An Environmentally Friendly Practice Used in Olive Cultivation Capable of Increasing Commercial Interest in Waste Products from Oil Processing

In the Rural Development Plan (2014–2020), the European Commission encouraged the conversion and supported the maintenance of organic farming. Organic olive oil (bioEVOO) production involves the use of environmentally sustainable fertilizers and the recycling of olive pomace (Pom) and olive vegetation waters (VW) to reduce the environmental impact of these wastes. An ecofriendly way to recycle olive wastes is to reuse them to extract bioactive compounds. In this study, the total phenolic compounds content, their profile and dosage, the antioxidant action in oil, pomace, and vegetation water was evaluated when the Trichoderma harzianum M10 was used as a biostimulant in agriculture. Two spectrophotometric tests (2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic) acid (ABTS)) evaluated the antioxidant potential of samples, a spectrophotometric method estimated total phenolic content, and an Ultra-High-Performance Liquid Chromatography (UHPLC)–Orbitrap method evaluated the phenolics profile. Our results showed that the biostimulation improved the antioxidant potential and the total concentration of phenolics in the bioEVOO and bio-pomace (bioPom) samples and mainly enhanced, among all classes of phenolic compounds, the production of the flavonoids and the secoiridoids. Moreover, they demonstrated the Trichoderma action in the mevalonate pathway to produce phenols for the first time. The decisive action of the Thricoderma on the production of phenolic compounds increases the economic value of the waste materials as a source of bioactive compounds useful for the pharmaceutical, cosmetic, and food industries.

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Seasonal Variation of Health-Promoting Bioactives in Broccoli and Methyl-Jasmonate Pre-Harvest Treatments to Enhance Their Contents

Foods ◽

10.3390/foods9101371 ◽

2020 ◽

Vol 9 (10) ◽

pp. 1371 ◽

Cited By ~ 1

Author(s):

Vanesa Nuñez-Gómez ◽

Nieves Baenas ◽

Inma Navarro-González ◽

Javier García-Alonso ◽

Diego A. Moreno ◽

...

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Phenolic Compounds ◽

Methyl Jasmonate ◽

Bioactive Compounds ◽

High Performance ◽

Total Phenolic ◽

Diode Array ◽

Total Carotenoids ◽

Health Promoting

Broccoli is a source of bioactive compounds that provide an important nutritional value. The content of these compounds can vary depending on agronomic and environmental conditions, as well as on elicitation. In this study, three crop trials were carried out to evaluate the effects of the cultivation season, the application of different dosages of methyl-jasmonate (MeJA) on the overall quality and on the total content of bioactive compounds of ‘Parthenon’ broccoli cultivated under the field conditions of southeastern Spain. Color parameters, chlorophyll content, total phenolic compounds, total flavonoids and antioxidant activity were measured to evaluate the overall quality. Moreover, individual carotenoids, phenolic compounds and glucosinolates were evaluated by high performance liquid chromatography with diode array detection (HPLC-DAD) and high performance liquid chromatography equipped with diode array detector coupled to mass spectrometer using electro spray ionization (HPLC-DAD-ESI/MSn). The content of total carotenoids, phenolic compounds and glucosinolates were higher in autumn compared with spring, showing increases of 2.8-fold, 2-fold and 1.2-fold, respectively. Moreover, a double application of MeJA increased the contents of total carotenoids, phenolic compounds and glucosinolates by 22%, 32% and 39%, respectively, relative to the untreated samples. Considering our results, the controlled and timely application of 250 µM MeJA to the aerial parts of the plants four days before harvest, on two consecutive days, seems to be a valid agronomic strategy to improve the health-promoting capacity of Parthenon broccoli, without compromising its overall quality.

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Myrtus communis Liquor Byproduct as a Source of Bioactive Compounds

Foods ◽

10.3390/foods8070237 ◽

2019 ◽

Vol 8 (7) ◽

pp. 237 ◽

Cited By ~ 4

Author(s):

Correddu ◽

Maldini ◽

Addis ◽

Petretto ◽

Palomba ◽

...

