scholarly journals ICP-Mass-Spectrometry Ionic Profile of Whole Saliva in Patients with Untreated and Treated Periodontitis

Biomedicines ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 354
Author(s):  
Federica Romano ◽  
Alexandra Castiblanco ◽  
Francesca Spadotto ◽  
Federica Di Scipio ◽  
Mery Malandrino ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Cross Sectional Study ◽  
Mineral Elements ◽  
Healthy Controls ◽  
Cross Sectional ◽  
Inductively Coupled ◽  
Severe Periodontitis ◽  
Whole Saliva ◽  
Icp Mass Spectrometry

Over the past decade, there has been growing interest in the association between macro and trace minerals in body fluids and systemic diseases related to chronic inflammation and oxidative stress. Due to the paucity of data in the literature on periodontitis, the aim of this cross-sectional study was to assess the relationship between mineral elements in saliva and periodontal status in patients with untreated and treated periodontitis compared to periodontally healthy controls. Salivary samples from 66 nonsmoker healthy patients (20 periodontally healthy, 24 untreated severe periodontitis and 22 treated severe periodontitis) were analyzed by using inductively coupled plasma mass-spectrometry (ICP-MS). Significant increases in copper (Cu), sodium (Na), iron (Fe) and manganese (Mn) concentrations occurred in saliva of severe periodontitis subjects compared to periodontally healthy controls. No differences were detected between healthy controls and treated periodontitis patients apart from levels of zinc (Zn) and lithium (Li) that were found to be increased and reduced, respectively, in periodontitis group. Most subjects were correctly separated by cluster analysis into active periodontitis and periodontally healthy individuals. Treated periodontitis individuals were classified as healthy subjects. Based on these preliminary results, the assessment of salivary concentration of mineral elements might be useful in discriminating periodontal health and disease.

Estimation of Trace Elements in Gingival Crevicular Fluid and Serum-Comparative Study in Healthy and Periodontitis

2017 ◽  
Vol 9 (5) ◽  
Author(s):  
Bhagavathami Meenaksh ◽  
Chitraa R. Chandran ◽  
Aravindhan Thathchari Ranganathan ◽  
Kavindapadi Venkateswaran Rama ◽  
Valarmathy Srinivasan
Keyword(s):  
Trace Elements ◽  
Inductively Coupled Plasma ◽  
Gingival Crevicular Fluid ◽  
Serum Zinc ◽  
Cross Sectional Study ◽  
Serum Copper ◽  
Cross Sectional ◽  
Inductively Coupled ◽  
Significant Difference ◽  
Copper Zinc

Background: This study aims to determine and compare the levels of trace elements copper, zinc, selenium and chromium in GCF and serum of patients with periodontitis and healthy individuals. Methods: This cross sectional study includes 24 study subjects recruited from the patients reporting to the Department of Periodontics , Tagore Dental College Chennai. All the selected patients were subjected to a clinical examination done by a single examiner. The estimation of trace elements Copper, Zinc, Selenium and Chromium in GCF and serum is performed using Perkin Elmer optima 5300 Inductively Coupled Plasma Emission Spectrometer (ICPOES). Results: GCF and serum copper levels showed no significant difference in both periodontitis and healthy groups . Selenium levels tend to be the same in both groups. Serum zinc levels are more in periodontitis patients than healthy subjects (p less than 0.01). GCF chromium levels are found to be more in patients with periodontitis than healthy. Conclusions : More research is therefore needed to monitor the role of these trace elements C with an increased sample size to ascertain whether they are associated with a reduced risk of periodontitis.

scholarly journals Nanoplastic Labelling with Metal Probes: Analytical Strategies for Their Sensitive Detection and Quantification by ICP Mass Spectrometry

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7093
Author(s):  
Lucile Marigliano ◽  
Bruno Grassl ◽  
Joanna Szpunar ◽  
Stéphanie Reynaud ◽  
Javier Jiménez-Lamana
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Analytical Techniques ◽  
Inductively Coupled ◽  
Advantages And Disadvantages ◽  
Promising Tool ◽  
Icp Ms ◽  
Analytical Strategies ◽  
Icp Mass Spectrometry ◽  
Detection And Quantification

