The Arabidopsis Cys2/His2 zinc finger transcription factor ZAT18 is a positive regulator of plant tolerance to drought stress

Abstract Environmental stress poses a global threat to plant growth and reproduction, especially drought stress. Zinc finger proteins comprise a family of transcription factors that play essential roles in response to various abiotic stresses. Here, we found that ZAT18 (At3g53600), a nuclear C2H2 zinc finger protein, was transcriptionally induced by dehydration stress. Overexpression (OE) of ZAT18 in Arabidopsis improved drought tolerance while mutation of ZAT18 resulted in decreased plant tolerance to drought stress. ZAT18 was preferentially expressed in stems, siliques, and vegetative rosette leaves. Subcellular location results revealed that ZAT18 protein was predominantly localized in the nucleus. ZAT18 OE plants exhibited less leaf water loss, lower content of reactive oxygen species (ROS), higher leaf water content, and higher antioxidant enzyme activities after drought treatment when compared with the wild type (WT). RNA sequencing analysis showed that 423 and 561 genes were transcriptionally modulated by the ZAT18 transgene before and after drought treatment, respectively. Pathway enrichment analysis indicated that hormone metabolism, stress, and signaling were over-represented in ZAT18 OE lines. Several stress-responsive genes including COR47, ERD7, LEA6, and RAS1, and hormone signaling transduction-related genes including JAZ7 and PYL5 were identified as putative target genes of ZAT18. Taken together, ZAT18 functions as a positive regulator and plays a crucial role in the plant response to drought stress.

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GsZFP1, a new Cys2/His2-type zinc-finger protein, is a positive regulator of plant tolerance to cold and drought stress

Planta ◽

10.1007/s00425-011-1563-0 ◽

2011 ◽

Vol 235 (6) ◽

pp. 1141-1155 ◽

Cited By ~ 62

Author(s):

Xiao Luo ◽

Xi Bai ◽

Dan Zhu ◽

Yong Li ◽

Wei Ji ◽

...

Keyword(s):

Drought Stress ◽

Zinc Finger ◽

Zinc Finger Protein ◽

Plant Tolerance ◽

Positive Regulator ◽

Finger Protein

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Zinc-finger protein MdBBX7/MdCOL9, a target of MdMIEL1 E3 ligase, confers drought tolerance in apple

Plant Physiology ◽

10.1093/plphys/kiab420 ◽

2021 ◽

Author(s):

Pengxiang Chen ◽

Fang Zhi ◽

Xuewei Li ◽

Wenyun Shen ◽

Mingjia Yan ◽

...

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Zinc Finger ◽

Zinc Finger Protein ◽

E3 Ligase ◽

26S Proteasome ◽

Negative Regulator ◽

Binding Motif ◽

Ethylene Response Factor ◽

Finger Protein

Abstract Water deficit is one of the main challenges for apple (Malus × domestica) growth and productivity. Breeding drought-tolerant cultivars depends on a thorough understanding of the drought responses of apple trees. Here, we identified the zinc-finger protein B-BOX 7/CONSTANS-LIKE 9 (MdBBX7/MdCOL9), which plays a positive role in apple drought tolerance. The overexpression of MdBBX7 enhanced drought tolerance, whereas knocking down MdBBX7 expression reduced it. Chromatin immunoprecipitation-sequencing (ChIP-seq) analysis identified one cis-element of MdBBX7, CCTTG, as well as its known binding motif, the T/G box. ChIP-seq and RNA-seq identified 1,197 direct targets of MdBBX7, including ETHYLENE RESPONSE FACTOR (ERF1), EARLY RESPONSIVE TO DEHYDRATION 15 (ERD15), and GOLDEN2-LIKE 1 (GLK1) and these were further verified by ChIP-qPCR and electronic mobility shift assays. Yeast two-hybrid screen identified an interacting protein of MdBBX7, RING-type E3 ligase MYB30-INTERACTING E3 LIGASE 1 (MIEL1). Further examination revealed that MdMIEL1 could mediate the ubiquitination and degradation of MdBBX7 by the 26S proteasome pathway. Genetic interaction analysis suggested that MdMIEL1 acts as an upstream factor of MdBBX7. In addition, MdMIEL1 was a negative regulator of the apple drought stress response. Taken together, our results illustrate the molecular mechanisms by which the MdMIEL1–MdBBX7 module influences the response of apple to drought stress.

