scholarly journals Biological Activities of Tetrodotoxin-ProducingEnterococcus faeciumAD1 Isolated from Puffer Fishes

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Tu Hoang Khue Nguyen ◽  
Huu Ngoc Nguyen ◽  
Dat Van Nghe ◽  
Kim Hoang Nguyen
Keyword(s):  
Enterococcus Faecium ◽  
High Performance ◽  
Biological Activities ◽  
Antimicrobial Activities ◽  
16S Rrna Analysis ◽  
Sulforhodamine B ◽  
Biological Compounds ◽  
Pangasius Bocourti ◽  
Promising Source ◽  
Mrs Broth

Puffer fishes were collected from the central sea in Vietnam from spring to summer season. The eggs were incubated in MRS broth that was used to test the toxicity in mice and isolate the lactic acid bacteria community that could produce tetrodotoxin (TTX). Thin layer chromatography (TLC) and high performance lipid chromatography (HPLC) were used to detect and quantify TTX. As a result,Enterococcus faeciumAD1 which was identified by biochemical test and 16S rRNA analysis could produce TTX 0.3 mg/mL when cultured in MRS broth. The bacterium was optimized for TTX production and gave 0.18 mg/mL, 0.07 mg/mL, and 0.15 mg/mL in media prepared from the meat-washing water of freshwater fishes (Pangasius bocourti,Oreochromissp.) and sea fish (Auxis thazard), respectively, that are also hopeful to answer some poisoning cases related to eating fishes.Enterococcus faeciumalso showed the wide antimicrobial activities on yeast, Gram-negative and -positive bacteria. Extracted exopolysaccharide (EPS) that reacted with 2,2-diphenyl-1-picrylhydrazyl to give IC50at 5 mg/mL equaled 11 mg/mL ascorbic acid which could show effects on Hela-6 and Hep G2 using sulforhodamine B test.Enterococcus faeciumcan be claimed as a promising source in tetrodotoxin and biological compounds.

scholarly journals Red Propolis as a Source of Antimicrobial Phytochemicals: Extraction Using High-Performance Alternative Solvents

2021 ◽  
Vol 12 ◽  
Author(s):  
Cíntia M. dos Santos ◽  
Leonardo M. de Souza Mesquita ◽  
Anna Rafaela C. Braga ◽  
Veridiana V. de Rosso
Keyword(s):  
High Performance ◽  
Salmonella Enteritidis ◽  
New Technologies ◽  
Biological Activities ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Total Flavonoids ◽  
Future Studies ◽  
Operational Conditions ◽  
Total Antioxidant

Propolis is a resinous material rich in flavonoids and involved in several biological activities such as antimicrobial, fungicide, and antiparasitic functions. Conventionally, ethanolic solutions are used to obtain propolis phytochemicals, which restrict their use in some cultures. Given this, we developed an alcohol-free high-performance extractive approach to recover antibacterial and antioxidants phytochemicals from red propolis. Thus, aqueous-solutions of ionic liquids (IL) and eutectic solvents were used and then tested for their total flavonoids, antioxidant, and antimicrobial activities. The surface-responsive technique was applied regarding some variables, namely, the time of extraction, the number of extractions, and cavitation power (W), to optimize the process (in terms of higher yields of flavonoids and better antioxidant activity). After that, four extractions with the same biomass (repetitions) using 1-hexyl-3-methylimidazolium chloride [C6mim]Cl, under the operational conditions fixed at 3.3 min and 300 W, were able to recover 394.39 ± 36.30 mg RuE. g−1 of total flavonoids, with total antioxidant capacity evaluated up to 7595.77 ± 5.48 μmol TE. g−1dried biomass, besides inhibiting the growth of Staphylococcus aureus and Salmonella enteritidis bacteria (inhibition halo of 23.0 ± 1.0 and 15.7 ± 2.1, respectively). Aiming at the development of new technologies, the antimicrobial effect also presented by [C6mim]Cl may be appealing, and future studies are required to understand possible synergistic actions with propolis phytochemicals. Thereby, we successfully applied a completely alcohol-free method to obtain antimicrobials phytochemicals and highly antioxidants from red propolis, representing an optimized process to replace the conventional extracts produced until now.

