scholarly journals Surface Immobilization Chemistry of a Laminin-Derived Peptide Affects Keratinocyte Activity

Coatings ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 560 ◽  
Author(s):  
Nicholas G. Fischer ◽  
Jiahe He ◽  
Conrado Aparicio
Keyword(s):  
Biological Activity ◽  
Cell Adhesion ◽  
Click Chemistry ◽  
Dental Implants ◽  
Photoelectron Spectroscopy ◽  
Artificial Saliva ◽  
Specific System ◽  
Implant Surface ◽  
Substrate Peptide ◽  
Peptide Coatings

Many chemical routes have been proposed to immobilize peptides on biomedical device surfaces, and in particular, on dental implants to prevent peri-implantitis. While a number of factors affect peptide immobilization quality, an easily controllable factor is the chemistry used to immobilize peptides. These factors affect peptide chemoselectivity, orientation, etc., and ultimately control biological activity. Using many different physical and chemical routes for peptide coatings, previous research has intensely focused on immobilizing antimicrobial elements on dental implants to reduce infection rates. Alternatively, our strategy here is different and focused on promoting formation of a long-lasting biological seal between the soft tissue and the implant surface through transmembrane, cell adhesion structures called hemidesmosomes. For that purpose, we used a laminin-derived call adhesion peptide. However, the effect of different immobilization chemistries on cell adhesion peptide activity is vastly unexplored but likely critical. Here, we compared the physiochemical properties and biological responses of a hemidesmosome promoting peptide immobilized using silanization and copper-free click chemistry as a model system for cell adhesion peptides. Successful immobilization was confirmed with water contact angle and X-ray photoelectron spectroscopy. Peptide coatings were retained through 73 days of incubation in artificial saliva. Interestingly, the non-chemoselective immobilization route, silanization, resulted in significantly higher proliferation and hemidesmosome formation in oral keratinocytes compared to chemoselective click chemistry. Our results highlight that the most effective immobilization chemistry for optimal peptide activity is dependent on the specific system (substrate/peptide/cell/biological activity) under study. Overall, a better understanding of the effects immobilization chemistries have on cell adhesion peptide activity may lead to more efficacious coatings for biomedical devices.

scholarly journals Aluminum in Dental Implants: How to Reduce a Potential Risk to Patient’s Health?

2021 ◽  
Vol 6 (1) ◽  
pp. 12
Author(s):  
Željka Petrović ◽  
Ankica Šarić ◽  
Ines Despotović ◽  
Jozefina Katić ◽  
Marin Petković
Keyword(s):  
Dental Implants ◽  
Potential Risk ◽  
Density Functional ◽  
Electrochemical Impedance ◽  
Artificial Saliva ◽  
Biological Effects ◽  
Density Functional Theory Calculation ◽  
Implant Surface ◽  
Hydrolyzed Collagen ◽  
Collagen Molecules

Some commercial dental implants contain aluminum, which represents a potential risk to health, since aluminum is associated with neurodegenerative diseases such as Alzheimer’s disease. Therefore, control of the chemical composition as well as the surface characteristics of implants is necessary, and one approach is functionalization of the implant’s surface by bio(organic) molecules. Hydrolyzed collagen molecules were self-assembled on the titanium implant containing aluminum. Density Functional Theory calculation results indicated an exergonic reaction (ΔG*INT = −6.45 kcal mol−1) between the implant surface and the chosen hydrolyzed collagen molecules, while electrochemical impedance spectroscopy results pointed to improved anti-corrosion properties of the modified implant surface (protective effectiveness, η = 98.5%) compared to the unmodified implant surface. During immersion in an artificial saliva (7 days), the hydrolyzed collagen-modified implant remained stable, which is crucial for minimizing the possible negative biological effects on patient’s health.

scholarly journals A Novel Technique to Increase the Thickness of TiO₂ of Dental Implants by Nd: DPSS Q-sw Laser Treatment

Materials ◽  
2020 ◽  
Vol 13 (18) ◽  
pp. 4178
Author(s):  
Antonio Scarano ◽  
Francesca Postiglione ◽  
Ahmad G. A. Khater ◽  
Faez Saleh Al-Hamed ◽  
Felice Lorusso
Keyword(s):  
Oxide Layer ◽  
Laser Treatment ◽  
Dental Implants ◽  
Photoelectron Spectroscopy ◽  
Clinical Success ◽  
Tissue Response ◽  
Implant Surface ◽  
Study Results ◽  
Dental Implant Surface ◽  
High Bone

