Proline accumulation and metabolism-related genes expression profiles in Kosteletzkya virginica seedlings under salt stress

Salt tolerance is an important trait in soybean cultivation and breeding. Plant responses to salt stress include physiological and biochemical changes that affect the movement of water across the plasma membrane. Plasma membrane intrinsic proteins (PIPs) localize to the plasma membrane and regulate the water and solutes flow. In this study, quantitative real-time PCR and yeast two-hybridization were engaged to analyze the early gene expression profiles and interactions of a set of soybean PIPs (GmPIPs) in response to salt stress. A total of 20 GmPIPs-encoding genes had varied expression profiles after salt stress. Among them, 13 genes exhibited a downregulated expression pattern, including GmPIP1;6, the constitutive overexpression of which could improve soybean salt tolerance, and its close homologs GmPIP1;7 and 1;5. Three genes showed upregulated patterns, including the GmPIP1;6 close homolog GmPIP1;4, when four genes with earlier increased and then decreased expression patterns. GmPIP1;5 and GmPIP1;6 could both physically interact strongly with GmPIP2;2, GmPIP2;4, GmPIP2;6, GmPIP2;8, GmPIP2;9, GmPIP2;11, and GmPIP2;13. Definite interactions between GmPIP1;6 and GmPIP1;7 were detected and GmPIP2;9 performed homo-interaction. The interactions of GmPIP1;5 with GmPIP2;11 and 2;13, GmPIP1;6 with GmPIP2;9, 2;11 and GmPIP2;13, and GmPIP2;9 with itself were strengthened upon salt stress rather than osmotic stress. Taken together, we inferred that GmPIP1 type and GmPIP2 type could associate with each other to synergistically function in the plant cell; a salt-stress environment could promote part of their interactions. This result provided new clues to further understand the soybean PIP–isoform interactions, which lead to potentially functional homo- and heterotetramers for salt tolerance.

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Gene expression of halophyte Kosteletzkya virginica seedlings under salt stress at early stage

Genetica ◽

10.1007/s10709-009-9384-9 ◽

2009 ◽

Vol 137 (2) ◽

pp. 189-199 ◽

Cited By ~ 11

Author(s):

Yu-Qi Guo ◽

Zeng-Yuan Tian ◽

Guang-Yong Qin ◽

Dao-Liang Yan ◽

Jie Zhang ◽

...

Keyword(s):

Gene Expression ◽

Salt Stress ◽

Early Stage ◽

Kosteletzkya Virginica

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Proline accumulation, ion homeostasis and antioxidant defence system alleviate salt stress and protect carbon assimilation in bread wheat genotypes of Omani origin

Environmental and Experimental Botany ◽

10.1016/j.envexpbot.2021.104687 ◽

2021 ◽

pp. 104687

Author(s):

Marwa Sulaiman Al Hinai ◽

Aman Ullah ◽

Rashid Said Al-Rajhi ◽

Muhammad Farooq

Keyword(s):

Salt Stress ◽

Bread Wheat ◽

Ion Homeostasis ◽

Carbon Assimilation ◽

Proline Accumulation ◽

Antioxidant Defence ◽

Wheat Genotypes ◽

Defence System ◽

Antioxidant Defence System

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The Study of SOS Genes Expression in Mutant Barley Root under Salt Stress

Journal of Crop Breeding ◽

10.29252/jcb.11.29.1 ◽

2019 ◽

Vol 11 (29) ◽

pp. 1-8

Author(s):

Sareh Yousefi Rad ◽

◽

Hassan Soltanloo ◽

Sayad Sanaz Ramezanpour ◽

Khalil Zaynali Nezhad ◽

...

Keyword(s):

Salt Stress ◽

Barley Root ◽

Genes Expression

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Genome-wide identification and expression profiles of ERF subfamily transcription factors in Zea mays

PeerJ ◽

10.7717/peerj.9551 ◽

2020 ◽

Vol 8 ◽

pp. e9551

Author(s):

Lidong Hao ◽

Shubing Shi ◽

Haibin Guo ◽

Ming Li ◽

Pan Hu ◽

...