The detection and quantification of nanoplastics in aquatic environments is one of the major challenges in environmental and analytical research nowadays. The use of common analytical techniques for this purpose is not only hampered by the size of nanoplastics, but also because they are mainly made of carbon. In addition, the expected concentrations in environmental samples are below the detection limit of the majority of analytical techniques. In this context, the great detection capabilities of Inductively Coupled Plasma Mass Spectrometry (ICP-MS) in its Single Particle mode (SP-ICP-MS) have made of this technique a good candidate for the analysis of nanoplastics. Since the monitoring of carbon by ICP-MS faces several difficulties, the use of metal tags, taking advantage of the great potential of nanoplastics to adsorb chemical compounds, has been proposed as an alternative. In this perspectives paper, three different strategies for the analysis of polystyrene (PS) nanoplastics by SP-ICP-MS based on the use of metals species (ions, hydrophobic organometallic compound, and nanoparticles) as tags are presented and discussed. Advantages and disadvantages of each strategy, which rely on the labelling process, are highlighted. The metal nanoparticles labelling strategy is shown as a promising tool for the detection and quantification of nanoplastics in aqueous matrices by SP-ICP-MS.

scholarly journals Quantitative Evaluation of 28 Mineral Elements by Inductively Coupled Plasma/Mass Spectrometry and Its Application in Source Identification of Indian Opium

2005 ◽  
Vol 88 (5) ◽  
pp. 1469-1484 ◽  
Author(s):  
Mohanakrishna R Mudiam ◽  
Sanjukta A Kumar ◽  
Sudhershanan Mahadevan ◽  
Priyankar Ghosh ◽  
Rajendra K Sarin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Analytical Data ◽  
Mineral Elements ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Geographical Regions ◽  
Plasma Mass Spectrometry ◽  
Elemental Data

Abstract An analytical method based on inductively coupled plasma/mass spectrometry (ICP/MS) was developed for the determination of 28 mineral elements (Cr, Mn, Co, Ni, Cu, Zn, Ga, Sr, Cd, Ag, Ba, Pb, Bi Y, La, Ce, Nd, Pr, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, and Lu) in Indian opium samples. The detection limits were found to be in the range of 0.0008–0.45 ng/mL. The recoveries of spiked samples for each element were found to be in the range of 83–106%, with a precision of less than 9%. A total of 124 opium samples from India were analyzed for the distribution pattern of the 28 mineral elements. Quantitative elemental data were subjected to chemometric analysis in order to determine an optimal classifier to evaluate the source of Indian opium. The study indicated that mineral elements might not be the suitable discriminators for the discrimination of licit opium-growing divisions of India. However, the methodology developed and the analytical data on elemental profile may find important forensic application in discriminating Indian opium with that of licit and illicit opium originating from different geographical regions of world.

A More Accurate Molar Mass of Silicon via High Resolution MC-ICP-Mass Spectrometry

2014 ◽  
Vol 228 (4-5) ◽  
Author(s):  
Axel Pramann ◽  
Olaf Rienitz ◽  
Janine Noordmann ◽  
Bernd Güttler ◽  
Detlef Schiel
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Inductively Coupled Plasma ◽  
Molar Mass ◽  
Silicon Crystal ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Icp Mass Spectrometry

AbstractHigh resolution multicollector inductively coupled plasma mass spectrometry (HR-MC-ICP-MS) was applied for the determination of the isotopic composition and molar mass of a silicon crystal material (“Si28”) highly enriched in the

scholarly journals Investigation of Salivary RANKL and OPG Levels in Periodontitis Patients at Hospital Universiti Sains Malaysia

2021 ◽  
Author(s):  
Saba Asif ◽  
Basaruddin Ahmad ◽  
Syed Ameer Hamza ◽  
Haslina Taib ◽  
Nur Karyatee Kassim ◽  
...  
Keyword(s):  
Cross Sectional Study ◽  
Periodontal Pocket ◽  
Pocket Depth ◽  
Cross Sectional ◽  
Plaque Score ◽  
Mean Values ◽  
Severe Periodontitis ◽  
Whole Saliva ◽  
Elisa Technique ◽  
Unstimulated Whole Saliva

