Maternal Supply of Ruminally-Protected Lysine and Methionine During Close-Up Period Enhances Immunity and Growth Rate of Neonatal Calves

The objective of this study was to evaluate the effect of supplying ruminally-protected lysine (RPL), methionine (RPM), or the two in combination (RPML) to transition dairy cows on the immunity and performance of their offspring. Eighty heifer calves (n = 20 calves per group) were assigned to four treatments based on their dam diet; basal diet (CON), a basal diet with lysine [RPL, 0.33% of dry matter (DM)], a basal diet with methionine (RPM, 0.16% DM), or with the combination (RPML). Calves were fed colostrum from their dams within 2 h of birth. Calves were then fed milk only (d 2–22), a combination of milk and milk replacer (d 23–25), and milk replacer (d 25–60). Starter feed was fed to the calves twice daily after liquid feeding. Calves blood samples were collected after calving on 0, 12, 24, and 48 h and 5 and 7 d after birth. Data were analyzed by SAS software v9.4. Providing ruminally-protected amino acids (RPAA) to transition cows improved colostrum quality compared to the CON (Brix; P < 0.01). Serum total protein concentrations were higher in calves from supplemented cows than in calves from unsupplemented cows (P < 0.01). Calves born to dams in the RPM, RPL, and RPML groups had higher plasma immunoglobulin G (IgG) concentrations 0, 12, 24, and 48 h and 7 d after birth than those born to dams in the CON group (P < 0.05). The percentage of calves with adequate passive immunity transfer was increased with RPM and RPL or the two in combination (P < 0.01). However, there was no difference in the percentage of calves with adequate passive immunity transfer between the RPM and RPL groups (P = 0.21). Calves from cows that receive supplemental RPAA have a greater average daily gain (ADG) than those born to cows in the CON group (P < 0.01). These results indicate that maternal supplementation with RPM or RPL or the two in combination during the periparturient period could be an alternative strategy to improve the performance of calves, especially in accelerated growth programs in calves.

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Effects of nucleotide supplementation to corn–soybean meal-based diet on growth performance, fecal microflora, and blood profiles of sows and performance of suckling piglets

Canadian Journal of Animal Science ◽

10.1139/cjas-2018-0222 ◽

2019 ◽

Vol 99 (4) ◽

pp. 754-763 ◽

Cited By ~ 1

Author(s):

Y. Jiao ◽

S.D. Upadhaya ◽

I.H. Kim

Keyword(s):

Growth Performance ◽

Average Daily Gain ◽

Basal Diet ◽

Linear Increase ◽

Fecal Microflora ◽

Suckling Piglets ◽

And Performance ◽

Dietary Treatments ◽

Piglet Survival ◽

Blood Profiles

A total of 45 sows (Landrace × Yorkshire) and their litters were used in this study to evaluate the efficacy of nucleotide supplementation on reproductive performance, growth performance, fecal microflora, and blood profiles in sows and piglets. Sows were allocated to one of three treatments (15 pigs per treatment and the average parity was 4.13). Dietary treatments consisted of (1) CON: basal diet, (2) T1: CON + 0.5% nucleotides, and (3) T2: CON + 1.0% nucleotides. In this study, the average daily feed intake during lactation and back fat at weaning day was increased linearly (P < 0.05) in sows with the increase in nucleotide supplementation levels. Sows receiving increasing levels of nucleotides had linearly increased fecal Lactobacillus counts and decreased Escherichia coli counts at weaning day (P < 0.05). Linear reduction of the epinephrine, norepinephrine, and cortisol in sows was observed at weaning day, whereas superoxide dismutase was increased linearly (P < 0.05). Body weight and average daily gain in piglets were linearly (P < 0.05) increased with the increasing levels of nucleotides. Linear increase (P < 0.05) was also observed on total piglets born, live piglets, and piglet survival. The current study demonstrated the importance of nucleotides in the diets of sows to improve sow and piglet performance.

