scholarly journals Influence of Extraction Solvent on the Phenolic Profile and Bioactivity of Two Achillea Species

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1601
Author(s):  
Dominika Kaczorová ◽  
Erna Karalija ◽  
Sabina Dahija ◽  
Renata Bešta-Gajević ◽  
Adisa Parić ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
Phenolic Composition ◽  
Extraction Solvent ◽  
Ethanolic Extracts ◽  
Liquid Chromatography Tandem Mass

The phenolic composition, as well as the antioxidant and antimicrobial activities of two poorly investigated Achillea species, Achillea lingulata Waldst. and the endemic Achillea abrotanoides Vis., were studied. To obtain a more detailed phytochemical profile, four solvents with different polarities were used for the preparation of the plant extracts whose phenolic composition was analyzed using UHPLC-MS/MS (ultra-high performance liquid chromatography-tandem mass spectrometry). The results indicate that both of the investigated Achillea species are very rich in both phenolic acids and flavonoids, but that their profiles differ significantly. Chloroform extracts from both species had the highest yields and were the most chemically versatile. The majority of the examined extracts showed antimicrobial activity, while ethanolic extracts from both species were potent against all tested microorganisms. Furthermore, the antioxidant activity of the extracts was evaluated. It was found that the ethanolic extracts possessed the strongest antioxidant activities, although these extracts did not contain the highest amounts of detected phenolic compounds. In addition, several representatives of phenolic compounds were also assayed for these biological activities. Results suggest that ethanol is a sufficient solvent for the isolation of biologically active compounds from both Achillea species. Moreover, it was shown that the flavonoids naringenin and morin are mainly responsible for these antimicrobial activities, while caffeic, salicylic, chlorogenic, p-coumaric, p-hydroxybenzoic, and rosmarinic acid are responsible for the antioxidant activities of the Achillea extracts.

scholarly journals Phenolic compounds and biological activities of rye (Secale cereale L.) grains

2019 ◽  
Vol 17 (1) ◽  
pp. 988-999 ◽  
Author(s):  
Katarína Kulichová ◽  
Jozef Sokol ◽  
Peter Nemeček ◽  
Mária Maliarová ◽  
Tibor Maliar ◽  
...  
Keyword(s):  
Secale Cereale ◽  
High Performance ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Biologically Active ◽  
Spectrophotometric Methods ◽  
Secale Cereale L ◽  
Inhibitory Activities ◽  
Traditional Bread

AbstractThe rye flour is, together with the wheat flour, the basic ingredient used in traditional bread baking. The rye grain contains many compounds with significant impacts on the consumer. Considering that, various biologically active phytochemicals were determined in extracts from mature grains of 19 rye genotypes (Secale cereale L.). The content of total phenols, flavonoids, phenolic acids and thiols, as well as antioxidant activities and inhibitory activities against trypsin, thrombin, and urokinase were analyzed by spectrophotometric methods. The vanillic acid, vanillin, p-coumaric acid, and t-ferulic acid were analyzed in particular by high performance liquid chromatography (HPLC). The observed differences in the amounts and activities between rye genotypes reflected variations in their genetic background. Rye grain is a remarkable source of specific phytochemicals. Genetic diversity in rye makes it possible to identify individual genotypes that have a unique content and biological activity of compounds deposited in mature grains. One subgroup of rye genotypes had higher values of antioxidant properties and concentrations of polyphenols. Other sub-group had higher proteinase inhibitory activities and contents of polyphenols. The third sub-group contained as though the universal genotypes, i.e. genotypes with average values in nearly all the measured parameters.

Comparative analysis of antioxidant activity and phenolic compounds in the fruits of Aronia spp.

10.5219/1360 ◽  
2020 ◽  
Vol 14 ◽  
pp. 393-401
Author(s):  
Yulia Vinogradova ◽  
Olena Vergun ◽  
Olga Grygorieva ◽  
Eva Ivanišová ◽  
Ján Brindza
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Reducing Power ◽  
Biologically Active ◽  
Polyphenol Compounds ◽  
Dpph Method ◽  
Ethanol Extracts

