scholarly journals Exercise Training of Secreted Protein Acidic and Rich in Cysteine (Sparc) KO Mice Suggests That Exercise-Induced Muscle Phenotype Changes Are SPARC-Dependent

2020 ◽  
Vol 10 (24) ◽  
pp. 9108 ◽  
Author(s):  
Abdelaziz Ghanemi ◽  
Aicha Melouane ◽  
Mayumi Yoshioka ◽  
Jonny St-Amand
Keyword(s):  
Body Weight ◽  
Tibialis Anterior Muscle ◽  
The Body ◽  
Type I ◽  
Secreted Protein ◽  
Adaptation Period ◽  
Weight Percentage ◽  
Lactate Levels ◽  
Exercise Induced ◽  
Induced Changes

We previously identified secreted protein acidic and rich in cysteine (Sparc) as an exercise-induced gene in young and elderly individuals. Via this animal experiment, we aim to identify selected implications of SPARC mainly within the muscle in the contexts of exercise. Mice were divided into eight groups based on three variables (age, genotype and exercise): Old (O) or young (Y) × Sparc knock-out (KO) or wild-type (WT) × sedentary (Sed) or exercise (Ex). The exercised groups were trained for 12 weeks at the lactate threshold (LT) speed (including 4 weeks of adaptation period) and all mice were sacrificed afterwards. Body and selected tissues were weighed, and lactate levels in different conditions measured. Expression of skeletal muscle (SM) collagen type I alpha 1 chain (COL1A1) and mitochondrially encoded cytochrome c oxidase I (MT-CO1) in addition to SM strength (grip power) were also measured. Ageing increased the body and white adipose tissue (WAT) weights but decreased SM weight percentage (to body weight) and MT-CO1 expression (in WT). Exercise increased SM COL1A1 in WT mice and MT-CO1 expression, as well as weight percentage of the tibialis anterior muscle, and decreased WAT weight (trend). Compared to WT mice, Sparc KO mice had lower body, muscle and WAT weights, with a decrease in SM MT-CO1 and COL1A1 expression with no genotype effect on lactate levels in all our blood lactate measures. Sparc KO effects on body composition, adiposity and metabolic patterns are toward a reduced WAT and body weight, but with a negative metabolic and functional phenotype of SM. Whereas such negative effects on SM are worsened with ageing, they are relatively improved by exercise. Importantly, our data suggest that the exercise-induced changes in the SM phenotype, in terms of increased performance (metabolic, strength and development), including lactate-induced changes, are SPARC-dependent.

Structure and function of the ankle dorsiflexor muscles in young and moderately active men and women

2003 ◽  
Vol 95 (6) ◽  
pp. 2416-2424 ◽  
Author(s):  
Anna Maria Holmbäck ◽  
Michelle M. Porter ◽  
David Downham ◽  
Jesper L. Andersen ◽  
Jan Lexell
Keyword(s):  
Body Weight ◽  
Tibialis Anterior Muscle ◽  
The Body ◽  
Type I ◽  
Cross Sectional ◽  
Men And Women ◽  
Specific Strength ◽  
Multiple Biopsies ◽  
And Function ◽  
Ankle Dorsiflexor

The aim was to investigate determinants of ankle dorsiflexor muscle (DF) strength and size in moderately active young men and women ( n = 30; age 20–31 yr). Concentric (Con) and eccentric (Ecc) strength were measured isokinetically. Magnetic resonance imaging was used to determine the muscle cross-sectional area (CSA). Multiple biopsies were obtained from the tibialis anterior muscle to determine total numbers, areas (Area I and II) and proportions (Prop I and II) of type I and II fibers, respectively, and relative contents of myosin heavy chain (MHC) isoforms MHC1, MHC2a, and MHC2x. Women had lower Con and Ecc strength (24 and 27%; P < 0.01), smaller CSA (19%; P < 0.001), lower Ecc DF specific strength (strength/CSA) (10%; P < 0.01), and smaller Area I and Area II (21 and 31%; P < 0.01) than men. Prop I, MHC1, estimated total number of fibers, and Con DF specific strength were similar for both sexes. Con DF strength was up to 72% determined by CSA and Prop I, and Ecc DF strength was up to 81% determined by CSA, Prop I, and sex; variables other than CSA explained at most 9%. Body weight and fiber areas explained >50% of the variation in CSA. In conclusion, CSA was the predominant determinant of DF strength, CSA was to a great extent determined by the body weight and the sizes of muscle fibers, and sex differences in Ecc specific strength require further study.

