scholarly journals Combined Effect of Shegandilong Granule and Doxycycline on Immune Responses and Protection Against Avian Infectious Bronchitis Virus in Broilers

2021 ◽  
Vol 8 ◽  
Author(s):  
Haipeng Feng ◽  
Xuezhi Wang ◽  
Jingyan Zhang ◽  
Kang Zhang ◽  
Wenshu Zou ◽  
...  
Keyword(s):  
Drinking Water ◽  
Respiratory Tract ◽  
Immunoglobulin A ◽  
Maximum Level ◽  
Group Iv ◽  
Economic Losses ◽  
Group Iii ◽  
Infectious Bronchitis ◽  
Group I ◽  
Commercial Chicken

Infectious bronchitis (IB) causes significant economic losses to commercial chicken farms due to the failures of vaccine immunization or incomplete protection. In this study, we evaluated the combination effect of Shegandilong (SGDL) granule (a traditional Chinese veterinary medicine) and doxycycline on the prevention of IBV infection and injury in the respiratory tract in broilers. A total of 126, 7-day-old broilers were randomly divided into four groups after vaccination. Group I served as a control. Broilers in Group II were given doxycycline, and Group III was given SGDL granule through drinking water. Broilers in Group IV were given SGDL granule and doxycycline by drinking water. Broilers in all groups were challenged with IBV through intraocular and intranasal routes at day 28. Results showed that the anti-IBV antibody level was higher in group IV compared with the level in other groups. Immunohistochemistry and ELISA results showed that an increase of immunoglobulin A (IgA) was observed in the trachea with the maximum level observed at day 14. In addition, SGDL granule + doxycycline effectively inhibited IBV replication and stopped IBV propagation from the trachea to the lung; modulated the mRNA expressions of IL-1β, IL-6, TNF-α, and IFN-γ; and extenuated the histopathology lesions in trachea and lung. These data imply that a combination of SGDL granule and doxycycline is effective in preventing IBV infection and respiratory tract injury in broilers.

Protective effect of L-carnitine against oxidative damage caused by experimental chronic aflatoxicosis in quail (Coturnix coturnix)

2005 ◽  
Vol 53 (3) ◽  
pp. 319-324 ◽  
Author(s):  
M. Citil ◽  
V. Gunes ◽  
O. Atakisi ◽  
Ayla Ozcan ◽  
M. Tuzcu ◽  
...  
Keyword(s):  
Drinking Water ◽  
Whole Blood ◽  
Reduced Glutathione ◽  
Group Iv ◽  
Control Group ◽  
Total Aflatoxin ◽  
Group Iii ◽  
Coturnix Coturnix ◽  
Group I ◽  
Plasma Malondialdehyde

This study was designed to evaluate the effect of L-carnitine supplementation on the plasma malondialdehyde (MDA) and whole blood reduced glutathione (GSH) concentrations in experimentally-induced chronic aflatoxicosis in quails. For this purpose, a total of 80 quails up to 8 weeks old were divided into four equal groups. Group I served as control, Group II was given L-carnitine at the dose of 200 mg/litre in the drinking water for 60 days, Group III was given 60 µg total aflatoxin/kg diet for 60 days, and Group IV was given both 60 µg total aflatoxin/kg diet and 200 mg L-carnitine/litre in the drinking water for 60 days. Aflatoxin treatment caused a significant increase in plasma MDA and a significant decrease in blood GSH concentrations. On the other hand, there was a significant decrease in plasma MDA and a significant increase in whole blood GSH in the L-carnitine-supplemented group. The present study demonstrated that L-carnitine brought about the inhibition of lipid peroxidation by enhancing antioxidant capacity in quails with chronic aflatoxicosis.

scholarly journals PRENATAL AND POSTNATAL EFFECT OF LEAD ACETATE ON THE HISTOLOGY OF FRONTAL CORTEX AND MOTOR ACTIVITY IN WISTAR RATS

2021 ◽  
pp. 10-12
Author(s):  
SALEH NUHU ◽  
HAUWA IDRIS AHMAD ◽  
AISHA MUHAMMAD GARBA ◽  
TASIU ABDULLAHI SULAIMAN
Keyword(s):  
Drinking Water ◽  
Motor Activity ◽  
Frontal Cortex ◽  
Wistar Rats ◽  
Histological Study ◽  
Group Iv ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Group Ii