Keyword(s):

Transcription Factors ◽

Plant Growth ◽

Growth And Development ◽

Expression Profiles ◽

Vital Role ◽

Ethylene Response Factor ◽

Plant Growth And Development ◽

Element Analysis ◽

Genome Wide ◽

Genes Expression

The Ethylene-Response Factor (ERF) subfamily transcription factors (TFs) belong to the APETALA2/Ethylene-Responsive Factor (AP2/ERF) superfamily and play a vital role in plant growth and development. However, identification and analysis of the ERF subfamily genes in maize have not yet been performed at genome-wide level. In this study, a total of 76 ERF subfamily TFs were identified and were found to be unevenly distributed on the maize chromosomes. These maize ERF (ZmERF) TFs were classified into six groups, namely groups B1 to B6, based on phylogenetic analysis. Synteny analysis showed that 50, 54, and 58 of the ZmERF genes were orthologous to those in rice, Brachypodium, and Sorghum, respectively. Cis-element analysis showed that elements related to plant growth and development, hormones, and abiotic stress were identified in the promoter region of ZmERF genes. Expression profiles suggested that ZmERF genes might participate in plant development and in response to salinity and drought stresses. Our findings lay a foundation and provide clues for understanding the biological functions of ERF TFs in maize.

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Genome-Wide Analysis of the TCP Gene Family in Switchgrass (Panicum virgatum L.)

International Journal of Genomics ◽

10.1155/2019/8514928 ◽

2019 ◽

Vol 2019 ◽

pp. 1-13 ◽

Cited By ~ 2

Author(s):

Yuzhu Huo ◽

Wangdan Xiong ◽

Kunlong Su ◽

Yu Li ◽

Yawen Yang ◽

...

Keyword(s):

Salt Stress ◽

Plant Growth ◽

Gene Family ◽

Stress Responses ◽

Panicum Virgatum ◽

Expression Profiles ◽

Specific Expression ◽

Genome Wide Analysis ◽

Genome Wide ◽

A Genome

The plant-specific transcription factor TCPs play multiple roles in plant growth, development, and stress responses. However, a genome-wide analysis of TCP proteins and their roles in salt stress has not been declared in switchgrass (Panicum virgatum L.). In this study, 42 PvTCP genes (PvTCPs) were identified from the switchgrass genome and 38 members can be anchored to its chromosomes unevenly. Nine PvTCPs were predicted to be microRNA319 (miR319) targets. Furthermore, PvTCPs can be divided into three clades according to the phylogeny and conserved domains. Members in the same clade have the similar gene structure and motif localization. Although all PvTCPs were expressed in tested tissues, their expression profiles were different under normal condition. The specific expression may indicate their different roles in plant growth and development. In addition, approximately 20 cis-acting elements were detected in the promoters of PvTCPs, and 40% were related to stress response. Moreover, the expression profiles of PvTCPs under salt stress were also analyzed and 29 PvTCPs were regulated after NaCl treatment. Taken together, the PvTCP gene family was analyzed at a genome-wide level and their possible functions in salt stress, which lay the basis for further functional analysis of PvTCPs in switchgrass.

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LncExpDB: an expression database of human long non-coding RNAs

Nucleic Acids Research ◽

10.1093/nar/gkaa850 ◽

2020 ◽

Vol 49 (D1) ◽

pp. D962-D968 ◽

Cited By ~ 2

Author(s):

Zhao Li ◽

Lin Liu ◽

Shuai Jiang ◽

Qianpeng Li ◽

Changrui Feng ◽

...

Keyword(s):

Expression Profiles ◽

Biological Functions ◽

Protein Coding ◽

Web Interfaces ◽

Functional Studies ◽

Protein Coding Genes ◽

Genes Expression ◽

Wide Range ◽

Non Coding Rnas ◽

User Friendly

Abstract Expression profiles of long non-coding RNAs (lncRNAs) across diverse biological conditions provide significant insights into their biological functions, interacting targets as well as transcriptional reliability. However, there lacks a comprehensive resource that systematically characterizes the expression landscape of human lncRNAs by integrating their expression profiles across a wide range of biological conditions. Here, we present LncExpDB (https://bigd.big.ac.cn/lncexpdb), an expression database of human lncRNAs that is devoted to providing comprehensive expression profiles of lncRNA genes, exploring their expression features and capacities, identifying featured genes with potentially important functions, and building interactions with protein-coding genes across various biological contexts/conditions. Based on comprehensive integration and stringent curation, LncExpDB currently houses expression profiles of 101 293 high-quality human lncRNA genes derived from 1977 samples of 337 biological conditions across nine biological contexts. Consequently, LncExpDB estimates lncRNA genes’ expression reliability and capacities, identifies 25 191 featured genes, and further obtains 28 443 865 lncRNA-mRNA interactions. Moreover, user-friendly web interfaces enable interactive visualization of expression profiles across various conditions and easy exploration of featured lncRNAs and their interacting partners in specific contexts. Collectively, LncExpDB features comprehensive integration and curation of lncRNA expression profiles and thus will serve as a fundamental resource for functional studies on human lncRNAs.

