scholarly journals Response Surface Modeling and Optimization of Polyphenols Extraction from Apple Pomace Based on Nonionic Emulsifiers

Agronomy ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 92
Author(s):  
Liubov Skrypnik ◽  
Anastasia Novikova
Keyword(s):  
Aqueous Solution ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Polynomial Regression ◽  
Tween 80 ◽  
Pharmaceutical Products ◽  
Apple Pomace ◽  
Tween 20 ◽  
Total Phenolic ◽  
Extraction Technology

The factors affecting the efficiency of micelle-mediated extraction of phenolic compounds from apple pomace was investigated. Higher extraction efficiency by using as a solvent an aqueous solution of Tween 80 in comparison to Triton X-100, Span 20, Tween 20, 70% ethanol, and water was shown. Four independent variables (Tween 80 concentration, time, solvent-to material ratio, and pH) to enhance the recovery of polyphenols from apple pomace was investigated. Applying response surface methodology, the second order polynomial regression equation showing dependence of the yield of polyphenols on the extraction parameters was derived. The adjusted regression coefficient (R2 = 98.73%) and the lack-of-fit test (p > 0.05) showed a good accuracy of the developed model. The difference between observed and predicted values was no more than 3%. The optimal extraction conditions were found to be Tween 80 concentration of 1.14%, time of 65 min, solvent-to-material ratio of 104 mL g−1, pH of 3.8. Under optimal conditions the predicted total phenolic compounds content was 7.75 mg g−1. The obtained apple pomace extracts were characterized in terms of their antioxidant activity. The proposed extraction technology by using Tween 80 aqueous solution as a solvent allows obtaining the extracts with high content of polyphenols which are suitable for the applications in food, cosmetic, and pharmaceutical products requiring a surfactant to stabilize them.

scholarly journals Phenolic Compounds Extraction of Arbutus unedo L.: Process Intensification by Microwave Pretreatment

Processes ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 298 ◽  
Author(s):  
Agostinho M. R. C. Alexandre ◽  
Ana A. Matias ◽  
Maria Rosário Bronze ◽  
Maria Jose Cocero ◽  
Rafael Mato
Keyword(s):  
Phenolic Compounds ◽  
Extraction Procedure ◽  
Process Intensification ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Extraction Technology ◽  
Kinetic Evaluation ◽  
Total Anthocyanin ◽  
Arbutus Unedo ◽  
Short Time

Arbutus unedo L., commonly known as the strawberry-tree fruit, is an endemic species of the Mediterranean flora. Microwave extraction technology has been considered as a fast and “green” method for the production of extracts rich in bioactive compounds, although the energy consumption is high. To overcome this bottleneck, microwave was used as a pretreatment procedure in short time periods. This technique promotes the burst of intracellular vacuoles leading to an increase in the lixiviation of phenolic compounds. Different approaches were tested, namely a solvent-free irradiation (SFI), a solvent-assisted irradiation (SAI) and a pressurized solvent-assisted irradiation (PSAI). After irradiation, a solid–liquid extraction procedure was performed using a mixture of water and ethanol. A kinetic evaluation of the total phenolic content (TPC) was performed using the Folin–Ciocalteu method. For the total anthocyanin content, a UV-spectrophotometric method was used. HPLC-UV and LC-MS were used for TPC and identification of present compounds. Microwave irradiation led to an increase in TPC of extracts after SAI (52%) and PSAI (66%) along with a reduction in time of extraction from 30 min to less than 2 min. The anthocyanin content also increased by 66% for the SAI and PSAI extractions.

Determination of optimal extraction conditions for phenolic compounds from Pistacia atlantica leaves using the response surface methodology

2016 ◽  
Vol 8 (31) ◽  
pp. 6107-6114 ◽  
Author(s):  
Ziyad Ben Ahmed ◽  
Mohamed Yousfi ◽  
Johan Viaene ◽  
Bieke Dejaegher ◽  
Kristiaan Demeyer ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Box Behnken Design ◽  
Pistacia Atlantica

Response surface methodology in combination with a Box–Behnken design was performed to optimize the extraction conditions, resulting in a maximum total phenolic content yield from Pistacia atlantica leaves.

scholarly journals Optimization of phenolic compounds extraction with antioxidant activity from açaí, blueberry and goji berry using response surface methodology