High bone–implant contact is a crucial factor in the achievement of osseointegration and long time clinical success of dental implants. Micro, nano, microtopography, and oxide layer of dental implants influence tissue response. The lasers were used for achieving an implant surface with homogeneous micro texturing and uncontaminated surface. The present study aimed to characterize the implant surfaces treated by Nd: DPSS Q-sw Laser treatment compared to machined implants. A total of 10 machined implants and 10 lasered surface implants were evaluated in this study. The implant surfaces were evaluated by X-ray Photoelectron Spectroscopy (XPS), Auger Electron Spectroscopy (AES), and metallography to characterize and measure the thickness of the oxide layer on the implant titanium surface. The machined surfaces showed a non-homogeneous oxide layer ranging between 20 and 30 nm. The lasered implant surfaces showed a homogeneous oxide layer ranging between 400 nm and 460 nm in the area of the laser holes, while outside the layer, thickness ranged between 200 nm and 400 nm without microcracks or evidence of damage. Another exciting result after this laser treatment is a topographically controlled, repeatable, homogeneous, and clean surface. This technique can obtain the implant surface without leaving residues of foreign substances on it. The study results indicate that the use of Nd: DPSS Q-sw laser produces a predictable and reproducible treatment able to improve the titanium oxide layer on the dental implant surface.

Photofunctionalization of Dental Implants

2016 ◽  
Vol 42 (5) ◽  
pp. 445-450 ◽  
Author(s):  
Dennis Flanagan
Keyword(s):  
Biological Activity ◽  
Dental Implants ◽  
Uv Radiation ◽  
Titanium Surface ◽  
Biological Effects ◽  
Wave Length ◽  
Implant Surface ◽  
Dental Implant Surface ◽  
Titanium Dental Implant ◽  
Bone To Implant Contact

After dental implants are manufactured there can be a loss of biological activity that may be reactivated by exposure to ultraviolet (UV) radiation, that is, photofunctionalization. The titanium surface is energy conditioned by UV radiation. This imparts a slight positive surface energy and hydrophilicity to the titanium dental implant surface. This conditioning renews biological activity lost after a shelf life of as little as 2 weeks. The UV radiation has chemical and biological effects on the osseous-implant interface. Photofunctionization for as little as 15 minutes accelerates healing and increases bone to implant contact. The most effective time exposure and UV wave length are in need of identification to produce a surface most conducive for osseointegration.

scholarly journals Macrophagic Inflammatory Response Next to Dental Implants with Different Macro- and Micro-Structure: An In Vitro Study

2021 ◽  
Vol 11 (12) ◽  
pp. 5324
Author(s):  
Maria Menini ◽  
Francesca Delucchi ◽  
Domenico Baldi ◽  
Francesco Pera ◽  
Francesco Bagnasco ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Dental Implants ◽  
In Vitro Study ◽  
Titanium Implants ◽  
Implant Surface ◽  
Bone Implant ◽  
Optimal Outcomes ◽  
Negative Controls ◽  
Inflammatory Effect

(1) Background: Intrinsic characteristics of the implant surface and the possible presence of endotoxins may affect the bone–implant interface and cause an inflammatory response. This study aims to evaluate the possible inflammatory response induced in vitro in macrophages in contact with five different commercially available dental implants. (2) Methods: one zirconia implant NobelPearl® (Nobel Biocare) and four titanium implants, Syra® (Sweden & Martina), Prama® (Sweden & Martina), 3iT3® (Biomet 3i) and Shard® (Mech & Human), were evaluated. After 4 h of contact of murine macrophage cells J774a.1 with the implants, the total RNA was extracted, transcribed to cDNA and the gene expression of the macrophages was evaluated by quantitative PCR (qPCR) in relation to the following genes: GAPDH, YWHAZ, IL1β, IL6, TNFα, NOS2, MMP-9, MMP-8 and TIMP3. The results were statistically analyzed and compared with negative controls. (3) Results: No implant triggered a significant inflammatory response in macrophages, although 3iT3 exhibited a slight pro-inflammatory effect compared to other samples. (4) Conclusions: All the samples showed optimal outcomes without any inflammatory stimulus on the examined macrophagic cells.

scholarly journals The osseointegration and stability of dental implants with different surface treatments in animal models: a network meta-analysis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chun-Ping Hao ◽  
Nan-Jue Cao ◽  
Yu-He Zhu ◽  
Wei Wang
Keyword(s):  
Dental Implants ◽  
Early Stage ◽  
Meta Analysis ◽  
Critical Role ◽  
Cochrane Library ◽  
Missing Teeth ◽  
Treatment Technology ◽  
Implant Surface ◽  
Randomized Controlled ◽  
Early Healing

