoparticles scavenge different in vitro free radicals (DPPH, ABTS, superoxide anion and hydrogen peroxide radicals) in concentration dependent manner. The percentage of inhibitions of free radicals by Co3O4 nanoparticles was markedly more on day 1 as compared to day 30 and 60. The IC50 values of Co3O4 nanoparticles for these free radicals were also on day 1 as compared to day 30 and 60. The Co3O4 nanoparticles showed the antibacterial actions against both the bacterial strains i.e. S. aureus and E. coli. The MIC and MBC values revealed that action of Co3O4 nanoparticles was more against E. coli than S. aureus. The MIC and MBC values were lower on day 1 as compared to day 30 and 60 with respective to specific bacteria. In conclusions, the Co3O4 nanoparticles synthesized in this study showed potent antioxidant and antibacterial properties due to which it may serve as promising candidate for the combat the biological problems humans, animals and plants associated with reactive oxygen species and bacteria.

scholarly journals Gram-scale synthesis of splat-shaped Ag–TiO2 nanocomposites for enhanced antimicrobial properties

2020 ◽  
Vol 11 ◽  
pp. 1119-1125
Author(s):  
Mohammad Jaber ◽  
Asim Mushtaq ◽  
Kebiao Zhang ◽  
Jindan Wu ◽  
Dandan Luo ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Multidrug Resistant ◽  
Bacterial Strains ◽  
Gram Negative ◽  
E Coli ◽  
Contagious Diseases ◽  
Good Biocompatibility

The control over contagious diseases caused by pathogenic organisms has become a serious health issue. The extensive usage of antibiotics has led to the development of multidrug-resistant bacterial strains. In this regard, metal-oxide-based antibacterial nanomaterials have received potential research interest due to the efficient prevention of microorganism growth. In this study, splat-shaped Ag–TiO2 nanocomposites (NCs) were synthesized on the gram scale and the enhanced antibacterial properties of TiO2 in the presence of silver were examined. The formation of Ag–TiO2 NCs was analyzed through various characterization techniques. The cell viability experimental results demonstrated that the Ag–TiO2 NCs have good biocompatibility. The antibacterial activity of the prepared Ag–TiO2 NCs was tested against the Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) bacterial strains. The Ag–TiO2 NCs exhibited promising and superior antibacterial properties compared to TiO2 nanospheres as confirmed by the bacterial growth and inhibition zone. The improvement in the antibacterial activity was attributed to the synergistic effect of the hybrid nature of TiO2 nanoparticles in the presence of Ag.

scholarly journals Development and Characterization of Active Gelatin Films Loaded with Rapeseed Meal Extracts

Materials ◽  
2021 ◽  
Vol 14 (11) ◽  
pp. 2869
Author(s):  
Alicja Tymczewska ◽  
Bliss Ursula Furtado ◽  
Jacek Nowaczyk ◽  
Katarzyna Hrynkiewicz ◽  
Aleksandra Szydłowska-Czerniak
Keyword(s):  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Rapeseed Meal ◽  
Active Packaging ◽  
Bacterial Strains ◽  
Casting Technique ◽  
E Coli ◽  
Industry Waste ◽  
Gelatin Films ◽  
Radical Scavenging Properties

The use of industrial waste as a material for the development of natural innovative and active packaging is economically and environmentally appealing. The aim of this study was to develop and characterize active gelatin films incorporating rapeseed oil industry waste. Water (RM-WE) and methanolic (RM-MWE) extracts of rapeseed meal (RM) were used as active agents in film formulations. The active films were produced by a casting technique. The physicochemical, mechanical, optical, morphological, radical scavenging, and antibacterial properties of the films were analyzed. The addition of RM-WE and RM-MWE in the concentrations range between 4 and 12% promoted an increase of Young’s modulus (YM) and radical scavenging properties of films investigated by the direct QUick, Easy, New, CHEap and Reproducible procedure using 2,2-diphenyl-1-picrylhydrazyl (QUENCHERDPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6- sulfonic acid) (QUENCHERABTS) radicals. The antibacterial properties of films were examined against five bacterial strains: E. coli, S. enterica, M. luteus, L. monocytogenes, and S. aureus. Additionally, color and opacity of the control and fortified films differed significantly. The gelatin films with RM extracts are resistant to the microbial spoilage and could be used to produce active packaging for food that is vulnerable to rancidity effects.