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Gene involved in the 3q27 translocation associated with B-cell lymphoma, BCL5, encodes a Kruppel-like zinc-finger protein

Blood ◽

10.1182/blood.v83.1.26.26 ◽

1994 ◽

Vol 83 (1) ◽

pp. 26-32 ◽

Cited By ~ 22

Author(s):

T Miki ◽

N Kawamata ◽

S Hirosawa ◽

N Aoki

Keyword(s):

B Cell ◽

Cell Line ◽

Cell Lines ◽

Zinc Finger ◽

Zinc Finger Protein ◽

Cdna Probe ◽

B Cell Lymphoma ◽

Nucleotide Sequencing ◽

Sequencing Analysis

Abstract Chromosomal translocations involving band 3q27 are the recently described nonrandom cytogenetic abnormalities in B-cell malignancies. We have previously cloned the breakpoint region of 3q27, designated as the BCL5 locus, from the B-cell line carrying the t(3;22). The cDNA for the BCL5 gene was cloned from the human liver cDNA library. The nucleotide sequencing analysis showed that the BCL5 gene encodes a potential transcription factor containing six repeats of the Cys2-His2 zinc-finger motif resembling the Drosophila segmentation gene Kruppel. The calculated molecular weight was 78.8 kD, which was supported by an in vitro transcription and translation experiment. A part of the sequence was essentially identical to that of a genomic fragment, ZNF51, previously reported to be located at 3qter. The translocation occurred in the 5′ region of the BCL5 gene, and the protein-coding exons were fused to the Ig-lambda gene in a head-to-head configuration in the cell line carrying t(3;22). The BCL5 cDNA probe detected a major transcript of 3.8 kb in Burkitt's lymphoma cell lines and an aberrant transcript in the t(3;22) cell line, whereas no transcript was detected in myeloid, monocytoid, erythroid, T-lymphoid, and Epstein-Barr virus- immortalized B-lymphoblastoid cell lines.

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Salinity and drought stress effects on foliar ion concentration, water relations, and photosynthetic characteristics of orchard citrus

Australian Journal of Agricultural Research ◽

10.1071/ar9880619 ◽

1988 ◽

Vol 39 (4) ◽

pp. 619 ◽

Cited By ~ 34

Author(s):

JP Syvertsen ◽

J Lloyd ◽

PE Kriedemann

Keyword(s):

Drought Stress ◽

Gas Exchange ◽

Water Potential ◽

Water Relations ◽

Osmotic Potential ◽

High Salinity ◽

Leaf Water ◽

Ion Concentration ◽

Drought Treatment ◽

Mature Leaves

Effects of salinity and drought stress on foliar ion concentration, water relations and net gas exchange were evaluated in mature Valencia orange trees (Citrus sinensis [L.] Osbeck) on Poncirus trifoliata L. Raf. (Tri) or sweet orange (C. sinensis, Swt) rootstocks at Dareton on the Murray River in New South Wales. Trees had been irrigated with river water which averaged 4 mol m-3 chloride (Cl-) or with river water plus NaCl to produce 10, 14 or 20 mol m-3 Cl- during the previous 3 years. Chloride concentrations in leaves of trees on Tri were significantly higher than those on Swt rootstock. Foliar sodium (Na+) and Cl- concentrations increased and potassium (K+) concentrations decreased as leaves aged, especially under irrigation with 20 mol m-3 Cl-. Leaf osmotic potential was reduced as leaves matured and also by high salinity so that reductions in leaf water potential were offset. Mature leaves had a lower stomatal conductances and higher water use efficiency than young leaves. After 2 months of withholding irrigation water, leaves of low salinity trees on Tri rootstock had higher rates of net gas exchange than those on Swt rootstock, indicating rootstock-affected drought tolerance. Previous treatment with 20 mol m-3 Cl- lowered leaf area index of all trees by more than 50%, and resulted in greater reserves of soil moisture under partially defoliated trees after the drought treatment. This was reflected in more rapid evening recovery of leaf water potential and less severe reductions in net gas exchange after drought treatment in high salinity trees on Swt rootstock. High salinity plus drought stress increased Na+ content of leaves on Swt, but not on Tri rootstocks. Drought stress had no additive effect, with high salinity on osmotic potential of mature leaves. Thus, the salinity-induced reduction in leaf area appeared to be independent of the Cl- exclusion capability of the rootstock and decreased the effects of subsequent drought stress on leaf water relations and net gas exchange.

