Impact of Heat Stress on Meat Quality and Antioxidant Markers in Iberian Pigs

Heat stress is associated with impaired meat quality and disruption of redox balance. This study investigated the effect of chronic exposure to high temperature on meat quality and antioxidant markers of muscles (longissimus lumborum and gluteus medius) of growing Iberian pigs. Twenty-four pure Iberian pigs were allocated during 28 days to one of three treatments (n = 8/treatment): thermoneutral conditions (20 °C) and ad libitum feeding (TN), heat stress conditions (30 °C) and ad libitum feeding (HS) and thermoneutral and pair-fed with HS (TN-pf). Muscles of the HS group had greater intramuscular fat content than the TN-pf group and higher Zn levels than TN and TN-pf, whereas differences on fatty acid composition were negligible. Heat exposure did not affect pH, color coordinates of redness (a*) and yellowness (b*) and MDA values but had a positive influence on lightness and drip losses. Moreover, chronic heat stress stimulated the activity of antioxidant defenses SOD, CAT and GPx. The statistical factor analysis adequately classified the muscles studied, but was unable to differentiate samples according with treatments. Findings of the present study support an adaptive response of the Iberian pig to high temperatures and show the high Iberian meat quality even under adverse climate situations.

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Identification of Metabonomics Changes in Longissimus Dorsi Muscle of Finishing Pigs Following Heat Stress through LC-MS/MS-Based Metabonomics Method

Animals ◽

10.3390/ani10010129 ◽

2020 ◽

Vol 10 (1) ◽

pp. 129 ◽

Cited By ~ 2

Author(s):

Jie Gao ◽

Peige Yang ◽

Yanjun Cui ◽

Qingshi Meng ◽

Yuejin Feng ◽

...

Keyword(s):

Heat Stress ◽

Ambient Temperature ◽

Meat Quality ◽

Ultra Performance Liquid Chromatography ◽

Longissimus Dorsi ◽

Finishing Pigs ◽

Longissimus Dorsi Muscle ◽

Inosinic Acid ◽

Ad Libitum ◽

Ad Libitum Feeding

Heat stress (HS) negatively affects meat quality by affecting material and energy metabolism, and exploring the mechanism underlying the muscle response to chronic HS in finishing pigs is important for the global pork industry. This study investigated changes in the metabolic profiles of the longissimus dorsi (LD) muscle of finishing pigs under high temperature using ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOF-MS) and multivariate data analysis (MDA). Castrated male DLY pigs (Duroc × Landrance × Yorkshire pigs, n = 24) from 8 litters were divided into three treatment groups: constant optimal ambient temperature at 22 °C and ad libitum feeding (CR, n = 8); constant high ambient temperature at 30 °C and ad libitum feeding (HS, n = 8); and constant optimal ambient temperature 22 °C and pair-feeding to the control pigs (PF, n = 8). The metabolic profile data from LD muscle samples were analyzed by MDA and external search engines. Nine differential metabolites (L-carnosine, acetylcholine, inosinic acid, L-carnitine, L-anserine, L-α-glycerylphosphorylcholine, acetylcarnitine, thiamine triphosphate, and adenosine thiamine diphosphate) were involved in antioxidant function, lipid metabolism, and cell signal transduction, which may decrease post mortem meat quality and play important roles in anti-HS. Four metabolites (L-carnosine, acetylcholine, inosinic acid, and L-carnitine) were verified, and it was indicated that the muscle L-carnitine content was significantly lower in HS than in CR (p < 0.01). The results show that constant HS affects the metabolites in the LD muscle and leads to coordinated changes in the endogenous antioxidant defense and meat quality of finishing pigs. These metabonomics results provide a basis for researching nutritional strategies to reduce the negative effects of heat stress on livestock and present new insights for further research.