WING MOVEMENTS AND LIFT REGULATION IN THE FLIGHT OF DESERT LOCUSTS

1993 ◽  
Vol 182 (1) ◽  
pp. 57-69 ◽  
Author(s):  
M. Wortmann ◽  
W. Zarnack
Keyword(s):  
Body Weight ◽  
Unsteady Aerodynamics ◽  
Movement Patterns ◽  
The Body ◽  
Three Dimensions ◽  
Body Angle ◽  
Wing Movement ◽  
Induced Changes ◽  
Lift Body

1. We simultaneously recorded lift/body weight, flight speed, body angle and 12 variables of wing movement for locusts performing tethered long-term flight with low movement scatter. The movements of the forewings and hindwings were recorded in three dimensions by means of miniature induction coils. 2. By adjusting the body angle, we could reproducibly manipulate lift generation as a consequence of induced changes in the wings' movement patterns. We were therefore able to analyse various relationships between the movement patterns and lift. 3. The most prominent variations of kinematic variables were observed for the forewing movements. The relative lift and the steady angle of pitch were positively correlated but there was a negative correlation between relative lift and pitching amplitude. We found no correlation between relative lift and flapping amplitude. Our results seem to correspond to a new theory about unsteady aerodynamics of oscillating aerofoils. 4. We sometimes observed variations in lagging. 5. The forewing downstroke was delayed by 0–8 ms following the hindwing downstroke. Relative lift was positively correlated to this delay.

Dorsomedial hypothalamic corticotropin-releasing factor mediation of exercise-induced anorexia

2005 ◽  
Vol 288 (6) ◽  
pp. R1800-R1805 ◽  
Author(s):  
Maiko Kawaguchi ◽  
Karen A. Scott ◽  
Timothy H. Moran ◽  
Sheng Bi
Keyword(s):  
Gene Expression ◽  
Body Weight ◽  
Food Intake ◽  
Mrna Expression ◽  
Critical Role ◽  
Voluntary Exercise ◽  
Running Wheel ◽  
Exercise Induced ◽  
Running Wheels ◽  
Induced Changes

Running wheel access and resulting voluntary exercise alter food intake and reduce body weight. The neural mechanisms underlying these effects are unclear. In this study, we first assessed the effects of 7 days of running wheel access on food intake, body weight, and hypothalamic gene expression. We demonstrate that running wheel access significantly decreases food intake and body weight and results in a significant elevation of CRF mRNA expression in the dorsomedial hypothalamus (DMH) but not the paraventricular nucleus. Seven-day running wheel access also results in elevated arcuate nucleus and DMH neuropeptide Y gene expression. To assess a potential role for elevated DMH CRF activity in the activity-induced changes in food intake and body weight, we compared changes in food intake, body weight, and hypothalamic gene expression in rats receiving intracerebroventricular (ICV) CRF antagonist α-helical CRF or vehicle with or without access to running wheels. During a 4-day period of running wheel access, we found that exercise-induced reductions of food intake and body weight were significantly attenuated by ICV injection of the CRF antagonist. The effect on food intake was specific to a blockade of activity-induced changes in meal size. Central CRF antagonist injection further increased DMH CRF mRNA expression in exercised rats. Together, these data suggest that DMH CRF play a critical role in the anorexia resulting from increased voluntary exercise.

scholarly journals Latihan yoga terhadap berat badan, persen lemak tubuh, dan lingkar perut pada wanita dewasa overweight

2018 ◽  
Vol 14 (4) ◽  
pp. 131
Author(s):  
Putri Nur Fatimah ◽  
Fillah Fithra Dieny ◽  
Etisa Adi Murbawani ◽  
Ahmed Fahmy Arif Tsani
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Waist Circumference ◽  
Nutrition Education ◽  
Body Fat ◽  
The Body ◽  
Control Group ◽  
Weight Percentage ◽  
Percentage Of Body Fat ◽  
Significant Difference