2018 ◽  
pp. 180 ◽  
Author(s):  
Cristiane de Moura, Amália Soares dos Reis ◽  
Letícia Dangui da Silva, Vanderlei Aparecido de Lima ◽  
Tatiane Luiza Cadorin Oldoni ◽  
Celeide Pereira ◽  
Solange Teresinha Carpes
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
High Performance ◽  
Total Phenolic ◽  
Global Response ◽  
Goji Berry ◽  
Berry Fruits

The aim of this study was to determine the best extraction conditions of phenolic compounds present in açaí, blueberry and goji berry fruits using the response surface methodology (RSM). The phenolic compounds profile by high-performance liquid chromatography, antioxidant activity was also determined. A factorial 23 design was used to analyze the effect of the solvent (ethanol and water), time (30 and 60 min) and temperature (30 °C and 60 °C) on the extraction of total phenolic compounds (TPC) and activity antioxidant (AA). The variables time and temperature had a positive effect on antioxidant activity (AA) in their highest levels, 60 min and 60 °C, respectively. The solvent ethanol 800 g/mL was more efficient in TPC extracting with AA in all matrices. Rutin was present in high amounts in blueberry and goji berry, and the myricetin in açaí. The açaí showed higher in vitro antioxidant activity when extracted at 60 °C for 60 min. The high correlation coefficient (0.98) of global response (GR) showed that we can find out single and global response in research with multiple dependent variables.  The GR analysis indicated the highest values of the TPC and AA when the fruits were extracted at 60°C for 60 min using ethanol as solvent and it was very useful for simplifying and improving the phenolic compounds extraction performance.

Optimization of Supercritical Fluid Extraction of Phenolic Compounds from Peach Blossom (Amygdalus Persica) by Response Surface Methodology

2018 ◽  
Vol 17 (2) ◽  
pp. 180-187
Author(s):  
Yang Wen-Bo ◽  
Liu Jie-Chao ◽  
Liu Hui ◽  
Zhang Chun-Ling ◽  
Lv Zhen-Zhen ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Fluid Extraction ◽  
Phenolic Contents ◽  
C 30

Supercritical fluid extraction (SFE) was used for the extraction of phenolic compounds from peach blossom (Amygdalus persica). The optimum conditions of SFE were optimized by response surface methodology and confirmed at 64°C, 30 MPa, 143 min, and 35 mL ethanol (100%) as modifier for maximum total phenolic contents (54.10 mg GAE g−1 DW), which was higher than the yield achieved by ultrasonic-assisted extraction (44.04 mg GAE g−1 DW). The antioxidant activities and tyrosinase inhibitory activities of extraction extracted by SFE were 79.55% and 14.42%. Furthermore, chlorogenic acid, quercetin-3-O-galactoside, kaempferol-3-O-galactoside, quercitrin-3-O-rhamnoside, and kaempferol 4′-glucoside were identified in this study.

scholarly journals Comparative Evaluation of Four Extraction Methods of Antioxidant Compounds from Decatropis bicolor in Aqueous Medium Applying Response Surface Design

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1042
Author(s):  
Judith Jaimez-Ordaz ◽  
Elizabeth Contreras-López ◽  
Tania Hernández-Sánchez ◽  
Luis Guillermo González-Olivares ◽  
Javier Añorve-Morga ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Aqueous Medium ◽  
Response Surface ◽  
Extraction Methods ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Physical Factors ◽  
Antioxidant Power ◽  
Response Surface Designs ◽  
Ferric Reducing Antioxidant Power

The objective of this paper is to compare conventional, ultrasound, microwave, and French press methods for the extraction of antioxidant compounds from Decatropis bicolor in an aqueous medium. This plant is widely used in Mexican traditional medicine for breast cancer treatment. Despite that, there are few studies on D. bicolor. Two response surface designs were applied to establish the best conditions of the liberation of antioxidants from D. bicolor, which were determined by DPPH• and Ferric Reducing Antioxidant Power (FRAP) techniques. The total phenolic content was evaluated by the Folin-Ciocalteu method. The results showed that D. bicolor is a source of antioxidants (669–2128 mg ET/100 g and 553–1920 mg EFe2+/100 g, respectively) and phenolic compounds (2232–9929 mg EGA/100 g). Among the physical factors that were analyzed, the temperature was the determinant factor to liberate the compounds of interest by using low concentrations of the sample and short times of extraction. The French press was the most efficient method, obtaining values of antioxidant activity and phenolic compounds even higher than those reported by using extraction methods with solvents such as methanol.

