scholarly journals Patagonin-CRISP: Antimicrobial Activity and Source of Antimicrobial Molecules in Duvernoy’s Gland Secretion (Philodryas patagoniensis Snake)

2021 ◽  
Vol 11 ◽  
Author(s):  
Juliana Cuoco Badari ◽  
Andrea Díaz-Roa ◽  
Marisa Maria Teixeira Rocha ◽  
Ronaldo Zucatelli Mendonça ◽  
Pedro Ismael da Silva Junior
Keyword(s):  
Antimicrobial Activity ◽  
Biological Activity ◽  
Disulfide Bonds ◽  
High Performance ◽  
Amino Acid Sequences ◽  
Low Concentrations ◽  
Family Protein ◽  
Microbial Strains ◽  
First Time ◽  
Homologous Amino Acid

Snake venom contains a variety of toxins with a range of biological activity, among these toxins cysteine-rich secreted proteins (CRISPs) can be found. The proteins of this family have masses of 20–30 kDa and display homologous amino acid sequences containing 16 cysteine residues, forming eight disulfide bonds. Some of these proteins have been explored, characterized, and described in terms of their activity; however, little is known about their range of activities. A search for new antimicrobial molecules is ongoing, as the number of microbial strains resistant to available antibiotics is increasing. We identified antimicrobial activity in the secretion of Duvernoy's gland of the rear-fanged Philodryas patagoniensis. Fractions of this venom were subjected to reverse-phase high performance liquid chromatography and analyzed to determine their antimicrobial activity with a liquid broth inhibition assay. One of the fractions presented activity against a Gram-negative bacterium and a filamentous fungus. This fraction was analyzed with LC-MS/MS, and a protein of 24,848.8 Da was identified. Database searches allowed us to identify it as a CRISP due to the presence of some unique fragments in the molecule. We called it patagonin-CRISP, as the same protein in the venom of P. patagoniensis had previously been characterized as having a different biological activity. Patagonin-CRISP presented activity at very low concentrations and showed no cytotoxic activity. This is the first time that antimicrobial activity has been identified for P. patagoniensis venom or for a CRISP family protein.

scholarly journals BIOLOGICAL ACTIVITY OF ANTIMICROBIAL PEPTIDES FROM CHICKENS THROMBOCYTES

2016 ◽  
pp. 24-29
Author(s):  
M. V. Sycheva ◽  
A. S. Vasilchenko ◽  
E. A. Rogozhin ◽  
T. M. Pashkova ◽  
L. P. Popova ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Peptides ◽  
High Performance ◽  
Bactericidal Effect ◽  
Reverse Phase ◽  
Chromatography Method ◽  
E Coli ◽  
Liquid Chromatography Method ◽  
First Time ◽  
Peptide Fractions

Aim. Isolation and study ofbiological activity of antimicrobial peptides from chickens thrombocytes. Materials and methods. Peptides from chickens thrombocytes, obtained by reverse-phase high-performance liquid chromatography method with stepped and linear gradients of concentration increase of the organic solvent were used in the study. Their antimicrobial activity was determined by microtitration method in broth; mechanism of biological effect - by using fluorescent spectroscopy method with DNA-tropic dyes. Results. Individual fractions of peptides were isolated from chickens thrombocytes, that possess antimicrobial activity against Staphylococcus aureus P209 and Escherichia coli K12. A disruption of integrity of barrier structures of microorganisms under the effect of thrombocyte antimicrobial peptides and predominance of cells with damaged membrane in the population of E. coli was established. Conclusion. The data obtained on antimicrobial activity and mechanism of bactericidal effect of the peptide fractions from chickens thrombocytes isolated for the first time expand the understanding of functional properties of chickens thrombocytes and open a perspective for their further study with the aim of use as antimicrobial means.

scholarly journals A Pair of Novel Sulfonyl-Containing N-Acetyldopamine Dimeric Enantiomers From Aspongopus chinensis

2020 ◽  
Vol 15 (3) ◽  
pp. 1934578X2091127
Author(s):  
Li Liao ◽  
Yong-Ming Yan ◽  
Te Xu ◽  
Hou-Lin Xia ◽  
Yong-Xian Cheng
Keyword(s):  
High Performance Liquid Chromatography ◽  
Biological Activity ◽  
Liquid Chromatography ◽  
Renal Fibrosis ◽  
High Performance ◽  
Racemic Mixture ◽  
Spectroscopic Methods ◽  
Liquid Chromatography Separation ◽  
New Compounds ◽  
First Time

