scholarly journals Studies of High Molybdenum-Induced Copper Deprivation in P. przewalskii on The Qinghai Lake Pasture in China

2021 ◽  
Vol 11 (11) ◽  
pp. 5071
Author(s):  
Bowen Zhai ◽  
Kui Zhao ◽  
Fuyuan Liu ◽  
Xiaoyun Shen

The Przewalski’s Gazelles (P. przewalskii) are affected by disorders that are characterized by deprived appetites, pica, emaciation and dyskinesia. The purpose of this study was to investigate the possibility of high molybdenum (Mo) in forage leading to copper (Cu) deprivation. The mineral contents in forage, soil, and samples of hair and blood from affected ranges were compared to healthy pasture. Blood parameters were also determined. Our results showed that the mean content of Mo in forage and soil in studied pasture was 5.17 and 4.17 μg/g, respectively. The Cu to Mo ratio in forage from affected and healthy areas was 1.26 and 5.89 μg/g, respectively. The Cu concentrations in hair and blood from gazelles in affected pasture were extremely lower (p < 0.01) than those in unaffected animals. The Mo contents in hair were higher (p < 0.01) than those in unaffected gazelles. The levels of Hb, RBC, PCV, MCV, and MCH in unaffected gazelles were significantly lower (p < 0.01) than those in unaffected gazelles. The levels of TP, ALB and GLB in blood were significantly lower (p < 0.01) than those in unaffected gazelles, while the levels of AST, LDH, CPK and ALP in serum were significantly higher (p < 0.01) than those in unaffected gazelles. The activities in serum T-AOC, SOD, GSH-Px, and CAT in affected gazelles were extremely lower (p < 0.01) than those in unaffected gazelles, while MDA was significantly higher (p < 0.01) than that in unaffected gazelles. Supplementation in copper sulphate (CuSO4) has prevented and cured this disorder. In summary, high molybdenum in feed and soil may lead to secondary Cu deficiency in gazelles, which can be alleviated by supplement of copper sulfate.

Author(s):  
Rofail Rakhmanov ◽  
Elena Bogomolova ◽  
Mariya Shaposhnikova ◽  
Mariya Sapozhnikova

The biochemical blood parameters characterizing the students ’nutritional status were evaluated: protein, lipid, carbohydrate metabolism, a number of minerals. The mean values, errors of the mean, median (Me), boundary (Q) and the range of 25–75 percentiles were determined. In 9.1 % of students and 28.6 % of students, the total protein was increased. Creatinine in men was in the upper normal range, in women — at the upper limit of normal, of which 46.2 % was higher than normal. The interval Q25–75 of uric acid in students is determined in the lower normal zone. In 40.0 % of men, decreased high-density lipoprotein cholesterol (Q25–75 corresponded to 1.15–1.79), in women — below normal, Q25–75 5 was 1.3–1.5, decreased in 73.3 %. Me and Q25–75 iron were in the lower normal range; 14.1 % of men and 13.2 % of women are below normal. Me sodium and potassium at the level of the lower boundary of the norm, Q25–75 in the lower zone of the norm: in 16.0 % and 15.4 % of students the levels are reduced. Calcium is slightly above the lower limit of the norm, Q25–75–2.1–2.24, indicating an insufficient intake in the whole group; 25.0 % are below normal. The border of the 25th percentile of magnesium is at the level of the lower border of the norm, in 19.2 % it is reduced. 7.2 % lack of chlorine. Phosphorus is normal, but Q25–75 is in the upper zone; 17.9 % increased. Biochemical markers can identify individuals with metabolic disorders of nutrients. Statistical indicators — the median, the boundaries of 25–75 quartiles and their scope characterize the metabolism of macronutrients and minerals in the group and subgroups of students. Laboratory and mathematical methods can provide a basis for identifying the specific causes of these changes. For this, you can use the questionnaire method of studying the nutrition of students, possibly using the developed questionnaires for a specific situation.


