scholarly journals Protective Effect of Selected Antioxidants on Naproxen Photodegradation in Aqueous Media

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 424 ◽  
Author(s):  
Kohei Kawabata ◽  
Ayaka Takato ◽  
Sayaka Oshima ◽  
Shiori Akimoto ◽  
Masanori Inagaki ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Protective Effect ◽  
Uv Irradiation ◽  
High Performance ◽  
Antioxidative Activity ◽  
Aqueous Media ◽  
Reducing Power ◽  
Protective Effects ◽  
Residual Rate ◽  
Effective Antioxidant

A photostabilization strategy is an important aspect of quality assurance for photosensitive compounds. This study focused on the photoprotective effects of selected antioxidants including the effect of L-ascorbic acid (AA) on naproxen (NX) photodegradation in aqueous media. NX degradation during ultraviolet light (UV) irradiation and the protective effects of selected antioxidants were monitored by high-performance liquid chromatography (HPLC). The addition of AA induced the suppression of NX photodegradation, although the protective effect disappeared after AA was degraded completely. The results of the evaluations on the photoprotective effects on NX photodegradation and antioxidative activities of AA and other antioxidants showed that the protective effects of antioxidants are dependent on reducing power and photostability under UV irradiation. In this experiment, quercetin (QU) is the most effective antioxidant on account of the residual rate of QU after UV irradiation and the antioxidative activity in the potential antioxidant (PAO) test was significantly higher compared to other antioxidants following the higher protective effect on NX photodegradation.

scholarly journals Photoprotective Effects of Selected Amino Acids on Naproxen Photodegradation in Aqueous Media

2020 ◽  
Vol 13 (6) ◽  
pp. 135
Author(s):  
Kohei Kawabata ◽  
Momoka Kanoh ◽  
Mayu Okazaki ◽  
Rina Maeda ◽  
Satomi Mori ◽  
...  
Keyword(s):  
Amino Acids ◽  
Uv Irradiation ◽  
High Performance ◽  
Model Compound ◽  
Aqueous Media ◽  
Protective Effects ◽  
Residual Amount ◽  
Uv Absorption Spectrum ◽  
Antioxidative Ability

It is important to develop a photostabilization strategy to ensure the quality of photosensitive compounds, including pharmaceuticals. This study focused on the protective effects of 20 amino acids on the photodegradation of naproxen (NX), a photosensitive pharmaceutical, to clarify the important nature of a good photostabilizer. Our previous report indicated the photodegradability of NX and the protective effects of some antioxidants on its photodegradation, therefore, this compound was used as a model compound. The degradation of NX in aqueous media during ultraviolet light (UV) irradiation and the protective effects of selected amino acids were monitored through high-performance liquid chromatography (HPLC), equipped with a reverse-phase column. Addition of cysteine, tryptophan, and tyrosine induced the significant suppression of NX photodegradation after UV irradiation for 3 h (residual amount of NX; 15.35%, 6.82%, and 15.64%, respectively). Evaluation of the antioxidative activity and UV absorption spectrum showed that cysteine suppressed NX degradation through its antioxidative ability, while tryptophan and tyrosine suppressed it through their UV filtering ability. Furthermore, three amino acids at higher concentrations (more than 100 µmol/L) showed more protective effects on NX photodegradation. For 10 mmol/L, residual amounts of NX with cysteine, tryptophan, and tyrosine were 58.51%, 69.34%, and 82.40%, respectively. These results showed the importance of both photoprotective potencies (antioxidative potency and UV filtering potency) and stability to UV irradiation for a good photostabilizer of photosensitive pharmaceuticals.

scholarly journals HPLC-PDA Simultaneous Determination and Protective Effect of Anemarrhena asphodeloides Against Acute Renal Failure

2014 ◽  
Vol 9 (6) ◽  
pp. 1934578X1400900
Author(s):  
Chang-Seob Seo ◽  
Hyekyung Ha ◽  
Young-Jung Kim ◽  
Ju-Young Jung
Keyword(s):  
Renal Failure ◽  
Acute Renal Failure ◽  
Acetic Acid ◽  
Protective Effect ◽  
High Performance ◽  
Glutathione Depletion ◽  
Aqueous Acetic Acid ◽  
Protective Effects ◽  
Relative Standard ◽  
Anemarrhena Asphodeloides

