scholarly journals Drought Stress Triggers Shifts in the Root Microbial Community and Alters Functional Categories in the Microbial Gene Pool

2021 ◽  
Vol 12 ◽  
Author(s):  
Jianbo Xie ◽  
Ghada E. Dawwam ◽  
Amira E. Sehim ◽  
Xian Li ◽  
Jiadong Wu ◽  
...  
Keyword(s):  
Microbial Community ◽  
Drought Stress ◽  
Plant Growth ◽  
Crop Productivity ◽  
Plant Tolerance ◽  
Functional Categories ◽  
Crop Failure ◽  
Drought Treatment ◽  
The Impact ◽  
Root Microbiome

Drought is a major threat to crop productivity and causes decreased plant growth, poor yields, and crop failure. Nevertheless, the frequency of droughts is expected to increase in the coming decades. The microbial communities associated with crop plants can influence how plants respond to various stresses; hence, microbiome manipulation is fast becoming an effective strategy for improving the stress tolerance of plants. The effect of drought stress on the root microbiome of perennial woody plants is currently poorly understood. Using Populus trees as a model ecosystem, we found that the diversity of the root microbial community decreased during drought treatment and that compositional shifts in microbes during drought stress were driven by the relative abundances of a large number of dominant phyla, including Actinobacteria, Firmicutes, and Proteobacteria. A subset of microbes, including Streptomyces rochei, Bacillus arbutinivorans, B. endophyticus, B. megaterium, Aspergillus terreus, Penicillium raperi, Trichoderma ghanense, Gongronella butleri, and Rhizopus stolonifer, was isolated from the drought-treated poplar rhizosphere soils, which have potentially beneficial to plant fitness. Further controlled inoculation experiments showed that the isolated bacterial and fungal isolates positively impacted plant growth and drought tolerance. Collectively, our results demonstrate the impact of drought on root microbiome structure and provide a novel example of manipulating root microbiomes to improve plant tolerance.

scholarly journals Physiological and proteomic responses to drought stress in leaves of Amygdalus Mira (Koehne) Yü et Lu

2019 ◽  
Author(s):  
Liping Xu ◽  
Guangze Jin ◽  
Fachun Guan ◽  
Qiuxiang Luo ◽  
Fan Juan Meng
Keyword(s):  
Drought Stress ◽  
Plant Growth ◽  
The Tibetan Plateau ◽  
Functional Categories ◽  
Drought Treatment ◽  
Gas Exchanges ◽  
Transport Stress ◽  
Proteomic Responses ◽  
Cytoskeleton Dynamics ◽  
Related Proteins

Abstract Background:Plant development is strongly influenced by various stresses, such as drought and salinity. Drought is a serious threat which can reduce agricultural productivity and obstruct plant growth. Although the mechanism of plants adapted to drought stress has been studied extensively, the adaptive strategies of Amygdalus Mira (Koehne) Yü et Lu grown in drought and re-watered habitats remain undefined. In this paper, A. Mira from the Tibetan Plateau have outstanding environmental, economic, nutritional and medicinal values, and can thrive in extreme drought. Results:This paper investigated physiological and proteomic responses in leaves of A. Mira during the period of drought stress and recovery, to understand their strategies mechanism. The changes of plant growth, photosynthesis, enzymes and non-enzymatic antioxidant during drought and re-watering were analyzed in leaves. Compared with controls, A. Mira showed stronger adaptive and resistant characteristics to drought stress. Proteomic technique was also be used to study mechanisms of drought tolerance in A. Mira leaves. Differentially expressed proteins were identified using mass spectrometry. Accordingly, 103 proteins involved in 10 functional categories: Cytoskeleton dynamics, Energy metabolism, Carbohydrate metabolism, Photosynthesis, Transcription and translation, Transport, Stress and defense, Molecular chaperones, Other materials metabolism, and Unknown function were identified. These results showed that increase of stress-defense-related proteins in leaves after drought treatment were contributed to cope with drought stress. Importantly, A. Mira developed adaptive mechanism to scavenge reactive oxygen species (ROS), including enhancement of antioxidant enzymes activities and non-enzymatic low molecular, reduction of energy, and efficiency of adjusting gas exchanges. Conclusions:These results may help improve understanding concerning the adaptation of A. Mira to drought.

scholarly journals Drought delays development of the sorghum root microbiome and enriches for monoderm bacteria

