scholarly journals Distinct Transcriptional Programs Underlie Differences in Virulence of Isolates on Host Plants in a Fungal Pathogen, Colletotrichum gloeosporioides

2021 ◽  
Vol 12 ◽  
Author(s):  
Wonsu Cheon ◽  
Young Soo Kim ◽  
Kotnala Balaraju ◽  
Younmi Lee ◽  
Hyeok Tae Kwon ◽  
...  
Keyword(s):  
Fungal Pathogens ◽  
Host Plants ◽  
Colletotrichum Gloeosporioides ◽  
Isocitrate Lyase ◽  
Glyoxylate Cycle ◽  
Virulence Gene ◽  
Rna Seq ◽  
Susceptible Host ◽  
Factors Affecting ◽  
Different Types

Susceptible host plants challenged by fungal pathogens can display different types of lesions, which can be attributed to environmental factors affecting the nature of interactions between the host and pathogen. During our survey of apple anthracnose in Korea, two distinct types of disease symptoms, designated as progressive (PS) and static symptoms (SS), were recognized. PS is a typical, rapidly enlarging symptom of apple anthracnose, while SS is a small, dark speck that does not expand further until the harvesting season. Isolation and genotyping of pathogens from disease lesions suggested that all of them belong to Colletotrichum gloeosporioides, a well-known causal agent of apple anthracnose. Two types of isolates were comparable in growth on media, spore germination and appressorium formation, virulence test on fruits at various temperature conditions. Furthermore, they were analyzed at the molecular level by a phylogenetic tree, RNA-seq, and expression of virulence gene. However, the SS isolates were defective in appressorium-mediated penetration into the underlying substratum. RNA-seq analysis of PS and SS isolates showed that distinct transcriptional programs underlie the development of different types of anthracnose symptoms in host plants. One downregulated gene in SS encoded isocitrate lyase is essential for disease development via its involvement in the glyoxylate cycle. It partly explains why SS is less virulent than PS on host plants. Overall, our work challenges the traditional view on the development of different lesion types and provides valuable insights into variations that exist in the pathogen population.

scholarly journals Innate Resistance and Phosphite Treatment Affect Both the Pathogen’s and Host’s Transcriptomes in the Tanoak-Phytophthora ramorum Pathosystem

2021 ◽  
Vol 7 (3) ◽  
pp. 198
Author(s):  
Takao Kasuga ◽  
Katherine J. Hayden ◽  
Catherine A. Eyre ◽  
Peter J. P. Croucher ◽  
Shannon Schechter ◽  
...  
Keyword(s):  
Treatment Group ◽  
Mechanism Of Action ◽  
Mode Of Action ◽  
Host Plants ◽  
Phytophthora Ramorum ◽  
Sudden Oak Death ◽  
Inoculation Experiment ◽  
Rna Seq ◽  
Susceptible Host ◽  
Innate Resistance

Phosphites have been used to control Sudden Oak Death; however, their precise mode of action is not fully understood. To study the mechanism of action of phosphites, we conducted an inoculation experiment on two open-pollinated tanoak families, previously found to be partially resistant. Stems of treatment group individuals were sprayed with phosphite, and seven days later, distal leaves were inoculated with the Sudden Oak Death pathogen Phytophthora ramorum. Leaves from treated and untreated control plants were harvested before and seven days after inoculation, and transcriptomes of both host and pathogen were analyzed. We found that tanoak families differed in the presence of innate resistance (resistance displayed by untreated tanoak) and in the response to phosphite treatment. A set of expressed genes associated with innate resistance was found to overlap with an expressed gene set for phosphite-induced resistance. This observation may indicate that phosphite treatment increases the resistance of susceptible host plants. In addition, genes of the pathogen involved in detoxification were upregulated in phosphite-treated plants compared to phosphite-untreated plants. In summary, our RNA-Seq analysis supports a two-fold mode of action of phosphites, including a direct toxic effect on P. ramorum and an indirect enhancement of resistance in the tanoak host.

scholarly journals Regulation of Oxalate Metabolism in Spinach Revealed by RNA-Seq-Based Transcriptomic Analysis

2021 ◽  
Vol 22 (10) ◽  
pp. 5294
Author(s):  
Vijay Joshi ◽  
Arianne Penalosa ◽  
Madhumita Joshi ◽  
Sierra Rodriguez
Keyword(s):  
Spinacia Oleracea ◽  
Isocitrate Lyase ◽  
Expression Profiles ◽  
Glyoxylate Cycle ◽  
Leafy Vegetables ◽  
Rna Seq ◽  
Physiological Processes ◽  
Spinacia Oleracea L ◽  
Root Tissues ◽  
Oxalate Metabolism