We investigated the protective effects against acute renal failure (ARF) of Anemarrhena asphodeloides (AA) and performed simultaneous analysis of three compounds, neomangiferin (1), mangiferin (2), and isomangiferin (3) in AA using a high-performance liquid chromatography-photodiode array. To measure the protective effect of ARF, the levels of reactive oxygen species (ROS) and glutathione depletion were determined using a kit. HPLC analysis was performed using a Gemini C18 column at 40°C. The mobile phase used gradient elution with 1.0% (v/v) aqueous acetic acid (A) and 1.0% (v/v) acetic acid in acetonitrile (B). The flow rate was 1.0 mL/min. In our assay, AA extract inhibits cisplatin-induced production of intracellular ROS. Pre-incubation of AA extracts (10–200 μg/mL) markedly maintained cell viability compared with controls in the noncisplatin-treated cells. Calibration curves of all compounds showed good linearity ( r2  0.9992). Recoveries of the three compounds were 98.9–103.4%. The relative standard deviations of intra- and inter-day precision were 0.07–1.73% and 0.12–1.49%, respectively. The amounts of the three components were 1.22–20.63 mg/g. The AA extract has potential as a therapeutic agent for treatment of ARF. In addition, the established method will help to improve quality control of AA.

scholarly journals Protective Effect of D-Methionine on Body Weight Loss, Anorexia, and Nephrotoxicity in Cisplatin-Induced Chronic Toxicity in Rats

2018 ◽  
Vol 17 (3) ◽  
pp. 813-824 ◽  
Author(s):  
Ming-Tai Lin ◽  
Jiunn-Liang Ko ◽  
Te-Chung Liu ◽  
Pei-Tsen Chao ◽  
Chu-Chyn Ou
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Protective Effect ◽  
Antioxidative Activity ◽  
Body Weight Loss ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Protective Effects ◽  
Feeding Efficiency

D-methionine is a sulfur-containing amino acid that can act as a potent antioxidant. Anorexia and nephrotoxicity are side effects of cisplatin. The protective effects of D-methionine on cisplatin-induced anorexia and renal injury were investigated. The model of chronic cisplatin administration (5 mg/kg body weight) involved intraperitoneal injection on days 1, 8, and 15 and oral D-methionine (300 mg/kg body weight) coadministration daily for 20 days. On the 21st day of treatment, food intake and body weight in the cisplatin-treated group significantly decreased by 52% and 31%, respectively, when compared with a control group. D-methionine coadministration with cisplatin decreased food intake and body weight by 29% and 8%, respectively. In cisplatin-treated rats, white blood cell, mean corpuscular volume, and platelet values significantly decreased, while mean corpuscular hemoglobin concentration significantly increased by 8.6% when compared with control rats. Cisplatin administration resulted in significantly decreased feeding efficiency, elevated renal oxidative stress, and reduced antioxidative activity. Leukocyte infiltration, tubule vacuolization, tubular expansion, and swelling were observed in the kidneys of cisplatin-treated rats. Oral D-methionine exhibited an antianorexic effect, with improvement in food intake, feeding efficiency, and hematological toxicities, as well as a protective effect against nephrotoxicity by elevated antioxidative activity. D-methionine may serve as a chemoprotectant in patients receiving cisplatin as part of a chemotherapy regimen.

