Recent Advances in Cardiac Tissue Engineering for the Management of Myocardium Infarction

Myocardium Infarction (MI) is one of the foremost cardiovascular diseases (CVDs) causing death worldwide, and its case numbers are expected to continuously increase in the coming years. Pharmacological interventions have not been at the forefront in ameliorating MI-related morbidity and mortality. Stem cell-based tissue engineering approaches have been extensively explored for their regenerative potential in the infarcted myocardium. Recent studies on microfluidic devices employing stem cells under laboratory set-up have revealed meticulous events pertaining to the pathophysiology of MI occurring at the infarcted site. This discovery also underpins the appropriate conditions in the niche for differentiating stem cells into mature cardiomyocyte-like cells and leads to engineering of the scaffold via mimicking of native cardiac physiological conditions. However, the mode of stem cell-loaded engineered scaffolds delivered to the site of infarction is still a challenging mission, and yet to be translated to the clinical setting. In this review, we have elucidated the various strategies developed using a hydrogel-based system both as encapsulated stem cells and as biocompatible patches loaded with cells and applied at the site of infarction.

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Immune response to stem cells and strategies to induce tolerance

Philosophical Transactions of the Royal Society B Biological Sciences ◽

10.1098/rstb.2007.2120 ◽

2007 ◽

Vol 362 (1484) ◽

pp. 1343-1356 ◽

Cited By ~ 10

Author(s):

Puspa Batten ◽

Nadia A Rosenthal ◽

Magdi H Yacoub

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Stem Cell ◽

Cell Biology ◽

Heart Valves ◽

Cardiac Tissue ◽

Full Potential ◽

Great Expectations ◽

Cardiovascular Tissue ◽

Form And Function

Although recent progress in cardiovascular tissue engineering has generated great expectations for the exploitation of stem cells to restore cardiac form and function, the prospects of a common mass-produced cell resource for clinically viable engineered tissues and organs remain problematic. The refinement of stem cell culture protocols to increase induction of the cardiomyocyte phenotype and the assembly of transplantable vascularized tissue are areas of intense current research, but the problem of immune rejection of heterologous cell type poses perhaps the most significant hurdle to overcome. This article focuses on the potential advantages and problems encountered with various stem cell sources for reconstruction of the damaged or failing myocardium or heart valves and also discusses the need for integrating advances in developmental and stem cell biology, immunology and tissue engineering to achieve the full potential of cardiac tissue engineering. The ultimate goal is to produce ‘off-the-shelf’ cells and tissues capable of inducing specific immune tolerance.

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Promising Therapeutic Strategies for Mesenchymal Stem Cell-Based Cardiovascular Regeneration: From Cell Priming to Tissue Engineering

Stem Cells International ◽

10.1155/2017/3945403 ◽

2017 ◽

Vol 2017 ◽

pp. 1-13 ◽

Cited By ~ 17

Author(s):

Seung Taek Ji ◽

Hyunyun Kim ◽

Jisoo Yun ◽

Joo Seop Chung ◽

Sang-Mo Kwon

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Stem Cell ◽

Cardiovascular Diseases ◽

Therapeutic Strategy ◽

Therapeutic Strategies ◽

Cell Protein ◽

Ischemic Lesion ◽

Cardiovascular Regeneration ◽

A Cell

The primary cause of death among chronic diseases worldwide is ischemic cardiovascular diseases, such as stroke and myocardial infarction. Recent evidence indicates that adult stem cell therapies involving cardiovascular regeneration represent promising strategies to treat cardiovascular diseases. Owing to their immunomodulatory properties and vascular repair capabilities, mesenchymal stem cells (MSCs) are strong candidate therapeutic stem cells for use in cardiovascular regeneration. However, major limitations must be overcome, including their very low survival rate in ischemic lesion. Various attempts have been made to improve the poor survival and longevity of engrafted MSCs. In order to develop novel therapeutic strategies, it is necessary to first identify stem cell modulators for intracellular signal triggering or niche activation. One promising therapeutic strategy is the priming of therapeutic MSCs with stem cell modulators before transplantation. Another is a tissue engineering-based therapeutic strategy involving a cell scaffold, a cell-protein-scaffold architecture made of biomaterials such as ECM or hydrogel, and cell patch- and 3D printing-based tissue engineering. This review focuses on the current clinical applications of MSCs for treating cardiovascular diseases and highlights several therapeutic strategies for promoting the therapeutic efficacy of MSCs in vitro or in vivo from cell priming to tissue engineering strategies, for use in cardiovascular regeneration.