Keyword(s):

Phenolic Compounds ◽

Bioactive Compounds ◽

Unsaturated Fatty Acids ◽

Animal Feed ◽

Lipid Fraction ◽

Industrial Applications ◽

Antioxidant Potential ◽

Total Phenolic ◽

Myrtus Communis ◽

Food Ingredient

The fatty acid (FA), polyphenol content and evaluation of the antioxidant capacity of exhausted Myrtus communis berries (EMB) resulting from the production of myrtle liqueur were assessed. All parts of the exhausted berries exhibited high concentrations of carbohydrates, proteins, lipids and phenolic compounds. The lipid fraction contained a high amount of poly unsaturated fatty acids (PUFA), mainly represented by linoleic acid (>70%). Of the phenolic acids evaluated by liquid chromatography/mass spectrometry, ellagic acid was the most predominant (>50%), followed by gallic and quinic acids. Quercetin and quercetin3-O-rhamnoside were the most abundant flavonoids. The seed extracts showed a higher antioxidant potential than the pericarp extracts; the same trend was observed for total phenolic compounds evaluated by spectrophotometric assay. The overall high content of bioactive compounds and the high antioxidant potential of this byproduct sustain its suitability for a number of industrial applications, such as a food ingredient in novel foods, an additive in cosmetic formulations or a component of animal feed formulations.

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Bioactive compounds and antioxidant potential of honey produced by africanized bees (Apis mellifera L.) in Piauí

Scientific Electronic Archives ◽

10.36560/13920201061 ◽

2020 ◽

Vol 13 (9) ◽

pp. 10

Author(s):

S. M. P. C. Silva ◽

W. C. Andrade ◽

P. C. Santos ◽

A. S. Nascimento ◽

C. A. L. Carvalho

Keyword(s):

Antioxidant Activity ◽

Apis Mellifera ◽

Phenolic Compounds ◽

Bioactive Compounds ◽

Reducing Power ◽

Antioxidant Potential ◽

Total Phenolic ◽

Botanical Origin ◽

Pollen Type ◽

Africanized Bees

This work evaluated the bioactive compounds and the antioxidant potential of honey produced by Africanized bees (Apis mellifera L.) in the state of Piauí, Brazil. The analyses show that all honeys investigatedpresented features of the pollen type Mimosa caesalpiniifolia. The content of phenolic compounds ranged from 27.65 to 97.01 mg in GAE g-1 of honey, with an average content of 62.66 ± 20.46 mgEAG g-1 of honey. The total flavonoid contents expressed in mg of quercentin/g of honey ranged from 5.43 to 13.02 mg of QE g-1, with an average of 8.33 ± 1.86 mg of QE g-1 of honey. The best performance of antioxidant activity by reducing power was EC50 of 0.15 and the lowest performance of antioxidant activity with IC50 was 1.24. The highest antioxidant activity (DPPH) ranged from 66.11 ± 0.02mg EAQ 100g-1, while the lowest activities were 9.16 and 10.88 mg EAQ 100g-1. The results show that the contents of total phenolic compounds, total flavonoids, DPPH antioxidant activity, and the pollen-reducing power from Piauí were influenced by botanical origin.

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Bioactive compounds from brewer’s spent grain: phenolic compounds, fatty acids and in vitro antioxidant capacity

Acta Scientiarum Technology ◽

10.4025/actascitechnol.v39i3.28435 ◽

2017 ◽

Vol 39 (3) ◽

pp. 269 ◽

Cited By ~ 3

Author(s):

Aline Da Rosa Almeida ◽

Márcia Regina Ferreira Geraldo ◽

Leomara Floriano Ribeiro ◽

Marcos Vieira Silva ◽

Matheus Vinicius De Oliveira Brisola Maciel ◽

...

Keyword(s):

Fatty Acids ◽

Antibacterial Activity ◽

Phenolic Compounds ◽

Polyunsaturated Fatty Acids ◽

Bioactive Compounds ◽

Antioxidant Potential ◽

Total Phenolic ◽

Brewer’S Spent Grain ◽

Spent Grain

Brewer's spent grain (BSG) was characterized by physicochemical, total phenolic compound and flavonoids contents. Antioxidant activity was evaluated by four different assays. The chromatographic analyses were used to quantify the phenolic compounds and the fatty acids in BSG. Ethanolic extracts were tested to evaluate antibacterial activity. The higher concentration of total phenolic compounds for BSG was obtained in the extraction with ethanol 20%. BSG showed an antioxidant potential for all tests evaluated. In the case of chromatographic analysis, phenolic acids and flavonoids, such as syringic acid and catechin, respectively, were detected in high quantities. Regarding to the fatty acids profile, polyunsaturated fatty acids, such as linoleic and oleic acids, were found in significant amounts. No antibacterial activity was reported for bacterial cultures and concentrations tested. BSG may be considered a protein source, rich in fiber, polyunsaturated fatty acids and bioactive compounds with antioxidant potential.