Although spinach (Spinacia oleracea L.) is considered to be one of the most nutrient-rich leafy vegetables, it is also a potent accumulator of anti-nutritional oxalate. Reducing oxalate content would increase the nutritional value of spinach by enhancing the dietary bioavailability of calcium and other minerals. This study aimed to investigate the proposed hypothesis that a complex network of genes associated with intrinsic metabolic and physiological processes regulates oxalate homeostasis in spinach. Transcriptomic (RNA-Seq) analysis of the leaf and root tissues of two spinach genotypes with contrasting oxalate phenotypes was performed under normal physiological conditions. A total of 2308 leaf- and 1686 root-specific differentially expressed genes (DEGs) were identified in the high-oxalate spinach genotype. Gene Ontology (GO) analysis of DEGs identified molecular functions associated with various enzymatic activities, while KEGG pathway analysis revealed enrichment of the metabolic and secondary metabolite pathways. The expression profiles of genes associated with distinct physiological processes suggested that the glyoxylate cycle, ascorbate degradation, and photorespiratory pathway may collectively regulate oxalate in spinach. The data support the idea that isocitrate lyase (ICL), ascorbate catabolism-related genes, and acyl-activating enzyme 3 (AAE3) all play roles in oxalate homeostasis in spinach. The findings from this study provide the foundation for novel insights into oxalate metabolism in spinach.

Control of Winged Waterprimrose (Jussiaea decurrens) and Northern Jointvetch (Aeschynomene virginica) with Fungal Pathogens

Weed Science ◽  
1979 ◽  
Vol 27 (5) ◽  
pp. 497-501 ◽  
Author(s):  
C. D. Boyette ◽  
G. E. Templeton ◽  
R. J. Smith
Keyword(s):  
Oryza Sativa ◽  
Glycine Max ◽  
Fungal Pathogens ◽  
Liquid Culture ◽  
Field Experiments ◽  
Colletotrichum Gloeosporioides ◽  
Pathogenic Fungus ◽  
Field Tests ◽  
Wide Range ◽  
Growing Region

An indigenous, host-specific, pathogenic fungus that parasitizes winged waterprimrose [Jussiaea decurrens(Walt.) DC.] is endemic in the rice growing region of Arkansas. The fungus was isolated and identified asColletotrichum gloeosporioides(Penz.) Sacc. f.sp. jussiaeae(CGJ). It is highly specific for parasitism of winged waterprimrose and not parasitic on creeping waterprimrose (J. repensL. var.glabrescensKtze.), rice (Oryza sativaL.), soybeans [Glycine max(L.) Merr.], cotton (Gossypium hirsutumL.), or 4 other crops and 13 other weeds. The fungus was physiologically distinct from C.gloeosporioides(Penz.) Sacc. f. sp.aeschynomene(CGA), an endemic anthracnose pathogen of northern jointvetch[Aeschynomene virginica(L.) B.S.P.], as indicated by cross inoculations of both weeds. Culture in the laboratory and inoculation of winged waterprimrose in greenhouse, growth chamber and field experiments indicated that the pathogen was stable, specific, and virulent in a wide range of environments. The pathogen yielded large quantities of spores in liquid culture. It is suitable for control of winged waterprimrose. Winged waterprimrose and northern jointvetch were controlled in greenhouse and field tests by application of spore mixtures of CGJ and CGA at concentrations of 1 to 2 million spores/ml of each fungus in 94 L/ha of water; the fungi did not damage rice or nontarget crops.

scholarly journals Transcriptional and morphological profiling of parvalbumin interneuron subpopulations in the mouse hippocampus

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Lin Que ◽  
David Lukacsovich ◽  
Wenshu Luo ◽  
Csaba Földy
Keyword(s):  
Large Scale ◽  
Cell Types ◽  
Rna Seq ◽  
Neuronal Identity ◽  
Parvalbumin Interneurons ◽  
Different Types ◽  
Parvalbumin Interneuron ◽  
Cam Profile ◽  
Developmental Domains

AbstractThe diversity reflected by >100 different neural cell types fundamentally contributes to brain function and a central idea is that neuronal identity can be inferred from genetic information. Recent large-scale transcriptomic assays seem to confirm this hypothesis, but a lack of morphological information has limited the identification of several known cell types. In this study, we used single-cell RNA-seq in morphologically identified parvalbumin interneurons (PV-INs), and studied their transcriptomic states in the morphological, physiological, and developmental domains. Overall, we find high transcriptomic similarity among PV-INs, with few genes showing divergent expression between morphologically different types. Furthermore, PV-INs show a uniform synaptic cell adhesion molecule (CAM) profile, suggesting that CAM expression in mature PV cells does not reflect wiring specificity after development. Together, our results suggest that while PV-INs differ in anatomy and in vivo activity, their continuous transcriptomic and homogenous biophysical landscapes are not predictive of these distinct identities.

