scholarly journals Potentials of Endophytic Fungi in the Biosynthesis of Versatile Secondary Metabolites and Enzymes

Forests ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1784
Author(s):  
Houda Ben Slama ◽  
Ali Chenari Bouket ◽  
Faizah N. Alenezi ◽  
Zeinab Pourhassan ◽  
Patrycja Golińska ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Culture Media ◽  
Cost Effective ◽  
World Population ◽  
Substantial Cost ◽  
World Population Growth ◽  
Agriculture Industry ◽  
Epigenetic Remodeling ◽  
History Of

World population growth and modernization have engendered multiple environmental problems: the propagation of humans and crop diseases and the development of multi-drug-resistant fungi, bacteria and viruses. Thus, a considerable shift towards eco-friendly products has been seen in medicine, pharmacy, agriculture and several other vital sectors. Nowadays, studies on endophytic fungi and their biotechnological potentials are in high demand due to their substantial, cost-effective and eco-friendly contributions in the discovery of an array of secondary metabolites. For this review, we provide a brief overview of plant–endophytic fungi interactions and we also state the history of the discovery of the untapped potentialities of fungal secondary metabolites. Then, we highlight the huge importance of the discovered metabolites and their versatile applications in several vital fields including medicine, pharmacy, agriculture, industry and bioremediation. We then focus on the challenges and on the possible methods and techniques that can be used to help in the discovery of novel secondary metabolites. The latter range from endophytic selection and culture media optimization to more in-depth strategies such as omics, ribosome engineering and epigenetic remodeling.

scholarly journals OSMAC Strategy Integrated with Molecular Networking for Accessing Griseofulvin Derivatives from Endophytic Fungi of Moquiniastrum polymorphum (Asteraceae)

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7316
Author(s):  
Victor F. Farinella ◽  
Eunizinis S. Kawafune ◽  
Marcelo M. P. Tangerina ◽  
Helori V. Domingos ◽  
Leticia V. Costa-Lotufo ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Cytotoxic Activity ◽  
Endophytic Fungi ◽  
Culture Media ◽  
Malt Extract ◽  
Specific Production ◽  
Spectrometric Data ◽  
Feature Based ◽  
The One ◽  
Peptone Broth

Three endophytic fungi isolated from Moquiniastrum polymorphum (Less.) G. Sancho (Asteraceae) were cultivated using the one strain many compounds (OSMAC) strategy to evaluate the production of griseofulvin derivatives. Extracts obtained were analyzed by HPLC–MS/MS and the chromatographic and spectrometric data used to elaborate a feature-based molecular network (FBMN) through the GNPS platform. This approach allowed the observation of differences such as medium-specific and strain-specific production of griseofulvin derivatives and variations of cytotoxic activity in most extracts. To evaluate the efficiency of the OSMAC approach allied with FBMN analysis in the prospection of compounds of biotechnological interest, griseofulvin and 7-dechlorogriseofulvin were isolated, and the relative concentrations were estimated in all culture media using HPLC–UV, allowing for the inference of the best strain–medium combinations to maximize its production. Malt extract-peptone broth and Wickerham broth media produced the highest concentrations of both secondary metabolites.

Microbial Nanotechnology

2017 ◽  
pp. 102-131
Author(s):  
Vadlapudi Varahalarao ◽  
B.K. Nayak
Keyword(s):  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Biological Activities ◽  
Fungal Endophytes ◽  
Cost Effective ◽  
Rapid Rate ◽  
Negative Effects ◽  
Nanoparticles Synthesis ◽  
Effective Procedures ◽  
Important Branch

Nanoparticles synthesis using microorganisms and others as source has been unexplored and underexploited. The development of green processes for the synthesis of NP is evolving into an important branch of nanotechnology. Fungal endophytes are microorganisms that colonize living, internal tissues of plants without causing any immediate, overtly negative effects, Endophytic fungi are ubiquitous in plant species and are mutualistic to their host, recently, endophytic fungi have been recognized as important sources of a variety of structurally novel active secondary metabolites with anticancer, antimicrobial and other biological activities, Endophytic mediated synthesis of metal nanoparticles is gaining more importance owing to its simplicity, rapid rate of synthesis of NP of attractive and diverse morphologies and elimination of elaborate maintenance of cell cultures and ecofriendliness. Presently, the researchers are looking into the development of cost-effective procedures for producing reproducible, stable and biocompatible metal NPs.

