scholarly journals Development and Characterization of Active Gelatin Films Loaded with Rapeseed Meal Extracts

Materials ◽  
2021 ◽  
Vol 14 (11) ◽  
pp. 2869
Author(s):  
Alicja Tymczewska ◽  
Bliss Ursula Furtado ◽  
Jacek Nowaczyk ◽  
Katarzyna Hrynkiewicz ◽  
Aleksandra Szydłowska-Czerniak
Keyword(s):  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Rapeseed Meal ◽  
Active Packaging ◽  
Bacterial Strains ◽  
Casting Technique ◽  
E Coli ◽  
Industry Waste ◽  
Gelatin Films ◽  
Radical Scavenging Properties

The use of industrial waste as a material for the development of natural innovative and active packaging is economically and environmentally appealing. The aim of this study was to develop and characterize active gelatin films incorporating rapeseed oil industry waste. Water (RM-WE) and methanolic (RM-MWE) extracts of rapeseed meal (RM) were used as active agents in film formulations. The active films were produced by a casting technique. The physicochemical, mechanical, optical, morphological, radical scavenging, and antibacterial properties of the films were analyzed. The addition of RM-WE and RM-MWE in the concentrations range between 4 and 12% promoted an increase of Young’s modulus (YM) and radical scavenging properties of films investigated by the direct QUick, Easy, New, CHEap and Reproducible procedure using 2,2-diphenyl-1-picrylhydrazyl (QUENCHERDPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6- sulfonic acid) (QUENCHERABTS) radicals. The antibacterial properties of films were examined against five bacterial strains: E. coli, S. enterica, M. luteus, L. monocytogenes, and S. aureus. Additionally, color and opacity of the control and fortified films differed significantly. The gelatin films with RM extracts are resistant to the microbial spoilage and could be used to produce active packaging for food that is vulnerable to rancidity effects.

scholarly journals Decoding Antibacterial and Antibiofilm Properties of Cinnamon and Cardamom Essential Oils: A Combined Molecular Docking and Experimental Study

2021 ◽  
Author(s):  
Elahe Pourkhosravani ◽  
fatemeh dehghan nayeri ◽  
Mitra Mohammadi Bazargani
Keyword(s):  
Cell Attachment ◽  
Antibacterial Properties ◽  
Binding Mode ◽  
Dilution Method ◽  
Antibiofilm Activity ◽  
Bacterial Strains ◽  
Biofilm Growth ◽  
Computational Docking ◽  
E Coli ◽  
Industrial Strains

Abstract This study sets out to compare the antibacterial and antibiofilm profiles of Ci/Ca EOs alone and in combination together against infectious bacterial strains. MIC assay was carried out to survey the effectiveness of prepared EOs by two-fold serial dilution method and MTT evaluation. Synergic antibacterial properties of EOs against target strains were studied by using checkerboard titration method. Biofilm growth and development were evaluated using CV and XTT reduction assays. Antibacterial activity was observed for EOs against both bacterial strains with stronger activity for CiEO against both bacteria. The synergistic antibacterial effect was observed only against B. subtilis. Based on the FIC index, combinations could not inhibit the growth of E. coli. The pure EOs and their combination inhibited cell attachment for both studied bacteria with stronger effect on E. coli. CV and XTT reduction assays results showed that Ci EO and its combination with CaEO had the highest antibiofilm activity at lowest MIC value 0.08% and 0.04/0.02% against biofilm formed by E. coli and B. subtilis respectively, indicating a high antibiofilm potential. Computational docking analyses also postulated that the active constituents of evaluated EOs have the potential to interact with different bacterial targets, suggested binding mode of action of EOs metabolites. By and large, synergistic anti-biofilm properties of EOs may provide further options for developing novel formula to inhibit a variety of infectious clinical and industrial strains without (or less) toxicity effects on human body.

scholarly journals Phytochemical Study and Antibacterial and Antibiotic Modulation Activity of Punica granatum (Pomegranate) Leaves

Scientifica ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Amine Trabelsi ◽  
Mohamed Amine El Kaibi ◽  
Aïmen Abbassi ◽  
Amira Horchani ◽  
Leila Chekir-Ghedira ◽  
...  
Keyword(s):  
Antibacterial Properties ◽  
Punica Granatum ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Phytochemical Study ◽  
E Coli ◽  
Microdilution Method ◽  
Resistant Strains

