Promising Therapeutic Strategies for Mesenchymal Stem Cell-Based Cardiovascular Regeneration: From Cell Priming to Tissue Engineering

The primary cause of death among chronic diseases worldwide is ischemic cardiovascular diseases, such as stroke and myocardial infarction. Recent evidence indicates that adult stem cell therapies involving cardiovascular regeneration represent promising strategies to treat cardiovascular diseases. Owing to their immunomodulatory properties and vascular repair capabilities, mesenchymal stem cells (MSCs) are strong candidate therapeutic stem cells for use in cardiovascular regeneration. However, major limitations must be overcome, including their very low survival rate in ischemic lesion. Various attempts have been made to improve the poor survival and longevity of engrafted MSCs. In order to develop novel therapeutic strategies, it is necessary to first identify stem cell modulators for intracellular signal triggering or niche activation. One promising therapeutic strategy is the priming of therapeutic MSCs with stem cell modulators before transplantation. Another is a tissue engineering-based therapeutic strategy involving a cell scaffold, a cell-protein-scaffold architecture made of biomaterials such as ECM or hydrogel, and cell patch- and 3D printing-based tissue engineering. This review focuses on the current clinical applications of MSCs for treating cardiovascular diseases and highlights several therapeutic strategies for promoting the therapeutic efficacy of MSCs in vitro or in vivo from cell priming to tissue engineering strategies, for use in cardiovascular regeneration.

Download Full-text

Therapeutic Potential of Stem Cells Strategy for Cardiovascular Diseases

Stem Cells International ◽

10.1155/2016/4285938 ◽

2016 ◽

Vol 2016 ◽

pp. 1-10 ◽

Cited By ~ 7

Author(s):

Chang Youn Lee ◽

Ran Kim ◽

Onju Ham ◽

Jihyun Lee ◽

Pilseog Kim ◽

...

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Cardiovascular Diseases ◽

Therapeutic Potential ◽

Therapeutic Strategies ◽

Paracrine Effects ◽

Mortality And Morbidity ◽

The Past ◽

Cell Based Therapy ◽

Immunomodulatory Capacity

Despite development of medicine, cardiovascular diseases (CVDs) are still the leading cause of mortality and morbidity worldwide. Over the past 10 years, various stem cells have been utilized in therapeutic strategies for the treatment of CVDs. CVDs are characterized by a broad range of pathological reactions including inflammation, necrosis, hyperplasia, and hypertrophy. However, the causes of CVDs are still unclear. While there is a limit to the currently available target-dependent treatments, the therapeutic potential of stem cells is very attractive for the treatment of CVDs because of their paracrine effects, anti-inflammatory activity, and immunomodulatory capacity. Various studies have recently reported increased therapeutic potential of transplantation of microRNA- (miRNA-) overexpressing stem cells or small-molecule-treated cells. In addition to treatment with drugs or overexpressed miRNA in stem cells, stem cell-derived extracellular vesicles also have therapeutic potential because they can deliver the stem cell-specific RNA and protein into the host cell, thereby improving cell viability. Here, we reported the state of stem cell-based therapy for the treatment of CVDs and the potential for cell-free based therapy.

Download Full-text

Therapeutic Strategies for Oxidative Stress-Related Cardiovascular Diseases: Removal of Excess Reactive Oxygen Species in Adult Stem Cells

Oxidative Medicine and Cellular Longevity ◽

10.1155/2016/2483163 ◽

2016 ◽

Vol 2016 ◽

pp. 1-11 ◽

Cited By ~ 18

Author(s):

Hyunyun Kim ◽

Jisoo Yun ◽

Sang-Mo Kwon

Keyword(s):