AbstractDental implants are commonly used to repair missing teeth. The implant surface plays a critical role in promoting osseointegration and implant success. However, little information is available about which implant surface treatment technology best promotes osseointegration and implant stability. The aim of this network meta-analysis was to evaluate the osseointegration and stability of four commonly used dental implants (SLA, SLActive, TiUnite, and Osseotite). The protocol of the current meta-analysis is registered in PROSPERO (International Prospective Register of Systematic Reviews) under the code CRD42020190907 (https://www.crd.york.ac.uk). We conducted a systematic review following PRISMA and Cochrane Recommendations. Medline (PubMed), Cochrane Library, Embase, and the Web of Science databases were searched. Only randomized controlled trials were considered. Twelve studies were included in the current network meta-analysis, eleven studies were included concerning the osseointegration effect and five studies were included for stability analysis (four studies were used to assess both stability and osseointegration). Rank possibility shows that the SLActive surface best promoted bone formation at an early healing stage and TiUnite seemed to be the best surface for overall osseointegration. For stability, TiUnite seemed to be the best surface. The present network meta-analysis showed that the SLActive surface has the potential to promote osseointegration at an early stage. The TiUnite surface had the best effect on osseointegration regarding the overall healing period. The TiUnite surface also had the best effect in stability.

scholarly journals Using “Click” Chemistry to Prepare SAM Substrates to Study Stem Cell Adhesion

Langmuir ◽  
2009 ◽  
Vol 25 (10) ◽  
pp. 5737-5746 ◽  
Author(s):  
Gregory A. Hudalla ◽  
William L. Murphy
Keyword(s):  
Stem Cell ◽  
Cell Adhesion ◽  
Click Chemistry

scholarly journals Influence of Titanium Oxide Pillar Array Nanometric Structures and Ultraviolet Irradiation on the Properties of the Surface of Dental Implants—A Pilot Study

Nanomaterials ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 1458 ◽  
Author(s):  
Leon-Ramos ◽  
Diosdado-Cano ◽  
López-Santos ◽  
Barranco ◽  
Torres-Lagares ◽  
...  
Keyword(s):  
Contact Angle ◽  
Cell Growth ◽  
Dental Implants ◽  
Titanium Oxide ◽  
Ultraviolet Irradiation ◽  
Photoelectron Spectroscopy ◽  
Titanium Implants ◽  
Microwave Source ◽  
Pillar Array ◽  
X Ray

Aim: Titanium implants are commonly used as replacement therapy for lost teeth and much current research is focusing on the improvement of the chemical and physical properties of their surfaces in order to improve the osseointegration process. TiO2, when it is deposited in the form of pillar array nanometric structures, has photocatalytic properties and wet surface control, which, together with UV irradiation, provide it with superhydrophilic surfaces, which may be of interest for improving cell adhesion on the peri-implant surface. In this article, we address the influence of this type of surface treatment on type IV and type V titanium discs on their surface energy and cell growth on them. Materials and methods: Samples from titanium rods used for making dental implants were used. There were two types of samples: grade IV and grade V. In turn, within each grade, two types of samples were differentiated: untreated and treated with sand blasting and subjected to double acid etching. Synthesis of the film consisting of titanium oxide pillar array structures was carried out using plasma-enhanced chemical vapor deposition equipment. The plasma was generated in a quartz vessel by an external SLAN-1 microwave source with a frequency of 2.45 GHz. Five specimens from each group were used (40 discs in total). On the surfaces to be studied, the following determinations were carried out: (a) X-ray photoelectron spectroscopy, (b) scanning electron microscopy, (c) energy dispersive X-ray spectroscopy, (d) profilometry, (e) contact angle measurement or surface wettability, (f) progression of contact angle on applying ultraviolet irradiation, and (g) a biocompatibility test and cytotoxicity with cell cultures. Results: The application of ultraviolet light decreased the hydrophobicity of all the surfaces studied, although it did so to a greater extent on the surfaces with the studied modification applied, this being more evident in samples manufactured in grade V titanium. In samples made in grade IV titanium, this difference was less evident, and even in the sample manufactured with grade IV and SLA treatment, the application of the nanometric modification of the surface made the surface optically less active. Regarding cell growth, all the surfaces studied, grouped in relation to the presence or not of the nanometric treatment, showed similar growth. Conclusions. Treatment of titanium oxide surfaces with ultraviolet irradiation made them change temporarily into superhydrophilic ones, which confirms that their biocompatibility could be improved in this way, or at least be maintained.

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