scholarly journals Chemical and Bioactive Features of Amaranthus caudatus L. Flowers and Optimized Ultrasound-Assisted Extraction of Betalains

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 779
Author(s):  
Custódio Lobo Roriz ◽  
Virginie Xavier ◽  
Sandrina A. Heleno ◽  
José Pinela ◽  
Maria Inês Dias ◽  
...  
Keyword(s):  
Organic Acids ◽  
Pathogenic Bacteria ◽  
Economic Value ◽  
Dry Weight ◽  
Bioactive Molecules ◽  
Ultrasound Assisted Extraction ◽  
Amaranthus Caudatus ◽  
Assisted Extraction ◽  
Ic50 Values ◽  
Ultrasound Assisted

The vibrant colours of many plants are due to secondary metabolites, such as nitrogen-containing compounds, where betacyanins are included. These compounds can be found in plants such as Amaranthus caudatus L. that, due to their high nutritional benefits, have been overproduced, which leads to the accumulation of large amounts of bio-residues. Among these bio-residues, the flowers which have a very intense pink colour and present no economic value or subsequent destination can be exploited as sources of natural colouring agents (betacyanins). This work aimed at characterising the flower’s extract in terms of bioactive molecules such as tocopherols, organic acids, but essentially in terms of betacyanins, in order to obtain a natural colouring agent. For the extraction, ultrasound-assisted extraction (UAE) ideal conditions were obtained using the Response Surface Methodology (RSM), allowing the attainment of an enriched extract of betacyanins in high yields and purity. The obtained extracts were analysed for their bioactive potential, namely antioxidant, antimicrobial and cytotoxic properties. From the obtained results, three isoforms of tocopherols were detected, β-tocopherol (0.884 ± 0.003 mg/100 g dry weight (dw)) being the most abundant one. Regarding the organic acids, oxalic (2.48 ± 0.05 mg/100 g dw), shikimic (0.170 ± 0.003 mg/100 g dw) and traces of fumaric acid were found. Four betacyanins were identified and quantified, namely: amaranthine (171 ± 1 mg/g extract), isoamaranthine (38 ± 1 mg/g extract), betanin (1.6 ± 0.1 mg/g), and isobetanin (1.3 ± 0.1 mg/g extract). The obtained extract also presented antioxidant activity with inhibition concentration (IC50 values) of 29.0 ± 0.4 μg/mL and 114 ± 4 μg/mL for Δt of 60 min and 120 min, respectively in the oxidative haemolysis inhibition assay (OxHLIA) assay. The obtained extract also presented an interesting antibacterial activity with minimum inhibitory concentrations ranging from 5 to 20 mg/mL against pathogenic bacteria and revealed no toxicity for normal cells.

scholarly journals Antioxidant Capacity and Antigenotoxic Effect of Hibiscus sabdariffa L. Extracts Obtained with Ultrasound-Assisted Extraction Process

2020 ◽  
Vol 10 (2) ◽  
pp. 560
Author(s):  
Gregorio Iván Peredo Pozos ◽  
Mario Alberto Ruiz-López ◽  
Juan Francisco Zamora Nátera ◽  
Carlos Álvarez Moya ◽  
Lucia Barrientos Ramírez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Human Lymphocytes ◽  
Dry Weight ◽  
Extraction Process ◽  
Hibiscus Sabdariffa ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Antigenotoxic Effect

Hibiscus sabdariffa (Roselle) is in high demand worldwide due to its beneficial health properties owing to the polyphenols content, mainly in the flower calyx. The objective of this study was to find the best conditions (time and liquid: solid ratio) to extract polyphenols from Roselle using Ultrasound-Assisted Extraction (UAE) (40 kHz, 180 W), with ethanol how solvent; as well as determine the yield of phenols, anthocyanin, flavonoids, tannins, antioxidant activity (DPPH) and antigenotoxic effect (comet assay). A traditional solid-liquid extraction was applied as a reference. Extraction times of 40 and 60 min resulted in the highest polyphenols (13.019 mg GAE/g dry weight (dw)), flavonoids (4.981 CE/g dw), anthocyanins (1.855 mg Cya3GE/g dw), and tannins (0.745 CE/g dw) recoveries and an antioxidant activity (DPPH) of 74.58%. Extracts from white calyces contained similar amounts of phenols and flavonoids, but very little condensed tannins (0.049 CE/g dw) and practically no anthocyanins. Extracts from red and white calyces, showed antigenotoxic activity and repaired capacity of damage caused by mutagens in human lymphocytes.