scholarly journals Atmospheric Pressure Microplasma for Antibacterial Silver Nanoparticle/Chitosan Nanocomposites With Tailored Properties

2019 ◽  
Author(s):  
Daye Sun ◽  
Jonathan Turner ◽  
Nan Jiang ◽  
Songsong Zhu ◽  
Li Zhang ◽  
...  
Keyword(s):  
Silver Nanoparticle ◽  
Atmospheric Pressure ◽  
Room Temperature ◽  
Polymer Network ◽  
Antibacterial Properties ◽  
Bacterial Strains ◽  
E Coli ◽  
Modified Chitosan ◽  
First Time

<p>Room temperature atmospheric pressure microplasma (APM) was deployed for the first time for the in situ synthesis of anti-bacterial silver nanoparticle / chitosan (AgNP/CS) nanocomposites. The plasma induced liquid chemistry plays a role in the in situ formation of AgNP, the size distribution of which depends on the silver salt precursor concentration. The microplasma process has also simultaneously tailored the physical properties of the composites, rendering more crosslinked chitosan polymer network with shorter molecular chains. The formation of AgNP within the <i>in situ</i> modified chitosan has led to nanocomposites with overall improved mechanical properties and better stability in simulated body fluid. Our plasma synthesized AgNP/CS nanocomposites also demonstrate effective antibacterial properties against <i>E. Coli</i> and <i>S. Aureus</i> bacterial strains, showing their promise in potential antimicrobial applications.</p>

scholarly journals Antibacterial Effect of Plant Resin Collected from Tetrigona apicalis (Smith, 1857) in Thung Salaeng Luang National Park, Phitsanulok

2017 ◽  
Vol 15 (8) ◽  
pp. 599-607
Author(s):  
Sathirapong KRAIKONGJIT ◽  
Touchkanin JONGJITVIMOL ◽  
Naklao MIANJINDA ◽  
Nutta SIRITHEP ◽  
Thodsaporn KAEWBOR ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
National Park ◽  
Stingless Bees ◽  
Antibacterial Properties ◽  
Total Phenolic ◽  
Bacterial Cells ◽  
Bacterial Strains ◽  
E Coli ◽  
Phenolic Contents ◽  
Two Samples

Tetrigona apicalis (Smith, 1857) is a common species of stingless bee found in lower northern Thailand. In previous studies, the propolis of stingless bees has been shown to have antibacterial properties, due to its chemically contained phenolic contents. The major component of propolis is resin. The purpose of this study, therefore, was to evaluate the antibacterial activities of crude resin extracts by disk diffusion and broth microdilution methods. We also determined the total phenolic contents using the Folin-Ciocalteau method and, to detect individual polyphenolic contents, we used the high performance liquid chromatographic method. Two samples of resin were collected from Thung Salaeng Luang National Park, Phitsanulok. The first sample was from fresh plants, which stingless bees used for nest construction. The second sample was taken from entrances of the bee’s nest. All samples were macerated in 30 % ethanol and incubated at room temperature for 14 days. The supernatants were filtered and ethanol residues then removed as ethanolic resin extracts (eREs). The antibacterial activity of the extracted resins against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853 was examined. The disks containing 9 and 14 mg of eREs produced obvious inhibition zones against S. aureus, but did not show zones against E. coli and P. aeruginosa. The minimum inhibitory concentrations (MICs) of the eREs against the bacterial strains tested were variously between 6 and 48 mg/ml, whereas the minimum bactericidal concentrations (MBCs) were from 12 to 48 mg/ml. The amount of the total phenolic compounds in the eREs from the fresh resin was 9,908 mg of pyrogallol equivalent (PGE) per kg of eREs, and from the nest entrances, 14,740 mg per kg. We also found that hydroquinin had the highest concentration in both extracts. In conclusion, the crude resin extracts demonstrated antibacterial properties against the S. aureus, E. coli, and P. aeruginosa strains tested. They also contained phenolic compounds which were active antibacterial agents. We have identified new and novel knowledge which can be used as preliminary data, leading to further, more detailed, investigation of the mechanistic action of the resin against bacterial cells.

Sign in / Sign up

Export Citation Format

Share Document