scholarly journals ESTIMATION OF ANTIOXIDANT PROPERTIES OF 5,10,15,20-TETRAKIS(4-HYDROXYPENYL)PORPHYRINE BASED ON ELECTROCHEMICAL AND BIOLOGICAL RESEARCHES

2019 ◽  
Vol 62 (3) ◽  
pp. 57-63 ◽  
Author(s):  
Sergey S. Kletikov ◽  
Mariya V. Tesakova ◽  
Vladimir I. Parfenyuk ◽  
Vladislav R. Kulagin
Keyword(s):  
Aqueous Solution ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Properties ◽  
Malonic Dialdehyde ◽  
Living Organism ◽  
Considerable Change ◽  
Control Group ◽  
Test Reaction ◽  
Fourth Group

The antioxidant activity of 5,10,15,20-tetrakis(4-hydroxyphenyl)porphyrin was established by a test reaction of interaction with free-radical 1,1-diphenyl-2-picrylhydrazyl (DPPH). A cyclic voltammetry method was used to research the interaction of porphyrin and DPPH. According to the results of the completed research, it was established that H2T(4-OHPh) P has the highest antioxidant activity among all the porphyrins compounds studied by us. The results of the electrochemical experiment served as a basis for testing antioxidant qualities of porphyrin on living creatures – chickens of the “Moscow-black” breed. Chickens were divided into 5 groups: the control group was given the basic ration, the first — the basic ration + 1% ascorbic acid solution, the second — the basic ration + an aqueous solution of Twin-80, the third — the basic ration + an aqueous solution of Twin-80 with porphyrin (10–3 mol/l), the fourth - basic ration + aqueous solution of Twin-80 with porphyrin (10-4 mol/l). In the course of the experiment, the chickens were orally administered the 2 ml solutions studied once a day in for 14 days. Wherein no disturbance of physiological condition of the chickens was noticed, as well as any considerable change of body mass or causes of death. The introduction solution Twin-80 does not impair the blood indexes. In all four experimental groups, a decrease in the concentration of malonic dialdehyde (MDA) was observed compared to the control group: the best result - a decrease in MDA by 56% is observed in the fourth group. In the three groups an increase in serum level of ceruloplasmin was observed. The greatest increase in the concentration of CP also occurs in the fourth group of chickens. With the introduction of ascorbic acid a decrease in CP relative to the control group is not observed. How it was established the 5,10,15,20-tetrakis(4-hydroxyphenyl)porphyrin with concentrations of 10-4 mole/l decline the amount of malonic dialdehyde and increases the amount of ceruloplasmin in blood, which leads to lowering the activity of oxidizing processes in a living organism and also empowers the protein synthesizing function of the liver.

scholarly journals Antioxidant activity test of ethyl acetate fraction of binjai (Mangifera Caesia) leaf ethanol extract

2018 ◽  
Vol 51 (4) ◽  
pp. 164
Author(s):  
K. Khairiah ◽  
Irham Taufiqurrahman ◽  
Deby Kania Tri Putri
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Secondary Metabolite ◽  
Antioxidant Properties ◽  
Linear Connection ◽  
Sampling Technique ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Control Group

Background: Binjai (Mangifera caesia) is a herb derived from South Kalimantan possessing antioxidant properties which promote wound healing inhibiting oxidation radicals. The natural antioxidants present in binjai leaves can be extracted by fractionation. Purpose: This study aimed to analyze the antioxidant activity of ethyl acetate fraction in 96% ethanol extract of binjai leaf. Methods: The study constituted a pure experimental study incorporating a post-test design with only random sampling technique consisting of two groups, namely; an ethyl acetate fraction as the treatment group and ascorbic acid as the positive control group. The leaves were treated in accordance with the soxhlet method and subsequently fractionated to extract ethyl acetate fraction. This was used to measure antioxidant activity with DPPH radical damping method using a UV-Vis spectrophotometer. A linear regression calculation was performed with a standard curve to quantify the IC50 value, before the ethyl acetate fraction underwent a qualitative test of secondary metabolite. Results: An independent t-test indicated significant differences between groups, an average value of IC50 in ascorbic acid of 13.812 ppm with 0.996 linearity and a fraction of ethyl acetate 38.526 ppm with a linearity of 0.999. In contrast, at this linearity value ascorbic acid and ethyl fraction acetate demonstrate a very high linear connection between concentration and inhibition. A secondary metabolite test conducted on the ethyl acetate fraction produced positive results for flavonoid, tannins, and phenol. Conclusion: Based on the IC50 parameters, the fraction of ethyl acetate in 96% ethanol extract of binjai leaf produces very strong antioxidant activity in the content of the compounds in the fraction, namely: flavonoid, tannins and phenol.