A pair of novel sulfonyl-containing N-acetyldopamine dimer enanitomers, (±)-aspongamide E (1), a new ester 2-aminoethyl ( E)-hex-2-enoate (2), along with 3 known compounds (3-5) were isolated from Aspongopus chinensis. Their structures were determined by spectroscopic methods. Compound 1 is a racemic mixture, chiral high-performance liquid chromatography separation followed by electronic circular dichroism calculations assigned the absolute configurations of 2 enantiomers of 1. Compounds 3-5 were isolated from A. chinensis for the first time. The biological activity of the selected new compounds against renal fibrosis was evaluated.

scholarly journals Synthesis, Antimicrobial Properties, and Inhibition of Catalase Activity of 1,4-Naphtho- and Benzoquinone Derivatives Containing N-, S-, O-Substituted

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Semih Kurban ◽  
Nahide Gulsah Deniz ◽  
Cigdem Sayil ◽  
Mustafa Ozyurek ◽  
Kubilay Guclu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Catalase Activity ◽  
Inhibitory Concentration ◽  
Antimicrobial Properties ◽  
Significant Inhibition ◽  
Inhibition Activity ◽  
Low Concentrations ◽  
Test Culture ◽  
First Time

A series of new 1,4-naphtho- and benzoquinone derivatives possessing N-, S-, O-substituted groups which has not been reported yet has been synthesized from 2,3-dichloro-1,4-naphthoquinone 1 and 2,3,5,6-tetrachlorocyclohexa-2,5-diene-1,4-dione 15 involving a Michael addition. In the synthesized compounds, antimicrobial activity at low concentrations against Escherichia coli B-906, Staphylococcus aureus 209-P, and Mycobacterium luteum B-917 bacteria and Candida tenuis VKM Y-70 and Aspergillus niger F-1119 fungi in comparison with controls was identified. 2-Chloro-3-((2-(piperidin-1-yl)ethyl)amino)naphthalene-1,4-dione 3g and 2,5-dichloro-3-ethoxy-6-((2,4,6-trifluorophenyl)amino)cyclohexa-2,5-diene-1,4-dione 17 were the most potent, with a minimum inhibitory concentration value of 15.6 μg/mL against test-culture M. luteum and S. aureus, respectively. Furthermore, in this work, a catalase activity of benzo- and naphthoquinone derivatives was examined for the first time. The catalase activity of benzo- and naphthoquinone derivatives was determined, showing that compound 3g had significant inhibition activity for catalase enzyme.

scholarly journals Photocatalytic removal of fluoroquinolones and their antimicrobial activity from water matrices at trace levels: a comparison of commercial TiO2 catalysts

2018 ◽  
Vol 78 (8) ◽  
pp. 1668-1678 ◽  
Author(s):  
Wilson Augusto Lima Venancio ◽  
Caio Rodrigues-Silva ◽  
Milena Guedes Maniero ◽  
José Roberto Guimarães
Keyword(s):  
Antimicrobial Activity ◽  
Biological Activity ◽  
High Performance ◽  
Photocatalytic Oxidation ◽  
Antimicrobial Agents ◽  
Solid Phase ◽  
Tap Water ◽  
Uva Irradiation ◽  
The Matrix ◽  
Resultant Solution

Abstract In this study, a solution containing the fluoroquinolones (FQs) ciprofloxacin, lomefloxacin, and ofloxacin (antimicrobial agents) was subjected to photocatalytic oxidation under UVA irradiation, employing the commercial titanium dioxide as catalyst. On-line solid phase extraction coupled to ultra-high-performance liquid chromatography–mass spectroscopy was used to pre-concentrate and quantify the analytes. The process provided an almost 95% degradation efficiency for all the FQs. The TiO2 PC500 (100% anatase) was more efficient than TiO2 P25 (80% anatase) for FQs degradation. The matrix effect on the efficiency of the process was evaluated by ultrapure water – UW, simulated water – SW, bottled water –BW, and public drinking tap water – TW. Simulated water showed lower interference, compared to drinking water and bottled mineral water, due to the lower concentrations of hydroxyl radical scavengers. The assessment of the residual antimicrobial activity in the solution, when using 50 mg L−1 PC500 or 100 mg L−1 P25, showed reductions of biological activity (after 120 min of reaction) of 92.4% and 95.4% for Escherichia coli, and 78.1% and 84.2% for Bacillus subtilis, respectively. It shows that the photocatalytic oxidation process was able to not only degrade the FQs but also deactivate its biological activity in the resultant solution.