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Effects of heat stress on growth performance, selected physiological and immunological parameters, caecal microflora, and meat quality in two broiler strains

Asian-Australasian Journal of Animal Sciences ◽

10.5713/ajas.19.0208 ◽

2020 ◽

Vol 33 (5) ◽

pp. 778-787 ◽

Cited By ~ 5

Author(s):

Elmutaz Atta Awad ◽

Muhamad Najaa ◽

Zainool Abidin Zulaikha ◽

Idrus Zulkifli ◽

Abdoreza Farjam Soleimani

Keyword(s):

Heat Stress ◽

Growth Performance ◽

Meat Quality ◽

Acute Phase Proteins ◽

Body Weight Gain ◽

Heat Exposure ◽

Broiler Chicks ◽

Feed Conversion ◽

Immunological Parameters ◽

Temperature Interactions

Objective: This study was conducted to investigate the effects of normal and heat stress environments on growth performance and, selected physiological and immunological parameters, caecal microflora and meat quality in Cobb 500 and Ross 308 broilers.Methods: One-hundred-and-twenty male broiler chicks from each strain (one-day-old) were randomly assigned in groups of 10 to 24 battery cages. Ambient temperature on day (d) 1 was set at 32°C and gradually reduced to 23°C on d 21. From d 22 to 35, equal numbers of birds from each strain were exposed to a temperature of either 23°C throughout (normal) or 34°C for 6 h (heat stress).Results: From d 1 to 21, strain had no effect (p>0.05) on feed intake (FI), body weight gain (BWG), or the feed conversion ratio (FCR). Except for creatine kinase, no strain×temperature interactions were observed for all the parameters measured. Regardless of strain, heat exposure significantly (p<0.05) reduced FI and BWG (d 22 to 35 and 1 to 35), immunoglobulin Y (IgY) and IgM, while increased FCR (d 22 to 35 and 1 to 35) and serum levels of glucose and acute phase proteins (APPs). Regardless of temperature, the Ross 308 birds had significantly (p<0.05) lower IgA and higher finisher and overall BWG compared to Cobb 500.Conclusion: The present study suggests that the detrimental effects of heat stress are consistent across commercial broiler strains because there were no significant strain×temperature interactions for growth performance, serum APPs and immunoglobulin responses, meat quality, and ceacal microflora population.

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A comparative study of Hereford and Friesian steers. 2. Carcass characteristics and meat quality

Australian Journal of Agricultural Research ◽

10.1071/ar9820157 ◽

1982 ◽

Vol 33 (1) ◽

pp. 157 ◽

Cited By ~ 2

Author(s):

DG Taylor

Keyword(s):

Comparative Study ◽

Meat Quality ◽

Carcass Characteristics ◽

Fat Content ◽

The Other ◽

Bone Content ◽

Retail Meat ◽

Ad Libitum ◽

Dressing Percentage ◽

Ad Libitum Feeding

Two trials, each involving four Hereford and four Friesian steers, were finalized with the slaughtering of the animals and comparison of their carcass characteristics and meat quality. One trial involved hand-feeding the animals to achieve a constant liveweight gain of 0.68 kg/day from 6 months of age to slaughter at 15 months of age; the other trial used animals of the same age, but on ad libitum feeding. Carcass data substantiated the subjective observation that the Friesian carcasses were longer, less compact and displayed less desirable 'beef type' conformation than those of the Hereford. The dressing percentage of the Friesians was significantly (P < 0.01) lower than that of the Herefords. The Hereford carcasses had a significantly greater fat content than the Friesians, but lower percentages of both muscle and bone. The Hereford produced the greater yield of boneless retail meat (not fat trimmed), which could be attributed to their greater carcass fat and lower carcass bone content. The meat from both breeds was of equivalent palatability when assessed subjectively for tenderness, juiciness and flavour, and objectively for tenderness.

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Dietary Betaine Reduces the Negative Effects of Cyclic Heat Exposure on Growth Performance, Blood Gas Status and Meat Quality in Broiler Chickens

Agriculture ◽

10.3390/agriculture10050176 ◽

2020 ◽

Vol 10 (5) ◽

pp. 176

Author(s):

Majid Shakeri ◽

Jeremy J. Cottrell ◽

Stuart Wilkinson ◽

Hieu H. Le ◽

Hafiz A. R. Suleria ◽

...