Background: Obesity caused diabetes and cardiovascular disease. Yoga considered three aspects, physic, emotion and mental that helped in obesity therapy.Objective: This research aimed to explain the influence of yoga excercise on body weight, percent of body fat, waist circumference and waist to hip ratio (WHR) in overweight women.Method: This research applied quasi experiment design by using pre-post test with control group design. The subjects of research were 31 women aged 19-25 years old and divided into 2 groups, treatment group and control group. Treatment group was prepared to do yoga in 60 minutes for 10 times during 20 days and given nutrition education, whereas control group was given nutrition education only. Data body weight and percentage of body fat were measured by bioimpedance analysis; waist circumference and WHR were measured by tape measures; food intake were assessed by food recall and food frequency questionnaire. Data were analyzed using paired t-test and independent t-test.Results: There was a significant difference of body weight before and after intervention in both groups. The body weight on treatment group decreased by 0.81 ± 1.29 kg (p<0.05), while the body weight on the control group increased. Percentage of body fat and waist circumference decreased on both of groups, however treatment group decreased more than the control group. WHR no significant on both of groups. Yoga excercise resulted no significant influences on body weight, percentage of body fat, waist circumference and WHR in overweight women (p>0.05). Conclusion: Obesity women with 10 times yoga had no significant influences on body weight, percentage of body fat, waist circumference and WHR.

scholarly journals Energy and protein requirements of crossbred cattle in feedlot

2016 ◽  
Vol 37 (2) ◽  
pp. 1029 ◽  
Author(s):  
Maria Luciana Menezes Wanderley Neves ◽  
Antonia Sherlânea Chaves Véras ◽  
Evaristo Jorge Oliveira de Souza ◽  
Marcelo De Andrade Ferreira ◽  
Sebastião De Campos Valadares Filho ◽  
...  
Keyword(s):  
Body Weight ◽  
Reference Group ◽  
The Body ◽  
Metabolizable Energy ◽  
Net Energy ◽  
Adaptation Period ◽  
Protein Requirements ◽  
Randomized Design ◽  
Dairy Bulls ◽  
Slaughter Method

The objective of this study is to predict the energy and protein requirements of crossbred dairy cattle in feedlot. The study was conducted at the Unidade Acadêmica de Serra Talhada, Universidade Federal Rural de Pernambuco, Brazil with 30 bulls with a body weight of 339.1 ± 35.4 kg. Five animals were slaughtered at the end of the adaptation period to serve as the reference group; the remainder of the animals was slaughtered after 112 days. The latter group was randomly allocated to receive five treatments: 0, 17, 34, 51 and 68% of concentrate in the feed using a completely randomized design. The dietary intake of the animals that were not given concentrate was restricted to 1.5% of their body weight; these animals composed the group fed for maintenance. The body composition and empty body weight (EBW) were estimated by means of the comparative slaughter method and full dissection of a half-carcass. The results showed that for crossbred dairy bulls in confinement, the net and metabolizable energy requirements were 86.49 and 138 kcal EBW-0.75 day-1, respectively, and the efficiency of use of metabolizable energy for maintenance and gain were 62.67% and 31.67%, respectively. The net energy (NEg) and net protein (NPg) requirements for gain can be estimated using the following equations, respectively: NEg= 0.0392*EBW0.75*EBWG1.0529 and NPg= 242.34 x EBWG - 23.09 x RE. The efficiency of use of metabolizable protein for gain was 25.8%, and the protein requirement for maintenance was 2.96 g EBW-0.75 day-1. The rumen degradable protein can supply 62.44% of the crude protein requirements of feedlot dairy crossbred bulls with a body weight of 450 kg while gaining 1 kg day-1.

scholarly journals The Effects of Dehydration on Metabolic and Neuromuscular Functionality during Cycling

2020 ◽  
Vol 17 (4) ◽  
pp. 1161 ◽  
Author(s):  
Francesco Campa ◽  
Alessandro Piras ◽  
Milena Raffi ◽  
Aurelio Trofè ◽  
Monica Perazzolo ◽  
...  
Keyword(s):  
Body Weight ◽  
Muscle Activation ◽  
Minute Ventilation ◽  
Bioelectrical Impedance ◽  
Biceps Femoris ◽  
Major Axis ◽  
The Body ◽  
Fluid Loss ◽  
Significant Difference ◽  
Exercise Induced

