scholarly journals Longitudinal Metabolic Biomarker Profile of Hyperketonemic Cows from Dry-Off to Peak Lactation and Identification of Prognostic Classifiers

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1353
Author(s):  
Anna Mareike Couperus ◽  
Fabian Schroeder ◽  
Peter Hettegger ◽  
Johann Huber ◽  
Thomas Wittek ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Metabolic Diseases ◽  
Transition Period ◽  
Early Stage ◽  
Post Partum ◽  
Area Under The Curve ◽  
Likelihood Method ◽  
Control Group ◽  
Key Factor ◽  
Peak Lactation

Currently about 30% to 50% of all dairy cows are affected by a metabolic or infectious disease during the transition period. A key factor for preventive actions is the ability to precisely predict metabolic diseases at an early stage. We report the longitudinal metabolic profile of non-esterified fatty acids, beta-hydroxybutyrate (BHB), total bilirubin, and aspartate aminotransferase in hyperketonemic dairy cows. Aiming for a novel measurement regime to improve metabolic health in dairy cows, we evaluated prognostic classifiers for hyperketonemia. In the observational longitudinal study, 99 healthy adult primiparous and multiparous Simmental dairy cows were included. Every cow was monitored weekly for 14 consecutive weeks, beginning two weeks prior to the expected day of parturition until peak lactation. Cows with serum concentrations of BHB > 0.8 mmol/L were considered hyperketonemic. Biomarker profiles were fitted by the maximum likelihood method using a mixed effects natural cubic spline model. In the hyperketonemic group, the BHB profile remained significantly higher than that of the control group until the end of the study period. As a prognostic classifier, the cut-off level of 0.54 mmol/L BHB measured on the 10th day post partum had the highest area under the curve. These results provide new longitudinal insights into the metabolic biomarker progression of dairy cows and enable an early onset diagnosis of hyperketonemia.

scholarly journals Effect of Pegbovigrastim on Hematological Profile of Simmental Dairy Cows during the Transition Period

Animals ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 841 ◽  
Author(s):  
Francesca Trimboli ◽  
Valeria Maria Morittu ◽  
Antonio Di Loria ◽  
Andrea Minuti ◽  
Antonella Anna Spina ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Blood Cells ◽  
Transition Period ◽  
Lower Percentage ◽  
Control Group ◽  
Volume Distribution ◽  
Polymorphonuclear Cells ◽  
Distribution Width ◽  
Hematological Profile ◽  
First Time

Pegbovigrastim is a long-acting analog of recombinant bovine granulocyte colony-stimulating factor, that promotes and increases the count and functionality of polymorphonuclear cells in dairy cows. The present study aimed to explore, for the first time in Simmental cows, the clinical and hematological effect of pegbovigrastim during the transition period (TP). Cows were randomly assigned into two groups: treated group (PEG; n = 16) received pegbovigrastim at approximately 7 days before expected parturition and within 6 h after calving, and control group (CTR; n = 16) received saline solution. Blood samples were obtained at −7, 0, 1, 3, 7, 14, 21, and 30 days relative to calving. PEG group showed white blood cells (WBC) count consistently higher compared with CTR group (p < 0.001) until to 3 weeks after calving. Neutrophils remained higher in PEG group (p < 0.001) up to three weeks after calving, compared with CTR group, with slight increment of band cells. Moreover, PEG group displayed a lower index of myeloperoxidase at 1, 3, and 7 days after calving (p < 0.01) compared with CTR. Basophils and lymphocytes showed a similar trend to those observed for neutrophils at 1 day after calving in PEG group. Finally, monocytes remained markedly elevated until 3 days after calving in PEG compared to CTR group (p < 0.001), whereas in PEG group, eosinophils population showed lower percentage values at 1 and 3 days after calving but higher values at 30 days compared with CTR group. PEG group was characterized by lower red blood cells (RBCs) count compared with CTR group (p < 0.05) and higher % of red cell volume distribution width (RDW) from week 2 and mean corpuscular volume (MCV) at 30 days after calving. In addition, the mean platelet volume (MPV) was significantly higher in PEG group at calving, 1, 3, and 7 days after calving compared with CTR group (p < 0.05). For the first time, we described the effect of pegbovigrastim in a breed not specialized exclusively in milk production as Holstein, but with dual purpose (meat and milk), evaluating the complete hematological profile in cows during the transition period. These results provide evidence on the proliferative effect of pegbovigrastim on WBC in Simmental breed highlighting its possible side effect on RBCs.

