scholarly journals A Box-Behnken Design for Optimal Green Extraction of Compounds from Olive Leaves That Potentially Activate the AMPK Pathway

2020 ◽  
Vol 10 (13) ◽  
pp. 4620
Author(s):  
Beatriz Martín-García ◽  
Sandra Pimentel-Moral ◽  
Ana María Gómez-Caravaca ◽  
David Arráez-Román ◽  
Antonio Segura-Carretero
Keyword(s):  
High Performance ◽  
Optimum Conditions ◽  
Olive Leaves ◽  
Box Behnken Design ◽  
Green Extraction ◽  
Flight Mass Spectrometry ◽  
Assisted Extraction ◽  
Intracellular Lipid Accumulation ◽  
Treatment Of Obesity ◽  
Amp Activated Protein Kinase

Olive leaves contain bioactive compounds that have been shown to activate AMP-activated protein kinase (AMPK), which decreases intracellular lipid accumulation. Microwave-assisted extraction (MAE) is a green extraction technique that is frequently used in the recovery of phenolic compounds from plants. Thus, in this study, a Box-Behnken design was used to optimize MAE conditions such as temperature, percentage of ethanol and extraction time to obtain the maximum content of total compounds and compounds that activate AMPK. To this end, all extracts were characterized by High-Performance Liquid Chromatography Coupled to Electrospray Ionization Time-of-Flight Mass Spectrometry (HPLC-ESI-TOF-MS). The optimum conditions to obtain the highest content of total compounds were 123 °C, 100% of ethanol/water (v/v) and 23 min, whereas the optimum conditions for the highest amount of compounds that activate AMPK were 111 °C, 42% of ethanol/water (v/v) and 23 min. Thus, a multi-analysis by desirability was carried out to establish MAE optimal conditions for both responses. The optimum conditions were 111 °C, 100% EtOH and 23 min with a desirability of 0.97, which means that the responses are close to their individual optimal values. As a result, the olive leaf extract obtained at these optimal MAE conditions has great potential to be effective in the treatment of obesity.

scholarly journals Sweet Cherry Byproducts Processed by Green Extraction Techniques as a Source of Bioactive Compounds with Antiaging Properties

Antioxidants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 418 ◽  
Author(s):  
Luz Agulló-Chazarra ◽  
Isabel Borrás-Linares ◽  
Jesús Lozano-Sánchez ◽  
Antonio Segura-Carretero ◽  
Vicente Micol ◽  
...  
Keyword(s):  
Supercritical Co2 ◽  
High Performance ◽  
Food Industry ◽  
Sweet Cherry ◽  
Subcritical Water ◽  
Extraction Techniques ◽  
Green Extraction ◽  
Flight Mass Spectrometry ◽  
Cosmetic Industry ◽  
Continuous Demand

In the cosmetic industry, there is a continuous demand for new and innovative ingredients for product development. In the context of continual renovation, both cosmetic companies and customers are particularly interested in compounds derived from natural sources due to their multiple benefits. In this study, novel and green-extractive techniques (pressurized solvent, supercritical CO2, and subcritical water extractions) were used to obtain three new extracts from sweet cherry stems, a byproduct generated by the food industry. The extracts were characterized by high-performance liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS), and 57 compounds, mainly flavonoids but also organic and phenolic acids, fatty acids, and terpenes, were identified. After analytical characterization, a multistep screening approach, including antioxidant, enzymatic, and photoprotective cellular studies, was used to select the best extract according to its benefits of interest to the cosmetics industry. The extract obtained with supercritical CO2 presented the best characteristics, including a wide antioxidant capacity, especially against lipid peroxyl and •OH free radicals, as well as relevant photoprotective action and antiaging properties, making it a potential new ingredient for consideration in the development of new cosmetics.

scholarly journals Optimization of process variables for phyllanthin extraction from Phyllanthus amarus leaves by supercritical fluid using a Box-Behnken experimental design followed by HPLC identification