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Performance and behaviour of calves reared in groups or individually following an enhanced-growth feeding programme

Journal of Dairy Research ◽

10.1017/s0022029906002019 ◽

2006 ◽

Vol 73 (4) ◽

pp. 480-486 ◽

Cited By ~ 13

Author(s):

Marta Terré ◽

Alex Bach ◽

Maria Devant

Keyword(s):

Dry Matter ◽

Average Daily Gain ◽

Dry Matter Intake ◽

Milk Replacer ◽

Serum Urea ◽

Nefa Concentration ◽

Unlimited Access ◽

And Performance ◽

Oral Behaviour ◽

Daily Gain

Nineteen calves were reared in individual pens (IP), and twenty calves were grouped in four pens of five calves each (GP) to study the effects of grouping on behaviour and performance. All calves were on an enhanced-growth feeding programme and had unlimited access to starter. Total dry matter intake (DMI) body weight (BW), serum urea, non-esterified fatty acid (NEFA), cortisol, insulin, and glucose concentrations, and immune response to vaccination were measured. Behaviour patterns categorized as: non-nutritive oral behaviour, cross-sucking, inter-sucking, and self-grooming, were monitored 20 min following the morning and afternoon milk replacer consumption, and at the same times after weaning. There were no differences in final BW and starter DMI between treatments. The decrease of average daily gain (ADG) during the week following weaning was more pronounced in GP than in IP calves. Serum NEFA concentration increased at the beginning of the study in IP calves but serum urea concentrations were greater in GP than in IP calves the week after weaning. Self-grooming increased in both treatments throughout the study but GP calves increased non-nutritive oral behaviour and decreased cross-sucking and inter-sucking behaviours throughout the study. Rearing calves in groups following an enhanced-growth feeding programme did not increase starter intake. Furthermore, cross-sucking and inter-sucking behaviours in calves reared in groups did not seem to cause increased metabolic or immunological distress.

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Effects of species-specific probiotic addition to milk replacer on calf health and performance during the first month of life

Annals of Animal Science ◽

10.2478/aoas-2013-0089 ◽

2014 ◽

Vol 14 (1) ◽

pp. 101-115 ◽

Cited By ~ 12

Author(s):

Alessandro Agazzi ◽

Erica Tirloni ◽

Simone Stella ◽

Serena Maroccolo ◽

Barbara Ripamonti ◽

...

Keyword(s):

Immune Response ◽

Body Weight ◽

Growth Performance ◽

Average Daily Gain ◽

Bacillus Coagulans ◽

Basal Diet ◽

Milk Replacer ◽

Blood Eosinophil ◽

Feed Conversion ◽

Species Specific

AbstractThe aim of this study was to evaluate the effects of the administration of a species-specific probiotic (Lactobacillus animalis SB310, Lactobacillus paracasei subsp. paracasei SB137 and Bacillus coagulans SB117 in a 30:35:35 ratio, respectively; 1.8 × 1010 CFU/g of powder) on gut microbial balance, immune response and growth performance of Holstein female calves during the first month of life. Twenty-two calves were divided into two experimental groups from 2 to 28 days of life: control (C), fed with milk replacer and concentrate as a basal diet, and treatment (T), fed C diet plus 1 g/calf/day of probiotic powder for the first month of age. Faecal and blood samples were individually collected and analysed weekly. Individual faecal score was recorded daily and general health score was calculated at the end of the trial. Cell-mediated immune response was evaluated by skin test at 7 and 28 days of life. Milk replacer and concentrate intake were recorded daily, while body weight and biometrical parameters were recorded at 2, 8, 14, 21 and 28 days of life, thus average daily gain and feed conversion rate were calculated. During the first week of treatment, lower blood eosinophil percentage (0.05% vs. 0.22%; P≤0.01) was found in T group, while basophils were higher in T than C group at the end of the trial (0.21% vs. 0.16%; P≤0.05). Higher faecal lactic acid bacteria (LAB)/E. coli ratio on day 28 of life (3.73 log CFU/g vs. 2.02 log CFU/g; P≤0.05) and lower incidence of diarrhoea were found in the treated group (63.30% vs. 70.71%; P=0.05). Body weight (48.92 kg vs. 46.92 kg; P≤0.05), total concentrate intake (14.77 kg vs. 12.56 kg on dry matter basis; P≤0.05), and heart girth (81.16 cm vs. 78.49 cm; P≤0.05) were significantly higher in T group. The administration of the probiotic during the first month of life improved gut microbiota and increased the growth performance and some biometric parameters of calves.