scholarly journals Metabolomic Profiling and Antioxidant, Anticancer and Antimicrobial Activities of Hyphaene thebaica

Processes ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 266
Author(s):  
Ghada A. Taha ◽  
Ibrahim B. Abdel-Farid ◽  
Hassan A. Elgebaly ◽  
Usama A. Mahalel ◽  
Mohamed G. Sheded ◽  
...  
Keyword(s):  
High Performance ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Methanolic Extracts ◽  
Carcinoma Cell Lines ◽  
Metabolomic Profiling ◽  
Total Antioxidant ◽  
Positive Correlations

This study investigated the metabolic content and biological activities of Hyphaene thebaica leaves, male parts and fruits methanolic extracts. The phytochemical constituents were determined, and multivariate data analysis discriminated the evaluated three parts into three groups according to the content of phenolics, flavonoids, flavonols, saponins, anthocyanins and tannins. High-performance liquid chromatography (HPLC) profiling of polyphenols revealed the dominance of catechins, rutin and apigenin-7-glucosides in leaves, protocatechuin, p-hydroxybenzoic, syringic, vanillic, rosmarinic, p-coumaric acids and chrysin in male parts, and chlorogenic acid in fruits. Leaves and male parts showed stronger free radical scavenging activity than the fruits. Positive correlations between total antioxidant capacity and carbohydrates, phenolics and flavonols were observed. The three extracts exhibited potent anti-cancer activity against liver and lung carcinoma cell lines. All extracts exhibited antibacterial activity, while only fruits showed antifungal efficacy. In conclusion, H. thebaica leaves, male parts and fruits contain a variety of phytochemicals with antioxidant, anticancer and antimicrobial activities.

Phytochemical and Biological Activities of five Turanecio Hamzaoglu (Asteraceae) Species from Turkey

2017 ◽  
Vol 13 (1) ◽  
pp. 49-55
Author(s):  
Sevil Albayrak ◽  
Ahmet Aksoy ◽  
Abit Yasar ◽  
Lutfiye Yurtseven ◽  
Umit Budak
Keyword(s):  
Therapeutic Potential ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Natural Antioxidants ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
In Vitro Assays ◽  
Methanolic Extracts ◽  
Promising Source

Objectives: In vitro biological activities of methanolic extracts of five Turanecio species have been studied. Materials and Methods: The phenolic compositions of the extracts were evaluated by the Folin- Ciocalteu assay and by HPLC analysis. Antioxidant activities were determined with two in vitro assays namely, DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging assay and phosphomolybdenum assay. The antimicrobial activities of the extracts were evaluated against 13 microorganisms. Results: T. hypochionaeus var. hypochionaeus was showed the highest DPPH inhibition with 88.84% at 100 μg·mL-1. All of the extracts were exerted high total antioxidant activities 128.00- 243.13 mg AAE g-1) and seem to be a promising source of natural antioxidants. The phenolic contents in the extracts varied from 26.17 to 60.99 mg·g-1 as gallic acid equivalent. Chlorogenic acid, caffeic acid and p- coumaric acid were the predominant constituents. The methanolic extracts revealed promising antibacterial activities against most bacteria. No activity was recorded against yeasts tested. Conclusion: The polyphenolic constituents appear to be responsible, at least in part, for the extract’s activities. The present study confirms that tested Turanecio species contains significant source of phenolics have antioxidant and antimicrobial activities and may have therapeutic potential.

scholarly journals Investigation of the Chemical Composition and Antioxidant and Antimicrobial Activities of Lobularia maritima: Potent Therapeutic Applications

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Soumaya Kouidhi ◽  
Oumaima Zidi ◽  
Soukaina Abdelwahed ◽  
Yasmine Souissi ◽  
Najla Trabelsi ◽  
...  
Keyword(s):  
Biological Activities ◽  
Antioxidant Properties ◽  
Extraction Methods ◽  
Antimicrobial Activities ◽  
Biological Properties ◽  
Aspergillus Ochraceus ◽  
Therapeutic Applications ◽  
Lobularia Maritima ◽  
Promising Source ◽  
Antioxidant And Antimicrobial Activities