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Response of Selected Garden Roses to Drought Stress

HortScience ◽

10.21273/hortsci.47.8.1050 ◽

2012 ◽

Vol 47 (8) ◽

pp. 1050-1055 ◽

Cited By ~ 8

Author(s):

Xiaoya Cai ◽

Terri Starman ◽

Genhua Niu ◽

Charles Hall ◽

Leonardo Lombardini

Keyword(s):

Drought Stress ◽

Root Growth ◽

Field Capacity ◽

Leaf Water ◽

The Other ◽

Flower Number ◽

Drought Treatment ◽

Greenhouse Study ◽

Drought Tolerant ◽

Substrate Moisture

A greenhouse study was conducted to evaluate the response of four garden roses (Rosa ×hybrid L.), ‘RADrazz’, ‘Belinda’s Dream’, ‘Old Blush’, and ‘Marie Pavie’, to drought stress. Plants grown in containers were subjected to two watering treatments, well-irrigated [water as needed: ≈35% substrate moisture content (SMC) at re-watering] and cyclic drought stress (withholding irrigation until plants exhibit incipient wilting: ≈10% SMC, then re-watering to field capacity for subsequent dry down). Shoot growth and flower number were reduced in the drought treatment compared with the well-irrigated plants in all cultivars with least reduction in ‘RADrazz’. Drought stress reduced root growth in ‘Belinda’s Dream’ and ‘Marie Pavie’, whereas there was no difference in root growth in ‘RADrazz’ and ‘Old Blush’. Decreased SMC induced reduction in net photosynthetic rate (Pn), stomatal conductance (gS), transpiration rate (E), and midday leaf water potential (ψ). Leaf water use efficiency (WUE) increased as SMC decreased in all cultivars. However, the relationship between these physiological parameters and SMC differed among the cultivars. At SMC between 10% and 20%, ‘RADrazz’ had higher Pn, gS, E, and WUE compared with the other three cultivars. Therefore, ‘RADrazz’ was the most drought-tolerant during container production among the cultivars investigated. With lower gas exchange rates and greater reduction in flower number at low SMC, ‘Marie Pavie’ was less drought-tolerant compared with the other three cultivars.

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Gene involved in the 3q27 translocation associated with B-cell lymphoma, BCL5, encodes a Kruppel-like zinc-finger protein

Blood ◽

10.1182/blood.v83.1.26.bloodjournal83126 ◽

1994 ◽

Vol 83 (1) ◽

pp. 26-32 ◽

Cited By ~ 92

Author(s):

T Miki ◽

N Kawamata ◽

S Hirosawa ◽

N Aoki

Keyword(s):

