scholarly journals Bacterial Load of the Teat Apex Skin and Associated Factors at Herd Level

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1647
Author(s):  
Maria-Franziska Hohmann ◽  
Nicole Wente ◽  
Yanchao Zhang ◽  
Volker Krömker
Keyword(s):  
Management Practices ◽  
Bacterial Load ◽  
Antimicrobial Treatment ◽  
Coliform Bacteria ◽  
Air Samples ◽  
Herd Level ◽  
Laboratory Procedures ◽  
Environmental Pathogens ◽  
One Year ◽  
Mastitis Pathogens

In order to reduce antimicrobial treatment and prevent environmental mastitis, the aim of the present study was to investigate associations between herd level factors and microbial load on teat ends with environmental mastitis pathogens. Quarterly farm visits of 31 dairy farms over a one-year period were used for statistical analysis. During each farm visit, teat-skin swabs, bedding and air samples were taken and management practices and herd parameters were documented. Total mesophilic bacteria, esculin-positive streptococci and coliform bacteria were examined in the laboratory procedures from teat skin and environmental samples. Esculin-positive streptococci and coliform bacteria on teat ends increased with high temperature–humidity indices (THI) in the barn during the spring and summer. Significantly more coliform bacteria on teat ends were found in herds with an increased percentage of normal or slightly rough teat ends. Cleaning cubicles more frequently, pre-cleaning teats before milking as well as post-dipping them after milking had a decreasing effect of teat-skin load with total mesophilic and coliform bacteria at the herd level. To conclude, teat-skin bacterial load with environmental pathogens is subject to fluctuations and can be influenced by aspects of farm hygiene.

scholarly journals Etiology of Bovine Mastitis

2021 ◽  
Author(s):  
Muhammad Shoaib ◽  
Amjad Islam Aqib ◽  
Muhammad Aamir Naseer ◽  
Zeeshan Ahmad Bhutta ◽  
Wanxia PU ◽  
...  
Keyword(s):  
Bovine Mastitis ◽  
Coliform Bacteria ◽  
Economic Losses ◽  
Published Data ◽  
Common Disease ◽  
Primary Concern ◽  
Dairy Farmers ◽  
Dairy Animals ◽  
Environmental Pathogens ◽  
Mastitis Pathogens

Mastitis in dairy animals is the primary concern of dairy farmers, which is the most common disease that causes huge economic losses in the dairy industry. The economic losses due to mastitis are from a reduction in milk yield, condemnation of milk with antibiotic residues, veterinary treatment costs, and death. In addition, some mastitis pathogens also cause serious human diseases associated with the contamination of milk or milk products with bacteria or their toxins. Bovine mastitis is mainly caused by a wide range of environmental and contagious bacterial mastitis pathogens. Contagious pathogens are those whose main reservoir is the infected udder. Contagious pathogens mainly spread among animals during milking process whereas environmental pathogens spread from environment to udder at any time. The source of the environmental pathogens is the surrounding environment of an animal. The major contagious pathogens include Staphylococcus aureus, Streptococcus agalactiae, and Mycoplasma spp. and the minor contagious pathogens include Corynebacterium bovis and others. Major environmental pathogens include coliform bacteria (Escherichia coli, Klebsiella spp., Enterobacter spp. and Citrobacter spp.), environmental streptococci (Strep. dysgalactiae, Strep. uberis). This chapter covers detailed review of published data on contagious and environmental pathogens responsible for bovine mastitis.

scholarly journals Are Semi-Quantitative Clinical Cultures Inadequate? Comparison to Quantitative Analysis of 1053 Bacterial Isolates from 350 Wounds

Diagnostics ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1239
Author(s):  
Thomas E. Serena ◽  
Philip G. Bowler ◽  
Gregory S. Schultz ◽  
Anna D’souza ◽  
Monique Y. Rennie
Keyword(s):  
Quantitative Analysis ◽  
Best Management Practices ◽  
Management Practices ◽  
Chronic Wounds ◽  
Point Of Care ◽  
Bacterial Load ◽  
Quantitative Culture ◽  
Simple Method ◽  
Point Of Care Technology ◽  
Care Technology