Bioactive Secondary Metabolites from Endophytic Fungi

2020 ◽  
Vol 27 (11) ◽  
pp. 1836-1854 ◽  
Author(s):  
Elena Ancheeva ◽  
Georgios Daletos ◽  
Peter Proksch
Keyword(s):  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Host Plants ◽  
Biological Activities ◽  
Cytotoxic Agents ◽  
Special Focus ◽  
Plant Metabolites ◽  
Direct Production ◽  
Bioactive Secondary Metabolites ◽  
Fungal Genes

Background: Endophytes represent a complex community of microorganisms colonizing asymptomatically internal tissues of higher plants. Several reports have shown that endophytes enhance the fitness of their host plants by direct production of bioactive secondary metabolites, which are involved in protecting the host against herbivores and pathogenic microbes. In addition, it is increasingly apparent that endophytes are able to biosynthesize medicinally important “phytochemicals”, originally believed to be produced only by their host plants. Objective: The present review provides an overview of secondary metabolites from endophytic fungi with pronounced biological activities covering the literature between 2010 and 2017. Special focus is given on studies aiming at exploration of the mode of action of these metabolites towards the discovery of leads from endophytic fungi. Moreover, this review critically evaluates the potential of endophytic fungi as alternative sources of bioactive “plant metabolites”. Results: Over the past few years, several promising lead structures from endophytic fungi have been described in the literature. In this review, 65 metabolites are outlined with pronounced biological activities, primarily as antimicrobial and cytotoxic agents. Some of these metabolites have shown to be highly selective or to possess novel mechanisms of action, which hold great promises as potential drug candidates. Conclusion: Endophytes represent an inexhaustible reservoir of pharmacologically important compounds. Moreover, endophytic fungi could be exploited for the sustainable production of bioactive “plant metabolites” in the future. Towards this aim, further insights into the dynamic endophyte - host plant interactions and origin of endophytic fungal genes would be of utmost importance.

Immunotheraphy in Allergic Diseases

2018 ◽  
Vol 24 (11) ◽  
pp. 1174-1194
Author(s):  
Albert Roger ◽  
Maria Basagana ◽  
Aina Teniente-Serra ◽  
Nathalie Depreux ◽  
Yanina Jurgens ◽  
...  
Keyword(s):  
Specific Immunotherapy ◽  
Allergic Diseases ◽  
Specific Ige ◽  
World Population ◽  
Allergen Specific Immunotherapy ◽  
Routes Of Administration ◽  
Disease Modifying Therapy ◽  
History Of ◽  
Ige Mediated ◽  
Special Case

The prevalence of allergic diseases is increasing worldwide. It is estimated that more than 30% of the world population is now affected by one or more allergic conditions and a high proportion of this increase is in young people. The diagnosis of allergy is dependent on a history of symptoms on exposure to an allergen together with the detection of allergen-specific IgE. Accurate diagnosis of allergies opens up therapeutic options. Allergen specific immunotherapy is the only successful disease-modifying therapy for IgE-mediated allergic diseases. New therapeutic strategies have been developed or are currently under clinical trials. Besides new routes of administration, new types of allergens are being developed. The use of adjuvants may amplify the immune response towards tolerance to the antigens. In this review, we analyze different antigen-specific immunotherapies according to administration route, type of antigens and adjuvants, and we address the special case of food allergy.

Antimicrobial properties of actively purified secondary metabolites isolated from different marine organisms

2020 ◽  
Vol 21 ◽  
Author(s):  
Nilushi Indika Bamunu Arachchige ◽  
Fazlurrahman Khan ◽  
Young-Mog Kim
Keyword(s):  
Secondary Metabolites ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Bacterial Species ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Cost Effective ◽  
Resistance Mechanisms ◽  
Marine Organisms ◽  
Antimicrobial Compounds

Background: The treatment of infection caused by pathogenic bacteria becomes one of the serious concerns globally. The failure in the treatment was found due to the exhibition of multiple resistance mechanisms against the antimicrobial agents. Emergence of resistant bacterial species has also been observed due to prolong treatment using conventional antibiotics. To combat these problems, several alternative strategies have been employed using biological and chemically synthesized compounds as antibacterial agents. Marine organisms considered as one of the potential sources for the isolation of bioactive compounds due to the easily available, cost-effective, and eco-friendly. Methods: The online search methodology was adapted for the collection of information related to the antimicrobial properties of marine-derived compounds. These compound has been isolated and purified by different purification techniques, and their structure also characterized. Furthermore, the antibacterial activities have been reported by using broth microdilution as well as disc diffusion assays. Results: The present review paper describes the antimicrobial effect of diverse secondary metabolites which are isolated and purified from the different marine organisms. The structural elucidation of each secondary metabolite has also been done in the present paper, which will help for the in silico designing of the novel and potent antimicrobial compounds. Conclusion: A thorough literature search has been made and summarizes the list of antimicrobial compounds that are isolated from both prokaryotic and eukaryotic marine organisms. The information obtained from the present paper will be helpful for the application of marine compounds as antimicrobial agents against different antibiotic-resistant human pathogenic bacteria.

