scholarly journals OSMAC Strategy Integrated with Molecular Networking for Accessing Griseofulvin Derivatives from Endophytic Fungi of Moquiniastrum polymorphum (Asteraceae)

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7316
Author(s):  
Victor F. Farinella ◽  
Eunizinis S. Kawafune ◽  
Marcelo M. P. Tangerina ◽  
Helori V. Domingos ◽  
Leticia V. Costa-Lotufo ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Cytotoxic Activity ◽  
Endophytic Fungi ◽  
Culture Media ◽  
Malt Extract ◽  
Specific Production ◽  
Spectrometric Data ◽  
Feature Based ◽  
The One ◽  
Peptone Broth

Three endophytic fungi isolated from Moquiniastrum polymorphum (Less.) G. Sancho (Asteraceae) were cultivated using the one strain many compounds (OSMAC) strategy to evaluate the production of griseofulvin derivatives. Extracts obtained were analyzed by HPLC–MS/MS and the chromatographic and spectrometric data used to elaborate a feature-based molecular network (FBMN) through the GNPS platform. This approach allowed the observation of differences such as medium-specific and strain-specific production of griseofulvin derivatives and variations of cytotoxic activity in most extracts. To evaluate the efficiency of the OSMAC approach allied with FBMN analysis in the prospection of compounds of biotechnological interest, griseofulvin and 7-dechlorogriseofulvin were isolated, and the relative concentrations were estimated in all culture media using HPLC–UV, allowing for the inference of the best strain–medium combinations to maximize its production. Malt extract-peptone broth and Wickerham broth media produced the highest concentrations of both secondary metabolites.

scholarly journals New and bioactive natural products from an endophyte of Panax notoginseng

RSC Advances ◽  
2017 ◽  
Vol 7 (60) ◽  
pp. 38100-38109 ◽  
Author(s):  
Jun Xie ◽  
Ying-Ying Wu ◽  
Tian-Yuan Zhang ◽  
Meng-Yue Zhang ◽  
Wei-Wei Zhu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Natural Products ◽  
Secondary Metabolites ◽  
Antiviral Activity ◽  
Cytotoxic Activity ◽  
Endophytic Fungi ◽  
Panax Notoginseng ◽  
Bioactive Natural Products

Secondary metabolites with cytotoxic activity, antiviral activity and antimicrobial activity from the endophytic fungi of Panax notoginseng.

scholarly journals Potentials of Endophytic Fungi in the Biosynthesis of Versatile Secondary Metabolites and Enzymes

Forests ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 1784
Author(s):  
Houda Ben Slama ◽  
Ali Chenari Bouket ◽  
Faizah N. Alenezi ◽  
Zeinab Pourhassan ◽  
Patrycja Golińska ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Culture Media ◽  
Cost Effective ◽  
World Population ◽  
Substantial Cost ◽  
World Population Growth ◽  
Agriculture Industry ◽  
Epigenetic Remodeling ◽  
History Of

World population growth and modernization have engendered multiple environmental problems: the propagation of humans and crop diseases and the development of multi-drug-resistant fungi, bacteria and viruses. Thus, a considerable shift towards eco-friendly products has been seen in medicine, pharmacy, agriculture and several other vital sectors. Nowadays, studies on endophytic fungi and their biotechnological potentials are in high demand due to their substantial, cost-effective and eco-friendly contributions in the discovery of an array of secondary metabolites. For this review, we provide a brief overview of plant–endophytic fungi interactions and we also state the history of the discovery of the untapped potentialities of fungal secondary metabolites. Then, we highlight the huge importance of the discovered metabolites and their versatile applications in several vital fields including medicine, pharmacy, agriculture, industry and bioremediation. We then focus on the challenges and on the possible methods and techniques that can be used to help in the discovery of novel secondary metabolites. The latter range from endophytic selection and culture media optimization to more in-depth strategies such as omics, ribosome engineering and epigenetic remodeling.

scholarly journals KAPANG ENDOFIT LAUT DARI TUMBUHAN PESISIR TERONG PUNGO (Solanum sp.) DAN POTENSINYA SEBAGAI ANTIBAKTERI

2015 ◽  
Vol 2 (1) ◽  
Author(s):  
Nabila Ukhty
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Staphylococcus Epidermidis ◽  
Inhibition Zone ◽  
Antibacterial Compounds ◽  
Coastal Plant ◽  
The One

Endophytic fungi is the one of the types microbes that lives in the plant tissue. The fungus can produce secondary metabolites potential as a source of antimicrobial and anticancer. The objectives of this study was to the exploration of new antibacterial compounds derived from marine endophytic fungi isolated from coastal plant terong pungo (Solanum sp.). Eight isolates of marine endophytic fungi with different morphology were collected. Endophytic fungus TPL2 was the selected isolate based on antagonism test. The growth curve showed the stationary phase of isolate TPL was on the 9th day to 12th day. Crude extract of endophytic fungi TPL2 showed the antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa with diameter of inhibition zone 4 mm, 6 mm, and 6 mm, respectively for 2 mg extract/well.

scholarly journals SENYAWA METABOLIT SEKUNDER KAPANG ENDOFIT TP6 DAN TPL2 YANG DIISOLASI DARI TUMBUHAN PESISIR TERONG PUNGO (Solanum sp.)

