Elevated CO2 Enhanced the Antioxidant Activity and Downregulated Cell Wall Metabolism of Wolfberry (Lycium barbarum L.)

Modified atmosphere packaging (MAP) has been widely known to delay the postharvest fruit senescence; nevertheless, its effect on antioxidant activity and cell wall metabolism of wolfberry fruit is largely unknown. The present study investigated the impact of elevated CO2 on the quality attributes and cell wall degradation of wolfberry fruit during storage. The results showed that 10% CO2 better maintained the physiological quality and conferred the reduction in weight loss, decay index, and color change. Higher 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-1-picrylhydrazil (DPPH) radical scavenging activity, total phenol and flavonoid content, and superoxide dismutase (SOD) and catalase (CAT) activity of wolfberry were detected at elevated CO2 concentrations. Elevated CO2 atmosphere contributed to the maintenance of the cell integrity, the decrease of cell wall degradation (polygalacturonase, pectate lyase, cellulase, and β-glucosidase), and the increase of cellulose and proto pectin content. Overall, we revealed the potential mechanism of elevated CO2 on the antioxidant activity enhancement and cell wall homeostasis of fresh berry fruit.

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The impact of UV-B radiation on antioxidant activity, essential oil composition and physiological factors of Pelargonium graveolens L’Hér.

Acta agriculturae Slovenica ◽

10.14720/aas.2019.113.2.2 ◽

2019 ◽

Vol 113 (2) ◽

pp. 211 ◽

Cited By ~ 1

Author(s):

Marjan AZARAFSHAN ◽

Maryam PEYVANDI ◽

Hossein ABBASPOUR ◽

Zahra NOORMOHAMMADI ◽

Ahmad MAJD

Keyword(s):

Antioxidant Activity ◽

Antioxidant Enzymes ◽

Essential Oils ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Dry Mass ◽

Carbohydrate Content ◽

Oil Composition ◽

Pelargonium Graveolens ◽

The Impact

<em>Pelargonium graveolens</em> L’Hér. is an important aromatic and medicinal plant, which is famous for its essential oils (EOS).The aim of this study was to evaluate the effects of UV-B on growth factors, essential oils components, antioxidant activity of essential oils and antioxidant enzymes activity, carbohydrate content, leaf pigments and total protein. Cuttings from potted plants were exposed to 0, 0.12, 0.26, and 0.38 W m-2 of UV-B radiation. The antioxidant enzyme activity, carbohydrate content and protein and pigments contents were measured by spectrophotometric methods. The composition of EOS was analyzed by GC-MS. The antioxidant activity of the EOS was analyzed by free radical scavenging activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH). Results demonstrated that the leaves’ fresh and dry mass, plant height, number of leaves, and the content of chlorophyll, protein and total carbohydrates were significantly (P ≤ 0.05) decreased, when plants were subjected to increased intensity of UV radiation. In contrast, the content of carotenoids and antioxidant enzymes activities increased. The chemical composition of EOS indicated that the main components in all treated plants were citronellol, geraniol, and citronellyl formate. The antioxidant activity of the essential oils increased with increasing UV-B radiation. The maximum and minimum IC50 values of essential oils were obtained in control plants and plants under 0.38 W m-2 UV-B radiation, respectively.

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The Utilization of Vegetable and Fruit Wastes for Saccharomyces cerevisieae Cell Wall Based β-Glucan Production with Antioxidant Activity

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis ◽

10.11118/actaun202068010119 ◽

2020 ◽

Vol 68 (1) ◽

pp. 119-127

Author(s):

Gemilang Lara Utama ◽

Florensia Irena ◽

Elazmanawati Lembong ◽

Indira Lanti Kayaputri ◽

Tensiska Tensiska ◽

...

