scholarly journals Genetic and Environmental Variation in Starch Content, Starch Granule Distribution and Starch Polymer Molecular Characteristics of French Bread Wheat

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 205
Author(s):  
Larbi Rhazi ◽  
Benoît Méléard ◽  
Olfa Daaloul ◽  
Guénolé Grignon ◽  
Gérard Branlard ◽  
...  

This study investigates genetic and environmental variation in starch content and characteristics of 14 French bread cultivars. Understanding the impact of these factors on wheat quality is important for processors and especially bakers to maintain and meet the requirements of industrial specifications. Different traits were evaluated: starch content, distribution of starch granules, percentage of amylose and amylopectin and their molecular characteristics (weight-average molar mass, number-average molar mass, polydispersity and gyration radius). Genetic, environment and their interaction had significant effects on all parameters. The relative magnitude of variance attributed to growth conditions, for most traits, was substantially higher (21% to 95%) than that attributed to either genotype (2% to 73%) or G × E interaction (2% to 17%). The largest environmental contribution (95%) to total variance was found for starch dispersity. The highest genetic influence was found for the percentage of A-type starch granules. G × E interaction had relatively little influence (≈7%) on total phenotypic variance. All molecular characteristics were much more influenced by environment than the respective percentages of amylose and amylopectin were. This huge difference in variance between factors obviously revealed the importance of the effect of growing conditions on characteristics of cultivars.

2018 ◽  
Vol 18 (1) ◽  
pp. 10-15
Author(s):  
Wang Yi-Wei ◽  
He Yong-Zhao ◽  
An Feng-Ping ◽  
Huang Qun ◽  
Zeng Feng ◽  
...  

In this study, Chinese yam starch-water suspension (8%) were subjected to high-pressure homogenization (HPH) at 100 MPa for increasing cycle numbers, and its effect of on the physicochemical properties of the starch was investigated. Results of the polarizing microscope observations showed that the starch granules were disrupted (i.e. greater breakdown value) after HPH treatment, followed by a decrease in cross polarization. After three HPH cycles, the crystallinity of starch decreased, while the crystal type remained unaltered. Meanwhile, the contents of rapidly digestible starch and slowly digestible starch were increased. On the contrary, resistant starch content was decreased. Our results indicate that HPH treatment resulted in reduction of starch crystallinity and increase of starch digestibility.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Passakorn Kingwascharapong ◽  
Manat Chaijan ◽  
Supatra Karnjanapratum

AbstractImpact of ultrasound-assisted process (UAP) on yield, functional properties, antioxidant properties and molecular characteristics of protein extracted from Bombay locusts (BL) (Patanga succinta L.) was studied. Different conditions of UAP were implemented for different amplitudes (40–60%) and times (10–30 min) during aqueous extraction. Notably, UAP could enhance yield and protein recovery, compared with those from typical process (TP) (continuously stirred at 100 rpm at room temperature for 1 h). UAP conditions used governed the change of surface hydrophobicity and free α-amino content of BL. UAP could improve solubility of BL, especially at pH levels higher than 2. UAP had no significant (p > 0.05) detrimental effects on foaming capacity and stability of BL. Nevertheless, UAP, particularly at 50–60% amplitudes, affected the emulsion activity and stability of BL. UAP provided BL with high radical scavenging activities and good electron donating ability, especially that from 60% amplitude for 20 min (UAP-60/20). UAP-60/20 showed the impact on change of isoelectric point and molecular characteristic monitored by Fourier transform infrared (FTIR) of BL, compared to those from TP. In addition, BL was also an excellent source of both essential and nonessential amino acids. Therefore, UAP potentially enhanced BL extraction efficiency, resulting the BL with good functional and antioxidative properties.


2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Claudia Maletzki ◽  
Philine Scheinpflug ◽  
Anika Witt ◽  
Ernst Klar ◽  
Michael Linnebacher

This study investigated the impact of immune-related pathway inhibition, among them indolamine 2,3-dioxygenase (IDO), alone and together with immune cells on growth and viability of colorectal cancer (CRC) cells. A panel of patient-derived CRC cell lines with different molecular characteristics (CpG island methylator phenotype, chromosomal, and microsatellite instability) was included. Initial phenotyping of CRC cell lines (n=17) revealed high abundance of immunosuppressive checkpoint-molecules in general, but an individual profile for IDO. Presence of immune-related molecules was independent of the molecular subtype. Selective treatment of CRC cell lines showing high or low IDO expression (n=2 cell lines each) was performed with single agents and combinations of Indoximod, Curcumin, and Gemcitabine with and without the addition of peripheral blood lymphocytes (PBL) in an allogeneic setting. All substances affected CRC cell growth in a cell line specific manner. The combination of Curcumin and Gemcitabine proved to be most effective in tumor cell elimination. Functional read-out analyses identified cellular senescence, after both single and combined treatment. Curcumin alone exerted strong cytotoxic effects by inducing early and late apoptosis. Necrosis was not detectable at all. Addition of lymphocytes generally boosted antitumoral effects of all IDO-inhibitors, with up to 80 % cytotoxicity for the Curcumin treatment. Here, no obvious differences became apparent between individual cell lines. Combined application of Curcumin and low-dose chemotherapy is a promising strategy to kill tumor target cells and to stimulate antitumoral immune responses.