B Cell ◽

Cell Line ◽

Cell Lines ◽

Zinc Finger ◽

Zinc Finger Protein ◽

Cdna Probe ◽

B Cell Lymphoma ◽

Nucleotide Sequencing ◽

Sequencing Analysis

Chromosomal translocations involving band 3q27 are the recently described nonrandom cytogenetic abnormalities in B-cell malignancies. We have previously cloned the breakpoint region of 3q27, designated as the BCL5 locus, from the B-cell line carrying the t(3;22). The cDNA for the BCL5 gene was cloned from the human liver cDNA library. The nucleotide sequencing analysis showed that the BCL5 gene encodes a potential transcription factor containing six repeats of the Cys2-His2 zinc-finger motif resembling the Drosophila segmentation gene Kruppel. The calculated molecular weight was 78.8 kD, which was supported by an in vitro transcription and translation experiment. A part of the sequence was essentially identical to that of a genomic fragment, ZNF51, previously reported to be located at 3qter. The translocation occurred in the 5′ region of the BCL5 gene, and the protein-coding exons were fused to the Ig-lambda gene in a head-to-head configuration in the cell line carrying t(3;22). The BCL5 cDNA probe detected a major transcript of 3.8 kb in Burkitt's lymphoma cell lines and an aberrant transcript in the t(3;22) cell line, whereas no transcript was detected in myeloid, monocytoid, erythroid, T-lymphoid, and Epstein-Barr virus- immortalized B-lymphoblastoid cell lines.

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Drought Stress Triggers Shifts in the Root Microbial Community and Alters Functional Categories in the Microbial Gene Pool

Frontiers in Microbiology ◽

10.3389/fmicb.2021.744897 ◽

2021 ◽

Vol 12 ◽

Author(s):

Jianbo Xie ◽

Ghada E. Dawwam ◽

Amira E. Sehim ◽

Xian Li ◽

Jiadong Wu ◽

...

Keyword(s):

Microbial Community ◽

Drought Stress ◽

Plant Growth ◽

Crop Productivity ◽

Plant Tolerance ◽

Functional Categories ◽

Crop Failure ◽

Drought Treatment ◽

The Impact ◽

Root Microbiome

Drought is a major threat to crop productivity and causes decreased plant growth, poor yields, and crop failure. Nevertheless, the frequency of droughts is expected to increase in the coming decades. The microbial communities associated with crop plants can influence how plants respond to various stresses; hence, microbiome manipulation is fast becoming an effective strategy for improving the stress tolerance of plants. The effect of drought stress on the root microbiome of perennial woody plants is currently poorly understood. Using Populus trees as a model ecosystem, we found that the diversity of the root microbial community decreased during drought treatment and that compositional shifts in microbes during drought stress were driven by the relative abundances of a large number of dominant phyla, including Actinobacteria, Firmicutes, and Proteobacteria. A subset of microbes, including Streptomyces rochei, Bacillus arbutinivorans, B. endophyticus, B. megaterium, Aspergillus terreus, Penicillium raperi, Trichoderma ghanense, Gongronella butleri, and Rhizopus stolonifer, was isolated from the drought-treated poplar rhizosphere soils, which have potentially beneficial to plant fitness. Further controlled inoculation experiments showed that the isolated bacterial and fungal isolates positively impacted plant growth and drought tolerance. Collectively, our results demonstrate the impact of drought on root microbiome structure and provide a novel example of manipulating root microbiomes to improve plant tolerance.

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Mycobacterial and HIV Infections Up-Regulated Human Zinc Finger Protein 134, a Novel Positive Regulator of HIV-1 LTR Activity and Viral Propagation

PLoS ONE ◽

10.1371/journal.pone.0104908 ◽

2014 ◽

Vol 9 (8) ◽

pp. e104908 ◽

Cited By ~ 3

Author(s):

Ronald Benjamin ◽

Atoshi Banerjee ◽

Kannan Balakrishnan ◽

Ramya Sivangala ◽

Sumanlatha Gaddam ◽

...

