scholarly journals Enhanced Antioxidant Activity under Biomimetic Settings of Ascorbic Acid Included in Halloysite Nanotubes

Antioxidants ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 30 ◽  
Author(s):  
Andrea Baschieri ◽  
Riccardo Amorati ◽  
Tiziana Benelli ◽  
Laura Mazzocchetti ◽  
Emanuele D’Angelo ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Halloysite Nanotubes ◽  
Peroxyl Radicals ◽  
Organic Substrates ◽  
Dpph Radical ◽  
Organic Solution ◽  
Water Ph ◽  
Improved Performance

Antioxidant activity of native vitamin C (ascorbic acid, AH2) is hampered by instability in solution. Selective loading of AH2 into the inner lumen of natural halloysite nanotubes (HNT) yields a composite nanoantioxidant (HNT/AH2), which was characterized and investigated for its reactivity with the persistent 1,1-diphenyl-2-picrylhydrazyl (DPPH•) radical and with transient peroxyl radicals in the inhibited autoxidation of organic substrates, both in organic solution (acetonitrile) and in buffered (pH 7.4) water in comparison with native AH2. HNT/AH2 showed excellent antioxidant performance being more effective than native ascorbic acid by 131% in acetonitrile and 290% (three-fold) in aqueous solution, under identical settings. Reaction with peroxyl radicals has a rate constant of 1.4 × 106 M−1 s−1 and 5.1 × 104 M−1 s−1, respectively, in buffered water (pH 7.4) and acetonitrile, at 30 °C. Results offer physical understanding of the factors governing HNT/AH2 reactivity. Improved performance of HNT/AH2 is unprecedented among forms of stabilized ascorbic acid and its relevance is discussed on kinetic grounds.

scholarly journals In-vitro and In-vivo Antioxidant Activity of Ethanol Leaf Extract of Justicia carnea

2020 ◽  
pp. 48-60
Author(s):  
Udedi Stanley Chidi ◽  
Ani Onuabuchi Nnenna ◽  
Asogwa Kingsley Kelechi ◽  
Maduji Fitzcharles Chijindu ◽  
Okafor Clinton Nebolisa
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Leaf Extract ◽  
Dpph Radical ◽  
Β Carotene ◽  
In Vitro Antioxidant Activity

This study investigated the in-vitro antioxidant activity of ethanol leaf extract of Justicia carnea and its effect on antioxidant status of alloxan-induced diabetic albino rats. The in-vitro antioxidant activity was assayed by determining the total phenol, flavonoids, ascorbic acid, β-carotene and lycopene contents and by using 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical, reducing antioxidant power and inhibition of lipid peroxidation antioxidant systems. Oxidative stress was produced in rats by single intraperitoneal injection of 150 mg/kg alloxan and serum concentration of malonaldehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) were determined. Five experimental groups of rats (n=6) were used for the study. Two groups of diabetic rats received oral daily doses of 100 and 200 mg/kg Justicia carnea leaf extract respectively while gilbenclamide (5 mg/ml); a standard diabetic drug was also given to a specific group for 14 days. From the result, the leaf extract contained a higher concentration of flavonoids followed byphenols, ascorbic acid, lycopene and β-carotene. The extract displayed more potent reducing power ability with EC50 of 40 µg/ml compared to BHA (EC50 of 400µg/ml). The percentage DPPH radical scavenging activity of the extract was also higher with EC50 of 200µg/ml and increased with increase in concentration while BHA had EC50of 320µg/ml. The inhibition of lipid peroxidation also increased with increase in concentration with EC50 of 58µg/ml and comparable with BHA (EC50=60µg/ml). The effect of the plant extract on antioxidant enzyme activities was concentration-dependent. Administration of 100mg/kg of the plant extract resulted in a significant decrease (p<0.05) in serum MDA concentration, while 200 mg/kg of the extract caused a significant (p˂0.05) increase in superoxide dismutase (SOD) and catalase activities with a non-significant increase (p>0.05) in the serum level of MDA when compared with the diabetic untreated group. These findings suggest that ethanol leaf extract of Justicia carnea have antioxidant properties and could handle diabetes-induced oxidative stress.

scholarly journals ESTIMATION OF ANTIOXIDANT PROPERTIES OF 5,10,15,20-TETRAKIS(4-HYDROXYPENYL)PORPHYRINE BASED ON ELECTROCHEMICAL AND BIOLOGICAL RESEARCHES