2018 ◽  
Vol 115 (18) ◽  
pp. E4284-E4293 ◽  
Author(s):  
Ling Xu ◽  
Dan Naylor ◽  
Zhaobin Dong ◽  
Tuesday Simmons ◽  
Grady Pierroz ◽  
...  
Keyword(s):  
Drought Stress ◽  
Plant Growth ◽  
Crop Productivity ◽  
Outer Cell ◽  
Negative Effects ◽  
Temporal Sampling ◽  
Effects Of Drought ◽  
Abc Transporter Genes ◽  
Increased Activity ◽  
Root Microbiome

Drought stress is a major obstacle to crop productivity, and the severity and frequency of drought are expected to increase in the coming century. Certain root-associated bacteria have been shown to mitigate the negative effects of drought stress on plant growth, and manipulation of the crop microbiome is an emerging strategy for overcoming drought stress in agricultural systems, yet the effect of drought on the development of the root microbiome is poorly understood. Through 16S rRNA amplicon and metatranscriptome sequencing, as well as root metabolomics, we demonstrate that drought delays the development of the early sorghum root microbiome and causes increased abundance and activity of monoderm bacteria, which lack an outer cell membrane and contain thick cell walls. Our data suggest that altered plant metabolism and increased activity of bacterial ATP-binding cassette (ABC) transporter genes are correlated with these shifts in community composition. Finally, inoculation experiments with monoderm isolates indicate that increased colonization of the root during drought can positively impact plant growth. Collectively, these results demonstrate the role that drought plays in restructuring the root microbiome and highlight the importance of temporal sampling when studying plant-associated microbiomes.

scholarly journals Ameliorative effect of melatonin improves drought tolerance by regulating growth, photosynthetic traits and leaf ultrastructure of maize seedlings

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Shakeel Ahmad ◽  
Ihsan Muhammad ◽  
Guo Yun Wang ◽  
Muhammad Zeeshan ◽  
Li Yang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Plant Growth ◽  
Photosynthetic Pigments ◽  
Field Capacity ◽  
Maize Seedling ◽  
Stress Conditions ◽  
Antioxidant Enzyme Activities ◽  
Plant Tolerance ◽  
Maize Seedlings ◽  
The Impact

Abstract Background Melatonin is considered a potential plant growth regulator to enhance the growth of plants and increase tolerance to various abiotic stresses. Nevertheless, melatonin’s role in mediating stress response in different plant species and growth cycles still needs to be explored. This study was conducted to understand the impact of different melatonin concentrations (0, 50, 100, and 150 μM) applied as a soil drench to maize seedling under drought stress conditions. A decreased irrigation approach based on watering was exposed to maize seedling after drought stress was applied at 40–45% of field capacity. Results The results showed that drought stress negatively affected the growth behavior of maize seedlings, such as reduced biomass accumulation, decreased photosynthetic pigments, and enhanced the malondialdehyde and reactive oxygen species (ROS). However, melatonin application enhanced plant growth; alleviated ROS-induced oxidative damages by increasing the photosynthetic pigments, antioxidant enzyme activities, relative water content, and osmo-protectants of maize seedlings. Conclusions Melatonin treatment also enhanced the stomatal traits, such as stomatal length, width, area, and the number of pores under drought stress conditions. Our data suggested that 100 μM melatonin application as soil drenching could provide a valuable foundation for improving plant tolerance to drought stress conditions.

scholarly journals Comparing transcriptional responses to Fusarium crown rot in wheat and barley identified an important relationship between disease resistance and drought tolerance

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Z. Y. Su ◽  
J. J. Powell ◽  
S. Gao ◽  
M. Zhou ◽  
C. Liu
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Crown Rot ◽  
Differentially Expressed ◽  
Transcriptional Responses ◽  
Drought Treatment ◽  
Crop Species ◽  
Fusarium Crown Rot ◽  
Important Relationship ◽  
The Impact