Keyword(s):

Antioxidant Activity ◽

Cell Wall ◽

Radical Scavenging Activity ◽

Descriptive Analysis ◽

Radical Scavenging ◽

Fermentation Medium ◽

Scavenging Activity ◽

Fruit Wastes

The study aims to determine the number of mass and antioxidant activity of β-glucan extracted from S.cerevisieae which grown on vegetable and fruit wastes. The method used is experimental with descriptive analysis which consisted of 3 treatments namely banana waste, papaya waste, and napa cabbage waste as fermentation medium, repeated thrice. Fermentation medium was made by mixing waste with water with the ratio of 1:2. Ten percent (w/v) of S.cerevisieae was inoculated and incubated for 48 hours, at 27 ℃. Extraction of β-glucan was carried out using acid-alkaline methods and antioxidant activity was tested by DPPH (2,2-Diphenyl-1-picrylhydrayl) method and the microstructure of β-glucan is determined by Scanning Electrone Microscope. The result showed that the best medium in producing β-glucan was papaya waste which resulting 19.094 g β-glucan mass, with radical scavenging activity of 20.71 % and globular diameter of 533 μm.

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Evaluation of antioxidant and Anthelmintic activity of methanolic flower extract of Nyctanthes arbor-tristis

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v9i1.1177 ◽

2018 ◽

Vol 9 (1) ◽

pp. 78

Author(s):

Vijaya Jyothi M ◽

Bhargav E ◽

Pavan Kumar K ◽

Praneeth Gowd K ◽

Ram Pavan S

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging Activity ◽

Iridoid Glycosides ◽

Radical Scavenging ◽

Anthelmintic Activity ◽

Total Phenolic ◽

Standard Drug ◽

Flower Extract ◽

Reducing Ability ◽

Total Flavonoids Content

Nyctanthes arbour-tristis is a shrub belongs to the family Oleaceae. The flowers of this plant are fragrant since the presence of flavonol glycosides. It has also been reported for the presence of β-sitosterol, iridoid glycosides, tannins etc., and known to have immunostimulant, hepatoprotective, antiviral and antifungal activities. In the present study an attempt is made to identify antioxidant capacity and anthelminthic potential of methanolic flower extract of Nyctanthes arbour-tristis. Antioxidant activity was evaluated by total phenolic content assay, total flavonoids content assay, free radical scavenging activity and reducing ability methods. Anthelmintic activity was evaluated on Perithima posthuma using Piperazine citrate as standard drug. The results obtained for the above activities reveals that Nyctanthes arbour-tristis shows considerable antioxidant activity for all the methods and anthelminthic potential at 300 mg/ml. Keywords: arbour-tristis; antioxidant activity; anthelminthic activity; Perithima posthuma; Piperazine citrate.

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Assessment of antioxidant and antibacterial activities of crude extracts of verbena officinalis Linn root or Atuch (Amharic)

10.31221/osf.io/pzh3s ◽

2019 ◽

Cited By ~ 1

Author(s):

Chem Int

Keyword(s):

Antioxidant Activity ◽

Methanolic Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Dry Weight ◽

Antimicrobial Activities ◽

Total Flavonoid ◽

Root Extract ◽

Antioxidant Power ◽

Crude Extracts

Verbena officinalis Linn is a traditionally known medicinal plant which is used against a number of diseases including inflammatory conditions. In this study its antioxidant activity (reducing powers, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities), ferric reduction activity potential (FRAP), total flavonoid concentration and antimicrobial activities of 80%, 90%, 100% methanol and chloroform extracts of V. officinalis Linn root and 90% and100% methanol leaf extracts were determined. Its antioxidant activity increases with increase in amount of extract (10% to 40%v/v). Total flavonoid content (TFC) varied from 73.32±0.002 mgQE/100g of dry weight (90% methanol) to 42.39±0.032 mgQE/100g dry weight (chloroform), 2,2-diphenyl-1-picrylhydrazyl (DPPH), radical scavenging activity (%) was varied between 87.39% (90% methanol) to 45.57% (chloroform) while Ferric reducing antioxidant power was observed between 372.93±0.04 mgAAE/100 g extract (90% methanol) to 129.41±0.026 mgAAE/100 g chloroform in the root extract. The methanolic extract of the leaf showed less antioxidant activity than the methanolic extract of the root. Crude extracts of V. officinalis root showed various degree of antimicrobial activity towards drug resistance microbial pathogens. Growth inhibition tests against bacterial pathogens demonstrated concentration dependence. Moreover, gram positive bacteria were more susceptible to V. officinalis root extract when compared to gram negative bacteria. In general V. officinalis root and leave extracts possess strong antioxidant and antimicrobial activities.

