scholarly journals Protein Source and Intake Effects on Diet Digestibility and N Excretion in Horses—A Risk of Environmental N Load of Horses

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3568
Author(s):  
Markku Saastamoinen ◽  
Susanna Särkijärvi ◽  
Heli Suomala
Keyword(s):  
Latin Square ◽  
Experimental Period ◽  
Rapeseed Meal ◽  
The Other ◽  
Protein Source ◽  
N Leaching ◽  
Faecal Excretion ◽  
Protein Supplements ◽  
Work Level ◽  
Supplemental Protein

Six Finnhorse mares were used in a digestibility trial, in which six typical horse diets were compared. The diets were: (A) haylage 100%; (B) hay 100%; (C) hay 70% and oats 30%; (D) hay 70% and soybean meal + oats 30%; (E) hay 70%, rapeseed meal + oats 30% and (F) hay 70 %, linseed meal + oats 30%. The trial was conducted according to an unbalanced 6 × 4 Latin square design with four 3-week experimental periods. The experimental period consisted of 17-day preliminary feeding which was followed by a 4-day total faecal and urine collection periods to evaluate N excretion. The digestibilities of DM (p < 0.001) and OM (p < 0.001) in the haylage-only diet were lower compared to the other diets. The supplemental protein feeds improved the diet digestibility of CP (p = 0.002) compared to a hay + oats diet. Furthermore, the DM (p = 0.019), OM (p = 0.006), and CP (p = 0.016) digestibilities of the soya-supplemented diet were better than those of the rapeseed- and linseed-supplemented diets. Faecal excretion was greater for haylage (19.3 kg fresh faeces and 3.6 kg DM/day) and hay (18.7 kg fresh faeces and 3.6 kg DM/day) diets (p < 0.001) compared with the other diets. Urine excretion was also greater for forage-only diets compared with diets including protein supplements. Horses excreted 14.0 L urine/day on haylage-only diet (p = 0.026) and 14.3 L/day on a hay-only diet (p = 0.003). Horses excreted more nitrogen in their urine than in dung. N excretion differed between the diets. Horses on a haylage-only diet excreted 51.6 g N in faeces /day and on hay-only diet 51.4 g N/day. On the other hand, when protein content in forages increased, N excretion via urine increased (haylage vs. dried hay). Horses excreted less N in urine with hay-only diet than with haylage-only or protein-supplemented diets (p < 0.001). When N excreted in faeces and urine was counted together, horses excreted less N with a hay-only diet (p < 0.001) than with a supplemented one (oats and/or protein feeds). The results showed that feed choices affected the amount of nitrogen excreted. Feeding recommendations should consider not only the horse category and work level, but also the protein source. When good quality protein is fed, smaller N intakes can be applied to reduce the N excretion via urine and dung. At the farm level, improved understanding of feed quality, as well as feeding planning and practices, is a way to decrease the risk of N leaching and evaporation.

scholarly journals Use of rapeseed and pea grain protein supplements for organic milk production

1999 ◽  
Vol 8 (3) ◽  
pp. 239-252 ◽  
Author(s):  
H. KHALILI ◽  
E. KUUSELA ◽  
E. SAARISALO
Keyword(s):  
Milk Yield ◽  
Dry Matter ◽  
Latin Square ◽  
Red Clover ◽  
Rapeseed Meal ◽  
Protein Supplements ◽  
Rapeseed Cake ◽  
The Third ◽  
Pisum Sativum L ◽  
Cold Pressed

Grass-red clover silage was fed ad libitum. In experiment 1 a duplicated 4 x 4 Latin square design was used. A mixture of oats and barley was given at 8 kg (C). Three isonitrogenous protein supplements were a commercial rapeseed meal (218 g kg-1 dry matter (DM); RSM), crushed organic field pea (Pisum sativum L.) (452 g kg-1 DM; P) and a mixture of pea (321 g kg-1 DM) and organic rapeseed (Spring turnip rape, Brassica rapa L. oleifera subv. annua) (155 g kg-1 DM; PRS). Cows on P and PRS diets produced as much milk as cows on the RSM diet. Milk yield was higher but protein content lower with PRS diet than with diet P. In experiment 2 a triplicated 3 x 3 Latin square design was used. A mixture of oats (395 g kg-1 ), barley (395 g kg-1 ) and a commercial heat-moisture treated rapeseed cake (210 g kg-1 ) was given at 8 kg (RSC). The second diet (ORSC) consisted (g kg-1) of oats (375), barley (375) and cold-pressed organic rapeseed cake (250). The third diet (RSCO) consisted (g kg-1) of oat (395), barley (395) and commercial heat-moisture treated rapeseed cake (250) and additional rapeseed oil (0.38 kg) to balance fat content between ORSC and RSCO diets. There was no dietary effect on the yield of energy corrected milk. Milk yield was higher with RSCO diet compared with other diets.;