Lobularia maritima, commonly known as sweet alyssum, is an annual ornamental halophyte widely spread along the Tunisian seashore. However, little is known about the phytochemical, antioxidant, and antimicrobial activities of Lobularia maritima. The present study aimed to investigate the potential biological properties of different parts (flowers, leaves, roots, and stems) of Tunisian L. maritima using diverse extraction methods. Extracts were then studied for their antioxidant properties, and the highest antioxidant activity was presented in the roots’ fractions. Added to this, flower, leaf, and root fractions showed interesting antimicrobial and antifungal activities against different Gram+ and Gram− bacteria and against Aspergillus ochraceus. Finally, the most active fractions (presenting the highest biological activities) were analyzed using silica gel purification and mass spectrometry coupled to gas chromatography (GC-MS) analysis, and different compounds were identified such as camphor, amide of oleic acids, tributyl acetylcitrate, betulinaldehyde, menthol, 1′-(butyn-3-one-1-yl)-, (1S, 2S, 5R), benzyl benzoate, 7-acetyl-6-ethyl-1,1,4,4-tetramethyltetralin, 2,4-heptadienal, (E,E), and nootkaton-11,12-epoxide. This work represents the first in-depth investigation of the content of bioactive compounds from Lobularia maritima. This species could potentially be a promising source of useful compounds for therapeutic applications.

scholarly journals Antimicrobial activities of secondary metabolites of endophytic fungi isolated from Catharanthus roseus

2017 ◽  
Vol 7 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Mark O. Akpotu ◽  
Peter M. Eze ◽  
Chika C. Abba ◽  
Blessing O. Umeokoli ◽  
Charles U. Nwachukwu ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Endophytic Fungi ◽  
Catharanthus Roseus ◽  
High Performance ◽  
Antimicrobial Activities ◽  
Antibacterial And Antifungal Activities ◽  
Rice Medium ◽  
Antibacterial And Antifungal ◽  
Promising Source

Introduction: Recently, several endophytes have been shown to possess the potentials to synthesize novel bioactive compounds that have found use for drug discovery. We isolated endophytic fungi associated with Catharanthus roseus collected from the river banks of Amassoma in Southern Nigeria, and identified some of their bioactive secondary metabolites. Methods: The fungi were subjected to solid-state fermentation on rice medium and the metabolites were extracted using ethyl acetate. The fungal crude extracts were screened for antimicrobial activity and were also subjected to high-performance liquid chromatography-diode-array detection (HPLC-DAD) analysis for the identification of the bioactive compounds. Results: The fungal extracts showed both antibacterial and antifungal activities with minimum inhibitory concentrations ranging from 0.0625 to 1 mg/mL. The HPLC-DAD analysis of the extracts suggested the presence of citreoisocoumarin, citreoisocoumarinol, questinol, hydroxyemodin, acropyrone, methyl 2-(4-hydroxyphenyl) acetate, nigricinol, and cladosporin. Conclusion: The results of this study suggest that endophytic fungi associated with C. roseus could be a promising source of novel bioactive compounds with pharmaceutical and industrial importance.

ANTIMICROBIAL, ANTIOXIDANT ACTIVITY, AND CHEMICAL COMPOSITION OF ORIGANUM COMPACTUM BENTH FROM TAOUNATE PROVINCE, NORTH MOROCCO

2020 ◽  
pp. 126-131
Author(s):  
AHMED ZEROUAL ◽  
NOUREDDINE ELOUTASSI ◽  
MAHDI CHAOUCH ◽  
ABDELLAH CHAQROUNE
Keyword(s):  
Chemical Composition ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Chemical Compounds ◽  
Antimicrobial Activities ◽  
Local Conditions ◽  
Hexane Extracts ◽  
Origanum Compactum ◽  
Promising Source ◽  
Important Activity