scholarly journals Factors affecting the amount of infection obtained by aphis transmission of the virus Hy. III

1936 ◽  
Vol 226 (540) ◽  
pp. 457-489 ◽  
Keyword(s):  
Plant Virus ◽  
Host Plants ◽  
Suitable Host ◽  
Factors Affecting

Much of the information which can be obtained about a plant virus agent is ultimately derived from the quantity as well as the type of the infections resulting from inoculations to suitable host plants. The number ofinfections obtained does not depend solely on the nature of the particular virus concerned. It is dependent on other variable factors, such as the efficiency of the means of infection introducing the virus, the susceptibility of the plants receiving it, and the concentration of the virus in the source from which it was obtained. In this paper a'n attempt has been made to estimate the effect of some of these variables on infection by insects.

Co-ordinate regulation of genes involved in storage lipid mobilization in Arabidopsis thaliana

2001 ◽  
Vol 29 (2) ◽  
pp. 283-286 ◽  
Author(s):  
E. L. Rylott ◽  
M. A. Hooks ◽  
I. A. Graham
Keyword(s):  
Arabidopsis Thaliana ◽  
Enzyme Activities ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Isocitrate Lyase ◽  
Glyoxylate Cycle ◽  
Molecular Genetic ◽  
Regulation Of Gene Expression ◽  
Growth Period ◽  
Malate Synthase ◽  
The Glyoxylate Cycle

Molecular genetic approaches in the model plant Arabidopsis thaliana (ColO) are shedding new light on the role and control of the pathways associated with the mobilization of lipid reserves during oilseed germination and post-germinative growth. Numerous independent studies have reported on the expression of individual genes encoding enzymes from the three major pathways: β-oxidation, the glyoxylate cycle and gluconeogenesis. However, a single comprehensive study of representative genes and enzymes from the different pathways in a single plant species has not been done. Here we present results from Arabidopsis that demonstrate the co-ordinate regulation of gene expression and enzyme activities for the acyl-CoA oxidase- and 3-ketoacyl-CoA thiolasemediated steps of β-oxidation, the isocitrate lyase and malate synthase steps of the glyoxylate cycle and the phosphoenolpyruvate carboxykinase step of gluconeogenesis. The mRNA abundance and enzyme activities increase to a peak at stage 2, 48 h after the onset of seed germination, and decline thereafter either to undetectable levels (for malate synthase and isocitrate lyase) or low basal levels (for the genes of β-oxidation and gluconeogenesis). The co-ordinate induction of all these genes at the onset of germination raises the possibility that a global regulatory mechanism operates to induce the expression of genes associated with the mobilization of storage reserves during the heterotrophic growth period.

Isocitrate lyase fromAspergillus nidulans: crystallization and X-ray analysis of a glyoxylate cycle enzyme

1997 ◽  
Vol 53 (4) ◽  
pp. 488-490 ◽  
Author(s):  
S. J. Langridge ◽  
P. J. Baker ◽  
J. R. De Lucas ◽  
S. E. Sedelnikova ◽  
G. Turner ◽  
...  
Keyword(s):  
Isocitrate Lyase ◽  
Glyoxylate Cycle ◽  
X Ray ◽  
Cycle Enzyme

Molecular analysis of the Penicillium marneffei glyceraldehyde-3-phosphate dehydrogenase-encoding gene (gpdA) and differential expression of gpdA and the isocitrate lyase-encoding gene (acuD) upon internalization by murine macrophages

2008 ◽  
Vol 57 (11) ◽  
pp. 1322-1328 ◽  
Author(s):  
Sophit Thirach ◽  
Chester R. Cooper ◽  
Nongnuch Vanittanakom
Keyword(s):  
Differential Expression ◽  
Isocitrate Lyase ◽  
Glyoxylate Cycle ◽  
Evolutionary Relationship ◽  
Amino Acid Sequences ◽  
Penicillium Marneffei ◽  
Macrophage Infection ◽  
Dimorphic Fungus ◽  
Isolation And Characterization ◽  
Encoding Gene