2013 ◽  
Vol 63 (2) ◽  
pp. 193-207 ◽  
Author(s):  
Ajit A. Patil ◽  
Sachin S. Bhusari ◽  
Devanand B. Shinde ◽  
Pravin S. Wakte
Keyword(s):  
Carbon Dioxide ◽  
Hplc Analysis ◽  
Extraction Time ◽  
Phyllanthus Amarus ◽  
Process Variables ◽  
Optimum Conditions ◽  
Box Behnken Design ◽  
Assisted Extraction ◽  
Predicted Values ◽  
Good Agreement

The response surface methodology using the Box-Behnken design was established to describe supercritical carbon dioxide assisted extraction of phyllanthin from Phyllanthus amarus Schum and Thonn leaves prior to HPLC analysis. The effects of extraction pressure, temperature, modifier concentration and extraction time on the yield of phyllanthin were investigated. By solving the regression equation, the optimum conditions were as follows: extraction pressure 23.2 MPa, temperature 40 °C, methanol as modifier at a concentration of 10 % and time 90 min. Under these conditions, the phyllanthin yield was 12.83 ± 0.28 mg g-1, which was in good agreement with the predicted values. Modifier concentration and extraction time showed a significant effect on the phyllanthin yield.

scholarly journals Intensification of Polyphenol Extraction from Olive Leaves Using Ired-Irrad®, an Environmentally-Friendly Innovative Technology

Antioxidants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 227 ◽  
Author(s):  
Abi-Khattar ◽  
Rajha ◽  
Abdel-Massih ◽  
Maroun ◽  
Louka ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Inhibitory Concentration ◽  
Operating Conditions ◽  
Innovative Technology ◽  
Total Phenolic ◽  
Olive Leaves ◽  
Assisted Extraction ◽  
Similar Quantity

Optimization of infrared-assisted extraction was conducted using Response Surface Methodology (RSM) in order to intensify polyphenol recovery from olive leaves. The extraction efficiency using Ired-Irrad®, a newly-patented infrared apparatus (IR), was compared to water bath (WB) conventional extraction. Under optimal conditions, as suggested by the model and confirmed experimentally, the total phenolic content yield was enhanced by more than 30% using IR as contrasted to WB, which even required 27% more ethanol consumption. High Performance Liquid Chromatography analyses quantified the two major phenolic compounds of the leaves: Oleuropein and hydroxytyrosol, which were both intensified by 18% and 21%, respectively. IR extracts increased the antiradical activity by 25% and the antioxidant capacity by 51% compared to WB extracts. On the other hand, extracts of olive leaves obtained by both techniques exhibited equal effects regarding the inhibition of 20 strains of Staphylococcus aureus, with a minimum inhibitory concentration (MIC) varying between 3.125 and 12.5 mg/mL. Similarly, both extracts inhibited Aflatoxin B1 (AFB1) secretion by Aspergillus flavus, with no growth inhibition of the fungus. Finally, optimization using RSM allowed us to suggest other IR operating conditions aiming at significantly reducing the consumption of energy and solvent, while maintaining similar quantity and quality of phenolic compounds as what is optimally obtained using WB.

scholarly journals Extraction Optimization of 5,7-Dihydroxy-6,8,4′-trimethoxyflavonol, a Bioactive Flavonoid from Rubus rosifolius (Rosaceae) Leaves

2019 ◽  
Vol 14 (1) ◽  
pp. 1934578X1901400
Author(s):  
Janieire Lorraine da Rocha Pittarello ◽  
Marcel Petreanu ◽  
Valdir Cechinel Filho ◽  
Clóvis Antonio Rodrigues ◽  
Luiz Carlos Klein-Júnior ◽  
...  
Keyword(s):  
Extraction Method ◽  
High Performance ◽  
Solvent Ratio ◽  
Flavonoid Content ◽  
Box Behnken Design ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction ◽  
Addition Level

