scholarly journals Overexpression of a Grapevine Sucrose Transporter (VvSUC27) in Tobacco Improves Plant Growth Rate in the Presence of Sucrose In vitro

2017 ◽  
Vol 8 ◽  
Author(s):  
Yumeng Cai ◽  
Wenrui Tu ◽  
Yunyun Zu ◽  
Jing Yan ◽  
Zimo Xu ◽  
...  
Keyword(s):  
Growth Rate ◽  
Plant Growth ◽  
Sucrose Transporter

Production of tumours and roots by Ephedra following Agrobacterium rhizogenes infection

1994 ◽  
Vol 72 (2) ◽  
pp. 203-207 ◽  
Author(s):  
Niamh A. O'Dowd ◽  
David H. S. Richardson
Keyword(s):  
Growth Rate ◽  
Plant Growth ◽  
Agrobacterium Rhizogenes ◽  
Dry Weight ◽  
Wild Type ◽  
Bacterial Strains ◽  
Heat Treated ◽  
Slow Growing

This paper contains the first report that stems of the Gnetophyte Ephedra respond to infection by Agrobacterium rhizogenes by producing roots and tumours in vivo and in vitro. Of the bacterial strains employed, the wild-type Ar2629 gave the maximum response, and strain LBA9402 was also effective. In no case did heat-treated A. rhizogenes produce tumours or roots. Excised tumour tissues were cultured for more than 2 years in the absence of exogenous plant-growth regulators without any deterioration in growth rate. In vivo tumours of Ephedra fragilis and Ephedra minima contained up to 0.3% dry weight l-ephedrine, and slow-growing in vitro cultured tumours of E. fragilis contained up to 0.01% l-ephedrine, but alkaloid was not detected in faster growing isolates. Key words: Agrobacterium rhizogenes, alkaloid, Ephedra, l-ephedrine, Gnetophytes, gymnosperm, tumours.

scholarly journals Analysis the effect of plant growth regulator (Gibgro-20T) on seaweed Eucheuma cottonii by in vitro

2021 ◽  
Vol 5 (1) ◽  
pp. 15-19
Author(s):  
Muhammad Kasnir ◽  
Khairun Nisaa ◽  
Harlina Harlina ◽  
Masyita Masyita
Keyword(s):  
Growth Rate ◽  
Plant Growth ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Length Growth ◽  
Highest Weight ◽  
Weight Growth ◽  
Randomized Design ◽  
Eucheuma Cottonii

This study aims to determine the effect of the dose of plant growth regulator (PGR) Gibgro-20T on the growth of Eucheuma cottonii in-vitro. The experimental method used was a Completely Randomized Design (CRD) treatment with a Gibgro-20T dose of 0 ppm (control), 5 ppm, 10 ppm, and 15 ppm. The results showed that the highest weight growth rate (RGRW) obtained at 10 ppm PGR dose, which is 1.27% per day. Meanwhile, the highest length growth rate (RGRL) obtained at a dose of 10 ppm, which was 1.72% per day, and the highest RGRL was significantly different from the control (0 ppm) and 5 ppm but was not different real (P<0.05) with 15 ppm treatment. Using Gibgro-20T at doses of 5, 10, and 15 ppm, however, doses of 10 and 15 ppm show higher length growth.

Assessing the growth and saponin accumulation in hairy and adventitious roots of Vietnamese ginseng (Panax vietnamensis Ha et Grushv.)

2016 ◽  
Vol 14 (2) ◽  
pp. 231-236
Author(s):  
Trịnh Thị Hương ◽  
Phạm Bích Ngọc ◽  
Chu Hoàng Hà ◽  
Dương Tấn Nhựt
Keyword(s):  
Growth Rate ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Hairy Roots ◽  
Growth Regulators ◽  
Adventitious Roots ◽  
Dry Matter ◽  
Ginseng Root ◽  
Free Medium

In this study, adventitious and hairy roots of Vietnamese ginseng were used to assess the ability of growth and saponin accumulation. Adventitious roots were derived from leaf samples in vitro (1.0 x 1.0 cm of size) cultured on SH medium supplemented with 5.0 mg.l-1 IBA, 30 g.l-1 sucrose, 8 g.l-1 agar, pH 5.8, and subcultured on the same medium for multiplication. Hairy roots were derived from callus infected with Agrobacterium rhizogenes strain ATCC 15834, then these roots were cultured on plant growth regulators-free SH medium supplemented with 50 g.l-1 sucrose, 8 g.l-1 agar, pH 5.8. During the early culture of two months, the results showed that the growth rate of hairy roots was lower than that of adventitious roots. However, in the later period of culture, the growth rate of hairy roots was higher than that of adventitious roots. After 5 months of culture, the growth rate of hairy roots was 20.87 times and they kept growing as well as branching, while the growth rate of adventitious roots was only 13.52 times and they did not grow further after three months of culture. Analytical results showed that the total saponins of total dry matter of hairy roots (0.101 mg) were higher than that of adventitious roots (0.0681 mg). The main ginsenoside of hairy roots (MR2) was also higher than that of adventitious roots 3.03 fold. In addition, the hairy roots grew on plant growth regulators-free medium while adventitious roots grew on medium supplemented with auxin. Therefore, hairy roots proved to be suitable source material for Vietnamese ginseng root biomass production in the bioreactor systems.