This study aimed to determine phytochemical contents, antibacterial properties, and antibiotic modulating potential of Punica granatum leaf extracts: hexane, chloroform, ethyl acetate, ethanol, and aqueous extracts as well as an extract enriched with total oligomer flavonoids (TOFs). The TOF extract contained the highest value of phenols and flavonoids. Rutin, luteolin, gallic acid, and ellagic acid were determined by HPLC analysis of this extract. The antibacterial activity was assayed by the disc diffusion method and microdilution method against Staphylococcus aureus and Escherichia coli standard ATCC strains and clinical isolates resistant strains. The TOF extract was the most active against all tested strains. The checkerboard method was used for the determination of synergy between two antibiotics (amoxicillin and cefotaxime) and P. granatum leaf extracts. The best synergistic interaction was found with TOF extract combined with amoxicillin for penicillin-resistant E. coli and penicillin-resistant S. aureus. These results can be assigned to tannins, flavonoids, and phenolic acids found in P. granatum leaf extracts. Pomegranate leaf extracts or active compounds isolated from these extracts could be used to fight the emergence and spread of resistant bacterial strains.

scholarly journals Chitosan & Conductive PANI/Chitosan Composite Nanofibers - Evaluation of Antibacterial Properties

2019 ◽  
Vol 4 (1) ◽  
pp. 6-20 ◽  
Author(s):  
Panagiota Moutsatsou ◽  
Karen Coopman ◽  
Stella Georgiadou
Keyword(s):  
Chronic Wounds ◽  
Antibacterial Properties ◽  
Wound Dressings ◽  
Bacterial Strains ◽  
Healthcare Applications ◽  
E Coli ◽  
Jet Stability ◽  
Electrospun Membranes ◽  
New Treatments ◽  
Chitosan Composite

Background: Within the healthcare industry, including the care of chronic wounds, the challenge of antimicrobial resistance continues to grow. As such, there is a need to develop new treatments that can reduce the bioburden in wounds. Objective: The present study is focused on the development of polyaniline (PANI) / chitosan (CH) nanofibrous electrospun membranes and evaluates their antibacterial properties. Methods: To this end, experimental design was used to determine the electrospinning windows of both pure chitosan and PANI/CH blends of different ratios (1:3, 3:5, 1:1). The effect of key environmental and process parameters (relative humidity and applied voltage) was determined, as well as the effect of the PANI/CH ratio in the blend and the molecular interactions between PANI and chitosan that led to jet stability. Results: The nanofibrous mats were evaluated regarding their morphology and antibacterial effect against model gram positive and gram negative bacterial strains, namely B. subtilis and E. coli. High PANI content mats show increased bactericidal activity against both bacterial strains. Conclusion: The blend fibre membranes combine the materials’ respective properties, namely electrical conductivity, biocompatibility and antibacterial activity. This study suggests that electrospun PANI/CH membranes are promising candidates for healthcare applications, such as wound dressings.

scholarly journals Antioxidant and Radical Scavenging of Piliostigma reticulatum using FRAP and DPPH

2021 ◽  
Keyword(s):  
Methanol Extract ◽  
Antibacterial Effect ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Salmonella Typhi ◽  
Phytochemical Content ◽  
E Coli ◽  
Radical Scavenging Ability ◽  
Radical Scavenging Properties ◽  
Piliostigma Reticulatum

The antibacterial activity, phytochemical constituents and the radical scavenging ability using FRAP and DPPH of Piliostigma reticulatum were investigated in this study. Of all the solvent extracts used to assay the antibacterial effect, ethanol extract had the highest activity, followed by the aqueous extract with very weak activity against Pseudomonsa aeruginosa, Salmonella typhi and E. coli, methanol extract had no visible effect on the test organisms while ethyl acetate was active against only E. coli. The presence of phytochemicals such as alkaloids, glycosides, steroids, anthraquinones, phenol, tanin and saponin was observed. Antinutrients such as Tanin, Phenol, Phylate, Oxalate, Saponin and Flavonoids in copious amount. Ethanol extract of P. reticulatum was found to have the highest antioxidant ability, followed by the methanol extract. Ehtyl acetate extract had minimal radical scavenging ability. The antibacterial effect, phytochemical content and the radical scavenging properties of the plant makes it a good candidate in the development of antimicrobial therapeutics.

scholarly journals Antibacterial effect of TiO2 modified with poly-amidoamine dendrimer – G3 on S. aureus and E. coli in aqueous solutions

2022 ◽  
Author(s):  
Soudabeh Alizadeh Matboo ◽  
Shahram Nazari ◽  
Ali Niapour ◽  
Mehdi Vosoughi Niri ◽  
Esrafil Asgari ◽  
...  
Keyword(s):  
Inhibitory Concentration ◽  
Antibacterial Properties ◽  
Plate Count ◽  
Bacterial Strains ◽  
Solution Ph ◽  
E Coli ◽  
Bacterial Removal ◽  
Mic Minimum Inhibitory Concentration ◽  
Paper Disc ◽  
Surface Plate