Objectives: The objective of this study was to find the histologic and motor activity effect of lead on prenatally and postnatally exposed Wistar rats. Methods: In this study, twelve Wistar Rats were used and grouped into four groups of two females and one male. Group I rats served as the control and allowed feed and water freely. The rats in Group II were administered 500ppm of Pb through drinking water from gestation day 8 (GD8) to parturition (GD21). While Group III rats were given 500ppm of Pb in drinking water from postnatal day 1 (PND1) to PND21. The rats in the fourth group (Group IV) were given 500ppm of Pb from GD8 to PND21. Palmer grasp reflex was conducted to assess the motor activity of the rat pups. The animals were then humanely sacrificed and the frontal cortices were isolated for routine histological processing. Results: The histological study has shown normal neurons in the control group while degenerating cells exhibiting karyolysis, pyknosis, karyorrhexis, vacuolation were seen in the lead-treated groups. Group II and Group IV showed considerate deficit in their motor activity while Group III showed mild effect. Conclusion: From this study, lead exposure of Wistar rats at both prenatal and postnatal period of development has effect on the histology of the frontal cortex as well as on their motor activity.

scholarly journals Ultrastructural evaluation of urine alkalinization versus hydration on colistin-induced nephrotoxicity

2019 ◽  
Vol 38 (12) ◽  
pp. 1366-1377
Author(s):  
B Korucu ◽  
I Unal ◽  
M Pekcan ◽  
AC Inkaya ◽  
H Yeter ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Drinking Water ◽  
Weak Acid ◽  
Group Iv ◽  
Ultrastructural Changes ◽  
Sprague Dawley ◽  
Group Iii ◽  
Group I ◽  
Urine Alkalinization ◽  
Group Ii

Objectives: Colistin is a vital antibiotic used in multidrug-resistant infections. Its most important side effect is nephrotoxicity. Colistin is a weak acid. This study aims to evaluate whether urine alkalinization is protective in the nephrotoxicity of colistin. Methods: Twenty-eight male Sprague-Dawley rats were divided into groups. Group I ( n = 4) was injected with intramuscular distilled water twice a day for 7 days. Group II ( n = 8) was injected with 750,000 IU/kg/day colistin for 7 days. Group III ( n = 8) was injected with the same dose of colistin after their urinary pH was ≥7 through the addition of bicarbonate in their drinking water. Group IV ( n = 8) was injected with the same dose of colistin after their urine density fell below 1010 through the addition of NaCl molds in their food and 12.6 mg/L NaCl in their drinking water. Results: According to tubular degenerations (scored 0–5), group I scored 0, group II scored 4.25, group III scored 2, and group IV scored 1.5. In groups III and IV, protection was achieved ( p = 0.001). The bicarbonate group was not superior to the NaCl group ( p = 0.789). In transmission electron microscopy, group III had more microvilli integrity and autophagic vacuoles compared to group IV. Group IV had mitochondrial swelling and cristae lysis. A lower urine density was related to lower tubular scores ( p = 0.001). Conclusions: Colistin was highly nephrotoxic without protection. Light microscopy findings revealed that urinary alkalinization and NaCl hydration were similarly protective. Urine alkalinization further prevents ultrastructural changes as revealed by electron microscopy.

Analysis of surface conditioning methods on core-veneer bond strength of CAD/CAM zirconia restorations

2020 ◽  
pp. 1-11
Author(s):  
Anip K. Roy ◽  
Govind N. Prasad ◽  
Tushar V. Bhagat ◽  
Saurabh Chaturvedi ◽  
Vishwanath Gurumurthy ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Bond Strength ◽  
Shear Bond Strength ◽  
Group Iv ◽  
Control Group ◽  
Group Iii ◽  
Surface Conditioning ◽  
Group I ◽  
Alumina Particles ◽  
Scanning Electron