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Administration of loperamide and addition of wheat bran to the diets of weaner pigs decrease the incidence of diarrhoea and enhance their gut maturation

British Journal Of Nutrition ◽

10.1017/s0007114509992637 ◽

2009 ◽

Vol 103 (6) ◽

pp. 879-885 ◽

Cited By ~ 17

Author(s):

Francesc Molist Gasa ◽

Mauro Ywazaki ◽

Arantza Gómez de Segura Ugalde ◽

Rafael Gustavo Hermes ◽

Josep Gasa Gasó ◽

...

Keyword(s):

Transit Time ◽

Wheat Bran ◽

Control Diet ◽

Animal Health ◽

Average Daily Gain ◽

Basal Diet ◽

Protein Digestibility ◽

Intestinal Morphology ◽

Weaned Pigs ◽

And Performance

The influence of fibre inclusion and transit time regulation on the performance, health status, microbial activity and population, physico-chemical characteristics of the hindgut digesta and intestinal morphology in early weaned pigs were examined. For these experiments, wheat bran (WB) was used as fibre source and loperamide as a drug (LOP) to increase the digesta transit time. In Expt 1, a total of 128 early weaned pigs were randomly distributed in a 2 × 2 factorial combination of WB inclusion (0v.40 g/kg) and LOP administration (0v.0·07 mg/kg body weight) during 13 d. For Expt 2, a total of twenty-four piglets were allotted to three dietary treatments for 15 d with the same basal diet (control diet) as Expt 1; a diet with 80 g/kg of WB and the combination of WB and LOP. In Expt 1, LOP improved the average daily feed intake and average daily gain of the animals (P = 0·001 and 0·007, respectively). The same result was obtained when WB was combined with LOP. The WB–LOP group also showed a higher concentration of SCFA (P = 0·013), acetic acid (P = 0·004) and propionic acid (P = 0·093). On the other hand, WB inclusion reduced the organic matter and crude protein digestibility (P = 0·001) and tended to decrease the enterobacteria population (P = 0·089). In Expt 2, WB increased the butyric acid concentration (P = 0·086). We concluded that the inclusion of WB to modify the intestinal microbiota activity combined with LOP may be beneficial to animal health and performance.

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Effects of Maternal Supplementation with Rare Earth Elements during Late Gestation and Lactation on Performances, Health, and Fecal Microbiota of the Sows and Their Offspring

Animals ◽

10.3390/ani9100738 ◽

2019 ◽

Vol 9 (10) ◽

pp. 738 ◽

Cited By ~ 2

Author(s):

Yi Xiong ◽

Jiaman Pang ◽

Liangkang Lv ◽

Yujun Wu ◽

Na Li ◽

...

Keyword(s):

Rare Earth ◽

Rare Earth Elements ◽

Average Daily Gain ◽

Basal Diet ◽

Fecal Microbiota ◽

Late Gestation ◽

Inflammatory Factors ◽

Control Group ◽

Maternal Supplementation ◽

Daily Gain

The study was conducted to investigate the effects of maternal supplementation with rare earth elements (REEs) on sows and their offspring. During late gestation, 120 multiparous sows were divided randomly into the control group (Basal diet) and REE-G group (Basal diet supplemented with 200 mg REE/kg). After delivery, REE-G group was further divided into two groups: REE-L- (Change to basal diet during lactation) and REE-L+ group (REE diet all the time). Our results showed that maternal REE supplementation improved the antioxidant and immunity of sows and piglets. Additionally, REE supply during late gestation significantly decreased the coefficient of within-litter variation (CV) in birth weight and increased the weaning weights and the average daily gain (ADG) of piglets. During lactation, the insulin-like growth factor-1 (IGF-1) levels in piglets of REE-L+ group were higher, while no difference between REE-L- and the control group. More beneficial bacteria (Christensenellaceae and Ruminococcaceae) were found in the REE-L+ group while some opportunistic pathogens (Proteobacteria and Campylobacter) were relatively suppressed. Fecal microbiota showed correlation with antioxidase, inflammatory factors, and average daily gain (ADG). Collectively, our findings indicated that REEs added in both gestation and lactation was more conducive to establish a healthier status for sows and their offspring.