Chokeberry (Aronia Medik.) is a non-traditional fruit plant known as a rich source of biologically active compounds and inhibits the numerous biological activities. We compared the antioxidant activity and phenolic compounds of fruits between widely cultivated Aronia mitschurinii (AM-TCH, from Tchekhov district; AM-D, from Dmitrov district; AM-OZ, from Orekhovo-Zuevsky district of Moscow region, Russia) and introduced North American Aronia species (Aronia arbutifolia (AA-M), A. melanocarpa (AML-M), A. × prunifolia (AP-M), which have not been planted yet in the arboretum of Main Botanical Garden of the Russian Academy of Sciences (Moscow). Studying samples were collected in their secondary distribution range. Ethanolic extracts were determined for antioxidant capacity (antioxidant activity by DPPH and phosphomolybdenum methods, the total content of polyphenols, flavonoids, phenolic acids) and measured spectrophotometrically. As standards were used Trolox (TE) for antioxidant activities, gallic acid (GAE) for polyphenol content, quercetin (QE) for flavonoid content, caffeic acid (CAE) for phenolic acid content. The antioxidant activity by DPPH method in ethanol extracts of investigated plants was from 6.96 (AM-D) to 8.89 (AM-OZ) mg TE.g-1 DW. Reducing the power of investigated extracts exhibited activity from 151.47 (AM-OZ) to 297.8 (AA-M) mg TE.g-1 DW. The content of polyphenol compounds determined from 25.98 (AM-TCH) to 54.39 (AA-M) mg GAE.g-1 DW, phenolic acids content was from 7.76 (AP-M) to 11.87 (AM-D) mg CAE.g-1 DW and the content of flavonoids detected from 8.12 (AM-OZ) to 16.62 (AM-D) mg QE.-1 DW. Obtained data showed a strong correlation between the content of polyphenol compounds and reducing the power of extracts (r = 0.700), between flavonoids and phenolic acids (r = 0.771) and also between phenolic acids and reducing power (r = 0.753) in Aronia ethanol extracts. Fruits of investigated species of Aronia can be propagated as a source of polyphenol compounds with antioxidant activity and obtained results may use for farther pharmacological study.

scholarly journals Metabolite Profiling of the Indian Food Spice Lichen, Pseudevernia furfuracea Combined With Optimised Extraction Methodology to Obtain Bioactive Phenolic Compounds

2021 ◽  
Vol 12 ◽  
Author(s):  
Rishu Kalra ◽  
Xavier A. Conlan ◽  
Carlos Areche ◽  
Rahul Dilawari ◽  
Mayurika Goel
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Extraction Process ◽  
Epiphytic Lichen ◽  
Hek 293 ◽  
Extraction Solvent ◽  
Pseudevernia Furfuracea

Pseudevernia furfuracea (L.) Zopf (Parmeliaceae) is a well-known epiphytic lichen commonly used in Indian spice mixtures and food preparations such as curries. This study is an attempt to find the best extraction methodology with respect to extractive yield, total polyphenolic content (TPC), total flavonoid content and antioxidant activities of lichen P. furfuracea. Two phenolic compounds, atraric acid and olivetoric acid were isolated and quantified in their respective extracts with the aid of reverse phase high performance liquid chromatography (RP-HPLC). The highest concentration of both the compounds, atraric acid (4.89 mg/g DW) and olivetoric acid (11.46 mg/g DW) were found in 70% methanol extract. A direct correlation was also observed between the concentrations of these compounds with the free radical scavenging potential of the extracts which might contribute towards the antioxidant potential of the extract. Moreover, scanning electron microscopy and HPLC analysis which was used to study the effect of pre-processing on extraction process highlighted the capacity of a mixer grinder technique for improved separation of surface localized metabolites and enrichment of the fraction. An investigation of the chemical profile of the bioactive extract 70% methanol extract using UHPLC-DAD-MS lead to tentative identification of forty nine compounds. This extract was also assessed towards HEK 293 T cell line for cytotoxicity analysis. Concentration range of 0.156 to 100 µg/ml of PF70M extract exhibited no significant cell death as compared to control. Further, the active extract showed protective effect against hydroxyl radical’s destructive effects on DNA when assessed using DNA nicking assay. Based upon this, it can be concluded that optimization of extraction solvent, sample pre-proceesing and extraction techniques can be useful in extraction of specific antioxidant metabolites.

scholarly journals The biological activities of Arabis alpina L. subsp. brevifolia (DC.) Cullen against food pathogens

2018 ◽  
Vol 16 (1) ◽  
pp. 930-936 ◽  
Author(s):  
N. Balpinar
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Food Pathogens ◽  
Phenolic Components ◽  
Ethanolic Extracts ◽  
Arabis Alpina ◽  
Ethanol Extracts ◽  
Stem Leaf