BACKGROUND: The increased strength of zirconia has resulted in its widespread application in clinical dentistry. Nevertheless, the fracture of veneering porcelains remains one of the key reasons of failure. OBJECTIVE: The objective of this study was to compare and analyze the influence of surface conditioning methods on the core-veneer bond strength of zirconia restorations. METHODS: Thirty specimens of zirconia core with sizes 10 × 5 × 5 mm were layered with porcelain of sizes 5 × 3 × 3 mm. On the basis of different surface conditioning methods, four groups were made: Group I: abrasion with airborne alumina particles of 110 μm size, Group II: sandblasting with silica coated alumina particles of 50 μm in size, Group III (modified group): alteration with a coating of zirconia powder prior to sintering, and Group IV (control group): metal core specimens. The shear force of all specimens was tested using a universal testing machine with a 0.5 mm/min crosshead speed. One-way analysis of variance (ANOVA) and Tukey’s post hoc pair wise comparison (p= 0.05) were performed to analyze the shear bond strength. A scanning electron microscope was used to assess the fractured specimens. RESULTS: A statistically significant difference was noted between the groups. The mean value of shear bond strength was 40.25 MPa for Group I, 41.93 MPa for Group II, 48.08 MPa for Group III and 47.01 MPa for Group IV. CONCLUSIONS: The modified zirconia group and control group demonstrated a significantly higher mean bond strength than that of Group I, where airborne particle abrasion was used. The scanning electron microscope showed that cohesive fracture in the porcelain veneers was the main problem of failure in altered zirconia. The modified zirconia specimens in Group III demonstrated significantly improved values of shear bond strength.

scholarly journals Effect and mechanisms of reproductive tract infection on oxidative stress parameters, sperm DNA fragmentation, and semen quality in infertile males

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Kang-Sheng Liu ◽  
Xiao-Dong Mao ◽  
Feng Pan ◽  
Rui Fang An
Keyword(s):  
Reproductive Medicine ◽  
Reproductive Tract ◽  
Group Iv ◽  
Semen Parameters ◽  
Group Iii ◽  
Reproductive Tract Infection ◽  
Group I ◽  
Sperm Dna ◽  
Group Ii ◽  
Elastase Level

AbstractRecent years have seen a rising incidence of male infertility, mostly caused by the decline of sperm quality. The ratio of infertile males to infertile females has escalated from 3:7 in 2013 to current 5:5, which turns male infertility into the research focus of reproductive medicine. This study aimed to clarify the effect of reproductive tract infection by ureaplasma urealyticum (UU) and chlamydia trachomatis (CT) on the DNA integrity and routine semen parameters of infertile males. A retrospective study was performed. A total of 259 infertile males who were treated at the Andrological Laboratory Examination and Reproductive Medicine Center in our hospital were analyzed. qRT-PCR was used to examine the infection status of CT and UU. According to the eligibility criteria, we evaluated the semen parameters and biochemical data of 253 men. Based on the results of PCR, the subjects were divided into four groups: Group I (CT positive, 63 cases), Group II (UU positive, 60 cases), Group III (CT positive and UU positive, 62 cases), and Group IV (no infection, 68 cases). DNA fragmentation index (DFI), sperm count, vitality and morphology, elastase level, seminal plasma malondialdehyde (MDA), and total antioxidant capacity (TAC) were assessed. Compared to Group IV, three groups (Group I, Group II and Group III) showed difference in semen volume, proportion of sperm with normal morphology, sperm motility, progressive motility, and vitality (P < 0.05). Compared to Group IV, Group II and Group III showed difference in DFI (P < 0.05). Compared to Group IV, Group II and Group III showed difference in elastase level (P < 0.05). VCL, VSL, VAP, WOB, ROS, TM, HDS showed differences between groups of abnormal/normal WBC (*P < 0.01).UU infection significantly increased the level of seminal leukocytes only in Group II, but not in the other three groups, indicating that UU is a factor to increase the level of seminal leukocytes. Compared with the normal leukocyte group, there were significant differences in total motility, forward motility and normal sperm ratio between the two groups. The proportion of sperm with abnormal morphology (mostly in the head) showed obvious difference between groups of high and normal seminal leukocytic levels. At the same time, in this study, SCGE and SCD verified that leukocytes could damage sperm DNA by increasing ROS, which ultimately affects male fertility.

