scholarly journals The Effect of L-Glutamine on Basal Albumen and Yolk Indices and Albumen Amino Acids Composition

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3556
Author(s):  
Ewa Tomaszewska ◽  
Anna Arczewska-Włosek ◽  
Artur Burmańczuk ◽  
Renata Pyz-Łukasik ◽  
Janine Donaldson ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Lipid Content ◽  
Egg Quality ◽  
Egg Yolk ◽  
Experimental Period ◽  
Cholesterol Content ◽  
Amino Acid Profile ◽  
Amino Acids Composition ◽  
Yolk Cholesterol

The current study tested the hypothesis that 1.0% dietary inclusion of L-glutamine (Gln), an non-essential amino acid that influences protein synthesis, can improve internal egg quality, including amino acids profile. Thirty-week-old Bovans Brown laying hens in their middle laying period were assigned to one of the two experimental groups (12 replicate cages, 2 hens/cage) with Gln in the form of alpha-ketoglutarate (10 g/kg) or without Gln inclusion. The experimental period lasted for 30 wks, from the 31st to the 60th week of age of hens, when eggs were collected and selected egg quality indices were determined. Gln supplementation had no effect on albumen and egg yolk share, albumen and yolk basal indices and composition, including yolk cholesterol content. However, Gln decreased the lipid content of the egg albumen (p < 0.001), and influenced albumen amino acid profile, increasing content of asparagine (p < 0.05), phenylalanine (p < 0.05), proline (p < 0.001), tryptophan (p < 0.01), and tyrosine (p < 0.05). In conclusion, the study shows a potential role of Gln supplementation for enhancing nutritional values of eggs by lower lipid content and higher amino acid profile.

scholarly journals Proximate Composition and Amino Acid Profile of the Edible Muscle of European Crayfish Astacus astacus L. Inhabiting the Orchomenos Region in Central Greece

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Papachristou E ◽  
◽  
Tyrpenou AE ◽  
Kastritsi-Katharios I ◽  
Kotzamanis Y ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Muscle Tissue ◽  
Lipid Content ◽  
Proximate Composition ◽  
Amino Acid Profile ◽  
Sea Bream ◽  
Astacus Astacus ◽  
Crude Lipid ◽  
Central Greece

The aim of this study was to determine the proximate composition and amino acid profile of the edible muscle of European crayfish Astacus astacus L. Animals were collected from Orchomenos region in Central Greece and muscle tissue samples were collected for chemical analysis. Total crude protein and crude lipid content of muscle tissues were determined using the Kjeldahl method and Folch’s procedure, respectively. Amino acids profile was performed by Ultra Performance Liquid Chromatographic (UPLC) determination of the acid hydrolysed muscle extract after derivatization with AccQ-Tag reagent (Waters, USA). The results showed that protein and lipid content of crayfish muscle tissue were 16.55 ± 0.4 g 100 g-1 and 0.52 ± 0.20 g 100 g-1, respectively. Also, the moisture and ash content were 80.93 ± 0.36 g 100 g-1 and 1.25 ± 0.14 g 100 g-1, respectively. Amino acids analysis of muscle tissue revealed that the highest values were those of glutamic acid (2.98 g 100 g-1) and arginine (2.19 g 100g-1) as well as those for aspartic acid (1.88 g 100 g-1), lysine (1.47 g 100 g-1) and leucine (1.33 g 100 g-1). The lowest values were found for histidine (0.35 ± 0.03 g 100 g-1) and methionine (0.47 ± 0.01 g 100 g-1). The results of this study showed that the crayfish of Orchomenos region can be considered as an important source of essential nutrients in the human diet due to its protein and essential amino acids content, which are relatively comparable to those of farmed sea bream (Sparus aurata L.), as well as to farmed and wild sea bass (Dicentrarchus labrax). However, the lipid content of Orchomenos crayfish was found to be insignificant and negligible. Overall, the protein quality of European crayfish can be considered as a main factor in selecting this species as a candidate species for European aquaculture diversification.

scholarly journals Effect of Alternative Housing System and Hen Genotype on Egg Quality Characteristics