Abstract Background This study was aimed to determine the levels of salivary receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) and its association with periodontal status among periodontitis patients. Patients and Methods A cross-sectional study was designed and performed at the Dental Clinic, Hospital Universiti Sains Malaysia (HUSM). Random sampling was employed to identify 88 participants into three groups: 30 mild periodontitis, 30 moderate to severe periodontitis, and 28 healthy (nonperiodontitis) patients. Periodontal parameters: periodontal pocket depth (PPD), clinical attachment level (CAL), plaque score (PS), and gingival bleeding index (GBI) were recorded. In total, 4 mL of unstimulated whole saliva was collected to determine the levels of salivary RANKL and OPG proteins by using ELISA technique. Data were analyzed by using SPSS software version 24.0. Results Mean values for PPD (5.3 ± 0.5) and CAL (5.6 ± 0.5) were observed higher for moderate to severe periodontitis as compared with values (4.4 ± 0.2) (4.5 ± 0.2) in mild periodontitis patients. The mean salivary RANKL and OPG was 0.23 ± 0.07 ng/mL and 1.78 ± 0.70 ng/mL respectively in moderate to severe periodontitis. Only salivary RANKL levels were significantly and positively correlated with all the clinical periodontal parameters. Conclusion The levels of salivary RANKL were higher as opposed to lower OPG levels in periodontitis patients in contrast to healthy (nonperiodontitis) patients. RANKL levels were significantly associated with the periodontal parameters. Therefore, we can conclude that RANKL can potentially aid as an adjunctive diagnostic protein in evaluating periodontal disease.

scholarly journals Assessing the suitability of 8-OHdG and micronuclei as genotoxic biomarkers in chromate-exposed workers: a cross-sectional study

BMJ Open ◽  
2014 ◽  
Vol 4 (10) ◽  
pp. e005979 ◽  
Author(s):  
Ping Li ◽  
Yongen Gu ◽  
Shanfa Yu ◽  
Yang Li ◽  
Jinglin Yang ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Micronucleus Test ◽  
Cross Sectional Study ◽  
Exposed Group ◽  
Control Group ◽  
Sectional Study ◽  
Cross Sectional ◽  
Positive Correlation ◽  
Inductively Coupled ◽  
Positive Correlations

ObjectivesWe aimed to investigate suitable conditions of 8-hydroxy-2′-deoxyguanosine (8-OHdG) and micronucleus (MN) as genotoxic biomarkers at different levels of occupational chromate exposure.DesignA cross-sectional study was used.Participants84 workers who were exposed to chromate for at least 1 year were chosen as the chromate exposed group, while 30 non-exposed individuals were used as controls.Main outcome measuresEnvironmental and biological exposure to chromate was respectively assessed by measuring the concentration of chromate in the air (CrA) and blood (CrB) by inductively coupled plasma mass spectrometer (ICP-MS) in all participants. MN indicators, including micronucleus cell count (MNCC), micro-nucleus count (MNC), nuclear bridge (NPB) and nuclear bud (NBUD) were calculated by the cytokinesis-block micronucleus test (CBMN), while the urinary 8-OHdG was measured by the ELISA method and normalised by the concentration of Cre.ResultsCompared with the control group, the levels of CrA, CrB, MNCC, MNC and 8-OHdG in the chromate-exposed group were all significantly higher (p<0.05). There were positive correlations between log(8-OHdG) and LnMNCC or LnMNC (r=0.377 and 0.362). The levels of LnMNCC, LnMNC and log (8-OHdG) all have parabola correlations with the concentration of CrB. However, there was a significantly positive correlation between log (8-OHdG) and CrB when the CrB level was below 10.50 µg/L (r=0.355), while a positive correlation was also found between LnMNCC or LnMNC and CrB when the CrB level was lower than 9.10 µg/L (r=0.365 and 0.269, respectively).ConclusionsMN and 8-OHdG can be used as genotoxic biomarkers in the chromate-exposed group, but it is only when CrB levels are lower than 9.10 and 10.50 µg/L, respectively, that they can accurately reflect the degree of genetic damage.

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