scholarly journals Genetic Analysis of a Cohort of 275 Patients with Hyper-IgE Syndromes and/or Chronic Mucocutaneous Candidiasis

2021 ◽  
Author(s):  
Natalie Frede ◽  
Jessica Rojas-Restrepo ◽  
Andrés Caballero Garcia de Oteyza ◽  
Mary Buchta ◽  
Katrin Hübscher ◽  
...  
Keyword(s):  
Screening Method ◽  
Cost Effective ◽  
Illumina Miseq ◽  
Clinical Findings ◽  
Genetic Defects ◽  
Chronic Mucocutaneous Candidiasis ◽  
Mucocutaneous Candidiasis ◽  
Serious Infections ◽  
History Of ◽  
Panel Sequencing

AbstractHyper-IgE syndromes and chronic mucocutaneous candidiasis constitute rare primary immunodeficiency syndromes with an overlapping clinical phenotype. In recent years, a growing number of underlying genetic defects have been identified. To characterize the underlying genetic defects in a large international cohort of 275 patients, of whom 211 had been clinically diagnosed with hyper-IgE syndrome and 64 with chronic mucocutaneous candidiasis, targeted panel sequencing was performed, relying on Agilent HaloPlex and Illumina MiSeq technologies. The targeted panel sequencing approach allowed us to identify 87 (32 novel and 55 previously described) mutations in 78 patients, which generated a diagnostic success rate of 28.4%. Specifically, mutations in DOCK8 (26 patients), STAT3 (21), STAT1 (15), CARD9 (6), AIRE (3), IL17RA (2), SPINK5 (3), ZNF341 (2), CARMIL2/RLTPR (1), IL12RB1 (1), and WAS (1) have been detected. The most common clinical findings in this cohort were elevated IgE (81.5%), eczema (71.7%), and eosinophilia (62.9%). Regarding infections, 54.7% of patients had a history of radiologically proven pneumonia, and 28.3% have had other serious infections. History of fungal infection was noted in 53% of cases and skin abscesses in 52.9%. Skeletal or dental abnormalities were observed in 46.2% of patients with a characteristic face being the most commonly reported feature (23.1%), followed by retained primary teeth in 18.9% of patients. Targeted panel sequencing provides a cost-effective first-line genetic screening method which allows for the identification of mutations also in patients with atypical clinical presentations and should be routinely implemented in referral centers.

scholarly journals Development of Nonlinear Finite Element Models of Mortar-Free Interlocked Single Block Column Subjected to Lateral Loading

2021 ◽  
Author(s):  
Furqan Qamar ◽  
Shunde Qin
Keyword(s):  
Finite Element ◽  
Failure Load ◽  
Cost Effective ◽  
Nonlinear Finite Element ◽  
World Population ◽  
Bond Failure ◽  
Lateral Resistance ◽  
Lateral Strength ◽  
Parametric Studies ◽  
Single Block

AbstractAround the globe, the need for additional housing, due to the increase in world population, has led to the exploration of more cost effective and environmentally friendly forms of construction. Out of many technologies found, mortar-free interlocked masonry systems were developed to eliminate the deficiency of traditional masonry. For such systems against earthquakes, lateral resistance can be enhanced with plaster. But there is a need to further improve the performance of plaster in mortar-free interlocking walls for better ductility. The objective of this study is to develop nonlinear finite element (NLFE) models to explore the likely failure mechanism (e.g. bond failure) of such systems and to do parametric studies more cheaply than constructing many walls. Lateral failure load, load–displacement curves and crack patterns were compared with the experimental results. Parametric studies involving variation in block and plaster compressive strength and plaster thickness were undertaken using TNO DIANA NLFE models. A 150% increase in thickness of plaster only resulted in 28% increase in failure load, and column thickness can be reduced to theoretical 25 mm of blocks with 8 mm of plaster and yet exceed the lateral strength of a 150-mm-thick unplastered column. A cost analysis was also carried out, based on NLFE models, and showed that fibrous plastered column with 25-mm-thickness blocks gave equivalent performance to the 150-mm-thick unplastered column with 67% cost saving.

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