Recently, it has been demonstrated that a rare flavonoid, 5,7-dihydroxy-6,8,4′-trimethoxyflavonol (1), isolated from the leaves of Rubus rosifolius showed significant antiproliferative activity against different cancer cell lines and had a diuretic effect. This study aimed to determine the best extraction method evaluating dynamic maceration extraction (DME), ultrasonic assisted extraction (UAE), and microwave assisted extraction (MAE). In addition, level optimization for different factors was focused using Design of Experiments. The flavonoid content was estimated by high performance liquid chromatography (HPLC). The results indicated that UAE provided the highest content with the shortest extraction time. For optimization, response surface methodology (RSM) was used, applying a Box-Behnken Design (BBD) with three factors at three levels. It was possible to observe that the flavonoid content significantly increased with the increase of temperature (max. 70°C). The time had little influence on the extraction and, for this reason, was set at 25 min. With regard to the drug/solvent ratio, it was observed that this had an impact on yield with a ratio of 3.5%. A model was built and a determination coefficient of 0.98 was obtained. After validation, an optimized method was developed, able to extract 1 with a lower time, lower solvent consumption, and affording higher contents.

scholarly journals Optimization and Comparison of Ultrasound and Microwave-Assisted Extraction of Phenolic Compounds from Cotton-Lavender (Santolina chamaecyparissus L.)

Agronomy ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 84
Author(s):  
Mohammed Aourach ◽  
Ana V. González-de-Peredo ◽  
Mercedes Vázquez-Espinosa ◽  
Haiat Essalmani ◽  
Miguel Palma ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Chlorogenic Acid ◽  
High Performance ◽  
Antimicrobial Agents ◽  
Flight Mass Spectrometry ◽  
Assisted Extraction ◽  
Efficient Extraction ◽  
Natural Phenolic Compounds ◽  
First Time ◽  
Sample Ratio

The interest in natural phenolic compounds has increased because of their attractive use especially as antioxidant and antimicrobial agents in foods. The large content in phenolic compounds of interest in Santolina chamaecyparissus L. (S. chamaecyparissus) makes this plant a target source that is worthy of note. In this work, new extraction technologies comprising ultrasound (UAE) and microwave (MAE) assisted extraction of the phenolic compounds in S. chamaecyparissus have been developed, optimized, and compared. Several extraction factors have been optimized based on a Box-Behnken design. Such optimized factors include the percentage of methanol in water (25–75%), the temperature (10–70 °C), the ultrasound amplitude (20–80%), the ultrasound cycle (0.2–1 s), the solvent pH (2–7) and the solvent-sample ratio (5/0.2–15/0.2 mL/g) with regard to UAE, while the percentage of methanol in water (50–100%), the temperature (50–100 °C), the pH (2–7) and the solvent-sample ratio (5/0.2–15/0.2 mL/g) were optimized for MAE. The solvent composition was the most influential parameter both on MAEs (64%) and UAEs (74%). The extraction optimum time was established as 15 min for MAE and 25 min for UAE. Five major phenolic compounds were detected and identified by Ultra-High-Performance Liquid Chromatography—Quadrupole Time of Flight—Mass Spectrometry (UHPLC-QToF-MS) in the extracts: chlorogenic acid, quercetin 3-O-galactoside, quercetin 3-O-glucoside, isoorientin, and cynarin. With the exception of chlorogenic acid, the other four compounds have been identified for the first time in S. chamaecyparissus. The findings have confirmed that MAE is a significantly more efficient extraction method than UAE to extract phenolic compounds from S. chamaecyparissus.

scholarly journals Pengayaan Polifenol Total dari Daun Kadamba Menggunakan Metode Ekstraksi Berbantu Mikrowave Berbasis Pelarut Hijau

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Islamudin Ahmad ◽  
Andi Yusniah ◽  
Yuspian Nur ◽  
Wisnu Cahyo Prabowo ◽  
Herman
Keyword(s):  
Malic Acid ◽  
Deep Eutectic Solvent ◽  
Green Solvent ◽  
Optimum Conditions ◽  
Total Polyphenols ◽  
Box Behnken Design ◽  
Mitragyna Speciosa ◽  
Assisted Extraction ◽  
Natural Deep Eutectic Solvent ◽  
Optimum Extraction