2011 ◽  
pp. 323-327 ◽  
Author(s):  
Youtchou Mirabeau Tatfeng ◽  
Dennis Edo Agbonlahor

Objective: Malaria infection is severe in children who are believed to be more at risk because of their relative poor immunity against the disease. Some cytokine levels (IFN-g, IL-2, IL-4, and IL-10) of children, adolescents, and adults were assessed in this study. Methods: Cytokine levels were assayed by using Enzyme Linked Immunosorbent Assay (ELISA). Malaria diagnosis and blood parameters were carried out by using standard parasitological and haematological techniques. Results: The mean cytokine levels were significantly elevated in children, adolescent, and adult subjects when compared to their respective healthy controls (p<0.05). Also, mean IFN-g and IL-2 levels were significantly higher in children than in adults (IFN-g: 57.31±77.79 pg/ml vs. 20.37± 2.95 pg/ml, and IL-2: 108.75±63.53 pg/ml vs. 66.09±45.34 pg/ml) (p<0.05) and adolescents (IFN-g: 20.37± 2.95 pg/ml and IL-2: 66.09±45.34 pg/ml) respectively. Furthermore, mean IL-10 level was significantly lower in children (7.39±15.08 pg/ml) than mean level in adults (22.73±13.89 pg/ml). The mean haematological parameters revealed significant increase in total white blood cell, CD4, and CD8 count and significant decrease in the hematocrit of children in relation to adolescent and adult subjects (p<0.05). However, mean monocyte count was significantly higher in subjects than in their respective healthy controls (p<0.05). Conclusion: Findings in this study revealed better Th1 driven immune response in children than in adolescents and adults.


1972 ◽  
Vol 39 (3) ◽  
pp. 381-385 ◽  
Author(s):  
C. Devendra

SummaryThe variations in the composition of milk over an 18-week period from lactating British Alpine and Anglo–Nubian goats imported into Trinidad are reported. The animals were fed Napier grass (Pennisetum purpureum) ad lib. and 2 lb of a proprietary concentrate mixture daily.The mean percentage contents of the milks of British Alpine and Anglo–Nubian goats were: total solids 11·49±0·22, 12·17±0·28; butterfat 3·42±0·18, 4·06±0·22; total N 0·462±0·04, 0·530±0·07; ash 0·78±0·02, 0·79±0·03 and ‘lactose’ 4·38±0·28, 4·05±0·55. Anglo–Nubian goats had a higher content of percentage total solids, fat, total N and ash. For all constituents the coefficient of variation between weeks was higher for Anglo–Nubian goats. No significant trends were found between weeks for each of the components analysed for both breeds. Statistically significant correlations (P < 0·01) were found between percentage fat and percentage total N for both breeds.The mean mineral contents (mg/100ml) for the milks of British Alpine and Anglo–Nubian goats were: Na 52·6±4·6, 55·5±1·9; K164·0±8·4, 133·4±11·4; Ca 85·5±11·6, 89·9±9·8; Mg 13·2±3·8, 10·2±1·6 and P 74·7±3·9, 82·1±3·9. British Alpine goat's milk had a higher content of K and that of Anglo–Nubian goats had a higher content of P; both differences were statistically significant (P < 0·01).The milk composition figures in this study together with those from a similar study in Guadeloupe suggest that the values are low and considerably lower in comparison with the same breeds of goats in a temperate environment. This could be a feature of dairy goats imported into the tropics due perhaps to inadequate nutrition. Much more information is needed to verify this possibility.


2021 ◽  
Vol 103-B (3) ◽  
pp. 584-588
Author(s):  
Mohammed Khattak ◽  
Sujith Vellathussery Chakkalakumbil ◽  
Robert A. Stevenson ◽  
David J. Bryson ◽  
Michael J. Reidy ◽  
...  

Aims The aim of this study was to determine the extent to which patient demographics, clinical presentation, and blood parameters vary in Kingella kingae septic arthritis when compared with those of other organisms, and whether this difference needs to be considered when assessing children in whom a diagnosis of septic arthritis is suspected. Methods A prospective case series was undertaken at a single UK paediatric institution between October 2012 and November 2018 of all patients referred with suspected septic arthritis. We recorded the clinical, biochemical, and microbiological findings in all patients. Results A total of 160 patients underwent arthrotomy for a presumed septic arthritis. Of these, no organism was identified in 61 and only 25 of these were both culture- and polymerase chain reaction (PCR)-negative. A total of 36 patients did not undergo PCR analysis. Of the remaining 99 culture- and PCR-positive patients, K. kingae was the most commonly isolated organism (42%, n = 42). The knee (n = 21), shoulder (n = 9), and hip (n = 5) were the three most commonly affected joints. A total of 28 cases (66%) of K. kingae infection were detected only on PCR. The mean age of K. kingae-positive cases (16.1 months) was significantly lower than that of those whose septic arthitis was due to other organisms (49.4 months; p < 0.001). The mean CRP was significantly lower in the K. kingae group than in the other organism group (p < 0.001). The mean ESR/CRP ratio was significantly higher in K. kingae (2.84) than in other infections (1.55; p < 0.008). The mean ESR and ESR/CRP were not significantly different from those in the 'no organism identified' group. Conclusion K. kingae was the most commonly isolated organism from paediatric culture- and/or PCR-positive confirmed septic arthritis, with only one third of cases detected on routine cultures. It is important to develop and maintain a clinical suspicion for K. kingae infection in young patients presenting atypically. Routine PCR testing is recommended in these patients. Cite this article: Bone Joint J 2021;103-B(3):584–588.