Early awareness and management of bacterial burden and biofilm is essential to wound healing. Semi-quantitative analysis of swab or biopsy samples is a relatively simple method for measuring wound microbial load. The accuracy of semi-quantitative culture analysis was compared to ‘gold standard’ quantitative culture analysis using 428 tissue biopsies from 350 chronic wounds. Semi-quantitative results, obtained by serial dilution of biopsy homogenates streaked onto culture plates divided into 4 quadrants representing occasional, light, moderate, and heavy growth, were compared to total bacterial load quantified as colony-forming units per gram (CFU/g). Light growth, typically considered an insignificant finding, averaged a clinically significant 2.5 × 105 CFU/g (SE = 6.3 × 104 CFU/g). Occasional growth (range: 102–106 CFU/g) and light growth (103–107 CFU/g) corresponded to quantitative values that spanned a 5-log range; moderate and heavy growth corresponded to a range of 4-log and 6-log, respectively, with a high degree of overlap in range of CFU/g per category. Since tissue biopsy and quantitative culture cannot be widely practiced and semi-quantitative analysis is unreliable, other clinically relevant approaches are required to determine wound bioburden and guide best management practices. Fluorescence imaging is a point-of-care technology that offers great potential in this field.

scholarly journals Tracking the Evolution of Riverbed Morphology on the Basis of UAV Photogrammetry

2021 ◽  
Vol 13 (4) ◽  
pp. 829
Author(s):  
Teresa Gracchi ◽  
Guglielmo Rossi ◽  
Carlo Tacconi Stefanelli ◽  
Luca Tanteri ◽  
Rolando Pozzani ◽  
...  
Keyword(s):  
Management Practices ◽  
Low Cost ◽  
3D Models ◽  
Vegetation Coverage ◽  
Detection Analysis ◽  
Digital Terrain ◽  
Fluvial Dynamics ◽  
Aerial Vehicle ◽  
One Year ◽  
Short Time

Unmanned aerial vehicle (UAV) photogrammetry has recently become a widespread technique to investigate and monitor the evolution of different types of natural processes. Fluvial geomorphology is one of such fields of application where UAV potentially assumes a key role, since it allows for overcoming the intrinsic limits of satellite and airborne-based optical imagery on one side, and in situ traditional investigations on the other. The main purpose of this paper was to obtain extensive products (digital terrain models (DTMs), orthophotos, and 3D models) in a short time, with low costs and at a high resolution, in order to verify the capability of this technique to analyze the active geomorphic processes on a 12 km long stretch of the French–Italian Roia River at both large and small scales. Two surveys, one year apart from each other, were carried out over the study area and a change detection analysis was performed on the basis of the comparison of the obtained DTMs to point out and characterize both the possible morphologic variations related to fluvial dynamics and modifications in vegetation coverage. The results highlight how the understanding of different fluvial processes may be improved by appropriately exploiting UAV-based products, which can thus represent a low-cost and non-invasive tool to crucially support decisionmakers involved in land management practices.

scholarly journals Prevalence and herd-level risk factors of bovine tuberculosis in the State of Goiás, Brazil

2016 ◽  
Vol 37 (5Supl2) ◽  
pp. 3625 ◽  
Author(s):  
Willian Vilela Rocha ◽  
Valéria De Sá Jayme ◽  
Ana Lourdes Arrais de Alencar Mota ◽  
Wilia Marta Elsner Diederichsen de Brito ◽  
Glauciane Ribeiro de Castro Pires ◽  
...  
Keyword(s):  
Risk Factors ◽  
Bovine Tuberculosis ◽  
Protective Factor ◽  
Management Practices ◽  
Multiple Logistic Regression Analysis ◽  
Livestock Production ◽  
The State ◽  
Economic Losses ◽  
Strong Concentration ◽  
Herd Level