Abstract This study investigated the bacterial removal using TiO2 nanoparticles (NPs) modified with poly-amidoamine dendrimer macromolecule (PAMAM, G3). The PAMAM G3/TiO2 (nanohybrid) was used to specify antibacterial properties via broth microdilution (MBC-Minimum Bactericidal Concentration and MIC-Minimum Inhibitory Concentration- determination), paper disc diffusion, and surface plate count methods. The nanohybrid was characterized via the different techniques. The effects of different factors including initial bacteria count, run time, solution pH, and the nanohybrid concentration were studied. The nanohybrid cytotoxicity was studied on AGS and MKN45 cells line by MTT assay. It was revealed that the nanohybrid was effective in intercepting both bacterial strains growth. The MIC value for S. aureus and E. coli were determined to be 4 and 2 μg/mL, respectively. The MBC value for both strains were calculated to be 32 μg/mL. The results showed removal efficiency of 100% for S. aureus and E. coli bacteria in optimum situation. The decrease in cell viability in the dosage of 32 μg/mL after 72 h treatment for AGS and MKN45 cells line were shown to be 6.2 and 4.6%, respectively. The nanohybrid was able to decrease the S. aureus and E. coli count in solution, which meets the drinking water criterions aligned with WHO guidelines.

scholarly journals Elemental Composition, Antioxidant and Antibacterial Properties of Some Wild Edible Mushrooms from Romania

Agronomy ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1972
Author(s):  
Melinda Fogarasi ◽  
Zorița Maria Diaconeasa ◽  
Carmen Rodica Pop ◽  
Szabolcs Fogarasi ◽  
Cristina Anamaria Semeniuc ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Colorimetric Method ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Natural Antioxidants ◽  
Edible Mushrooms ◽  
Wild Edible Mushrooms ◽  
Mushroom Species ◽  
E Coli ◽  
Daily Diet

Five selected wild edible mushrooms from Romania (Agaricus bisporus, Pleurotus ostreatus, Cantharellus cibarius, Boletus edulis, and Lactarius piperatus) were investigated for their antioxidant potential using an ABTS spectrophotometric assay. Among the selected mushrooms, B. edulis displayed the highest radical scavenging activity and the greatest phenolic content, measured by the Folin–Ciocalteu reagent method. The total flavonoids were quantified using the aluminum chloride colorimetric method, with the extract of B. edulis being the richest. L. piperatus and B. edulis mushrooms exhibited the strongest antibacterial activity against S. aureus and E. coli. The content of trace elements was determined using an atomic absorption spectrometer, and it was found that K and Mg were the main metals present in all the selected mushroom species. The obtained results suggest that the studied wild edible mushrooms are natural functional matrices, and may have potential to be used as natural antioxidants if they are introduced into the daily diet.

scholarly journals Determination of Phenolic Compounds in Various Propolis Samples Collected from an African and an Asian Region and Their Impact on Antioxidant and Antibacterial Activities

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4589
Author(s):  
Soumaya Touzani ◽  
Hamada Imtara ◽  
Shankar Katekhaye ◽  
Hamza Mechchate ◽  
Hayat Ouassou ◽  
...  
Keyword(s):  
Cinnamic Acid ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Total Phenolic ◽  
Bacterial Strains ◽  
Photodiode Array Detection

The biological activities of propolis samples are the result of many bioactive compounds present in the propolis. The aim of the present study was to determine the various chemical compounds of some selected propolis samples collected from Palestine and Morocco by the High-Performance Liquid Chromatography–Photodiode Array Detection (HPLC-PDA) method, as well as the antioxidant and antibacterial activities of this bee product. The chemical analysis of propolis samples by HPLC-PDA shows the cinnamic acid content in the Palestinian sample is higher compared to that in Moroccan propolis. The results of antioxidant activity demonstrated an important free radical scavenging activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH); 2,2′-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and reducing power assays) with EC50 values ranging between 0.02 ± 0.001 and 0.14 ± 0.01 mg/mL. Additionally, all tested propolis samples possessed a moderate antibacterial activity against bacterial strains. Notably, Minimum Inhibitory Concentrations (MICs) values ranged from 0.31 to 2.50 mg/mL for Gram-negative bacterial strains and from 0.09 to 0.125 mg/mL for Gram-positive bacterial strains. The S2 sample from Morocco and the S4 sample from Palestine had the highest content of polyphenol level. Thus, the strong antioxidant and antibacterial properties were apparently due to the high total phenolic and flavone/flavonol contents in the samples. As a conclusion, the activities of propolis samples collected from both countries are similar, while the cinnamic acid in the Palestinian samples was more than that of the Moroccan samples.

scholarly journals Atmospheric Pressure Microplasma for Antibacterial Silver Nanoparticle/Chitosan Nanocomposites With Tailored Properties