scholarly journals Phytase-Producing Rahnella aquatilis JZ-GX1 Promotes Seed Germination and Growth in Corn (Zea mays L.)

2021 ◽  
Vol 9 (8) ◽  
pp. 1647
Author(s):  
Gui-E Li ◽  
Wei-Liang Kong ◽  
Xiao-Qin Wu ◽  
Shi-Bo Ma
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Biomass Accumulation ◽  
Root Surface ◽  
Phosphorus Concentration ◽  
Maize Seedlings ◽  
Total Phosphorus Concentration ◽  
Rahnella Aquatilis ◽  
Maize Seeds

Phytase plays an important role in crop seed germination and plant growth. In order to fully understand the plant growth-promoting mechanism by Rahnella aquatilis JZ-GX1，the effect of this strain on germination of maize seeds was determined in vitro, and the colonization of maize root by R. aquatilis JZ-GX1 was observed by scanning electron microscope. Different inoculum concentrations and Phytate-related soil properties were applied to investigate the effect of R. aquatilis JZ-GX1 on the growth of maize seedlings. The results showed that R. aquatilis JZ-GX1 could effectively secrete indole acetic acid and had significantly promoted seed germination and root length of maize. A large number of R. aquatilis JZ-GX1 cells colonized on the root surface, root hair and the root interior of maize. When the inoculation concentration was 107 cfu/mL and the insoluble organophosphorus compound phytate existed in the soil, the net photosynthetic rate, chlorophyll content, phytase activity secreted by roots, total phosphorus concentration and biomass accumulation of maize seedlings were the highest. In contrast, no significant effect of inoculation was found when the total P content was low or when inorganic P was sufficient in the soil. R. aquatilis JZ-GX1 promotes the growth of maize directly by secreting IAA and indirectly by secreting phytase. This work provides beneficial information for the development and application of R. aquatilis JZ-GX1 as a microbial fertilizer in the future.

scholarly journals Characterization of Plant Growth-Promoting Traits and Inoculation Effects on Triticum durum of Actinomycetes Isolates under Salt Stress Conditions

Soil Systems ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 26
Author(s):  
Rihab Djebaili ◽  
Marika Pellegrini ◽  
Massimiliano Rossi ◽  
Cinzia Forni ◽  
Maria Smati ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Salt Stress ◽  
Plant Growth ◽  
Durum Wheat ◽  
Growth And Development ◽  
Indole Acetic Acid ◽  
Greenhouse Experiment ◽  
Hydrocyanic Acid ◽  
In Vitro Assays

This study aimed to characterize the halotolerant capability, in vitro, of selected actinomycetes strains and to evaluate their competence in promoting halo stress tolerance in durum wheat in a greenhouse experiment. Fourteen isolates were tested for phosphate solubilization, indole acetic acid, hydrocyanic acid, and ammonia production under different salt concentrations (i.e., 0, 0.25, 0.5, 0.75, 1, 1.25, and 1.5 M NaCl). The presence of 1-aminocyclopropane-1-carboxylate deaminase activity was also investigated. Salinity tolerance was evaluated in durum wheat through plant growth and development parameters: shoot and root length, dry and ash-free dry weight, and the total chlorophyll content, as well as proline accumulation. In vitro assays have shown that the strains can solubilize inorganic phosphate and produce indole acetic acid, hydrocyanic acid, and ammonia under different salt concentrations. Most of the strains (86%) had 1-aminocyclopropane-1-carboxylate deaminase activity, with significant amounts of α-ketobutyric acid. In the greenhouse experiment, inoculation with actinomycetes strains improved the morpho-biochemical parameters of durum wheat plants, which also recorded significantly higher content of chlorophylls and proline than those uninoculated, both under normal and stressed conditions. Our results suggest that inoculation of halotolerant actinomycetes can mitigate the negative effects of salt stress and allow normal growth and development of durum wheat plants.

scholarly journals Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Nisar Ahmad Zahid ◽  
Hawa Z.E. Jaafar ◽  
Mansor Hakiman
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Zingiber Officinale ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Zingiber Officinale Roscoe ◽  
Planting Materials ◽  
Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

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