Objective: Origanum compactum is one of the most important medicinal species in terms of ethnobotany in Morocco. It attracts the attention of several research works; however, its chemical composition under local conditions is not well documented. Here, we aimed at determining essential oil chemical composition, antibacterial, and antioxidant activities of organic extracts of O. compactum. Methods: Antimicrobial activities were assessed against four strains (Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Candida albicans). Antioxidant activities were estimated by radical 2,2-diphenyl-1-picrylhydrazyl scavenging activity. Polyphenols and flavonoids were determined. Chemical composition was screened using gas chromatography. Results: Our results showed that yield extracts varied significantly among solvents and ranged from 10.30% (n-hexane) to 31.70% (methanolic). Methanolic extracts had the highest values of yield, polyphenols, and flavonoids, while n-hexane extracts showed the lowest values of yield and polyphenols. Regarding antimicrobial activities, E. coli showed the most important activity. Concerning antioxidant activities, n-hexane extracts had the most important activity. The phytochemical screening revealed 12 compounds. Among them, γ-Terpinene, o-Cymene, and carvacrol were the major compounds (around 73% of total chemical compounds). Conclusion: It could be concluded that the studied plant might be a promising source of chemical compounds responsible for important biological activities.

scholarly journals Innovative Biochemometric Approach to the Metabolite and Biological Profiling of the Balkan Thistle (Cirsium appendiculatum Griseb.), Asteraceae

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2046
Author(s):  
Dimitrina Zheleva-Dimitrova ◽  
Gokhan Zengin ◽  
Gunes Ak ◽  
Kouadio Ibrahime Sinan ◽  
Mohamad Fawzi Mahomoodally ◽  
...  
Keyword(s):  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Biological Activities ◽  
Reducing Power ◽  
Partial Least Square ◽  
Least Square ◽  
Root Extract ◽  
Partial Least Square Analysis ◽  
Inhibitory Potential ◽  
Promising Source

The widespread genus Cirsium Mill. (Asteraceae) is renowned in traditional medicine. In the present study, an innovative biochemometric-assisted metabolite profiling of the flower heads, aerial parts and roots of Cirsium appendiculatum Griseb. (Balkan thistle) in relation to their antioxidant and enzyme inhibitory potential was developed. The workflow combines ultra-high-performance liquid chromatography–high-resolution mass spectrometry (UHPLC–HRMS) with partial least-square analysis to discriminate the herbal extracts and identify the most prominent biological activities. The annotation and dereplication of 61 secondary metabolites were evidenced, including 15 carboxylic (including hydroxybenzoic and hydroxycinnamic) acids and their glycosides, 11 acylquinic acids, 26 flavonoids and 9 fatty acids. All compounds were reported for the first time in the studied species. The root extract revealed the highest cupric and ferric reducing power (618.36 ± 5.17 mg TE/g and 269.89 ± 8.50 mg TE/g, respectively) and antioxidant potential in phosphomolybdenum (3.36 ± 0.15 mmol TE/g) as well as the most prominent enzyme inhibitory potential on α-glucosidase (0.72 ± 0.07 mmol ACAE/g), acetylcholinesterase (4.93 ± 0.25 mg GALAE/g) and butyrylcholinesterase (3.80 ± 0.26 mg GALAE/g). Nevertheless, the flower heads were differentiated by their higher metal chelating activity (32.53 ± 3.51 mg EDTAE/g) and total flavonoid content (46.59 ± 0.89 mgRE/g). The partial least-square discriminant and heat-map analysis highlighted the root extract as the most active and a promising source of bioactive compounds for the therapeutic industry.

scholarly journals Characteristics, Phytochemical Analysis and Biological Activities of Extracts from Tunisian ChetouiOlea europaeaVariety

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Ines Khlif ◽  
Karim Jellali ◽  
Thomas Michel ◽  
Maria Halabalaki ◽  
Alexios Leandros Skaltsounis ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
High Performance ◽  
Biological Activities ◽  
Antioxidant Property ◽  
Antimicrobial Activities ◽  
Phytochemical Analysis ◽  
Polar Extracts ◽  
Layer Chromatography