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Impact of 2 Versus 1 Colostrum Meals on Failure of Transfer of Passive Immunity, Pre-Weaning Morbidity and Mortality, and Performance of Dairy Calves in a Large Dairy Herd

Animals ◽

10.3390/ani11030782 ◽

2021 ◽

Vol 11 (3) ◽

pp. 782

Author(s):

Angel Abuelo ◽

Faith Cullens ◽

Amanda Hanes ◽

Jill L. Brester

Keyword(s):

Average Daily Gain ◽

Dairy Herd ◽

Morbidity And Mortality ◽

Dairy Calves ◽

Passive Immunity ◽

Lower Probability ◽

Linear Regression Models ◽

Mortality And Morbidity ◽

And Performance ◽

The Impact

Failure of transfer of passive immunity (FTPI) due to inadequate ingestion of colostral immunoglobulins by calves is associated with increased mortality and morbidity risks. Feeding calves a sufficient amount of quality-tested colostrum within the first hours of life is essential for successfully transferring passive immunity. Many farms have implemented a second meal of colostrum to maximize the opportunities for passive immunity transfer. However, excellent passive immunity can be achieved with a single feeding of sufficient quality-tested colostrum. Moreover, there is currently no evidence demonstrating the impact of a second colostrum feeding within 24 h of life in calves receiving adequate volumes of quality-tested colostrum in an initial feeding. Hence, the objective of this retrospective cohort study was to compare the risks of FPTI, pre-weaning morbidity and mortality, and growth and performance between dairy calves that received one or two feedings of colostrum. For this, the health and production records of a large dairy herd were analyzed. At this farm, newborn calves receive 3 L of quality-tested colostrum soon after birth, followed by another 2 L 5–6 h later. However, at times of shortages of colostrum, calves only receive the initial 3 L meal. The records of 2064 male and 2272 female calves were analyzed, where 4156 and 180 calves received 2 and 1 colostrum meals, respectively. Data from both sexes were included in the analysis of the risks of FTPI, morbidity, and mortality; however, only data from heifer calves were utilized for growth and performance analysis. Survival analysis, and logistic and linear regression models were used to investigate the impact of receiving two feedings of colostrum on calf FTPI status, morbidity, mortality, reproductive indices, pre-weaning average daily gain (ADG), and first lactation 305-d Mature Equivalent milk production (305ME). Calves that received two feedings of colostrum had lower odds of FTPI, a lower probability of being treated for respiratory disease, diarrhea, or any disease, and a greater pre-weaning ADG. However, there was no association between the number of colostrum feedings and pre-weaning mortality, and the probabilities of first insemination and first calving, although heifers receiving two colostrum feedings tended to receive fewer inseminations and to have a greater first lactation 305ME. Collectively, our results suggest that feeding calves a second feeding of colostrum 5–6 h after the initial feeding soon after birth could be an effective strategy to decrease FTPI and morbidity and optimize ADG in dairy calves pre-weaning.

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An investigation into the effects of maternal supplementation with excess iodine on the mechanisms and impacts of reduced IgG absorption in the lamb postpartum

British Journal Of Nutrition ◽

10.1017/s0007114517000575 ◽

2017 ◽

Vol 117 (7) ◽

pp. 951-963 ◽

Cited By ~ 1

Author(s):

F. M. McGovern ◽

T. Sweeney ◽

M. T. Ryan ◽

S. Lott ◽

F. P. Campion ◽

...