Influence of pH on the Antioxidative Activity of Maillard Reaction Products Generation from Model Reaction of L-Ascorbic Acid and L-Lysine/L-Methionine

2013 ◽  
Vol 634-638 ◽  
pp. 397-402
Author(s):  
Qi Hui Deng ◽  
Ai Nong Yu
Keyword(s):  
Ascorbic Acid ◽  
Maillard Reaction ◽  
Antioxidative Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Model Systems ◽  
Maillard Reaction Products ◽  
Reaction Products ◽  
Intermediate Products ◽  
Initial Ph

Maillard reaction products (MRPs) were prepared by heating the solution containing L-ascorbic acid (ASA)and L-lysine (Lys)/ L-methionine (Met) adjusted to various pHs (4.0, 5.0, 6.0, 7.0 and 8.0) at 140±2°C for 2 h. The browning, intermediate products, reducing power and DPPH (1,1-diphenyl-2-picryl-hydrazyl) radical-scavenging activity reached a maximum with initial pH 5.0 (P < 0.05). Thereafter, slightly differences with initial pH of 7.0 and 8.0 were found. An increase in Ferrous ion chelating activity of MRPs derived from ASA-Lys/ Met model systems, as measured by absorbance at 562 nm, was observed as the initial pH increased (P < 0.05). In general , antioxidative activity of ASA-Lys/ Met MRPs was coincidental with the browning development and the formation of intermediate products.

scholarly journals THE COMPOSITION AND ANTIOXIDATIVE ACTIVITY OF SPRUCE ROOTS’ EXTRACT

2020 ◽  
pp. 195-202
Author(s):  
Dmitriy Konstantinovich Gulyaev ◽  
Valentina Dmitriyevna Belonogova ◽  
Dmitriy Olegovich Bokov ◽  
Vladimir Vladimirovich Bessonov
Keyword(s):  
Ascorbic Acid ◽  
High Performance ◽  
Antioxidative Activity ◽  
Raw Materials ◽  
Water Extract ◽  
Hydroxycinnamic Acids ◽  
Water Soluble ◽  
Biologically Active ◽  
Soluble Form ◽  
Phenol Compounds

The spruce Picea abies (L.) (Pinaceae) roots are logging residues and are not used nowadays. However, spruce roots are a source of valuable biologically active substances that have potential for medical use. The purpose is to research the composition of phenol compounds and antioxidative activity of spruce roots water extract. The raw materials for the research were the spruce roots collected on a cutting area on the day of sawing a tree. Water extract was obtained from the roots, and then polysaccharides were removed from it. Composition of the extract phenol compounds was determined using a high performance liquid chromatography on the chromatograph Agilent 1100. Compounds identification was based on retention times. Extract’s antioxidative activity was determined by the reaction with the stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH), the EC50 value computed. Ascorbic acid and trolox (a water-soluble form of vitamin E) were used as standard substances. As a result, it was found that flavonoids, hydroxycinnamic acids, phenolcarboxylic acids are present in the spruce roots water extract. Ferulic acid has the highest amount amongst hydroxycinnamic acids in the spruce roots water extract, hyperoside – amongst flavonoids. It was found that the spruce roots water extract, without polysaccharides, has a pronounced antioxidative activity similar in strength to ascorbic acid, which allows us to talk about the prospects for further researches and the medicinal drugs manufacturing.

Antioxidant Activity, Phenolic Content and Hematoprotective Effects of Cleome arabica Polyphenolic Leaf Extract

2019 ◽  
Author(s):  
C. Tigrine ◽  
A. Kameli
Keyword(s):  
Antioxidant Activity ◽  
Biological Effects ◽  
Reducing Power ◽  
Hematological Toxicity ◽  
Protective Effects ◽  
Ferrous Ions ◽  
Polyphenolic Extract ◽  
Cleome Arabica

In this study a polyphenolic extract from Cleome arabica leaves (CALE) was investigated for its antioxidant activity in vitro using DPPH•, metal chelating and reducing power methods and for its protective effects against AraC-induced hematological toxicity in vivo using Balb C mice. Results indicated that CALE exhibited a strong and dose-dependent scavenging activity against the DPPH• free radical (IC50 = 4.88 μg/ml) and a high reducing power activity (EC50 = 4.85 μg/ml). Furthermore, it showed a good chelating effects against ferrous ions (IC50 = 377.75 μg/ml). The analysis of blood showed that subcutaneous injection of AraC (50 mg/kg) to mice during three consecutive days caused a significant myelosupression (P < 0.05). The combination of CALE and AraC protected blood cells from a veritable toxicity. Where, the number of the red cells, the amount of hemoglobin and the percentage of the hematocrite were significantly high. On the other hand, AraC cause an elevation of body temperature (39 °C) in mice. However, the temperature of the group treated with CALE and AraC remained normal and did not exceed 37.5 °C. The observed biological effects of CALE, in vitro as well as in vivo, could be due to the high polyphenol and flavonoid contents. In addition, the antioxidant activity of CALE suggested to be responsible for its hematoprotective effect.