This study selected 10 extracts from Tunisian chetouiO. europaeavariety for their total phenolics, flavonoids, and phytochemical analyses as well as for their antioxidant and antimicrobial activities determination. Thein vitroantioxidant property was investigated using DPPH, ferric reducing antioxidant capacity (FRAP), oxygen reducing antioxidant capacity (ORAC), andβ-carotene-linoleic acid bleaching assays while antimicrobial activity was evaluated using macrodilutions method. For all organs of chetouiO. europaeavariety, the investigated activities were found to be higher in the polar extracts (ethyl acetate, methanol, and methanol/water). These activities were correlated with the presence of phenolic compounds. Phytochemical analyses revealed that the crude extracts contain triterpenoids, quinones, and flavonoids. High performance liquid chromatography (HPLC) and high performance thin layer chromatography (HPTLC) confirmed the presence of phenolic compounds in the studied extracts.

scholarly journals Influence of Extraction Solvent on the Phenolic Profile and Bioactivity of Two Achillea Species

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1601
Author(s):  
Dominika Kaczorová ◽  
Erna Karalija ◽  
Sabina Dahija ◽  
Renata Bešta-Gajević ◽  
Adisa Parić ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
Phenolic Composition ◽  
Extraction Solvent ◽  
Ethanolic Extracts ◽  
Liquid Chromatography Tandem Mass

The phenolic composition, as well as the antioxidant and antimicrobial activities of two poorly investigated Achillea species, Achillea lingulata Waldst. and the endemic Achillea abrotanoides Vis., were studied. To obtain a more detailed phytochemical profile, four solvents with different polarities were used for the preparation of the plant extracts whose phenolic composition was analyzed using UHPLC-MS/MS (ultra-high performance liquid chromatography-tandem mass spectrometry). The results indicate that both of the investigated Achillea species are very rich in both phenolic acids and flavonoids, but that their profiles differ significantly. Chloroform extracts from both species had the highest yields and were the most chemically versatile. The majority of the examined extracts showed antimicrobial activity, while ethanolic extracts from both species were potent against all tested microorganisms. Furthermore, the antioxidant activity of the extracts was evaluated. It was found that the ethanolic extracts possessed the strongest antioxidant activities, although these extracts did not contain the highest amounts of detected phenolic compounds. In addition, several representatives of phenolic compounds were also assayed for these biological activities. Results suggest that ethanol is a sufficient solvent for the isolation of biologically active compounds from both Achillea species. Moreover, it was shown that the flavonoids naringenin and morin are mainly responsible for these antimicrobial activities, while caffeic, salicylic, chlorogenic, p-coumaric, p-hydroxybenzoic, and rosmarinic acid are responsible for the antioxidant activities of the Achillea extracts.

Synthesis, Antimicrobial Evaluation and Docking Study of Novel Thiosemicarbazone clubbed with 1,2,3-Triazoles

2020 ◽  
Vol 16 ◽  
Author(s):  
Adinath D. Badar ◽  
Shubham M. Sulakhe ◽  
Mahesh B. Muluk ◽  
Naziya N. M. A. Rehman ◽  
Prashant P. Dixit ◽  
...  
Keyword(s):  
Binding Affinity ◽  
Biological Activities ◽  
Dna Gyrase ◽  
Docking Study ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Molecular Docking Study ◽  
Triazole Derivatives ◽  
Antibacterial And Antifungal

Background: Thiosemicarbazone, 1,2,3-triazole and their derivatives received great pharmaceutical importance due to their prominent biological activities. In the present study, the molecular hybrid thiosemicarbazone-1,2,3-triazoles derivatives were synthesized and screened for their antimicrobial activities. Methods: A series of thiosemicarbazone clubbed with 1,2,3-triazole derivatives were synthesized via click chemistry approach in good yields. The structures of synthesized compounds were assigned by their spectral data. The in vitro antimicrobial activity was performed by the agar well diffusion method. A molecular docking study was performed to identify the possible mode of action of synthesized derivatives. Results: The compounds 5d, 5h, 5i and 5k have exhibited excellent antimicrobial activities against both antibacterial and antifungal pathogens. The active thiosemicarbazone-1,2,3-triazole derivatives have shown excellent binding affinity towards DNA gyrase. Conclusion: The molecular hybrid thiosemicarbazone-1,2,3-triazole derivatives were synthesized. The newly synthesized compounds were evaluated for their antimicrobial activities. Few of the thiosemicarbazone-1,2,3-triazoles derivatives have exhibited good antimicrobial activities. They have been shown excellent binding affinity towards DNA gyrase.

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