Bovine tuberculosis results in economic losses in livestock production and poses a risk to public health. This study aimed to characterize the epidemiological status of bovine tuberculosis in adult cows in the state of Goiás, Brazil. The state was divided into three sampling strata, corresponding to different livestock production circuits. A total of 18,659 animals were tested with the comparative cervical tuberculin test (CCT), in 300 randomly sampled bovine herds per stratum. An epidemiological questionnaire was used to identify health and management practices that may be associated with the presence of tuberculosis infection in the herd. In Stratum 1, dominated by beef cattle, no animals reacted to the CCT. In Stratum 2, which covers the main dairy regions of the state, the prevalence was estimated at 8.67% [5.73 to 12.74%] for herds and at 0.9% [0.21 to 1.58%] for animals. In Stratum 3, characterized by a mix of dairy, beef and dual-purpose herds, the prevalence was estimated at 1.00% [0.21 to 2.89] for herds and 0.30% [0.10 to 0.49 %] for animals. The overall prevalence in the state of Goiás was 3.43% [2.20 to 4.67%] for herds and 0.30% [0.10 to 0.49%] for animals. The multiple logistic regression analysis revealed that herd-level risk factors associated with the presence of the bovine tuberculosis are: the location of the herd in Stratum 2 (OR = 12.05 [3.52 to 41.28]) and the number of times a cow is milked per day (OR = 6.27 [2.72 to 14.44]). Regular veterinary care was identified as a protective factor (OR = 0.38 [0.15-0.94]). These results indicate that bovine tuberculosis is endemic in the state; its spatial distribution is heterogeneous with a strong concentration in dairy regions. The most intensive dairy farms are those with the highest risk, which is consistent with what other authors’ found elsewhere in Brazil. The epidemiological information generated by this study provides information for planning of risk-based surveillance actions, and justifies the adoption of free-herd certification programs for bovine tuberculosis in the main dairy regions of Goiás.

scholarly journals Planting density and initial growth of two tree species adapted to the semi-arid region

2012 ◽  
Vol 36 (5) ◽  
pp. 951-960
Author(s):  
Paulo Sérgio Lima e Silva ◽  
Alexandre Emanuel Régis Holanda ◽  
Haroldo Nogueira de Paiva ◽  
Fábio Henrique Tavares de Oliveira ◽  
Odaci Fernandes de Oliveira
Keyword(s):  
Management Practices ◽  
Block Design ◽  
Gliricidia Sepium ◽  
Plant Age ◽  
Production Costs ◽  
Planting Density ◽  
Initial Growth ◽  
Randomized Block Design ◽  
Semi Arid Region ◽  
One Year

Planting densities influence several aspects of forest formation, including management practices, timber yield, quality, and extraction, and consequently its production costs. The objective of this study was to evaluate Mimosa caesalpiinifolia and Gliricidia sepium growth as a function of planting density (400, 600, 800, 1000, and 1200 plants ha-1) and plant age. The species were evaluated every 90 days for plant height (PH), crown diameter (CD) and root collar diameter (RCD) (10 cm above the ground), with the first evaluation performed at 90 days and the last at 720 days. When plants were one year of age and beyond, evaluations were conducted also for stem diameter at breast height (DBH) (1.30 m above the ground). A randomized block design with split-plots and three replicates was adopted. Species were assigned to plots, planting densities were assigned to subplots, and evaluation ages were assigned to subsubplots. The four traits in both species had their values decreased as planting density increased, but continually increased as plant age increased. For PH and RCD there was an alternation between species superiority, with gliricidia being superior to sabiá at some ages, while the opposite occurred at other ages. As to CD the species only differed in the last measurement, gliricidia being superior. With regard to DBH, gliricidia was superior starting from the second measurement. There was an effect of the species × ages interaction for the four traits and also an effect of the densities × ages interaction for CD and DBH.