Keyword(s):

Heat Stress ◽

Growth Performance ◽

Meat Quality ◽

Broiler Chickens ◽

Control Diet ◽

Heat Exposure ◽

Thiobarbituric Acid ◽

Respiratory Alkalosis ◽

Meat Tenderness ◽

Growing Period

Heat stress (HS) impairs growth performance and has a severe impact on lipid and protein metabolism, leading to serious adverse effects on meat quality. Forty-eight day-old-male Ross-308 chicks were assigned to two temperature conditions, thermoneutral or cyclical HS, and fed with either a control diet (CON) or the CON plus betaine (BET). Heat stress increased rectal temperature (p < 0.001), respiration rate (p < 0.001) and increased blood pH (p = 0.017), indicating that HS caused respiratory alkalosis. Heat stress reduced body weight during the final stage of growing period (p = 0.005), while BET improved it (p = 0.023). Heat stress tended to reduce breast muscle water content and drip loss (p = 0.089 and p = 0.082), while both were improved with BET (p = 0.008 and p = 0.001). Heat stress tended to reduce the myofibril fragmentation index (p = 0.081) whereas it increased with BET (p = 0.017). Heat stress increased thiobarbituric acid reactive substances (p = 0.017), while BET improved it (p = 0.008). Meat tenderness was not affected by HS, but was improved with BET (p < 0.001). In conclusion, BET improved growth performance over the latter stages of the growing period, and improved product quality of broiler chickens when chickens exposed to HS.

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Effect of heat stress on blood parameter, carcass and meat quality of Black Bengal goat

Bangladesh Journal of Animal Science ◽

10.3329/bjas.v42i1.15783 ◽

2013 ◽

Vol 42 (1) ◽

pp. 57-61 ◽

Cited By ~ 9

Author(s):

MA Hashem ◽

MM Hossain ◽

MS Rana ◽

MM Hossain ◽

MS Islam ◽

...

Keyword(s):

Heat Stress ◽

Meat Quality ◽

Heat Exposure ◽

Cooking Loss ◽

Blood Parameter ◽

Goat Meat ◽

Heat Treated ◽

Humidity Index ◽

Black Bengal Goat

The research was conducted to study the effect of heat stress on blood parameter, carcass characteristics and meat quality of the Black Bengal goat. Nine goats were randomly and equally divided into three groups having almost same age and weight. Three groups were divided as zero hour (T0), four hours (T4) and eight hours (T8) of heat exposure. The T0 group were not exposed to heat stress, the T4 and T8 group were exposed to heat by keeping them at outside for 4 (four) hours and 8 (eight) hours from 9.00 AM to 1.00 PM and from 9.00 AM to 5.00 PM, respectively. Temperaturehumidity index (THI) was calculated as 26.88 indicating all the experimental animals were in extreme severe heat stress. The blood parameter such as RBC, PCV%, Hb%, WBC was significantly (p<0.01) higher in T8 group than those of T4 and T0 groups. There was also significant (p<0.05) effect of heat stress on pH of the meat from different groups of the Black Bengal goats. By-products e.g., blood, pluck, spleen and kidney, and cooking loss were significantly (p<0.01) increased with the increase of the heat, but no significant differences were observed for pre-slaughter parameters, carcass weight, drip loss, dressing percentage, and proximate composition of the goat meat for DM, CP and Ash, while the EE significantly (p<0.05) differed among the heat treated groups. Heat stress significantly affected the blood parameter, cooking loss, pH, by-product of goats rather than non heat stressed group. It indicates that heat stress limits the qualitative and quantitative production characteristics of goat meat. DOI: http://dx.doi.org/10.3329/bjas.v42i1.15783 Bang. J. Anim. Sci. 2013. 42 (1): 57-61

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Chromium supplementation modulates glucose metabolism in heat-stressed Girolando dairy cows

Semina Ciências Agrárias ◽

10.5433/1679-0359.2020v41n5supl1p2445 ◽

2020 ◽

Vol 41 (5supl1) ◽

pp. 2445-2452

Author(s):

Lilian dos Santos Ribeiro ◽

Felipe Zandonadi Brandão ◽

Luciano de Rezende Carvalheira ◽

Diana Rangel de Lemos ◽

Gustavo Torres de Souza ◽

...

Keyword(s):