scholarly journals Relationship between expression pattern of vitamin D receptor, 1 alpha-hydroxylase enzyme, and chemokine RANTES genes and selected serum parameters during transition period in Holstein dairy cows

2020 ◽  
Vol 7 (1) ◽  
pp. e000339
Author(s):  
Hala A R Saed ◽  
Hussam M M Ibrahim ◽  
Sabry A El-Khodery ◽  
Mohamed A Youssef
Keyword(s):  
Gene Expression ◽  
Vitamin D ◽  
Dairy Cows ◽  
Transition Period ◽  
Post Partum ◽  
Serum Levels ◽  
Vdr Gene ◽  
Genetic Expression ◽  
Enzyme Gene ◽  
Holstein Dairy Cows

ObjectivesThe objective of the present study was to evaluate the pattern of genetic expression of vitamin D receptor (VDR), 1 alpha-hydroxylase (1α-OHase) enzyme and chemokine regulated on activation normal T-cell expressed and secreted (RANTES) in peripheral blood of Holstein dairy cows during transition period.MethodsBlood samples were collected from 16 Holstein dairy cows at 3 weeks prior expected date of delivery (EDD), at the day of parturition, and 3 weeks post-partum for assessment of expression profile of studied genes using real-time PCR and measurement of glucose, ionized calcium (Ca), parathyroid hormone (PTH), inorganic phosphorous (P), sodium (Na), potassium (K), chloride (Cl), and magnesium (Mg) levels.ResultsCompared with 3 weeks prior EDD, VDR gene expression decreased significantly at the day of parturition then increased significantly at 3 weeks post-partum. The genetic expression of 1α-OHase enzyme as well as PTH, K, Na and Cl levels increased significantly at the day of parturition. The Ca level decreased significantly at the day of parturition then increased significantly at 3 weeks post-partum. The P level increased significantly at the day of parturition then decreased significantly at 3 weeks post-partum. Glucose level decreased significantly at the day of parturition and at 3 weeks post-partum. RANTES gene expression showed non-significant changes among the three different time points. The expression of VDR gene had a negative correlation with the expression of 1α-OHase enzyme gene, and serum levels of glucose, PTH, P and K, but had a positive correlation with the serum Ca level. The expression of 1α-OHase enzyme gene had a positive correlation with serum levels of PTH, P and K, but had a negative correlation with the serum Ca level.ConclusionsResults of the current study indicate the importance of monitoring the genetic expression of VDR and 1α-OHase enzyme as indicators of metabolic changes during transition period, suggesting that they are candidate genes to judge the health status of dairy cows during such period.

Milk production, peripartal liver triglyceride concentration and plasma metabolites of dairy cows fed diets supplemented with calcium soaps or hydrogenated triglycerides of palm oil

2009 ◽  
Vol 77 (2) ◽  
pp. 151-158 ◽  
Author(s):  
Roland G Karcagi ◽  
Tibor Gaál ◽  
Piroska Ribiczey ◽  
Gyula Huszenicza ◽  
Ferenc Husvéth
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Yield ◽  
Palm Oil ◽  
Milk Fat ◽  
Post Partum ◽  
Negative Energy ◽  
Plasma Metabolites ◽  
Control Group ◽  
Liver Triglyceride