2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Grace Otitoju ◽  
Olawale Otitoju ◽  
Paul Baiyeri ◽  
Virginia Ugwu

Abstract Objectives This study evaluated the nutrients (proximate, vitamins, minerals) and sensory attributes of some products; fried plantain buns (FPB), baked plantain buns (BPB) and steamed plantain buns (SPB) made from over-ripped plantain. Methods A bunch of matured over-ripe plantain samples (3.2 kg) were collected, washed with deionised water, peeled, mashed into pulp. About 400 g plantain pulp were mixed with other ingredients (egg 2 medium size, baking powder (14 g), vanilla flavor (0.5 ml) and onions (13 g) ), fried, steamed and baked respectively. Nutrient composition analysis was carried out in the Food Chemistry Laboratory, University of Nigeria, Nsukka, Enugu State, Nigeria. Sensory evaluations of the three products developed were done in the Food Diet Laboratory using nine-point Hedonic scale with twenty four (24) panellists. Data obtained were subjected to statistical analysis using analysis of variance (ANOVA), Standard deviation and Fisher least significance difference (FLSD) were used to compare the mean at P < 0.05. Results The proximate values of fried, baked, and steamed plantain buns were appreciable. Fat content of fried plantain buns was the highest (12.1%) while steamed plantain buns had the lowest (0.77%). Protein contents were high and comparable in all samples (P > 0.05), the highest moisture content (56.52%) was found in steamed plantain buns. The mineral content shows that Ca, Mg, Fe and Zn levels were comparable (P > 0.05) in all the samples while potassium content ranged from 342.7 to 312.0 mg/100 g with the highest level in SPB. Generally FPB has the highest mineral contents compare with BPB and SPB. Vitamin content in all the samples showed that vitamin C, vitamin A and vitamin E were present. However, there was no significant (P > 0.05) difference among the products. The general acceptability of FPB, BPB and SPB in terms of color, flavor, taste and texture were very high (8.12, 7.12 & 6.71) respectively. Although FPB and BPB were most preferred, SPB was accepted sparingly. Conclusions In conclusion, senescence plantain losses may be reduced if the over ripped ones are converted to buns products and this will consequently add to the value chain and also minimize economic loss globally. Funding Sources NIL. Supporting Tables, Images and/or Graphs


2000 ◽  
Vol 43 (2) ◽  
pp. 165-171 ◽  
Author(s):  
Elton P. Colares ◽  
Ioni G. Colares ◽  
Adalto Bianchini ◽  
Euclydes A. Santos

Seasonal variations in body weight, food consumption and blood glucose, total lipids, urea, total proteins, albumin and globulins of captive Amazonian manatees, Trichechus inunguis, were determined. Body weight changed significantly along the year, increasing from autumn to spring and decreasing in summer. The mean daily food intake of paragrass remained almost unchanged along the year. Paragrass administered to the manatees showed important variations in crude protein and lipid content along the year. No significant differences in blood parameters were registered between males and females in all seasons. Further, there were no significant differences in blood total proteins, albumin and globulins along the year. On the other hand, significant differences in the mean blood glucose, lipids and urea were registered. An increase in the blood glucose in the spring and summer was observed. Blood urea and lipids levels were positively related to paragrass protein and lipids content. These two correlations suggested that these blood parameters are good indicators of the animal nutritional status in the Amazonian manatee.