Antioxidant properties of 5,10,15,20-tetrakis(4-hydroxypenyl)porphyrine based on electrochemical and biological researches

1970 ◽  
Author(s):  
Sergey Sergeevich Kletikov ◽  
Mariya Vasilyevna Tesakova ◽  
Vladislav Ruslanovich Kulagin ◽  
Mitar Lytovac ◽  
Vladimir Ivanovich Parfenyuk
Keyword(s):  
Antioxidant Activity ◽  
Cyclic Voltammetry ◽  
Free Radical ◽  
Body Mass ◽  
Causes Of Death ◽  
Physiological Condition ◽  
Antioxidant Properties ◽  
Living Organism ◽  
Considerable Change ◽  
Cyclic Voltammetry Method

The evaluation of antioxidant activity of porphyrin was carried out on the mutual reaction of 5,10,15,20-tetrakis(4-hydroxyphenyl)porphyrin with a free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). A cyclic voltammetry method was used to research the interaction of porphyrin and DPPH. The results of the electrochemical experiment served as a basis for testing antioxidant qualities of porphyrin on living creatures – chickens of the “Moscow-black” breed. During the duration of the research, no disturbance of physiological condition of the chickens was noticed, as well as any considerable change of body mass or causes of death. The data on blood’s antioxidant activity lets us evaluate the organism’s ability to defend itself from toxic influences of various oxygen-containing compounds. As data shows, the dilution Twin-80 with porphyrin does not lower the blood condition. During the experiment the following was noted: hydroxyl-containing porphyrin with concentrations of 10-4 mole/l lowers the amount of malondialdehyde and increases the amount of ceruloplasmin in blood, which leads to lowering the activity of oxidizing processes in a living organism, and also empowers the protein synthesizing function of the liver.

VARIETAL FEATURES OF TUBERS OF JERUSALEM ARTICHOKE, GROWING IN THE KRASNODAR REGION AND THE Adygea Republic

2019 ◽  
Author(s):  
Н.Т. Шамкова ◽  
В.Ю. Токарев ◽  
А.В. Добровольская ◽  
А.З. Тодорова ◽  
А.И. ВЕРЕЩАГИНА
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Orotic Acid ◽  
Antioxidant Properties ◽  
Jerusalem Artichoke ◽  
Biotechnological Potential ◽  
Total Sugars ◽  
Krasnodar Region ◽  
Public Catering ◽  
Pectin Substances

Определен биотехнологический потенциал топинамбура сортов Новость ВИР, Скороспелка, Интерес, произрастающего в Краснодарском крае и Республике Адыгея, урожаев 2011 2018 гг. Установлено, что клубни топинамбура сорта Интерес превосходят сорта Новость ВИР и Скороспелка по содержанию, : общих сахаров на 22,41 и 14,34 инулина 41,82 и 32,99 пектиновых веществ 22,39 и 49,75 аскорбиновой кислоты 35,27 и 39,29 хлорогеновой кислоты 3,66 и 18,29 оротовой кислоты 88,89 и 84,89 соответственно. Сорт Интерес в сравнении с сортами Новость ВИР и Скороспелка содержит на 245,24 и 243,33 меньше никотиновой кислоты и на 100,0 и 108,33 кофейной кислоты соответственно. Содержание гистидина в клубнях топинамбура сортов Интерес, Новость ВИР и Скороспелка составляет 132,36 44,83 и 88,21 мг/кг аргинина 2017,2 958,56 и 460,44 мг/кг соответственно. В комплексе с аскорбиновой кислотой органические кислоты клубней топинамбура обладают выраженными антиоксидантными свойствами. Установлено, что в результате бланширования антиоксидантная активность топинамбура сорта Интерес возросла на 15,6. Таким образом, сорт Интерес наиболее перспективен для переработки в условиях предприятий общественного питания. The biotechnological potential of Jerusalem artichoke varieties Novost VIR, Skorospelka, Interes growing in the Krasnodar region and the Adygea Republic, harvest 2011 2018 has been determined. It was found that Jerusalem artichoke tubers of the Interes variety are superior to Novost VIR and Skorospelka varieties in content, : total sugars by 22,41 and 14,34 inulin 41,82 and 32,99 pectin substances 22,39 and 49,75 ascorbic acid 35,27 and 39,29 chlorogenic acid 3,66 and 18,29 orotic acid 88,89 and 84,89, respectively. Variety Interes in comparison with Novost VIR and Skorospelka varieties contains 245,24 and 243,33 less nicotinic acid and 100,00 and 108,33 coffee acid, respectively. The content of histidine in the tubers of Jerusalem artichoke varieties of Interes, Novost VIR, Skorospelka is 132,36 44,83 and 88,21 mg/kg arginine 2017,2 958,56 and 460,44 mg/kg, respectively. In a complex with ascorbic acid, organic acids of Jerusalem artichoke tubers have pronounced antioxidant properties. It was found that as a result of blanching, the antioxidant activity of Jerusalem artichoke of the Interes variety increases by 15,6. Thus, the Interes variety is most promising for processing in public catering facilities.