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Hard Dental Tissues Regeneration—Approaches and Challenges

Materials ◽

10.3390/ma14102558 ◽

2021 ◽

Vol 14 (10) ◽

pp. 2558

Author(s):

Mihaela Olaru ◽

Liliana Sachelarie ◽

Gabriela Calin

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Stem Cell ◽

Growth Factors ◽

Present Review ◽

Modern Concept ◽

Dental Tissues ◽

Tissue Engineering Approach ◽

Hard Dental Tissues ◽

Review Reports

With the development of the modern concept of tissue engineering approach and the discovery of the potential of stem cells in dentistry, the regeneration of hard dental tissues has become a reality and a priority of modern dentistry. The present review reports the recent advances on stem-cell based regeneration strategies for hard dental tissues and analyze the feasibility of stem cells and of growth factors in scaffolds-based or scaffold-free approaches in inducing the regeneration of either the whole tooth or only of its component structures.

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Application potential of three-dimensional silk fibroin scaffold using mesenchymal stem cells for cardiac regeneration

Journal of Biomaterials Applications ◽

10.1177/08853282211018529 ◽

2021 ◽

pp. 088532822110185

Author(s):

Yuksel Cetin ◽

Merve G Sahin ◽

Fatma N Kok

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Silk Fibroin ◽

Cardiac Tissue ◽

Troponin I ◽

Cardiac Tissue Engineering ◽

Swelling Ratio ◽

Differentiation Potential ◽

Cardiomyogenic Differentiation

Cardiac tissue engineering focusing on biomaterial scaffolds incorporating cells from different sources has been explored to regenerate or repair damaged area as a lifesaving approach.The aim of this study was to evaluate the cardiomyocyte differentiation potential of human adipose mesenchymal stem cells (hAD-MSCs) as an alternative cell source on silk fibroin (SF) scaffolds for cardiac tissue engineering. The change in surface morphology of SF scaffolds depending on SF concentration (1–6%, w/v) and increase in their porosity upon application of unidirectional freezing were visualized by scanning electron microscopy (SEM). Swelling ratio was found to increase 2.4 fold when SF amount was decreased from 4% to 2%. To avoid excessive swelling, 4% SF scaffold with swelling ratio of 10% (w/w) was chosen for further studies.Biodegradation rate of SF scaffolds depended on enzymatic activity was found to be 75% weight loss of SF scaffolds at the day 14. The phenotype of hAD-MSCs and their multi-linage potential into chondrocytes, osteocytes, and adipocytes were shown by flow cytometry and immunohistochemical staining, respectively.The viability of hAD-MSCs on 3D SF scaffolds was determined as 90%, 118%, and 138% after 1, 7, and 14 days, respectively. The use of 3D SF scaffolds was associated with increased production of cardiomyogenic biomarkers: α-actinin, troponin I, connexin 43, and myosin heavy chain. The fabricated 3D SF scaffolds were proved to sustain hAD-MSCs proliferation and cardiomyogenic differentiation therefore, hAD-MSCs on 3D SF scaffolds may useful tool to regenerate or repair damaged area using cardiac tissue engineering techniques.

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Fiber alignment and coculture with fibroblasts improves the differentiated phenotype of murine embryonic stem cell-derived cardiomyocytes for cardiac tissue engineering

Biotechnology and Bioengineering ◽

10.1002/bit.23353 ◽

2011 ◽

Vol 109 (3) ◽

pp. 813-822 ◽

Cited By ~ 76

Author(s):

Ian C. Parrag ◽

Peter W. Zandstra ◽

Kimberly A. Woodhouse

Keyword(s):

Tissue Engineering ◽

Stem Cell ◽

Embryonic Stem Cell ◽

Cardiac Tissue ◽

Embryonic Stem ◽

Cardiac Tissue Engineering ◽

Fiber Alignment ◽

Murine Embryonic Stem Cell

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Magnetic field assisted stem cell differentiation – role of substrate magnetization in osteogenesis

Journal of Materials Chemistry B ◽

10.1039/c5tb00118h ◽

2015 ◽

Vol 3 (16) ◽

pp. 3150-3168 ◽

Cited By ~ 29

Author(s):

Sunil Kumar Boda ◽

Greeshma Thrivikraman ◽

Bikramjit Basu

Keyword(s):

Magnetic Field ◽

Tissue Engineering ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Stem Cell ◽

Cell Differentiation ◽

Bone Tissue Engineering ◽

Human Mesenchymal Stem Cells ◽

Stem Cell Differentiation

Substrate magnetization as a tool for modulating the osteogenesis of human mesenchymal stem cells for bone tissue engineering applications.