Keyword(s):

Zinc Finger ◽

Zinc Finger Protein ◽

Hiv Infections ◽

Viral Propagation ◽

Positive Regulator ◽

Finger Protein ◽

Hiv 1

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The Barley Stripe Mosaic Virus expression system reveals the wheat C2H2 zinc finger protein TaZFP1B as a key regulator of drought tolerance

10.21203/rs.2.16980/v1 ◽

2019 ◽

Author(s):

Mario Houde ◽

Arnaud Cheuk ◽

François Ouellet

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Mosaic Virus ◽

Zinc Finger ◽

Zinc Finger Protein ◽

Expression System ◽

Functional Characterization ◽

Barley Stripe Mosaic Virus ◽

Finger Protein

Abstract Drought stress is one of the major factors limiting wheat production globally. Improving drought tolerance is important for agriculture sustainability. Although various morphological, physiological and biochemical responses associated with drought tolerance have been documented, the molecular mechanisms and regulatory genes that are needed to improve drought tolerance in crops require further investigation. We have used a novel 4-component version (for overexpression) and a 3-component version (for underexpression) of a barley stripe mosaic virus-based (BSMV) system for functional characterization of the C2H2-type zinc finger protein TaZFP1B in wheat. These expression systems avoid the need to produce transgenic plant lines and greatly speeds up functional gene characterization.Results We show that overexpression of TaZFP1B stimulates plant growth and up-regulates different oxidative stress-responsive genes under well-watered conditions. Plants that overexpress TaZFP1B are more drought tolerant at critical periods of the plant’s life cycle. Furthermore, RNA-Seq analysis revealed that plants overexpressing TaZFP1B reprogram their transcriptome, resulting in physiological and physical modifications that help wheat to grow and survive under drought stress. In contrast, plants transformed to underexpress TaZFP1B are significantly less tolerant to drought and growth is negatively affected.Conclusions This study clearly shows that the two versions of the BSMV system can be used for fast and efficient functional characterization of genes in crops. The extent of transcriptome reprogramming in plants that overexpress TaZFP1B indicates that the encoded transcription factor is a key regulator of drought tolerance in wheat.

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Expression and analysis of zinc finger family gene in Lenzites gibbosa

Journal of Forestry Research ◽

10.1007/s11676-019-01044-2 ◽

2019 ◽

Vol 31 (5) ◽

pp. 1889-1898

Author(s):

Jun Zhang ◽

Yujie Chi ◽

Shuxuan Li ◽

Jian Zhang ◽

Jie Chen

Keyword(s):

Endoplasmic Reticulum ◽

Differentially Expressed Genes ◽

Zinc Finger ◽

Growth And Development ◽

Zinc Finger Protein ◽

Enrichment Analysis ◽

Protein Processing ◽

Differentially Expressed ◽

Pathway Enrichment Analysis ◽

Go Annotation

Abstract Zinc finger transcription factors play significant roles in the growth and development of plant and animal, but their function remains obscure in fungi. Lenzites gibbosa mycelia were extracted and sequenced by transcriptome analysis after growing on sawdust at different times to support mycelial growth of L. gibbosa in a nutrient matrix. Data bases used for analysis were the Kyoto encyclopedia of genes and genomes (KEGG) annotation, the cluster of orthologous groups of proteins (COG) and gene ontology (GO) annotation. Zinc finger class genes related to the growth and development of L. gibbosa were screened. GO annotation and enrichment analysis of differentially expressed genes were carried out. A total of 114.55 Gb Clean Data were obtained from the L. gibbosa transcriptome. The average Clean Data in each sample was 6.16 Gb. The relative efficiency of reads between each sample and the reference genome was 88.5% to 91.4%. The COG analysis showed that most zinc finger protein genes were related to replication, recombination and repair function. GO enrichment analysis showed that the expressed genes involved in cellular process, cell part and binding. We identified seventy-two expressed genes including seven up-regulated genes and sixty-five down-regulated genes by applying DESeq2 data analysis software. By comparing the significantly expressed genes with KEGG database, 66 annotated sequences were obtained, and 35 primary metabolic pathways were annotated. Pathway enrichment analysis showed that differentially expressed genes were significantly enriched in protein processing in endoplasmic reticulum and ubiquitin-mediated proteolysis pathways. Gene_11750 and gene_5266 are highly correlated with the growth and development of L. gibbosa and are closely related to protein processing in endoplasmic reticulum and ubiquitin-mediated proteolysis pathway. According to gene functional analysis, seven important differentially expressed genes related to the growth and development of L. gibbosa were identified.

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