Antibacterial effect of TiO2 modified with poly-amidoamine dendrimer – G3 on S. aureus and E. coli in aqueous solutions

Abstract This study investigated the bacterial removal using TiO2 nanoparticles (NPs) modified with poly-amidoamine dendrimer macromolecule (PAMAM, G3). The PAMAM G3/TiO2 (nanohybrid) was used to specify antibacterial properties via broth microdilution (MBC-Minimum Bactericidal Concentration and MIC-Minimum Inhibitory Concentration- determination), paper disc diffusion, and surface plate count methods. The nanohybrid was characterized via the different techniques. The effects of different factors including initial bacteria count, run time, solution pH, and the nanohybrid concentration were studied. The nanohybrid cytotoxicity was studied on AGS and MKN45 cells line by MTT assay. It was revealed that the nanohybrid was effective in intercepting both bacterial strains growth. The MIC value for S. aureus and E. coli were determined to be 4 and 2 μg/mL, respectively. The MBC value for both strains were calculated to be 32 μg/mL. The results showed removal efficiency of 100% for S. aureus and E. coli bacteria in optimum situation. The decrease in cell viability in the dosage of 32 μg/mL after 72 h treatment for AGS and MKN45 cells line were shown to be 6.2 and 4.6%, respectively. The nanohybrid was able to decrease the S. aureus and E. coli count in solution, which meets the drinking water criterions aligned with WHO guidelines.

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AKTIVITAS ANTIBAKTERI EKSTRAK BIJI JERUK SIAM (Citrus reticulata) PADA BAKTERI Escherichia coli

Jurnal Bioteknologi & Biosains Indonesia (JBBI) ◽

10.29122/jbbi.v7i2.4194 ◽

2020 ◽

Vol 7 (2) ◽

pp. 289-295

Author(s):

Mohammad Arfi Setiawan ◽

Mita Dewi Retnoningrum ◽

Febriyandhi Yahya ◽

Resa Ragil Andika ◽

Dyan Hatining Ayu Sudarni

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Minimum Inhibitory Concentration ◽

Inhibitory Concentration ◽

Seed Extract ◽

Nutrient Agar ◽

Citrus Reticulata ◽

E Coli ◽

Mic Minimum Inhibitory Concentration ◽

Paper Disc

Antibacterial Activity of Citrus seed (Citrus reticulata) Extract on Escherichia coli Indonesian agriculture provides a resource of medicinal plants whose potential needs to be explored in order to benefit society. One of them is the use of Siam orange seeds (Citrus reticulata) which has the potential for the production of antibacterial compounds. This study aims to test the antibacterial activity of the ethanol and n-hexane extract of orange seeds. The extract was obtained through maceration techniques using ethanol and n-hexane as solvents. The antibacterial activity test of orange seeds against Escherichia coli used the paper disc diffusion method with nutrient agar (NA) media. The concentration of orange seed extract for the determination of MIC (Minimum Inhibitory Concentration) was 0.5, 2, 8, 10, 20 mg mL-1. The results showed that the ethanol and n-hexane extract of orange seeds had antibacterial activity against E. coli. However, the ethanol extract had a higher antibacterial effect than the n-hexane orange seed extract. From the results of this study, it is hoped that the waste of orange seeds will provide beneficial contribution for pharmaceutical development. Pertanian Indonesia memiliki sumber tanaman obat yang perlu digali potensinya agar bermanfaat bagi masyarakat. Salah satunya pemanfaatan biji jeruk siam (Citrus reticulata) yang berpotensi menghasilkan senyawa antibakteri. Penelitian ini bertujuan untuk menguji aktivitas antibakteri ekstrak etanol dan n-heksana biji jeruk. Ekstrak diperoleh melalui teknik maserasi menggunakan pelarut etanol dan n-heksana. Uji aktivitas antibakteri biji jeruk terhadap Escherichia coli menggunakan metode difusi paper disc dengan media nutrient agar (NA). Konsentrasi ekstrak biji jeruk untuk penentuan MIC (Minimum Inhibitory Concentration) adalah 0,5, 2, 8, 10, 20 mg mL-1. Hasil penelitian menunjukkan bahwa ekstrak etanol dan n-heksana biji jeruk memiliki aktivitas antibakteri terhadap E. coli. Namun, ekstrak etanol memiliki efek antibakteri yang lebih tinggi dibandingkan ekstrak biji jeruk n-heksana. Dari hasil penelitian ini, limbah biji jeruk diharapkan dapat memberikan kontribusi bermanfaat bagi pengembangan farmasi.