AbstractIn this study, in vitro biological activities of both methanol and ethanol extracts of Arabis alpina subsp. brevifolia were investigated. Also, the phenolic components of this plant was examined in this study. The extracts were tested against the eight strains of food pathogens for their antimicrobial activities by utilizing minimum inhibitory concentration (MIC) and disc diffusion assay. The non-enzymatic antioxidant activities were determined according to scavenging of the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). The phenolic compounds were analyzed by high performance liquid chromatography (HPLC). The main component was ellagic acid for the methanol extract of stem-leaf, rutin for the ethanol extract of stem-leaf, and 2,5-dihydroxybenzoic acid for the methanol and ethanol extracts of fruit-flower. The ethanolic extracts of leaves revealed antibacterial activities against Salmonella Typhimurium (7 mm) while the ethanolic extracts of flowers demonstrated no activity against the test pathogens. The methanolic extracts of leaf-flower showed antibacterial activities against S. Typhimurium (7 mm). No activity was observed against C. albicans. The MIC value for four test bacteria was 13000 μg/mL. The ethanol extracts of A. alpina subsp. brevifolia exhibited the highest DPPH inhibition (76%). This study showed that A. alpina subsp. brevifolia possesses antioxidant and antimicrobial activities.

scholarly journals Characteristics, Phytochemical Analysis and Biological Activities of Extracts from Tunisian ChetouiOlea europaeaVariety

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Ines Khlif ◽  
Karim Jellali ◽  
Thomas Michel ◽  
Maria Halabalaki ◽  
Alexios Leandros Skaltsounis ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
High Performance ◽  
Biological Activities ◽  
Antioxidant Property ◽  
Antimicrobial Activities ◽  
Phytochemical Analysis ◽  
Polar Extracts ◽  
Layer Chromatography

This study selected 10 extracts from Tunisian chetouiO. europaeavariety for their total phenolics, flavonoids, and phytochemical analyses as well as for their antioxidant and antimicrobial activities determination. Thein vitroantioxidant property was investigated using DPPH, ferric reducing antioxidant capacity (FRAP), oxygen reducing antioxidant capacity (ORAC), andβ-carotene-linoleic acid bleaching assays while antimicrobial activity was evaluated using macrodilutions method. For all organs of chetouiO. europaeavariety, the investigated activities were found to be higher in the polar extracts (ethyl acetate, methanol, and methanol/water). These activities were correlated with the presence of phenolic compounds. Phytochemical analyses revealed that the crude extracts contain triterpenoids, quinones, and flavonoids. High performance liquid chromatography (HPLC) and high performance thin layer chromatography (HPTLC) confirmed the presence of phenolic compounds in the studied extracts.

scholarly journals Identification and Quantification of Coumarins by UHPLC-MS in Arabidopsis Thaliana Natural Populations

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1804
Author(s):  
Izabela Perkowska ◽  
Joanna Siwinska ◽  
Alexandre Olry ◽  
Jérémy Grosjean ◽  
Alain Hehn ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
High Performance ◽  
Biological Activities ◽  
Natural Populations ◽  
Biologically Active ◽  
Stress Factors ◽  
Mass Spectrometry Analysis ◽  
Engineering Research ◽  
Spectrometry Analysis ◽  
Model Plant Arabidopsis Thaliana

Coumarins are phytochemicals occurring in the plant kingdom, which biosynthesis is induced under various stress factors. They belong to the wide class of specialized metabolites well known for their beneficial properties. Due to their high and wide biological activities, coumarins are important not only for the survival of plants in changing environmental conditions, but are of great importance in the pharmaceutical industry and are an active source for drug development. The identification of coumarins from natural sources has been reported for different plant species including a model plant Arabidopsis thaliana. In our previous work, we demonstrated a presence of naturally occurring intraspecies variation in the concentrations of scopoletin and its glycoside, scopolin, the major coumarins accumulating in Arabidopsis roots. Here, we expanded this work by examining a larger group of 28 Arabidopsis natural populations (called accessions) and by extracting and analysing coumarins from two different types of tissues–roots and leaves. In the current work, by quantifying the coumarin content in plant extracts with ultra-high-performance liquid chromatography coupled with a mass spectrometry analysis (UHPLC-MS), we detected a significant natural variation in the content of simple coumarins like scopoletin, umbelliferone and esculetin together with their glycosides: scopolin, skimmin and esculin, respectively. Increasing our knowledge of coumarin accumulation in Arabidopsis natural populations, might be beneficial for the future discovery of physiological mechanisms of action of various alleles involved in their biosynthesis. A better understanding of biosynthetic pathways of biologically active compounds is the prerequisite step in undertaking a metabolic engineering research.

scholarly journals Phytochemical Characterization and Evaluation of Biological Activities of Egyptian Carob Pods (Ceratonia siliqua L.) Aqueous Extract: In Vitro Study

