scholarly journals Chemical Composition of Essential Oils from Different Parts of Zingiber kerrii Craib and Their Antibacterial, Antioxidant, and Tyrosinase Inhibitory Activities

Biomolecules ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 228 ◽  
Author(s):  
Aknarin Pintatum ◽  
Surat Laphookhieo ◽  
Emilie Logie ◽  
Wim Vanden Berghe ◽  
Wisanu Maneerat
Keyword(s):  
Essential Oils ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Total Phenolic ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Diammonium Salt ◽  
Ic50 Values ◽  
Weak Antibacterial Activity ◽  
Hacat Cell Lines

The essential oils of the fresh rhizomes; flowers; and leaves of Zingiber kerrii Craib were investigated using different extraction techniques; including solid-phase microextraction (SPME), hydrodistillation (HD), and organic solvent (OS), and characterized by gas chromatography–mass spectrometry (GC–MS). A total of 37 SPME; 19 HD; and 36 OS compounds were identified from the rhizome extract of Z. kerrii; with the major components being α-pinene; β-pinene; and terpinen-4-ol; respectively. From the flower extract; 16 SPME; 2 HD; and 10 OS compounds were identified; (E)-caryophyllene was found as a major compound by these techniques. The leaf extract exhibited 20 SPME; 13 HD; and 14 OS compounds; with α-pinene; (E)-caryophyllene; and n-hexadecanoic acid being the major compounds; respectively. The rhizome extract showed tyrosinase inhibitory activity of 71.60% and a total phenolic content of 22.4 mg gallic acid/g. The IC50 values of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assays were 25.2 µg/mL and 153.6 µg/mL; respectively; and the ferric ion reducing antioxidant power (FRAP) assay value was 318.5 µM ascorbic acid equivalent (AAE)/g extract. The rhizome extract showed weak antibacterial activity. This extract showed no adverse toxicity in human keratinocyte (HaCaT) cell lines at concentrations below 200 µg/mL.

Essential oil composition, phenolic content, antioxidant, and antimicrobial activity of cultivated Satureja rechingeri Jamzad at different phenological stages

2015 ◽  
Vol 70 (3-4) ◽  
pp. 51-58 ◽  
Author(s):  
Ardalan Alizadeh
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Oil Composition ◽  
Phenological Stages ◽  
Frap Assay ◽  
Antioxidant Power

Abstract Satureja rechingeri is a rare endemic and endangered species found in Iran. Its propagation, variations in essential oil and phenolic content, as well as antioxidant and antimicrobial activities at different phenological stages are reported in this study. The chemical composition of essential oils obtained by hydro-distillation from the aerial parts were determined by GC and GC-MS. A total of 47 compounds were identified in the essential oils of S. rechingeri at different phenological stages. The major components of all oils were carvacrol (83.6%–90.4%), p-cymene (0.8%–2.9%) and γ-terpinene (0.6%–2.4%). The total phenolic content and the antioxidant activity of methanolic extracts were determined with the Folin-Ciocalteau reagent and by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and ferric reducing antioxidant power (FRAP) assay, respectively. Total phenols varied from 35.5 to 37.5 mg gallic acid equivalents/g dry weight (dw), and IC50 values in the radical scavenging assay ranged from 46.2 to 50.2 mg/mL, while those in the FRAP assay were between 49.6 and 52.5 μM quercetin equivalents/g dw. By the disc diffusion method and by determination of the minimum inhibitory concentrations (MIC), the essentials oils of the various phenological stages were found to have high activities against four medically important pathogens.

scholarly journals Influence of Cooking and Ingredients on the Antioxidant Activity, Phenolic Content and Volatile Profile of Different Variants of the Mediterranean Typical Tomato Sofrito

Antioxidants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 551 ◽  
Author(s):  
Ana Beltrán Sanahuja ◽  
Saray López De Pablo Gallego ◽  
Salvador E. Maestre Pérez ◽  
Arantzazu Valdés García ◽  
María Soledad Prats Moya
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Solid Phase ◽  
Antioxidant Properties ◽  
Gas Chromatography Mass Spectrometry ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Volatile Profile ◽  
Volatile Content ◽  
Antioxidant Power