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Granulosa-Lutein Cell Sirtuin Gene Expression Profiles Differ between Normal Donors and Infertile Women

International Journal of Molecular Sciences ◽

10.3390/ijms21010295 ◽

2019 ◽

Vol 21 (1) ◽

pp. 295

Author(s):

Rebeca González-Fernández ◽

Rita Martín-Ramírez ◽

Deborah Rotoli ◽

Jairo Hernández ◽

Frederick Naftolin ◽

...

Keyword(s):

Gene Expression ◽

Protein Localization ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Cellular Protein ◽

Control Group ◽

Poor Responders ◽

Male Factor ◽

Expression Levels ◽

Genes Expression

Sirtuins are a family of deacetylases that modify structural proteins, metabolic enzymes, and histones to change cellular protein localization and function. In mammals, there are seven sirtuins involved in processes like oxidative stress or metabolic homeostasis associated with aging, degeneration or cancer. We studied gene expression of sirtuins by qRT-PCR in human mural granulosa-lutein cells (hGL) from IVF patients in different infertility diagnostic groups and in oocyte donors (OD; control group). Study 1: sirtuins genes’ expression levels and correlations with age and IVF parameters in women with no ovarian factor. We found significantly higher expression levels of SIRT1, SIRT2 and SIRT5 in patients ≥40 years old than in OD and in women between 27 and 39 years old with tubal or male factor, and no ovarian factor (NOF). Only SIRT2, SIRT5 and SIRT7 expression correlated with age. Study 2: sirtuin genes’ expression in women poor responders (PR), endometriosis (EM) and polycystic ovarian syndrome. Compared to NOF controls, we found higher SIRT2 gene expression in all diagnostic groups while SIRT3, SIRT5, SIRT6 and SIRT7 expression were higher only in PR. Related to clinical parameters SIRT1, SIRT6 and SIRT7 correlate positively with FSH and LH doses administered in EM patients. The number of mature oocytes retrieved in PR is positively correlated with the expression levels of SIRT3, SIRT4 and SIRT5. These data suggest that cellular physiopathology in PR’s follicle may be associated with cumulative DNA damage, indicating that further studies are necessary.

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Inducing salt tolerance in sweet corn by magnetic priming

Acta agriculturae Slovenica ◽

10.14720/aas.2017.109.1.09 ◽

2017 ◽

Vol 109 (1) ◽

pp. 89 ◽

Cited By ~ 13

Author(s):

Soheil Karimi ◽

Saeid ESHGHI ◽

Saeid KARIMI ◽

Saman HASAN-NEZHADIAN

Keyword(s):

Salt Stress ◽

Seed Germination ◽

Plant Growth ◽

Sweet Corn ◽

Germination Rate ◽

Nacl Stress ◽

Proline Accumulation ◽

Membrane Thermostability ◽

Germination And Growth ◽

Magnetic Priming

This study evaluates seed germination and growth of sweet corn under NaCl stress (0, 50, and 100 mM), after exposing the seeds to weak (15 mT) or strong (150 mT) magnetic fields (MF) for different durations (0, 6, 12, and 24 hours). Salinity reduced seed germination and plant growth. MF treatments enhanced rate and percentage of germination and improved plant growth, regardless of salinity. Higher germination rate was obtained by the stronger MF, however, the seedling were more vigorous after priming with 15 mT MF. Proline accumulation was observed in parallel with the loss of plant water content under 100 mM NaCl stress. MF prevented proline accumulation by improving water absorption. Positive correlation between H2O2 accumulation and membrane thermostability (MTI) was found after MF treatments, which revealed that MF primed the plant for salinity by H2O2 signaling. However, over-accumulation of H2O2 after prolonged MF exposure adversely affected MTI under severe salt stress. In conclusion, magnetic priming for 6 hours was suggested for enhancing germination and growth of sweet corn under salt stress.

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