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2626
Author(s):  
Wael Sobhy Darwish ◽  
Abada El Sayed Khadr ◽  
Maher Abd El Naby Kamel ◽  
Mabrouk A. Abd Eldaim ◽  
Ibrahim El Tantawy El Sayed ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Biologically Active ◽  
Phytochemical Analysis ◽  
Type I ◽  
Hypotonic Solution ◽  
Ceratonia Siliqua

Ceratonia siliqua (Carob) is an evergreen Mediterranean tree, and carob pods are potentially nutritive and have medicinal value. The present study was carried out to estimate the possible biological activities of phytochemical-characterized carob pod aqueous extract (CPAE). The phytochemical contents of CPAE were determined by using colorimetric methods and HPLC. In addition, the free radical scavenging properties and anti-diabetic, anti-hemolytic, and antimicrobial activities were estimated by using standardized in vitro protocols. The phytochemical analysis revealed that CPAE was rich in polyphenols, flavonoids, and alkaloids, where it contained a significant amount of gallic acid, catechin, and protocatechuic acid. Furthermore, CPAE exhibited strong antioxidant activity where it prevented the formation of 2, 2-Diphenyl-1-picryl hydrazyl, hydroxyl, and nitric oxide free radicals. Additionally, it had a potent inhibitory effect against digestive enzymes (amylase, maltase, sucrase, and lactase). Moreover, CPAE exhibited anti-Staph aureus, anti-Escherichia coli, anti-Candida albicans, and anti-herpes simplex type I virus (HSV-I). Finally, CPAE protected the erythrocyte membrane from hypotonic solution-induced hemolysis. Altogether, CPAE could be regarded as an interesting source of biologically active antioxidant, anti-diabetic, and antimicrobial preparation for a potential application in pharmaceutical and food supplement fields.

scholarly journals Antioxidant activities and A549 lung adenocarcinomic cells against capacity from various extracts of Agrimonia pilosa Ledeb

2018 ◽  
Vol 4 (3) ◽  
pp. 114-119
Author(s):  
Quang Ung Le ◽  
◽  
Horng Liang Lay ◽  
Ming Chang Wu ◽  
◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
A549 Cells ◽  
Total Phenolic ◽  
Root Extract ◽  
Inhibitory Capacity ◽  
Ethanol Extracts

Agrimonia pilosa Ledeb (AL) has received considerable attention as a herbal medicine for its applications in ethnopharmacology with heath benefits. This study aimed to investigate antioxidant activities and A549 growth inhibitory capacity from its root extract (RE) and aerial parts extract (AE). The 50% ethanol extracts were used for the tests. The total polyphenol content and the antioxidant effects comprising ABTS+ and DPPH free radical scavenging activities were evaluated. Phenolic compounds in the extracts were isolated using high performance liquid chromatography (HPLC). Lactate dehydrogenase released in medium was also evaluated. Total phenolic and flavonoid content, and in vitro antioxidant potential of the RE were higher (p<0.01) than that of the AE. Two individual phenolic compounds consisting of 4-hydroxybenzoic acid and p-coumaric acid were firstly identified in both by HPLC. The RE exhibited higher A549 inhibitory capacity compared to the AE and activated the apoptotic proteins of bcl-2, bax, bad, caspase-3 and caspase-9 in A549. In conclusion, the AL extracts were more effective in antioxidant and A549 cells inhibitory capacity.

scholarly journals Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen

Biomolecules ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 783 ◽  
Author(s):  
Mirjana Mosić ◽  
Jelena Trifković ◽  
Irena Vovk ◽  
Uroš Gašić ◽  
Živoslav Tešić ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Ion Trap ◽  
Therapeutic Potential ◽  
Flavonoid Glycosides ◽  
Phenolic Composition ◽  
Bee Pollen ◽  
Orbitrap Mass Spectrometry ◽  
Health Promoting ◽  
First Time

Information on compositional, nutritional and functional properties of bee-pollen, as a health-promoting food, is essential for defining its quality. Concerning the nutritional importance of phenolic compounds, the aim of this study was to determine the phenolic profile and antioxidant activity of twenty-four bee-pollen samples collected from different regions of Serbia. High-performance thin-layer chromatographic (HPTLC) fingerprinting was used for profiling of bee-pollen samples according to the botanical type. HPTLC hyphenated with image analysis and a pattern recognition technique confirmed the grouping of samples caused by the specific phenolic composition of pollens of different botanical origin. Flavonoid glycosides in bee-pollen samples were identified by applying ultra-high-performance liquid chromatography (UHPLC) coupled with linear ion trap-Orbitrap mass spectrometry (LTQ Orbitrap MS). Eight out of twenty-seven flavonol glycosides were identified in bee-pollen samples for the first time. All analyzed bee-pollen samples showed a high number of phenolic compounds which may have therapeutic potential.

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