A Study on the Effect of Samoa Extract (Cleome droserifolia) on Sperm Quality in Male Albino Rats Exposed to Pyrethroid

2021 ◽  
pp. 39-45
Author(s):  
Nura I. Al-Zail ◽  
Salah F. Kamies
Keyword(s):  
Sperm Quality ◽  
Group Iv ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Protective Agent ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Induced Changes

Pyrethroid cyhalothrin (PC) is an insecticide that is used worldwide for pest control in agriculture and household use. Samoa extract (SE) is a potent antioxidant protecting cells from oxidative stress. The present study investigates the protective and therapeutic effect of SE on PC-induced changes in sperm quality in male rats. Fifty adult male albino rats were divided into five groups: group I: served as control; group II: received PC i.p. only (6.2 mg/kg b.wt.); group III: received SE only (100 mg/kg b.wt., p.o.) for eight weeks; group IV: received SE as a protective agent daily for eight weeks, then followed by the administration of PC (i.p.) three times a week for two weeks; group V: exposed to PC (i.p.) three times a week for two weeks, then treated with the SE daily for 8 weeks. Results showed that PC caused markedly impaired sperm quality (a count, viability, motility, and abnormality). Compared to PC-treated animals, SE in the protective group markedly restored the alteration of sperm indices. However, SE in the curative group was found to be less effective in restoring PC-induced alterations. In conclusion, the data of this study revealed that the SE as a protective agent is more effective than as a therapeutic agent. Keywords: Samoa; Pyrethroid; Sperm quality; Rat

Effect of amino acid infusion on renal hemodynamics in humans: a dose-response study

1994 ◽  
Vol 267 (5) ◽  
pp. F703-F708 ◽  
Author(s):  
M. Giordano ◽  
P. Castellino ◽  
E. L. McConnell ◽  
R. A. DeFronzo
Keyword(s):  
Amino Acid ◽  
Dose Response ◽  
Renal Hemodynamics ◽  
Group Iv ◽  
Group V ◽  
Group Iii ◽  
Amino Acid Infusion ◽  
Group I ◽  
Basal Plasma ◽  
Group Ii

We evaluated the dose-response relationship between the plasma amino acid (AA) concentration and renal hemodynamics in eight normal subjects. After an overnight fast, a balanced 10% AA solution was infused for 180 min at five separate infusion rates: 0.5 (group I), 1.0 (group II), 2.0 (group III), 4.0 (group IV), and 6.0 (group V) ml.kg-1.min-1 on separate days. Basal plasma AA concentration was 1.87 +/- 0.1 mmol/l and increased to 2.26 +/- 0.1 (group I), 2.66 +/- 0.2 (group II), 3.79 +/- 0.5 (group III), 5.81 +/- 0.4 (group IV), and 7.41 +/- 0.4 mmol/l (group V). Basal glomerular filtration rate (GFR) and renal plasma flow (RPF) averaged 95 +/- 4 and 476 +/- 29 ml.1.73 m-2.min-1, respectively, and rose to 98 +/- 5 and 506 +/- 40 (group I) [P = not significant (NS)], 102 +/- 3 and 533 +/- 30 (group II) (P < 0.05 vs. basal), 110 +/- 4 and 567 +/- 29 (group III), 115 +/- 7 and 610 +/- 55 (group IV), and 117 +/- 7 and 614 +/- 66 ml.1.73 m-2.min-1 (group V) (P = NS vs. group IV). Basal plasma glucagon concentration averaged 68 +/- 10 pg/ml and increased to 74 +/- 10 (group I), 83 +/- 11 (group II) (P < 0.05 vs. basal), 100 +/- 14 (group III), 121 +/- 14 (group IV), and 229 +/- 35 pg/ml (group V) (P < 0.01 vs. basal). Increases in plasma growth hormone (GH) and insulin levels were observed only during groups IV and V.(ABSTRACT TRUNCATED AT 250 WORDS)

Validation of tonometric measurement of gut intramural pH during endotoxemia and mesenteric occlusion in pigs

1990 ◽  
Vol 259 (4) ◽  
pp. G519-G523 ◽  
Author(s):  
J. B. Antonsson ◽  
C. C. Boyle ◽  
K. L. Kruithoff ◽  
H. L. Wang ◽  
E. Sacristan ◽  
...  
Keyword(s):  
Correlation Coefficients ◽  
Group Iv ◽  
Low Flow ◽  
Total Occlusion ◽  
Group Iii ◽  
Group I ◽  
Invasive Method ◽  
Tissue Pco2 ◽  
Tissue Acidosis ◽  
The Individual