The aim of the study was to test the effect of rumen-inert fat supplements of different chemical forms or containing different unsaturated/saturated (U/S) fatty acid contents on milk production, milk composition and liver and blood metabolic variables of high-yielding dairy cows in the peripartal period. Thirty Holstein-Friesian dairy cows were divided into three equal groups and fed a corn silage-based diet, without fat supplementation (control) or supplemented with 11·75 MJ NEl per day of calcium soaps of palm oil fatty acids (CAS; U/S=61/39) or with 11·75 MJ NEl per day of hydrogenated palm oil triglyceride (HTG; U/S=6/94). Each diet was fed from 25±2 d prior to the expected calving to 100±5 d post partum. Compared with the control, both CAS and HTG supplementation resulted in an increase of the average milk yield. Milk fat content and fat-corrected milk yield were higher in the HTG group but lower in the CAS group than in the control group. In all groups liver triglyceride concentrations (TGL) increased from 15 d prepartum to 5 d post partum, and then decreased thereafter. At 5 d TGL was lower in the HTG group than control or CAS cows. No significant differences were detected in TGL among dietary treatments at 15 d prepartum and 25 d post partum. Higher plasma glucose and insulin and lower non-esterified fattay acids and β-hydroxybutyrate concentrations and aspartate aminotransferase activity were measured in the HTG group than in the control or CAS groups at 5 d or 25 d post partum. Our results show that HTG may provide a better energy supply for high-yielding dairy cows in negative energy balance than CAS around calving.

scholarly journals Effect of concentrate supplementation during transition period on production and reproduction of indigenous buffalo

2020 ◽  
Vol 49 (1) ◽  
pp. 83-90
Author(s):  
MR Habib ◽  
MZ Islam ◽  
MS Bari ◽  
MZ Islam ◽  
MAH Sarker ◽  
...  
Keyword(s):  
Milk Yield ◽  
Transition Period ◽  
Post Partum ◽  
Mustard Oil ◽  
Control Group ◽  
Common Salt ◽  
Concentrate Supplementation ◽  
Heat Period ◽  
Days Open ◽  
The Impact

This study was to investigate the impact of concentrate supplementation on the growth, milk yield and quality, postpartum heat period and days open in transient buffalo. Hence, six selected indigenous transient buffaloes were equally divided into two groups viz. control (n=3) and supplemented (n=3). About 56.0 kg mixed green fodders (Para:German=3:1) and 2.0 kg concentrate mixtures were offered as a basal diet to each buffalo. Besides these, an additional amount (0.5 kg) of concentrate mixture (wheat bran-50%, mustard oil cake-40%, common salt-2% and di-calcium phosphate-8%) was supplied to each buffalo of the supplemented group. The dry matter intake of the supplemented buffalo was 2.5 and 2% higher (p<0.001) at pre- and post-partum period, respectively than that of the control fed buffalo. Pre- and post-partum body weight, and body condition score of buffaloes between the group were found similar (p>0.05). About 20% more (p=0.02) milk was obtained in the supplemented group compared to the control group, however, the milk composition was not different (p>0.05). Calf birth weight was found ≈10% higher (p=0.51) in the supplemented group than that of the control group. Postpartum heat period and days open of the supplemented buffaloes were reduced remarkably by 13 and 14 days, respectively compared to the buffaloes in control group. Overall, concentrate supplementation to the transient indigenous buffaloes has noteworthy effects on milk yield, postpartum heat period and days open. Bang. J. Anim. Sci. 2020. 49 (1): 83-90

scholarly journals The effect of Glycoline® on reproductive efficiency in high-producing dairy cows

2016 ◽  
pp. 5163-5176
Author(s):  
Marcelo R. Del Campo ◽  
Marco Gonzalez T ◽  
Hans Andresen ◽  
Freddy A. Cea
Keyword(s):  
Dairy Cows ◽  
Pregnancy Rate ◽  
Transition Period ◽  
Holstein Cows ◽  
Reproductive Efficiency ◽  
Control Group ◽  
Ovarian Activity ◽  
Uterine Infection ◽  
Uterine Involution ◽  
Downer Cow Syndrome

