scholarly journals Influence of Age on the Standardized Ileal Amino Acid Digestibility of Corn and Barley in Broilers

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3575
Author(s):  
Mukti Barua ◽  
M. Reza Abdollahi ◽  
Faegheh Zaefarian ◽  
Timothy J. Wester ◽  
C. K. Girish ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Direct Method ◽  
Age Effect ◽  
Ileal Digestibility ◽  
Feed Formulation ◽  
Different Ages ◽  
Age Appropriate

The aim of this study was to determine the standardized ileal digestibility coefficients (SIDCs) of nitrogen (N) and amino acids (AAs) in corn and barley at six different ages (days 7, 14, 21, 28, 35 and 42) of broilers using the direct method. The apparent AA digestibility coefficients were corrected using age-appropriate basal endogenous AA losses. No age effect (p > 0.05) was noted for the SIDC of N in corn. The average SIDC of indispensable AAs (IAAs) and total AAs (TAAs) was influenced in a quadratic manner (p < 0.05) with the values being higher at day 7 that decreased at day 14, increased and plateaued between days 21 and 35 and dropped again at day 42. The average SIDC of dispensable AAs (DAAs) was influenced linearly (p < 0.05). In barley, the SIDC of N and average IAAs, DAAs and TAAs was affected (quadratic; p < 0.001) by age. The digestibility increased from day 7 to 21 and then plateaued up to day 42. The present findings confirm that the SIDC of AA in corn and barley are influenced by broiler age and that the age effect on AA digestibility may need to be considered for precise feed formulation.

PSVI-13 Amino Acid Supplementation During the Adaptation Period Did Not Affect the Standardized Ileal Digestibility of Amino Acids in Corn and Soybean Meal Fed to Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 200-201
Author(s):  
Hyunjun Choi ◽  
Sun Jong You ◽  
Beob Gyun G Kim
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Soybean Meal ◽  
Latin Square ◽  
Sole Source ◽  
Adaptation Period ◽  
Amino Acid Supplementation ◽  
Ileal Digestibility ◽  
Indispensable Amino Acids ◽  
Dietary Treatments

Abstract The objective was to determine the influence of amino acid (AA) supplementation during the adaptation period on the ileal digestibility of crude protein and AA in corn and soybean meal (SBM). Six barrows with an initial body weight of 30.9 ± 2.6 kg fitted with a T-cannula in the distal ileum were assigned to a 6 × 6 Latin square design with 6 dietary treatments and 6 periods. Two experimental diets contained corn or SBM as the sole source of AA and an N-free diet was additionally prepared. For AA supplementation groups, an AA mixture consisted of Gly, Lys, Met, Thr, Trp, Ile, Val, His, and Phe was added to the corn diet and the N-free diet at the expense of cornstarch, and an AA mixture of Lys, Met, and Thr was added to the SBM diet. All diets contained 0.5% of chromic oxide. The 6 experimental diets were fed to the pigs for 4 and half days, and the 3 diets containing AA mixture were switched to the respective diets without AA mixture during the following 2 and half days. Ileal digesta were collected during the last 2 days. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Arg and Trp in corn (P &lt; 0.05), but did not affect that in SBM. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Pro and Gly regardless of feed ingredient (P &lt; 0.05), but did not affect that of other AA. All AA except Pro in corn and SBM were unaffected by the addition of AA mixture during the adaptation period. In conclusion, the addition of amino acid during the adaptation period does not affect the standardized ileal digestibility of indispensable amino acids in feed ingredients.

scholarly journals Apparent ileal digestibility of amino acids and metabolisable energy value in grains for broilers

2008 ◽  
Vol 14 (4) ◽  
pp. 325 ◽  
Author(s):  
S. PERTTILÄ ◽  
J. VALAJA ◽  
T. JALAVA
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Broiler Chickens ◽  
Crude Protein ◽  
Dry Matter ◽  
Nitrogen Excretion ◽  
Ileal Digestibility ◽  
Metabolisable Energy ◽  
Feed Protein ◽  
Digestible Amino Acids