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Artificial Cardiac Muscle with or without the Use of Scaffolds

BioMed Research International ◽

10.1155/2017/8473465 ◽

2017 ◽

Vol 2017 ◽

pp. 1-15 ◽

Cited By ~ 4

Author(s):

Yifei Li ◽

Donghui Zhang

Keyword(s):

Tissue Engineering ◽

Cardiovascular Diseases ◽

Cardiac Tissue ◽

Contractile Function ◽

Rapid Development ◽

Treatment Options ◽

Cardiac Tissue Engineering ◽

Hydrogel Scaffold ◽

Fibrous Matrix ◽

Advantages And Disadvantages

During the past several decades, major advances and improvements now promote better treatment options for cardiovascular diseases. However, these diseases still remain the single leading cause of death worldwide. The rapid development of cardiac tissue engineering has provided the opportunity to potentially restore the contractile function and retain the pumping feature of injured hearts. This conception of cardiac tissue engineering can enable researchers to produce autologous and functional biomaterials which represents a promising technique to benefit patients with cardiovascular diseases. Such an approach will ultimately reshape existing heart transplantation protocols. Notable efforts are accelerating the development of cardiac tissue engineering, particularly to create larger tissue with enhanced functionality. Decellularized scaffolds, polymer synthetics fibrous matrix, and natural materials are used to build robust cardiac tissue scaffolds to imitate the morphological and physiological patterns of natural tissue. This ultimately helps cells to implant properly to obtain endogenous biological capacity. However, newer designs such as the hydrogel scaffold-free matrix can increase the applicability of artificial tissue to engineering strategies. In this review, we summarize all the methods to produce artificial cardiac tissue using scaffold and scaffold-free technology, their advantages and disadvantages, and their relevance to clinical practice.

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Use of adipose-derived stem cells in lymphatic tissue engineering and regeneration

Archives of Plastic Surgery ◽

10.5999/aps.2021.00339 ◽

2021 ◽

Vol 48 (5) ◽

pp. 559-567

Author(s):

Antonio Jorge Forte ◽

Daniel Boczar ◽

Rachel Sarabia-Estrada ◽

Maria T. Huayllani ◽

Francisco R. Avila ◽

...

Keyword(s):

Breast Cancer ◽

Systematic Review ◽

Tissue Engineering ◽

Stem Cells ◽

Stem Cell ◽

Growth Factor ◽

Cochrane Central Register ◽

Adipose Derived Stem Cells ◽

Lymphatic Tissue ◽

Factor C

The potential to differentiate into different cell lines, added to the easy and cost-effective method of extraction, makes adipose-derived stem cells (ADSCs) an object of interest in lymphedema treatment. Our study’s goal was to conduct a comprehensive systematic review of the use of ADSCs in lymphatic tissue engineering and regeneration. On July 23, 2019, using PubMed/MEDLINE, Cochrane Clinical Answers, Cochrane Central Register of Controlled Trials, and Embase databases, we conducted a systematic review of published literature on the use of ADSCs in lymphatic tissue engineering and regeneration. There were no language or time frame limitations, and the following search strategy was applied: ((Adipose stem cell) OR Adipose-derived stem cell)) AND ((Lymphedema) OR Breast Cancer Lymphedema). Only original research manuscripts were included. Fourteen studies fulfilled the inclusion criteria. Eleven studies were experimental (in vitro or in vivo in animals), and only three were clinical. Publications on the topic demonstrated that ADSCs promote lymphangiogenesis, and its effect could be enhanced by modulation of vascular endothelial growth factor-C, interleukin-7, prospero homeobox protein 1, and transforming growth factor-β1. Pilot clinical studies included 11 patients with breast cancer-related lymphedema, and no significant side effects were present at 12-month follow-up. Literature on the use of ADSCs in lymphatic tissue engineering and regeneration demonstrated promising data. Clinical evidence is still in its infancy, but the scientific community agrees that ADSCs can be useful in regenerative lymphangiogenesis. Data collected in this review indicate that unprecedented advances in lymphedema treatment can be anticipated in the upcoming years.

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Biocompatible succinic acid-based polyesters for potential biomedical applications: fungal biofilm inhibition and mesenchymal stem cell growth

RSC Advances ◽

10.1039/c5ra15858c ◽

2015 ◽

Vol 5 (104) ◽

pp. 85756-85766 ◽

Cited By ~ 12

Author(s):

E. Jäger ◽

R. K. Donato ◽

M. Perchacz ◽

A. Jäger ◽

F. Surman ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Stem Cell ◽

Cell Growth ◽

Mesenchymal Stem Cell ◽

Medical Devices ◽

Biomedical Applications ◽

Growth Promotion ◽

Intrinsic Properties ◽

Biofilm Inhibition

Poly(alkene succinates) are promising materials for specialized medical devices and tissue engineering, presenting intrinsic properties, such as; fungal biofilm inhibition, biocompatibility and stem cells controlled growth promotion.

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