In this study, six different sofrito formulations were compared with the raw recipe for total phenolic content (TPC), antioxidant activity tested by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric-reducing antioxidant power (FRAP) and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) methods. The volatile profile was also obtained by the headspace solid-phase microextraction-gas chromatography mass spectrometry (HS-SPME-GC–MS) procedure. The cooking process and the addition of herbs, and garlic improved the final content of antioxidant compounds compared to the basic recipe and the raw ingredients. The total volatile content was higher in the samples that contained rosemary and thymus. Some of the volatiles had proven antioxidant properties and for that reason the sofrito with rosemary with the higher volatile content was also the one with the higher antioxidant capacity and TPC. In conclusion, as well as the processing technique, the addition of selected typical Mediterranean herbs apart from given flavour can contribute to improving the nutritional antioxidant profile of dishes and be used as a natural method to increase the shelf-life of preparation.

scholarly journals ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF SELECTED PLANTS USED BY POPULATIONS FROM JURUENA VALLEY, LEGAL AMAZON, BRAZIL

2017 ◽  
Vol 9 (5) ◽  
pp. 179
Author(s):  
Larissa Irene Da Silva ◽  
Arunachalam Karuppusamy ◽  
Fabio Miyajima ◽  
Ivana Maria Povoa Violante ◽  
Isanete Geraldini Costa Bieski ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Shigella Flexneri ◽  
Antibacterial Activities ◽  
Total Phenolic ◽  
Moderate Activity ◽  
Cedrela Odorata ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Mato Grosso

Objective: The purpose of this study was to evaluate selected Brazilian plants from Juruena valley region of Mato Grosso, for their in vitro antimicrobial and antioxidant activities.Methods: The powder obtained from different parts of the twenty-six (26) plants were macerated in hydroethanolic solution to obtain the extracts. The hydroethanolic extracts were tested for their in vitro antimicrobial activity by determining the MIC using broth microdilution. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical, ferric reducing antioxidant power (FRAP) and nitric oxide (NO) methods were used for the determination of antioxidant activities. Correlation between classes of secondary metabolites and antioxidant activity was assessed.Results: Phanera glabra extract (HEPg) showed broad antibacterial spectrum, presenting the best activity against Klebsiella pneumoniae. Hevea microphylla extract (HEHm) presented a narrow spectrum of antibacterial activity with strong effect against Shigella flexneri. The only plant with broad spectrum antifungal activity was Bertholletia excelsa (HEBe), with moderate activity against strains of Aspergillus and Candida. The following extracts were prominent regarding their activities in the DPPH and FRAP assays-HEBe, Cariniana rubra (HECr) and in the FRAP assay alone, Cedrela odorata (HECo) and HEPg. None of the extracts was active in the NO assay. A significant association was observed between DPPH activity and the total phenolic contents.Conclusion: Our results justified the use of some of the investigated plants in the Brazilian ethnomedicine. The antibacterial activities of these plants are bacteriostatic in nature. These findings support that a number of investigated plants could be a valuable source of new antioxidant and antimicrobial compounds that can potentially deliver novel mechanisms of actions.However, further studies are required.

scholarly journals Effect of adding essential oils of caraway and rosemary on volatile aroma compounds derived from stored vacuum packaged minced turkey meat

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Magdalena Michalczyk ◽  
Paweł Satora ◽  
Joanna Banaś ◽  
Grzegorz Fiutak
Keyword(s):  
Beneficial Effect ◽  
Essential Oils ◽  
Solid Phase ◽  
Control Sample ◽  
Acid Value ◽  
Fat Oxidation ◽  
Viable Count ◽  
Gas Chromatography Mass Spectrometry ◽  
Total Viable Count ◽  
Turkey Meat

AbstractChanges in the odor of meat during its storage are one of the basic indicators affecting its assessment and possible disqualification. The aim of the study was to determine whether the addition of essential oils may affect the composition and concentration of volatile compounds included in the aroma of stored turkey meat. We investigated the effect of adding essential oil (EO) of caraway (0.02% v/w), rosemary (0.02% v/w) and a mixture of the two (0.01% each) on the composition of volatile compound fractions formed during 10-day storage of vacuum-packed minced turkey meat. The EOs used were also evaluated for their influence on microbial contamination (total viable count and lactic acid bacteria count), sensory quality (odor and taste) and the level of fat oxidation (acid value, peroxide value and p-anisidine value) and pH in chill-stored samples. In terms of sensory indicators, the greatest beneficial effect of adding oils was noted in the odor of raw meat. Use of the HS-SPME/GC-MS (headspace-solid phase microextraction/gas chromatography-mass spectrometry) method showed that the addition of oils significantly reduced the amounts of benzeneacetaldehyde, 2-octenal and ethyl 2-methyloctanoate compared with the control sample. In addition, in the presence of essential oils of rosemary and caraway, decreases were noted in benzaldehyde and 9-octadecenal, respectively. These changes may potentially affect the reception of the odor. There was a beneficial effect of the oils in reducing the levels of fat oxidation indicators, including peroxide. However, the oils at applied concentrations had no significant effect on the total viable count and LAB count.