scholarly journals Application of EN 16615 (4-Field Test) for the Evaluation of the Antimicrobial Activity of the Selected Commercial and Self-Made Disinfectant Wipes

2021 ◽  
Vol 18 (11) ◽  
pp. 5932
Author(s):  
Stefan Tyski ◽  
Wanda Grzybowska ◽  
Ewa Bocian
Keyword(s):  
Antimicrobial Activity ◽  
Field Test ◽  
Sodium Hypochlorite ◽  
Contact Time ◽  
Multidrug Resistant ◽  
Log10 Reduction ◽  
Disinfection Process ◽  
Microbial Strains ◽  
First Time ◽  
Ammonium Compounds

The purpose of disinfectants is to reduce microorganisms on a contaminated surface and to prevent the spread of microorganisms. The relatively new EN 16615 simulates disinfection by wiping and allows for assessing the recovery of microorganisms from the surface and, importantly, the degree of spread of microorganisms when the surface is disinfected by wiping. For the first time, using this standard, the tested products in the form of commercial disinfectant wipes were compared with self-made wipes soaked in respective disinfectant liquids. The disinfected surfaces were simulated by homogeneous polyvinyl chloride plates. The studies were carried out not only with the standard, but also with clinical multidrug-resistant microbial strains. Based on the research, it can be concluded that the most effective products in the disinfection process (log10 reduction of ≥5) with the shortest contact time (1 min) were products containing ethanol, propanol, and quaternary ammonium compounds (self-made wipes) and propanol (commercial wipes). The least effective products (log10 reduction of <5) in terms of the contact time declared by the manufacturer were products containing ethanol and sodium hypochlorite (commercial wipes). Much better antimicrobial activity of self-made wipes was observed in comparison to the activity of the commercial wipes.

Synthesis of Some New Poly cyclic Heterocyclic Compounds of Anticipated Biological Activity

2020 ◽  
Vol 17 ◽  
Author(s):  
Taghreed A. Alsufyani ◽  
Hosam A. Saad ◽  
Asmaa A. A. Al-otaibi
Keyword(s):  
Antimicrobial Activity ◽  
Biological Activity ◽  
Spectral Data ◽  
Elemental Analysis ◽  
Heterocyclic Compounds ◽  
Biologically Active ◽  
Supplementary Information ◽  
Diffusion Method ◽  
First Time ◽  
Agar Well Diffusion Method

: Novel polyheterocyclic nitrogen systems such as 2-amino-1H-pyrazolo[5',1':3,4][1,2,4]-triazino[5,6-b]indole-3-carbonitrile 2, which has been synthesized for the first time in the present work, is considered as starting material for the synthesis of more novel polyheterocyclic systems with five fused rings and sometime eight fused rings via heterocyclization with α,β-bifunctional reagents under different conditions. The formed structures of the products were established from their correct elemental analysis and spectral data. Some of the compounds were evaluated for their antimicrobial activity using the Agar Well Diffusion method. Compounds 2, 4, 5, 6, 8 were found to be biologically active (Supplementary information).

scholarly journals Antimicrobial Activity of MgB2 Powders Produced via Reactive Liquid Infiltration Method

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4966
Author(s):  
Santanu Kumar Padhi ◽  
Nicoletta Baglieri ◽  
Valentina Bonino ◽  
Angelo Agostino ◽  
Lorenza Operti ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Liquid Infiltration ◽  
Reactive Liquid ◽  
Planktonic Form ◽  
Microbial Strains ◽  
First Time ◽  
Infiltration Method ◽  
Different Levels

We report for the first time on the antimicrobial activity of MgB2 powders produced via the Reactive Liquid Infiltration (RLI) process. Samples with MgB2 wt.% ranging from 2% to 99% were obtained and characterized, observing different levels of grain aggregation and of impurity phases. Their antimicrobial activity was tested against Staphylococcus aureus ATCC BAA 1026, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Candida albicans ATCC 10231. A general correlation is observed between the antibacterial activity and the MgB2 wt.%, but the sample microstructure also appears to be very important. RLI-MgB2 powders show better performances compared to commercial powders against microbial strains in the planktonic form, and their activity against biofilms is also very similar.