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Antioxidant activity of cookiessupplemented with sugarbeet dietary fiber

Sugar Industry ◽

10.36961/si11177 ◽

2011 ◽

pp. 151-157 ◽

Cited By ~ 2

Author(s):

Marijana B. Saka ◽

Julianna F. Gyura ◽

Aleksandra Mišan ◽

Zita I. Šereš ◽

Biljana S. Pajin ◽

...

Keyword(s):

Antioxidant Activity ◽

Shelf Life ◽

Dietary Fiber ◽

Wheat Flour ◽

Room Temperature ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Functional Characteristics

The antioxidant activity of cookies prepared by the addition of sugarbeet dietary fibers was investigated in order to estimate their influence on functional characteristics and shelf-life of cookies. Treated fiber (TF) was obtained from sugarbeet by extraction with sulfurous acid (75 °C at pH = 5.7during 60 min) and treatment with hydrogen peroxide (20 g/LH2O2 at pH = 11 during 24 h). The fiber obtained was dried (80 °C), ground and sieved. TF was investigated in comparison with commercially available Fibrex®. The cookies were prepared by the addition of 0, 7, 9 and 11% of sugarbeet dietary fiber as a substitute for wheat flour in the formulation of cookies. The antioxidant properties of cookies were tested every 7 days using a DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity test during 6 weeks of storage at room temperature (23 ± 1 ºC). The obtained results indicated that substitution of wheat flour with Fibrex® in the formulation of cookies upgraded the antioxidant activity, i.e. the functional characteristics of Fibrex®-enriched cookies and could prolong their shelf-life. In contrast, TF did not increase the antioxidant activity of TF-enriched cookies. The better antioxidant activities of Fibrex®-enriched cookies could be attributed to the presence of ferulic acid.

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Curcumin-based antioxidant and glycohydrolase inhibitor compounds: Synthesis and in vitro appraisal of the dual activity against diabetes

Medicinal Chemistry ◽

10.2174/1573406416666200506083718 ◽

2020 ◽

Vol 16 ◽

Author(s):

Sajjad Esmaeili ◽

Nazanin Ghobadi ◽

Donya Nazari ◽

Alireza Pourhossein ◽

Hassan Rasouli ◽

...

Keyword(s):

Antioxidant Activity ◽

Enzyme Inhibitor ◽

Radical Scavenging Activity ◽

Curcuma Longa ◽

Radical Scavenging ◽

Reducing Power ◽

Inhibitory Effect ◽

Curcumin Derivatives ◽

Reducing Power Assay

Background: Curcumin, as the substantial constituent of the turmeric plant (Curcuma longa), plays a significant role in the prevention of various diseases, including diabetes. It possesses ideal structure features as enzyme inhibitor, including a flexible backbone, hydrophobic nature, and several available hydrogen bond (H-bond) donors and acceptors. Objective: The present study aimed at synthesizing several novel curcumin derivatives and further evaluation of these compounds for possible antioxidant and anti-diabetic properties along with inhibitory effect against two carbohydrate-hydrolyzing enzymes, α-amylase and α-glucosidase, as these enzymes are therapeutic targets for attenuation of postprandial hyperglycemia. Methods: Therefore, curcumin-based pyrido[2,3-d]pyrimidine derivatives were synthesized and identified using an instrumental technique like NMR spectroscopy and then screened for antioxidant and enzyme inhibitory potential. Total antioxidant activity, reducing power assay and 1,1-diphenyl-2-picrylhydrazyl (DPPH• ) radical scavenging activity were done to appraisal the antioxidant potential of these compounds in vitro. Results: Compounds L6-L9 showed higher antioxidant activity while L4, L9, L12 and especially L8 exhibited the best selectivity index (lowest α-amylase/α-glucosidase inhibition ratio). Conclusion: These antioxidant inhibitors may be potential anti-diabetic drugs, not only to reduce glycemic index but also to limit the activity of the major reactive oxygen species (ROS) producing pathways.

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Comparison of Phenolic Contents and Scavenging Activities of Miang Extracts Derived from Filamentous and Non-Filamentous Fungi-Based Fermentation Processes

Antioxidants ◽

10.3390/antiox10071144 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1144

Author(s):

Aliyu Dantani Abdullahi ◽

Pratthana Kodchasee ◽

Kridsada Unban ◽

Thanawat Pattananandecha ◽

Chalermpong Saenjum ◽

...