Abstract Background Fusarium crown rot (FCR) is a chronic disease in cereal production worldwide. The impact of this disease is highly environmentally dependant and significant yield losses occur mainly in drought-affected crops. Results In the study reported here, we evaluated possible relationships between genes conferring FCR resistance and drought tolerance using two approaches. The first approach studied FCR induced differentially expressed genes (DEGs) targeting two barley and one wheat loci against a panel of genes curated from the literature based on known functions in drought tolerance. Of the 149 curated genes, 61.0% were responsive to FCR infection across the three loci. The second approach was a comparison of the global DEGs induced by FCR infection with the global transcriptomic responses under drought in wheat. This analysis found that approximately 48.0% of the DEGs detected one week following drought treatment and 74.4% of the DEGs detected three weeks following drought treatment were also differentially expressed between the susceptible and resistant isolines under FCR infection at one or more timepoints. As for the results from the first approach, the vast majority of common DEGs were downregulated under drought and expressed more highly in the resistant isoline than the sensitive isoline under FCR infection. Conclusions Results from this study suggest that the resistant isoline in wheat was experiencing less drought stress, which could contribute to the stronger defence response than the sensitive isoline. However, most of the genes induced by drought stress in barley were more highly expressed in the susceptible isolines than the resistant isolines under infection, indicating that genes conferring drought tolerance and FCR resistance may interact differently between these two crop species. Nevertheless, the strong relationship between FCR resistance and drought responsiveness provides further evidence indicating the possibility to enhance FCR resistance by manipulating genes conferring drought tolerance.

scholarly journals The Arabidopsis Cys2/His2 zinc finger transcription factor ZAT18 is a positive regulator of plant tolerance to drought stress

2017 ◽  
Vol 68 (11) ◽  
pp. 2991-3005 ◽  
Author(s):  
Mingzhu Yin ◽  
Yanping Wang ◽  
Lihua Zhang ◽  
Jinzhu Li ◽  
Wenli Quan ◽  
...  
Keyword(s):  
Drought Stress ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Leaf Water ◽  
Pathway Enrichment Analysis ◽  
Antioxidant Enzyme Activities ◽  
Sequencing Analysis ◽  
Plant Tolerance ◽  
Drought Treatment ◽  
Positive Regulator

Abstract Environmental stress poses a global threat to plant growth and reproduction, especially drought stress. Zinc finger proteins comprise a family of transcription factors that play essential roles in response to various abiotic stresses. Here, we found that ZAT18 (At3g53600), a nuclear C2H2 zinc finger protein, was transcriptionally induced by dehydration stress. Overexpression (OE) of ZAT18 in Arabidopsis improved drought tolerance while mutation of ZAT18 resulted in decreased plant tolerance to drought stress. ZAT18 was preferentially expressed in stems, siliques, and vegetative rosette leaves. Subcellular location results revealed that ZAT18 protein was predominantly localized in the nucleus. ZAT18 OE plants exhibited less leaf water loss, lower content of reactive oxygen species (ROS), higher leaf water content, and higher antioxidant enzyme activities after drought treatment when compared with the wild type (WT). RNA sequencing analysis showed that 423 and 561 genes were transcriptionally modulated by the ZAT18 transgene before and after drought treatment, respectively. Pathway enrichment analysis indicated that hormone metabolism, stress, and signaling were over-represented in ZAT18 OE lines. Several stress-responsive genes including COR47, ERD7, LEA6, and RAS1, and hormone signaling transduction-related genes including JAZ7 and PYL5 were identified as putative target genes of ZAT18. Taken together, ZAT18 functions as a positive regulator and plays a crucial role in the plant response to drought stress.

scholarly journals What is the Difference between the Response of Grass Pea (Lathyrus sativus L.) to Salinity and Drought Stress?—A Physiological Study

Agronomy ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 833 ◽  
Author(s):  
Barbara Tokarz ◽  
Tomasz Wójtowicz ◽  
Wojciech Makowski ◽  
Roman J. Jędrzejczyk ◽  
Krzysztof M. Tokarz
Keyword(s):  
Drought Stress ◽  
Crop Productivity ◽  
Lathyrus Sativus ◽  
Soil Drought ◽  
Severe Drought ◽  
Chemical Stress ◽  
Plant Tolerance ◽  
Grass Pea ◽  
Physiological Level ◽  
Lathyrus Sativus L