Heat Stress ◽

Glucose Metabolism ◽

Phosphoenolpyruvate Carboxykinase ◽

Growth Hormone Receptor ◽

Control Diet ◽

Stress Conditions ◽

Climatic Chamber ◽

Glucose Transporter 2 ◽

Ad Libitum ◽

Ad Libitum Feeding

The objective of this study was to evaluate the effects of dietary chromium supplementation on hepatic gene expression of lactating Girolando (Holstein x Gyr) cows under heat stress conditions in climatic chamber. Thirty-six ¾ Holstein x Gyr lactating cows were used, based on a 2x3 factorial scheme, to evaluate the effects of two diets (0 vs 0.50 mg of organic chromium kg-1 dry matter) and three environmental conditions (ECs): heat stress conditions in climatic chamber with ad libitum feeding (HS), a thermoneutral environment with ad libitum feeding (TN), and a pair-fed group in a thermoneutral environment (PF). Under HS group, the expression levels of glucose transporter 2 (GLUT2), glucose-6-phosphatase (G6Pase), and growth hormone receptor (rGH) were downregulated (P < 0.05) in chromium-supplemented cows compared to those in cows fed the control diet. GLUT2 expression was upregulated (P = 0.02) in the HS group and insulin-like growth factor 1 (IGF1) was downregulated (P < 0.01) in the PF group in cows fed the control diet compared to the expression in the TN group. No differences were observed between the ECs in terms of relative abundances of GLUT2, phosphoenolpyruvate carboxykinase (PEPCK), G6Pase, rGH, and IGF1 transcripts among the chromium-supplemented cows (P > 0.05). Heat stress caused changes in the expression of genes related to glucose metabolism, and organic chromium could modulate glucose metabolism in animals under heat stress conditions to some extent.

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A Dietary Sugarcane-Derived Polyphenol Mix Reduces the Negative Effects of Cyclic Heat Exposure on Growth Performance, Blood Gas Status, and Meat Quality in Broiler Chickens

Animals ◽

10.3390/ani10071158 ◽

2020 ◽

Vol 10 (7) ◽

pp. 1158

Author(s):

Majid Shakeri ◽

Jeremy J. Cottrell ◽

Stuart Wilkinson ◽

Hieu H. Le ◽

Hafiz A. R. Suleria ◽

...

Keyword(s):

Heat Stress ◽

Growth Performance ◽

Meat Quality ◽

Broiler Chickens ◽

Control Diet ◽

Heat Exposure ◽

Dependent Manner ◽

Final Body Weight ◽

Dietary Polyphenols ◽

Temperature Conditions

Heat stress (HS) compromises growth performance and meat quality of broiler chickens by interrupting lipid and protein metabolism, resulting in increased oxidative damages. The experiment attempted to investigate whether dietary polyphenols (Polygain (POL)) could ameliorate the aforementioned adverse effects of HS on performance and meat quality. One hundred and twenty one day-old-male chicks were allocated to two temperature conditions, thermoneutral (TN) or HS, and fed with either a control diet (CON) or the CON plus four different doses of POL (2, 4, 6 and 10 g/kg). Heat stress caused respiratory alkalosis as evidenced by increased rectal temperature (p < 0.001) and respiration rate (p < 0.001) due to increased blood pH (p < 0.001). Heat stress decreased final body weight (p = 0.061) and breast muscle water content (p = 0.013) while POL improved both (p = 0.002 and p = 0.003, respectively). Heat stress amplified muscle damages, indicated by increased thiobarbituric acid reactive substances (p < 0.001) and reduced myofibril fragmentation index (p = 0.006) whereas POL improved both (p = 0.037 and p = 0.092, respectively). Heat stress impaired meat tenderness (p < 0.001) while POL improved it (p = 0.003). In conclusion, HS impaired growth performance and meat quality whereas POL ameliorated these responses in a dose-dependent manner, and effects of POL were evident under both temperature conditions.

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Benefits of Magnesium Supplementation to Broiler Subjected to Dietary and Heat Stress: Improved Redox Status, Breast Quality and Decreased Myopathy Incidence

Antioxidants ◽

10.3390/antiox8100456 ◽

2019 ◽

Vol 8 (10) ◽

pp. 456 ◽

Cited By ~ 4

Author(s):

Estevez ◽

Petracci

Keyword(s):