ABSTRACT Objective. Determine the effect of Glycoline® on reproductive efficiency in high producing dairy cows. Materials and methods. 100 Holstein cows one month before delivery were selected. About 21 days before parturition they were put in a barn and were randomized in two groups: Glycoline® Group (GG, n=50), 300 g/day of Glycoline® for 21 days antepartum and 250 g/day of GlycoLine® over the following 21 days postpartum, and the Control Group (CG, n=50) with the same feed and silage ration as GG during the same period, but without the addition of Glycoline®. Events and reproductive variables of the cows were recorded for 202 days. The data were systematized, analyzed and statistically compared. Results. Comparisons were made between GG and CG respectively: Retained placenta (0.0 vs. 12.0%; p=0.027), downer cow syndrome (14.3 vs. 44.0%; p=0.002), uterine involution (64.6 vs. 36.4%; p=0.019 ), uterine infection (10.4% vs. 35.5%; p=0.006), no ovarian activity (6.3 vs. 25.6%; p=0.018), follicular cysts (0.0 vs. 18.2%, p = 0.002), luteal structures (25.0 vs. 9.3% ; p=0.058), mean estrus presentation (40.1% vs. 63.5%; p=0.033) inseminated females (79.6 vs. 68.0%; p=0.017), pregnancy rate at day 150 (57.1 vs. 46.0%; p≥0.317 ) and intervals (days): calving to 1st estrus (39.8 vs. 63.2; p≤0.006), calving to IA (62.4 vs. 87.5; p≤0.006) calving to conception (81.7 vs. 93.6; p≤0.006; p=0.103). Conclusions. Results suggest that dietary Glycoline® added in the transition period improves reproductive efficiency of high-producing dairy cows. RESUMEN Objetivos. Determinar el efecto del Glycoline® sobre la eficiencia reproductiva en vacas lecheras de alta producción. Materiales y Métodos. Se seleccionaron 100 vacas Holstein un mes antes del parto. Alrededor de 21 días antes del parto se ubicaron en un establo y se asignaron al azar a dos Grupos: Grupo Glycoline® (GG; n=50), 300 g/día de Glycoline® durante 21 días preparto y 250 g/día de Glycoline® durante los siguientes 21 días posparto y Grupo Control (GC, n=50) la misma ración de concentrado y ensilaje que el GG durante el mismo período de tiempo, pero sin la adición de Glycoline®. Se registraron los eventos y las variables reproductivas de las vacas durante 202 días. Los datos fueron sistematizados, analizados y comparados estadísticamente. Resultados. Comparaciones entre el GG y GC respectivamente: Retención de placenta (0,0 vs 12.0%; p=0.027), síndrome de vaca caída (14.3 vs 44.0%; p=0.002), involución uterina (64.6 vs 36.4%; p=0.019), infección uterina (10.4% vs 35.5%; p=0.006), sin actividad ovárica (6.3 vs 25.6%; p=0.018), quistes foliculares (0.0 vs 18.2%, p=0.002), estructuras luteales (25.0 vs 9.3%; p=0.058), promedio presentación de celo (40.1% vs 63.5%; p=0.033), hembras inseminadas (79.6 vs 68.0%; p=0.017), tasa de preñez al día 150 (57.1 vs 46.0%; p≥0.317) y intervalos de tiempo (días): parto 1er -celo (39.8 vs 63.2; p≤0.006), parto-IA (62.4 vs 87.5; p≤0.006) y parto-preñez (81.7 vs 93.6; p≤0.006; p=0.103). Conclusiones. Los resultados sugieren que el Glycoline® adicionado en la dieta en el período de transición, mejora la eficiencia reproductiva de vacas de alta producción.

scholarly journals Peripartal Rumen-Protected L-Carnitine Manipulates the Productive and Blood Metabolic Responses in High-Producing Holstein Dairy Cows

2021 ◽  
Vol 8 ◽  
Author(s):  
Mohsen Danesh Mesgaran ◽  
Hassan Kargar ◽  
Sadjad Danesh Mesgaran ◽  
Ali Javadmanesh
Keyword(s):  
Palmitic Acid ◽  
Dairy Cows ◽  
Milk Fat ◽  
Post Partum ◽  
Energy Utilization ◽  
Utilization Efficiency ◽  
Metabolic Responses ◽  
Control Group ◽  
Lactation Period ◽  
Holstein Dairy Cows