2019 ◽  
Vol 62 (3) ◽  
pp. 57-63 ◽  
Author(s):  
Sergey S. Kletikov ◽  
Mariya V. Tesakova ◽  
Vladimir I. Parfenyuk ◽  
Vladislav R. Kulagin
Keyword(s):  
Aqueous Solution ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Properties ◽  
Malonic Dialdehyde ◽  
Living Organism ◽  
Considerable Change ◽  
Control Group ◽  
Test Reaction ◽  
Fourth Group

The antioxidant activity of 5,10,15,20-tetrakis(4-hydroxyphenyl)porphyrin was established by a test reaction of interaction with free-radical 1,1-diphenyl-2-picrylhydrazyl (DPPH). A cyclic voltammetry method was used to research the interaction of porphyrin and DPPH. According to the results of the completed research, it was established that H2T(4-OHPh) P has the highest antioxidant activity among all the porphyrins compounds studied by us. The results of the electrochemical experiment served as a basis for testing antioxidant qualities of porphyrin on living creatures – chickens of the “Moscow-black” breed. Chickens were divided into 5 groups: the control group was given the basic ration, the first — the basic ration + 1% ascorbic acid solution, the second — the basic ration + an aqueous solution of Twin-80, the third — the basic ration + an aqueous solution of Twin-80 with porphyrin (10–3 mol/l), the fourth - basic ration + aqueous solution of Twin-80 with porphyrin (10-4 mol/l). In the course of the experiment, the chickens were orally administered the 2 ml solutions studied once a day in for 14 days. Wherein no disturbance of physiological condition of the chickens was noticed, as well as any considerable change of body mass or causes of death. The introduction solution Twin-80 does not impair the blood indexes. In all four experimental groups, a decrease in the concentration of malonic dialdehyde (MDA) was observed compared to the control group: the best result - a decrease in MDA by 56% is observed in the fourth group. In the three groups an increase in serum level of ceruloplasmin was observed. The greatest increase in the concentration of CP also occurs in the fourth group of chickens. With the introduction of ascorbic acid a decrease in CP relative to the control group is not observed. How it was established the 5,10,15,20-tetrakis(4-hydroxyphenyl)porphyrin with concentrations of 10-4 mole/l decline the amount of malonic dialdehyde and increases the amount of ceruloplasmin in blood, which leads to lowering the activity of oxidizing processes in a living organism and also empowers the protein synthesizing function of the liver.

scholarly journals Anti-Inflammatory and Antioxidant Activities of Cordia Dichotoma Forst

2020 ◽  
Vol 13 (4) ◽  
pp. 2093-2099
Author(s):  
Nazim Hussain ◽  
Bibhuti Bhushan Kakoti ◽  
Mithun Rudrapal ◽  
Zubaidur Rahman ◽  
Mokinur Rahman ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Radical Scavenging ◽  
Inflammatory Activity ◽  
Paw Edema ◽  
Dpph Radical ◽  
Standard Drug ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging ◽  
Radical Scavenging Assay

Cordia dichotoma Forst.has been used in the management of pain and inflammations in traditional medicine. However, the anti-inflammatory activity of the methanolic extract of C. dichotoma(MECD) bark has not been reported so far. This work was, therefore, aimed at investigating the anti-inflammatory activity of C. dichotomabark extract. The antioxidant activity was evaluated to justify the anti-inflammatory action of MECD on the basis of its radical scavenging property. The extract of C. dichotomawas obtained by Soxhlation of bark powder using methanol as solvent. The anti-inflammatory activity was determined by the carrageenan induced paw edemamodel in ratsat two different dose levels, viz., 250 and 500 mg/kg. The antioxidant activity was evaluated using DPPH radical scavenging assay. The antioxidant activity was performed in vitro by DPPH radical scavenging assay using ascorbic acid as the standard drug. In anti-inflammatory activity, maximum inhibition of edema was observed after 4 hours of experimental period. At lower test dose (250 mg/kg b.w.), the percentage inhibition of paw edema was 29.7 %, while 48.6 % inhibition of edema was observed at higher dose (500 mg/kgb.w.). The percentage inhibition of paw edema was significant relative to the control group. The standard indomethacin group also exhibited sufficiently high level of anti-inflammatory effect with 56% inhibition of paw edema at 5 mg/kg dose. In in vitroantioxidant activity, the MECD exhibited good DPPHradical scavenging activity with the IC50 vale of 62.46 µg/ml, whereas the standard drug, ascorbic acid showed comparatively more antioxidant activity with IC50 of 27.66 µg/ml.However, our study scientifically validates the folkloric claim as well as traditional uses of C. dichotomaas anti-inflammatory medication. It is suggested that the anti-inflammatory activity of C. dichotomamay be due to the antioxidant potential of phenolic phytoconstituents or plant flavonoids present in the methanolic bark extract.