Heat Stress ◽

Meat Quality ◽

Control Diet ◽

Redox Status ◽

Negative Influence ◽

Positive Influence ◽

Positive Effects ◽

Production Parameters ◽

Oxidized Oil ◽

Oxidative Reactions

Poultry is highly sensitive to oxidative reactions. Oxidative reactions have attracted considerable attention from animal and food scientists because of the adverse effects of these reactions on animal welfare, performance and food quality. Despite its implication in multiple biological functions magnesium (Mg) supplementation is typically overlooked in broiler diets. The objective of this study was to evaluate the effect of Mg supplementation (0.3%) using a commercial product (Optibreast®) on production parameters, the redox status and meat quality in broilers challenged with dietary (oxidized oil) and heat stress. The incidence of myopathies, namely, wooden breast (WB) and white striping (WS) was also assessed. Mg supplementation had a clear interaction with the absorption/accumulation of Ca in blood and breast muscle but this effect had no negative influence on any of the production parameters under study. Mg supplementation had positive effects on particular meat quality traits such as water holding capacity (WHC) and color. WHC may have other positive effects in turn on relevant sensory traits such as juiciness. Mg supplementation protected against protein oxidation in liver and plasma of broilers. This effect may be related to the increased activity of catalase in such tissues. Mg supplementation reduced the incidence of WS and WB myopathies to almost half the occurrence of such defects in animals fed a control diet. Further studies with a larger number of animals and the application of advanced proteomic/metabolomic tools are required to (1) corroborate the positive influence of Mg on myopathy incidence and (2) identify the underlying molecular basis of the proposed mechanisms.

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The Effect of Feeding System on the Carcass Quality of Crossbred Lambs with Texel

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis ◽

10.11118/actaun202068010017 ◽

2020 ◽

Vol 68 (1) ◽

pp. 17-24

Author(s):

Angela Cividini ◽

Dušan Terčič ◽

Mojca Simčič

Keyword(s):

Growth Rate ◽

Meat Quality ◽

Carcass Quality ◽

Quality Traits ◽

Feeding System ◽

Ad Libitum ◽

Daily Gain ◽

Effect Of Feeding ◽

Carcass Cuts

The aim of this study was to estimate the effect of feeding system on the growth rate and carcass quality of crossbred Improved Jezersko-Solčava x Texel (JSRT) lambs and to evaluate the effect of sex on these traits. The trial was conducted in nature according to the traditional rearing systems. The trial included 44 crossbred lambs, which were born and reared until the slaughter in three different flocks. In the age of 10 days suckled lambs were offered with ad libitum corresponding diets according to the feeding system. All lambs were slaughtered in seven consecutive days by the same procedure. The effect of feeding system significantly affected daily gain from birth to slaughter, EUROP carcass conformation and shoulder width. Likewise, the effect of sex significantly affected daily gain from birth to slaughter and internal fatness of carcasses. According to carcass cuts the feeding system significantly affected only the proportion of neck and leg. Considering meat quality traits, feeding system had a significant effect on the pH 45 and CIE a* values. In this study, we could speculate that more than the feeding system the growth and the carcass traits as well as meat traits were affected by the amount of the supplement.

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Mitochondrial Sirtuins in Reproduction

Antioxidants ◽

10.3390/antiox10071047 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1047

Author(s):

Giovanna Di Emidio ◽

Stefano Falone ◽

Paolo Giovanni Artini ◽

Fernanda Amicarelli ◽

Anna Maria D’Alessandro ◽

...

Keyword(s):

Stress Responses ◽

Metabolic Pathways ◽

Redox Balance ◽

Antioxidant Defenses ◽

Metabolic Abnormalities ◽

Female Reproduction ◽

Mitochondrial Dysfunctions ◽

Early Embryos ◽

The Relationship

Mitochondria act as hubs of numerous metabolic pathways. Mitochondrial dysfunctions contribute to altering the redox balance and predispose to aging and metabolic alterations. The sirtuin family is composed of seven members and three of them, SIRT3-5, are housed in mitochondria. They catalyze NAD+-dependent deacylation and the ADP-ribosylation of mitochondrial proteins, thereby modulating gene expression and activities of enzymes involved in oxidative metabolism and stress responses. In this context, mitochondrial sirtuins (mtSIRTs) act in synergistic or antagonistic manners to protect from aging and aging-related metabolic abnormalities. In this review, we focus on the role of mtSIRTs in the biological competence of reproductive cells, organs, and embryos. Most studies are focused on SIRT3 in female reproduction, providing evidence that SIRT3 improves the competence of oocytes in humans and animal models. Moreover, SIRT3 protects oocytes, early embryos, and ovaries against stress conditions. The relationship between derangement of SIRT3 signaling and the imbalance of ROS and antioxidant defenses in testes has also been demonstrated. Very little is known about SIRT4 and SIRT5 functions in the reproductive system. The final goal of this work is to understand whether sirtuin-based signaling may be taken into account as potential targets for therapeutic applications in female and male infertility.

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