This study aimed to monitor the effect of including rumen-protected L-carnitine (Carneon 20 Rumin-Pro, Kaesler Nutrition GmbH, Cuxhaven, Germany) in the transition diet on the productive and metabolic responses of multiparous high-producing Holstein dairy cows. Thirty-two multiparous cows were allocated in a completely randomized design to receive the same diet plus 60 g fat prill containing 85% palmitic acid (control, n = 16) or 100 g rumen-protected L-carnitine (RLC, n = 16); at 28 days before expected calving until 28 days in milk (DIM). Fat prill was included in the control diet to balance the palmitic acid content of both experimental diets. Milk production over the 28 DIM for the control and RLC groups was 46.5 and 47.7 kg, respectively. Milk fat content tended to increase upon rumen-protected L-carnitine inclusion (p = 0.1). Cows fed rumen-protected L-carnitine had higher fat- and energy-corrected milk compared with the control group. Pre- and post-partum administration of L-carnitine decreased both high- and low-density lipoprotein concentrations in peripheral blood of post-partum cows. The results of this study indicated that the concentration of triglycerides and beta-hydroxybutyrate was not significantly different between the groups, whereas the blood non-esterified fatty acid concentration was markedly decreased in cows supplemented with L-carnitine. Animals in the RLC group had a significant (p &lt; 0.05) lower blood haptoglobin concentration at 7 and 14 DIM than the control. Animals in the RLC group had a lower concentration of blood enzymes than those of the control group. The mRNA abundance of Toll-like receptors 4, cluster of differentiation 14, and myeloid differential protein 2 did not significantly change upon the supplementation of L-carnitine in the transition diet. In summary, the dietary inclusion of RLC improved dairy cow's performance during the early lactation period. Greater production, at least in part, is driven by improved energy utilization efficiency and enhanced metabolic status in animals during the periparturient period.

scholarly journals The Transition Period Updated: A Review of the New Insights into the Adaptation of Dairy Cows to the New Lactation

Dairy ◽  
2021 ◽  
Vol 2 (4) ◽  
pp. 617-636
Author(s):  
Matteo Mezzetti ◽  
Luca Cattaneo ◽  
Matilde Maria Passamonti ◽  
Vincenzo Lopreiato ◽  
Andrea Minuti ◽  
...  
Keyword(s):  
Immune System ◽  
Dairy Cows ◽  
Metabolic Disorders ◽  
Metabolic Diseases ◽  
Transition Period ◽  
Time Frame ◽  
Early Lactation ◽  
Immune Functions ◽  
Balanced Diet ◽  
Immune Dysfunctions

Recent research on the transition period (TP) of dairy cows has highlighted the pivotal role of immune function in affecting the severity of metabolic challenges the animals face when approaching calving. This suggests that the immune system may play a role in the etiology of metabolic diseases occurring in early lactation. Several studies have indicated that the roots of immune dysfunctions could sink way before the “classical” TP (e.g., 3 weeks before and 3 weeks after calving), extending the time frame deemed as “risky” for the development of early lactation disorders at the period around the dry-off. Several distressing events occurring during the TP (i.e., dietary changes, heat stress) can boost the severity of pre-existing immune dysfunctions and metabolic changes that physiologically affect this phase of the lactation cycle, further increasing the likelihood of developing diseases. Based on this background, several operational and nutritional strategies could be adopted to minimize the detrimental effects of immune dysfunctions on the adaptation of dairy cows to the new lactation. A suitable environment (i.e., optimal welfare) and a balanced diet (which guarantees optimal nutrient partitioning to improve immune functions in cow and calf) are key aspects to consider when aiming to minimize TP challenges at the herd level. Furthermore, several prognostic behavioral and physiological indicators could help in identifying subjects that are more likely to undergo a “bad transition”, allowing prompt intervention through specific modulatory treatments. Recent genomic advances in understanding the linkage between metabolic disorders and the genotype of dairy cows suggest that genetic breeding programs aimed at improving dairy cows’ adaptation to the new lactation challenges (i.e., through increasing immune system efficiency or resilience against metabolic disorders) could be expected in the future. Despite these encouraging steps forward in understanding the physiological mechanisms driving metabolic responses of dairy cows during their transition to calving, it is evident that these processes still require further investigation, and that the TP—likely extended from dry-off—continues to be “the final frontier” for research in dairy sciences.