scholarly journals Ultrasound and Microwave Assisted Extraction of Opuntia Fruit Peels Biocompounds: Optimization and Comparison Using RSM-CCD

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3618 ◽  
Author(s):  
Bruno Melgar ◽  
Maria Inês Dias ◽  
Lillian Barros ◽  
Isabel C.F.R. Ferreira ◽  
Antonio D. Rodriguez-Lopez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Bioactive Molecules ◽  
Specific Response ◽  
Microwave Assisted Extraction ◽  
Ultrasound Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ic50 Values ◽  
Effective Models

Ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) of bioactive compounds, peels from Opuntia engelmannii cultivar (cv.) Valencia were optimized by response surface methodology. Randomized extraction runs were performed for each of the technologies employed in order to build effective models with maximum (bioactive molecules content and yield) and minimum (antioxidant activity) responses. A 5-level, 4-factor central composite design was used to obtain target responses as a function of extraction time (t), solid to liquid ratio (S/L), methanol concentration (metOH), and temperature (T). Specific response optimization for each technology was analyzed, discussed, and general optimization from all the responses together was also gather. The optimum values for each factor were: t = 2.5 and 1.4 min, S/L = 5 and 5 g/L, metOH = 34.6 and 0% of methanol and T = 30 and 36.6 °C, achieving maximum responses of 201.6 and 132.9 mg of betalains/g, 13.9 and 8.0 mg of phenolic acids/g, 2.4 and 1.5 mg of flavonoids/g, 71.8% and 79.1% of extractable solid and IC50 values for the antioxidant activity of 2.9 and 3.6, for UAE and MAE, respectively. The present study suggested UAE as the best extraction system, in order to maximize recovery of bioactive compounds with a high antioxidant activity.

scholarly journals Vine-Canes Valorisation: Ultrasound-Assisted Extraction from Lab to Pilot Scale

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1739 ◽  
Author(s):  
Olena Dorosh ◽  
Manuela M. Moreira ◽  
Francisca Rodrigues ◽  
Andreia F. Peixoto ◽  
Cristina Freire ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Industrial Process ◽  
Dry Weight ◽  
Pilot Scale ◽  
Ultrasound Assisted Extraction ◽  
Wine Production ◽  
Best Value ◽  
Assisted Extraction ◽  
High Antioxidant Activity ◽  
Ultrasound Assisted

Wine production generates large amounts of vine-canes, a devalued by-product that could be used for the recovery of bioactive compounds. In this work, two vine-canes varieties, namely Touriga Nacional (TN) and Tinta Roriz (TR), were submitted to different ultrasound-assisted extraction (UAE) conditions. The highest phenolic and flavonoid content was observed for TR extract obtained at lab-scale without an ice bath and pilot-scale after 60 min of extraction (32.6 ± 2.1 and 26.0 ± 1.5 mg gallic acid equivalent/g dry weight (dw) and 9.5 ± 0.6 and 8.3 ± 0.8 mg epicatechin equivalents/g dw, respectively). Further, all extracts demonstrated a high antioxidant activity to scavenge DPPH free radicals with the best value reached by TR at the lab-scale without an ice bath after 30 min and pilot-scale extraction after 60 min (34.2 ± 2.4 and 33.4 ± 2.1 mg trolox equivalents/g dw, respectively). Extracts phenolic composition were also evaluated by HPLC, demonstrating that resveratrol, myricetin and catechin were the main compounds. According to our knowledge, this is the first time that a pilot scale of UAE of phenolic compounds from vine-canes was performed. This paper represents an important step to the use of UAE as an industrial process to recover bioactive compounds.

scholarly journals Optimization of Ultrasound-Assisted Extraction of Polyphenols from Myrtus communis L. Pericarp

Antioxidants ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 205 ◽  
Author(s):  
Nadia Bouaoudia-Madi ◽  
Lila Boulekbache-Makhlouf ◽  
Khodir Madani ◽  
Artur M.S. Silva ◽  
Sofiane Dairi ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Irradiation Time ◽  
Dry Weight ◽  
Total Phenolic ◽  
Ionization Mass ◽  
Myrtus Communis ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Gallic Acid Equivalent