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Decoding Antibacterial and Antibiofilm Properties of Cinnamon and Cardamom Essential Oils: A Combined Molecular Docking and Experimental Study

10.21203/rs.3.rs-955241/v1 ◽

2021 ◽

Author(s):

Elahe Pourkhosravani ◽

fatemeh dehghan nayeri ◽

Mitra Mohammadi Bazargani

Keyword(s):

Cell Attachment ◽

Antibacterial Properties ◽

Binding Mode ◽

Dilution Method ◽

Antibiofilm Activity ◽

Bacterial Strains ◽

Biofilm Growth ◽

Computational Docking ◽

E Coli ◽

Industrial Strains

Abstract This study sets out to compare the antibacterial and antibiofilm profiles of Ci/Ca EOs alone and in combination together against infectious bacterial strains. MIC assay was carried out to survey the effectiveness of prepared EOs by two-fold serial dilution method and MTT evaluation. Synergic antibacterial properties of EOs against target strains were studied by using checkerboard titration method. Biofilm growth and development were evaluated using CV and XTT reduction assays. Antibacterial activity was observed for EOs against both bacterial strains with stronger activity for CiEO against both bacteria. The synergistic antibacterial effect was observed only against B. subtilis. Based on the FIC index, combinations could not inhibit the growth of E. coli. The pure EOs and their combination inhibited cell attachment for both studied bacteria with stronger effect on E. coli. CV and XTT reduction assays results showed that Ci EO and its combination with CaEO had the highest antibiofilm activity at lowest MIC value 0.08% and 0.04/0.02% against biofilm formed by E. coli and B. subtilis respectively, indicating a high antibiofilm potential. Computational docking analyses also postulated that the active constituents of evaluated EOs have the potential to interact with different bacterial targets, suggested binding mode of action of EOs metabolites. By and large, synergistic anti-biofilm properties of EOs may provide further options for developing novel formula to inhibit a variety of infectious clinical and industrial strains without (or less) toxicity effects on human body.

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Phytochemical Study and Antibacterial and Antibiotic Modulation Activity of Punica granatum (Pomegranate) Leaves

Scientifica ◽

10.1155/2020/8271203 ◽

2020 ◽

Vol 2020 ◽

pp. 1-7

Author(s):

Amine Trabelsi ◽

Mohamed Amine El Kaibi ◽

Aïmen Abbassi ◽

Amira Horchani ◽

Leila Chekir-Ghedira ◽

...

Keyword(s):

Antibacterial Properties ◽

Punica Granatum ◽

Diffusion Method ◽

Bacterial Strains ◽

Leaf Extracts ◽

Phytochemical Study ◽

E Coli ◽

Microdilution Method ◽

Resistant Strains

This study aimed to determine phytochemical contents, antibacterial properties, and antibiotic modulating potential of Punica granatum leaf extracts: hexane, chloroform, ethyl acetate, ethanol, and aqueous extracts as well as an extract enriched with total oligomer flavonoids (TOFs). The TOF extract contained the highest value of phenols and flavonoids. Rutin, luteolin, gallic acid, and ellagic acid were determined by HPLC analysis of this extract. The antibacterial activity was assayed by the disc diffusion method and microdilution method against Staphylococcus aureus and Escherichia coli standard ATCC strains and clinical isolates resistant strains. The TOF extract was the most active against all tested strains. The checkerboard method was used for the determination of synergy between two antibiotics (amoxicillin and cefotaxime) and P. granatum leaf extracts. The best synergistic interaction was found with TOF extract combined with amoxicillin for penicillin-resistant E. coli and penicillin-resistant S. aureus. These results can be assigned to tannins, flavonoids, and phenolic acids found in P. granatum leaf extracts. Pomegranate leaf extracts or active compounds isolated from these extracts could be used to fight the emergence and spread of resistant bacterial strains.