scholarly journals Cotoneaster transcaucasicus Pojark. Meyve Ekstraklarında Biyoaktif Bileşen Miktarlarının ve Antioksidan Aktivitelerinin Belirlenmesi

2021 ◽  
Vol 9 (7) ◽  
pp. 1258-1263
Author(s):  
Fatma Ergün
Keyword(s):  
Methanol Extract ◽  
Antioxidant Activities ◽  
Hexane Extract ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Methanol Extracts ◽  
Ic50 Values ◽  
Phenolic Substances ◽  
Rosaceae Family ◽  
Phenolic And Flavonoid

In this study, the total phenolic and flavonoid contents and antioxidant activities of hexane and methanol extracts of Cotoneaster transcaucasicus Pojark. fruits in the Rosaceae family were investigated. Using the Folin-Ciocaltaeu method, the amount of phenolic substances was determined as 50.34 ± 2.70 mg of GAE/g in methanol extract and 38.06 ± 1.32 mg of GAE/g in hexane extract. The total amount of flavonoids was calculated as 52.01 ± 3.78 mg of QE/g in the methanol extract and 60.54 ± 6.82 mg of QE/g in the hexane extract, using the aluminum nitrate method. Antioxidant activities of C. transcaucasicus hexane and methanol extracts were evaluated by DPPH and FRAP methods. IC50 values of the extracts were calculated as 0.411 mg/mL for hexane extract and 0.237 mg/mL for methanol extract. In addition, it was determined that the reducing antioxidant power of Fe3+-Fe2+ was higher in hexane extract. As a result, it has been observed that the phenolic and flavonoid content and antioxidant activities of C. transcaucasicus fruits are high. This study is the first study in C. transcaucasicus species and it is thought that it will lead the future studies.

scholarly journals Antimicrobial and Antioxidant Activities of Different Extracts from Different Parts of Zilla spinosa (L.) Prantl

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Mohamed H. A. Suleiman ◽  
Ali A. Ateeg
Keyword(s):  
Traditional Medicine ◽  
Aerial Part ◽  
Antioxidant Activities ◽  
Shigella Flexneri ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Agar Disc

Zilla spinosa is commonly used in traditional medicine to treat gastrointestinal disorders and diabetes. In this study, aqueous ethanol (AE) and aqueous methanol (AM) extracts from aerial parts and roots of Z. spinosa were investigated. The total phenolic (TPC) and flavonoid (TFC) contents and antioxidant capacities in terms of 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging, hydrogen peroxide (H2O2), and ferric reducing antioxidant power (FRAP) assays were determined, and the correlations among the results were assessed using Pearson’s correlation. The antimicrobial activity was assessed through agar disc diffusion and broth microdilution assays. Phytochemical screening showed that Z. spinosa extracts had alkaloids, glycosides, saponins, triterpenoids, phenols, and flavonoids in different abundances. The aerial part-AE extract contained low TPC (30.17 ± 4.24 mg GAE/g) and TFC (7.40 ± 1.02 mg QE/g) and displayed significant antioxidant capacity in the DPPH (IC50 = 52.17 ± 7.30 μg/mL), H2O2 (91.22 ± 2.60 μg/mL), and FRAP (EC50 = 98.70 ± 2.21 μg/mL) assays. By contrast, the root-AM extract contained high amounts of TPC (87.72 ± 7.75 mg GAE/g) and TFC (25.60 ± 1.57 mg QE/g). It showed significantly high antioxidant activity with IC50 values of 12.33 ± 1.88 μg/mL in the DPPH and 39.37 ± 2.59 μg/mL in the H2O2 assays, as well as reducing power capacity with an EC50 value of 20.82 ± 1.14 μg/mL in the FRAP assay. Both TPC and TFC were exhibited negative correlations ( p < 0.01 ) with the IC50 and EC50 values obtained in the applied antioxidant assays. The aerial part-AM extract showed the highest inhibitory activity against Staphylococcus aureus (26.5 ± 0.20 mm), followed by Shigella flexneri (19.4 ± 0.40 mm) and Proteus mirabilis (17.7 ± 0.49 mm). S. aureus was the most affected microorganism with a minimum inhibitory concentration of 128 μg/mL against the aerial part-AM extract. Interestingly, all evaluated extracts showed potent antifungal activity against Candida albicans. However, aerial part-AM was the most effective, with an inhibition zone of 12.6 ± 0.17 mm. The results concluded that Z. spinosa possesses different phytochemicals displaying significant antioxidant and antimicrobial activities, thus lending credence to its use in traditional medicine.