2018 ◽  
pp. 695 ◽  
Author(s):  
Zofia Sokołowicz ◽  
Józefa Krawczyk ◽  
Magdalena Dykiel
Keyword(s):  
Rhode Island ◽  
Egg Quality ◽  
Egg Yolk ◽  
Shape Index ◽  
Cholesterol Content ◽  
Housing System ◽  
Shell Weight ◽  
Alternative Housing ◽  
Egg Yolks ◽  
Yolk Cholesterol

The aim of the study was to determine the effect of type of alternative housing system and layer genotype on the quality of table eggs. The investigated eggs came from native Greenleg Partridge hens (Z-11) and Rhode Island Red hens (R-11) covered by the gene-pool protection program, as well as from Hy-line Brown commercial hens from litter barn (LS), free range (FR), and organic systems (OS). Eggs for the study were collected from the hens at 56 wk of age. Egg quality assessment was based on the following values egg traits: weight, shape index, yolk, albumen, and shell percentage in the whole egg; eggshell traits: color intensity, weight, thickness, density, breaking strength, physical features of egg content: albumen height, value of Haugh units, yolk color; presence of meat/ blood spots and cholesterol, higher fatty acids, vitamin A and E in egg yolks. In the rearing systems under study, eggs with the highest weight were laid by commercial hens, and the type of alternative rearing system had no significant effect on this trait. Shell colour intensity depends on the breed of hen, whereas the rearing system has no significant effect on this trait. Hen genotype and housing system had an effect on egg shell weight, thickness, and density. The housing system influences the content of MUFA, PUFA, PUFAn-6 and PUFAn-3 as well as PUFA 6/3 in egg yolks. No effect of the housing system on egg yolk cholesterol content was observed.

scholarly journals Egg Quality Parameters, Production Performance and Immunity of Laying Hens Supplemented with Plant Extracts

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 975
Author(s):  
Muhammad Ammar Dilawar ◽  
Hong Seok Mun ◽  
Dhanushka Rathnayake ◽  
Eun Ju Yang ◽  
Ye Seul Seo ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Proximate Composition ◽  
Laying Hens ◽  
Egg Quality ◽  
Egg Yolk ◽  
Cholesterol Content ◽  
Production Performance ◽  
Dependent Manner ◽  
Yolk Cholesterol ◽  
Dietary Treatments

This study examined the effects of Mentha arvensis (MA) and Geranium thunbergii (GT) extracts in drinking water on the production performance, egg quality, cholesterol content of egg yolk, proximate composition, and sensory qualities of egg and immunity parameters in laying hens. Ninety-six 28-week-old Hy-Line Brown layers were randomly divided into four dietary treatments for 16 weeks. The dietary treatments were (1) control, (2) T1 (0.01% 1 MA:1 GT), (3) T2 (0.05% 1 MA:1 GT), and (4) T3 (0.1% 1 MA:1 GT). Egg production increased significantly with increasing levels of MA and GT. The egg weight was increased in T2, and the feed intake was highest in T2 and T3 (p < 0.05). The Haugh unit and egg shape index were significantly better in T3 and the control than with other treatments (p < 0.05). The content of yolk cholesterol was significantly lower (p < 0.05) in T2 and T3. On the other hand, there were no significant differences in the egg proximate composition. A significant increase in the serum interleukin 6 (IL-6), tumor necrosis factor (TNFα) and immunoglobulins (IgG and IgA) concentration was observed in the birds fed plant extracts when compared to the control. On average, T2 and T3 showed significantly lower (p < 0.05) concentrations of NH3 gas from the feces as compared to the control. This study suggests that MA and GT supplementation could improve the laying performance, egg quality, and immunity, and decrease the egg yolk cholesterol content in a dose-dependent manner.

scholarly journals Egg quality and yolk lipid composition of laying hens fed diets containing cashew nut meal