scholarly journals REDUCTION OF THE VOLUNTARY WAITING PERIOD BY USE OF HORMONAL PROTOCOLS AND FIXED-TIME ARTIFICIAL INSEMINATION (FTAI) IN DAIRY COWS

2012 ◽  
Vol 17 (3) ◽  
Author(s):  
Miriane Suplicy ◽  
Haroldo Suplicy Filho ◽  
Luiz Ernandes Kozicki ◽  
José Carlos dos Santos Breda ◽  
Romildo Romualdo Weiss ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Pregnancy Rate ◽  
Artificial Insemination ◽  
Post Partum ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Control Group ◽  
Waiting Period ◽  
Equine Chorionic Gonadotropin ◽  
Voluntary Waiting Period

ABSTRACT: The aim of this study was to reduce the waiting voluntary period in dairy cows by administration of two different hormonal protocols for estrus synchronization and fixed-time artificial insemination (FTAI). One hundred twenty-eight Holstein dairy cows were studied. Three groups (G) were carried out: G1 (44 treated), G2 (34 treated) and G3 (50 as control). In the treated cows the following hormonal protocols was applied between 40 and 45 days post partum: G1- Day 0 (D0) - bovine intravaginal device containing progesterone (P4) plus 1.0 mg of estradiol benzoate (EB), D8 - removal of the P4, plus 0.5 mg of cloprostenol plus 400 IU of equine chorionic gonadotropin (eCG), D9 - 1.0 mg of EB and FTAI after 36 hours. G2 - D0 - P4 intravaginal plus 50 mcg of gonadotropin-releasing hormone (GnRH), D7 - removal of the P4 plus cloprostenol plus 400 IU eCG; D9 - 50 mcg of GnRH and FTAI 16 hours after. G3 – Control group inseminated 12 hours after the visual estrus. Results: G1 and G2 were inseminated (FTAI) respectively in the D55.5 and D56.8 after calving, while the G3 in the D84.5 (p<0.01).  The pregnancy rate in the G1, G2 and G3 was respectively 47.0, 46.1 and 35.8% (p>0.05).  In Conclusion: there was significant reduction in the voluntary waiting period in treated cows and higher pregnancy rate although not significant.

157 EFFECTS OF METABOLIC CONDITIONS OF DAIRY COWS DURING TRANSITION PERIOD AND EARLY LACTATION IN WINTER AND SUMMER ON HEALTH AND FERTILITY

2015 ◽  
Vol 27 (1) ◽  
pp. 169
Author(s):  
M. Maturana Filho ◽  
K. M. Lemes ◽  
J. R. Naves ◽  
T. Santin ◽  
T. K. Nishimura ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Dairy Cows ◽  
Transition Period ◽  
Disease Incidence ◽  
Animal Health ◽  
Group V ◽  
Retained Placenta ◽  
Metabolic Conditions ◽  
The Difference ◽  
Peak Lactation