2015 ◽  
Vol 124 ◽  
pp. 254-264 ◽  
Author(s):  
Anne Rieder ◽  
Svein Halvor Knutsen ◽  
Ann-Sissel T. Ulset ◽  
Bjørn E. Christensen ◽  
Roger Andersson ◽  
...  

2021 ◽  
pp. 118742
Author(s):  
Hui You ◽  
Cheng Liang ◽  
Ouling Zhang ◽  
Haoyang Xu ◽  
Liang Xu ◽  
...  

Author(s):  
Н.Т. Чеботарёв ◽  
Н.Н. Шергина

В условиях Республики Коми в полевом стационарном опыте на дерново-подзолистой легкосуглинистой почве изучена эффективность различных доз органических и минеральных удобрений, а также совместного их применения. Исследования проводили в 1978–2019 годах на опытных полях Института агробиотехнологий ФИЦ Коми НЦ УрО РАН. Целью проводимых исследований было изучение влияния комплексного применения удобрений на продуктивность и качество кормовых культур в шестипольном севообороте. Кормовой севооборот имел следующее чередование культур: картофель, викоовсяная смесь с подсевом многолетних трав, многолетние травы 1 г.п., многолетние травы 2 г.п., викоовсяная смесь, картофель. В результате научных исследований (более 40 лет) установлено, что наиболее эффективной была органоминеральная система удобрений, особенно при внесении 80 т/га торфонавозного компоста (ТНК) и минеральных удобрений. Многолетние исследования показали, что наиболее значительные урожаи кормовых культур (в среднем за три ротации) получены при использовании 80 т/га ТНК и NPK: однолетних трав — 4,4 т/га; многолетних трав — 6,2 и картофеля — 7,1 т/га сухого вещества высокого качества. Содержание сухого вещества в клубнях картофеля в вариантах с NPK составило 18,0–18,8%, на органическом фоне — 18,4–18,9 и при комплексном применении удобрений — 17,1–17,7; в контроле — 19,6%. Количество крахмала в картофеле незначительно различалось по вариантам опыта и равнялось 12,6–13,1%. Содержание нитратов не превышало ПДК (250 мг/кг сырой массы). Количество сухого вещества в однолетних и многолетних травах изменялось незначительно и составляло 19,0–19,8 и 25,0–26,8% соответственно. Установлено, что удобрения способствовали повышению содержания сырого протеина в однолетних и многолетних травах до 13,1–15,0% (в контроле — 11,2%) и 8,8–10,6 % (в контроле — 8,1%) соответственно. The impact of various rates of organic and mineral fertilizers was analyzed in the Komi Republic on sod-podzolic soil with low loam content. The research took place at the Institute of Agricultural Biotechnology in 1978–2019. The goal was to test forage crop productivity and quality under fertilization and six-field crop rotation. Crop rotation happened as follows: potatoes, vetch-oat mixture overseeded by perennial grasses, first-year perennial grasses, second-year perennial grasses, vetch-oat mixture, potatoes. For 40 years the combination of mineral and organic fertilizers was the most effective. The highest yields for three rotations were observed under the application of 80 t ha-1 of peat-manure compost and NPK: annual grasses produced 4.4 t ha-1; perennial grasses — 6.2, and potatoes — 7.1 t ha-1 of high-quality dry matter (DM). Potato tubers accumulated 18.0–18.8% of DM under NPK application, 18.4–18.9 — under organic nutrition, 17.1–17.7 — when using complex fertilization, and 19.6% — in the control. Starch content varied within 12.6–13.1% in potatoes. Nitrate content did not exceed the maximum acceptable concentration (250 mg/kg of raw mass). DM concentrations amounted to 19.0–19.8 and 25.0–26.8% in annual and perennial grasses, respectively. Fertilization increased crude protein amount in annual and perennial grasses up to 13.1–15.0% (versus 11.2% in the control) and 8.8–10.6 % (versus 8.1%), respectively.