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Chitosan & Conductive PANI/Chitosan Composite Nanofibers - Evaluation of Antibacterial Properties

Current Nanomaterials ◽

10.2174/1573413714666181114110651 ◽

2019 ◽

Vol 4 (1) ◽

pp. 6-20 ◽

Cited By ~ 5

Author(s):

Panagiota Moutsatsou ◽

Karen Coopman ◽

Stella Georgiadou

Keyword(s):

Chronic Wounds ◽

Antibacterial Properties ◽

Wound Dressings ◽

Bacterial Strains ◽

Healthcare Applications ◽

E Coli ◽

Jet Stability ◽

Electrospun Membranes ◽

New Treatments ◽

Chitosan Composite

Background: Within the healthcare industry, including the care of chronic wounds, the challenge of antimicrobial resistance continues to grow. As such, there is a need to develop new treatments that can reduce the bioburden in wounds. Objective: The present study is focused on the development of polyaniline (PANI) / chitosan (CH) nanofibrous electrospun membranes and evaluates their antibacterial properties. Methods: To this end, experimental design was used to determine the electrospinning windows of both pure chitosan and PANI/CH blends of different ratios (1:3, 3:5, 1:1). The effect of key environmental and process parameters (relative humidity and applied voltage) was determined, as well as the effect of the PANI/CH ratio in the blend and the molecular interactions between PANI and chitosan that led to jet stability. Results: The nanofibrous mats were evaluated regarding their morphology and antibacterial effect against model gram positive and gram negative bacterial strains, namely B. subtilis and E. coli. High PANI content mats show increased bactericidal activity against both bacterial strains. Conclusion: The blend fibre membranes combine the materials’ respective properties, namely electrical conductivity, biocompatibility and antibacterial activity. This study suggests that electrospun PANI/CH membranes are promising candidates for healthcare applications, such as wound dressings.

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Isolation and Characterization of Bacterium isolated from Bantala Tannery Solid Wastes

NASS Journal of Agricultural Sciences ◽

10.36956/njas.v1i1.9 ◽

2019 ◽

Vol 1 (1) ◽

Author(s):

Anannya Banerjee ◽

Sudipta Tripathi

Keyword(s):

Inhibitory Concentration ◽

West Bengal ◽

Accession Number ◽

Bacterial Strains ◽

X Ray ◽

Isolation And Characterization ◽

Chrome Tanning ◽

Tanning Process ◽

Mic Minimum Inhibitory Concentration

Leather industries that uses the conventional chrome tanning process are subjected to high risk of contamination due to the emission of toxic Cr(VI) that poses a serious threat to the environment and human’s wellbeing. The present study were made to isolate and characterize chromium tolerant bacteria in the samples collected from four different plots of Bantala Tannery, Kolkata, West Bengal, India. Pure chromium tolerant bacterial strains were isolated from the tannery sludge samples and their relative MIC (Minimum Inhibitory Concentration) were recorded at different concentrations of Cr (VI) salts to select the highest chromium tolerant bacterium. The selected bacterium was further taken for their growth studies followed by different cultural, morphological and molecular analysis (16S rDNA). The bacterial strain was further studied through SEM (Scanning Electron microscopy) and EDX (Energy Dispersive X-ray) spectroscopy which revealed that TW4 was a gram positive, rod shaped, endospore forming, pleomorphic bacterium with phylogenetic similarities with Isoptericola sp. and genebank accession number SUB1732465 TW4 KX640927.

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Pharmacokinetics, Pharmacodynamic Efficacy Prediction Indexes and Monte Carlo Simulations of Enrofloxacin Hydrochloride Against Bacterial Strains That Induce Common Clinical Diseases in Broiler Chickens

Frontiers in Veterinary Science ◽

10.3389/fvets.2020.606872 ◽

2021 ◽

Vol 7 ◽

Author(s):

Karina P. D. Bonassa ◽

Miwa Y. Miragliotta ◽

Rosineide C. Simas ◽

Marcos N. Eberlin ◽

Arturo Anadón ◽

...

Keyword(s):