Using ileal digestible amino acids in feed optimising will intensify feed protein utilizing and decrease nitrogen excretion to the environment. The study determined the apparent ileal digestibility (AID) coefficients of amino acids in barley, wheat, oats, triticale, maize, and dehulled oats in the diets of 180 Ross broiler chickens (aged 24–35 days). The birds were fed semi-purified diets that contained grain as the sole protein source and chromium-mordanted straw as an indigestible marker. The AID coefficients of the nutrients were assessed using the slaughter technique, and the apparent metabolisable energy (AME) was determined using total excreta collection. The ileal digestibility of the dry matter and organic matter were the highest in maize. The AME of maize was higher than that of other cereals. The ileal digestibility of crude protein was higher in wheat than that in barley, oats and dehulled oats. The AME of wheat was similar to that of barley and oats but lower than that of triticale and dehulled oats. The amino acid AID was highest in wheat (0.86) and triticale (0.85) and lowest in oats (0.79) and barley 0.77). The average amino acid AID was 0.81 in dehulled oats. The threonine AID was the same in all tested ingredients. The lysine, methionine, and cystine AID coefficients were 0.81, 0.79, and 0.71 respectively for barley; 0.86, 0.84, and 0.38 respectively for oats; 0.87, 0.86, and 0.53 respectively for dehulled oats; 0.84, 0.90, and 0.66 respectively for maize; 0.89, 0.88, and 0.77 respectively for triticale; and 0.87, 0.85, and 0.71 respectively for wheat. Results indicated that AME –values of domestic grains (barley, oats and wheat) are in the same level. Especially, low AME value of wheat needs further investigation.;

scholarly journals 129 Effect of Ronozyme® WX supplementation on apparent and true ileal amino acid digestibility coefficients in broiler chicks fed varying levels of rice bran

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 112-112
Author(s):  
Richard B Omidiwura ◽  
Adebisi F Agboola ◽  
Taiwo O Makinde ◽  
Grace O Oyebode
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Rice Bran ◽  
Block Design ◽  
Interactive Effect ◽  
Essential Amino Acids ◽  
Broiler Chicks ◽  
Ileal Digestibility ◽  
Randomized Complete Block Design ◽  
Regression Approach

Abstract An experiment was conducted to investigate the effect of rice bran (RB) and Ronozyme® WX on apparent and true ileal amino acid (AA) digestibility coefficients in broiler chicks using a regression approach. Two hundred and forty 21-day-old Abor Acre plus chicks were randomly allotted to six diets with varying levels of RB (100, 200 and 300 g/kg) without (0g/kg) or with Ronozyme® WX (25g/kg) supplementation in a 2x3 factorial arrangement using a randomized complete block design. Each treatment had 5 replicates with 8 birds each. The birds were fed for 5 days (d 22–26). On d 26, digesta was sampled from distal ileum. Data were analyzed using general linear model at α0.05. Lysine had the highest concentration among the essential amino acids (AAs) while the lowest concentration was observed for threonine. Rice bran at 10% for the essential amino acids with enzyme was significantly higher but compared with concentrations of RB at 10% and 20% without enzyme. There were no significant differences among the true ileal amino acid digestibility coefficients except lysine (10% RB) without enzyme (0.91) which was significantly higher than 20% RB with enzyme (0.84) but similar to other treatments. Rice bran with enzyme had a significant (P &lt; 0.05) interactive effect on the apparent ileal digestibility coefficients of arginine, histidine, isoleucine methionine and phenylalanine. When RB was supplemented with enzyme, a significant (P &lt; 0.05) interactive effect on true ileal digestibility coefficients of arginine and methionine was observed. Rice bran-enzyme interaction had no significant effect on the non-essential AAs. The slopes of the regression lines ranged from 0.77 (arginine) to 0.98 (phenylalanine). In conclusion, enzyme supplementation did not significantly influence the digestibility coefficients of AA in rice bran. However, the inclusion of 10% and 20% RB supplemented with Ronozyme® can help to improve the methionine content in rice bran.

scholarly journals In vivo Determination of Amino Acid Bioavailability in Humans and Model Animals

2005 ◽  
Vol 88 (3) ◽  
pp. 923-934 ◽  
Author(s):  
Malcolm F Fuller ◽  
Daniel Tomé
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
The Body ◽  
Whole Body ◽  
Intestinal Lumen ◽  
Stable Isotope Labelling ◽  
Ileal Digestibility ◽  
Hydrolyzed Protein ◽  
Protein Free Diet