Penicillium marneffei is an intracellular dimorphic fungus that can cause a fatal disseminated disease in human immunodeficiency virus-infected patients. The factors that affect the pathogenicity of this fungus remain unclear. Here, we report the isolation and characterization of the gpdA cDNA and genomic clones encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in P. marneffei. Phylogenetic analysis of GAPDH amino acid sequences demonstrated the evolutionary relationship of P. marneffei to other fungi, including the intracellular pathogen Ajellomyces capsulatus. To assess the central importance of phagocytic cells in defence against P. marneffei infection, we used Northern blotting to investigate the response of the isocitrate lyase-encoding gene (acuD) and gpdA to nutrient deprivation inside macrophages. The results revealed that after macrophage internalization, the gene involved in the glyoxylate cycle, acuD, showed higher expression levels as early as 2 h from the start of co-incubation, and the differential expression could be observed again at 8 h after infection. In contrast, the expression of gpdA was downregulated in the yeast phase, as well as during macrophage infection after 2, 4 and 8 h of infection. The induction of P. marneffei acuD was shown to be coordinated with the downregulation of the glycolytic gpdA gene, implying that the cytoplasmic environment of macrophages is deficient in glucose and the glyoxylate pathway could be used by this pathogen to allow subsistence on two-carbon compounds within the host cell following its intracellular persistence.

scholarly journals Whole-Genome Transcriptional Profiling of Bradyrhizobium japonicum during Chemoautotrophic Growth

2008 ◽  
Vol 190 (20) ◽  
pp. 6697-6705 ◽  
Author(s):  
William L. Franck ◽  
Woo-Suk Chang ◽  
Jing Qiu ◽  
Masayuki Sugawara ◽  
Michael J. Sadowsky ◽  
...  
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Carbon Fixation ◽  
Cultured Cells ◽  
Isocitrate Lyase ◽  
Nitrogenase Activity ◽  
Glyoxylate Cycle ◽  
Transcriptional Profiling ◽  
Pentose Phosphate ◽  
Hydrogen Gas ◽  
Essential Components

ABSTRACT Bradyrhizobium japonicum is a facultative chemoautotroph capable of utilizing hydrogen gas as an electron donor in a respiratory chain terminated by oxygen to provide energy for cellular processes and carbon dioxide assimilation via a reductive pentose phosphate pathway. A transcriptomic analysis of B. japonicum cultured chemoautotrophically identified 1,485 transcripts, representing 17.5% of the genome, as differentially expressed when compared to heterotrophic cultures. Genetic determinants required for hydrogen utilization and carbon fixation, including the uptake hydrogenase system and components of the Calvin-Benson-Bassham cycle, were strongly induced in chemoautotrophically cultured cells. A putative isocitrate lyase (aceA; blr2455) was among the most strongly upregulated genes, suggesting a role for the glyoxylate cycle during chemoautotrophic growth. Addition of arabinose to chemoautotrophic cultures of B. japonicum did not significantly alter transcript profiles. Furthermore, a subset of nitrogen fixation genes was moderately induced during chemoautotrophic growth. In order to specifically address the role of isocitrate lyase and nitrogenase in chemoautotrophic growth, we cultured aceA, nifD, and nifH mutants under chemoautotrophic conditions. Growth of each mutant was similar to that of the wild type, indicating that the glyoxylate bypass and nitrogenase activity are not essential components of chemoautotrophy in B. japonicum.

Deceleration of sliding motorcycles (mainly scooters) in accident reconstructions

2017 ◽  
Vol 232 (7) ◽  
pp. 866-876 ◽  
Author(s):  
Carlo Cialdai ◽  
Dario Vangi ◽  
Antonio Virga
Keyword(s):  
Urban Areas ◽  
Test Session ◽  
Road Accidents ◽  
Factors Affecting ◽  
Different Types ◽  
Energy Dissipated ◽  
Comparison Of The Results ◽  
Two Wheeler ◽  
The Impact ◽  
Good Agreement

This paper presents an analysis of the situation in which a two-wheeler (i.e. a motorcycle, where the term motorcycles includes scooters) falls over to the side and then successively slides; this typically occurs in road accidents involving this type of vehicle. Knowing the deceleration rate of the sliding phase allows the kinetic energy dissipated and the speed of the motorcycle just before the fall to the ground to be calculated. These parameters are very important in the analysis and reconstruction of accidents. The work presented in this paper was developed in two experimental test sessions on fully faired motorcycles which are mainly of the scooter type and widely used in urban areas. In the first session, sliding tests were carried out, with the speed in the range 10–50 km/h, on three different types of road surface. Analysis of the evidence allowed the dissipative main phases of motion of the motorcycle (the impact with the ground, the rebounds and the stabilized swiping) to be identified and some factors affecting the phenomenon to be studied. The coefficient of average deceleration was calculated using two typical equations. The second test session consisted of drag tests. In these tests, the motorcycle, which had previously laid on its side, was dragged for a few metres at a constant speed of about 20 km/h, while the drag force was measured. A comparison of the results obtained in these tests with those obtained in the sliding tests yielded very good agreement in the coefficients of deceleration.

Sign in / Sign up

Export Citation Format

Share Document