Bacteriological and pathological investigations on the preputial glands of one-year-old C57BL/6NCrl mice maintained in individually ventilated cages

2021 ◽  
pp. 002367722110559
Author(s):  
Gianfranco Di Caro ◽  
Lucia Minoli ◽  
Marzia Ferrario ◽  
Gerardo Marsella ◽  
Gianpaolo Milite ◽  
...  
Keyword(s):  
Bacterial Load ◽  
Laboratory Animals ◽  
Inflammatory Cell Infiltrate ◽  
Preputial Gland ◽  
Inflammatory Score ◽  
Haematological Changes ◽  
Preputial Glands ◽  
One Year ◽  
Inflammatory Changes ◽  
Klebsiella Spp

Spontaneous infections of the preputial glands represent overlooked health problems in mice that could raise welfare concerns and potentially confound scientific experiments. Agents involved in preputial gland infections have rarely been investigated, with opportunistic pathogens of laboratory animals usually detected in inflamed preputial glands. The aim of this study was to investigate the prevalence of bacterial infection in the preputial glands and the relationship between haematological and pathological changes and infection status. We analysed 40 preputial glands from 20 one-year-old C57BL/6NCrl male mice by using bacteriology, haematology and pathology. Bacteria were isolated from 16/20 (80%) mice, for a total of 32/40 (80%) examined preputial glands. Enterobacter cloacae, Pasteurella spp., Klebsiella spp. and Staphylococcus aureus were identified in 35%, 17.5%, 15% and 12.5% of the examined glands, respectively. Preputial gland inflammation was identified in 29/40 (72.5%) glands and was classified as chronic interstitial adenitis in 27 cases and suppurative adenitis in the remaining two glands. No haematological changes were found in mice with infected glands. Histologically, the presence of intralesional bacteria, intraluminal necrotic material, intraluminal keratin accumulation, interstitial inflammatory cell infiltrate and granulocytes (intraluminal and/or interstitial), along with total inflammatory score and total histopathological score, were significantly increased in infected glands and correlated with the bacterial load. Most severe inflammatory changes were identified after S. aureus infection, while ductal hyperkeratosis was significantly increased in glands infected with Klebsiella spp. In conclusion, preputial gland infection was a common event in one-year-old C57BL/6NCrl mice, and bacterial load correlated with pathological findings, while systemic effects were not highlighted by haematology.

A ‘crimmigrant ban’? Global mobility, urban (in)security and the changing dynamics of judicial practices

2021 ◽  
pp. 146247452110478
Author(s):  
Eleonora Di Molfetta
Keyword(s):  
Crime Prevention ◽  
Management Practices ◽  
Public Spaces ◽  
Criminal Court ◽  
Future Research ◽  
Urban Management ◽  
Residence Permit ◽  
Global Mobility ◽  
One Year ◽  
Empirical Material

In the last decades, western countries have developed a set of policies and practices aimed both at crime prevention and social reassurance. Within this trend, the old-fashioned sanction of banishment has regained prominence. Banning orders, in particular, are widely used to remove from public spaces individuals who are deemed a threat to public safety and urban decorum. This article investigates the use of banning orders towards foreign defendants without a valid residence permit in an Italian criminal court. Based on empirical material collected during a one-year period of courtroom ethnography in Turin, this article sheds light on the rationales and objectives behind the use of banning orders. The interviews with courtroom actors reveal how banning orders have lost much of their preventive dimension to become an instrument of socio-urban control towards immigrants. This article invites future research to consider the role that urban management practices might play in the field of global mobility.