Abstract Because the digestion of many dietary proteins is incomplete, and because there is a continuous (but variable) entry into the intestinal lumen of endogenous protein and amino acid nitrogen that is also subject to digestion, the fluxes of nitrogen, amino acids, and protein in the gut exhibit a rather complicated pattern. Methods to distinguish and quantitate the endogenous and dietary components of nitrogen and amino acids in ileal chyme or feces include the use of a protein-free diet, the enzyme-hydrolyzed protein method, different levels of protein intake, multiple regression methods, and stable-isotope labelling of endogenous or exogenous amino acids. Assessment of bioavailability can be made, with varying degrees of difficulty, in man directly but, for routine evaluation of foods, the use of model animals is attractive for several reasons, the main ones being cost and time. Various animals and birds have been proposed as models for man but, in determining their suitability as a model, their physiological, enzymological, and microbiological differences must be considered. Fecal or ileal digestibility measurements, as well as apparent and true nitrogen and amino acid digestibility measurements, have very different nutritional significance and can, thus, be used for different objectives. Measurements at the ileal level are critical for determining amino acid losses of both dietary and endogenous origin, whereas measurements at the fecal level are critical in assessing whole-body nitrogen losses. A complementary and still unresolved aspect is to take into account the recycling of intestinal nitrogen and bacterial amino acids to the body.

scholarly journals Excessive heating of 00-rapeseed meal reduces not only amino acid digestibility but also metabolizable energy when fed to growing pigs

2020 ◽  
Vol 98 (7) ◽  
Author(s):  
Maryane S F Oliveira ◽  
Markus K Wiltafsky-Martin ◽  
Hans H Stein
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Basal Diet ◽  
Rapeseed Meal ◽  
Sole Source ◽  
Heating Time ◽  
Growing Pigs ◽  
Metabolizable Energy ◽  
Negative Effects ◽  
Ileal Digestibility

Abstract Two experiments were conducted to test the hypothesis that both the degree of heating and the time that heat is applied will affect the concentration of DE and ME, and the apparent ileal digestibility (AID) and the standardized ileal digestibility (SID) of amino acids (AA) in 00-rapeseed meal (00-RSM) fed to growing pigs. The nine treatments were prepared using a conventional 00-RSM that was either not autoclaved or autoclaved at 110 °C for 15 or 30 min or at 150 °C for 3, 6, 9, 12, 15, or 18 min. In experiment 1, 20 growing barrows with an average initial BW of 21.2 ± 1.2 kg were randomly allotted to the 10 diets in a replicated 10 × 4 Youden square with 10 diets and four periods in each square. A corn-based basal diet and nine diets containing corn and each source of 00-RSM were formulated. Urine and fecal samples were collected for 5 d after 7 d of adaptation. In experiment 2, nine diets contained one of the nine sources of 00-RSM as the sole source of AA, and an N-free diet that was used to measure basal endogenous losses of AA and CP was formulated. Twenty growing barrows with an initial BW of 69.8 ± 5.7 kg had a T-cannula installed in the distal ileum and were allotted to a 10 × 7 Youden square design with 10 diets and 7 periods. Ileal digesta were collected on days 6 and 7 of each 7-d period. Results from the experiments indicated that there were no effects of autoclaving at 110 °C on DE and ME or on AID and SID of AA in 00-RSM, but DE and ME, and AID and SID of AA were less (P &lt; 0.01) if 00-RSM was autoclaved at 150 °C compared with 110 °C. At 150 °C, there were decreases (quadratic, P &lt; 0.05) in DE and ME, and in AID and SID of AA as heating time increased. In conclusion, autoclaving at 110 °C did not affect ME or SID of AA in 00-RSM, but autoclaving at 150 °C had negative effects on ME and SID of AA and the negative effects increased as heating time increased.

scholarly journals Rumen degradability and ileal digestibility of proteins and amino acids of feedstuffs for cows

2014 ◽  
Vol 83 (3) ◽  
pp. 225-231
Author(s):  
Iveta Maskaľová ◽  
Vladimír Vajda ◽  
Marek Krempaský ◽  
Lukáš Bujňák
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Crude Protein ◽  
Amino Acid Profile ◽  
Ileal Digestibility ◽  
Corn Gluten ◽  
Corn Gluten Feed ◽  
Gluten Feed