scholarly journals In vitro antioxidant potential study of some synthetic quinoxalines

2012 ◽  
Vol 38 (2) ◽  
pp. 47-50 ◽  
Author(s):  
MM Hossain ◽  
MM Hossain ◽  
MH Muhib ◽  
MR Mia ◽  
S Kumar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Conventional Heating ◽  
Dpph Radical ◽  
Cyclic Ketones ◽  
Scavenging Effect ◽  
Dpph Method ◽  
Quinoxaline Derivatives ◽  
In Vitro Antioxidant Activity

In continuation of our study the in vitro antioxidant activity of some novel quinoxaline derivatives was investigated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method with respect to ascorbic acid. To determine the antioxidant activity, a number of substituted indoxyls (3A-G), cyclic ketones (2A-G), and quinoxalines (1A-G) were synthesized by both microwave and conventional heating methods. The present findings revealed that some quinoxalines and their precursors (1D, 1F, 1G and 2E) exhibited a marked scavenging effect on DPPH radical. DOI: http://dx.doi.org/10.3329/bmrcb.v38i2.12880 Bangladesh Med Res Counc Bull 2012; 38: 47-50

scholarly journals ANTIOXIDANT ACTIVITY OF THE TOPICAL PREPARATION OF BULUNG SANGU (Gracilaria spp) EXTRACT

2020 ◽  
Vol 7 (2) ◽  
pp. 83
Author(s):  
Maria Malida Vernandes Sasadara ◽  
I Gede Putu Wirawan ◽  
Made Sritamin ◽  
I Ketut Suada ◽  
Wayan Adiartayasa
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Dpph Radical ◽  
Topical Preparation ◽  
Potential Source ◽  
One Way Anova ◽  
Concentration Series ◽  
Acid Percentage

Bulung Sangu (Gracilaria spp) as many other macroalgae, is a source of beneficial phytochemical for health. Phytochemicals in macroalgae are widely used as active ingredients in cosmetic preparations. The study was aimed to determine the antioxidant properties of Bulung Sangu formulated in topical preparation. Bulung Sangu was macerated in ethanol 96% following by extract formulation in cream preparation in two concentrations: 5% and 10%. Antioxidant activity was determined against DPPH radical, compared to ascorbic acid. Percentage of reducing power in five concentration series (10, 20, 30, 40, and 50 µm/ml) statistical analyzes using one-way ANOVA with 95% of confidence level, following by IC50 measurement. Absorbance was read in wavelength of 517 nm. Concentration of 5% and 10% cream extract exhibited IC50 of 13,091 µg/ml and 11,867 µg/ml, respectively. These values are lower than IC50 of ascorbic acid (18,505 µg/ml), indicating the strong antioxidant properties of Bulung Sangu extract cream which is more potential than ascorbic acid. Bulung Sangu exhibit a potential source for being developed as medicine and cosmetics in topical preparation.

scholarly journals New Flavonol Glycoside from the Leaves of Ventilago africana

2015 ◽  
Vol 10 (11) ◽  
pp. 1934578X1501001
Author(s):  
Diane Patricia Apie Gossan ◽  
Abdulmagid Alabdul Magid ◽  
Philomène Akoua Yao-Kouassi ◽  
Damien Le Faucheur ◽  
Antoine Ahibo Coffy ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Radical Scavenging ◽  
2D Nmr ◽  
Positive Control ◽  
Flavonol Glycoside ◽  
Spectroscopic Methods ◽  
Dpph Radical ◽  
Dpph Radical Scavenging ◽  
Radical Scavenging Assay

A new flavonol diglycoside, rhamnocitrin-3- O-α-L-rhamnopyranosyl-(1→6)-β-D-galactopyranoside, was isolated from the leaves of Ventilago Africana. in addition to 11 known flavonoids. Their structures were determined by spectroscopic methods including 1D- and 2D-NMR, and HR-ESI-MS analysis. The isolated compounds were evaluated for their antioxidant activity by using DPPH radical-scavenging assay. Compounds 4, 7–9 have discrete to good antioxidant potential with EC50 values ranging from 20.9 to 40.4 μM. compared with ascorbic acid (EC50 60 μM) used as positive control.