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Advances in the Chromatographic Separation and Determination of Bioactive Compounds for Assessing the Nutrient Profile of Nuts

Current Analytical Chemistry ◽

10.2174/1573411016999200729111951 ◽

2020 ◽

Vol 16 ◽

Author(s):

Natasa P. Kalogiouri ◽

Natalia Manousi ◽

Erwin Rosenberg ◽

George A. Zachariadis ◽

Victoria F. Samanidou

Keyword(s):

Fatty Acids ◽

Phenolic Compounds ◽

Bioactive Compounds ◽

High Performance ◽

Unsaturated Fatty Acids ◽

Nutritional Deficiencies ◽

Analytical Technique ◽

High Volatility ◽

Analytical Approaches

Background:: Nuts have been incorporated into guidelines for healthy eating since they contain considerable amounts of antioxidants and their effects are related to health benefits since they contribute to the prevention of nutritional deficiencies. The micronutrient characterization is based mainly on the determination of phenolics which is the most abundant class of bioactive compounds in nuts. Terpenes constitute another class of bioactive compounds that are present in nuts and show high volatility. The analysis of phenolic compounds and terpenes are very demanding tasks that require optimization of the chromatographic conditions to improve the separation of the components. Moreover, nuts are rich in unsaturated fatty acids and they are therefore considered as cardioprotective. Gas chromatography is the predominant instrumental analytical technique for the determination of derivatized fatty acids and terpenes in food matrices, while high performance liquid chromatography is currently the most popular technique for the determination of phenolic compounds Objective:: This review summarizes all the recent advances in the optimization of the chromatographic conditions for the determination of phenolic compounds, fatty acids and terpenes in nuts Conclusion:: The state-of-the art in the technology available is critically discussed, exploring new analytical approaches to reduce the time of analysis and improve the performance of the chromatographic systems in terms of precision, reproducibility, limits of detection and quantification and overall quality of the results

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High-Throughput Screening and Characterization of Phenolic Compounds in Stone Fruits Waste by LC-ESI-QTOF-MS/MS and Their Potential Antioxidant Activities

Antioxidants ◽

10.3390/antiox10020234 ◽

2021 ◽

Vol 10 (2) ◽

pp. 234 ◽

Cited By ~ 1

Author(s):

Yili Hong ◽

Zening Wang ◽

Colin J. Barrow ◽

Frank R. Dunshea ◽

Hafiz A. R. Suleria

Keyword(s):

Phenolic Compounds ◽

Antioxidant Capacity ◽

Prunus Persica ◽

Phenolic Content ◽

Antioxidant Potential ◽

Stone Fruit ◽

Total Phenolic ◽

Stone Fruits ◽

Antioxidant Power ◽

Pharmaceutical Industries

Stone fruits, including peach (Prunus persica L.), nectarine (Prunus nucipersica L.), plum (Prunus domestica L.) and apricot (Prunus armeniaca L.) are common commercial fruits in the market. However, a huge amount of stone fruits waste is produced throughout the food supply chain during picking, handling, processing, packaging, storage, transportation, retailing and final consumption. These stone fruits waste contain high phenolic content which are the main contributors to the antioxidant potential and associated health benefits. The antioxidant results showed that plum waste contained higher concentrations of total phenolic content (TPC) (0.94 ± 0.07 mg gallic acid equivalents (GAE)/g) and total flavonoid content (TFC) (0.34 ± 0.01 mg quercetin equivalents (QE)/g), while apricot waste contained a higher concentration of total tannin content (TTC) (0.19 ± 0.03 mg catechin equivalents (CE)/g) and DPPH activity (1.47 ± 0.12 mg ascorbic acid equivalents (AAE)/g). However, nectarine waste had higher antioxidant capacity in ferric reducing-antioxidant power (FRAP) (0.98 ± 0.02 mg AAE/g) and total antioxidant capacity (TAC) (0.91 ± 0.09 mg AAE/g) assays, while peach waste showed higher antioxidant capacity in 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay (0.43 ± 0.09 mg AAE/g) as compared to other stone fruits waste. Qualitative and quantitative phenolic analysis of Australian grown stone fruits waste were conducted by liquid chromatography coupled with electrospray-ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS/MS) and HPLC-photodiode array detection (PDA). The LC-ESI-QTOF-MS/MS result indicates that 59 phenolic compounds were tentatively characterized in peach (33 compounds), nectarine (28), plum (38) and apricot (23). The HPLC-PDA indicated that p-hydroxybenzoic acid (18.64 ± 1.30 mg/g) was detected to be the most dominant phenolic acid and quercetin (19.68 ± 1.38 mg/g) was the most significant flavonoid in stone fruits waste. Hence, it could be concluded that stone fruit waste contains various phenolic compounds and have antioxidant potential. The results could support the applications of these stone fruit wastes in other food, feed, nutraceutical and pharmaceutical industries.