The present study aims to optimize the natural deep eutectic solvent (NADES) as a green solvent-based microwave-assisted extraction (MAE) of total polyphenols content (TPC) from Mitragyna speciosa Korth Havil leaves using response surface methodology (RSM). Preparation of natural deep eutectic solvent (NADES) as a green solvent was performed by melting the two-component of malic acid and glucose using a magnetic stirrer. The leaves sample was extracted using the NADES-MAE method with various extraction conditions with four factors and three levels (Box Behnken Design) and optimized using RSM with licensed software of Design Expert V12. TPC was determined using a Folin-Ciocalteau reagent and absorbance was measured on a UV-VIS spectrophotometer at 770 nm and gallic acid as a standard. This study was obtained the optimum extraction conditions include: NADES ratio (malic acid: glucose) of 1:2 g/g, 50% microwave power, a solvent-sampel ratio of 12:1 mL/g, and extraction time for 7 minutes. The optimum conditions were obtained according to the equation formula: TPC = 165.17 – 33.97X1 + 38.36X2 – 6.08X3 + 16.12X4 – 62.77X1X2 + 79.61X12X2, where a R2 value = 0.7102 with a TPC prediction of 402.087±43.81 mg GAE/g sample. These conditions can be used to extract TPC from this plant efficiently, quickly, easily, and environmentally friendly.

Extraction Optimization of Antibacterial Substances from Amomum tsao-ko

2012 ◽  
Vol 430-432 ◽  
pp. 1036-1039 ◽  
Author(s):  
Wen Xue Chen ◽  
Mei Fang Peng ◽  
Yue Ying Hu ◽  
Hou Yuan Qiu
Keyword(s):  
Extraction Procedure ◽  
The Other ◽  
Optimum Conditions ◽  
Antibacterial Compounds ◽  
Soaking Time ◽  
Box Behnken Design ◽  
Ethanol Extraction ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

The aim of this work was to optimize extraction procedure of antibacterial compounds from amomum tsao-ko and verify antibacterial ability of yields. Two methods were carried out and compared. One is ethanol reflux extraction, and the other is ultrasonic-assisted ethanol extraction which selected due to better antibacterial efficiency of the extract obtained. In order to improve extraction rate and antibacterial activity which both varied with ultrasonic-assisted extraction conditions, Box-Behnken design was employed. Studies showed that the optimum conditions for extraction antibacterial substances from amomum tsao-ko were determined to be: ethanol density 100%, soaking time 4h, extraction time 120min.

scholarly journals Box–Behnken Design (BBD)-Based Optimization of Microwave-Assisted Extraction of Parthenolide from the Stems of Tarconanthus camphoratus and Cytotoxic Analysis

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 1876
Author(s):  
Perwez Alam ◽  
Nasir Ali Siddiqui ◽  
Md. Tabish Rehman ◽  
Afzal Hussain ◽  
Ali Akhtar ◽  
...  
Keyword(s):  
High Performance ◽  
Signal To Noise Ratio ◽  
Suitable Model ◽  
Extraction Temperature ◽  
Microwave Assisted Extraction ◽  
Box Behnken Design ◽  
Microwave Assisted ◽  
Cytotoxic Compound ◽  
Assisted Extraction ◽  
Efficient Extraction

Parthenolide, a strong cytotoxic compound found in different parts of Tarchonanthus camphoratus which motivated the authors to develop an optimized microwave-assisted extraction (MEA) method using Box–Behnken design (BBD) for efficient extraction of parthenolide from the stem of T. camphoratus and its validation by high-performance thin-layer chromatography (HPTLC) and cytotoxic analysis. The optimized parameters for microwave extraction were determined as: 51.5 °C extraction temperature, 50.8 min extraction time, and 211 W microwave power. A quadratic polynomial model was found the most suitable model with R2 of 0.9989 and coefficient of variation (CV) of 0.2898%. The high values of adjusted R2 (0.9974), predicted R2 (0.9945), and signal-to-noise ratio (74.23) indicated a good correlation and adequate signal, respectively. HPTLC analyzed the parthenolide (Rf = 0.16) content in T. camphoratus methanol extract (TCME) at λmax = 575 nm and found it as 0.9273% ± 0.0487% w/w, which was a higher than expected yield (0.9157% w/w). The TCME exhibited good cytotoxicity against HepG2 and MCF-7 cell lines (IC50 = 30.87 and 35.41 µg/mL, respectively), which further supported our findings of high parthenolide content in TCME. This optimized MAE method can be further applied to efficiently extract parthenolide from marketed herbal supplements containing different Tarconanthus species.

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