Keyword(s):

Thyroid Hormone ◽

Thyroid Hormone Receptor ◽

Average Daily Gain ◽

Basal Diet ◽

Excess Iodine ◽

Serum Igg ◽

Faecal Egg Count ◽

Hormone Status ◽

Maternal Supplementation ◽

Daily Gain

AbstractAn experiment was conducted to determine: (1) the effect of excess maternal I supplementation on the thyroid hormone status of the ewe and her progeny; (2) potential mechanisms underpinning the failure of passive transfer associated with excess I and (3) the growing lambs’ response to natural gastrointestinal infection. Twin-bearing ewes received one of two treatments (n 32/treatment group): basal diet (C) or C plus 26·6 mg of iodine/ewe per d (I), supplied as calcium iodate. Ewes were individually fed from day 119 of gestation to parturition. Progeny of I ewes had lower (P<0·01) serum IgG concentrations from 24 h to 28 d postpartum but higher serum IgG concentrations at day 70 postpartum (P<0·05). I supplementation increased the relative expression of Fc receptor, IgA, IgM high affinity and polymeric Ig receptor in the ileum of the lamb at 24 h postpartum; however, thyroid hormone receptor-β (THRB) and β-2-microglobulin (B2M) expression declined (P<0·05). Progeny of I ewes had higher growth rates to weaning (P<0·05) and lower faecal egg count (FEC) for Nematodirus battus (P<0·05) between weeks 6 and 10 postpartum. In conclusion, excess maternal I supplementation negatively affected the thyroid hormone status, serum IgG concentration, ileal morphology and the gene expression of THRB and B2M in the ileum and ras-related protein (RAB) RAB25 and the mucin gene (MUC) MUC1 in the duodenum of the lamb postpartum. These effects were followed by an enhancement of average daily gain and lower N. battus FEC in the pre-weaning period of I-supplemented lambs.

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Isoquinoline Alkaloids in Sows’ Diet Reduces Body Weight Loss during Lactation and Increases IgG in Colostrum

Animals ◽

10.3390/ani11082195 ◽

2021 ◽

Vol 11 (8) ◽

pp. 2195

Author(s):

Ester Arévalo Sureda ◽

Xuemei Zhao ◽

Valeria Artuso-Ponte ◽

Sophie-Charlotte Wall ◽

Bing Li ◽

...

Keyword(s):

Gene Expression ◽

Weight Loss ◽

Body Weight ◽

Body Weight Loss ◽

Muscle Thickness ◽

Passive Immunity ◽

Isoquinoline Alkaloids ◽

Back Muscle ◽

Fat Thickness ◽

And Performance

Isoquinoline alkaloids (IQ) exert beneficial antimicrobial and anti-inflammatory effects in livestock. Therefore, we hypothesized that supplementing sows’ diets with IQ during gestation would decrease farrowing stress, affecting the piglets’ development and performance. Sows were divided into: IQ1, supplemented with IQ from gestation day 80 (G80) to weaning; IQ2, supplemented from gestation day 110 (G110) to weaning, and a non-supplemented (NC) group. Sow body weight (BW), feed intake, back-fat thickness and back-muscle thickness were monitored. Cortisol, glucose and insulin were measured in sows’ blood collected 5 d before, during, and after 7 d farrowing. Protein, fat, IgA and IgG were analyzed in the colostrum and milk. Piglets were monitored for weight and diarrhea score, and for ileum histology and gene expression 5 d post-weaning. IQ-supplemented sows lost less BW during lactation. Glucose and insulin levels were lower in the IQ groups compared to NC-sows 5 d before farrowing and had higher levels of protein and IgG in their colostrum. No other differences were observed in sows, nor in the measured parameters in piglets. In conclusion, IQ supplementation affected sows’ metabolism, reducing body weight loss during lactation. Providing IQ to sows from their entrance into the maternity barn might be sufficient to induce these effects. IQ improved colostrum quality, increasing the protein and IgG content, improving passive immunity for piglets.

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109 Phytate supplementation has minimal impact on mineral digestibility in horses

Journal of Animal Science ◽

10.1093/jas/skaa278.163 ◽

2020 ◽

Vol 98 (Supplement_4) ◽

pp. 89-90

Author(s):

Katy Brinkley-Bissinger ◽

Laura M Cersosimo ◽

Kathleen E Sullivan ◽

Shannon E Livingston ◽

Jill M Bobel ◽

...