scholarly journals Extraction of Phenolic Compounds from Fresh Apple Pomace by Different Non-Conventional Techniques

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4272
Author(s):  
Luna Pollini ◽  
Lina Cossignani ◽  
Cristina Juan ◽  
Jordi Mañes
Keyword(s):  
Phenolic Compounds ◽  
Value Added ◽  
Apple Pomace ◽  
Total Phenolic ◽  
Ultrasound Assisted Extraction ◽  
A Value ◽  
Assisted Extraction ◽  
Starting Point ◽  
Apple Products ◽  
Pre Treatment

Red Delicious apple pomace was produced at laboratory scale with a domestic blender and different non-conventional extraction techniques were performed to isolate phenolic compounds, such as ultrasound-assisted extraction (UAE), ultraturrax extraction (UTE), accelerated solvent extraction (ASE) and pulsed electric field (PEF) extraction pre-treatment. Total phenolic content (TPC) was determined by Folin–Ciocalteu assay. Phloridzin, the main phenolic compound in apples, was determined by chromatographic analysis Q-TOF-LC/MS. The results obtained with these techniques were compared in order to identify the most efficient method to recover polyphenols. The highest value of TPC (1062.92 ± 59.80 µg GAE/g fresh apple pomace) was obtained when UAE was performed with EtOH:H2O (50:50, v/v), while ASE with EtOH:H2O (30:70, v/v) at 40 °C and 50% of flush was the most efficient technique in the recovery of phloridzin. The concentration of the main phenolic compounds ranged from 385.84 to 650.56 µg/g fresh apple pomace. The obtained results confirm that apple pomace represents an interesti-ng by-product, due to the presence of phenolic compounds. In particular, phloridzin could be considered a biomarker to determine the quality of numerous apple products. Therefore, this research could be a good starting point to develop a value-added product such as a functional food or nutraceutical.

scholarly journals Modelling and Optimization of Ultrasound-Assisted Extraction of Phenolic Compounds from Black Quinoa by Response Surface Methodology

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3616
Author(s):  
Valentina Melini ◽  
Francesca Melini
Keyword(s):  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Optimal Conditions ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Functional Components

Phenolic compounds are currently the most investigated class of functional components in quinoa. However, great variability in their content emerged, because of differences in sample intrinsic and extrinsic characteristics; processing-induced factors; as well as extraction procedures applied. This study aimed to optimize phenolic compound extraction conditions in black quinoa seeds by Response Surface Methodology. An ultrasound-assisted extraction was performed with two different mixtures; and the effect of time; temperature; and sample-to-solvent ratio on total phenolic content (TPC) was investigated. Data were fitted to a second-order polynomial model. Multiple regression analysis and analysis of variance were used to determine the fitness of the model and optimal conditions for TPC. Three-dimensional surface plots were generated from the mathematical models. TPC at optimal conditions was 280.25 ± 3.94 mg of Gallic Acid Equivalent (GAE) 100 g−1 dm upon extraction with aqueous methanol/acetone, and 236.37 ± 5.26 mg GAE 100 g−1 dm with aqueous ethanol mixture. The phenolic profile of extracts obtained at optimal conditions was also investigated by HPLC. The two extracting procedures did not show different specificities for phenolic compounds but differed in the extraction yield.

scholarly journals Optimization of the extraction of phenolic compounds and antioxidant activity from the roots of Waltheria ovata using the response surface methodology

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 2095-2102
Author(s):  
O. Herrera-Calderon ◽  
R. Vega
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Dpph Assay ◽  
Sample Ratio

Waltheria ovata is a medicinal plant belonging to the Sterculiaceae genus. Natural products of Waltheria ovata could be used in the food industry as natural antioxidants due to its high content of polyphenols according to the literature. The main objective in this research was to optimize the extraction of phenolic compounds and the antioxidant activity from Waltheria ovata roots using response surface methodology (RSM). The total phenolic content in different extracts was determined by spectrophotometric method (Folin-Ciocalteu reagent) and the antioxidant activity by using DPPH assay. To optimize the conditions for total phenolic content and antioxidant activity were used three independent variables: solvent/sample ratio (1:10, 1:20 and 1:30 g/mL), temperature (40, 50, and 60°C) and time (40, 50 and 60 mins). The results showed that total phenolic content and antioxidant activity in the experiments ranged from 8.7 to 12.1 mg GAE/g and 76.1% to 96.7%, respectively. The coefficients of determination (R2 values) for phenolic content and antioxidant activity were 0.86 and 0.91, respectively. Under the optimum conditions of 1:20 g/mL, 60°C and 55 mins of extraction, the values for total phenolic content and antioxidant activity were 0.448±0.02 mg GAE/g and 87.00±2.0%, respectively. These data showed that the experimental responses were reasonably close to the predicted responses (0.444 mg GAE/g and 84.67%). Therefore, the results showed that Waltheria ovata can be used as antioxidant in foods.