In vitro Antioxidant Activity of a Nigerian Propolis and Its Investigation on Sickle Red Blood Cells

2020 ◽  
Vol 36 (1) ◽  
pp. 61-66
Author(s):  
Chinwe S. Alaribe ◽  
Akolade R. Oladipupo ◽  
Naomi U. Kanu ◽  
Luca Rastrelli
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Sickle Cell Disease ◽  
Red Blood Cells ◽  
Sickle Cell ◽  
Blood Cells ◽  
Dpph Radical ◽  
Cell Disease ◽  
Propolis Extract ◽  
Scavenging Effect

Scientific evidence suggests that antioxidants could reduce the risk of chronic diseases, including cancer and cardiovascular diseases, and play an important role in general wellbeing. Sickle cell disease (SCD) is a debilitating monogenic disorder considered as a public health problem in many countries, but with a major burden in Sub-Saharan Africa. The use of antioxidants could prove relevant and beneficial in the treatment of SCD. In this study, the antioxidant and antisickling activities of methanol extract of a Nigerian propolis were investigated. Antioxidant activity was evaluated by scavenging effect against 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical, and chelating effect against ferrous ions; ascorbic acid and  thylenediamine tetraacetic acid (EDTA) were used, as standards, respectively. Antisickling activity was evaluated using a modified procedure of Emmel’s test, and phosphate buffered saline was used as a negative control. Compared to ascorbic acid (EC50 of 0.02 mg/ml), the propolis extract showed less scavenging effect (EC50 of 0.80 mg/ml) against DPPH radical. The propolis extract (EC50 of 1.84 mg/ml) also showed lower ferrous ion chelating activity compared to EDTA (EC50 of 0.29 mg/ml). In the antisickling assay, micrographic examination showed that, when treated with 1 mg/ml of the propolis extract, the morphology of the sickle red blood cells remained unaffected, thus indicating that the extract did not display antisickling effect at the concentration tested. These results indicate that the propolis extract possess moderate antioxidant activity and could be considered for possible application for its antioxidant benefits. Keywords: Nigerian propolis, oxidative stress, antioxidant activity, sickle cell disease, antisickling activity 

scholarly journals Uji Aktivitas Antioksidan Ekstrak Etanol Kulit Alpukat (Persea americana Mill.) terhadap Peredaman DPPH

2019 ◽  
Vol 10 ◽  
pp. 72-75
Author(s):  
Amelia Siyanti ◽  
Nurul Fitriani ◽  
Angga
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Chemical Compound ◽  
Extraction Method ◽  
Ethanol Extract ◽  
Positive Control ◽  
Persea Americana ◽  
Dpph Radical ◽  
Inhibition Concentration ◽  
Radical Reduction

Avocado peel is contains chemical compound of alkaloid as antioxidant. So, has been research is to determine the antioxidant activity of ethanol extract avocado peel (Persea americana Mill.) inhibition of DPPH radical (2,2-diphenyl-1-picrylhydrazyl). The extraction method used maceration with 96% ethanol solvent. The ethanol extract avocado peel with variant concentrations are 50 ppm, 75 ppm, 100 ppm, 125 ppm, 150 ppm and ascorbic acid standard as a positive control with variant concentrations are 2 ppm, 4 ppm, 6 ppm, 8 ppm, 10 ppm. Antioxidant activity inhibit DPPH (2,2-diphenyl-1-picrylhydrazyl) radical reduction was measured using Uv-Vis spectrophotometry. The results of antioxidant activity was obtained at IC50 (inhibition concentration) from the ethanol extract avocado is 137.34 ppm and ascorbic acid is 13.18 ppm. The results of ethanol extract avocado peel has moderate antioxidant activity.

Uşak İli’nden Toplanan Taxus Baccata (L) Türünün Metanol Ekstresinin Yaprak, Dal ve Meyvesinde Biyolojik Aktivitenin Belirlenmesi

2019 ◽  
Vol 7 (10) ◽  
pp. 1533
Author(s):  
Yasemin Karafakıoglu
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Maximum Amount ◽  
Chemical Components ◽  
Taxus Baccata ◽  
Dpph Radical ◽  
Flavonoid Content ◽  
Future Studies ◽  
Different Parts

Taxus species in different parts of the world prevent the formation of hepatoprotective, anticoagulant, antiulcerogenic, anti-coagulant, antifungal and tumour cells. In this study, biological activity studies were performed on the leaves, branches and fruits of the methanol extract of Taxus baccata L. species collected from Sivaslı district of Uşak province. The methanol extract of the T.baccata species, the total amount of phenolic content of the sample was found to be 14.76 mg GAEQ/1g dry sample in the highest leaves. In T.baccata methanol extract, the maximum amount of flavonoid content was 0.468±mg QE/g dry sample. Antioxidant activity value as DPPH radical; % inhibition value was found as 93.21%. Based on the results; It can be concluded that the leaves of T.baccata have higher antioxidant activity than fruits and branches. Future studies need to explore the chemical components contained in the T.baccata species to determine and investigate in further detail.

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