Keyword(s):

Filamentous Fungi ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Protective Effects ◽

Tea Leaves ◽

Antioxidant Power ◽

Phenolic Contents ◽

Ic50 Values ◽

Ferric Reducing Antioxidant Power ◽

The Impact

The study investigated the impact of the fermentation process on the phenolic contents and antioxidant and anti-inflammatory activities in extracts of Miang, an ethnic fermented tea product of northern Thailand. The acetone (80%) extraction of Miang samples fermented by a non-filamentous fungi-based process (NFP) and filamentous fungi-based process (FFP) had elevated levels of total polyphenols, total tannins, and condensed tannins compared to young and mature tea leaves. The antioxidant studies also showed better the half-maximal inhibitory concentration (IC50) values for fermented leaves in both 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity assays as well as improved ferric reducing antioxidant power (FRAP) compared to young and mature tea leaves. Extracts of NFP and FFP samples at concentrations of 50 and 100 ppm showed better protective effects against hydrogen peroxide (H2O2)-induced intracellular reactive oxygen species (ROS) production in HT-29 colorectal cells without exerting cytotoxicity. Additionally, lipopolysaccharide (LPS)-induced production of nitric oxide (a proinflammatory mediator as well as a reactive nitrogen species) was also inhibited by these fermented Miang extracts with an IC50 values of 17.15 μg/mL (NFP), 20.17 μg/mL (FFP), 33.96 μg/mL (young tea leaves), and 31.33 μg/mL (mature tea leaves). Therefore, both NFP-Miang and FFP-Miang showed the potential to be targeted as natural bioactive functional ingredients with preventive properties against free radical and inflammatory-mediated diseases.

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Antioxidant Activity in Rheum emodi Wall (Himalayan Rhubarb)

Molecules ◽

10.3390/molecules26092555 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2555

Author(s):

Sang Koo Park ◽

Yoon Kyung Lee

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Ethyl Acetate Fraction ◽

Total Phenolic ◽

Scavenging Activity ◽

Polyphenol Content ◽

Abts Assay ◽

Ic50 Values

Using natural products as antioxidant agents has been beneficial to replace synthetic products. Efforts have been made to profile the antioxidant capacities of natural resources, such as medicinal plants. The polyphenol content of Himalayan rhubarb, Rheum emodi wall, was measured and the antioxidant activity was determined using DPPH and ABTS+ assay, and the oxidative stress was assessed using SOD enzymatic assay. Five different solvent fractions, n-hexane, n-butanol, ethyl acetate, dichloromethane, and water, were used for screening the antioxidant capacity in effort to determine the optimum extraction solvent. The total phenolic contents for R. emodi fractions ranged from 27.76 to 209.21 mg of gallic acid equivalents (GAE)/g of dry weight. DPPH and ABTS+ assay results are presented into IC50 values, ranged from 21.52 to 2448.79 μg/mL and 90.25 to 1718.05 μg/mL, respectively. The ethyl acetate fraction had the highest antioxidant activity among other fractions. Also, n-butanol and water fractions showed significantly lower IC50 values than the positive control in DPPH radical scavenging activity. The IC50 values of SOD assay of fractions ranged from 2.31 to 64.78 μg/mL. A similar result was observed with ethyl acetate fraction showing the highest SOD radical scavenging activity. The study suggests that the ethyl acetate fraction of R. emodi possess the strongest antioxidant activity, thus the most efficient in extracting antioxidant contents. Moreover, a highly significant correlation was shown between total polyphenol content and antioxidant activity screening assays. The compounds related to the antioxidant activity of R. emodi were identified to myricitrin, myricetin 3-galloyl rhamnoside, and myricetin, which have not been reported in studies about R. emodi before.