The influence of type and timing of protein supplementation on wool growth and protein synthesis in the skin of young Merino sheep

1999 ◽  
Vol 50 (4) ◽  
pp. 497 ◽  
Author(s):  
D. G. Masters ◽  
G. Mata ◽  
S. M. Liu
Keyword(s):  
Protein Synthesis ◽  
Experimental Period ◽  
Protein Supplementation ◽  
The Other ◽  
Canola Meal ◽  
Protein Supplements ◽  
Wool Growth ◽  
Low Concentrations ◽  
High Concentrations ◽  
Dietary Treatments

There is limited evidence that the response in wool growth resulting from feeding protected protein supplements continues after the feeding has stopped. Feeding such proteins, alternated with traditional supplements, may increase wool growth as much as continuous feeding but at a lower cost. This experiment aimed to determine whether the response to protected protein continued after the sheep were switched to a cereal supplement. Over a 2-month experimental period, 56 weaners (5 months old, weighing 26 kg) were used in a 2 × 2 factorial experiment. Half were fed a diet containing 25% canola meal [partially protected protein with high concentrations of sulfur amino acids (SAA)] mixed with oaten hay, urea, and minerals. The other half were fed the same diet but with lupin seed (highly degradable protein with low concentrations of SAA) replacing the canola meal. Within each of the 2 dietary treatments and in each of 2 months, half of the weaners were fed the diet continuously, the other half were fed the diet for 2 weeks followed by 2 weeks of a barley, oats, hay, urea, and minerals diet. Another group of 8 weaners was fed the oats–barley diet continuously for 2 months. All sheep were fed to lose 35 g liveweight/day. Weaners fed canola meal grew 11% more wool during the experiment and had a higher rate of protein synthesis in the skin than weaners fed lupins. The response to canola meal of wool and skin was the same whether feeding was continuous or alternated with oats–barley, indicating that the benefits from feeding partially protected proteins continues after feeding has stopped.

Double-low rapeseed meal for pigs: ileal apparent digestibility of amino acids in diets containing various proportions of rapeseed meal, fish meal and soya-bean meal

1987 ◽  
Vol 44 (3) ◽  
pp. 415-420 ◽  
Author(s):  
I. G. Partridge ◽  
A. G. Low ◽  
J. J. Matte
Keyword(s):  
Amino Acids ◽  
Fish Meal ◽  
Latin Square ◽  
Rapeseed Meal ◽  
Soya Bean ◽  
Growing Pigs ◽  
Main Trial ◽  
Protein Supplements ◽  
Bean Meal ◽  
Soya Bean Meal

ABSTRACTIn order to determine the relative nutritional value of rapeseed meal (var. Tandem) in comparison with soya-bean meal and fish meal, seven barley-based diets were formulated to provide 13·1 MJ digestible energy and 8·8 g total lysine per kg. They contained soya-bean meal (S), rapeseed meal (R) or fish meal (F) alone, or equal proportions (on a total nitrogen basis) of soya and rapeseed, soya and fish meal, rapeseed and fish meal or soya, rapeseed and fish meal. The study involved 14 pigs growing from 40 to 90 kg, each fitted with a T cannula in the terminal ileum. Dietary treatments were applied during 7-day periods according to a 7 × 7 Latin-square design, replicated twice. The pigs were fed at 12-h intervals. Ileal digesta were collected during two 12-h periods from each pig after adaptation to each diet. Nutrient apparent digestibilities were measured using chromium III oxide as a marker; for diets S, R and F, respectively, values were: dry matter, 0·58, 0·51 and 0·66 (s.e. 0·014); N, 0·72, 0·67 and 0·73 (s.e. 0·011); lysine, 0·79, 0·72 and 0·83 (s.e. 0·010), with a similar relationship between diets for most other amino acids. In general, values for diets containing combinations of the protein supplements were intermediate between those for diets containing the respective single supplements. Following the main trial, seven pigs were given a semi-purified diet containing rapeseed meal as the only protein source; this gave amino acid digestibility values similar to or slightly higher than diet R. Diet R, which contained 375 g rapeseed meal per kg, presented no palatability problem. Rapeseed meal of the quality used in this trial could contribute a substantial proportion of the protein supplement for growing pigs. Its value should be enhanced by formulating diets according to the ileal digestibility of the limiting amino acids.

scholarly journals Digestibility and nitrogen balance of pig diets containing gradually increasing levels of rapeseed meal