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Microwave-Assisted Extraction and Ultrasound-Assisted Extraction of Bioactive Compounds from Grape Pomace

International Journal of Food Engineering ◽

10.1515/ijfe-2019-0191 ◽

2020 ◽

Vol 16 (1-2) ◽

Cited By ~ 2

Author(s):

Cassiano Brown da Rocha ◽

Caciano Pelayo Zapata Noreña

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Bioactive Compounds ◽

Grape Pomace ◽

Total Phenolic ◽

Microwave Assisted Extraction ◽

Ultrasound Assisted Extraction ◽

Microwave Assisted ◽

Assisted Extraction ◽

Ultrasound Assisted

AbstractThe grape pomace is a by-product from the industrial processing of grape juice, which can be used as a source of bioactive compounds. The aim of this study was to separate the phenolic compounds from grape pomace using an acidic aqueous solution with 2 % citric acid as a solvent, using both ultrasound-assisted extraction, with powers of 250, 350 and 450 W and times of 5, 10 and 15 min, and microwave-assisted extraction using powers of 600, 800 and 1,000 W and times of 5, 7 and 10 min. The results showed that for both methods of extraction, the contents of total phenolic compounds and antioxidant activity by ABTS and DPPH increased with time, and microwave at 1,000 W for 10 min corresponded to the best extraction condition. However, the contents of phenolic compounds and antioxidant activity were lower than exhaustive extraction using acidified methanol solution.

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Obtaining Bioactive Compounds from the Coffee Husk (Coffea arabica L.) Using Different Extraction Methods

Molecules ◽

10.3390/molecules26010046 ◽

2020 ◽

Vol 26 (1) ◽

pp. 46

Author(s):

Mariana de Oliveira Silva ◽

John Nonvignon Bossis Honfoga ◽

Lorena Lucena de Medeiros ◽

Marta Suely Madruga ◽

Taliana Kênia Alencar Bezerra

Keyword(s):

Phenolic Compounds ◽

Bioactive Compounds ◽

Extraction Methods ◽

Water Bath ◽

Raw Material ◽

Total Phenolic ◽

Total Phenolic Compounds ◽

Ethanol Mixture ◽

Coffee Husk ◽

Coffea Arabica L

Coffee husks (Coffea arabica L.) are characterized by exhibiting secondary metabolites such as phenolic compounds, which can be used as raw material for obtaining bioactive compounds of interest in food. The objective of this study is to evaluate different methods for obtaining the raw material and extracting solutions of bioactive compounds from coffee husks. Water bath and ultrasound-assisted extraction methods were used, using water (100%) or ethanol (100%) or a mixture of both (1:1) as extracting solutions and the form of the raw material was in natura and dehydrated. The extracts were evaluated by their antioxidant potential using DPPH radicals, ABTS, and iron reduction (ferric reducing antioxidant power (FRAP)), and later total phenolic compounds, total flavonoids, and condensed tannins were quantified the phenolic majority compounds were identified. It was verified that the mixture of water and ethanol (1:1) showed better extraction capacity of the compounds with antioxidant activity and that both conventional (water bath) or unconventional (ultrasound) methods showed satisfactory results. Finally, a satisfactory amount of bioactive compounds was observed in evaluating the chemical composition (total phenolic compounds, total flavonoids, condensed tannins, as well as the analysis of the phenolic profile) of these extracts. Corroborating with the results of the antioxidant activities, the best extracting solution was generally the water and ethanol mixture (1:1) using a dehydrated husk and water bath as the best method, presenting higher levels of the bioactive compounds in question, with an emphasis on chlorogenic acid. Thus, it can be concluded that the use of coffee husk as raw material to obtain extracts of bioactive compounds is promising. Last, the conventional method (water bath) and the water and ethanol mixture (1:1) stood out among the methods and extracting solutions used for the dehydrated coffee husk.