scholarly journals Chemical Composition, Antioxidant, Anti-Tyrosinase, Anti-Cholinesterase and Cytotoxic Activities of Essential Oils of Six Algerian Plants

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1710 ◽  
Author(s):  
Kadour Cheraif ◽  
Boulanouar Bakchiche ◽  
Abdelaziz Gherib ◽  
Sanaa K. Bardaweel ◽  
Melek Çol Ayvaz ◽  
...  
Keyword(s):  
Essential Oils ◽  
Cytotoxic Effect ◽  
Food Preservation ◽  
Major Constituent ◽  
Cytotoxic Activities ◽  
Mentha Pulegium ◽  
Antioxidant Power ◽  
Ic50 Values ◽  
Lavandula Officinalis ◽  
Main Component

In this study, the essential oils (EOs) of six Algerian plants (Artemisia campestris L., Artemisia herba-alba Asso, Juniperus phoenicea L., Juniperus oxycedrus L., Mentha pulegium L. and Lavandula officinalis Chaix) were obtained by hydrodistillation, and their compositions determined by GC-MS and GC-FID. The antioxidant activity of the EOS was evaluated via 2,2′-diphenyl-1-picrylhydrazyl (DPPH), ferric-reducing/antioxidant power (FRAP) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assays. Moreover, their cytotoxic effect was evaluated—as well as their tyrosinase, acetyl- and butyryl-cholinesterase (AChE and BuChE) inhibitory activities. The chemical analyses detected 44, 45, 51, 53, 26 and 40 compounds in EOs of A. campestris, A. herba-alba, J. phoenicea, J. oxycedrus, M. pulegium and L. officinalis, respectively. A. campestris EO was mainly composed of β-pinene (20.7%), while A. herba-alba EO contained davanone D (49.5%) as the main component. α-Pinene (41.8%) was detected as the major constituent in both J. phoenicea (41.8%) and J. oxycedrus (37.8%) EOs. M. pulegium EO was characterized by pulegone as the most abundant (76.9%) compound, while linalool (35.8%) was detected as a major constituent in L. officinalis EO. The antioxidant power evaluation revealed IC50 values ranging from 2.61 to 91.25 mg/mL for DPPH scavenging activity, while the FRAP values ranged from 0.97–8.17 µmol Trolox equivalents (TX)/g sample. In the ABTS assay, the values ranged from 7.01 to 2.40 µmol TX/g sample. In the presence of 1 mg/mL of the samples, tyrosinase inhibition rates ranged from 11.35% to 39.65%, AChE inhibition rates ranged from 40.57% to 73.60% and BuChE inhibition rates ranged from 6.47% to 72.03%. A significant cytotoxic effect was found for A. herba-alba EO. The obtained results support some of the traditional uses of these species in food preservation and for protection against several diseases.

scholarly journals Detection and Identification of Antibacterial and Antioxidant Components of Essential Oils by TLC-Biodetection and GC-MS

2016 ◽  
Vol 11 (11) ◽  
pp. 1934578X1601101 ◽  
Author(s):  
Ágnes M. Móricz ◽  
Györgyi Horváth ◽  
Andrea Böszörményi ◽  
Péter G. Ott
Keyword(s):  
Essential Oils ◽  
Solid Phase ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Cinnamon Bark ◽  
Scavenging Effect ◽  
Detection And Identification ◽  
Radical Scavenging Effect ◽  
Direct Bioautography ◽  
Layer Chromatography