2013 ◽  
Vol 33 (1) ◽  
pp. 172-179 ◽  
Author(s):  
Tatiana Fontoura Vidal ◽  
Ana Lúcia Fernandes Pereira ◽  
Virgínia Kelly Gonçalves Abreu ◽  
Ednardo Rodrigues Freitas ◽  
Manoel Alves Sousa Neto ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Egg Quality ◽  
Total Lipid Content ◽  
Egg Yolk ◽  
Cholesterol Content ◽  
Laying Hen ◽  
Total Solids ◽  
Fatty Acids Profile ◽  
Cashew Nut ◽  
Yolk Cholesterol

The objective of this study was to assess the effect of the addition of cashew nuts meal (0, 5, 10, 15, 20, and 25%) to laying hen diets on egg quality and yolk composition. The variables studied were: egg weight, specific gravity, Haugh Units, percentages of shell, albumen, and yolk, moisture, total solids, total lipids, fatty acids profile, and yolk cholesterol. The addition of up to 25% of cashew nuts meal to hen diets did not affect egg quality and freshness, moisture and total solids content. However, an increase in total lipid content and a decrease in yolk pigmentation was observed. Oleic acid level increased in the yolk, whereas palmitic, stearic, and linoleic acid levels decreased. The addition of cashew nuts meal increased the monounsaturated/saturated fatty acid ratio in the yolk and reduced the cholesterol content. Therefore, the use of cashew nuts meal in laying hen diets favorably modifies the fatty acid composition of egg yolk and contributes to a better acceptance of this food by consumers since it also reduces yolk cholesterol levels.

scholarly journals Food supplements from a Grasshopper: A developmental stage-wise evaluation of amino acid profile, protein and vitamins in Brachystola magna (Girard)

2019 ◽  
pp. 561 ◽  
Author(s):  
P.B. Zamudio-Flores ◽  
M. Hernández -Gonzaléz ◽  
V.G. García- Cano
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Content ◽  
Adult Stage ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Food Supplement ◽  
Water Soluble ◽  
Stage Of Development ◽  
Amino Acids Composition

Insects can be used as an easily accessible food supplement; however, for nutritional purposes it is necessary to consider its protein content, amino acids composition and vitamin content. In this study a nutritional characterization (protein content, amino acid profile and hydrosoluble and liposoluble vitamins) was performed to the Brachystola magna (Girard) grasshopper in four stages of its biological cycle (egg, nymph 3, nymph 4 and adult). In the adult stage the highest values of protein content (> 59%) were observed compared with the other stages of development. At all stages of B. magna the presence of 9 out of the 10 essential amino acids were detected (just tryptophan amino acid was absent). Significant increases in the fat-soluble vitamins (A, D and E) were observed as the stage of development of B. magna increased, with the increase of vitamin E of ≈ 662% in the adult stage; however, the water-soluble vitamins of the B complex remained constant at all stages, but vitamin C, which increased significantly in the adult stage of this insect cycle. Results suggest that Brachystola magna (Girard) could be a potential source of consumption for people with specific health needs.

Plasma-Free Amino Acid Profiling of Nasal Polyposis Patients

2020 ◽  
Vol 22 (9) ◽  
pp. 657-662 ◽  
Author(s):  
Mustafa Celik ◽  
Alper Şen ◽  
İsmail Koyuncu ◽  
Ataman Gönel
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acid ◽  
Free Amino ◽  
Nasal Polyposis ◽  
Amino Acid Profile ◽  
Healthy Control Group ◽  
Control Group ◽  
Healthy Control ◽  
History Of