Monte Carlo ◽

Plasma Concentration ◽

Monte Carlo Simulations ◽

Broiler Chickens ◽

Inhibitory Concentration ◽

Plasma Concentrations ◽

Bacterial Strains ◽

E Coli ◽

Concentration Time ◽

Efficacy Prediction

Pharmacokinetic parameters and efficacy prediction indexes (Cmax/MIC90 and AUC0−24/MIC90) of an enrofloxacin hydrochloride (ENR-HCl) veterinary product soluble in water were determined in healthy broiler chickens of both sexes after a single oral dose of ENR-HCl (equivalent to 10 mg ENR base/kg bw). Monte Carlo simulations targeting Cmax/MIC90 = 10 and AUC0−24/MIC90 =125 were also performed based on a set of MIC (minimum inhibitory concentration) values of bacterial strains that induce common clinical diseases in broiler chickens and that showed to be susceptible to ENR-HCl. Plasma concentrations of ENR and its main metabolite ciprofloxacin (CIP) were determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Plasma concentration-time curves were found to fit a non-compartmental open model. The ratio of the area under the plasma concentration-time curve (AUC) of CIP/ENR was 4.91%. Maximum plasma concentrations of 1.35 ± 0.15 μg/mL for ENR-HCl and 0.09 ± 0.01 μg/mL for CIP were reached at 4.00 ± 0.00 h and 3.44 ± 1.01 h, respectively. Areas under the plasma vs. time concentration curve in 24 h (AUC0−24) were 18.91 ± 1.91 h × μg/mL and 1.19 ± 0.12 h × μg/mL for ENR-HCl and CIP, respectively. Using a microbroth dilution method, the minimum inhibitory concentration (MIC90) values were determined for ENR-HCl for 10 bacterial strains (Mycoplasma gallisepticum, Mycoplasma synoviae, Avibacterium paragallinarum, Clostridium perfringens, Escherichia coli, Pseudomonas aeruginosa, Salmonella ser. Enteritidis, Salmonella ser. Gallinarum, Salmonella ser. Pullorum, and Salmonella ser. Typhimurium), which are the most common causes of infectious clinical diseases in broiler chickens. In summary, the PK/PD ratios and Monte Carlo simulation were carried out for ENR-HCl in poultry, which due to its solubility was administered in drinking water. The PK/PD efficacy prediction indexes and Monte Carlo simulations indicated that the ENR-HCl oral dose used in this study is useful for bacterial infections in treating C. perfringens (Gram-positive), E. coli and S. ser. Enteritidis (Gram-negative) and M. gallisepticum bacteria responsible for systemic infections in poultry, predicting a success rate of 100% when MIC ≤ 0.06 μg/mL for E. coli and S. ser. Enteritidis and MIC ≤ 0.1 μg/mL for M. gallisepticum. For C. perfringens, the success rate was 98.26% for MIC ≤ 0.12. However, clinical trials are needed to confirm this recommendation.

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Atmospheric Pressure Microplasma for Antibacterial Silver Nanoparticle/Chitosan Nanocomposites With Tailored Properties

10.26434/chemrxiv.9849806.v1 ◽

2019 ◽

Author(s):

Daye Sun ◽

Jonathan Turner ◽

Nan Jiang ◽

Songsong Zhu ◽

Li Zhang ◽

...

Keyword(s):

Silver Nanoparticle ◽

Atmospheric Pressure ◽

Room Temperature ◽

Polymer Network ◽

Antibacterial Properties ◽

Bacterial Strains ◽

E Coli ◽

Modified Chitosan ◽

First Time

Room temperature atmospheric pressure microplasma (APM) was deployed for the first time for the in situ synthesis of anti-bacterial silver nanoparticle / chitosan (AgNP/CS) nanocomposites. The plasma induced liquid chemistry plays a role in the in situ formation of AgNP, the size distribution of which depends on the silver salt precursor concentration. The microplasma process has also simultaneously tailored the physical properties of the composites, rendering more crosslinked chitosan polymer network with shorter molecular chains. The formation of AgNP within the in situ modified chitosan has led to nanocomposites with overall improved mechanical properties and better stability in simulated body fluid. Our plasma synthesized AgNP/CS nanocomposites also demonstrate effective antibacterial properties against E. Coli and S. Aureus bacterial strains, showing their promise in potential antimicrobial applications.

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In VitroAntimicrobial and Modulatory Activity of the Natural Products Silymarin and Silibinin

BioMed Research International ◽

10.1155/2015/292797 ◽

2015 ◽

Vol 2015 ◽

pp. 1-7 ◽

Cited By ~ 14

Author(s):

Dayanne Rakelly de Oliveira ◽

Saulo Relison Tintino ◽

Maria Flaviana Bezerra Morais Braga ◽

Aline Augusti Boligon ◽

Margareth Linde Athayde ◽

...