Understanding the mechanisms of plant tolerance to osmotic and chemical stress is fundamental to maintaining high crop productivity. Soil drought often occurs in combination with physiological drought, which causes chemical stress due to high concentrations of ions. Hence, it is often assumed that the acclimatization of plants to salinity and drought follows the same mechanisms. Grass pea (Lathyrus sativus L.) is a legume plant with extraordinary tolerance to severe drought and moderate salinity. The aim of the presented study was to compare acclimatization strategies of grass pea seedlings to osmotic (PEG) and chemical (NaCl) stress on a physiological level. Concentrations of NaCl and PEG were adjusted to create an osmotic potential of a medium at the level of 0.0, −0.45 and −0.65 MPa. The seedlings on the media with PEG were much smaller than those growing in the presence of NaCl, but had a significantly higher content percentage of dry weight. Moreover, the stressors triggered different accumulation patterns of phenolic compounds, soluble and insoluble sugars, proline and β-N-oxalyl-L-α,β-diamino propionic acid, as well as peroxidase and catalase activity. Our results showed that drought stress induced a resistance mechanism consisting of growth rate limitation in favor of osmotic adjustment, while salinity stress induced primarily the mechanisms of efficient compartmentation of harmful ions in the roots and shoots. Furthermore, our results indicated that grass pea plants differed in their response to drought and salinity from the very beginning of stress occurrence.

scholarly journals Green Synthesized Metal Oxide Nanoparticles Mediate Growth Regulation and Physiology of Crop Plants under Drought Stress

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1730
Author(s):  
Nadiyah M. Alabdallah ◽  
Md. Mahadi Hasan ◽  
Inès Hammami ◽  
Azzah Ibrahim Alghamdi ◽  
Dikhnah Alshehri ◽  
...  
Keyword(s):  
Drought Stress ◽  
Plant Growth ◽  
Metal Oxide ◽  
Crop Production ◽  
Growth Regulation ◽  
Foliar Application ◽  
Metal Oxide Nanoparticles ◽  
Crop Productivity ◽  
Crop Plants ◽  
Oxide Nanoparticles

Metal oxide nanoparticles (MONPs) are regarded as critical tools for overcoming ongoing and prospective crop productivity challenges. MONPs with distinct physiochemical characteristics boost crop production and resistance to abiotic stresses such as drought. They have recently been used to improve plant growth, physiology, and yield of a variety of crops grown in drought-stressed settings. Additionally, they mitigate drought-induced reactive oxygen species (ROS) through the aggregation of osmolytes, which results in enhanced osmotic adaptation and crop water balance. These roles of MONPs are based on their physicochemical and biological features, foliar application method, and the applied MONPs concentrations. In this review, we focused on three important metal oxide nanoparticles that are widely used in agriculture: titanium dioxide (TiO2), zinc oxide (ZnO), and iron oxide (Fe3O4). The impacts of various MONPs forms, features, and dosages on plant growth and development under drought stress are summarized and discussed. Overall, this review will contribute to our present understanding of MONPs’ effects on plants in alleviating drought stress in crop plants.

scholarly journals Changes in the Microbial Community in Soybean Plots Treated with Biochar and Poultry Litter

Agronomy ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1428
Author(s):  
Rosalie B. Calderon ◽  
Chang Yoon Jeong ◽  
Hyun-Hwoi Ku ◽  
Lyndon M. Coghill ◽  
Young Jeong Ju ◽  
...  
Keyword(s):  
Microbial Community ◽  
Microbial Community Composition ◽  
Poultry Litter ◽  
Crop Productivity ◽  
Microbial Interactions ◽  
Soil Microbiome ◽  
Research Station ◽  
Sequencing Data ◽  
Total N ◽  
The Impact

The application of organic materials that promote beneficial microbial activity is vital to maintaining soil health and crop productivity. We investigated the effect on the soil microbiome of applying biochar (BC), poultry litter (PL), and a combination of biochar and poultry litter (BC/PL) in soybean cultivation at the Red River Research Station (Bossier City, Louisiana, USA). We characterized the microbial profiles, community structure, and co-occurrence network from sequencing data to infer microbial interactions in the soil samples collected in the first and second years of each soil treatment (2016 and 2017, respectively). Our results showed that soil treatments with BC, PL, and a combination of both moderately changed the microbial community composition and structure. In particular, genera significantly affected by the different soil treatments were identified via differential abundance analysis. In addition, canonical correspondence analysis revealed that soil chemical properties, total N in the first year, and total C and pH in the second year influenced the community variability. The differentially enriched bacterial ASVs and co-occurring taxa were linked to nutrient cycling. This study provides insights into the impact of soil carbon amendment on the soil microbiome, a process which favors beneficial bacteria and promotes soybean growth.