scholarly journals THE EFFECTIVENES OF COMPLEX DRUG “BIFIDUM BAG” FOR STATUS CORRECTION OF LARGE INTESTINE MICROBOICENOSIS AND ANTIOXIDANT PROPERTIES OF COLONOCYTES IN EXPERIMENTAL DYSBIOSIS

2019 ◽  
Vol 23 (2) ◽  
pp. 203-210
Author(s):  
O. A. Medvedeva ◽  
V. A. Korolev ◽  
N. A. Verevkina ◽  
V. A. Riadnova
Keyword(s):  
Superoxide Dismutase ◽  
Large Intestine ◽  
Antioxidant Properties ◽  
Malonic Dialdehyde ◽  
The State ◽  
Control Group ◽  
Experimental Animals ◽  
Bacteriological Method ◽  
Complex Preparation ◽  
Complex Drug

The effectiveness of complex drug “Bifidum BAG” for status correction of large intestine microbiocenosis and antioxidant properties of colonocytes in experimental dysbiosis has been studied. Acomplex drug was administered to experimental animals, which includes Bifidobacterium and Dihydroquercetin. Quantitative and qualitative study of large intestine was done in mices by bacteriological method. The state of lipid peroxidation system was evaluatedaccoding to the content of acylhydroperoxide and malonic dialdehyde. The state of antioxidant protective system was reached by means of catalase and superoxide dismutase activity. Experimental dysbiosis was shown as significant changes in mucosal microflora, changes colonocytes antioxidant properties. The use of the complex preparation “Bifidum BAG”, led to the normalization of the colon microbiota (11 of 16 microorganisms were recovered). After correction gentamicin-associated dysbiosis with a complex probiotic, a positive effect of the drug on the colonocytes antioxidant defense was noted. So the activity of catalase increased 1.1 times, compared with the determined index in the group “dysbiosis”. The activity of superoxide dismutase increased 2 times in comparison with the group “dysbiosis”, exceeding the value of the control group. The concentration of LPO products in colonocytes of experimental animals decreased significantly. The content of malonicdialdehyde and acylhydroperoxide decreased 1.6 times and 5.6 times in comparison with the determined index of the group “dysbiosis”, respectively.

scholarly journals Antioxidant Activities of Extracts from Celery Leaves (Apium Graveolens L) Grown in Jos, Nigeria

2020 ◽  
pp. 1-5
Author(s):  
M. Suleman Stephen ◽  
E. A. Adelakun ◽  
J. H. Kanus ◽  
Meshack M. Gideon
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Acetone Extract ◽  
Apium Graveolens ◽  
Isolation And Identification ◽  
Celery Plant

The presence of natural antioxidant in plants is well known. Plant phenolics constitute one of the major groups of components that act as antioxidant and free radical terminator. Hence, this study focused on investigating the antioxidant activity of Celery plant (Apium graveolens L). The fresh leaves were collected, crushed and extracted with ethanol and acetone by maceration. The radical scavenging properties of the extracts were determined by measuring changes in absorbance of DPPH radical at a wave lenght of 517 nm by UV and ascorbic acid is used as the standard. It showed that the crude ethanolic extract has higher antioxidant activity compared to ascorbic acid and acetone extract with less scavenging activity. The values were (IC50 114.6 µg/mL) for ascorbic acid, (IC50 112 µg/mL) for the crude ethanolic extract and (IC50172 µg/mL) for crude acetone extract. The result shows that Celery plant grown in Jos possess good antioxidant properties which may be linked to the presence of phenolics and flavonoids in the plant, which justifies its use as a medicinal plant. This can be further investigated for the isolation and identification of active compounds of medicinal utilities.

scholarly journals In VivoAntioxidant Activity of Deacetylasperulosidic Acid in Noni

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
De-Lu Ma ◽  
Mai Chen ◽  
Chen X. Su ◽  
Brett J. West
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Catalase Activity ◽  
Antioxidant Properties ◽  
Superoxide Dismutase Activity ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Dose Dependent