Aim and Objective:: To determine the mechanisms present in the etiopathogenesis of nasal polyposis. It is not clear whether amino acids contribute in a causal way to the development of the disease. Therefore, the aim of this study was to determine the plasma-free amino acid profile in patients with nasal polyposis and to compare the results with a healthy control group. Materials and Methods:: This was a prospective controlled study that took place in the Otolaryngology Department at the Harran University Faculty of Medicine between April 2017 and April 2018. Plasmafree amino acid profile levels were studied in serum samples taken from a patient group and a healthy control group. Patients who were diagnosed with bilateral diffuse nasal polyposis and were scheduled for surgical interventions were included in this study. Individuals whose age, gender, and body mass index values were compatible with that of the patient group and who did not have any health problems were included in the control group. All the participants whose levels of plasma-free amino acid were thought to be affected by one or more of the following factors were excluded from the study: smoking and alcohol use, allergic rhinitis presence, the presence of acute or chronic sinusitis, a history of endoscopic sinus surgery, unilateral nasal masses, a history of chronic drug use, systemic or topical steroid use in the last three months for any reason, and liver, kidney, hematological, cardiovascular, metabolic, neurological, or psychiatric disorders or malignancies. Results: In patients with nasal polyposis, 3-methyl histidine (3-MHIS: nasal polyposis group (ng) = 3.22 (1.92 – 6.07); control group (cg) = 1.21 (0.77 – 1.68); p = 0.001); arginine (arg: ng = 98.95 (70.81 – 117.75); cg = 75.10 (54.49 – 79.88); p = 0.005); asparagine (asn: ng = 79.84 (57.50 – 101.44); cg = 60.66 (46.39 – 74.62); p = 0.021); citrulline (cit: ng = 51.83 (43.81 – 59.78); cg = 38.33 (27.81 – 53.73); p = 0.038); cystine (cys: ng = 4.29 (2.43 – 6.66); cg = 2.41 (1.51 – 4.16); p = 0.019); glutamic acid (glu: ng = 234.86 (128.75 – 286.66); cg = 152.37 (122.51 – 188.34); p = 0.045); histidine (his: ng = 94.19 (79.34 – 113.99); cg = 74.80 (62.76 – 98.91); p = 0.018); lysine (lys: ng = 297.22 (206.55 – 371.25); cg = 179.50 (151.58 – 238.02); p = 0.001); ornithine (ng = 160.62 (128.36 – 189.32); cg = 115.91 (97.03 – 159.91); p = 0.019); serine (ser: ng = 195.15 (151.58 – 253.07); cg = 83.07 (67.44 – 92.44); p = 0.001); taurine (tau: ng = 74.69 (47.00 – 112.13); cg = 53.14 (33.57 – 67.31); p = 0.006); tryptophan (trp: ng = 52.31 (33.81 – 80.11); cg = 34.44 (25.94 – 43.07); p = 0.005), homocitrulline (ng = 1.75 (1.27 – 2.59); cg = 0.00 (0.00 – 0.53); p = 0.001); norvaline (ng = 6.90 (5.61 – 9.18); cg = 4.93 (3.74 – 7.13); p = 0.021); argininosuccinic acid (ng = 14.33 (10.06 – 25.65); cg = 12.22 (5.77 – 16.87) p = 0.046); and plasma concentrations were significantly higher than in the healthy control group (p <0.05). However, the gamma-aminobutyric acid (gaba: ng = 0.16 (0.10 – 0.24); cg = 0.21 (0.19 – 0.29); p = 0.010) plasma concentration was significantly lower in the nasal polyposis group than in the healthy control group. Conclusion: In this study, plasma levels of 15 free amino acids were significantly higher in the nasal polyposis group than in the healthy control group. A plasma level of 1 free amino acid was found to be significantly lower in the nasal polyposis group compared to the healthy control group. Therefore, it is important to determine the possibility of using the information obtained to prevent the recurrence of the condition and to develop effective treatment strategies. This study may be a milestone for studies of this subject. However, this study needs to be confirmed by further studies conducted in a larger series.

scholarly journals Basil as Secondary Crop in Cascade Hydroponics: Exploring Salinity Tolerance Limits in Terms of Growth, Amino Acid Profile, and Nutrient Composition

Horticulturae ◽  
2021 ◽  
Vol 7 (8) ◽  
pp. 203
Author(s):  
Denisa Avdouli ◽  
Johannes F. J. Max ◽  
Nikolaos Katsoulas ◽  
Efi Levizou
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Salinity Tolerance ◽  
Total Concentration ◽  
Amino Acid Profile ◽  
Total Amino Acid ◽  
Cascade System ◽  
Hydroponic System ◽  
Salinity Levels ◽  
Use Efficiency