Response surface methodology (RSM) was used to optimize the extraction of phenolics from pericap of Myrtus communis using ultrasound-assisted extraction (UAE). The results were compared with those obtained by microwave-assisted extraction (MAE) and conventional solvent extraction (CSE) methods. The individual compounds of the optimized extract obtained by UAE were identified by ultra-high-performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (UHPLC-DAD-ESI-MSn). The yield of total phenolic compounds (TPC) was affected more significantly by ethanol concentration, irradiation time, liquid solvent-to-solid ratio (p < 0.0001) and amplitude (p = 0.0421) and optimal parameters conditions set by the RSM model were 70% (v/v), 7.5 min and 30%, respectively. The experimental yield of TPC (241.66 ± 12.77 mg gallic acid equivalent/g dry weight) confirmed the predicted value (235.52 ± 9.9 mg gallic acid equivalent/g dry weight), allowing also to confirm the model validity. Under optimized conditions, UAE was more efficient than MAE and CSE in extracting antioxidants, which comprised mostly myricetin glycosides. Globally, the present work demonstrated that, compared to MAE and CSE, UAE is an efficient method for phenolic extraction from M. communis pericarp, enabling to reduce the working time and the solvent consumption.

scholarly journals A Green Ultrasound-Assisted Extraction Optimization of the Natural Antioxidant and Anti-Aging Flavonolignans from Milk Thistle Silybum marianum (L.) Gaertn. Fruits for Cosmetic Applications

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 304 ◽  
Author(s):  
Samantha Drouet ◽  
Emilie A. Leclerc ◽  
Laurine Garros ◽  
Duangjai Tungmunnithum ◽  
Atul Kabra ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Dry Weight ◽  
International Standards ◽  
In Vitro Assays ◽  
Milk Thistle ◽  
Silybum Marianum ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Silybum marianum (L.) Gaertn. (aka milk thistle) constitutes the source of silymarin (SILM), a mixture of different flavonolignans and represents a unique model for their extraction. Here we report on the development and validation of an ultrasound-assisted extraction (UAE) method of S. marianum flavonolignans follow by their quantification using LC system. The optimal conditions of this UAE method were: aqueous EtOH 54.5% (v/v) as extraction solvent, with application of an ultrasound (US) frequency of 36.6 kHz during 60 min at 45 °C with a liquid to solid ratio of 25:1 mL/g dry weight (DW). Following its optimization using a full factorial design, the extraction method was validated according to international standards of the association of analytical communities (AOAC) to ensure precision and accuracy in the quantitation of each component of the SILM mixture. The efficiency of this UAE was compared with maceration protocol. Here, the optimized and validated conditions of the UAE allowed the highest extraction yields of SILM and its constituents in comparison to maceration. During UAE, the antioxidant capacity of the extracts was retained, as confirmed by the in vitro assays CUPRAC (cupric ion reducing antioxidant capacity) and inhibition of AGEs (advanced glycation end products). The skin anti-aging potential of the extract obtained by UAE was also confirmed by the strong in vitro cell-free inhibition capacity of both collagenase and elastase. To summarize, the UAE procedure presented here is a green and efficient method for the extraction and quantification of SILM and its constituents from the fruits of S. marianum, making it possible to generate extracts with attractive antioxidant and anti-aging activities for future cosmetic applications.

scholarly journals Optimization of Ultrasound Assisted Extraction of Phenolic Compounds from Orange By-Product

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 49
Author(s):  
María del Carmen Razola-Díaz ◽  
Eduardo Jesús Guerra-Hernández ◽  
Celia Rodríguez-Pérez ◽  
Ana María Gómez-Caravaca ◽  
Belén García-Villanova ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Orange Peel ◽  
Dry Weight ◽  
Total Phenolic ◽  
Total Phenolic Compounds ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Quantitative Analyses ◽  
Ultrasound Assisted

Orange peel (OP) is the main by-product from orange juice industry. OP is a known source of bioactive compounds and is widely studied for its antioxidant, anti-inflammatory, anti-cancer, anti-rheumatic, anti-diabetic and cardioprotective activities. Thus, this research focuses on the establishments of ultrasound-assisted extraction of phenolic compounds in orange peel using a sonotrode; objective framed in the European SHEALTHY (non-thermal physical technologies to preserve healthiness of fresh and minimally processed fruit and vegetables) project. For this purpose, a Box Behnken design of 27 experiments was carried out with 4 independent factors (ratio ethanol/water, time (min), amplitude (%) and pulse (%)). Quantitative analyses of total phenolic compounds (TPC) were performed by Folin-Ciocalteu method and the antioxidant activity was measured by ABTS and DPPH methods. The validity of the experimental design was confirmed by ANOVA and the optimal sonotrode extraction conditions were obtained by response surface methodology. The optimal extracts were characterized by HPLC coupled to mass spectrometer detectors. The highest phenolic content and antioxidant activity was obtained using 45/55 ethanol/water (v/v), 35 min, amplitude 90% (110 W) and pulse 100%. The established method allows the extraction of 30.42 mg of gallic acid equivalents/g dry weight of total phenolic compounds from OP; this value suppose an increment up to 60% higher than conventional extraction.