Deacetylasperulosidic acid (DAA) is a major phytochemical constituent ofMorinda citrifolia(noni) fruit. Noni juice has demonstrated antioxidant activityin vivoand in human trials. To evaluate the role of DAA in this antioxidant activity, Wistar rats were fed 0 (control group), 15, 30, or 60 mg/kg body weight per day for 7 days. Afterwards, serum malondialdehyde concentration and superoxide dismutase and glutathione peroxidase activities were measured and compared among groups. A dose-dependent reduction in malondialdehyde was evident as well as a dose-dependent increase in superoxide dismutase activity. DAA ingestion did not influence serum glutathione peroxidase activity. These results suggest that DAA contributes to the antioxidant activity of noni juice by increasing superoxide dismutase activity. The fact that malondialdehyde concentrations declined with increased DAA dose, despite the lack of glutathione peroxidase-inducing activity, suggests that DAA may also increase catalase activity. It has been previously reported that noni juice increases catalase activityin vivobut additional research is required to confirm the effect of DAA on catalase. Even so, the current findings do explain a possible mechanism of action for the antioxidant properties of noni juice that have been observed in human clinical trials.

scholarly journals In vitro antioxidant activity of meniran (Phyllantus urinaria) functional drink in human low density lipoprotein (LDL)

2021 ◽  
Vol 913 (1) ◽  
pp. 012093
Author(s):  
U Fitrotin ◽  
N Hilmiati ◽  
Mardiana ◽  
Y Triguna ◽  
A Surahman ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Density Lipoprotein ◽  
Ldl Oxidation ◽  
Functional Drink

Abstract Preparation process for meniran (Phillantus urinaria) functional drink (MFD) influences its antioxidant activity. This research aims to understand the phenolic content, DPPH Radical Scavenging Activity (RSA), and LDL oxidation of MFD through various preparation processes. Those preparation processes included soaking fresh meniran (SFM), boiling fresh meniran for 5 minutes (BFM5’), boiling fresh meniran for 10 minutes (BFM10’), and soaking dried meniran (DM). The phenolic content was determined with Folin–Ciocalteu, antioxidant activity was assessed using DPPH and TBARS assay with LDL as the oxidation substrate. An antioxidant references in this research used ascorbic acid. The phenolic content in methods of SFM, BFM5’, BFM10’ and DM were 122±0.022, 182±0.043, 192 ±0.03, and 117 ±0.019 mg GAE/g of meniran respectively. Meanwhile, the DPPH RSA of SFM, BFM5’, BFM10’ and DM accounted for 82.18±0.35, 86.19±0.53, 86.75±0.64 and 69.96% respectively. As comparison, the DPPH RSA of ascorbic acid 50 ppm is 75.65±0.82%. At the same time the optimum inhibition of TBARS formation from BFM5’ and BFM10’ methods were 45.83 % and 48.66%, with MDA concentration in human LDL accounted for 38.30±2.39 and 36.30±1.82 nmol MDA/mg protein, respectively. As comparison, MDA concentration in human LDL added with ascorbic acid 25 ppm accounted for 41.35±2.41 nmol MDA/mg protein. In contrast, the control human LDL was 70.70±2.35 nmol MDA/mg protein. This study concludes that the BFM5’ and BFM10’ methods showed the highest antioxidant properties compared to other methods. All methods showed that MFD extract in concentration more than 25 ppm increased the concentration of MDA in human LDL. Therefore, to produce meniran functional drink in optimum antioxidant properties is best by using BFM5’ and BFM10’ preparation methods in meniran concentration of not more than 25 ppm.

scholarly journals Habitat Affects the Chemical Profile, Allelopathy, and Antioxidant Properties of Essential Oils and Phenolic Enriched Extracts of the Invasive Plant Heliotropium Curassavicum

Plants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 482 ◽  
Author(s):  
Ahmed M. Abd-ElGawad ◽  
Abdelsamed I. Elshamy ◽  
Saud L. Al-Rowaily ◽  
Yasser A. El-Amier
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Essential Oils ◽  
Invasive Plant ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Bioactive Constituents ◽  
Chenopodium Murale ◽  
Two Samples ◽  
Ic50 Values