Keyword(s):

Antifungal Activity ◽

Inhibitory Concentration ◽

Major Constituent ◽

Significant Activity ◽

Antibacterial And Antifungal Activity ◽

Bacterial Strains ◽

Silybum Marianum ◽

Microbial Strains ◽

Antibacterial And Antifungal ◽

Mic Minimum Inhibitory Concentration

Silymarin is a standardized extract from the dried seeds of the milk thistle (Silybum marianumL. Gaertn.) clinically used as an antihepatotoxic agent. The aim of this study was to investigate the antibacterial and antifungal activity of silymarin and its major constituent (silibinin) against different microbial strains and their modulatory effect on drugs utilized in clinical practice. Silymarin demonstrated antimicrobial activity of little significance against the bacterial strains tested, with MIC (minimum inhibitory concentration) values of 512 µg/mL. Meanwhile, silibinin showed significant activity againstEscherichia coliwith a MIC of 64 µg/mL. The results for the antifungal activity of silymarin and silibinin demonstrated a MIC of 1024 µg/mL for all strains. Silymarin and silibinin appear to have promising potential, showing synergistic properties when combined with antibacterial drugs, which should prompt further studies along this line.

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Evaluation of Biological Activities of Chemically Synthesized Cobalt Oxide Nanoparticles in Concentration and Time Dependent Manner

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2020.13.6.9 ◽

2020 ◽

Vol 13 (6) ◽

pp. 5243-5249

Author(s):

Vijayta Gupta ◽

Vinay Kant ◽

Meena Sharma

Keyword(s):

Free Radicals ◽

Cobalt Oxide ◽

Biological Activities ◽

Antibacterial Properties ◽

Co3o4 Nanoparticles ◽

Dependent Manner ◽

Bacterial Strains ◽

Concentration Dependent Manner ◽

E Coli

The promising results of metal oxides nanoparticles in different areas including the biological system lead us to investigate the antioxidant and antimicrobial actions of chemically synthesized cobalt oxide (Co3O4) nanoparticles. The different concentrations of synthesized Co3O4 nanoparticles were prepared and evaluated for different parameters at different time intervals i.e. on day 1, 30 and 60 after preparations. Co3O4 nanoparticles synthesized in this study were of 52.2 nm average size with a polydispersity index of 0.465. We observed that Co3O4 nanoparticles scavenge different in vitro free radicals (DPPH, ABTS, superoxide anion and hydrogen peroxide radicals) in concentration dependent manner. The percentage of inhibitions of free radicals by Co3O4 nanoparticles was markedly more on day 1 as compared to day 30 and 60. The IC50 values of Co3O4 nanoparticles for these free radicals were also on day 1 as compared to day 30 and 60. The Co3O4 nanoparticles showed the antibacterial actions against both the bacterial strains i.e. S. aureus and E. coli. The MIC and MBC values revealed that action of Co3O4 nanoparticles was more against E. coli than S. aureus. The MIC and MBC values were lower on day 1 as compared to day 30 and 60 with respective to specific bacteria. In conclusions, the Co3O4 nanoparticles synthesized in this study showed potent antioxidant and antibacterial properties due to which it may serve as promising candidate for the combat the biological problems humans, animals and plants associated with reactive oxygen species and bacteria.

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Gram-scale synthesis of splat-shaped Ag–TiO2 nanocomposites for enhanced antimicrobial properties

Beilstein Journal of Nanotechnology ◽

10.3762/bjnano.11.96 ◽

2020 ◽

Vol 11 ◽

pp. 1119-1125

Author(s):

Mohammad Jaber ◽

Asim Mushtaq ◽

Kebiao Zhang ◽

Jindan Wu ◽

Dandan Luo ◽

...

Keyword(s):

Antibacterial Activity ◽

Antibacterial Properties ◽

Antimicrobial Properties ◽

Inhibition Zone ◽

Multidrug Resistant ◽

Bacterial Strains ◽

Gram Negative ◽

E Coli ◽

Contagious Diseases ◽

Good Biocompatibility

The control over contagious diseases caused by pathogenic organisms has become a serious health issue. The extensive usage of antibiotics has led to the development of multidrug-resistant bacterial strains. In this regard, metal-oxide-based antibacterial nanomaterials have received potential research interest due to the efficient prevention of microorganism growth. In this study, splat-shaped Ag–TiO2 nanocomposites (NCs) were synthesized on the gram scale and the enhanced antibacterial properties of TiO2 in the presence of silver were examined. The formation of Ag–TiO2 NCs was analyzed through various characterization techniques. The cell viability experimental results demonstrated that the Ag–TiO2 NCs have good biocompatibility. The antibacterial activity of the prepared Ag–TiO2 NCs was tested against the Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) bacterial strains. The Ag–TiO2 NCs exhibited promising and superior antibacterial properties compared to TiO2 nanospheres as confirmed by the bacterial growth and inhibition zone. The improvement in the antibacterial activity was attributed to the synergistic effect of the hybrid nature of TiO2 nanoparticles in the presence of Ag.

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