Oxidative Stress ◽

Reactive Oxygen Species ◽

Stem Cells ◽

Stem Cell ◽

Cardiovascular Diseases ◽

Progenitor Cells ◽

Adult Stem Cells ◽

Reactive Oxygen ◽

Therapeutic Strategies ◽

Oxygen Species

Accumulating evidence indicates that acute and chronic uncontrolled overproduction of oxidative stress-related factors including reactive oxygen species (ROS) causes cardiovascular diseases (CVDs), atherosclerosis, and diabetes. Moreover ROS mediate various signaling pathways underlying vascular inflammation in ischemic tissues. With respect to stem cell-based therapy, several studies clearly indicate that modulating antioxidant production at cellular levels enhances stem/progenitor cell functionalities, including proliferation, long-term survival in ischemic tissues, and complete differentiation of transplanted cells into mature vascular cells. Recently emerging therapeutic strategies involving adult stem cells, including endothelial progenitor cells (EPCs), for treating ischemic CVDs have highlighted the need to control intracellular ROS production, because it critically affects the replicative senescence ofex vivoexpanded therapeutic cells. Better understanding of the complexity of cellular ROS in stem cell biology might improve cell survival in ischemic tissues and enhance the regenerative potentials of transplanted stem/progenitor cells. In this review, we will discuss the nature and sources of ROS, drug-based therapeutic strategies for scavenging ROS, and EPC based therapeutic strategies for treating oxidative stress-related CVDs. Furthermore, we will discuss whether primed EPCs pretreated with natural ROS-scavenging compounds are crucial and promising therapeutic strategies for vascular repair.

Download Full-text

Recent Advances in Cardiac Tissue Engineering for the Management of Myocardium Infarction

Cells ◽

10.3390/cells10102538 ◽

2021 ◽

Vol 10 (10) ◽

pp. 2538

Author(s):

Vineeta Sharma ◽

Sanat Kumar Dash ◽

Kavitha Govarthanan ◽

Rekha Gahtori ◽

Nidhi Negi ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Stem Cell ◽

Cardiovascular Diseases ◽

Cardiac Tissue ◽

Microfluidic Devices ◽

Pharmacological Interventions ◽

Physiological Conditions ◽

Myocardium Infarction ◽

Set Up

Myocardium Infarction (MI) is one of the foremost cardiovascular diseases (CVDs) causing death worldwide, and its case numbers are expected to continuously increase in the coming years. Pharmacological interventions have not been at the forefront in ameliorating MI-related morbidity and mortality. Stem cell-based tissue engineering approaches have been extensively explored for their regenerative potential in the infarcted myocardium. Recent studies on microfluidic devices employing stem cells under laboratory set-up have revealed meticulous events pertaining to the pathophysiology of MI occurring at the infarcted site. This discovery also underpins the appropriate conditions in the niche for differentiating stem cells into mature cardiomyocyte-like cells and leads to engineering of the scaffold via mimicking of native cardiac physiological conditions. However, the mode of stem cell-loaded engineered scaffolds delivered to the site of infarction is still a challenging mission, and yet to be translated to the clinical setting. In this review, we have elucidated the various strategies developed using a hydrogel-based system both as encapsulated stem cells and as biocompatible patches loaded with cells and applied at the site of infarction.

Download Full-text

Hard Dental Tissues Regeneration—Approaches and Challenges

Materials ◽

10.3390/ma14102558 ◽

2021 ◽

Vol 14 (10) ◽

pp. 2558

Author(s):

Mihaela Olaru ◽

Liliana Sachelarie ◽

Gabriela Calin

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Stem Cell ◽

Growth Factors ◽

Present Review ◽

Modern Concept ◽

Dental Tissues ◽

Tissue Engineering Approach ◽

Hard Dental Tissues ◽

Review Reports

With the development of the modern concept of tissue engineering approach and the discovery of the potential of stem cells in dentistry, the regeneration of hard dental tissues has become a reality and a priority of modern dentistry. The present review reports the recent advances on stem-cell based regeneration strategies for hard dental tissues and analyze the feasibility of stem cells and of growth factors in scaffolds-based or scaffold-free approaches in inducing the regeneration of either the whole tooth or only of its component structures.

Download Full-text

Magnetic field assisted stem cell differentiation – role of substrate magnetization in osteogenesis

Journal of Materials Chemistry B ◽

10.1039/c5tb00118h ◽

2015 ◽

Vol 3 (16) ◽

pp. 3150-3168 ◽

Cited By ~ 29

Author(s):

Sunil Kumar Boda ◽

Greeshma Thrivikraman ◽

Bikramjit Basu

Keyword(s):

Magnetic Field ◽

Tissue Engineering ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Stem Cell ◽

Cell Differentiation ◽

Bone Tissue Engineering ◽

Human Mesenchymal Stem Cells ◽

Stem Cell Differentiation

Substrate magnetization as a tool for modulating the osteogenesis of human mesenchymal stem cells for bone tissue engineering applications.