2015 ◽  
Vol 2 (2) ◽  
Author(s):  
Nabila Ukhty ◽  
Kustiariyah Tarman ◽  
Iriani Setyaningsih
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Endophytic Fungi ◽  
Survival Functions ◽  
Coastal Plant ◽  
The One

Secondary metabolites, are produced naturally and serve survival functions for the organisms that producing them. Endophytic fungi is the one of microorganism has a role in producing the secondary metabolites. Endophytic fungi TP6 and TPL2 isolated from coastal plant terong pungo (Solanum sp.). Endophytic fungi TP6 and TPL2 have secondary metabolite, including alcaloid, flavonoid, phenol hidrocuinon, and terpenoid compounds.

scholarly journals Diversity of Marine Macro-Algicolous Endophytic Fungi and Cytotoxic Potential of Biscogniauxia petrensis Metabolites Against Cancer Cell Lines

2021 ◽  
Vol 12 ◽  
Author(s):  
Subhadarsini Sahoo ◽  
Kamalraj Subban ◽  
Jayabaskaran Chelliah
Keyword(s):  
Secondary Metabolites ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Endophytic Fungi ◽  
Tamil Nadu ◽  
Fungal Endophytes ◽  
Cancer Therapeutics ◽  
Cancer Cell Lines ◽  
A431 Cells

Hypersaline environments are known to support diverse fungal species from various orders. The production of secondary metabolites is one of the strategies that fungi adopt to thrive under such extreme environments, bringing up the stress tolerance response. Some such unique secondary metabolites also exhibit clinical significance. The increasing prevalence of drug resistance in cancer therapy demands further exploration of these novel bioactive compounds as cancer therapeutics. In the present study, a total of 31 endophytic fungi harboring inside red, green, and brown marine algae have been isolated and identified. The maximum likelihood analysis and diversity indices of fungal endophytes revealed the phylogenetic relationship and species richness. The genus Aspergillus was found to be the dominating fungus, followed by Cladosporium spp. All the isolated endophytic fungal extracts were tested for their cytotoxicity against HeLa and A431 cancer cell lines. Nine isolates were further analyzed for their cytotoxic activity from the culture filtrate and mycelia extract. Among these isolates, Biscogniauxia petrensis showed potential cytotoxicity with CC50 values of 18.04 and 24.85 μg/ml against HeLa and A431 cells, respectively. Furthermore, the media and solvent extraction optimization revealed the highest cytotoxic active compounds in ethyl acetate extract from the potato dextrose yeast extract broth medium. The compound-induced cell death via apoptosis was 50–60 and 45% when assayed using propidium iodide-live/dead and loss of mitochondrial membrane potential assay, respectively, in HeLa cells. Four bioactive fractions (bioassay-based) were obtained and analyzed using chromatography and spectroscopy. This study reports, for the first time, the cytotoxic activity of an endophytic fungal community that was isolated from marine macro-algae in the Rameswaram coastal region of Tamil Nadu, India. In addition, B. petrensis is a prominent apoptotic agent, which can be used in pharmaceutical applications as a therapeutic.

scholarly journals Natural Products from Endophytic Fungi Associated with Rubiaceae Species

2020 ◽  
Vol 6 (3) ◽  
pp. 128
Author(s):  
Jacqueline Santos Cruz ◽  
Carla Amaral da Silva ◽  
Lidilhone Hamerski
Keyword(s):  
Antioxidant Activity ◽  
Natural Products ◽  
Secondary Metabolites ◽  
Neurodegenerative Diseases ◽  
Cytotoxic Activity ◽  
Endophytic Fungi ◽  
Chemical Diversity ◽  
Pharmacological Properties ◽  
Anti Inflammatory

This review presents the chemical diversity and pharmacological properties of secondary metabolites produced by endophytic fungi associated with various genera of Rubiaceae. Several classes of natural products are described for these endophytes, although, this study highlights the importance of some metabolites, which are involved in antifungal, antibacterial, anti-protozoal activities; neurodegenerative diseases; cytotoxic activity; anti-inflammatory and antioxidant activity; and hyperglycemic control.

Bioactive Secondary Metabolites from Endophytic Fungi

2020 ◽  
Vol 27 (11) ◽  
pp. 1836-1854 ◽  
Author(s):  
Elena Ancheeva ◽  
Georgios Daletos ◽  
Peter Proksch
Keyword(s):  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Host Plants ◽  
Biological Activities ◽  
Cytotoxic Agents ◽  
Special Focus ◽  
Plant Metabolites ◽  
Direct Production ◽  
Bioactive Secondary Metabolites ◽  
Fungal Genes

Background: Endophytes represent a complex community of microorganisms colonizing asymptomatically internal tissues of higher plants. Several reports have shown that endophytes enhance the fitness of their host plants by direct production of bioactive secondary metabolites, which are involved in protecting the host against herbivores and pathogenic microbes. In addition, it is increasingly apparent that endophytes are able to biosynthesize medicinally important “phytochemicals”, originally believed to be produced only by their host plants. Objective: The present review provides an overview of secondary metabolites from endophytic fungi with pronounced biological activities covering the literature between 2010 and 2017. Special focus is given on studies aiming at exploration of the mode of action of these metabolites towards the discovery of leads from endophytic fungi. Moreover, this review critically evaluates the potential of endophytic fungi as alternative sources of bioactive “plant metabolites”. Results: Over the past few years, several promising lead structures from endophytic fungi have been described in the literature. In this review, 65 metabolites are outlined with pronounced biological activities, primarily as antimicrobial and cytotoxic agents. Some of these metabolites have shown to be highly selective or to possess novel mechanisms of action, which hold great promises as potential drug candidates. Conclusion: Endophytes represent an inexhaustible reservoir of pharmacologically important compounds. Moreover, endophytic fungi could be exploited for the sustainable production of bioactive “plant metabolites” in the future. Towards this aim, further insights into the dynamic endophyte - host plant interactions and origin of endophytic fungal genes would be of utmost importance.

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