2019 ◽  
Author(s):  
Daye Sun ◽  
Jonathan Turner ◽  
Nan Jiang ◽  
Songsong Zhu ◽  
Li Zhang ◽  
...  
Keyword(s):  
Silver Nanoparticle ◽  
Atmospheric Pressure ◽  
Room Temperature ◽  
Polymer Network ◽  
Antibacterial Properties ◽  
Bacterial Strains ◽  
E Coli ◽  
Modified Chitosan ◽  
First Time

<p>Room temperature atmospheric pressure microplasma (APM) was deployed for the first time for the in situ synthesis of anti-bacterial silver nanoparticle / chitosan (AgNP/CS) nanocomposites. The plasma induced liquid chemistry plays a role in the in situ formation of AgNP, the size distribution of which depends on the silver salt precursor concentration. The microplasma process has also simultaneously tailored the physical properties of the composites, rendering more crosslinked chitosan polymer network with shorter molecular chains. The formation of AgNP within the <i>in situ</i> modified chitosan has led to nanocomposites with overall improved mechanical properties and better stability in simulated body fluid. Our plasma synthesized AgNP/CS nanocomposites also demonstrate effective antibacterial properties against <i>E. Coli</i> and <i>S. Aureus</i> bacterial strains, showing their promise in potential antimicrobial applications.</p>

Evaluation of Biological Activities of Chemically Synthesized Cobalt Oxide Nanoparticles in Concentration and Time Dependent Manner

2020 ◽  
Vol 13 (6) ◽  
pp. 5243-5249
Author(s):  
Vijayta Gupta ◽  
Vinay Kant ◽  
Meena Sharma
Keyword(s):  
Free Radicals ◽  
Cobalt Oxide ◽  
Biological Activities ◽  
Antibacterial Properties ◽  
Co3o4 Nanoparticles ◽  
Dependent Manner ◽  
Bacterial Strains ◽  
Concentration Dependent Manner ◽  
E Coli

The promising results of metal oxides nanoparticles in different areas including the biological system lead us to investigate the antioxidant and antimicrobial actions of chemically synthesized cobalt oxide (Co3O4) nanoparticles. The different concentrations of synthesized Co3O4 nanoparticles were prepared and evaluated for different parameters at different time intervals i.e.  on day 1, 30 and 60 after preparations.  Co3O4 nanoparticles synthesized in this study were of 52.2 nm average size with a polydispersity index of 0.465. We observed that Co3O4 nanoparticles scavenge different in vitro free radicals (DPPH, ABTS, superoxide anion and hydrogen peroxide radicals) in concentration dependent manner. The percentage of inhibitions of free radicals by Co3O4 nanoparticles was markedly more on day 1 as compared to day 30 and 60. The IC50 values of Co3O4 nanoparticles for these free radicals were also on day 1 as compared to day 30 and 60. The Co3O4 nanoparticles showed the antibacterial actions against both the bacterial strains i.e. S. aureus and E. coli. The MIC and MBC values revealed that action of Co3O4 nanoparticles was more against E. coli than S. aureus. The MIC and MBC values were lower on day 1 as compared to day 30 and 60 with respective to specific bacteria. In conclusions, the Co3O4 nanoparticles synthesized in this study showed potent antioxidant and antibacterial properties due to which it may serve as promising candidate for the combat the biological problems humans, animals and plants associated with reactive oxygen species and bacteria.

scholarly journals Gram-scale synthesis of splat-shaped Ag–TiO2 nanocomposites for enhanced antimicrobial properties

2020 ◽  
Vol 11 ◽  
pp. 1119-1125
Author(s):  
Mohammad Jaber ◽  
Asim Mushtaq ◽  
Kebiao Zhang ◽  
Jindan Wu ◽  
Dandan Luo ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Multidrug Resistant ◽  
Bacterial Strains ◽  
Gram Negative ◽  
E Coli ◽  
Contagious Diseases ◽  
Good Biocompatibility

The control over contagious diseases caused by pathogenic organisms has become a serious health issue. The extensive usage of antibiotics has led to the development of multidrug-resistant bacterial strains. In this regard, metal-oxide-based antibacterial nanomaterials have received potential research interest due to the efficient prevention of microorganism growth. In this study, splat-shaped Ag–TiO2 nanocomposites (NCs) were synthesized on the gram scale and the enhanced antibacterial properties of TiO2 in the presence of silver were examined. The formation of Ag–TiO2 NCs was analyzed through various characterization techniques. The cell viability experimental results demonstrated that the Ag–TiO2 NCs have good biocompatibility. The antibacterial activity of the prepared Ag–TiO2 NCs was tested against the Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) bacterial strains. The Ag–TiO2 NCs exhibited promising and superior antibacterial properties compared to TiO2 nanospheres as confirmed by the bacterial growth and inhibition zone. The improvement in the antibacterial activity was attributed to the synergistic effect of the hybrid nature of TiO2 nanoparticles in the presence of Ag.

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