Tonometry is a minimally invasive method for estimating gastrointestinal intramural pH (pHi). Tissue pH is calculated by using the Henderson-Hasselbalch equation and measurements of arterial [HCO-3] and CO2 tension (PCO3) of saline contained in a Silastic balloon within the lumen of the gut. The validity of the method rests on two key assumptions: 1) PCO2 in saline in the tonometer balloon is similar to tissue PCO2 and 2) tissue and arterial [HCO-3] are similar. To validate this method, ileal pHi measured directly with a microelectrode was compared with pHi estimated tonometrically in four groups of anesthetized pigs. Group I (n = 4) were controls. In group II (n = 4), intestinal tissue acidosis was induced by total occlusion of the superior mesenteric artery (SMA). In group III (n = 5), acidosis was induced by partial occlusion of the SMA. In group IV (n = 4), tissue acidosis was induced by endotoxemia. Agreement was excellent between direct and tonometric measurements in groups I and IV and less good in groups II and III. Weighted mean correlation coefficients (rw) for the two measurement methods were 0.743 and 0.9447 in groups II and IV, respectively. Correlation coefficients for the individual animals in group III were more variable than the other groups and ranged from 0.547 to 0.990. The tonometric method for measuring GI pHi is invalid under conditions of zero flow and leads to error under conditions of low flow. However, the method is reliable in the setting of tissue acidosis induced by endotoxemia.

scholarly journals Effect of concurrent exposure of toxic concentrations of lead and endosulfan on oxidative stress indices in rats

2021 ◽  
Vol 10 (3) ◽  
pp. 192-195
Author(s):  
Ranganathan V ◽  
◽  
Malik JK ◽  
Rao GS ◽  
◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reduced Glutathione ◽  
Group Iv ◽  
Male Rats ◽  
Technical Grade ◽  
Stress Indices ◽  
Group Iii ◽  
Group I ◽  
Male Wistar Rats ◽  
Toxic Concentrations

The effect of concurrent exposure of toxic concentrations of lead and endosulfan were evaluated on oxidative stress parameters in male wistar rats. Group I served as untreated control whereas Group II received drinking water containing lead as lead acetate @1000 ppm (Pb1000). Group III was exposed to feed containing technical grade endosulfan @ 100 ppm (E100). Group IV was exposed to Pb (1000) +E (100). All the treatments were given daily for 28 days. Combination of lead and endosulfan modified the indices of oxidative stress in the parameters such as lipid peroxidation, reduced glutathione, superoxide dismutase and catalase in rats as compared to their individual compounds. The results suggest that the combination of these individual compounds may have the potential to modify oxidative stress produced by single compounds in male rats

scholarly journals FLUORIDE ABSORBED ON EXTRACTED TEETH AFTER IMMERSING IN FLUORIDE TABLET, FLUOCOL SOLUTION AND FLUORIDE DENTRIFICE (IN VITRO TEST)

2016 ◽  
Vol 3 (1) ◽  
pp. 42
Author(s):  
Diyah Fatmasari ◽  
Lanny Sunarjo
Keyword(s):  
Research Result ◽  
Fluoride Concentration ◽  
Group Iv ◽  
In Vitro Test ◽  
Significance Level ◽  
Group Iii ◽  
Group I ◽  
Before And After ◽  
Vitro Test

Background: The role of fluoride in preventing tooth decay both for children and adult has been acknowledged internationally. There are several types of fluoride modalities either topically or systemic way. In Indonesia the modalities used to apply are fluoride tablet, fluocol solution and fluoride containing toothpaste. The purpose of this research is to find the effectiveness of fluoride modalities.Method: The study design was quasy experimental with 40 extracted teeth (Premolar teeth). The teeth divided into four groups D group I soaked on tablet fluor, Group II ; soaked on fluocol solution, Group III soaked on fluoride tooth paste and group IV soaked on Mill J (Aquadest/ Control solution). Fluoride concentration before and after soaking was determined using Spectrophotometer UV-Vis. Fluoride absorption was determined by the reduction of fluoride concentration after soaking.Result: Research result shown that the highest fluoride absorption was on soaking in fluoride tablet, followed by soaking on fluocol and tooth paste (mean fluoride absorption was 0,32; 0,08 and 0,04 ppm). Anova test shown significance level was 0,000.Conclusion: there were a significance of fluoride absorption on soaking in tablet fluoride, fluocol solution and toothpaste. The mechanism of fluoride regimens shown different fluoride absorption.

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