scholarly journals Ultrasound-Assisted Extraction Optimization of Phenolic Compounds from Citrus latifolia Waste for Chitosan Bioactive Nanoparticles Development

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3541 ◽  
Author(s):  
Nelly Medina-Torres ◽  
Hugo Espinosa-Andrews ◽  
Stéphane Trombotto ◽  
Teresa Ayora-Talavera ◽  
Jesús Patrón-Vázquez ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Chitosan Nanoparticles ◽  
Sinapic Acid ◽  
Value Added ◽  
Dry Weight ◽  
Total Phenolic ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Citrus Latifolia ◽  
Ultrasound Assisted

Bioactive Phenols-loaded chitosan nanoparticles (PL-CNps) were developed by ionic gelation from Persian lemon (Citrus latifolia) waste (PLW) and chitosan nanoparticles. Response Surface Methodology (RSM) was used to determine the optimal Ultrasound-Assisted Extraction (UAE) conditions for the total phenolic compounds (TPC) recovery from PLW (58.13 mg GAE/g dw), evaluating the ethanol concentration, extraction time, amplitude, and solid/liquid ratio. Eight compounds expressed as mg/g dry weight (dw) were identified by ultra-performance liquid chromatography coupled photo diode array (UPLC-PDA) analysis: eriocitrin (20.71 ± 0.09), diosmin (18.59 ± 0.13), hesperidin (7.30 ± 0.04), sinapic acid (3.67 ± 0.04), catechin (2.92 ± 0.05), coumaric acid (2.86 ± 0.01), neohesperidin (1.63 ± 0.00), and naringenin (0.44 ± 0.00). The PL-CNps presented size of 232.7 nm, polydispersity index of 0.182, Z potential of −3.8 mV, and encapsulation efficiency of 81.16%. The results indicated that a synergic effect between phenolic compounds from PLW and chitosan nanoparticles was observed in antioxidant and antibacterial activity, according to Limpel’s equation. Such results indicate that PLW in such bioprocesses shows excellent potential as substrates for the production of value-added compounds with a special application for the food industry.

scholarly journals Optimization of Ultrasound Assisted Extraction (UAE) of Kinsenoside Compound from Anoectochilus roxburghii (Wall.) Lindl by Response Surface Methodology (RSM)

Molecules ◽  
2020 ◽  
Vol 25 (1) ◽  
pp. 193 ◽  
Author(s):  
Biyun Yang ◽  
Mengyuan Zhang ◽  
Haiyong Weng ◽  
Yong Xu ◽  
Lihui Zeng
Keyword(s):  
Response Surface ◽  
High Performance ◽  
Dry Weight ◽  
Methanol Concentration ◽  
Ultrasonic Power ◽  
Ultrasound Assisted Extraction ◽  
Solid Ratio ◽  
Assisted Extraction ◽  
Anoectochilus Roxburghii ◽  
Ultrasound Assisted

The purpose of this study was to establish an extraction method for the kinsenoside compound from the whole plant Anoectochilus roxburghii. Ultrasound assisted extraction (UAE) and Ultra-high performance liquid chromatography (UPLC) method were used to extract and determine the content of kinsenoside, while response surface method (RSM) was used to optimize the extraction process. The best possible range for methanol concentration (0–100%), the liquid-solid ratio (5:1–30:1 mL/g), ultrasonic power (240–540 W), duration of ultrasound (10–50 min), ultrasonic temperature (10–60 °C), and the number of extractions (1–4) were obtained according to the single factor experiments. Then, using the Box-Behnken design (BBD) of response surface analysis, the optimum extraction conditions were obtained with 16.33% methanol concentration, the liquid-solid ratio of 10.83:1 mL/g and 35.00 °C ultrasonic temperature. Under these conditions, kinsenoside extraction yield reached 32.24% dry weight. The best conditions were applied to determine the kinsenoside content in seven different cultivation ages in Anoectochilus roxburghii.

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