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Antioxidant activities and A549 lung adenocarcinomic cells against capacity from various extracts of Agrimonia pilosa Ledeb

Journal of Ayurvedic and Herbal Medicine ◽

10.31254/jahm.2018.4304 ◽

2018 ◽

Vol 4 (3) ◽

pp. 114-119

Author(s):

Quang Ung Le ◽

◽

Horng Liang Lay ◽

Ming Chang Wu ◽

◽

...

Keyword(s):

Phenolic Compounds ◽

High Performance ◽

Antioxidant Activities ◽

Radical Scavenging ◽

A549 Cells ◽

Total Phenolic ◽

Root Extract ◽

Inhibitory Capacity ◽

Ethanol Extracts

Agrimonia pilosa Ledeb (AL) has received considerable attention as a herbal medicine for its applications in ethnopharmacology with heath benefits. This study aimed to investigate antioxidant activities and A549 growth inhibitory capacity from its root extract (RE) and aerial parts extract (AE). The 50% ethanol extracts were used for the tests. The total polyphenol content and the antioxidant effects comprising ABTS+ and DPPH free radical scavenging activities were evaluated. Phenolic compounds in the extracts were isolated using high performance liquid chromatography (HPLC). Lactate dehydrogenase released in medium was also evaluated. Total phenolic and flavonoid content, and in vitro antioxidant potential of the RE were higher (p<0.01) than that of the AE. Two individual phenolic compounds consisting of 4-hydroxybenzoic acid and p-coumaric acid were firstly identified in both by HPLC. The RE exhibited higher A549 inhibitory capacity compared to the AE and activated the apoptotic proteins of bcl-2, bax, bad, caspase-3 and caspase-9 in A549. In conclusion, the AL extracts were more effective in antioxidant and A549 cells inhibitory capacity.

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First application of different Chlorella sp. microalgal strains for the treatment of vegetation waters derived from unconventional oil extractions enriched with citrus byproducts

10.20944/preprints202106.0616.v1 ◽

2021 ◽

Author(s):

Carolina Chiellini ◽

Monica Macaluso ◽

Adriana Ciurli ◽

Lorenzo Guglielminetti ◽

Isabella Taglieri ◽

...

Keyword(s):

Phenolic Compounds ◽

Olive Oil ◽

Low Cost ◽

Environmental Pollutants ◽

Extraction Process ◽

Total Phenolic ◽

Mediterranean Countries ◽

Extraction Processes ◽

The Mediterranean ◽

Vegetation Water

The Mediterranean diet has among its cornerstones the use of olive oil for its nutraceutical and organoleptic properties. Despite the numerous merits, olive-oil mill wastewater (OMWW), which is generated by the olive-oil extraction process, is one of the most serious environmental pollutants in the Mediterranean countries. The polluting potential of OMWW is due to its high content of tannins, polyphenols, polyalcohols, pectins and lipids. In this experiment, we tested the ability of five microalgae of the Chlorella group (SEC_LI_ChL_1, CL-Sc, CL-Ch, FB and Idr) in lowering the percentage of total phenolic compounds in vegetation water. In order to close the recovery cycle of a fortified citrus olive oils previously developed, we tested the vegetation wa-ter obtained with three different extraction processes (conventional, lemon and orange peels) at three concentrations each (10%, 25% and 50%). Results showed that strains Idr, FB and CL-Sc from the Lake Massaciuccoli can tolerate vegetation water from conventional and lemon peels extraction up to 25%; these strains can also reduce the phenolic compounds within the tests. The results demonstrate that the application of microalgae for OMWW treatment represent an inter-esting opportunity, and an eco-friendly low-cost solution to be developed within the companies as a full-scale approach.

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