2018 ◽  
Vol 68 (4) ◽  
pp. 521
Author(s):  
A KARGOPOULOS ◽  
V DOTAS ◽  
I NIKOLAKAKIS ◽  
D DOTAS
Keyword(s):  
Gradual Increase ◽  
Control Diet ◽  
Latin Square ◽  
Basal Diet ◽  
Experimental Period ◽  
Rapeseed Meal ◽  
Apparent Digestibility ◽  
Large White ◽  
Apparent Digestibility Coefficient ◽  
Fattening Pigs

Rapeseed meal (RSM), a by-product of extracted rapeseed oil production, is a potential protein source for use in pig diets. To determine the best levels of RSM inclusion in diets of fattening pigs as well as the digestibility and energy value of diets containing gradually increasing levels of RSM, a digestibility trial was conducted. The trial was performed according to a 4x4 Latin square experimental design, with 4 Large White x Landrace castrated male pigs weighing on average 47.3kg. The animals were housed in metabolism cages during the whole period the duration of which was a total of 66 days. The experiment was divided into 5 periods, an adjustment period and 4 experimental periods, during which pigs consumed 4 experimental diets. The diet C (control) was a basal diet based on corn and soybean meal, while the other experimental diets included RSM at levels 90 (low- L), 180 (medium- M) and 270 (high- H) g.kg-1 of feed, respectively. Each experimental period consisted of 10 preliminary days during which the animals were adapted to the diets, followed by a 4 days period for collection of faeces, urine and feed refusal. The gradual increase in the level of RSM in the experimental diets resulted in a significant (P<0.05) reduction in the apparent digestibility of CP, but only for the diet with the highest level of RSM, compared to the control diet and this one with the lowest level of RSM . Any increase in the level of RSM in the experimental diets resulted in significant (P<0.05) increase on the apparent digestibility coefficient (ADC) of CF and very significant (P<0.01) increase in diets M and H, compared to the control and diet L. Similar were the effects of RSM levels in the diets on the apparent digestibility coefficient of NDF, ADF and cellulose. As conclusion, the gradual increase in the level of RSM in the fattening pigs’ diets did not significantly affect ADC of DM and gross energy, caused a significant reduction in the ADC of CP, and a significant improvement in the ADC of CF as well.

scholarly journals The effects of a supplement of dietary fibre on faecal excretion by human subjects

1979 ◽  
Vol 41 (3) ◽  
pp. 495-503 ◽  
Author(s):  
Celia J. Prynne ◽  
D. A. T. Southgate
Keyword(s):  
Dietary Fibre ◽  
Considerable Increase ◽  
Human Subjects ◽  
Control Period ◽  
Experimental Period ◽  
The Other ◽  
Apparent Digestibility ◽  
Faecal Excretion ◽  
Gross Energy ◽  
Faecal Weight

1. Four human subjects on strictly controlled diets were given a fibre supplement, 25 g Ispaghula husk (Isogel)/d, for 3 weeks.2. Replicate diets and faeces were collected during two 5 d balance periods. The first period served as control for the second which occurred after the supplement had been fed for 2 weeks.3. Diets and faeces were analysed for total solids, gross energy, total nitrogen, fat, available and unavailable carbohydrates.4. Three of the four subjects showed a considerable increase in faecal bulk; total faecal weight was more than doubled in two subjects. In two subjects the increase was brought about mainly by extra fibre in the faeces accompanied by a higher proporation of faecal water whereas in one subject it was also an increased excretion of nutrients. One subject showed very little change in faecal bulk.5. High values for the apparent digestibility of fibre were found during the control period; from 0.70 to 0.80. There was more variation during the experimental period when apparent digestibility of total fibre ranged from 0.47 to 0.82. Values arrived at for the minimum apparent digestibility of the fibre supplement were generally high; one subject appeared to digest Isogel completely. Isogel was degraded preferentially to the food-derived fibre; in particular, cellulose in the faeces was increased during the experimental period.6. Only one subject showed distinct decreases in the apparent digestibility of energy, N and fat in the diet. The results do not therefore agree with the generally held view that increased fibre in the diet decreases the apparent digestibility of the other nutrients.

scholarly journals A comparison of untreated and formaldehyde-treated barley distiller’s solubles and rapeseed meal as protein supplements in dairy cows given grass silage ad libitum

1991 ◽  
Vol 63 (5) ◽  
pp. 455-463
Author(s):  
Pekka Huhtanen ◽  
Hannele Khalili ◽  
Matti Näsi
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Control Diet ◽  
Latin Square ◽  
Rapeseed Meal ◽  
Milk Composition ◽  
Protein Supplementation ◽  
Protein Supplements ◽  
Grass Silage ◽  
Ad Libitum