2011 ◽  
Vol 91 (3) ◽  
pp. 423-431 ◽  
Author(s):  
Leanne Van De Weyer ◽  
Cheryl Waldner

Van De Weyer, L. M. and Waldner, C. L. 2011. Geographic determinants of copper and molybdenum concentrations in serum at the end of the grazing season and associations with reproductive performance in beef cows from western Canada. Can. J. Anim. Sci. 91: 423–431. Serum concentrations of Cu and Mo were measured at the end of the summer grazing season in an observational field study of 783 breeding females from 66 cow-calf herds located in western Canada. The mean Cu concentration was 0.82 ppm (S, 0.30 ppm) and the mean Mo concentration was 0.056 ppm (S, 0.055 ppm). Serum Cu concentration differed across ecoregions (P=0.013). Serum Mo concentrations varied by ecoregion (P =0.002), soil type (P =0.011), soil color (P=0.018), and total precipitation during the growing season (P =0.004). Copper and Mo serum concentrations at the end of the grazing season were not associated with reproductive outcomes. Herd location is an important determinant of the risk of primary and secondary Cu deficiency and can inform the need for trace mineral testing of feed samples as well as supplementation.


1980 ◽  
Vol 20 (107) ◽  
pp. 717 ◽  
Author(s):  
NJ Grundon

One field and two glasshouse trials were conducted to test the effectiveness of soil and foliar applications of copper sulphate in correcting copper deficiency of wheat on a severely deficient, nearneutral clay soil in the Western Downs region of Queensland. In the field, when wheat was stressed for water from late tillering (Feekes stage 5) to anthesis (Feekes stage 11) , soil dressings of 2.5-10.0 kg CuSO4.5H2O ha-1 increased early vegetative growth but foliar symptoms of copper deficiency reappeared during stem elongation, and grain yields were negligible at all rates. A single foliar spray of 2% CuSO4.5H2O applied at mid-tillering (Feekes stage 3) also did not correct the deficiency completely: foliar symptoms reappeared and grain yield was low (141 kg ha-1). A double spray treatment, the first applied at mid-tillering and the second just before booting (Feekes stage 10), was more effective and resulted in a grain yield of 800 kg ha-1. In a glasshouse experiment supplied with adequate water, a soil dressing equivalent to 16 kg CuSO4.5H2O ha-1 (3.2 mg Culpot) completely corrected the deficiency. In a second glasshouse experiment, 2% CuSO4.5H2O solution was applied as single sprays at Feekes stages 3, 8, 9, 10 or 11, or as double sprays at Feekes stages 3 + 8, 3 + 9, 3 + 10 or 3 + 11. The most effective single spray was that applied at Feekes stage 10, but maximum dry matter and grain yield were obtained when a double spray was applied at Feekes stage 3 + 10. The effectiveness of soil and foliar applications of CuSO4.5H2O for correcting Cu deficiency of dryland wheat in Queensland soils are discussed in relation to water regime and supplying adequate Cu for both vegetative and reproductive growth.


2020 ◽  
pp. postgradmedj-2020-138667
Author(s):  
Seyma Kalkuz ◽  
Ayça Demircan

BackgroundIt has been reported that Mediterranean diet (MD) may improve quality of life and may reduce the risk of chronic diseases such as metabolic syndrome, type 2 diabetes, cardiovascular diseases (CVD), some neurodegenerative diseases and cancer. Therefore, this study aimed to determine adults’ adherence to the MD and evaluate the effect of the adherence to the MD on anthropometric measurements, specific biochemical parameters and quality of life.MethodsA total of 142 volunteer adults were included. Food frequency questionnaire, MD adherence with 14-item scale, quality of life, anthropometric measurements via bioelectrical impedance analysis and certain blood parameters were evaluated.ResultsOut of 14 points, the mean MD adherence score of the participants was 6.89. The mean fat mass was significantly higher in those with low MD adherence (p=0.024). The results of other anthropometric measurements (except height) were higher in those with low MD adherence, though results were non-significant. The participants with high adherence to the MD had lower levels of fasting blood glucose (FBG) (p=0.041), insulin (p=0.019) and triglyceride (TG) (p=0.012) compared with those with lower adherence. No significant relationship was found between the MD and quality of life and other blood parameters.ConclusionAccording to our study, MD adherence was associated with decreased fat mass, FBG, insulin and TG levels which suggests that the MD may be useful in the treatment of some chronic diseases such as obesity, diabetes, metabolic syndrome and CVD. However, more clinical trials may be performed to determine the relationship between MD and chronic diseases.


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