scholarly journals Determining the basic composition and total phenolic compounds of Pleurotus sajor-caju cultivated in three different substrates by solid state bioprocess

2012 ◽  
Vol 3 (2) ◽  
pp. 11-14 ◽  
Author(s):  
Marcelo Fossa Da Paz ◽  
Carlos Alexandre Breyer ◽  
Rafael Fontana Longhi ◽  
Manuel Salvador Vicente Plata Oviedo
Keyword(s):  
Phenolic Compounds ◽  
Crude Protein ◽  
Apple Pomace ◽  
Total Phenolic ◽  
Crude Protein Content ◽  
Total Phenolic Compounds ◽  
Basic Composition ◽  
Oyster Mushrooms ◽  
Pleurotus Sajor Caju ◽  
Cultivation Substrate

Oyster mushrooms (Pleurotus sajor-caju) were grown in grape bagasse, apple pomace or bean straw. Samples were taken for each one of the substrates and analyzed for moisture, ash, carbohydrate, crude fat, crude fiber, crude protein content and total phenolic compounds. The substrate affected all the parameter content and mainly the total phenolic compounds. In general, the nutritional aspects of mushrooms produced using grape bagasse tended to be better. Changes in the total phenolic compound content of the fruit could be explained by the biosorption capacity from substrate. It was possible to confirm that composition depends on cultivation substrate.

scholarly journals Optimization of extraction conditions for polyphenols from the stem bark of Funtumia elastica (Funtum) utilizing response surface methodology

2021 ◽  
Vol 4 ◽  
pp. 46
Author(s):  
Theophilus Fadjare Frempong ◽  
Nathaniel Owusu Boadi ◽  
Mercy Badu
Keyword(s):  
Phenolic Compounds ◽  
Response Surface ◽  
Response Surface Method ◽  
Phenolic Content ◽  
Stem Bark ◽  
Total Phenolic ◽  
Liquid Ratio ◽  
Surface Method ◽  
Solid Liquid ◽  
The Impact

Background: The recovery of phenolic compounds is seen as an arduous task because phenolic compounds are available as free aglycones, as sugar or ester conjugates, or as polymers with several monomeric components. Furthermore, phenolic compounds do not disperse evenly and may be connected to cell walls, carbohydrates, or proteins. This study looks at the optimization of factors that affect the efficiency for the extraction of phenolic compounds from the stem-bark of Funtumia elastica. Methods: Five independent variables (solvent concentration, time, the temperature, solid-liquid ratio, and pH) of the extraction process were selected. Single factor analysis as well as the response surface method was used to evaluate the impact of the selected factors on the total phenolic content. The effect of the extraction factors on the phenolic content was statistically significant (p <0.05). For the response surface method, a five/factor, five/level central composite design used, and a fitted second-order polynomial regression model equation was used to show how the extraction parameters affected the total phenolic recovery. Results: The predicted value (R² of 0.5917) agreed with the adjusted value (R² of 0.7707). The residuals for response predictions were less than 5%. The optimal factors for the extraction were ethanol concentration of 75.99% v/v, extraction time of 193.86 minutes, temperature of 63.66°C, pH of 5.62, and solid-liquid ratio of 1:21.12 g/mL. Actual overall content of the phenolic compounds was validated at 82.83 ± 3.335 mg gallic acid equivalent (GAE) /g weight of extract, which agreed with the predicted response of 89.467 mg GAE/g of the dried extract under the optimal factors. Conclusions: The rich phenolic content of stem-bark of Funtumia elastica points to its potential as a functional medicinal product to alleviate diseases caused by oxidative stress such as asthma, breathing disorders, inflammation, and cardiovascular diseases.

Sign in / Sign up

Export Citation Format

Share Document