Knowledge of the profile of amino acids of the rumen-undegradable protein can help in the formulation of diets to provide amino acids that complement microbial protein as well as supply amino acids, which are most limiting for milk production. Three non-lactating cows fitted with rumen cannulas were used to determine the effect ofin siturumen degradation on crude protein and amino acid profile of rumen-undegraded protein of feedstuffs. The obtained values of rumen degradability of crude protein with significant difference (P< 0.001) between feeds ranged from 20.3 to 76.3% (mean 62.0 ± 17.9%) and values of total amino acids ranged from 30.9% in corn gluten meal to 83.8% in corn gluten feed (mean 67.5 ± 16.4%). Anin vitromodified 3-step method was used to determine intestinal digestibility. Intestinal digestibility of undegraded protein varied from 54.5 ± 1.4% in raw soybean to 95.2 ± 1.0% in corn gluten feed. The absorbable amino acid profile of rumen-undegraded protein for each feedstuff was compared with profiles of the original feedstuff and the rumen-exposed undegraded protein. Absorbable lysine (9.3 ± 1.1 g/kg of crude protein) was higher in products of soybean and sunflower cake. Corn gluten feed and meal supplied more absorbable methionine (3.6 ± 0.6 g/kg of crude protein). This study showed that the digestibility factor of crude protein and amino acid based onin situandin vitromethods for thermal treatment of protein feeds can be used in models to optimize the amino acid nutrition of dairy cows and expand knowledge about rumen degradability and ileal digestibility of amino acids in feedstuffs.

scholarly journals The effect of dietary crude protein content on apparent and true ileal nitrogen and amino acid digestibilities

1994 ◽  
Vol 72 (1) ◽  
pp. 59-68 ◽  
Author(s):  
A. Donkoh ◽  
P. J. Moughan
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Content ◽  
Dietary Protein ◽  
Crude Protein ◽  
Apparent Digestibility ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Ileal Digestibility ◽  
Dietary Protein Level

The effect of dietary protein content (25, 60, 95, 130, 165 and 200 g crude protein (N × 6.25)/kg diet) on the apparent and true ileal digestibilities of N and amino acids in meat-and-bone meal given to the growing rat was investigated. Semi-synthetic diets in which meat-and-bone meal was the sole protein source were given to 180 g body-weight rats for 14 d. On the fourteenth day the rats were fed and then killed 4 h after the start of feeding and digesta were sampled from the terminal 200 mm ileum. Endogenous amino acid excretion was determined for eighteen rats given an enzymically hydrolysed casein (EHC)-based diet and with subsequent treatment of the digesta using ultrafiltration. The EHC-fed rats were killed 3 h after the start of feeding and digesta were collected from the terminal 200 mm ileum. True ileal digestibility values determined with reference to Cr as a marker were higher than the corresponding apparent estimates. Apparent digestibility values of N and amino acids increased significantly (P < 0.001) with increasing dietary protein level; however, dietary protein content had no significant (P > 0.05) effect on the true ileal digestibilities of N and amino acids. The mean apparent ileal digestibility of N in meat-and-bone meal ranged from 65.6 to 75.3%. The corresponding range for the true ileal digestibility of N was 76.9 to 78.2%. True ileal digestibility, unlike apparent digestibility, appears to be independent of dietary protein level and may allow feed ingredients to be compared accurately even if they are ingested in different quantities.

scholarly journals New Light on Old Fingermarks: The Detection of Historic Latent Fingermarks on Old Paper Documents Using 1,2-Indanedione/zinc

2020 ◽  
Author(s):  
Jemmy T. Bouzin ◽  
Jason Merendino ◽  
Stephen M. Bleay ◽  
Georgina Sauzier ◽  
Simon W. Lewis
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Zinc Chloride ◽  
Preliminary Investigation ◽  
High Quality ◽  
Different Ages ◽  
Over Time ◽  
Paper Substrates

This study explores trends in the effectiveness of 1,2-indandione/zinc chloride (IND/Zn) for visualizing latent fingermarks on paper substrates of various ages. Preliminary investigation of contemporaneous documents showed that high quality fingermarks could be deposited through incidental handling, although smudging and overlapping were evident. IND/Zn was then applied to incidentally handled documents up to 80 years old and successfully developed potentially identifiable fingermarks, significantly increasing the established timescale for fingermark detection with amino acid sensitive reagents. The results indicate that IND/Zn remains effective over longer periods than has been previously demonstrated, although a comparison between documents of different ages suggest that progressive diffusion of the target amino acids occurs over time, affecting the proportion of potentially identifiable marks.The findings of this study reinforce the applicability of IND/Zn for the detection of historic latent fingermarks on old paper documents.

scholarly journals The direct and difference procedures result in similar estimates for amino acid digestibility in feed ingredients fed to growing pigs