Twenty Friesian cows in four pens were arranged in a 4 x 4 Latin square experiment with 4 wk experimental periods to evaluate the effects on feed intake, milk yield and milk composition of treating barley dried distiller’s solubles (DDS) with a formaldehyde reagent, and to compare DDS with rapeseed meal as a protein supplement in dairy cows given a grass silage-based diet. The control diet (C) consisted of grass silage ad libitum fed with 8.5 kg/d of concentrate based on barley, oats and barley fibre (250, 250 and 500 g/kg dry matter (DM)). In three other diets 1.5 kg/d of the basal concentrate was replaced with rapeseed meal (RSM) treated for reduced ruminal degradability, 1.5 kg of untreated DDS (UDDS) or 1.5 kg of DDS treated with formaldehyde reagent at the level of 15 l/t (TDDS). On average, protein supplementation increased silage and total DM intake by approximately 0.5 kg/d (P>0.05). Milk yield (P

Guanidination procedure increases standardised ileal digestibilities of nitrogen and amino acids in rapeseed meal, soybean meal and peas fed to growing pigs

2013 ◽  
Vol 53 (3) ◽  
pp. 265 ◽  
Author(s):  
M. Eklund ◽  
W. R. Caine ◽  
W. C. Sauer ◽  
G. S. Huang ◽  
G. Diebold ◽  
...  
Keyword(s):  
Amino Acids ◽  
Soybean Meal ◽  
Crude Protein ◽  
Latin Square ◽  
Experimental Period ◽  
Rapeseed Meal ◽  
Antinutritional Factors ◽  
Growing Pigs ◽  
Equal Weight ◽  
Feed Ingredient

A digestibility study was conducted to assess the effect of using the guanidination procedure to convert lysine to homoarginine in dietary sources of protein on standardised ileal digestibilities of nitrogen and amino acids in rapeseed meal, soybean meal or peas fed to growing pigs. Six barrows (German Landrace × Pietrain) with an initial bodyweight of 19 ± 1.8 kg, fitted with ileal T-cannulas, were fed one of three cornstarch-based diets that contained 180 g crude protein/kg (as-fed basis) from rapeseed meal, soybean meal or peas for an experimental period of 10 days according to a double 3 by 3 Latin square design. On Day 9 of each experimental period, the pigs were fed diets containing the guanidinated protein sources, which corresponded to their respective unguanidinated diets. In the guanidinated assay diets, 50% of the dietary source of protein was replaced, on an equal weight basis, with the guanidinated assay feed ingredient. Using the guanidination procedure increased the content of nitrogen in rapeseed meal, soybean meal, and peas by 17, 34, and 8 g/kg DM, respectively. With the exception of lysine in rapeseed meal, using the guanidination procedure increased the standardised ileal digestibilities of most amino acids in rapeseed meal, soybean meal and peas (P < 0.05). Lower standardised ileal digestibililty of lysine in guanidinated compared with unguanidinated rapeseed meal indicates that guanidination was not as uniform compared with the other sources of dietary protein. In conclusion, increases in standardised ileal digestibilities of amino acids after using the guanidination procedure in the aforementioned feed ingredients may be due to solubilisation of protein and degradation of antinutritional factors such as fibre, trypsin inhibitors and tannins.

EFFECTS OF LYSINE INFUSION PER ABOMASUM OF STEERS FED CONTINUOUSLY

1972 ◽  
Vol 52 (4) ◽  
pp. 681-687 ◽  
Author(s):  
R. J. BOILA ◽  
T. J. DEVLIN
Keyword(s):  
Amino Acid ◽  
Crude Protein ◽  
Dry Matter ◽  
Latin Square ◽  
Linear Increase ◽  
The Body ◽  
The Other ◽  
Body Tissue ◽  
Rumen Microorganisms

Four dairy steers were allotted to four lysine infusion levels in a 4 × 4 latin square design and fed an 11.5% crude protein (90% dry matter (DM)) diet continuously (10-min intervals every 24 hr). Lysine hydrochloride equivalent to 0.0, 3.0, 6.0, and 9.0 g lysine per day was infused per abomasum. When 9 g lysine were infused per day, the percent of absorbed nitrogen (N) retained was significantly (P < 0.05) reduced; urinary N excretion as a percentage of N intake and plasma-free lysine were increased significantly compared with the other three infusion treatments. The infusion of 9 g lysine per day apparently exceeded the body tissue requirements for this amino acid and the excess N was excreted in the urine. A possibility of lysine being limiting (0.28% lysine of a 100% DM diet) was apparently offset by the synthesis of lysine by rumen microorganisms, which increased the dietary lysine two- to threefold. Increased levels of infused lysine did not result in a linear increase of lysine in the abomasum. With 3 g per day lysine infusion rumen ammonia and N retentions were high. However, a smaller amount of N reached the abomasum with steers on this treatment.

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