Keyword(s):

Inductively Coupled Plasma ◽

Mixed Model ◽

Divalent Cations ◽

Basal Diet ◽

Minimal Impact ◽

Inductively Coupled ◽

Mixed Model Anova ◽

Microbial Phytase ◽

And Performance ◽

Inorganic Mineral

Abstract Phosphorus in equine rations is supplied by inorganic mineral fortification or naturally-occurring P in forages and grains. Up to 70% of P in plant material is bound to phytate. In monogastrics like poultry and swine, phytate can reduce absorption of P and divalent cations, but the extent that this occurs in horses is unknown. This study tested the hypothesis that phytate decreases mineral digestibility in horses. Six mature Quarter Horse geldings (mean ± SE, 586 ± 19 kg, 10 ± 1.5 y) were randomly assigned to two treatments applied in a cross-over design: IP6 (Ca-Mg-phytate isolated from rice bran fed at 15 mg phytic acid/kg BW) or CON (equivalent Ca, Mg and P from inorganic minerals to match intake from IP6). The level of phytate added represented an amount present in grain-rich rations typically fed to broodmares, growing horses and performance athletes. Supplements were added to a basal diet (1.75% BW, DM basis) consisting of 75% timothy hay and 25% roughage-based concentrate. Each 14-d period had an 11-d treatment adaptation followed by a 3-d total fecal collection. After acid digestion, P was determined colorimetrically and other minerals were determined by inductively coupled plasma spectrometry. Data were analyzed using mixed model ANOVA. Intakes of Ca, P, Mg, Zn, Cu and Fe were similar between treatments (140, 72, 40, 1.05, 0.24, and 1.78 mg/kg BW respectively). Apparent P digestibility (18.8 and 17%, SEM 1.9; P = 0.41) and estimates of true P digestibility (32.8 and 30.8%, SEM 1.9; P = 0.39) were similar between CON and IP6. Apparent digestibilities of other minerals were also not affected by IP6 supplementation. Findings suggest horses have sufficient microbial phytase activity in the gastrointestinal tract to mitigate impacts of dietary phytate. Higher levels or different forms of phytate and marginal mineral intake may yield different results.

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Effects of Dietary Supplementation of gEGF on the Growth Performance and Immunity of Broilers

Animals ◽

10.3390/ani11051394 ◽

2021 ◽

Vol 11 (5) ◽

pp. 1394

Author(s):

Jianyong Zhou ◽

Jingyi Yao ◽

Luhong Bai ◽

Chuansong Sun ◽

Jianjun Lu

Keyword(s):

Antioxidant Capacity ◽

Growth Performance ◽

Crude Extract ◽

Serum Alkaline Phosphatase ◽

Average Daily Gain ◽

Basal Diet ◽

Control Group ◽

Serum Samples ◽

Chicken Embryos ◽

Growth Of Animals

EGF has been shown to stimulate the growth of animals. In this study, the content of EGF in chicken embryos (gallus EGF, gEGF) aged from 1 to 20 days of incubation were determined by ELISA kit, and the 5-day-old chicken embryos with the highest content of 5593 pg/g were selected to make gEGF crude extracts. A total of 1500 1-day-old Xianju chickens were randomly divided into five groups with six replicates of 50 chickens each. The control group was fed a basal diet, and other treatment diets were supplemented with 4, 8, 16 and 32 ng/kg gEGF crude extract, respectively. The experiment lasted for 30 days. Chicks were harvested at the end of the experiment, and liver, spleen, thymus, bursa and serum samples were collected. Results showed that average daily gain (ADG) and average daily feed intake (ADFI) of 16 ng/kg group were higher than those in the control group (p < 0.05). The serum uric acid (UA) of the 16 ng/kg group was reduced (p < 0.01), and the serum alkaline phosphatase (AKP) of the 16 ng/kg group increased (p < 0.01). The gEGF extract also increased chick’s antioxidant capacity, decreased malondialdehyde (MDA) and increased catalase (CAT) in the liver and serum of 16 ng/kg groups in compared to the control group (p < 0.01). Furthermore, immunity was improved by the addition of gEGF to broiler diets. The serum immunoglobin A (IgA) content of 8 and 16 ng/kg groups and the serum immunoglobin M (IgM) content of 4 and 8 ng/kg groups were increased (p < 0.05) compared to the control group. The bursa index of each experimental group was higher than the control group (p < 0.01). These findings demonstrate that the crude extract of gEGF prepared in this experiment could improve the growth performance, antioxidant capacity and immunity of broilers.

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