Microbiological quality and safety of Ready- to- eat foods from restaurants and food establishments in Yirgalem town Southern, Ethiopia

2019 ◽  
Author(s):  
Tsegaye Shamebo Arficho ◽  
Asefa Hamato Kebede
Keyword(s):  
Bacterial Load ◽  
Microbiological Quality ◽  
Food Samples ◽  
Coliform Bacteria ◽  
Plate Count ◽  
Southern Ethiopia ◽  
Microbiological Analysis ◽  
Quality And Safety ◽  
Mannitol Salt Agar ◽  
Microbiological Methods

Abstract Background: Foodborne illnesses are considered as one of the most important public health problems particularly in developing countries like Ethiopia. This study aimed to determine the microbiological quality and safety of ready-to-eat foods in Yirgalem town, southern Ethiopia from November 2016 to August 2017. Methods: The collection of ready-to-eat food samples and laboratory-based microbiological analysis was used as the study design. A total of 160 food samples comprising of 40 ‘Injera firfir’, 40‘Bayeaynet’, 40 Vegetables and 40 Spaghetti were collected and analyzed for microbial contamination following standard microbiological methods. Ten grams of each food sample was transferred into 90 ml of buffered peptone water and homogenized for 5 minutes using a vortex mixer. The homogenates were serial diluted up to 10-7 and a volume of 0.1ml aliquot was spread plated on pre-solidified media of Aerobic plate count agar, MacConkey agar, Mannitol salt agar, and Salmonella-Shigella agar and incubate at 35-37oc for 24 hrs. Also, Potato Dextrose Agar was used for the isolation of fungi. Data were entered into Microsoft Excel and analyzed using SPSS version 20.0. Results: All the collected food samples were subjected to total aerobic mesophilic bacteria, Coliform bacteria, Enterobacteriaceae, Staphylococcal, Yeasts, and Molds counts. Accordingly, the mean counts expressed as log10 CFU/g of food for each group of the organism were 7.90 ± 0.71, 4.31±1.30, 4.32 ± 1.30, 6.70 ± 0.34 and 4.5 ± 1.01, respectively. The highest bacterial load 162(28.9%) was detected in ‘Injera firfir’ whereas the lowest 108(19.2%) case was investigated in Spaghettis. Regarding the food safety issue, the frequency of S. aureus, E. coli and Salmonella spp in the food samples were 54.4%, 43.8%, and 0.6%, respectively. Conclusion: The high microbial load and existence of foodborne pathogens in ready-to-eat foods in Yirgalem town, Southern Ethiopia is calling for the creation of awareness among restaurant and food establishment owners and food handlers concerning the hygienic practice. Keyword: Microbial quality, Yirgalem town, Southern Ethiopia

scholarly journals Lolium multiflorum seeds in the soil: I. Soil seed bank dynamics in a no til system

2008 ◽  
Vol 30 (2) ◽  
pp. 100-110 ◽  
Author(s):  
Fernanda Costa Maia ◽  
Manoel de Souza Maia ◽  
Renée M. Bekker ◽  
Rogério Previatti Berton ◽  
Leandro Sebastião Caetano
Keyword(s):  
Seed Bank ◽  
Management Practices ◽  
Transition Period ◽  
Soil Seed Bank ◽  
Lolium Multiflorum ◽  
Storage Period ◽  
Annual Ryegrass ◽  
Seed Population ◽  
Seed Bank Dynamics ◽  
One Year

The objective of the study was to characterize annual ryegrass seed population dynamics, managed for natural re-sowing, in no til systems in rotation with soybean, in different chronosequences An area was cultivated for two years with soybean, left as fallow land for the next two years and then cultivated again with soybean for the next two years. The four chronosequences represented different management periods, two with soybean (6 and 8 years old) and the other two resting (3 and 9 years old). Soil samples were taken every month during one year and divided into two depths (0-5 and 5-10 cm). Vegetation dynamics were also evaluated (number of plants, inflorescences and seedlings). Soil seed bank (SSB) dynamics showed structural patterns in time, with a "storage period" in summer, an "exhausting period" during autumn and a "transition period" in winter and spring. Pasture establishment by natural re-sowing was totally dependent on the annual recruitment of seeds from the soil. The influence of the management practices on the SSB was more important than the number of years that these practices had been implemented. Places where soybean was sown showed the largest SSBs. Most of the seeds overcame dormancy and germinated at the end of the summer and beginning of the autumn, showing a typically transitory SSB, but with a small proportion of persistent seeds

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