Download Full-text

Regenerative medicine and traumatic brain injury: from stem cell to cell-free therapeutic strategies

Regenerative Medicine ◽

10.2217/rme-2021-0069 ◽

2021 ◽

Author(s):

Lianxu Cui ◽

Yasmeen Saeed ◽

Haomin Li ◽

Jingli Yang

Keyword(s):

Traumatic Brain Injury ◽

Stem Cells ◽

Stem Cell ◽

Brain Injury ◽

Regenerative Medicine ◽

Cell Therapy ◽

Therapeutic Strategies ◽

Study Data ◽

Health Concern ◽

Standardized Treatment

Traumatic brain injury (TBI) is a serious health concern, yet there is a lack of standardized treatment to combat its long-lasting effects. The objective of the present study was to provide an overview of the limitation of conventional stem cell therapy in the treatment of TBI and to discuss the application of novel acellular therapies and their advanced strategies to enhance the efficacy of stem cells derived therapies in the light of published study data. Moreover, we also discussed the factor to optimize the therapeutic efficiency of stem cell-derived acellular therapy by overcoming the challenges for its clinical translation. Hence, we concluded that acellular therapy possesses the potential to bring a breakthrough in the field of regenerative medicine to treat TBI.

Download Full-text

Use of adipose-derived stem cells in lymphatic tissue engineering and regeneration

Archives of Plastic Surgery ◽

10.5999/aps.2021.00339 ◽

2021 ◽

Vol 48 (5) ◽

pp. 559-567

Author(s):

Antonio Jorge Forte ◽

Daniel Boczar ◽

Rachel Sarabia-Estrada ◽

Maria T. Huayllani ◽

Francisco R. Avila ◽

...

Keyword(s):

Breast Cancer ◽

Systematic Review ◽

Tissue Engineering ◽

Stem Cells ◽

Stem Cell ◽

Growth Factor ◽

Cochrane Central Register ◽

Adipose Derived Stem Cells ◽

Lymphatic Tissue ◽

Factor C

The potential to differentiate into different cell lines, added to the easy and cost-effective method of extraction, makes adipose-derived stem cells (ADSCs) an object of interest in lymphedema treatment. Our study’s goal was to conduct a comprehensive systematic review of the use of ADSCs in lymphatic tissue engineering and regeneration. On July 23, 2019, using PubMed/MEDLINE, Cochrane Clinical Answers, Cochrane Central Register of Controlled Trials, and Embase databases, we conducted a systematic review of published literature on the use of ADSCs in lymphatic tissue engineering and regeneration. There were no language or time frame limitations, and the following search strategy was applied: ((Adipose stem cell) OR Adipose-derived stem cell)) AND ((Lymphedema) OR Breast Cancer Lymphedema). Only original research manuscripts were included. Fourteen studies fulfilled the inclusion criteria. Eleven studies were experimental (in vitro or in vivo in animals), and only three were clinical. Publications on the topic demonstrated that ADSCs promote lymphangiogenesis, and its effect could be enhanced by modulation of vascular endothelial growth factor-C, interleukin-7, prospero homeobox protein 1, and transforming growth factor-β1. Pilot clinical studies included 11 patients with breast cancer-related lymphedema, and no significant side effects were present at 12-month follow-up. Literature on the use of ADSCs in lymphatic tissue engineering and regeneration demonstrated promising data. Clinical evidence is still in its infancy, but the scientific community agrees that ADSCs can be useful in regenerative lymphangiogenesis. Data collected in this review indicate that unprecedented advances in lymphedema treatment can be anticipated in the upcoming years.

Download Full-text

Biocompatible succinic acid-based polyesters for potential biomedical applications: fungal biofilm inhibition and mesenchymal stem cell growth

RSC Advances ◽

10.1039/c5ra15858c ◽

2015 ◽

Vol 5 (104) ◽

pp. 85756-85766 ◽

Cited By ~ 12

Author(s):

E. Jäger ◽

R. K. Donato ◽

M. Perchacz ◽

A. Jäger ◽

F. Surman ◽

...

Keyword(s):

Tissue Engineering ◽

Stem Cells ◽

Stem Cell ◽

Cell Growth ◽

Mesenchymal Stem Cell ◽

Medical Devices ◽

Biomedical Applications ◽

Growth Promotion ◽

Intrinsic Properties ◽

Biofilm Inhibition

Poly(alkene succinates) are promising materials for specialized medical devices and tissue engineering, presenting intrinsic properties, such as; fungal biofilm inhibition, biocompatibility and stem cells controlled growth promotion.

Download Full-text