The Influence of Some Factors on Spirolactone Stability in Solution

2008 ◽  
Vol 59 (7) ◽  
Author(s):  
Daniela Lucia Muntean ◽  
Silvia Imre ◽  
Cosmina Voda
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Uv Irradiation ◽  
High Performance ◽  
Degradation Products ◽  
Simultaneous Action ◽  
Ethanolic Solution ◽  
Degradation Processes ◽  
Stable Solutions ◽  
Preformulation Study

The influence of some factors on spironolactone stability in solution was studied, by applying high-performance liquid chromatography, as a part of a pharmaceutical preformulation study in order to obtain a spironolactone solution for alopecia treatment. Solutions of 1 mg/ml spironolactone in aqueous ethanolic solution 1 : 1 and in 20 mM cyclodextrines solutions (b-, hydroxi-b- and methyl-b-cyclodextrine) was used, maintained at 8 and 22 �C, protected from light and after UV irradiation at 254 nm. The main degradation products were 7a-thiospirolactone and canrenone. The most stable solutions were the alcoholic ones and with methyl-beta-cyclodextrine, but the simultaneous action of temperature and UV irradiation allowed degradation processes after one hour of exposure, more aggressive in the presence of methyl-beta-cyclodextrine. In conclusion, for alopecia treatment with spironolactone a 1 mg/mL ethanolic solution could be used and it is recommendable the protection of treated zone.

The Protective Effect of Metformin against Oxandrolone-Induced Infertility in Male Rats

2020 ◽  
Vol 26 ◽  
Author(s):  
Abdulqader Fadhil Abed ◽  
Yazun Bashir Jarrar ◽  
Hamzeh J Al-Ameer ◽  
Wajdy Al-Awaida ◽  
Su-Jun Lee
Keyword(s):  
Gene Expression ◽  
Male Infertility ◽  
Protective Effect ◽  
Histological Examination ◽  
Sperm Count ◽  
Serum Testosterone ◽  
Male Rats ◽  
P Value ◽  
Protective Effects ◽  
Rt Pcr

Background: Oxandrolone is a synthetic testosterone analogue that is widely used among bodybuilders and athletes. However, oxandrolone causes male infertility. Recently, it was found that metformin reduces the risk of infertility associated with diabetes mellitus. Aim: This study aimed to investigate the protective effects of metformin against oxandrolone-induced infertility in male rats. Methods: Rats continuously received one of four treatments (n=7) over 14 days: control DMSO administration, oxandrolone administration, metformin administration, or co-administration of oxandrolone and metformin. Doses were equivalent to those used for human treatment. Subsequently, testicular and blood samples were collected for morphological, biochemical, and histological examination. In addition, gene expression of the testosterone synthesizing enzyme CYP11A1 was analyzed in the testes using RT-PCR. Results: Oxandrolone administration induced male infertility by significantly reducing relative weights of testes by 48%, sperm count by 82%, and serum testosterone levels by 96% (ANOVA, P value < 0.05). In addition, histological examination determined that oxandrolone caused spermatogenic arrest which was associated with 2-fold downregulation of testicular CYP11A1 gene expression. However, co-administration of metformin with oxandrolone significantly ameliorated toxicological alterations induced by oxandrolone exposure (ANOVA, P value < 0.05). Conclusion: Metformin administration protected against oxandrolone-induced infertility in male rats. Further clinical studies are needed to confirm the protective effect of metformin against oxandrolone-induced infertility among athletes.

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