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Comparative Assessment of the Basic Chemical Composition and Antioxidant Activity of Stevia rebaudiana Bertoni Dried Leaves, Grown in Poland, Paraguay and Brazil—Preliminary Results

Applied Sciences ◽

10.3390/app11083634 ◽

2021 ◽

Vol 11 (8) ◽

pp. 3634

Author(s):

Teresa Leszczyńska ◽

Barbara Piekło ◽

Aneta Kopeć ◽

Benno F. Zimmermann

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Vitamin C ◽

Dietary Fiber ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Stevia Rebaudiana ◽

Steviol Glycosides ◽

Total Polyphenols ◽

Stevia Rebaudiana Bertoni

This study compares the content of basic nutrients (proteins, fats, digestible carbohydrates, dietary fiber and ash), steviol glycosides, selected antioxidants (vitamin C, total polyphenols) and antioxidant activity in dried leaves of Stevia rebaudiana Bertoni cultivated in Poland, Paraguay and Brazil and available in the direct sale. The basic chemical composition was determined by standard AOAC (Association of Official Analytical Chemists) methods. Content of steviol glycosides was determined by the UHPLC-UV chromatographic method. Total polyphenols content was expressed as gallic acid equivalent (GAE) and catechins equivalent (CE). Antioxidant activity was measured as ABTS●+ free radical scavenging activity. Dried leaves of S. rebaudiana grown in Poland had significantly higher contents of dietary fiber, and lower protein and ash content, compared to those derived from Paraguay and Brazil. The former had, however, considerably higher contents of total steviol glycosides, stevioside and rebaudioside D, compared to the remaining two plants. In the Paraguay-derived dried leaves, the content of rebaudioside A, C, E and rubusoside was found to be significantly lower. Dried leaves of S. rebaudiana Bertoni, cultivated in Poland, contained substantially more vitamin C and a similar content of total polyphenols, compared to those from Brazil and Paraguay. The examined material from Brazil and Paraguay plantations showed similar antioxidant activity, while that obtained from Polish cultivation was characterized by a significantly lower value of this parameter.

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Inhibitory Effect of Antidesma bunius Fruit Extract on Carbohydrate Digestive Enzymes Activity and Protein Glycation In Vitro

Antioxidants ◽

10.3390/antiox10010032 ◽

2020 ◽

Vol 10 (1) ◽

pp. 32

Author(s):

Pattamaporn Aksornchu ◽

Netima Chamnansilpa ◽

Sirichai Adisakwattana ◽

Thavaree Thilavech ◽

Charoonsri Choosak ◽

...

Keyword(s):

Antioxidant Activity ◽

Radical Scavenging Activity ◽

Digestive Enzyme ◽

Radical Scavenging ◽

Protein Glycation ◽

Trolox Equivalent Antioxidant Capacity ◽

Fruit Extract ◽

Antioxidant Power ◽

Ic50 Values

Antidesma bunius (L.) spreng (Mamao) is widely distributed in Northeastern Thailand. Antidesma bunius has been reported to contain anthocyanins, which possess antioxidant and antihypertensive actions. However, the antidiabetic and antiglycation activity of Antidesma bunius fruit extract has not yet been reported. In this study, we investigated the inhibitory activity of anthocyanin-enriched fraction of Antidesma bunius fruit extract (ABE) against pancreatic α-amylase, intestinal α-glucosidase (maltase and sucrase), protein glycation, as well as antioxidant activity. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) chromatogram revealed that ABE contained phytochemical compounds such as cyanidin-3-glucoside, delphinidin-3-glucoside, ellagic acid, and myricetin-3-galactoside. ABE inhibited intestinal maltase and sucrase activity with the IC50 values of 0.76 ± 0.02 mg/mL and 1.33 ± 0.03 mg/mL, respectively. Furthermore, ABE (0.25 mg/mL) reduced the formation of fluorescent AGEs and the level of Nε-carboxymethyllysine (Nε-CML) in fructose and glucose-induced protein glycation during four weeks of incubation. During the glycation process, the protein carbonyl and β-amyloid cross structure were decreased by ABE (0.25 mg/mL). In addition, ABE exhibited antioxidant activity through DPPH radical scavenging activity and Trolox equivalent antioxidant capacity (TEAC) with the IC50 values 15.84 ± 0.06 µg/mL and 166.1 ± 2.40 µg/mL, respectively. Meanwhile, ferric reducing antioxidant power (FRAP) showed an EC50 value of 182.22 ± 0.64 µg/mL. The findings suggest that ABE may be a promising agent for inhibiting carbohydrate digestive enzyme activity, reducing monosaccharide-induced protein glycation, and antioxidant activity.

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