2003 ◽  
Vol 19 (2) ◽  
pp. 61-76 ◽  
Author(s):  
Tasnim Kallel ◽  
Valérie Massardier-Nageotte ◽  
Mohamed Jaziri ◽  
Jean-François Gérard ◽  
Boubaker Elleuch

PE/PS blends have been extensively studied with the objective of improving their recycling. The objective of the present study was to investigate the effect of potential pollutants on properties of high density polyethylene (HDPE)/polystyrene (PS) plastic blends. The pollutants selected were a polar molecule of low molar mass, i.e. ethylene glycol, and an oil for engine which can be considered as less polar higher molar mass molecules. Such study can be considered for the recycling of polymer wastes from automotive applications. The compatibilizer used for PE/PS blends was a non-grafted Styrene-Ethylene Butene-Styrene copolymer (SEBS). Rheological properties, morphology and mechanical properties were analyzed. Study of the morphologies and of the mechanical properties shows that a small polar molecule such as ethylene glycol can form a third phase whereas an oil can improve compatibilization (lower diameter of the dispersed phase, better adhesion). Morphologies are in good agreement with mechanical behavior. For PE/PS blends, the lower adhesion due to the presence of ethylene glycol induced a decrease of the viscosity and absorbed energy. On the opposite, the presence of oil decreases the diameter of the dispersed phase, which leads to a significant improvement of the impact properties.


Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1743 ◽  
Author(s):  
Qinghua Yang ◽  
Weili Zhang ◽  
Jing Li ◽  
Xiangwei Gong ◽  
Baili Feng

Proso and foxtail millets are widely cultivated due to their excellent resistance to biotic and abiotic stresses and high nutritional value. Starch is the most important component of millet kernels. Starches with different amylose contents have different physicochemical properties. In this study, starches in proso (non-waxy and waxy) and foxtail millets (non-waxy and waxy) were isolated and investigated. All the starch granules had regular polygonal round shapes and exhibited typical “Maltese crosses”. These four starches all showed bimodal size distribution. The waxy proso and foxtail millets had higher weight-average molar mass and branching degree and lower average chain length of amylopectin. These four starches all presented A-type crystallinity; however, the relative crystallinity of waxy proso and foxtail millets was higher. The two waxy millets had higher onset temperature, peak temperature, conclusion temperature, and gelatinization enthalpy. However, the two non-waxy millets had higher setback viscosity, peak time, and pasting temperature. The significantly different physicochemical properties of waxy and non-waxy millet starches resulted in their different functional properties.


2021 ◽  
Author(s):  
Renata Lebecka ◽  
Jadwiga Śliwka ◽  
Anna Grupa-Urbańska ◽  
Katarzyna Szajko ◽  
Waldemar Marczewski

AbstractSoft rot is a bacterial disease that causes heavy losses in potato production worldwide. The goal of this study was to identify quantitative trait loci (QTLs) for potato tuber resistance to bacteriumDickeya solaniand for tuber starch content to study the relationship between these traits. A highly resistant diploid hybrid of potato was crossed with a susceptible hybrid to generate the F1 mapping population. Tubers that were wound-inoculated with bacteria were evaluated for disease severity expressed as the mean weight of rotted tubers, and disease incidence measured as the proportion of rotten tubers. Diversity array technology (DArTseq™) was used for genetic map construction and QTLs analysis. The most prominent QTLs for disease severity and incidence were identified in overlapping regions on potato chromosome IV and explained 22.4% and 22.9% of the phenotypic variance, respectively. The second QTL for disease severity was mapped to chromosome II and explained 16.5% of the variance. QTLs for starch content were detected on chromosomes III, V, VI, VII, VIII, IX, XI, and XII in regions different than the QTLs for soft rot resistance. Two strong and reproducible QTLs for resistance toDickeya solanion potato chromosomes IV and II might be useful for further study of candidate genes and marker development in potato breeding programs. The relationship between tuber resistance to bacteria and the starch content in potato tubers was not confirmed by QTL mapping, which makes the selection of genotypes highly resistant to soft rot with a desirable starch content feasible.


2021 ◽  
Author(s):  
Mohammad Faujul Kabir ◽  
Adam Karami ◽  
Ricardo Cruz-Acuna ◽  
Alena Klochkova ◽  
Reshu Saxena ◽  
...  

ABSTRACTStratified squamous epithelium of the esophagus is comprised of basal keratinocytes that execute a terminal differentiation program in overlying suprabasal and superficial cell layers. Although morphologic progression coupled with expression of specific molecular markers has been characterized along the esophageal epithelial differentiation gradient, the molecular heterogeneity within the cell types along this trajectory has yet to be classified at the level of single cell resolution. To explore the molecular characteristics of esophageal keratinocytes along the squamous differentiation continuum, we performed single cell RNA-Sequencing transcriptomic profiling of 7,972 cells from murine esophageal epithelial sheets. We identified 8 distinct cell clusters in esophageal epithelium, unveiling an unexpected level of diversity, particularly among basal cells. We further mapped the cellular pathways and lineage trajectories within basal, suprabasal, and superficial clusters as well as within the heterogeneous basal cell populations, providing a comprehensive molecular view of esophageal epithelial cells in the context of squamous differentiation. Finally, we explored the impact of tissue aging upon esophageal epithelial cell clusters and demonstrated that mitochondrial dysfunction is a feature of aging in normal esophageal epithelium. These studies provide an unparalleled molecular perspective on murine esophageal keratinocytes that will serve as a valuable resource for dissecting cell type-specific roles in esophageal biology under conditions of homeostasis, aging, and tissue pathology.


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