Components of cinnamon bark, rosemary, clove and thyme essential oils were screened for antioxidant and antibacterial activity utilizing thin-layer chromatography (TLC) coupled with the DPPH• test and direct bioautography using Bacillus subtilis cells. The compounds in the active chromatographic zones were identified by solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) after their elution. Seven antibacterial components were found: cinnamaldehyde and eugenol in cinnamon bark oil, 1,8-cineole, camphor, borneol and α-terpineol in rosemary oil, eugenol in clove oil and thymol in thyme oil. Only two of them, thymol and eugenol displayed a free radical scavenging effect.

scholarly journals Potential Application of Prunus armeniaca L. and P. domestica L. Leaf Essential Oils as Antioxidant and of Cholinesterases Inhibitors

Antioxidants ◽  
2018 ◽  
Vol 8 (1) ◽  
pp. 2 ◽  
Author(s):  
Marco Bonesi ◽  
Maria Tenuta ◽  
Monica Loizzo ◽  
Vincenzo Sicari ◽  
Rosa Tundis
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Antioxidant Properties ◽  
Positive Control ◽  
Prunus Armeniaca ◽  
Gas Chromatography Mass Spectrometry ◽  
Flame Ionization ◽  
Ic50 Values ◽  
Comprehensive Comparison

The aim of this work is to investigate the in vitro acetylcholinesterase (AChE) and butyrycholinesterase (BChE) inhibitory activities of essential oils obtained by hydrodistillation from the leaves of Prunus armeniaca and P. domestica in relation to their composition, analysed by Gas Chromatography–Flame Ionization Detector (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS) analyses, at different times. Moreover, considering the role of free radicals in the progression of neurodegenerative disorders, the antioxidant properties of essential oils were investigated by using, 2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and β-carotene bleaching tests. The relative antioxidant capacity index (RACI) was used to achieve more comprehensive comparison between analysed antioxidant effects of essential oils. P. armeniaca oils were more active than P. domestica oils against AChE. Against BChE, the most active was the essential oil from P. domestica leaves collected in August with an IC50 value of 95.80 μg/mL. This oil exerted the highest inhibitory activity of lipid peroxidation with IC50 values of 11.15 and 11.39 μg/mL after 30 and 60 min of incubation, respectively. All samples demonstrated a remarkable ABTS radicals scavenging activity, with IC50 values in the range 0.45–0.57 μg/mL in comparison to the positive control, ascorbic acid.

scholarly journals Improved Antioxidant, Anti-inflammatory, and Anti-adipogenic Properties of Hydroponic Ginseng Fermented by Leuconostoc mesenteroides KCCM 12010P

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3359 ◽  
Author(s):  
Ji Eun Hwang ◽  
Kee-Tae Kim ◽  
Hyun-Dong Paik
Keyword(s):  
Nitric Oxide ◽  
Leuconostoc Mesenteroides ◽  
Raw 264.7 Cells ◽  
Total Phenolic ◽  
Oxide Content ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Anti Inflammatory ◽  
Factor Α

Hydroponic ginseng (HPG) has been known to have various bio-functionalities, including an antioxidant effect. Recently, fermentation by lactic acid bacteria has been studied to enhance bio-functional activities in plants by biologically converting their chemical compounds. HPG roots and shoots were fermented with Leuconostoc mesenteroides KCCM 12010P isolated from kimchi. The total phenolic compounds, antioxidant, anti-inflammatory, and anti-adipogenic effects of these fermented samples were evaluated in comparison with non-fermented samples (control). During 24 h fermentation of HPG roots and shoots, the viable number of cells increased to 7.50 Log colony forming unit (CFU)/mL. Total phenolic and flavonoid contents of the fermented HPG roots increased by 107.19% and 645.59%, respectively, compared to non-fermented HPG roots. The antioxidant activity of fermented HPG, as assessed by 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), β-carotene-linoleic, and ferric reducing antioxidant power (FRAP) assay, was also significantly enhanced. In an anti-inflammatory effect of lipopolysaccharide (LPS)-stimulated RAW 264.7 cells, the nitric oxide content and the expression of inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) decreased when treated with fermented samples. Simultaneously, lipid accumulation in 3T3-L1 adipocyte was reduced when treated with fermented HPG. Fermentation by L. mesenteroides showed improved antioxidant, anti-inflammatory and anti-adipogenic HPG effects. These results show that fermented HPG has potential for applications in the functional food industry.

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