2020 ◽  
Vol 98 (8) ◽  
Author(s):  
Maryane S F Oliveira ◽  
John K Htoo ◽  
Hans H Stein
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Soybean Meal ◽  
Crude Protein ◽  
Sole Source ◽  
Growing Pigs ◽  
Cereal Grains ◽  
Ileal Digestibility ◽  
Wheat Middlings ◽  
The Difference

Abstract An experiment was conducted to test the hypothesis that values for standardized ileal digestibility (SID) of amino acids (AA) in corn, wheat, and wheat middlings obtained using the direct procedure are not different from values obtained using the difference procedure. Sixteen ileal-cannulated barrows (initial BW: 69.5 ± 5.0 kg) were allotted to a replicated 8 × 4 Youden Square design with 8 diets and 4 periods. Each period consisted of 5 d of adaptation to the diet and 2 d of collection of ileal digesta. Four diets were based on soybean meal (SBM), corn, wheat, or wheat middlings as the only AA-containing ingredients. Three additional diets were based on a mixture of SBM and corn, SBM and wheat, or SBM and wheat middlings, and an N-free diet was also used. The apparent ileal digestibility (AID) and the SID of crude protein (CP) and AA for the 4 diets containing SBM, corn, wheat, or wheat middlings as the sole source of AA were calculated using the direct procedure. The AID and SID of CP and AA for the 3 mixed diets containing SBM and corn, wheat, or wheat middlings were also calculated and the contribution of digestible AA from SBM was subtracted from the AID or SID values for the diets. The AID or SID of AA in corn, wheat, and wheat middlings were subsequently calculated by difference. Results indicated that the AID values for a few AA were lower (P &lt; 0.05) if the direct procedure was used instead of the difference procedure, regardless of ingredient. The AID of Trp was greater in corn and wheat middlings, and the SID of Trp in corn and wheat middlings tended to be greater, if the direct procedure rather than the difference procedure was used, but that was not the case for wheat (interaction, P &lt; 0.05 and P &lt; 0.10, respectively). However, for all other indispensable AA, and for most of the dispensable AA, the SID of AA in corn, wheat, and wheat middlings was not different between the difference procedure and the direct procedure. Therefore, values for SID of AA in cereal grains and fiber-rich ingredients may be determined using either the direct or the difference procedure.

Influence of xylanase supplementation on the apparent metabolisable energy and ileal amino acid digestibility in a diet containing wheat and oats, and on the performance of three strains of broiler chickens

1999 ◽  
Vol 50 (7) ◽  
pp. 1159 ◽  
Author(s):  
V. Ravindran ◽  
L. I. Hew ◽  
G. Ravindran ◽  
R. J. Gill ◽  
P. H. Pittolo ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Broiler Chickens ◽  
Dry Matter ◽  
Performance Parameters ◽  
Ileal Digestibility ◽  
Commercial Broiler ◽  
Metabolisable Energy ◽  
Digesta Viscosity

A commercial xylanase product was assessed for its effects on the performance of, and apparent metabolisable energy (AME) and ileal amino acid digestibility in, 3 commercial broiler strains (Strain A, B, and C) fed on a diet containing wheat (407 g/kg) and oats (78 g/kg). Exogenous xylanase improved weight gains (P = 0.07) and feed/gain (P < 0.04) of broilers, irrespective of genotype. Performance parameters significantly differed among the broiler strains. Strains A and C consumed more (P < 0.04) feed, and grew faster (P < 0.01) and more efficiently (P = 0.07) than Strain B. Enzyme supplementation resulted in 2.8% improvement (P < 0.05) in the AME of the wheat-based diet. The AME tended to be higher (P = 0.09) with Strain A (13.46 MJ/kg dry matter) and Strain C (13.57 MJ/kg dry matter) than with Strain B (13.10 MJ/kg dry matter). The apparent ileal digestibility of all amino acids was 1–2 percentage units higher in birds fed on the enzyme-supplemented diet than in those fed on the unsupplemented diet (controls), but the differences were significant (P < 0.05) only for threonine, methionine, isoleucine, arginine, aspartic acid, serine, and glutamic acid. Highly significant (P < 0.001) strain effects were observed for apparent ileal digestibility of all amino acids, with Strain A recording the highest (0.807–0.945) and Strain B the lowest (0.710–0.912). Mean ileal digestibility coefficients of the 15 amino acids in wheat-based diets for Strains A, B, and C were 0.858, 0.791, and 0.828, respectively. Ileal digesta viscosity was low, and was not affected by dietary enzyme, suggesting that other factors were responsible for the observed improvements in bird performance.

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