The aim of this study was to investigate and determine which metabolic predictors measured during the transition period, have a better association with health and fertility parameters in dairy cows in winter (W) and summer (S).The experiment was conducted with 235 multiparous Holstein dairy cows. The animals were divided, retrospectively into the experimental groups [High Production (AP), ≥45.9 to 65 kg of milk during peak lactation; Medium production (MP), between 30 and 45.8 kg of milk during peak lactation]. Blood samples and evaluations occurred during the transition period and during the timed fixed artificial insemination (TAI). Data were analysed for the main effects of group, day, and their interaction using the PROC MIXED procedure of SAS software (SAS 9.3, SAS Institute, Inc., Cary, NC, USA). Creatine kinase (CK) plasmatic concentrations increased during peak lactation in all groups, but the difference was not significant. Variations in superoxide dismutase (SOD) concentrations were observed in HP cows in both seasons (HPW and HPS). These cows had higher levels of superoxide dismutase (SOD) when compared of MP cows (MPW and MPS), with significant changes since 7 days before calving and remained higher until 104 days of lactation. The differences were observed in lipid profile, particularly in NEFA concentration. For this variable, there were group (P < 0.001) and day (P < 0.001) effects and also double interactions (day v. season, P < 0.001) and triple interactions (group v. day v. season, P = 0.009). The HPS cows had higher values, as well as a variation pattern of urea concentration (P < 0.05), different from the other groups until 100 days in milk (DEL). No differences were observed in diseases incidences between group or periods (P > 0.05). There was a statistical tendency to all groups in the summer season for earlier calving (P = 0.1), less calf weight (P = 0.07), and retained placenta (P = 0.09) and metritis incidence (P = 0.08). These parameters were strongly correlated with metritis disease incidence and the significant parameters: Earlier calving (P = 0.03), dystocia (P = 0.05), and retained placenta (P = 0.009). The NEFA (0.4 mmol L–1) and BHBA concentrations (0.7 mmol L–1) were highly correlated with the occurrence of uterine diseases. We observed high levels of AST (around 128 U/L) pre calving in ketotic and in cows with displacement of abomasum. According to these results, we concluded that milk production was not a risk factor for fertility in the first three services. Also, metabolic changes in the transition period were determinant in milk yield and health diseases. Research was supported by FAPESP, CNPq, CAPES, Fazenda Colorado, and Ourofino animal health.

A new complementary approach to endoscopy for esophageal cancer diagnosis.

2017 ◽  
Vol 35 (4_suppl) ◽  
pp. 42-42
Author(s):  
Gengxi Jiang ◽  
Hongmei Tao ◽  
Xing Tang ◽  
Da Lou ◽  
Yue Lin ◽  
...  
Keyword(s):  
Esophageal Cancer ◽  
Cancer Diagnosis ◽  
Early Stage ◽  
Area Under The Curve ◽  
Squamous Carcinoma ◽  
Pathological Examination ◽  
Control Group ◽  
Threshold Values ◽  
Blood Samples ◽  
Therapeutic Decision Making

42 Background: For esophageal cancer, no viable non-invasive detection technologies are available today. In earlier investigations, Cancer Differentiation Analysis (CDA) Technology which measures information relating to both protein fragments and cellular signals in blood samples in a single test has showed a significant advantage in esophageal cancer diagnosis. Methods: Blood samples from 105 individuals in EDTA tubes were collected from Shanghai Changhai Hospital, China, between July and December 2014, CDA and endoscopy tests were carried out on all samples before clinically diagnosed. A performance predication model of CDA and endoscopy test results was built using pROC package in R Language for the data of Area Under the Curve (AUC) and CDA threshold values. Further analysis was carried out based on the CDA threshold values. Results: Out of the 105 individuals, samples from 6 individuals diagnosed as esophageal benign diseases were selected as control group. 99 samples from individuals diagnosed as esophageal cancer, of which 3 and 82 cases were adenocarcinoma and squamous carcinoma, 2, 26, 33, 31 and 7 cases were classified as stage 0,I,II, III, IV, respectively. Details of AUC results of endoscopy, and CDA plus endoscopy for each group were given in Table 1 below. Conclusions: Even though the sensitivity of the endoscopy is usually high due to its pathological examination, this investigation showed that CDA technology could further enhance the diagnosis sensitivity of endoscope, especially for early stage of esophageal cancer, and it (CDA) can be an effective complementary tool to clinical diagnosis and operation determination while obtaining the pathological information via endoscopy. In conclusion, CDA technology is of great value in esophageal diagnosis and therapeutic decision-making. [Table: see text]

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