scholarly journals Rosemary Essential Oils as a Promising Source of Bioactive Compounds: Chemical Composition, Thermal Properties, Biological Activity, and Gastronomical Perspectives

Foods ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2734
Author(s):  
Darko Micić ◽  
Saša Đurović ◽  
Pavel Riabov ◽  
Ana Tomić ◽  
Olja Šovljanski ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Biological Activity ◽  
Thermal Properties ◽  
Chemical Composition ◽  
Essential Oils ◽  
Sunflower Oils ◽  
Microbial Strains ◽  
Antioxidant Agent ◽  
Rosmarinus Officinalis L ◽  
Promising Source

Rosemary (Rosmarinus officinalis L.) is a plant worldwide cultivated mainly for essential oils, extracts, and as a spice. Up-to-date results showed diversity in composition of the essential oils, which may influence their quality, biological activity, and thermal properties. Therefore, the aim of this study was to investigate the chemical composition, antimicrobial activity, and thermal properties of the rosemary essential oils originating from Serbia and Russia. Additionally, oils were added to the sunflower oils in order to investigate possible antioxidant activity during the frying. Investigation of the chemical profile marked α-pinene, eucalyptol, and camphor as the most abundant compounds in both oils. However, overall composition influenced in such manner that Russian oil showed significantly higher antimicrobial activity, while Serbian oil proved to be better antioxidant agent in case of frying of sunflower oil. This would significantly influence possible application of the oils, which could be used as an antioxidant agent for extension of the food shelf life, or antimicrobial agent for protection against different microbial strains.

Antimicrobial Activity of 2-((2-(hydroxyimino)-1- phenylpropylidene) Hydrazono)-1, 2- diphenylethanone (HMPPH) and its Derivative

2020 ◽  
Vol 5 (7) ◽  
pp. 1056-1060
Author(s):  
M.J. Lele ◽  
Dr.R.G. Deshmukh
Keyword(s):  
Antimicrobial Activity ◽  
Biological Activity ◽  
Schiff Base ◽  
Elemental Analysis ◽  
Magnetic Moment ◽  
Electronic Spectra ◽  
Spectroscopic Investigation ◽  
Molecular Formula ◽  
Preliminary Screening ◽  
First Time

A new series of Cu(II), Pt(II) and Pd(II) complexes were prepared with ligand 2-((2- (hydroxyimino)-1-phenylpropylidene) Hydrazono)-1, 2- diphenylethanone (HMPPH) corresponding to molecular formula (C23H18N3O2) has been synthesized and reported for the first time. The Schiff base and its metal complexes were characterized on the basis of various spectroscopic investigation like IR, NMR, UVVISIBLE spectroscopy, elemental analysis etc. While the geometry of the complexes was confirmed by electronic spectra, magnetic moment measurements. A preliminary screening of these compounds for biological activity against various microorganisms has indicated that they are microbial active against some microorganisms.

Superionic Lithium Intercalation through 2 nm x 2 nm Columns in the Crystallographic Shear Phase Nb18W8O69

2019 ◽  
Author(s):  
Kent Griffith ◽  
Clare Grey
Keyword(s):  
High Performance ◽  
Rate Capability ◽  
Block Size ◽  
Lithium Intercalation ◽  
Resonance Spectroscopy ◽  
Complex Metal Oxides ◽  
Battery Electrode ◽  
Crystallographic Shear ◽  
Battery Material ◽  
First Time

Nb18W8O69 (9Nb2O5×8WO3) is the tungsten-rich end-member of the Wadsley–Roth crystallographic shear (cs) structures within the Nb2O5–WO3 series. It has the largest block size of any known, stable Wadsley–Roth phase, comprising 5 ´ 5 units of corner-shared MO6 octahedra between the shear planes, giving rise to 2 nm ´ 2 nm blocks. Rapid lithium intercalation is observed in this new candidate battery material and 7Li pulsed field gradient nuclear magnetic resonance spectroscopy – measured in a battery electrode for the first time at room temperature – reveals superionic lithium conductivity. In addition to its promising rate capability, Nb18W8O69 adds a piece to the larger picture of our understanding of high-performance Wadsley–Roth complex metal oxides.

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