In a cascade hydroponic system, the used nutrient solution drained from a primary crop is directed to a secondary crop, enhancing resource-use efficiency while minimizing waste. Nevertheless, the inevitably increased EC of the drainage solution requires salinity-tolerant crops. The present study explored the salinity-tolerance thresholds of basil to evaluate its potential use as a secondary crop in a cascade system. Two distinct but complemented approaches were used; the first experiment examined basil response to increased levels of salinity (5, 10 and 15 dS m−1, compared with 2 dS m−1 of control) to identify the limits, and the second experiment employed a cascade system with cucumber as a primary crop to monitor basil responses to the drainage solution of 3.2 dS m−1. Growth, ascorbate content, nutrient concentration, and total amino acid concentration and profile were determined in both experiments. Various aspects of basil growth and biochemical performance collectively indicated the 5 dS m−1 salinity level as the upper limit/threshold of tolerance to stress. Higher salinity levels considerably suppressed fresh weight production, though the total concentration of amino acids showed a sevenfold increase under 15 dS m−1 and 4.5-fold under 5 and 10 dS m−1 compared to the control. The performance of basil in the cascade system was subject to a compromise between a reduction of fresh produce and an increase of total amino acids and ascorbate content. This outcome indicated that basil performed well under the conditions and the system employed in the present study, and might be a good candidate for use as a secondary crop in cascade-hydroponics systems.

scholarly journals Ion Chromatography Based Urine Amino Acid Profiling Applied for Diagnosis of Gastric Cancer

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Jing Fan ◽  
Jing Hong ◽  
Jun-Duo Hu ◽  
Jin-Lian Chen
Keyword(s):  
Gastric Cancer ◽  
Amino Acids ◽  
Amino Acid ◽  
Advanced Gastric Cancer ◽  
Free Amino Acid ◽  
Cancer Patients ◽  
Free Amino ◽  
Amino Acid Profile ◽  
Healthy Adults ◽  
Group D

Aim. Amino acid metabolism in cancer patients differs from that in healthy people. In the study, we performed urine-free amino acid profile of gastric cancer at different stages and health subjects to explore potential biomarkers for diagnosing or screening gastric cancer.Methods. Forty three urine samples were collected from inpatients and healthy adults who were divided into 4 groups. Healthy adults were in group A (n=15), early gastric cancer inpatients in group B (n=7), and advanced gastric cancer inpatients in group C (n=16); in addition, two healthy adults and three advanced gastric cancer inpatients were in group D (n=5) to test models. We performed urine amino acids profile of each group by applying ion chromatography (IC) technique and analyzed urine amino acids according to chromatogram of amino acids standard solution. The data we obtained were processed with statistical analysis. A diagnostic model was constructed to discriminate gastric cancer from healthy individuals and another diagnostic model for clinical staging by principal component analysis. Differentiation performance was validated by the area under the curve (AUC) of receiver-operating characteristic (ROC) curves.Results. The urine-free amino acid profile of gastric cancer patients changed to a certain degree compared with that of healthy adults. Compared with healthy adult group, the levels of valine, isoleucine, and leucine increased (P<0.05), but the levels of histidine and methionine decreased (P<0.05), and aspartate decreased significantly (P<0.01). The urine amino acid profile was also different between early and advanced gastric cancer groups. Compared with early gastric cancer, the levels of isoleucine and valine decreased in advanced gastric cancer (P<0.05). A diagnosis model constructed for gastric cancer with AUC value of 0.936 tested by group D showed that 4 samples could coincide with it. Another diagnosis model for clinical staging with an AUC value of 0.902 tested by 3 advanced gastric cancer inpatients of group D showed that all could coincide with the model.Conclusions. The noticeable differences of urine-free amino acid profiles between gastric cancer patients and healthy adults indicate that such amino acids as valine, isoleucine, leucine, methionine, histidine and aspartate are important metabolites in cell multiplication and gene expression during tumor growth and metastatic process. The study suggests that urine-free amino acid profiling is of potential value for screening or diagnosing gastric cancer.

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