The variation in habitat has a direct effect on the plants and as a consequence, changes their content of the bioactive constituents and biological activities. The present study aimed to explore the variation in the essential oils (EOs) and phenolics of Heliotropium curassavicum collected from the coastal and inland habitats. Additionally, we determined their antioxidant and allelopathic activity against the weed, Chenopodium murale. Fifty-six compounds were identified as overall from EOs, from which 25 components were identified from the coastal sample, and 52 from the inland one. Sesquiterpenes were the main class in both samples (81.67% and 79.28%), while mono (3.99% and 7.21%) and diterpenes (2.9% and 1.77%) represented minors, respectively. Hexahydrofarnesyl acetone, (-)-caryophyllene oxide, farnesyl acetone, humulene oxide, farnesyl acetone C, and nerolidol epoxy acetate were identified as major compounds. The HPLC analysis of MeOH extracts of the two samples showed that chlorogenic acid, rutin, and propyl gallate are major compounds in the coastal sample, while vanilin, quercetin, and 4′,7-dihydroxyisoflavone are majors in the inland one. The EOs showed considerable phytotoxicity against C. murale with IC50 value of 2.66, 0.59, and 0.70 mg mL−1 for germination, root, and shoot growth, respectively from the inland sample. While the coastal sample attained the IC50 values of 1.58, 0.45, and 0.66 mg mL−1. MeOH extracts revealed stronger antioxidant activity compared to the EOs. Based on IC50 values, the ascorbic acid revealed 3-fold of the antioxidant compared to the EO of the coastal sample and 4-fold regarding the inland sample. However, the ascorbic acid showed 3-fold of the antioxidant activity of the MeOH extracts of coastal and inland samples. Although H. curassavicum is considered as a noxious, invasive plant, the present study revealed that EO and MeOH extracts of the H. curassavicum could be considered as promising, eco-friendly, natural resources for antioxidants as well as weed control, particularly against the weed, C. murale.

scholarly journals Enhanced Antioxidant Activity under Biomimetic Settings of Ascorbic Acid Included in Halloysite Nanotubes

Antioxidants ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 30 ◽  
Author(s):  
Andrea Baschieri ◽  
Riccardo Amorati ◽  
Tiziana Benelli ◽  
Laura Mazzocchetti ◽  
Emanuele D’Angelo ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Halloysite Nanotubes ◽  
Peroxyl Radicals ◽  
Organic Substrates ◽  
Dpph Radical ◽  
Organic Solution ◽  
Water Ph ◽  
Improved Performance

Antioxidant activity of native vitamin C (ascorbic acid, AH2) is hampered by instability in solution. Selective loading of AH2 into the inner lumen of natural halloysite nanotubes (HNT) yields a composite nanoantioxidant (HNT/AH2), which was characterized and investigated for its reactivity with the persistent 1,1-diphenyl-2-picrylhydrazyl (DPPH•) radical and with transient peroxyl radicals in the inhibited autoxidation of organic substrates, both in organic solution (acetonitrile) and in buffered (pH 7.4) water in comparison with native AH2. HNT/AH2 showed excellent antioxidant performance being more effective than native ascorbic acid by 131% in acetonitrile and 290% (three-fold) in aqueous solution, under identical settings. Reaction with peroxyl radicals has a rate constant of 1.4 × 106 M−1 s−1 and 5.1 × 104 M−1 s−1, respectively, in buffered water (pH 7.4) and acetonitrile, at 30 °C. Results offer physical understanding of the factors governing HNT/AH2 reactivity. Improved performance of HNT/AH2 is unprecedented among forms of stabilized ascorbic acid and its relevance is discussed on kinetic grounds.

scholarly journals Antioxidant potential study of some synthesized N-heterocycles

2009 ◽  
Vol 35 (2) ◽  
pp. 49-52 ◽  
Author(s):  
Mohammad Mamun Hossain ◽  
Sukanta Kumar Shaha ◽  
Foysal Aziz
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Anticancer Activity ◽  
Heterocyclic Compounds ◽  
Antioxidant Properties ◽  
Biological Properties ◽  
Antioxidant Potential ◽  
Heating Method ◽  
Dpph Method ◽  
First Time

Nitrogen containing heterocyclic compounds such as oxindoles especially isatins and their derivatives have excellent biological properties such as anti-inflammatory, antimicrobial, anticancer activity. In addition, this is first time ever we would like to report the antioxidant properties of the said class of compounds. To determine such an important bioactivity a number of halogenated oxindoles 1-3, their diaza-biphenyl derivatives (4-6), ring extended biphenyl (7) and bis-amide (8) were synthesized by classical heating method. The antioxidant activity of all the synthesized compounds was screened by DPPH method with respect to ascorbic acid. In our present investigation some of the synthesized compounds (1, 2, 3, 5 and 6) were found to be active.Keywords: Antioxidant; Isatin; OxindoleOnline 12 August 2009DOI: 10.3329/bmrcb.v35i2.2564Bangladesh Med Res Counc Bull 2009; 35: 49-52 

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