scholarly journals Microbial Response to Phytostabilization in Mining Impacted Soils Using Maize in Conjunction with Biochar and Compost

2021 ◽  
Vol 9 (12) ◽  
pp. 2545
Author(s):  
Thomas F. Ducey ◽  
Gilbert C. Sigua ◽  
Jeffrey M. Novak ◽  
James A. Ippolito ◽  
Kurt A. Spokas ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Biomass ◽  
Plant Growth ◽  
Microbial Community Structure ◽  
Poultry Litter ◽  
Management Tool ◽  
Soil Microbial Community Structure ◽  
Poultry Litter Biochar ◽  
The Impact

Even after remediation, mining impacted soils can leave behind a landscape inhospitable to plant growth and containing residual heavy metals. While phytostabilization can be used to restore such sites by limiting heavy metal spread, it is reliant on soil capable of supporting plant growth. Manure-based biochars, coupled with compost, have demonstrated the ability to improve soil growth conditions in mine impacted soils, however there is a paucity of information regarding their influence on resident microbial populations. The objective of this study was to elucidate the impact of these soil amendments on microbial community structure and function in mine impacted soils placed under phytostabilization management with maize. To this aim, a combination of phospholipid fatty acid (PLFA) and enzymatic analyses were performed. Results indicate that microbial biomass is significantly increased upon addition of biochar and compost, with maximal microbial biomass achieved with 5% poultry litter biochar and compost (62.82 nmol g−1 dry soil). Microbial community structure was impacted by biochar type, rate of application, and compost addition, and influenced by pH (r2 = 0.778), EC (r2 = 0.467), and Mg soil concentrations (r2 = 0.453). In three of the four enzymes analyzed, poultry litter biochar treatments were observed with increased activity rates that were often significantly greater than the unamended control. Overall, enzyme activities rates were influenced by biochar type and rate, and addition of compost. These results suggest that using a combination of biochar and compost can be utilized as a management tool to support phytostabilization strategies in mining impacted soils.

scholarly journals Risk of crop failure due to compound dry and hot extremes estimated with nested copulas

2020 ◽  
Author(s):  
Andreia Filipa Silva Ribeiro ◽  
Ana Russo ◽  
Célia Marina Gouveia ◽  
Patrícia Páscoa ◽  
Jakob Zscheischler
Keyword(s):  
Heat Stress ◽  
Drought Stress ◽  
Dominant Role ◽  
Maximum Temperature ◽  
Crop Loss ◽  
Crop Failure ◽  
Spring Precipitation ◽  
Management Options ◽  
Extreme Stress ◽  
The Impact

Abstract. Drought and heat events stress agricultural systems and may threaten food security. The interaction between co-occurring drought and hot conditions is often particularly damaging to crop's health and may cause crop failure. In this context, traditional univariate analyses may not be adequate for reliable risk assessment of crop failure associated with compound hazards. Climate change exacerbates such risks due to an increase in the intensity and frequency of dry and hot events in many land regions. Here we model the trivariate dependence between spring maximum temperature, spring precipitation and wheat and barley yields, respectively, over two province clusters in Spain with nested copulas. Based on the full trivariate joint distribution, we (i) estimate the impact of compound hot and dry conditions on wheat and barley loss and (ii) estimate the additional impact due to compound hazards compared to individual hazards. We find that crop loss increases when drought- or heat-stress aggravates to compound dry and hot conditions and that an increase in the severity of compound conditions leads to larger damages. For instance, compared to moderate drought only, compound dry and hot conditions increase the likelihood of crop loss by 8 to 11 %, while when starting with moderate heat, the increase is between 19 to 29 % (depending on the cereal and region). This means that the likelihood of crop loss is driven primarily by drought stress than by heat stress, suggesting that drought plays the dominant role in the compound event, that is, drought stress does not require to be so extreme as heat stress to cause a similar damage. Furthermore, when compound dry and hot conditions aggravate from moderate to severe or extreme stress, crop loss probabilities increase 5 to 6 % and 6 to 8 %, respectively (depending on the cereal and region). Our results highlight the additional value of a trivariate approach for the estimating the compounding effects of dry and hot extremes on of crop failure risk. Therefore, this approach can effectively contribute to design management options and guide the decision-making process in agricultural practices.

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