This study aimed to determine the effects of dehydration on metabolic and neuromuscular functionality performance during a cycling exercise. Ten male subjects (age 23.4 ± 2.7 years; body weight 74.6 ± 10.4 kg; height 177.3 ± 4.6 cm) cycled at 65% VO2max for 60 min followed by a time-to-trial (TT) at 95% VO2max, in two different conditions: dehydration (DEH) and hydration (HYD). The bioelectrical impedance vector analysis (BIVA) and body weight measurements were performed to assess body fluid changes. Heart rate (HR), energy cost, minute ventilation, oxygen uptake, and metabolic power were evaluated during the experiments. In addition, neuromuscular activity of the vastus medialis and biceps femoris muscles were assessed by surface electromyography. After exercise induced dehydration, the bioimpedance vector significantly lengthens along the major axis of the BIVA graph, in conformity with the body weight change (−2%), that indicates a fluid loss. Metabolic and neuromuscular parameters significantly increased during TT at 95% VO2max with respect to constant workload at 65% of VO2max. Dehydration during a one-hour cycling test and subsequent TT caused a significant increase in HR, while neuromuscular function showed a lower muscle activation in dehydration conditions on both constant workload and on TT. Furthermore, a significant difference between HYD and DEH for TT duration was found.

scholarly journals Effect of Ocimum sanctum Linn (Tulsi) on body weight and some biochemical parameters in restraint stressed albino rats

1970 ◽  
Vol 3 ◽  
pp. 29-34
Author(s):  
Dilruba Siraji ◽  
Nadira Islam ◽  
Noorzahan Begum ◽  
Sultana Ferdousi
Keyword(s):  
Body Weight ◽  
Biochemical Parameters ◽  
Restraint Stress ◽  
Biochemical Parameter ◽  
The Body ◽  
Ocimum Sanctum ◽  
Control Group ◽  
Albino Rats ◽  
Stressed Group ◽  
Induced Changes

Background: With the increased trend of urbanization of our society, the incidence of various stress related diseases are increasing day by day. People under chronic stress often tends to seek relief through drugs, which may have side effects. But some natural supplements called adaptogen, can be more beneficial in reducing symptoms of stress. Objective: The present animal model experimental study was designed to observe the effects of restraint stress on body weight, serum ALT, AST, Glucose, Cholesterol and their modifications by Ocimum sanctum Linn (tulsi) pretreatment which is an Ayurvedic adaptogen and has a long history of therapeutic use. Study design: For this purpose, 30 albino rats aged 90 to 120 days were included in this study. Twenty rats of experimental group were further subdivided into two groups. One group consisted of 10 rats exposed to one hour restraint stress daily for 7 days and 10 rats of the second group were pretreated with tulsi for 7 days before exposure to stress in the same way. 10 non stressed , non pretreated rats were taken into control group.This study was undertaken in the laboratory of the Department of Physiology,BSMMU during the period from July 2003 to June 2004. Methods: The body weight of all control rats were recorded daily in the morning. Body weight of the experimental rats were recorded just before exposure to stress. After completing the experiment , the animals were sacrificed and blood was collected . Serum glucose, cholesterol and ALT,ASTwere determined by standard laboratory technique. Data were compared among the groups and the results were statistically analyzed using unpaired student t' test. Results : The body weight in untreated stressed group was significantly lower (p < 0.001) than those of the control group and tulsi pretreated group. Serum levels of glucose, cholesterol, aminotrasferases (ALT and AST) were significantly higher (p < 0.001) in stressed group than those of control . Again in Tulsi treated group all these biochemical parameters were significantly lower (p < 0.001) than those of stressed group. This restraint stress-induced changes in body weight and biochemical parameters may be due to hypophagia, altered secretion of various metabolic hormones and neurotransmitters, changes in membrane permeability & hypovolaemia resulting from stress induced secretion of corticosterone and epinephrine through hypothalamo-pituitary adrenal axis activation. Conclusion: Prevention of stress induced changes in biochemical parameter by tulsi pre treatment indicates its anti stressor effect. Key words: Restraint stress; adaptogen;Ocimum sanctum Linn (tulsi); glucose; cholesterol; ALT; AST.   doi: 10.3329/jbsp.v3i0.1791 J Bangladesh Soc Physiol.2008 Dec;(3):29-34.  

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